WO2016111478A1

WO2016111478A1 - Whitening cosmetic composition containing inorganic ring as active ingredient

Info

Publication number: WO2016111478A1
Application number: PCT/KR2015/013618
Authority: WO
Inventors: 윤석균; 노윤화; 김종광; 문성준
Original assignee: 코스맥스 주식회사
Priority date: 2015-01-06
Filing date: 2015-12-11
Publication date: 2016-07-14
Also published as: KR101791425B1; KR20160084900A

Abstract

The present invention relates to a cosmetic composition for skin whitening containing, as an active ingredient, an inorganic ring consisting of goldthread, evodia and white peony extracts. According to the present invention, it is possible to exhibit a skin whitening effect by retarding tyrosinase and inhibiting the formation of melanin in the case of a cosmetic composition containing an inorganic ring as an active ingredient.

Description

【Specification】

[Name of invention]

Cosmetic composition for whitening containing inorganic ring as active ingredient

The present invention is made by the task number 10046945 under the support of the Ministry of Knowledge Economy of Korea, the research and management institution of the project is Korea Industrial Technology Promotion Institute, the research project name is "World cl ass 300 project R & D", the research project name is "K-Cosmet ic" Development of skin cell receptor regulation functional new material and development of optimal skin delivery technology containing it ", the leading institution is Cosmax, and the research period is 2013.09.01 ~ 2017.08.31.

The present invention relates to a whitening cosmetic composition containing an inorganic ring as an active ingredient, and more particularly to a whitening cosmetic composition containing an inorganic ring consisting of extracts of sulfuric acid, sesame oil and white peony.

Background Art

The present invention relates to a mixed extract having a skin whitening function using a herbal material, and more particularly, to a mixed extract extracted by hydrothermal extraction by applying distilled water to an inorganic ring (sulphurum, sewage oil, white currant) as listed in <Consent Bogam>.

Inorganic ring is composed of extracts of Huangyan, Fructus and Earl of Peony, and in <Donggibogam>, it cures eczema (a symptom of diarrhea with black water or diarrhea with black water or reddish abdomen, not refreshing after bowel movement). It is recorded as a prescription. In addition, the research on the anti-inflammatory material called inorganic anti-inflammatory research on the RAW 264.7 cells by In-hwan Kim has already been conducted. However, previous studies have not known about the skin whitening effect of the inorganic ring, and thus, in this study, the skin whitening function of the inorganic ring revealed that it inhibits tyrosinase inhibition and melanin production in melanoma cells. . Melanin is a pigment widely distributed in animals and plants. In humans, it determines the skin color and plays an important role in protecting the human body from harmful ultraviolet rays and free radicals (Jong Eon Chin et. Al,, 2006). ). The melanin pigment is synthesized from L-tyrosine through several stages of oxidation reaction, and its biosynthesis mechanism is tyrosinase DHICA oxidase C tyrosinase related protein— 1, TRP-1), DOPAchrome tautomerase ( tyrosinase related protein I 2, TRP-2) and Catechol-0 0 methyl transferase (COMT) are involved (Arcoa P. et. al., 1993, Paval S. et. al., 1993, J imenez Cervants et. al., 1994). Among these, tyrosinase oxidizes L-tyrosine and dopa (3,4-dihydroxyphenylalanine, DOPA) to produce dopa (D0PA) and dopaquinone (D0PA-quinone). Since most of the skin color and skin pigmentation are determined by the amount and distribution of melanin in keratinocytes, do not inhibit the activity of tyrosinase that promotes the synthesis of melanin or inhibit melanin formation to test the whitening effect of whitening materials. Whether or not it matters.

To give a simple example of the melanin inhibitor currently in use,

① Hydroquinone preparation (Hydroquinone) inhibits the production of melanin pigment by inhibiting the activity of tyrosinase that is active when melanin pigment is synthesized in melanocytes. At present it is the most powerful bleaching agent.

② retinoids (ret inoid) is a synthetic derivative of vitamin A is also used in acne treatment, etc. And because of the peeling effect by changing the activity of keratinocytes, it doubles the effect of other bleaching agents.

