529904 6922twf. doc/002 A7 B7 五、發明說明（I ) 本發明是有關於一種捕捉昆蟲之方法，且特別是有關 於一種微生物媒介以吸引蚊蟲，以便於達到控制目的或殺 滅目的之方法及裝置。 從古至今’人類一直想要有效地防治昆蟲，特別是對 人類有害的昆蟲，例如蚊蟲。蚊蟲之所以對人類有害主要 是因爲蚊蟲會幫助散布傳染疾病，諸如瘧疾和黃熱病等會 經由蚊蟲而散佈傳染；因此，爲了要防止蚊蟲相關之疾病 之傳播，世界各國的公共衛生當局均一直持續不斷地致力 於蚊蟲防治，以消除蚊蟲相關之疾病。除了疾病防治之原 因外，蚊蟲叮咬而引致之痛癢及不便，也是另一個殺滅蚊 蟲之原因。由於完全滅絕蚊蟲是幾乎不可能達成之目標， 因此，長久以來人類一直不斷在尋找有效防制蚊蟲之方法 及機制，包括如何吸引蚊蟲以便於捕捉及殺滅蚊蟲。 爲了從宿主身上吸取血液來提供卵發育所需之養分， 雌蚊會四處飛行以尋找宿主（host)吸食血液；一般而言， 蚊蟲乃是利用宿主所散發出的特殊化學成份來偵測鎖定宿 主，而人類宿主身上散發的特殊化學成份，也就是吸引雌 蚊之引誘劑（Attractant)，乃爲一些易揮發物。這些易揮發 物包含多達300種至400種化合物，其來源可能是宿主之 皮腺分泌物，皮膚上微生物之分解產物，或兩者皆是。雌 蚊是利用其嗅覺結構來偵測鎖定宿主，其可在90英尺遠 處探測到宿主身上散發出之引誘劑，並進而鎖定宿主。 目前常見防制蚊蟲的方法，多半是經由化學方法來殺 滅整個蚊蟲群體，或是試圖消滅它們的繁殖棲息地。這些 4 本紙張尺度適用中國國家標準（CNS)A4規格（210 X 297公釐） (請先閱讀背面之注意事項再填寫本頁) . 線. 經濟部智慧財產局員工消費合作社印製 529904 6922twf.doc/002 A7 B7 經濟部智慧財產局員工消費合作社印製 五、發明說明（2) 方法不但麻煩又低效率，而且常常是具有生態破壞性的， 特別是會引致有毒化學物至周遭環境而造成生態污染。另 外可行的方法是製造可以捕捉蚊蟲之陷阱；其中各種不同 之機制均曾用於製造蚊蟲陷阱，包括光線、熱度、二氧化 碳、八稀醇（Octenol)、水蒸氣和乳酸，皆曾被考慮作爲引 誘劑。 本發明的主要目的是在提供一種防治雌蚊之方法和裝 置，其機制包括利用一細菌分解物和一酵母菌發酵液作爲 一雌蚊誘餌，該細菌分解物和該酵母菌發酵液的結合可同 時吸引嗜血雌蚊以及非嗜血期之蚊蟲。本發明並運用誘餌 與熱度的結合模擬宿主，達成非常高的吸引率。本發明之 方法包括吸引以捕捉蚊蟲，或是先吸引捕捉並消滅蚊蟲， 以達成防制蚊蟲目的。 本發明的另一目的是在提供一種微生物媒介之誘蚊方 法及裝置，其機制包括使用一蚊蟲誘餌，以模擬人體體表 溫度和人體散發之氣味來吸引雌蚊，並進而達到控制及誘 捕蚊蟲之目的。該裝置中包栝一可調控加熱裝置，會提供 適當的熱度，來幫助微生物的培養和幫助一蚊蟲誘餌蒸 發。該蚊蟲誘餌至少包含一細菌分解物和一酵母菌發酵 液；該蚊蟲誘餌對於人類不具毒性，而且該蚊蟲誘餌並非 人工合成，乃是由其包含的發酵液或細菌分解物等以及其 自然代謝產物所組成。此外，本發明也可以結合通電柵格 或含殺蟲劑濾網之使用以殺滅被引誘之雌蚊。。 依照本發明一較佳實例，本發明之裝置爲一可攜帶移 5 本紙張尺度適用中關家標準（CNS)A4規格（21G X 297公爱了 -----r---.-------------訂---------線 (請先閱讀背面之注意事項再填寫本頁) 529904 -6 922twf. doc/002 A7 B7 五、發明說明（5 ) 動式蚊蟲控制系統，可以在室內或在戶外使用來捕捉蚊 蟲。該裝置包含一上層隔間（Upper Compartment)，該上層 隔間有複數個導入隧孔，可讓蚊蟲飛入而被陷於該上層隔 間中；一底層隔間（Bottom Compartment);和一雙頭螺旋 連結座(Thumbscrew Container)，該雙頭螺旋連結座至少包 括一頂指螺旋，一底指螺旋和一位於其間之柵格，以連接該 上層隔間和該底層隔間。該蚊蟲誘引系統更包括一第一固 定裝置以固定一第一容器，該第一容器至少包含一酵母菌 發酵液；一第二固定裝置以固定一第二容器，該第二容器 至少包含一細菌培養基；以及可調控的加熱裝置，可以提 供熱以幫助細菌培養和酵母菌發酵。 爲讓本發明之上述和其他目的、特徵、和優點能更曰月 顯易懂，下文特舉一較佳實例，並配合所附圖式，作詳細 說明如下： 圖式之簡單說明： 第1圖是依照本發明一較佳實施例的一種裝置之 圖。 經濟部智慧財產局員工消費合作社印製 (請先閱讀背面之注意事項再填寫本頁)529904 6922twf. Doc / 002 A7 B7 V. Description of the invention (I) The present invention relates to a method for catching insects, and in particular to a method for attracting mosquitoes with a microbial vector in order to achieve control or killing purposes, and Device. Since ancient times, human beings have wanted to effectively control insects, especially those harmful to humans, such as mosquitoes. Mosquitoes are harmful to humans mainly because mosquitoes can help spread infectious diseases, such as malaria and yellow fever, which can spread through mosquitoes; therefore, in order to prevent the spread of mosquito-related diseases, public health authorities in all countries around the world have been continuing Continuously working on mosquito control to eliminate mosquito-related diseases. In addition to the causes of disease control, the itch and inconvenience caused by mosquito bites is another reason to kill mosquitoes. Since the complete elimination of mosquitoes is an almost impossible goal, human beings have been searching for effective methods and mechanisms for controlling mosquitoes for a long time, including how to attract mosquitoes in order to catch and kill mosquitoes. In order to draw blood from the host to provide nutrients needed for egg development, female mosquitoes fly around to find the host to suck blood; in general, mosquitoes use special chemical components emitted by the host to detect and lock the host The special chemical components emitted by the human host, that is, the attractant that attracts female mosquitoes, are volatiles. These volatiles contain up to 300 to 400 compounds, which may originate from the dermal secretions of the host, decomposition products of microorganisms on the skin, or both. Female mosquitoes use their olfactory structure to detect locked hosts. They can detect the attractant emitted from the host at a distance of 90 feet, and then lock the host. At present, the common methods for controlling mosquitoes are to kill the entire mosquito population through chemical methods, or to try to destroy their breeding habitats. These 4 paper sizes are in accordance with Chinese National Standard (CNS) A4 (210 X 297 mm) (please read the notes