WO2016076089A1

WO2016076089A1 - Liquid composition containing mannosyl erythritol lipid-b

Info

Publication number: WO2016076089A1
Application number: PCT/JP2015/079654
Authority: WO
Inventors: 知宏菅原; 周平山本
Original assignee: 東洋紡株式会社
Priority date: 2014-11-11
Filing date: 2015-10-21
Publication date: 2016-05-19
Also published as: JPWO2016076089A1; JP5930142B1

Abstract

[Problem] To provide a liquid composition which is stable in itself, has excellent stability even if added to formulations, and has improved handling properties by formulating MEL-B which has poor handling properties when in the form of an oily paste and a significantly low solubility in water or oil; and a formulation thereof. [Solution] A liquid composition containing a predetermined amount of (A) mannosyl erythritol lipid-B; (B) glycerin; (C) polyhydric alcohol (except for glycerin); (D) glycyrrhizinate; and (E) water, the liquid composition being characterized in that: the mixing mass ratio of (A) mannosyl erythritol lipid-B to (B) glycerin is 1:1-1:10; and the added mass of (C) polyhydric alcohol is less than the added mass of (B) glycerin.

Description

Liquid composition containing mannosyl erythritol lipid-B

The present invention relates to a liquid composition containing mannosyl erythritol lipid-B (MEL-B) and a formulation thereof. More specifically, the present invention relates to a composition excellent in handling property, fluidity, stock solution stability and stability at the time of dilution dispersion when MEL-B is used in cosmetic preparations and the like, and a formulation thereof. In particular, the liquid composition containing MEL-B of the present invention is a dosage form suitable for a premix product when blended in a cosmetic preparation.

The skin moisturizing function functions by binding intercellular lipids to moisture, and a skin care material having such moisturizing performance close to the original skin function is required. In recent years, consumers are increasingly interested in natural products because of concerns about safety, and materials derived from natural products, not synthetic products, are required in the skin care field. For example, materials obtained by extraction from natural products such as lecithin and saponin are used in the skin care field. In addition, research and development for using fermented products produced by microorganisms as skin care materials have been actively conducted in recent years.

The skin care material produced by the above-mentioned microorganisms is an amphiphilic substance having both a hydrophilic group and a lipophilic group, and a biosurfactant having a surface active ability. Biosurfactants, which are microorganism-derived surfactants, are being studied as environmentally advanced surfactants that are highly safe and have a low environmental impact and excellent biodegradability.

Currently, biosurfactants can be classified into five types: sugar type, acyl peptide type, phospholipid type, fatty acid type and polymer compound type. Among them, sugar type biosurfactants are the most studied, and bacteria And many types of substances produced by yeast have been reported.

As such a sugar-type biosurfactant, mannosyl erythritol lipid (hereinafter sometimes referred to as “MEL”) has recently been used in the skin care field. In MEL, as an optical isomer of erythritol having a sugar skeleton, a 4-O-β-D-mannopyranosyl-meso-erythritol structure represented by the following general formula (1) and 1-O-β-D-mannopyranosyl- There is a meso-erythritol structure (the following general formula (2)).

It has been reported that MEL having a conventional 4-O-β-D-mannopyranosyl-meso-erythritol structure has various physiological activities including antibacterial properties, antitumor properties and glycoprotein binding ability. In addition, this conventional MEL exhibits extremely unique self-assembly characteristics, and not only a slight difference in molecular structure has a great influence on the formation of self-assemblies, but also dilute solutions (6.3 × 10 ^-2 wt% or less) (Non-patent Document 1: Chem. Eur. J. 2006, Vol. 12, p2434-2440). Furthermore, a liquid crystal emulsification technique using a conventional MEL bicontinuous sponge structure (Patent Document 1: Japanese Patent Application Laid-Open No. 2007-181789) is also reported.

However, MEL is in the form of an oily paste, and has a problem that the fluidity is very low and the handling property on the preparation is poor. In addition, since it is poorly soluble in water and oil, for example, when blended into a cosmetic preparation, there are problems such as the occurrence of precipitation and poor stability of the emulsified state. .

