1246885 玖、發明說明： 曼明所屬^技術領域 本發明是有關於一種監控防治昆蟲之方法，且特別 是有關於一種利用微生物產物以吸引蚊蟲，以便於達 到控制目的或殺滅目的之方法及裝置。 先前技術 從古至今，人類一直想要有效地監控防治對人類有 害的昆蟲，例如蚊蟲。蚊蟲之所以對人類有害主要是 因爲蚊蟲會幫助散布傳染性疾病，諸如登革熱、曰本 腦炎、瘧疾和黃熱病等會經由蚊蟲而散佈傳染；因此， 爲了要防治蚊蟲相關之疾病，世界各國的公共衛生當 局均持續不斷地致力於蚊蟲防治，以消除蚊蟲相關之 疾病。除了疾病防治之原因外，蚊蟲叮咬而引致之痛 苦及不便，也是另一個殺滅蚊蟲之原因。由於完全滅 絕蚊蟲是幾乎不可能達成之目標，因此，長久以來人 類一直不斷在尋找有效防治蚊蟲之方法及機制，包括 如何吸引蚊蟲以便於捕捉及殺滅蚊蟲的方法及機制。 爲了從宿主身上吸取血液來提供卵發育所需之養 分，雌蚊會四處飛行以尋找宿主（h〇st)吸食血液；一般 而言，蚊蟲會使用其嗅覺結構偵測宿主所散發出的特 殊化學成份來鎖定宿主，在90英尺遠處即可偵測到。 而人類宿主身上散發的特殊化學成份，也就是吸引蚊 蟲之引誘劑（Attractant)，乃爲一些易揮發物。這些易 揮發物包含多達300種至400種化合物，其來源可能 1246885 是宿主之皮腺分泌物，皮膚上微生物分解產物，或兩 者皆是。 目前常見防制蚊蟲的方法，多半是經由化學方法來 減少整個蚊蟲族群的數量，或是試圖淸除它們的繁殖 棲息地。這些方法不但麻煩、效率低，而且常常是具 有生態破壞性的，特別是會引致有毒化學物至周遭環 境而造成生態污染。另外可行的方法是製造可以捕捉 蚊蟲之陷阱；其中各種不同之機制均曾用於製造蚊蟲 陷阱，而包括光線、熱、二氧化碳、八烯醇(octenol)、 水蒸氣和乳酸，也曾被考慮作爲引誘劑。 發明內容 本發明的主要目的就是在提供一種微生物媒介之誘 蚊和控制的方法及裝置，其機制包括使用一微生物產 物蚊蟲誘餌，來吸引蚊蟲，進而達到誘捕及控制蚊蟲 之目的。該誘蚊裝置中包括一可調控加熱裝置，會提 供適當的熱度幫助蚊蟲誘餌揮發及模擬人體溫度，以 及一蚊蟲誘餌發散裝置，幫助蚊蟲誘餌之發散釋放。 該蚊蟲誘餌至少包含一無菌化微生物培養（醱酵）產 物。由於該蚊蟲誘餌乃是利用人體汗液中既有的細菌 所產生的培養分解物與其代謝產物，故對於人類不具 毒性。此外，本發明也可以結合陷阱、黏性網、通電 柵格或含殺蟲劑網之使用，先吸引再捕捉蚊蟲或是先 吸引再殺滅蚊蟲，以達成防治蚊蟲目的。 依照本發明一較佳實施例，本發明之裝置可爲一固 1246885 定式或一可攜帶移動式蚊蟲控制系統，可以在室內或 在戶外使用來捕捉蚊蟲。該蚊蟲控制系統至少包括一 固定裝置以固定一容器，該容器至少包含一無菌化微 生物培養產物做爲蚊蟲誘餌。該控制系統中更可包括 一可調控的加熱裝置以提供熱源，以陷阱或黏性網來 捕捉蚊蟲，或通電柵網或殺蟲劑網來撲殺蚊蟲。 如上述之裝置及方法中更包括使用一加速蚊蟲誘餌 釋放之發散裝置，該蚊蟲誘餌發散裝置除利用加熱揮 發原理，也可以運用霧化原理，如噴霧霧化機制或是 高速震動霧化機制，而將蚊蟲誘餌以極微細之液態顆 粒，呈霧狀噴散出來，或是化學式機制以幫助吹送蚊 蟲誘餌，藉此提高該餌劑於相對空間內之揮發與擴散 速率，從而增加誘餌之有效誘蚊距離與效率。 爲讓本發明之上述和其他目的、特徵、優點能更明 顯易懂，下文特舉一較佳實施例，並配合所附圖式’ 作詳細說明如下。 實施方式 某些本來就存在於人類汗液中之細菌，已知會生產 甲硫醇（methanethiols)，而散發出如同乾酪或人類腳底 的氣味。目前已有實驗證明人類汗液對蚊蟲有頗強之 吸引力，可能是該些細菌會在人類身上引致易揮發性 引誘劑之產生。因此，若能辨認分離出這些細菌’就 可進而使用這些細菌來產生蚊蟲引誘劑，以模擬人類 氣味來引誘嗜血性蚊蟲。 .1246885 本發明中之蚊蟲誘餌包括至少一人體氣味模擬物（蚊 蟲引誘劑），以模擬人類散發之易揮發氣味。本發明中 該人體氣味模擬物至少包含一特定細菌培養（醱酵）產 物。 實驗資料 實驗使用之蚊蟲爲埃及斑蚊台灣p 東株或UGAL株），分析培養於23-25X：，60-80%之 相對濕度以及十二小時光/十二小時暗的環境。成蚊置 於大小30立方公分（cm3)罩以布幕之籠內並鎮以 10%(v/v)糖水溶液。在生物實驗（bioassay)中的ι〇_14 天大的雌蚊並沒有餵血就釋放到一具雙孔的氣味測量 儀（olfactometer)，該氣味測量儀包含一飛行室（le6m x0.6m x0.6m)以供放入蚊蟲飛行以進行測量。空氣嘲筒 吸取環境空氣經由活性碳過濾器，分別通過二瓶的蒸 餾水，而經由雙孔中之一孔（5公分直徑，兩孔分開3〇 公分左右）釋放到飛行室中。一氣味來源是置於〜孔 中，而經由一水循環系統溫度保持其溫度在3(TC。而 室溫保持在23-25°C。生物實驗之觀察時間是在放入蚊 蟲後15分鐘開始計算。在連續的各實驗之間，該氣味 測量儀之各部份都用75%之酒精來淸潔並用強風吹二 十分鐘以去除氣味。此外，爲了避免方向性等未知_ 素造成偏差，各次實驗中對照與控制組之位置是輪流 交替於雙孔之間。 實驗用之細菌爲桿狀（Coryneform)細菌之兩種，爲 12468851246885 玖, 发明说明: 曼明 belongs to the technical field of the present invention relates to a method for monitoring insects, and in particular to a method and apparatus for utilizing microbial products to attract mosquitoes for control purposes or killing purposes . Prior Art Since ancient times, humans have always wanted to effectively monitor and control insects that are harmful to humans, such as mosquitoes. Mosquitoes are harmful to humans mainly because mosquitoes help spread infectious diseases, such as dengue fever, sputum encephalitis, malaria and yellow fever, which are transmitted through mosquitoes; therefore, in order to prevent mosquito-related diseases, countries around the world Public health authorities are constantly working on mosquito control to eliminate mosquito-related diseases. In addition to the causes of disease prevention and control, the pain and inconvenience caused by mosquito bites is another cause of killing mosquitoes. Since the complete elimination of mosquitoes is almost impossible to achieve, people have long been searching for ways and mechanisms to effectively control mosquitoes, including ways and mechanisms to attract mosquitoes to capture and kill mosquitoes. In order to extract blood from the host to provide the nutrients needed for egg development, the female mosquito will fly around to find the host (h〇st) to suck the blood; in general, the mosquito will use its olfactory structure to detect the special chemistry emitted by the host. The ingredients are used to lock the host and can be detected at a distance of 90 feet. The special chemical component that is emitted from the human host, that is, the attractant that attracts mosquitoes, is some volatile matter. These volatiles contain up to 300 to 400 compounds, the source of which may be 1246885 being the host's cutaneous gland secretion, the skin's microbial decomposition products, or both. Most common methods of preventing mosquitoes are chemically reducing the number of mosquito populations or attempting to eliminate their breeding habitats. These methods are not only cumbersome, inefficient, but often ecologically destructive, especially causing toxic chemicals to the surrounding environment and causing ecological pollution. Another possible approach is to create traps that capture mosquitoes; various mechanisms have been used to make mosquito traps, including light, heat, carbon dioxide, octenol, water