on the back before filling out this page). Line. Printed by the Intellectual Property Bureau Staff Consumer Cooperative of the Ministry of Economic Affairs 529904 6922twf. doc / 002 A7 B7 Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs. 5. Description of the Invention (2) The method is not only cumbersome and inefficient, but also often ecologically destructive, especially causing toxic chemicals to the surrounding environment. Ecological pollution. Another feasible method is to make traps that can catch mosquitoes; various mechanisms have been used to make mosquito traps, including light, heat, carbon dioxide, octenol, water vapor, and lactic acid, all of which have been considered as lures. Agent. The main purpose of the present invention is to provide a method and a device for controlling female mosquitoes. The mechanism includes using a bacterial decomposed product and a yeast fermentation broth as a female mosquito bait. Attracts both female bloodthirsty mosquitoes and non-bloodthirsty mosquitoes. The invention also uses a combination of bait and heat to simulate the host, and achieves a very high attraction rate. The method of the present invention includes attracting to catch mosquitoes, or attracting and killing mosquitoes first to achieve the purpose of preventing mosquitoes. Another object of the present invention is to provide a method and a device for attracting mosquitoes by using a microbial agent. The mechanism includes the use of a mosquito bait to simulate the temperature of the human body surface and the odor emitted by the human body to attract female mosquitoes. Purpose. The device contains a controllable heating device that will provide appropriate heat to help the cultivation of microorganisms and the evaporation of a mosquito bait. The mosquito bait contains at least a bacterial decomposition product and a yeast fermentation liquid; the mosquito bait is not toxic to humans; and the mosquito bait is not artificially synthesized, but is composed of the fermentation liquid or bacterial decomposition product and its natural metabolites. Composed of. In addition, the present invention can also be combined with the use of energized grids or pesticide-containing filters to kill attracted female mosquitoes. . According to a preferred embodiment of the present invention, the device of the present invention is a portable 5 paper standard applicable to the China Family Standard (CNS) A4 specification (21G X 297) ----- r ---.-- ----------- Order --------- line (please read the notes on the back before filling this page) 529904 -6 922twf. Doc / 002 A7 B7 V. Description of the invention ( 5) The movable mosquito control system can be used indoors or outdoors to capture mosquitoes. The device includes an upper compartment (Upper Compartment), the upper compartment has a plurality of introduction tunnels, which can allow mosquitoes to fly in and be trapped In the upper compartment; a bottom compartment (Bottom Compartment); and a double-screw container (Thumbscrew Container), the double-head spiral connection includes at least a top finger spiral, a bottom finger spiral and a grid in between To connect the upper compartment and the bottom compartment. The mosquito attracting system further includes a first fixing device to fix a first container, the first container contains at least a yeast fermentation liquid; a second fixing device to A second container is fixed, the second container contains at least a bacterial culture medium; The heating device can provide heat to help bacterial culture and yeast fermentation. In order to make the above and other objects, features, and advantages of the present invention more comprehensible, a preferred example is given below with the accompanying The drawings are described in detail as follows: Brief description of the drawings: Figure 1 is a diagram of a device according to a preferred embodiment of the present invention. Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs (please read the precautions on the back first) (Fill in this page again)