Under such circumstances, there has been reported a technique for improving the fluidity by combining MEL and an anti-inflammatory agent, and providing a highly transparent MEL composition and its formulation (Patent Document 2: JP2011-1314A). Issue gazette). Although it seemed that the technique related to handling properties was solved by the technology, the composition mentions its own fluidity and stability over time, but this is used as a premix composition of MEL for cosmetics, etc. No mention is made of the stability over time in the final product when blended in the formulation of the preparation, that is, the stability over time of the diluted solution of the premix composition, and when the composition is actually blended into a formulation such as cosmetics, In the final product, there is a problem that stability is poor such as generation of starch and property change, and it cannot be used in actual use. Furthermore, in this document, a large amount of the pharmaceutical ingredient glycyrrhizinate that is subject to formulation restrictions in cosmetics is often required, and it was difficult to realistically formulate in cosmetic formulations (Pharmaceutical Diet Examination No. 0524001 “ Pharmaceutical ingredients that can be incorporated into cosmetics ").

JP 2007-181789 A JP 2011-1314 A

In order to widely use MEL in industry, it is very important to improve handling properties and stability in preparations. So far, there has been a report on the improvement of the handling properties of MEL by the formulation, but there is no report that mentions not only the stability of the premix composition itself, but also the stability of cosmetic preparations and the like, There has been no development of a MEL premix composition that has good stability even in the formulation and can withstand a wide range of dosage forms / formulations and can withstand actual use.

Therefore, the object of the present invention is to focus on MEL-B among MELs, and is a premix composition containing MEL-B and excellent in stability over time, and also has good stability even when blended in cosmetic preparations and the like. Then, it is providing the prescription and composition which can be mix | blended with respect to various cosmetic preparations.

As a result of intensive studies, the present inventors have found that the above problems can be solved by the following means, and have reached the present invention. That is, the present invention has the following configuration.
[1]
(A) Mannosylerythritol lipid-B, (B) glycerin, (C) polyhydric alcohol (excluding glycerin), (D) glycyrrhizinate and (E) water,
(A) The blending amount of mannosyl erythritol lipid-B is 1.0 to 25.0 mass%,
(B) The amount of glycerin is 5.0 to 70.0% by mass,
(C) The blending amount of the polyhydric alcohol is 3.0 to 30.0% by mass,
(D) The amount of glycyrrhizinate is 0.01 to 5.0% by mass,
The blending mass ratio of (A) mannosyl erythritol lipid-B and (B) glycerin is 1: 1 to 1:10, and the blending mass of (C) polyhydric alcohol is less than the blending mass of (B) glycerin. A liquid composition characterized by the above.
[2]
(C) The polyhydric alcohol is selected from the group consisting of ethylene glycol, propylene glycol, dipropylene glycol, 1,3-propanediol, 1,3-butylene glycol, 1,2-pentanediol, and 1,2-hexanediol. The composition according to [1], wherein the composition is one or more compounds.
[3]
(D) The glycyrrhizinate is one or more compounds selected from the group consisting of sodium glycyrrhizinate, sodium glycyrrhizinate and ammonium glycyrrhizinate [1] or [2] A composition according to 1.
[4]
A cosmetic composition comprising 0.01 to 10% by mass of the liquid composition according to any one of [1] to [3].

According to the present invention, an MEL-B-containing liquid composition excellent in fluidity, handling properties and stability can be provided. In particular, the present invention is excellent in stability in the state of a MEL-B-containing liquid composition, can be blended for a wide range of dosage forms and formulations, and further maintains a dissolved / dispersed state even when blended in a formulation. An object of the present invention is to provide a MEL-B-containing liquid composition suitable for practical use and a composition obtained by diluting it.

The MEL-B-containing liquid composition of the present invention is excellent in fluidity, handling properties, and stability, and is also suitable as a premixed product because it is excellent in dissolution and dispersion stability with respect to a composition obtained by diluting it. is there. “Premix product” or “premix composition” means a composition in which a functional material is easily mixed in a cosmetic preparation by previously mixing the functional material with another material. In the present invention, it refers to a composition in which MEL-B is used as a functional material, and other materials are mixed with this to improve the handling property of MEL-B and the dilution and dispersibility in cosmetic preparations.

The MEL-B-containing liquid composition provided by the present invention and a composition obtained by diluting the same have effects such as a moisturizing action, a cell activation action, an anti-aging action, a hair growth action, and a rough skin improvement action. Moreover, the improvement of the effect is anticipated by adding a various compounding component. The MEL-containing liquid composition and a composition obtained by diluting the MEL-containing liquid composition can be provided as cosmetics, quasi-drugs, medical supplies, hygiene products, and pharmaceuticals.