vapor, and lactic acid, which have also been considered Attractant. SUMMARY OF THE INVENTION A primary object of the present invention is to provide a method and apparatus for mosquito and control of microbial media, the mechanism comprising the use of a microbial product mosquito bait to attract mosquitoes, thereby achieving the purpose of trapping and controlling mosquitoes. The mosquito trap device includes a regulatable heating device that provides appropriate heat to help the mosquito bait to volatilize and simulate human body temperature, and a mosquito bait divergence device to help release the mosquito bait. The mosquito bait contains at least one sterilized microorganism culture (fermentation) product. Since the mosquito bait is a culture decomposition product and a metabolite thereof produced by bacteria existing in human sweat, it is not toxic to humans. In addition, the present invention can also be combined with traps, viscous nets, energized grids or insecticide-containing nets to attract and recapture mosquitoes or to attract mosquitoes to achieve mosquito control. In accordance with a preferred embodiment of the present invention, the apparatus of the present invention can be a solid 1246885 stereotype or a portable mobile mosquito control system that can be used indoors or outdoors to capture mosquitoes. The mosquito control system includes at least one fixture for holding a container containing at least one sterilized microbial culture product as a mosquito bait. The control system may further include a regulatable heating device to provide a heat source to trap mosquitoes with a trap or viscous mesh, or a grid or insecticide net to cull the mosquitoes. The apparatus and method as described above further comprise using a diverging device for accelerating the release of the mosquito bait. The mosquito bait diverging device can also utilize the atomization principle, such as a spray atomization mechanism or a high-speed vibration atomization mechanism, in addition to the heating and volatilization principle. The mosquito bait is sprayed out in the form of a very fine liquid particle, or a chemical mechanism to help blow the mosquito bait, thereby increasing the volatilization and diffusion rate of the bait in the relative space, thereby increasing the effective lure of the bait. Mosquito distance and efficiency. The above and other objects, features, and advantages of the present invention will become more apparent and understood by the appended claims Embodiments Some bacteria that are originally present in human sweat are known to produce methanethiols, which emit an odor similar to cheese or human soles. At present, experiments have shown that human sweat is quite attractive to mosquitoes, and it may be that these bacteria cause a volatile attractant in humans. Therefore, if these bacteria are identified, they can be used to produce mosquito attractants to mimic human odors to induce bloodthirsty mosquitoes. .1246885 The mosquito bait of the present invention comprises at least one human odor mimic (mosquito attractant) to mimic the volatile odor emitted by humans. In the present invention, the human odor mimetic contains at least one specific bacterial culture (fermentation) product. Experimental data The mosquitoes used in the experiment were A. orientalis, Taiwan, or the UGAL strain, and were cultured at 23-25X: 60-80% relative humidity and 12 hours light/twelve hours dark environment. Adult mosquitoes were placed in a cage of 30 cubic centimeters (cm3) in a curtain cage and incubated with a 10% (v/v) aqueous sugar solution. The ι〇_14-day female mosquito in the bioassay was released to a two-hole olfactometer without blood supply. The odometer contained a flight chamber (le6m x0.6m x0) .6m) for flying mosquitoes for measurement. Air Mocking The ambient air is drawn through the activated carbon filter through two bottles of distilled water and released into the flight chamber through one of the two holes (5 cm diameter, two holes separated by about 3 cm). An odor source is placed in the ~well, while the temperature is maintained at 3 (TC) via a water circulation system temperature while the room temperature is maintained at 23-25 ° C. The observation time of the biological experiment is calculated 15 minutes after the mosquito is placed. Between successive experiments, each part of the odometer was cleaned with 75% alcohol and blown with strong wind for twenty minutes to remove the odor. In addition, in order to avoid deviations caused by unknown directionality, each In the second experiment, the position of the control and control groups was alternated between the two holes. The bacteria used in the experiment were two kinds of coryneform bacteria, 1246885.