第2A圖是依照本發明一較佳實施例的該裝置之 圖，第2B圖是如第2A圖所示依照本發明一較佳實施例 的該裝置之部分爆炸圖。 圖式之標記說明： 1〇〇、200 :系統裝置 102、202 :上層隔間（Upper Compartment) 104、204 :底層隔間（Bottom Compartment) 6 本紙張尺度適用中國^^票準（CNS)‘規格⑽χ撕公髮） 529904 6922twf. doc/002 A7 B7 經濟部智慧財產局員工消費合作社印製 五、發明說明（> ) 106、206 :雙頭旋螺連結座(Thumbscrew Container) 108 :頂蓋 110 :導入隧孔 112、212 :柵格 114 :加熱裝置 116、216 :金屬加熱器 120、220 :第一容器 122、222 :第二容器 224 :格狀固定裝置 226 :頂指螺旋226 228 :底指螺旋 230 ··第一^ 〇 形環(0-ring) 232 :第二0形環 234 :收集網 236 :固定附屬裝置 238 :可移動的底拴蓋 218 :反向漏斗 實例 某些本來就存在於人類汗液中之桿狀細菌，已知會生 產甲硫醇（Methanethiols)，而散發出如同乾酪或人類腳底 的氣味。這些微生物可能是導致人類汗水散發氣味之原 因，而目前已有實驗證明人類汗液經某些細菌代謝以後， 產生易揮發性物質，對雌蚊有頗強之吸引力。因此，若能 辨認分離出這些細菌，就可進而使用這些細菌來產生蚊蟲 7 •卜--7.-------^------訂---------線« (請先閱讀背面之注意事項再填寫本頁) 丨 本紙張尺度適用中國國家標準（CNS)A4規格（210 X 297公釐） 529904 6 9 2 21 w f. d 〇 c / 0 0 2 529904 6 9 2 21 w f. d 〇 c / 0 0 2 經濟部智慧財產局員工消費合作社印製 A7 B7 五、發明說明（/Γ) 引誘劑，以模擬人類氣味來引誘嗜血性雌蚊。 一般而言，雄蚊唯一的食物來源是花蜜。而某些種類 的雌蚊除了吸血之外，也會在吸血前先飽餐一頓蜜糖餐； 這是因爲糖類是雌蚊飛行尋找宿主時的精力來源，而且這 會影響雌蚊對宿主的引誘劑之反應靈敏度。另外，有實驗 報告指出二氧化碳（co2)對於蚊蟲有著普遍（非限於特定 種類）的吸引力，且該蚊蟲吸引力與二氧化碳之濃度成正 比。本發明中之蚊蟲誘餌包括至少一人體氣味模擬物，以 模擬人類散發之易揮發物，一蜜糖來源和一二氧化碳供給 源。該人體氣味模擬物包含一特定細菌分解物，而一以果 汁做酵母菌發酵的培養基可提供穩定之二氧化碳來源，並 作爲蜜糖來源。 實驗資料 實驗使用之蚊蟲爲埃及斑蚊aegj/pi/)，培養於 23-25°C，60-80%之相對濕度以及十二小時光/十二小時 暗的環境。成蚊置於大小30立方公分(cm3)罩以布幕之籠 內並餵以1〇%(ν/ν)蔗糖水溶液。在進行誘蚊測試實驗時雌 蚊先做4-8天飢餓處理，然後釋放到一具雙孔的氣味測量 儀(Olfactometer)，該氣味測量儀包含一飛行室（1.6m x0.6m x0.6m)以供釋放蚊子飛行。空氣唧筒吸取新鮮空氣經由活 性炭過濾器，再通過二道的蒸餾水，而經由雙孔中之一孔 (5公分直徑，分開30公分左右）釋放到飛行室中。一氣味 來源是置於另一孔中，而經由一水循環系統溫度保持在30 °C。室溫保持在23-25t。誘蚊測試實驗之觀察時間是在 8 本紙張尺度適用中國國家標準（CNS)A4規格（210 X 297公爱） ---τιν----——----丨—訂---------線· (請先閱讀背面之注意事項再填寫本頁) 529904 6922twf.doc/002 529904 6922twf.doc/002 經濟部智慧財產局員工消費合作社印製 A7 B7 五、發明說明（& ) 釋放蚊蟲後15分鐘觀察。在連續的各實驗之間，該氣味 測試儀之各部份都用75%之酒精來淸潔並用強風吹二十 分鐘以去除氣味。此外，爲了避免方向性相關之未知因素 造成偏差，每個試驗中對照與控制組之位置是輪流交替於 雙孔之間。 實驗用之細菌爲桿狀（Coryneform)細菌之兩株，爲 (BE)和 5. //似似（BL)，分別從 德國Sammlung von Mikrooranismen公司和德國布魯茲維 克Zellkulturen GmbH公司和位於新竹之食品工業發展&硏 究所所購買。細菌可用三種不同的培養液加以培養：TSB、 MB和NB。這三種不同之培養液之成份分列表如下：TSB (15公克的胰化蛋白質（Tryptone)+ 5公克的大豆蛋白質 (Soytone)+ 5公克的NaCl/每公升，而水溶液之酸鹼度値 7.3); MB (50公克的奶粉+ 2.5公克的消化蛋白質(Peptone)/ 每公升，水溶液之酸鹼度値7.2);和NB (5公克的消化蛋 白質+ 1.5公克的牛肉抽取物（Beet extracts ) + 1.5公克的 酵母菌抽取物（Yeast extracts) + 5公克的NaCl/每公升， 水溶液之酸鹼度値7.4);細菌培養於恆溫30°C，每分鐘200 轉數之搖瓶環境下培養二天之久。 爲了檢測不同的細菌株和不同培養液的組合，何者具 有最高效率的蚊蟲吸引力，取4毫升已培育兩天的不同組 合細菌培養液置入氣味測量儀中加以試驗。 如表一所示，大部分這些菌株，不論成長在那一種培 養液中均能吸引蚊蟲；除了菌株BE9585對蚊蟲沒有吸引 9 本紙張尺度適用中國國家標準（CNS)A4規格（210 X 297公釐） •---ί, - — L - I — - · I l· I I I- I I ^ — — — — — — — — I I I I I u— . (請先閱讀背面之注意事項再填寫本頁) 529904 6922twf. doc/002 A7 B7 五、發明說明（q) 力。其中只有菌株BE9586是唯一的菌株，不論配合任意 三種不同之培養液都顯示對雌蚊蟲具吸引力，而且以培養 在NB中會對蚊蟲產生最高的吸引力。大致而言，若缺少 加熱裝置，則對蚊蟲的吸引力不會高於30%。 表一不同的細菌和培養液的組合對成雌蚊之吸 引力Figure 2A is a diagram of the device according to a preferred embodiment of the present invention, and Figure 2B is a partially exploded view of the device according to a preferred embodiment of the present invention as shown in Figure 2A. Explanation of the marks of the drawings: 100, 200: system devices 102, 202: upper compartment (Upper Compartment) 104, 204: bottom compartment (Bottom Compartment) 6 This paper size applies to China ^^ 票 准 (CNS) ' Specification ⑽χTear public hair) 529904 6922twf. Doc / 002 A7 B7 Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs. 5. Description of the invention (>) 106, 206: Double-screw container (Thumbscrew Container) 108: Top cover 110: introduction tunnel 112, 212: grid 114: heating device 116, 216: metal heater 120, 220: first container 122, 222: second container 224: grid-like fixing device 226: top finger screw 226 228: Bottom finger helix 230 · First ^ 〇 ring (0-ring) 232: second 0 ring 234: collection net 236: fixed attachment 238: removable bottom bolt cover 218: reverse funnel example some originally Bacillus bacteria, found in human sweat, are known to produce Methanethiols and emit an odor like cheese or the soles of human feet. These microorganisms may be responsible for the smell of human sweat. At present, experiments have shown that human sweat is metabolized by certain bacteria to produce volatile substances, which has a strong appeal to female mosquitoes. Therefore, if these bacteria can be identified and separated, then these bacteria can be used to generate mosquitoes 7 • Bu--7 .