③ Kojic acid (Koj ic acid) is less bleaching effect than hydroquinone preparation. The great advantage is that there is no irritation on the skin.

④ Vitamin C is a known fact that vitamin C is effective in whitening effect, skin regeneration effect, fine wrinkles removal. But vitamin C is very unstable. That's why very few vitamin C preparations work really well on your skin. Melanin inhibitors include hydroquinone, hydroxyanisole, ascorbic acid derivatives, kojic acid, azeraic acid, Corticosteroids, retinoids, arbutin and the like, but there are difficulties in use due to problems such as safety and economics. In particular, hydroxyanisole and hydroquinone have strong melanogenesis inhibitory activity and at the same time cause side effects such as causing denaturation or lethality of pigment cells and impairing the original function of the cells. Kojisan has problems such as discoloration during use and instability of the substance itself. As described above, a lot of preparations for skin whitening to inhibit melanin have been developed, but the use thereof is limited due to the effect of impermeability, stability on the skin, and formulation, and it is currently that the effect is not maximized by a single product. It is true. The rhubarb used in the present invention (i¾o / s chinensis or Copt is chinensis Franch var.brevisepala.T. Wang et is an evergreen perennial herb of the dicotyledonous plant of the genus Amanaceae It grows on eczema in the shade of forests in mountainous areas, the trunk is thick and extends to the side, with many beard roots, 4 ~ 5 root leaves at the end of stems. is named after. the barberry root herbal eu why because by taking the roots of barberry root that dried barberry root (黄連) in the sun, and stomach-soothing, got indigestion, abdominal pain, vomiting eu heterogeneous effects, such as anti-inflammatory, antibacterial ^■ simgye ( Prescription for the treatment of heart, heat and dysmenorrhea, sore throat pain, bleeding, and nosebleeds.

Fructus oi / s rutaecarpa or Evodia officinalis Dode) used in the present invention is an unripe fruit of the genus Fructaceae, which is an unripe fruit 0 £ Voi // a officinalis, E. rutaecarpa) ^ It is used to treat vomiting, headache, stomatitis, and toothache by controlling the circulation of the body.

Peony (白 ¾ 白, Paeonia Radix) used in the present invention is a perennial herb (/ ¾ «? / A albiflora P. and Paeonia lacti flora P.) and crops of peonies and 0¾«? / 7 / ce e / ¾ «/ obovata M.). There are 1,500 species of 35 genera and distributed all over the world, but mainly in the temperate and boreal regions of the Northern Hemisphere, Korea, Japan, China, and Sakhalin Island. Ingredients include paeoniflorin, paeonol, paeonine, tannin, benzoic acid, benzoic acid, essential oils, Resin, sugar, and the like. In oriental medicine, it is used as a medicine for retrospectives, blood sedatives, sedatives, gynecology and surgery. In the present invention, the perennial herb Peony (Paeonia lact if lora P.) was used.

Only the effects described above are known in Huang Lian, Shou Yu and Baek Luk, and even in the prior patents, there is no crawfish for the cosmetic composition for skin whitening containing inorganic ring which is extract of Hwang Ryun, Osu Yu and Baekkuk. Accordingly, the present inventors studied the skin whitening function of the inorganic ring, and as a result, the inorganic ring inhibited tyrosinase inhibitory action and melanoma production of melanoma cells.

Accordingly, the present inventors have made diligent research efforts to overcome the problems of the prior art, and as a result of investigating various natural products to develop a safe and effective cosmetic composition for skin whitening, the skin whitening containing the inorganic ring of the present invention as an active ingredient In the case of the cosmetic composition for the tyrosinase inhibitory effect and melanin formation inhibitory effect was shown, and furthermore, the inorganic ring has a tyrosinase inhibitory effect than the extract of sulfur, sesame oil and baekryeok alone, the skin whitening effect was confirmed that And completed the present invention,

[Detailed Description of the Invention]

Accordingly, the main object of the present invention is to provide a natural herbal cosmetic composition for skin whitening containing an inorganic ring having an skin whitening effect as an active ingredient by exhibiting a tyrosinase inhibitory effect and a melanin formation inhibitory effect. have.