Brevibacterium epidermidis (ΒΈ)和 Β· linens (Bh) ’ 分 別從德國Sammlung von Mikrooranismen公司和德國布 魯茲維克Zellkulturen GmbH公司和位於新竹之食品工 業發展硏究所所購買。這些細菌可用三種不同的培養 液加以培養：TSB、MB和NB。這三種不同之培養液 之成份分列如下：TSB (15公克的胰化蛋白質 (tryptone)+ 5公克的大豆蛋白質（soytone)+ 5公克的 NaCl/每公升，而水溶液之酸鹼度値7.3) ; MB (50公克 的奶粉+ 2·5公克的消化蛋白質(peptone)/每公升，水溶 液之酸鹼度値7.2);和NB (5公克的消化蛋白質+ 1.5 公克的牛肉萃取+ 1.5公克的酵母菌萃取+ 5公克的 NaCl/每公升，水溶液之酸鹼度値7.4);細菌培養於恆 溫30°C，每分鐘200轉數之環境下二天之久。 爲了檢測不同的細菌株和不同培養液的組合，何者 具有最高效率的蚊蟲吸引力，取4毫升已培養兩天的 不同組合細菌培養液置入氣味偵測儀中加以試驗。 如表一所不，大部分的這些菌株，不論成長在哪一 種培養液中均能吸引蚊蟲；除了菌株BE9585對蚊蟲 沒有吸引力。其中只有菌株BE9586，不論配合任意三 種不同之培養液都有顯示蚊蟲吸引力，而且配以培養 液NB會對蚊蟲產生最高的吸引力。大致而言，若缺 少加熱裝置，則對蚊蟲的吸引力不會高於30%。 1246885 表一不同的細菌和培養液的組合對雌成蚊 之吸引力Brevibacterium epidermidis (ΒΈ) and Β· linens (Bh) ’ were purchased from Sammlung von Mikrooranismen in Germany and Zellkulturen GmbH in Germany and the Food Industry Development Research Institute in Hsinchu. These bacteria can be cultured in three different cultures: TSB, MB and NB. The components of these three different cultures are listed below: TSB (15 grams of tryptone + 5 grams of soytone + 5 grams of NaCl per liter, while the pH of the aqueous solution is 7.3); MB (50 grams of milk powder + 2·5 grams of peptone per liter, pH of the aqueous solution 値 7.2); and NB (5 grams of digested protein + 1.5 grams of beef extract + 1.5 grams of yeast extract + 5 The gram of NaCl per liter, the pH of the aqueous solution is 7.4); the bacteria are cultured at a constant temperature of 30 ° C for two days in an environment of 200 rpm. In order to detect the combination of different bacterial strains and different culture solutions, which has the highest efficiency of mosquito attraction, 4 ml of different combinations of bacterial cultures that have been cultured for two days were placed in an odor detector for testing. As shown in Table 1, most of these strains, regardless of the growth medium in which they are grown, attract mosquitoes; except for strain BE9585, which is not attractive to mosquitoes. Among them, only strain BE9586, whether combined with any three different culture liquids, shows mosquito attraction, and the culture medium NB has the highest attraction to mosquitoes. In general, if the heating device is lacking, the attraction to mosquitoes will not be higher than 30%. 1246885 Table 1 The combination of different bacteria and culture fluids for female mosquitoes
Strains TSB MB NB BE20660 +a BE9585 - ++ΑStrains TSB MB NB BE20660 +a BE9585 - ++Α
BE9586 + BE20659 NAb BL20158 - BL20425 - BL20426 + a該吸引率以百分比表之。=無吸引力； ‘‘+，，= 10% ;“++，，= 20% ;“+++，，= 30%° b NA : 無法測量。 爲了更進一步確認該成長於NB培養液中之菌株 BE9586的蚊蟲吸引力來源，將該菌株培養液加以離心 所得之上淸液置入氣味偵測儀中加以評估。 由表二可見高達86.7%的成熟雌蚊會被前述之細菌 培養上淸液所吸引。其吸引力會比只有NB培養液大 槪高20%，比無氣味之水溶液高25%。實驗結果建議 ί足這細菌培養懸浮液所產生之易揮發物能特別地吸引 雌成蚊。在實驗中，熱度本身即顯示大約61.7%之吸 弓丨力’高於先前實驗中未加熱之不同氣味來源。因此， &實驗結果可推知設有一加熱裝置，應可提供熱度來 幫助揮發該細菌分解物，提高對蚊蟲之吸引力。此外， 胃加I熱裝置更可模擬人體溫度以吸引蚊蟲。 10 1246885 表二菌株培養液加以離心所得之懸浮上淸液置 入氣味偵測儀中以評估對蚊蟲之吸引力 上淸懸浮液a 營養液b 水溶液^ 86.7±4.2d 67.5 士 4·2 61.7 士 11.8 a菌株培養液加以離心所得之懸浮液，離心速度 爲10,000xg離心10分鐘。b細菌培養之營養液。 e爲無氣味之水鎌。 ® d計算方式爲（吸引之蚊蟲數/三十)再乘以百分比 %;並重複三次再平均之。 上述實驗所採用之細菌培養（醱酵）產物之製作流程 如下。先將培養液NB [0.5%(v/w)消化蛋白質 +〇· 15%(v/w)牛肉萃取+0· 15°/〇(v/w)酵母菌萃取+5%BE9586 + BE20659 NAb BL20158 - BL20425 - BL20426 + a The attraction rate is expressed as a percentage. = unattractive; ‘‘+,,= 10%; “++,,= 20%; “+++,,= 30%° b NA : cannot be measured. In order to further confirm the mosquito attraction source of the strain BE9586 grown in the NB culture solution, the culture solution of the strain was centrifuged, and the upper sputum was placed in an odor detector for evaluation. It can be seen from Table 2 that up to 86.7% of mature female mosquitoes are attracted to the aforementioned bacterial culture sputum. Its attractiveness is 20% higher than that of the NB medium alone and 25% higher than the odorless solution. The experimental results suggest that the volatiles produced by this bacterial culture suspension can specifically attract female mosquitoes. In the experiment, the heat itself showed that about 61.7% of the suction force was higher than the different sources of odor that were not heated in the previous experiment. Therefore, the results of the experiment can be inferred that a heating device should be provided to provide heat to help volatilize the bacterial decomposition product and increase the attraction to mosquitoes. In addition, the stomach plus I heat device can simulate human body temperature to attract mosquitoes. 