------- ^ ------ Order --------- Line «(Please read the precautions on the back before filling this page) 丨 This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) 529904 6 9 2 21 w f. D 〇c / 0 0 2 529904 6 9 2 21 w f. D oc / 0 0 2 A7 B7 printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs V. Description of the invention (/ Γ) Attractants, which mimic human odors to attract bloodthirsty female mosquitoes. In general, the only food source for male mosquitoes is nectar. In addition to certain types of female mosquitoes, in addition to sucking blood, they will also eat a honey meal before sucking blood; this is because sugar is the source of energy for female mosquitoes to fly to find the host, and this will affect the attractant of female mosquitoes Response sensitivity. In addition, some experimental reports indicate that carbon dioxide (co2) has universal (not limited to specific species) attractiveness to mosquitoes, and that the attractiveness of the mosquito is proportional to the concentration of carbon dioxide. The mosquito bait in the present invention includes at least one human odor mimic to simulate human volatiles, a honey source, and a carbon dioxide supply source. The human odor mimic contains a specific bacterial decomposition product, and a culture medium using fruit juice for yeast fermentation can provide a stable source of carbon dioxide and serve as a source of honey. Experimental data The mosquitoes used in the experiment were Aegj / pi /), cultured at 23-25 ° C, relative humidity of 60-80%, and twelve-hour light / twelve-hour dark environment. Adult mosquitoes were placed in a cage covered with a curtain of 30 cm3 in size and fed with a 10% (ν / ν) sucrose aqueous solution. During the mosquito trap test experiment, the female mosquitoes were starved for 4-8 days, and then released into a dual-hole odor meter (Olfactometer). The odor meter included a flight chamber (1.6m x 0.6m x 0.6m). ) For release of mosquitoes. The air cartridge sucks fresh air through the activated carbon filter, then passes through two passes of distilled water, and releases it into the flight chamber through one of the two holes (5 cm in diameter, about 30 cm apart). An odor source is placed in another well while the temperature is maintained at 30 ° C via a water circulation system. The room temperature is maintained at 23-25t. The observation time of the mosquito trap test experiment is based on 8 paper sizes, which are in accordance with the Chinese National Standard (CNS) A4 specification (210 X 297 public love) --- τιν ------------ 丨 --Order ---- ----- Line · (Please read the precautions on the back before filling this page) 529904 6922twf.doc / 002 529904 6922twf.doc / 002 Printed by the Consumers ’Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs A7 B7 ;) Observe 15 minutes after mosquito release. Between successive experiments, each part of the odor tester was cleaned with 75% alcohol and blown with strong wind for twenty minutes to remove the odor. In addition, in order to avoid deviations caused by unknown factors related to directivity, the positions of the control and control groups were alternated between the two holes in each experiment. The bacteria used in the experiment were two strains of Coryneform bacteria (BE) and 5. // likeness (BL), respectively from the German company Sammlung von Mikrooranismen and the German company Zellkulturen GmbH and Hsinchu. Food Industry Development & Research Institute. Bacteria can be cultured in three different culture media: TSB, MB, and NB. The components of these three different culture broths are listed as follows: TSB (15 grams of tryptone + 5 grams of soy protein + 5 grams of NaCl / liter, and the pH of the aqueous solution 値 7.3); MB (50 grams of milk powder + 2.5 grams of digestive protein (Peptone) per liter, pH of aqueous solution 値 7.2); and NB (5 grams of digested protein + 1.5 grams of beef extracts) + 1.5 grams of yeast Yeast extracts + 5 grams of NaCl per liter, pH of the aqueous solution (値 7.4); the bacteria were cultured in a shake flask environment at a constant temperature of 30 ° C and 200 revolutions per minute for two days. In order to test the combination of different bacterial strains and different culture liquids, which has the highest efficiency of mosquito attraction, 4 ml of different combinations of bacterial culture liquids that have been cultivated for two days are placed in an odor measuring device and tested. As shown in Table 1, most of these strains can attract mosquitoes no matter what kind of culture medium they grow in; except for the strain BE9585, which does not attract mosquitoes. 