Technical Solution

According to one aspect of the present invention, the present invention provides a cosmetic composition for skin whitening containing an inorganic ring as an active ingredient.

In addition, in the present invention, the inorganic ring is characterized in that consisting of extracts of rhubarb, sessile and earl.

The term "inorganic ring" of the present invention is composed of extracts of Huangyan, sorghum and Earl of Tooth, and <I agree Bogam> Wet the inorganic ring (swelling of the abdomen and bowel movements) It is recorded as a prescription to cure diarrhea, which is not clear afterwards, and diarrhea such as black water or diarrhea with black and red color. The present inventors have conducted research to invent a cosmetic composition for safe and effective skin whitening, and as a result, the inorganic ring exhibits a tyrosinase inhibitory effect and a melanin formation inhibiting effect, thereby confirming the skin whitening effect, and to complete the present invention. It became.

In the present invention, the extract can be extracted with any solvent conventionally used, preferably distilled water, lower alcohol having 1 to 4 carbon atoms, butylene glycol, propylene glycol, glycerin or a mixed solvent thereof It is characterized in that, more preferably, hot water extraction for 1 to 4 hours at 80 ~ 90 ^° C temperature in distilled water.

In the present invention, the mixed extract can be extracted by any extraction method used in the prior art, preferably characterized in that the hot-water extraction of the powder of sulfuric acid, sewage oil and white currant with distilled water. As an extraction method using an extraction solvent, conventional extraction methods of plants, for example, hot water extraction, reflux cooling extraction, ultrasonic extraction, supercritical extraction and the like can be used. In addition, the inorganic ring may be a mixed extract after extracting the extracts of Huang lian, sessile and Baekjak respectively, or may be an extract for the isocratic mixture of hull, sesame and Baekjak.

In the present invention, the extract is characterized in that it is contained in 0.0001 to 50% by weight relative to the total composition. In the case of 0.0001% by weight or less of the range may not be a clear whitening effect, when it exceeds 50.0 by weight ¾ there may be no obvious whitening effect increase due to the increase of the content, there is a safety problem or difficulty in preparation of the formulation There may be.

In the present invention, the composition has a skin whitening effect by inhibiting tyrosinase activity and inhibiting melanin biosynthesis. According to the experimental example of the present invention, in the case of the inorganic ring of the present invention, it was confirmed that the inhibition of tyrosinase and the formation of melanin, and more excellent in the tyrosinase inhibitory effect than the extracts of sulfur, sesame and peony alone. I could confirm that. In view of these results, the inorganic ring of the present invention effectively inhibits tyrosinase and effectively inhibits melanin production. It can be seen that it is suitable as a cosmetic composition (see Experimental Examples 1 to 5). In the present invention, the composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, masseur cream, nutrition cream, moisture cream, hand cream, foundation, essence, nutrition essence, Pack, soap, cleansing product, cleansing lotion, cleansing cream, body lotion and body cleanser characterized in that it has one formulation selected from the group consisting of.

The composition of the present invention may include other auxiliaries in addition to the carrier, for example i: cotton preservatives, antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings and the like.

Advantageous Effects

As described above, the inorganic ring of the present invention exhibits a skin whitening effect by inhibiting tyrosinase inhibition and melanin formation, and thus is suitable as a natural herbal cosmetic composition for skin whitening.

[Brief Description of Drawings]

1 is a graph showing the result of the melanin production inhibitory effect of the inorganic ring. Figure 2 is a graph showing the results for cytotoxicity of inorganic rings.

Figure 3 is a graph showing the results for the inorganic ring tyrosinase gene expression.

4 is a graph showing the results of the tyrosinase inhibitory effect as a result of the synergistic effect of the inorganic ring.