10 1246885 Table 2 The culture medium of the strain was centrifuged and placed in a scent detector to evaluate the attraction to mosquitoes. The suspension was a solution of nutrient solution b. 86.7±4.2d 67.5 ± 4·2 61.7 11.8 A strain of the culture solution was centrifuged to obtain a suspension which was centrifuged at 10,000 x g for 10 minutes. b nutrient solution for bacterial culture. e is an odorless water cellar. ® d is calculated as (attracted mosquitoes / thirty) and multiplied by a percentage of %; and repeated three times and averaged. The production process of the bacterial culture (fermentation) product used in the above experiment is as follows. First culture medium NB [0.5% (v / w) digested protein + 〇 · 15% (v / w) beef extract + 0 · 15 ° / 〇 (v / w) yeast extract + 5%
NaCl]於121°C、1.5大氣壓下高溫加壓20分鐘。其次 以菌株/培養液爲l:100(v/v)之比例，將菌株（〇Κ·9) φ 移入前述無菌化之培養液中培養。攪拌混合於一 2〇〇〇 升之醱酵培養器（fermentor)中進行細菌培養兩天，於 下列條件之下：初始pH 5.5、最終pH 8.5、初始O.D· 爲零、於波長600 nm下所測最終0·ϋ· = 1·9、常溫培養 溫度30°C、氣流0.2升/每分以及攪拌轉動速度1〇〇 rpm。培養醱酵之後，將其所得產物連續離心（5〇〇〇xg 之下10分鐘）以移除大部分之細菌細胞。獲得之上淸 11 I246885 液再進行高溫高壓於121°C及1.5大氣壓下20分鐘以 無菌化。然後，無菌化之上淸液再以無菌水稀釋三倍 並儲存於10 %酒精（v/w)做爲最終細菌培養（醱酵）產 物。 上述之製程描述僅爲參考之用，但是並非用以限定 本發明。實際上可視需要或成本考量，應用其他種類 之培養液或其他可行方法條件製造及處理。此外，由 於本發明之最終細菌培養（醱酵）產物已經經過進一步 處理與無菌化，故對人類及生態環境並無危害性或造 β 成污染之顧慮。 而爲進一步辨別出吸引蚊蟲之物質，使用不同之非 極性溶劑以標準萃取程序萃取，以辨識蚊蟲引誘劑之 主要有效成分。之後，以不同之非極性溶劑萃取所得 之萃取液以真空抽乾方式乾燥之，再溶於含10%酒精 水溶液中。前述之無菌化上淸液在此做爲未萃取吸引 劑，與其他組作比較。參見表三，相較於未萃取吸引 劑，不同極性之非極性溶劑萃取所得之萃取液可保留 Φ 大部分之蚊蟲吸引力。其中正己烷(極性0·1)幾乎可視 爲非極性溶劑，但正己烷萃取物對蚊蟲有最高之吸引 率。此結果指出溶於非極性溶劑的非極性揮發物是蚊 蟲引誘劑，其可能是細菌培養產物或代謝產物。而溶 劑本身對蚊蟲無吸引力。 12 1246885 表三 溶劑 極性指數 誘捕蚊蟲（％r 正己烷 0.1 61.3 二氯甲烷 3.1 52.7 醚 2.8 48.7 未萃取吸引劑 -- 64.7 水 -- 37.3NaCl was pressurized at a high temperature of 121 ° C and 1.5 atm for 20 minutes. Next, the strain (〇Κ·9) φ was transferred to the sterilized culture medium in a ratio of the strain/culture medium at a ratio of 1:100 (v/v). Stir and mix in a 2 liter fermenter for bacterial culture for two days under the following conditions: initial pH 5.5, final pH 8.5, initial OD·zero, at a wavelength of 600 nm. The final measurement was 0·ϋ·=1·9, the normal temperature culture temperature was 30 ° C, the air flow was 0.2 liter/min, and the stirring rotational speed was 1 rpm. After the fermentation was carried out, the resulting product was continuously centrifuged (10 minutes under 5 Torr x 10) to remove most of the bacterial cells. The upper 淸 11 I246885 solution was obtained and further sterilized by high temperature and high pressure at 121 ° C and 1.5 atm for 20 minutes. The sterilized top mash was then diluted three times with sterile water and stored in 10% alcohol (v/w) as the final bacterial culture (fermented) product. The above description of the process is for reference only, but is not intended to limit the invention. It can be manufactured and processed using other types of culture fluids or other feasible method conditions, depending on needs or cost. In addition, since the final bacterial culture (fermentation) product of the present invention has been further processed and sterilized, there is no concern that humans and the ecological environment are harmful or contaminated. In order to further identify the substances that attract mosquitoes, different non-polar solvents are extracted using standard extraction procedures to identify the main active ingredients of mosquito attractants. Thereafter, the extract obtained by extracting with a different non-polar solvent was dried by vacuum drying and dissolved in a 10% aqueous solution of alcohol. The aforementioned aseptic sputum is used herein as an unextracted attractant and compared to other groups. Referring to Table 3, the extraction of the non-polar solvent of different polarities retains most of the mosquito attraction compared to the unextracted attractant. Among them, n-hexane (polarity 0·1) can be regarded as a non-polar solvent, but the n-hexane extract has the highest attraction rate to mosquitoes. This result indicates that the non-polar volatiles dissolved in the non-polar solvent are mosquito attractants, which may be bacterial culture products or metabolites. The solvent itself is not attractive to mosquitoes. 12 1246885 Table 3 Solvent Polarity index trapping mosquitoes (%r n-hexane 0.1 61.3 methylene chloride 3.1 52.7 ether 2.8 48.7 unextracted attractant -- 64.7 water - 37.3