9 The paper size applies the Chinese National Standard (CNS) A4 specification (210 X 297 mm). ) • --- ί,-— L-I —-· I l · II I- II ^ — — — — — — — — IIIII u—. (Please read the notes on the back before filling out this page) 529904 6922twf doc / 002 A7 B7 V. Description of the Invention (q) Force. Among them, only the strain BE9586 is the only strain, which shows attractiveness to female mosquitoes regardless of the combination of any three different culture broths, and culture in NB has the highest attraction to mosquitoes. In general, without heating, the attraction to mosquitoes will not be higher than 30%. Table 1. The attraction of different bacterial and culture medium combinations to adult mosquitoes
Strains TSB MB NB - - ΒΕ9585 - - - ΒΕ9586 + + +++ ΒΕ20659 NAb + ΝΑ BL20158 - ++ ΝΑ BL20425 - + ΝΑ BL20426 + + + 3該吸引率以百分比表之。”=無吸引力；“ + ” = 10% ; “++，，= 20% ;“++ +，，= 3 0% ° b NA :無法測量。 —?—*----裝—^ (請先閱讀背面之注意事項再填寫本頁) 訂---------線 經濟部智慧財產局員工消費合作社印製 爲了更進一步確認該成長於NB培養液中之菌株 BE9586的蚊蟲吸引力來源，將該菌株培養液加以離心所 得之上淸液置入氣味測量儀中加以評估。 由表二可見高達86.7%的成熟雌蚊會被前述之細菌培 養上淸液所吸引。其吸引力會比只有NB培養液之控制組 大槪高20%，比無氣味之水高25%。該實驗結果顯示從這 細菌培養上淸液所產生之易揮發物能特別地吸引成雌蚊。 10 本紙張尺度適用中國國家標準（CNS)A4規格（210 X 297公釐） 529904 6922twf. doc/002 529904 6922twf. doc/002 經濟部智慧財產局員工消費合作社印製 A7 B7 五、發明說明（名） 在這實驗中，熱度本身即顯示大約61.7%之吸引力，高於 先前實驗中未加熱之不同氣味來源。因此，由實驗結果可 推知若設有一加熱裝置，應可提供熱度來幫助揮發該細菌 分解物，提高對蚊蟲之吸引力。 表二菌株培養液離心後之上淸液置入氣味測量儀 中以評估對蚊蟲之吸引力 懸浮液a 營養液b 水c 86.7±4.2d 67.5 士 4.2 61.7士 1 1.8 3菌株培養液加以離心所得之上淸液， 離心速度爲l〇,〇〇〇xg 離心1 〇分鐘。 b細菌培養之營養液。 。爲無氣味之水溶液。 d計算方式爲（吸引之蚊蟲數/三十）再乘以百分比％ ;並重複 三次再平均之。 第1圖是依照本發明一較佳實例的一種裝置之示意 圖。請參照第1圖，該裝置100包含一上層隔間102 (Upper Compartment); —底層隔間 104 (Bottom Compartment); 以及連結兩者之一雙頭旋螺連結座106 (Thumbscrew Container)。一頂蓋108乃位於該上層隔間102之頂，由 一支撐機構支撐，例如：一有延伸壁的支撐肋，其延伸壁 可引導蚊蟲飛入導入隧孔；該頂蓋108與該上層隔間102 相隔開約1-2公分。而在該頂蓋108之下，該上層隔間102 之上側包括有複數個導入隧孔11〇,其由該上層隔間102 外側向內開，該導入隧孔110開口大的足夠讓蚊蟲進入， 本紙張尺度適用中國國家標準（CNS)A4規格（210 X 297公釐） -----L---^-----· 11-----訂---------線 (請先閱讀背面之注意事項再填寫本頁) 529904 6922twf.doc/002 經濟部智慧財產局員工消費合作社印製 A7 B7 五、發明說明（，） 而且其向內開之方式可防止進來的蚊蟲逃脫，使蚊蟲被陷 在該上層隔間102之內。 當該上層隔間102連接於該雙頭旋螺連結座106後， 置於該雙頭旋螺連結座106中間一柵格112上之一第一容 器120就會位於該上層隔間102之內部。該第一容器120 內至少包含一人體氣味模擬物和一二氧化碳供給源。該人 體氣味模擬物和二氧化碳供給源可由一酵母菌培養基提 供。若以一果汁做該酵母菌發酵的培養基，則更可提供一 蜜糖來源。 仍參考第1圖，這底層隔間104包括一可調整控制溫 度之加熱裝置114，其至少包含一時間調控機制和一溫度 調控機制。該時間調控機制可按照使用者設定之時間表來 操作調控時間，例如爲一定時器，而該溫度調控機制能按 照指示產生熱度到一指定溫度，並維持在這溫度；該溫度 調控機制譬如至少包含一金屬加熱器116，由一邏輯線路 所控制。 這可調整之加熱裝置114是經由使用者的設定，而產 生一調控之上升熱流，來幫助細菌培養和蒸發細菌分解 物。一第二容器122置於該底層隔間104內部之底層上， 該第二容器122內包含一宿主氣味模擬物，例如細菌培養 分解物。本實例中，利用特定之培養液中培養之指定微生 物（細菌）會產生新陳代謝產物或分解其培養液，而產生細 菌分解物。提供該宿主氣味模擬物（細菌分解物）的方式 包括可以採用整個細菌培養基、或只採用部分細菌培養 12 —f—^----——'---!訂---------線· (請先閱讀背面之注意事項再填寫本頁) 本紙張尺度適用中國國家標準（CNS)A4規格（210 X 297公釐） 529904 6 9 2 21 w f. d 〇 c / 0 0 2 A7 B7 五、發明說明（p) (請先閱讀背面之注意事項再填寫本頁) 液，例如只採用具強吸引力之細菌培養上淸液。本實例中， 較佳條件爲使用前述實驗所使用之桿狀菌株 (Corynebacterial strains)和培養液來提供細囷分解物；譬 如，使用菌株BE9586培養於NB培養液【5公克的消化蛋 白質+ 1.5公克的牛肉抽取物+ 1.5公克的酵母菌抽取物+5 公克的NaCl/每公升，水溶液之酸鹼度値7·4】中，以提供 該細菌生長。 該金屬加熱器Π6位於該底層隔間1〇4內部及該第二 容器122之上方，以加熱該底層隔間1〇4之內室並提供熱 度來幫助培養該第二容器122內之細菌。一廣口向下之反 向漏斗118用以聚集該加熱器116所提供之熱度，由該底 層隔間104之內部傳送至上層間隔102中，以進一步傳送 熱度去幫助酵母菌發酵。所以，該加熱器116所提供之熱 度會在二隔間中形成二層不同的熱流；一般來說，這二層 位於上層與底層隔間之不同的熱流大約分別保持在3 0 °C和 37°C。此外，這熱流除了提供熱度來培養兩種微生物和幫 助發散的引誘劑，還能模擬人體體表溫度，來進一步吸引 蚊蟲。 經濟部智慧財產局員工消費合作社印製 經由實驗顯示了結合熱度和氣味模擬物對埃及斑蚊吸 引的效果，在一長達六十分鐘之觀察，該細菌分解物配合 者熱度蒸發目自引誘筒達60%的蚊蟲’遠超過被發酵酵母菌 所引誘之44%的蚊蟲。此外，根據培養12小時之觀察， 結合細菌分解物、發酵酵母菌和熱度蒸發所達成之效果則 吸引多達85%的蚊蟲。使用細菌分解物作爲蚊蟲誘餌，可 本紙張尺度適用中國國家標準（CNS)A4規格（210 X 297公釐） 529904 ^922twf. doc/002 529904 ^922twf. doc/002 經濟部智慧財產局員工消費合作社印製 A7 B7 五、發明說明（l|) 以比其他的誘餌在一定時間內吸引到較多蚊子。 第2A圖是依照本發明一較佳實例的裝置之正面圖； 第2B圖是如第2A圖所示依照本發明一較佳實例的裝置 之部分。 請參照第2A圖，一裝置200包含一上層隔間202、 一底層隔間204和一雙頭旋螺連結座206。請參照第2B 圖，第2B圖是該裝置200之部分爆炸圖以描述其細節。 一第一容器220置於一格狀固定裝置224之上。該雙頭螺 旋連結座206至少包括一頂指螺旋226，一底指螺旋228 和一位於其間之柵格212，以分別連接該上層隔間202和 該底層隔間204。