(Component (A) MEL-B)
The structure of MEL is shown in general formula (3). In the general formula (3), the substituent R1 is an aliphatic acyl group having 4 to 24 carbon atoms which may be the same or different. MEL is classified into four types, MEL-A, MEL-B, MEL-C, and MEL-D, based on the presence or absence of acetyl groups at the 4th and 6th positions of mannose.

Specifically, in MEL-A, in the general formula (3), the substituents R2 and R3 are both acetyl groups. In MEL-B, in the general formula (3), the substituent R2 is an acetyl group, and the substituent R3 is hydrogen. In MEL-C, in general formula (3), substituent R2 is hydrogen and substituent R3 is an acetyl group. In MEL-D, in the general formula (3), the substituents R2 and R3 are both hydrogen.

The carbon number of the substituent R1 in the MEL represented by the general formula (3) is the number of carbon atoms of fatty acids constituting triglycerides, which are oils and fats contained in the MEL production medium, and assimilation of fatty acids of the MEL-producing bacteria used. Varies depending on the degree. In addition, when the triglyceride has an unsaturated fatty acid residue, an unsaturated fatty acid residue can be included as the substituent R1 unless the MEL-producing bacterium assimilates up to the double bond portion of the unsaturated fatty acid. . As apparent from the above description, the obtained MEL is usually in the form of a mixture of compounds having different fatty acid residue portions of the substituent R1.

MEL preferably used in the liquid composition of the present invention is MEL-B having a structure represented by the general formula (4) or the general formula (5). More preferred is MEL-B having the structure represented by the general formula (5). In the general formula (5), the substituent R1 is an aliphatic acyl group having 4 to 24 carbon atoms which may be the same or different. The number of carbon atoms of the substituent R1 is not particularly limited as long as it is within the above range, but it is more preferably 8 to 14. The substituent R1 may be a saturated aliphatic acyl group or an unsaturated aliphatic acyl group, and is not particularly limited. When it has an unsaturated bond, you may have a some double bond, for example. The carbon chain may be linear or branched. In the case of an oxygen atom-containing hydrocarbon group, the number and position of oxygen atoms contained are not particularly limited.

MEL-B can be cultured and produced using microorganisms. For example, MEL-B can be isolated by purifying a MEL mixture obtained by culturing Pseudozyma antarctica, Pseudozyma sp., Etc. using silica gel column chromatography. Pseudozyma tsukubaensis is known as a bacterium that produces MEL-B, and the bacterium can also be used.

The use form of the MEL-B added to the composition of the present invention is arbitrary. For example, as MEL-B, an extract from a microorganism culture solution is used or a purified high-purity product is used. Alternatively, it may be used after being suspended in water or dissolved in a solvent. Suitable MEL-B is a purified high-purity product, and those purified to a purity of 80% to 100% are preferably used.

The present invention provides a MEL-B-containing liquid composition excellent in fluidity, handling properties and stability, but the blending amount of MEL-B does not impair the action of MEL-B as a premix product. In the MEL-B-containing liquid composition, 1.0 to 25.0% by mass is usually preferable, 3.0 to 20.0% by mass is more preferable, and 5.0 to 15.0% by mass. % Is more preferable. When the amount is less than 1.0% by mass, the advantage of the premix liquid composition is small because it is a blending mass at the cosmetic preparation level, whereas when it exceeds 25.0% by mass, the advantage is the liquid composition of the present invention. The handling property may be impaired.

(Component (B) Glycerin)
The component (B) glycerin of the present invention is a very important component for contributing to the improvement of the stability of the MEL-B-containing liquid composition and a composition obtained by diluting the MEL-B-containing liquid composition, and in particular, the component (A) MEL- The blending mass ratio of B and component (B) glycerin is important. Specifically, when the blending mass ratio of the component (A) and the component (B) is in the range of 1: 1 to 1:10, the stability becomes better, and the ratio is 1: 1 to 1: 5. More preferably, it is more preferably 1: 1 to 1: 3.

The content of (B) glycerin is not particularly limited as long as it is within the blending mass ratio of the component (A) and the component (B), but is usually preferably 5.0 to 70.0% by mass, 10.0 to 50.0% by mass is more preferable, and 15.0 to 30.0% by mass is preferable.

(Component (C) Polyhydric alcohol)
The component (C) polyhydric alcohol (excluding glycerin) of the present invention is highly compatible with the component (A), has an excellent effect as a solvent for dispersing the component (A) in water, and contains a MEL-B-containing liquid. It is very useful for improving the dispersibility of water in the composition and the dissolution stability.