*:總共30隻雌蚊釋放使用於實驗中，共測試三次。 第1圖是依照本發明一較佳實施例的一種誘蚊裝置 之示意圖。請參照第1圖，該裝置100包含一上層隔 間 102 (Upper Compartment); —底層隔間 104 (Bottom Compartment)，而兩者以一對合機制相連接。此外， 上層隔間與底層隔間亦可以以其他已知機制連接，如 螺絲、卯釘對鎖或以緊配合機制對合。依照本發明一 較佳實施例的裝置，如第1圖，該上層隔間102可以 爲中空，而由一體成形之一上殼103所圍成。一頂蓋 籲 108乃位於該上層隔間102之頂，由一支撐機構支撐， 例如：一有延伸壁的支撐肋，其延伸壁可引導蚊蟲飛 入導入隧孔；該頂蓋108與該上層隔間102相隔開約 1-2公分。而在該頂蓋108之下，該上層隔間102之上 側更可包括有複數個導入隧孔110,其由該上層隔間 102外側向內開，該導入隧孔110開口大的足夠讓蚊 蟲進入，而且其向內開之方式可防止進來的蚊蟲逃脫， 13 1246885 使蚊蟲被陷在該上層隔間102之內。頂蓋108之上表 面可設計爲鮮明顏色以避免吸引蚊蟲停留，而頂蓋108 之下表面以及導入隧孔110之內面應爲暗色以幫助吸 引蚊蟲。 該上層隔間102更包括一捕捉滅蚊機制，例如一通 電柵網103a或一黏性網（可含殺蟲劑）103b。較佳是 使用通電柵網來殺滅被誘捕之蚊蟲。該通電柵網l〇3a 或黏性殺蟲網l〇3b，可裝置於上層隔間102之外殼103 上、位於上殼之中（設計有特定缺口區域以裝設通電 柵網或黏性殺蟲網），或是直接以通電柵網或黏性殺蟲 網取代外殼而圍出該上層隔間102。上層隔間102與 底層隔間104之間可包括一收集抽取式收集抽屜106， 收集被殺滅之蚊蟲或其碎片，以利淸除。 該底層隔間104亦爲中空，其可由一下殼105所圍 成。該下殼105可以是兩片式，或者，該下殼105也 可以是一體成形，形狀設計可爲中空具有底座而如倒 置之杯皿。而該裝置100中包含一容器120置於該底 層隔間104內部之底層上，該容器120內至少包含一 宿主氣味模擬物，例如細菌培養醱酵產物。本實施例 中，利用於特定之培養液中培養之指定微生物（細菌） 會產生新陳代謝產物或其培養液分解產物，而經過無 菌化處理的細菌培養醱酵產物。該容器120可設計爲 一瓶狀、管狀、盒狀、皿狀或一卡匣狀，視所使用細 菌培養釀酵產物之狀態而定。而該容器120可固定於 14 1246885 一固定裝置122。當該固定裝置122連接至該外殼1〇5 底座上時，該容器120即位於底層隔間1〇4之中。視 產品需要，該容器120與固定裝置122可以經由取出 置換，而便於淸潔、添加蚊蟲誘餌或置換該容器12〇。 此外，該底層隔間104更包括一電源124於其中，以 供應電力給g亥通電格網l〇3a與/或該加熱裝置114，甚 或是誘餌發散裝置。該電源124可以是交流電電源或 是以乾電池爲電源。 在此要強調的是，第1圖僅是該誘蚊裝置之示意圖，籲 該其所示之形狀、外觀與各元件之相對位置係僅供參 考之用，而非用以限制本發明之範圍。 本實施例中’較佳條件爲使用前述實驗所使用之桿 狀菌株(Corynebacterial strains)和培養液來提供細菌培 養醱酵產物；譬如，使用菌株BE9586培養於NB培養 液【5公克的消化蛋白質+ 1.5公克的牛肉萃取+ 1.5公 克的酵母菌萃取+5公克的NaCl/每公升】中，以提供 該細菌培養醱酵產物。該細菌培養醱酵產物可被處理 φ 成爲凝膠狀或各種溶液，端視環境或效率需求考量而 定。較佳情形下該細菌培養醱酵產物是呈現液體狀， 以利於配合誘餌發散裝置而加速發散。 經由實驗顯示（表二）可知，細菌培養液或水也對 蚊蟲有些許之吸引力’故亦不能排除其被使用爲蚊蟲 誘餌之可能性。 仍參考第1圖，該裝置100包括一可調整控制溫度 15 1246885 之加熱裝置114,端視裝置產品之設計而設於適當位 置，可設於上層隔間中或底層隔間中，其至少包含一 溫度調控機制和/或一時間調控機制。該時間調控機制 可按照使用者設定之時間表來操作調控時間，例如爲 一定時器，而該溫度調控機制能按照指示產生熱度到 一指定溫度，並維持在這溫度；該溫度調控機制譬如 至少包含一加熱器116，由一邏輯線路所控制。該加 熱器116較佳是置於上層隔間102之內以加強其幫助 吸引蚊蟲之能力。可調整之加熱裝置114設定產生一 · 調控之熱度，來幫助細菌醱酵產物揮發散逸或模擬人 體體溫。 此外，該裝置1〇〇中設有一蚊蟲誘餌發散裝置Π8， 以幫助蚊蟲誘餌之散逸揮發，其可視產品設計而裝設 於底層隔間之中或是上層與底層隔間之間。如第1圖 所示，，可設置於上層隔間102與底層隔間104之間、 下殻105之頂層上或配置於容器120附近。事實上， 該誘餌發散裝置118可以利用多種不同機制，而達到 φ 幫助蚊蟲誘餌散發之目的。以下將配合圖示來解釋可 應用於該誘餌發散裝置118之各種不同機制。 本發明中，如上述之裝置及方法中更包括使用一加 速蚊蟲誘餌之微粒釋放揮發裝置，該蚊蟲誘餌發散裝 置係運用霧化原理，例如以噴霧霧化機制或是以高速 震動霧化機制，而將蚊蟲誘餌以極微細之液態顆粒（約 5-50// m直徑），呈霧狀噴散出來，藉此提高該餌劑於 16 1246885 相胃空間內之揮發與擴散速率，從而增加誘餌之有效 離與效率。根據測試結果顯示，配合使用此誘 餅發散裝置後，的確使捕獲率提高至約90%。 依照〜較佳實施例，該誘餌發散裝置200係利用高 霧化機制，其可設置於上層隔間與底層隔間之 間’或下殼之上端，其裝置機制簡單示意圖如第2圖， 該誘餌發散裝置200至少包括一電路控制板（或1C晶 片）202，以控制時間、頻率及電壓，電性連結至一壓 電陶瓷片204，以提供穩定的共振頻率，例如1.9 kHz · 之頻率；而陶瓷片204連接於一振動片206，該振動 片206例如是經防銹處理之金屬薄片，其上具大小如 約5〜20/^0!的複數個孔洞210。其機制是將例如超音 波信號20傳輸到可振動的壓電陶瓷片204上，陶瓷片 快速的機械振動帶動振動片206，使其快速振動，再 傳到給振動片小孔210中的誘餌液體（位於如第1圖 之容器120內誘餌液體經由如芯心之類以毛細現象吸 導到金屬片之小孔中），由於高速振動而使得液體表面 籲 的表面張力被破壞，故使得液體霧化噴散逸出，進而 擴散至上層隔間甚或外面。 事實上，該誘餌發散裝置可視產品成本考量、使用 評估等因素，採不同機制而設置於不同位置。該誘餌 發散裝置亦可採用噴霧霧化機制或其他適當機制’而 幫助誘餌之散逸揮發。 如第3A圖所示，於本案另一較佳實施例中，該誘 17 1246885 餌發散裝置300,可位於例如底層隔間內或整合位於 該容器中。該誘餌發散裝置300至少包括一噴頭3〇2 以及一氣體吹送裝置304,其可透過套拴式機制而整 合於容器120之中，而同時蚊蟲誘餌（較佳爲液體狀） 也裝塡於於容器之中，但誘餌液體並不直接接觸（淹 沒）噴頭之頂端。該噴頭302，可利用虹吸管原理將 噴頭外部之液體狀蚊蟲誘餌導引流向該噴頭之一頂 端，而使蚊蟲誘餌以極微小液滴狀噴散逸出，形成更 易於揮發擴散之霧狀顆粒。請參見第3B圖，其爲第3A · 圖之部分放大圖。該噴頭302，例如爲一雙層不銹鋼 金屬套管所構成之噴嘴頭，而該氣體吹送裝置3〇4， 例如爲一小型空氣幫浦。該噴頭302至少包括一外管 306與一內管308。首先，利用該吹送裝置(幫浦）304 加壓打氣，將加壓之空氣通過一管路309通入該噴頭 內管308。而該噴頭外管306之底部有一小縫隙缺口 310,可利用虹吸管原理將噴嘴頭外部之蚊蟲誘餌301 導引流向該噴頭之一頂端312。通入g亥噴頭內管308 鲁 之空氣再經由噴嘴頭內管308之一頂部小孔314高速 噴出。根據應用白努力定律之噴霧原理，該噴嘴頭即 可將液體狀之蚊蟲誘餌以極微小液滴狀噴出（圖中箭 頭所示），形成更易於揮發擴散之霧狀顆粒。 而依據本發明之較佳實施例，容器120之形狀更可 進一步設計，而具有突出擋板，而阻絕大粒水珠直接 噴出，而僅容許霧狀頼粒揮發擴散。 18 1246885 前述圖中之許多裝置元件，包括電源、加熱裝置、 誘餌發散裝置之氣管及幫浦等槪念由於爲已知機制， 故在此不欲詳列其細節及運作，而其於圖示中之相對 位置，應可以因應工業設計原則，視產品外型用途需 要，而做相對調整或進一步之整合。 當然，本發明之誘餌發散裝置亦可以使用噴霧罐之 機制，利用壓差，而產生噴霧，而無須使用幫浦。 此外，本發明之誘餌發散裝置，更設計使用一種發 泡吹送(foam blowing)機制，利用特定化學物質如發泡 ® 劑（foaming agent)與水溶液進行化學反應，而產生氣體 (例如：二氧化碳）吹送誘蚊餌劑，因而加強釋放誘 蚊餌劑，強化吸引蚊蟲之效果。由於此發泡吹送機制， 不需要電源，因此特別適用於戶外使用。而當使用於 戶外之際，並可配合殺蟲劑或黏性物質，而加強滅蚊 效果。同樣地，亦可考慮添加揮發性溶劑，例如酒精， 來幫助加強釋放誘蚊餌劑。 雖然本發明已以一較佳實施例揭露如上，然其並非 0 用以限定本發明’任何熟習此技藝者，在不脫離本發 明之精神和範圍內’當可作各種之更動與潤飾，因此 本發明之保護範圍當視後附之申請專利範圍所界定者 爲準。 圖式簡單說里· 第1圖是依照本發明一較佳實施例的一種誘蚊裝置之 示意圖。 19 1246885 第2圖是依照本發明一較佳實施例的一種誘餌發散裝 置之示意圖。 第3A圖是依照本發明另一較佳實施例的一種誘餌發散 裝置之示意圖。 第3B圖是如第3A圖所示之部分放大圖。 圖式標記說明 100 :誘蚊裝置 102 ··上層隔間（Upper Compartment) 103 :上殼 103a :通電柵格 l〇3b :黏性網 104 ·•底層隔間（Bottom Compartment) 105 :下殼 106 :抽取式抽屜 108 :頂蓋 110 :導入隧孔 114 :加熱裝置 116 :加熱器 118 :誘餌發散裝置 120 :容器 122 :固定裝置 124 :電源 200、300 :誘餌發散裝置 20 1246885 202 :控制電路板 204 :陶瓷片 206 :振動片 301 :誘餌 302 :噴頭 304 :吹送裝置 306 :外管 308 :內管 309 :管路 310 :缺口 312 :頂端 314 :頂部小孔*: A total of 30 female mosquitoes were released for use in the experiment and tested a total of three times. BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a schematic illustration of a mosquito trapping device in accordance with a preferred embodiment of the present invention. Referring to Figure 1, the apparatus 100 includes an upper Compartment 102 (Bottom Compartment), and a Bottom Compartment 104, which are connected by a pair of mechanisms. In addition, the upper compartment and the bottom compartment may be connected by other known mechanisms, such as screws, dowel-to-lock or a tight fit mechanism. In accordance with a