當該上層隔間202和該底層隔間204連 接對合於該該雙頭螺旋連結座206時，一第一 〇形環（O-ring) 230和一第二〇形環232分別用來密封對合處。該格狀固 定裝置224是位在該柵格212之上，因此當該上層隔間202 連接於該該雙頭螺旋連結座206後，該第一容器220即位 於該上層隔間202的內部。 該裝置200中更可包括一收集網234於該格狀固定裝 置224和這柵格212之間，以便於收集蚊蟲殘骸。該收集 網234亦可包含一黏性物質來陷捕蚊蟲，或一殺蟲劑以便 殺滅蚊蟲。再者’一通電柵格也可以加置到該上層隔間202 的內部來電斃殺滅陷捕之蚊蟲。 請參考第2B圖，該底層隔間204還包括一固定附屬 裝置236和一可移動的底拴蓋238和一第二容器222，該 固定附屬裝置236用以拴住該第二容器222，而該可移動 -------f-------.---.--訂---------線 (請先閱讀背面之注意事項再填寫本頁) 本紙張尺度翻中國國家標準（CNS)A4規格（21G X 297公爱） 529904 6922twf. doc/002 A7 _B7 五、發明說明（丨4 的底拴蓋238是可以由該固定附屬裝置236中拆離，因此 只要移開該底拴蓋238，則第二容器222可以拿出淸潔或 再裝滿。一加熱器216和一反向漏斗218是位在該固定附 屬裝置236上方，該反向漏斗218之窄口對著該柵格212 的中心，而其廣口卻向著該底層隔間204之內室，因此該 底層隔間204之內室的熱度，會經由該漏斗218之收聚而 穿越該柵格212傳送到該上層隔間202。 雖然本發明已以一較佳實例揭露如上，然其並非用以 限定本發明，任何熟習此技藝者，在不脫離本發明之精神 和範圍內，當可作各種之更動與潤飾，因此本發明之保護 範圍當視後附之申請專利範圍所界定者爲準。 1--------—訂---------線 (請先閱讀背面之注意事項再填寫本頁) 經濟部智慧財產局員工消費合作社印製 本紙張尺度適用中國國家標準（CNS)A4規格（210 X 297公釐）Strains TSB MB NB--ΒΕ9585---ΒΕ9586 + + + + ΒΕ20659 NAb + ΝΑ BL20158-++ ΝΑ BL20425-+ ΝΑ BL20426 + + + 3 The attraction rate is expressed as a percentage. "= Unattractive;" + "= 10%;" ++ ,, = 20%; "++ + ,, = 3 0% ° b NA: Not measurable. —? — * ---- 装 — ^ (Please read the precautions on the back before filling out this page) Order --------- Printed by the Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs to further confirm the mosquitoes of the strain BE9586 growing in the NB culture medium The source of attraction, centrifugal liquid obtained by centrifuging the culture solution of the strain was put into an odor measuring device for evaluation. From Table 2, it can be seen that up to 86.7% of mature female mosquitoes will be attracted by the aforementioned bacterial culture liquid. The force will be 20% higher than that of the control group with only NB culture solution, and 25% higher than that of odorless water. The results of this experiment show that the volatiles produced by the culturing solution from this bacterial culture can particularly attract adult mosquitoes. 10 This paper size applies the Chinese National Standard (CNS) A4 specification (210 X 297 mm) 529904 6922twf. Doc / 002 529904 6922twf. Doc / 002 Printed by the Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs A7 B7 ) In this experiment, the heat itself shows an appeal of about 61.7%, which is higher than before The different odor sources that were not heated during the test. Therefore, it can be inferred from the experimental results that if a heating device is provided, it should be able to provide heat to help volatilize the bacterial decomposition product and increase the attraction to mosquitoes. The mash was placed in an odor measuring instrument to evaluate the attractiveness to mosquitoes. Suspension a nutrient solution b water c 86.7 ± 4.2d 67.5 ± 4.2 61.7 ± 1 1.8 3 The bacterial solution obtained by centrifuging the culture solution of the strain was centrifuged at a speed of l Centrifuge for 10 minutes at 0,000,000 × g. B. Nutrient solution for bacterial culture... It is an odorless aqueous solution. D. The calculation method is (number of attracted mosquitoes / 30) multiplied by percentage%; Fig. 1 is a schematic diagram of a device according to a preferred embodiment of the present invention. Please refer to Fig. 1. The device 100 includes an upper compartment 102 (Upper Compartment);-a bottom compartment 104 (Bottom Compartment); and a link One of them is a double-screw container 106 (Thumbscrew Container). A top cover 108 is located on the top of the upper compartment 102 and is supported by a support mechanism, such as a support rib with an extension wall, The extension wall can guide mosquitoes to fly into the introduction tunnel; the top cover 108 is separated from the upper compartment 102 by about 1-2 cm. Under the top cover 108, the upper side of the upper compartment 102 includes a plurality of introductions. The tunnel hole 11 is opened from the outside of the upper compartment 102 inward. The introduction tunnel hole 110 is large enough to allow mosquitoes to enter. This paper size is in accordance with China National Standard (CNS) A4 (210 X 297 mm)- ---- L --- ^ ----- · 11 ----- Order --------- Line (Please read the precautions on the back before filling this