Examples of the component (C) polyhydric alcohol used in the present invention include those having excellent compatibility with MEL-B as described above. For example, ethylene glycol, propylene glycol, dipropylene glycol, 1,3-propanediol, 1,3-butylene glycol, 1,2-pentanediol, 1,2-hexanediol and the like are preferably used. These polyhydric alcohols can be used alone or in combination of two or more.

The component (C) polyhydric alcohol of the present invention is excellent as a solvent for dispersing the component (A) in water, but the addition of an excessive amount may impair the storage stability of the MEL-B-containing liquid composition. is there. Therefore, it is preferable to mix | blend the compounding mass of a component (C) in the range which does not exceed the compounding mass of a component (B). The content of the (C) polyhydric alcohol is not particularly limited as long as it does not exceed the blending mass of the component (B), but is usually preferably 3.0 to 30.0% by mass, More preferred is 20.0% by mass.

The reason why the blending mass of component (C) is less than the blending mass of component (B) glycerin will be described below. Component (B) has low compatibility with component (A) MEL-B and has the effect of reducing the solubility of component (A) in water. At this time, by blending the component (C) in a range not exceeding the blending amount of the component (B), the component (A) can be efficiently dissolved in the rich condition with respect to the component (C). This state contributes not only to the storage stability of the liquid composition of the present invention, but also to the dispersion stability of the composition obtained by diluting it. If the component (C) is added in excess of the amount of the component (B), the component (A) is diluted with respect to the component (C), and the storage stability of the liquid composition containing MEL-B It will affect.

(Component (D) Glycyrrhizinate)
The component (D) glycyrrhizinate of the present invention has a very high solubility of the component (A) MEL-B, and is excellent in the effect as a component for dissolving and dispersing the component (A) in water. This is very useful for improving the dispersibility in water and dissolution stability of the contained liquid composition. However, when there is an excess amount of component (D), there is a concern that component (A) may dissolve the lamellar structure formed in the aqueous solution, so the blending mass ratio of component (A) and component (D) is very high. Is important to. That is, the blending mass ratio of the component (A) and the component (D) is preferably 5: 1 to 50: 1, more preferably 10: 1 to 40: 1, and further preferably 15: 1 to 30: 1. preferable. The content of (D) glycyrrhizic acid is not particularly limited as long as the blending mass ratio of component (A) to component (D) is within the above range, but is usually 0.01 to 1.5.0 mass. % Is preferable, 0.05 to 2.0 mass% is more preferable, and 0.1 to 1.0 mass% is preferable.

As long as the (D) glycyrrhizinate used in the present invention does not impair the solubility of the component (A), a component generally used in cosmetics may be selected. (D) As glycyrrhizinate, glycyrrhizinate sodium salt, glycyrrhizinate potassium salt, and glycyrrhizinate ammonium salt are preferable, and specifically, dipotassium glycyrrhizinate, disodium glycyrrhizinate, and monoammonium glycyrrhizinate are preferable. These glycyrrhizinates can be used alone or in combination of two or more.

(Ingredient (E) water)
The component (E) water of the present invention is used as a dissolution / dispersion medium for the components (A) to (D), but is not particularly limited as long as it is used as an external preparation for skin, for example, deionized water, distilled water. , Purified water, hot spring water, deep ocean water, steam-distilled water derived from plants such as rose water and lavender water, and the like. Among these, one kind or two or more kinds can be used in combination.

The content of the component (E) of the present invention is not particularly limited, and is appropriately determined depending on the contents of the components (A) to (D), but is generally 20.0% by mass to 80.0% by mass.

The MEL-B-containing liquid composition of the present invention can be used for normal cosmetics, quasi-drugs, pharmaceuticals, etc., in addition to the above-described components, as long as stability, fluidity, and handling properties are not impaired. Of course, various components used may be added, for example, moisturizers, thickeners, medicinal ingredients, preservatives, pigments, powders, pH adjusters, ultraviolet absorbers, antioxidants, fragrances, etc. Can be appropriately blended.

As the humectant described above, any component that is generally used in cosmetics may be used, and examples thereof include pyrrolidone carboxylic acid, lactic acid, sodium lactate, amino acid, sodium hyaluronate, and chondroitin sulfate. It is done. By adding these moisturizing agents, effects such as imparting moisturizing properties to the MEL-B-containing liquid composition of the present invention are expected.