preferred embodiment of the present invention, as in Figure 1, the upper compartment 102 can be hollow and surrounded by an integrally formed upper casing 103. A top cover 108 is located at the top of the upper compartment 102 and is supported by a support mechanism, such as a support rib having an extension wall, the extension wall of which guides the mosquito to fly into the introduction tunnel; the top cover 108 and the upper layer The compartments 102 are spaced apart by about 1-2 cm. Under the top cover 108, the upper side of the upper compartment 102 may further include a plurality of introduction tunnels 110, which are opened inwardly from the outer side of the upper compartment 102, and the opening of the introduction tunnel 110 is large enough for the mosquitoes Entering, and its inward opening prevents the incoming mosquitoes from escaping, 13 1246885 causes the mosquito to be trapped within the upper compartment 102. The upper surface of the top cover 108 can be designed to be brightly colored to avoid attracting mosquitoes, while the lower surface of the top cover 108 and the inner surface of the introduction tunnel 110 should be dark to help attract mosquitoes. The upper compartment 102 further includes a capture mosquito killing mechanism, such as a grid 103a or a viscous web (which may contain insecticide) 103b. Preferably, an energized grid is used to kill trapped mosquitoes. The energized grid l〇3a or the viscous insecticidal net l〇3b may be disposed on the outer casing 103 of the upper compartment 102 and located in the upper casing (designed with a specific notch area to install a power grid or a sticky killing The insect net), or directly replaces the outer casing with a power grid or a viscous insect net to enclose the upper compartment 102. Between the upper compartment 102 and the bottom compartment 104 may include a collection and collection collection drawer 106 for collecting killed mosquitoes or fragments thereof for removal. The bottom compartment 104 is also hollow and may be enclosed by a lower casing 105. The lower case 105 may be of two pieces, or the lower case 105 may be integrally formed, and the shape may be a hollow cup having a base such as an inverted cup. The apparatus 100 includes a container 120 disposed on the bottom layer of the interior of the bottom compartment 104. The container 120 contains at least one host odor simulant, such as a bacterial culture fermentation product. In the present embodiment, the designated microorganism (bacteria) cultured in the specific culture solution generates a metabolite or a culture liquid decomposition product thereof, and the bacteria which have been subjected to the sterilization treatment culture the fermentation product. The container 120 can be designed in the form of a bottle, a tube, a box, a dish or a card, depending on the state in which the bacteria are used to culture the fermented product. The container 120 can be attached to a fixture 122 of 14 1246885. When the fixture 122 is attached to the base of the housing 1〇5, the container 120 is located in the bottom compartment 1〇4. Depending on the product requirements, the container 120 and the fixture 122 can be replaced by removal for ease of cleaning, adding a mosquito bait or replacing the container. In addition, the bottom compartment 104 further includes a power source 124 therein for supplying power to the power grid 135a and/or the heating device 114, or even the bait diverging device. The power source 124 can be an alternating current source or a dry battery. It is to be emphasized that FIG. 1 is only a schematic view of the mosquito trapping device, and the shapes, appearances, and relative positions of the components are merely for reference, and are not intended to limit the scope of the present invention. . In the present embodiment, the preferred conditions are to use the Corynebacterial strains and the culture solution used in the foregoing experiments to provide a bacterial culture fermentation product; for example, using the strain BE9586 to culture the NB medium [5 g of digested protein + 1.5 grams of beef extract + 1.5 grams of yeast extract + 5 grams of NaCl per liter to provide the bacterial culture fermentation product. The bacterial culture fermented product can be processed into a gel or a variety of solutions depending on environmental or efficiency requirements. Preferably, the bacterial culture fermented product is in a liquid form to facilitate accelerated divergence in conjunction with the bait diverging device. It has been shown by experiment (Table 2) that bacterial culture fluid or