page) 529904 6922twf.doc / 002 Printed by the Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs A7 B7 V. Description of the invention (,) And the way inward can prevent the mosquitoes from escaping and trap the mosquitoes in the upper compartment 102. After the upper compartment 102 is connected to the double-headed screw connection base 106, a first container 120 placed on a grid 112 in the middle of the double-headed screw connection base 106 will be located inside the upper compartment 102 . The first container 120 includes at least a human odor mimic and a carbon dioxide supply source. The human odor mimic and carbon dioxide supply source may be provided from a yeast culture medium. If a fruit juice is used as the fermentation medium for the yeast, a source of honey can be provided. Still referring to FIG. 1, the bottom compartment 104 includes a heating device 114 with adjustable temperature control, which includes at least a time regulation mechanism and a temperature regulation mechanism. The time adjustment mechanism can operate the adjustment time according to a schedule set by the user, for example, a timer, and the temperature adjustment mechanism can generate heat to a specified temperature according to instructions and maintain the temperature; the temperature adjustment mechanism, such as at least Contains a metal heater 116 controlled by a logic circuit. This adjustable heating device 114 is set by the user to generate a regulated ascending heat flow to help bacterial culture and evaporate bacterial decomposition products. A second container 122 is placed on the bottom layer inside the bottom compartment 104, and the second container 122 contains a host odor mimic, such as a bacterial culture decomposition product. In this example, the use of a specified microorganism (bacteria) cultured in a specific culture solution will produce a metabolite or decompose the culture solution to produce a bacterial decomposition product. Ways of providing the host odor mimic (bacterial decomposition product) include using the entire bacterial culture medium, or using only a portion of the bacterial culture. 12 —f — ^ ----——'---! Order ------- --Line · (Please read the precautions on the back before filling in this page) This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) 529904 6 9 2 21 w f. D oc / 0 0 2 A7 B7 V. Description of the invention (p) (Please read the precautions on the back before filling this page). For example, only use strong attractive bacteria culture liquid. In this example, the preferred conditions are to use the Corynebacterial strains and culture fluids used in the previous experiments to provide fine lysate decomposition products; for example, use strain BE9586 to culture in NB culture fluid [5 grams of digested protein + 1.5 grams Beef extract + 1.5 grams of yeast extract + 5 grams of NaCl per liter, pH of the aqueous solution 値 7.4] to provide the bacteria growth. The metal heater Π6 is located inside the bottom compartment 104 and above the second container 122 to heat the inner compartment of the bottom compartment 104 and provide heat to help cultivate bacteria in the second container 122. A wide-mouthed reverse funnel 118 is used to collect the heat provided by the heater 116 and is transferred from the inside of the bottom compartment 104 to the upper compartment 102 to further transfer the heat to help the yeast to ferment. Therefore, the heat provided by the heater 116 will form two different heat flows in the two compartments. Generally, the different heat flows of the two layers in the upper and lower compartments are maintained at about 30 ° C and 37 °, respectively. ° C. In addition, this heat flow not only provides heat to cultivate two microorganisms and attract divergent attractants, but also simulates the surface temperature of the human body to further attract mosquitoes. Printed by the Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs, the effect of combining heat and odor simulants on Aedes aegypti mosquitoes was shown through experiments. During a 60-minute observation, the heat of the bacterial decomposition product evaporates from