As the above-mentioned thickener, any of ingredients generally used in cosmetics may be used, such as guar gum, quince seed, carrageenan, galactan, gum arabic, pectin, mannan, starch, xanthan gum, Curdlan, methyl cellulose, hydroxyethyl cellulose, carboxymethyl cellulose, methyl hydroxypropyl cellulose, hydroxyethyl guar gum, carboxymethyl guar gum, dextran, locust bean gum, succinoglucan, caronic acid, chitin, chitosan, carboxymethyl chitin, agar, polyvinyl alcohol, Examples include polyvinyl pyrrolidone, carboxyvinyl polymer, alkyl-modified carboxyvinyl polymer, sodium polyacrylate, bentonite and the like. These thickeners are expected to have an effect of improving the stability of the MEL-B-containing liquid composition of the present invention.

As the above-mentioned medicinal ingredients, any of ingredients generally used in cosmetics may be used. For example, vitamin A or a derivative thereof, vitamin B6 or a derivative thereof, vitamin B2 or a derivative thereof, vitamin B12, Vitamin B such as vitamin B15 or its derivatives, vitamin E such as α-tocopherol, β-tocopherol, γ-tocopherol, vitamin E acetate, vitamin D, vitamin H, pantothenic acid, panthetin, pyrroloquinoline quinone And various animal and plant extracts. By adding these medicinal ingredients, it is expected that the MEL-B-containing liquid composition of the present invention is imparted with an antioxidant action and an anti-aging effect.

As the above-mentioned preservative, any component that is generally used in cosmetics may be used, for example, parabens such as methylparaben, ethylparaben, propylparaben, butylparaben, phenoxyethanol, ethylhexylglycerin and the like. Can be mentioned. By adding these preservatives, the effect of improving the antiseptic property is expected for the MEL-B-containing liquid composition of the present invention.

As the pH adjuster, any component that is generally used in cosmetics may be used. For example, citric acid, Na citrate, lactic acid, Na lactate, Na hydroxide, K hydroxide, etc. Is mentioned. By adding these pH adjusters, effects such as improved stability are expected for the MEL-B-containing liquid composition of the present invention.

By adding various components as described above to the MEL-B-containing liquid composition of the present invention, moisturizing action, cell activation action, anti-aging action, hair growth action, rough skin improvement action, etc., depending on those ingredients It can be expected to give the effect.

Various components as described above may be embedded in the lamellar structure formed by MEL-B or / and the interior of the vesicle.

The method for preparing the MEL-B-containing liquid composition of the present invention is not particularly limited, and conventionally known methods can be used. Specifically, stirring and mixing can be performed using a propeller mixer, paddle mixer, disper mixer, homogenizer, or the like. The method for adding various components is not particularly limited, but among them, component (B) and component (D) are uniformly dissolved in component (E) and heated at 60 to 70 ° C., and then component (A) Is dissolved in component (C) and a phase heated to the same temperature is added, stirred and mixed with a homogenizer, and gradually cooled to room temperature to obtain a MEL-B-containing liquid composition. Preferred for stabilization.

The appearance of the MEL-B-containing liquid composition obtained in the present invention may be transparent, translucent, slightly turbid or white turbid, but the one exhibiting transparent to translucent is more stable and more stable. It is preferable from the viewpoint of lamella forming ability.

The MEL-B-containing liquid composition obtained as described above can be used as a premix and diluted to a cosmetic preparation such as an aqueous base or emulsion to obtain a composition different from the above. The diluent medium may be any form commonly used in cosmetics, such as hair cosmetics such as shampoos, conditioners, treatments, soaps, facial cleansers, cleansing agents, sunscreen cosmetics, packs, low viscosity Skin lotion, thick skin lotion, gel cosmetics with high viscosity, emulsified serum, milky lotion, creams and other skin cosmetics, eyeliner, mascara, lipstick, foundation makeup makeup cosmetics, bath cosmetics, etc. Can be mentioned.

When diluting the MEL-B-containing liquid composition obtained in the present invention into an aqueous substrate or emulsion, the liquid composition may be diluted alone or after being mixed with various solvents. Also good.

When the MEL-B-containing liquid composition is previously mixed with various solvents and then diluted, those having excellent compatibility with MEL-B are exemplified. Examples thereof include ethanol, ethylene glycol, propylene glycol, dipropylene glycol, 1,3-propanediol, 1,3-butylene glycol, 1,2-pentanediol, 1,2-hexanediol, glycerin and the like. These polyhydric alcohols can be used alone or in combination of two or more. By diluting after mixing with these solvents in advance, the dispersibility of the MEL-B particles in the aqueous base material can be enhanced.