water is also somewhat attractive to mosquitoes. Therefore, the possibility of being used as a mosquito bait cannot be ruled out. Still referring to FIG. 1, the apparatus 100 includes a heating device 114 having an adjustable control temperature of 15 1246885. The end device product is designed to be located in an appropriate position and may be disposed in the upper compartment or in the lower compartment, which includes at least A temperature regulation mechanism and/or a time regulation mechanism. The time adjustment mechanism can operate the regulation time according to a schedule set by the user, for example, a timer, and the temperature regulation mechanism can generate heat according to the indication to a specified temperature and maintain the temperature; the temperature regulation mechanism is at least A heater 116 is included and is controlled by a logic circuit. The heater 116 is preferably placed within the upper compartment 102 to enhance its ability to assist in attracting mosquitoes. The adjustable heating device 114 is configured to generate a controlled heat to assist in the evaporation of the bacterial fermentation product or to simulate the body temperature of the human body. In addition, a mosquito bait diverging device 8 is provided in the device 1 to assist in the volatilization of the mosquito bait, which may be installed in the bottom compartment or between the upper and lower compartments depending on the product design. As shown in Fig. 1, it may be disposed between the upper compartment 102 and the bottom compartment 104, on the top layer of the lower casing 105, or in the vicinity of the container 120. In fact, the bait diverging device 118 can utilize a variety of different mechanisms to achieve the purpose of helping the mosquito bait to be emitted. Various different mechanisms that can be applied to the bait divergence device 118 will be explained below in conjunction with the illustrations. In the present invention, the apparatus and method as described above further comprise using a microparticle-releasing volatilization device for accelerating a mosquito bait, the mosquito decoy device utilizing an atomization principle, such as a spray atomization mechanism or a high-speed vibration atomization mechanism. The mosquito bait is sprayed out in a very fine liquid particle (about 5-50 / / m diameter), thereby increasing the volatilization and diffusion rate of the bait in the 16 1246885 gastric space, thereby increasing the bait. Effective separation and efficiency. According to the test results, the use of the biscuit divergence device did increase the capture rate to about 90%. According to a preferred embodiment, the bait diverging device 200 utilizes a high atomization mechanism, which can be disposed between the upper compartment and the bottom compartment or the upper end of the lower casing, and the device mechanism is simplified as shown in FIG. 2, The bait diverging device 200 includes at least a circuit control board (or 1C chip) 202 for controlling time, frequency and voltage, and electrically connected to a piezoelectric ceramic piece 204 to provide a stable resonant frequency, for example, a frequency of 1.9 kHz. The ceramic piece 204 is connected to a vibrating piece 206, for example, a rust-proof metal foil having a plurality of holes 210 having a size of about 5 to 20/^0!. The mechanism is to transmit, for example, the ultrasonic signal 20 to the vibrating piezoelectric ceramic piece 204. The rapid mechanical vibration of the ceramic piece drives the vibrating piece 206 to vibrate rapidly and then to the bait liquid in the vibrating piece aperture 210. (The bait liquid in the container 120 as shown in Fig. 1 is sucked into the small hole of the metal piece by a capillary phenomenon such as a core), and the surface tension of the liquid surface is broken due to high-speed vibration, so that the liquid mist is caused. The escaping escapes and spreads to the upper compartment or even the outside. In fact, the bait divergence device can be set at different locations by different mechanisms depending on factors such as product cost considerations and usage evaluation. The bait diverging device may also employ a spray atomization mechanism or other suitable mechanism to aid in the escape of the bait. As shown in Fig. 3A, in another preferred embodiment of the present invention, the lure 17 1246885 bait diverging device 300 can be located, for example, in an underlying compartment or integrated in the container. The bait diverging device 300 includes at least a nozzle 3〇2 and a gas blowing device 304, which can be