the self-attractive tube. Up to 60% of mosquitoes' are far more than 44% of mosquitoes attracted by fermented yeast. In addition, based on the observation of 12 hours of culture, the combined effect of bacterial decomposition products, fermented yeasts and heat evaporation attracted up to 85% of mosquitoes. Using bacterial decomposition products as mosquito bait, this paper size can be applied to Chinese National Standard (CNS) A4 (210 X 297 mm) 529904 ^ 922twf. Doc / 002 529904 ^ 922twf. Doc / 002 Intellectual Property Bureau, Ministry of Economic Affairs, Consumer Consumption Cooperative Print A7 B7 5. Description of the Invention (l |) Attracts more mosquitoes in a certain period of time than other baits. Figure 2A is a front view of a device according to a preferred embodiment of the present invention; Figure 2B is a portion of the device according to a preferred embodiment of the present invention as shown in Figure 2A. Referring to FIG. 2A, a device 200 includes an upper compartment 202, a bottom compartment 204, and a double-headed screw-threaded connection base 206. Please refer to FIG. 2B, which is a partial exploded view of the device 200 to describe its details. A first container 220 is placed on a grid-shaped fixing device 224. The double-headed screw connection base 206 includes at least a top finger spiral 226, a bottom finger spiral 228, and a grid 212 therebetween to connect the upper compartment 202 and the bottom compartment 204, respectively. When the upper compartment 202 and the bottom compartment 204 are connected and mated with the double-ended spiral connection seat 206, an O-ring 230 and a second O-ring 232 are used for sealing, respectively. Injunction. The grid-shaped fixing device 224 is located above the grid 212, so when the upper compartment 202 is connected to the double-headed screw connection seat 206, the first container 220 is located inside the upper compartment 202. The device 200 may further include a collection net 234 between the grid-like fixing device 224 and the grid 212 to facilitate the collection of mosquito debris. The collection net 234 may also contain a sticky substance to trap mosquitoes, or an insecticide to kill mosquitoes. Furthermore, a power grid can also be added to the internal compartment of the upper compartment 202 to kill trapped mosquitoes. Referring to FIG. 2B, the bottom compartment 204 further includes a fixed attachment device 236, a movable bottom bolt cover 238, and a second container 222. The fixed attachment device 236 is used to fasten the second container 222, and The removable ------- f -------.---.-- order --------- line (Please read the precautions on the back before filling this page) This Paper size: Chinese national standard (CNS) A4 specification (21G X 297 public love) 529904 6922twf.doc / 002 A7 _B7 V. Description of the invention (4) The bottom bolt cover 238 can be detached from the fixed attachment 236, Therefore, as long as the bottom bolt cover 238 is removed, the second container 222 can be taken out and refilled. A heater 216 and a reverse funnel 218 are located above the fixed attachment 236, and the reverse funnel 218 The narrow opening is facing the center of the grid 212, while the wide opening is toward the inner compartment of the bottom compartment 204, so the heat of the inner compartment of the bottom compartment 204 will pass through the convergence of the funnel 218 and pass through the The grid 212 is transmitted to the upper compartment 202. Although the present invention has been disclosed above with a preferred example, it is not intended to limit the present invention. Anyone skilled in the art Without departing from the spirit and scope of the present invention, various modifications and retouching can be made, so the scope of protection of the present invention shall be determined by the scope of the attached patent application. 1 -------- —Order --------- line (Please read the precautions on the back before filling this page) Printed by the Employees' Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs This paper is sized to the Chinese National Standard (CNS) A4 (210 X 297 mm)