[Form for implementation of invention]

Hereinafter, the present invention will be described in more detail with reference to Examples. Since these Examples are only for illustrating the present invention, the scope of the present invention is not interpreted to be limited by these Examples. Example 1 Preparation of Inorganic Ring Extract

First, the inorganic ring material, yellow rye, sewage oil, and white peony were added to a grinder in a ratio of 1: 1, respectively, to prepare a mixed powder of 100 g, and then put into a water bath with distilled water 1000 and extracted with hot water. After extracting the hot water for 2 to 3 hours, the extract was filtered under reduced pressure with a Waterman filter paper, and then, the remaining extract was concentrated in a vacuum rotary distillation apparatus with a condenser to obtain a dry weight of 16.7 g. Experimental Example 1: Melanin production inhibition experiment using melanoma cells

Melanoma cells of B16F10 mice were cultured under defined conditions. The evaluation method is as follows. Melanoma cells were suspended in DMEM containing 10% FBS and inoculated in 6-wel l piate with lxlO ⁵ cel ls per wel l and incubated until adhered to the wel l bottom. To induce melanin production, O. luM a -MSH was treated, and then the samples were incubated for 3 days after the ^' concentration was added. The concentration range of the inorganic ring extract was set to 10 / ig / i, 25 / g /, 50 / ig / m. Arbutin 100 / zg / m was treated as a positive control of the melanin production ability experiment. The cultured cells were washed with phosphate buffered salin (PBS) and recovered with trypsin. The recovered cells were counted by a hematocytometer and collected in each treatment group so as to have the same cell number as lxlO ⁶ cel ls / mL, followed by centrifugation for 10 minutes at 10 rpm to obtain only cells. The recovered cells were dried at 60 ^° C. for 1 hour, and then 1M NaOH solution containing 10% DMS0 was added to obtain intracellular melanin. The melanin solution was measured for absorbance at 490 nm using a microplate reader to evaluate the inhibition of melanin production.

The results are shown in FIG. As shown in FIG. 1, it was observed that melanin production was reduced concentration-dependently by the inorganic ring extract. In addition, it showed melanin production inhibitory ability better than arbutin known as a whitening agent. Therefore, this experiment confirmed that the inorganic ring extract has a whitening effect. Experimental Example 2 Cytotoxicity Measurement Experiment

Melanoma cells of B16F10 mice were cultured under defined conditions. The evaluation method is as follows. Melanoma cells were suspended in DMEM containing 10% FBS per wel l Inoculate the 96-well plate with lxlO ⁴ cells and incubate until attached to the bottom of the well. Remove the medium, and add the inorganic ring extract distilled at concentrations of 0.1 / g / m £, 0.5 / g / i, 1 g / mi, 5 ug / mi, 10 fig / id, 50 ug / ml and 100 A Treated and incubated at 5% CO ₂ , 37 ^° C. for 24 hours. Afterwards, MTT (3- (4,5-dimetylthiazol-2-yl) 2,5-diphenyltetrazoliuni bromide) dissolved in DPBS was added to each well to confirm cytotoxic effects. ΜΊΤ is a pale yellow substrate that turns dark blue by respiratory chain enzymes in the mitochondria of living cells. After 4 hours, the reaction solution was discarded, and the absorbed color was measured at 570 nm using a microplate reader.

The results are shown in FIG. As shown in Figure 2, there is no decrease in cells by the inorganic ring extract. Therefore, this experiment confirmed that the inorganic ring extract is not cytotoxic. Experimental Example 3: Tyrosinase gene expression confirmation experiment