integrated into the container 120 through a ferrule mechanism, while the mosquito bait (preferably liquid) is also attached to the In the container, but the bait liquid does not directly contact (submerge) the top of the nozzle. The nozzle 302 can guide the liquid-like mosquito bait outside the nozzle to the top end of the nozzle by using the siphon principle, so that the mosquito bait is sprayed and discharged in a very small droplet shape to form a mist-like particle which is more volatile and diffuses. Please refer to FIG. 3B, which is a partial enlarged view of FIG. 3A. The head 302 is, for example, a nozzle head formed of a double-layer stainless steel metal sleeve, and the gas blowing device 3〇4 is, for example, a small air pump. The showerhead 302 includes at least an outer tube 306 and an inner tube 308. First, the blowing means (pump) 304 is used to pressurize the air, and the pressurized air is introduced into the nozzle inner tube 308 through a line 309. The bottom of the nozzle outer tube 306 has a small gap notch 310, and the mosquito bait 301 outside the nozzle head can be guided to the top end 312 of the nozzle by the principle of a siphon. The air that passes into the g-head inner tube 308 is then ejected at a high speed through the top small hole 314 of the nozzle head inner tube 308. According to the principle of applying the white law of the white law, the nozzle head can eject the liquid mosquito bait in a very small droplet shape (shown by the arrow in the figure) to form a mist-like particle which is more volatile and diffuses. According to a preferred embodiment of the present invention, the shape of the container 120 can be further designed to have a protruding baffle to prevent the large water droplets from being directly ejected, and only allow the misty particles to volatilize and diffuse. 18 1246885 Many of the device components in the preceding figures, including the power supply, the heating device, the air pipe of the bait divergence device, and the pump are known as the known mechanism, so the details and operation are not detailed here. The relative position in the middle should be able to make relative adjustments or further integration according to the industrial design principles and depending on the needs of the product. Of course, the bait diverging device of the present invention can also use a spray can mechanism to generate a spray using a pressure difference without using a pump. Further, the bait diverging device of the present invention is further designed to use a foam blowing mechanism to chemically react with an aqueous solution using a specific chemical substance such as a foaming agent to generate a gas (for example, carbon dioxide) blowing. The lure bait is used to enhance the release of the bait bait and enhance the effect of attracting mosquitoes. Due to this foam blowing mechanism, no power supply is required, so it is especially suitable for outdoor use. When used outdoors, it can be combined with insecticides or viscous substances to enhance the anti-mosquito effect. Similarly, it is also conceivable to add a volatile solvent, such as alcohol, to help strengthen the release of the bait. Although the present invention has been described above in terms of a preferred embodiment, it is not intended to limit the invention, and the invention may be modified and modified without departing from the spirit and scope of the invention. The scope of the invention is defined by the scope of the appended claims. BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a schematic view of a mosquito trapping device in accordance with a preferred embodiment of the present invention. 19 1246885 Figure 2 is a schematic illustration of a bait diverging apparatus in accordance with a preferred embodiment of the present invention. Figure 3A is a schematic illustration of a bait diverging apparatus in accordance with another preferred embodiment of the present invention. Fig. 3B is a partial enlarged view as shown in Fig. 3A. Schematic description 100: mosquito trapping device 102 · upper compartment (Upper Compartment) 103: upper casing 103a: energizing grid l〇3b: viscous mesh 104 • Bottom Compartment 105: lower casing 106 : removable drawer 108 : top cover 110 : introduction tunnel 114 : heating device 116 : heater 118 : bait diverging device 120 : container 122 : fixing device 124 : power supply 200 , 300 : bait diverging device 20 1246885 202 : control circuit board 204: ceramic sheet 206: vibrating piece 301: bait 302: head 304: blowing device 306: outer tube 308: inner tube 309: tube 310: notch 312: tip 314: top hole