Melanoma cells of B16F10 mice were cultured under defined conditions. The evaluation method is as follows. Melanoma cells are suspended in DMEM containing 10% FBS and inoculated in 6-well plates with lxlO ⁵ cells per well and incubated until attached to the bottom of the well. To induce melanin production, treatment with O.luM α-MSH, and then take the sample. Incubated for 24 hours after addition by concentration. The concentration of the inorganic ring extract was treated with the inorganic ring extract diluted to 0.1 ug / mi, 0.5 Mg / mi, 1 ug / ml, 5 ug / mi, 10 β / mi and 50 g / i. Positive controls were treated with arbutin 100 / g / i. The cultured cells were washed with phosphate buffered saline and 1 ra trizol was collected. 1/5 ratio of 0.2 Chloroform Triz) was added and voltexed to obtain a supernatant by centrifugation at 14000 rpm for 15 minutes at 4 ^° C. 0.5 mt isopropanol was added to the supernatant and reacted for 10 minutes, followed by centrifugation at 14000 rpm for 15 minutes to obtain a precipitate. Remove the supernatant and wash twice with 200 fd 100% EtOH. The precipitate was then completely dried at room temperature and dissolved in DEPC water 20-30 ≠. RNA dissolved in DEPC was quantified and cDNA was synthesized using the same amount of RNA per group. Using SYBR GREEN master mix, a cyanine dye, as a target for synthesized cDNA Real-time polymerase chain reaction (PCR) was performed to evaluate the gene expression level of tyrosinase. The results are shown in Table 1 below.

[Table 1]

As a result, as shown in Table 1, it was confirmed that the inhibition of the expression by the inorganic ring extract in the increased expression of tyrosinase induced by a-MSH. Experimental Example 4: Tyrosinase Protein Expression Confirmation Experiment

Melanoma cells of B16F10 mice were cultured under defined conditions. The evaluation method is as follows _ᄋ culture was inoculated melanoma cells suspended in DMEM containing 10% FBS in 6-well plate with lxlO ⁵ cells per well kkaja when attached to the well bottom. To induce melanin production, O.luM α-MSH was treated, and then samples were incubated for 48 hours after the concentration was added. The concentration of the inorganic ring extract was treated with 10 /, and the positive control was treated with arbutin 100 // g /. The cultured cells were washed with phosphate buffered saline and collected in 100 ≠ RIPA lysis buffer. The suspension was voltexed and centrifuged at 14000 rpm for 20 minutes at 4 ^° C to obtain a supernatant. The amount of protein in the supernatant was quantified by Bradford assay, and SDS-PAGE electrophoresis was performed using the same amount of protein for each group. PVDF The protein band was delivered to the membrane to confirm the expression level of the protein in the ECL solution. The results are shown in FIG. As shown in FIG. 3, it was confirmed that the expression amount of tyrosinase was reduced by the inorganic ring extract treatment. Experimental Example 5: Experiment for confirming tyrosinase inhibition of inorganic rings and respective raw materials

The experimental method is the same as that of <Experiment 3>. Among them, the concentration of the inorganic ring extract was treated with 1 / ig / and the synergistic effects of the whitening effect of the inorganic ring were compared by the concentrations of 1, g / i of gold, earl pear and sewage induction. Positive controls were treated with arbutin 100 g / m £. The results are shown in Table 2 and FIG.

Table 2

As shown in Table 2 and Figure 4 it can be seen that the three medicinal herbs mixed with a higher thyrosinase inhibitory effect than each single extract.

Claims

[Range of request]

[Claim 1]

Cosmetic composition for skin whitening containing an inorganic ring as an active ingredient.

[Claim 2]

The cosmetic composition for skin whitening according to claim 1, wherein the inorganic ring is composed of extracts of yellow lotus, sesame oil, and white peach.

[Claim 3]

The cosmetic composition for skin whitening according to claim 2, wherein the extract is extracted with distilled water, lower alcohol having 1 to 4 carbon atoms, butylene glycol, propylene glycol, glycerin, or a mixed solvent thereof.

[Claim 4]

The cosmetic composition for skin whitening according to claim 2, wherein the extract is contained in an amount of 0.0001 to 50.0% by weight based on the total composition.

[Claim 5]

The cosmetic composition for skin whitening according to claim 1, wherein the composition exhibits skin whitening effect by inhibiting tyrosinase activity and inhibiting melanin biosynthesis.

[Claim 6]

According to claim 1, wherein the composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream moisturizer cream, hand cream, foundation essence, nutrition essence, pack, A whitening cosmetic composition comprising a soap, a cleansing product, a cleansing lotion, a cleansing cream, a body lotion, and a body cleanser.