The concentration of the MEL-B-containing liquid composition in the cosmetic preparation is not particularly limited as long as it does not impair the dispersion stability of MEL-B upon dilution, but the final concentration of the liquid composition is not limited. The concentration is usually preferably from 0.01 to 10% by weight, more preferably from 0.1 to 5% by weight, even more preferably from 1 to 3% by weight, based on the cosmetic preparation. By blending in these ranges, it is possible to impart a feeling of use peculiar to MEL-B to the cosmetic, a moisturizing action based on a lamellar structure, and the like.

The appearance of the composition obtained by dilution may be transparent, translucent, slightly turbid or cloudy, and the physical properties may be liquid, gel, cream, or solid.

As a special case, for a lotion preparation containing about 1% by mass of a liquid composition containing MEL-B, a thickening polysaccharide such as sodium hyaluronate and a salt component such as sodium chloride are added in an amount of 0.1 to 0. When mixed in an amount of 5% by mass, the solubility of MEL-B, which is highly dispersed in water, rarely decreases, and MEL-B may precipitate. A MEL-B-containing lotion preparation is obtained by solubilizing MEL-B by mixing and dissolving a certain type of thickener in the lotion preparation on which MEL-B is deposited. be able to. Here, as the thickener, a thickener having a hydrophobic chain added to the hydrophilic polymer can be used. For example, an (acrylates / alkyl acrylate (C10-30)) crosspolymer (Acrylates / C10- 30 Alkyl Acrylate Crosspolymer), stearoxyhydroxypropyl methylcellulose (Hydroxypropyl Methylcellulose Stearoxy Ether), (PEG-240 / decyltetradeceth-20 / HDI) copolymer (PEG-240 / HDI Copolymer Bis-Decyltetradeceth-20 Ether) A powerful thickener can be mentioned. By blending these thickeners in a small amount of 0.01 to 0.5% by mass, the effect of solubilizing MEL-B can be exhibited.

When the MEL-B-containing liquid composition obtained by the present invention is diluted, the dispersibility and stability of MEL-B in the dilution medium is very good and prevents sedimentation of MEL-B particles. It is not necessary to study difficult prescriptions. Therefore, it is very easy to mix the MEL-B-containing liquid composition into the cosmetic preparation. Further, not only the skin effect of MEL-B but also a moist and good feeling of use can be imparted to the cosmetic preparation, so it is very useful as a premix product.

The MEL-B-containing liquid composition of the present invention and a composition obtained by diluting the same are widely used when a moisturizing effect, a cell activation effect, an anti-aging effect, a hair-growth effect, a skin roughening effect, etc. are required. Can do. The amount to be used is determined within a range in which the desired moisturizing effect, cell activation effect, anti-aging effect, hair-growth effect, and rough skin improving effect are sufficiently exhibited.

The MEL-B-containing liquid composition provided by the present invention and a composition obtained by diluting the same have cleaning properties, moisturizing action, cell activation action, anti-aging action, hair growth action, skin roughening action, etc. It is also useful as a component for cosmetics.

Since the MEL-B-containing liquid composition of the present invention and a composition obtained by diluting the same are used with the expectation of the above-mentioned effects, they are of course used as cosmetic compositions and cosmetics. In addition, in the form of a quasi-drug composition, medical product composition, sanitary product composition, pharmaceutical composition, functional food composition, quasi-drug, medical product, hygiene product, pharmaceutical product, etc. Can be used.

The following examples further illustrate the present invention in detail but are not to be construed to limit the scope thereof.

(Production example)
Production Example 1 Production of MEL-B P.tsukubaensis was cultured with aeration and stirring in a YM medium at a culture temperature of 26 ° C. for 48 hours using a 500 ml Sakaguchi flask. The obtained solution is used as a seed culture solution. The obtained seed culture solution was cultured with aeration and agitation in a YM medium (containing 5% olive oil) at a culture temperature of 26 ° C. for 7 days using a 10 L jar fermenter. An equal amount of ethyl acetate was added to the culture and stirred for distribution. An appropriate amount of anhydrous sodium sulfate was added to the ethyl acetate layer and allowed to stand for 30 minutes, followed by heating and concentration to obtain crude MEL-B. The obtained crude MEL-B was eluted with hexane: acetone = 5: 1 and hexane: acetone = 1: 1 using a silica gel column. Only the MEL-B fraction was collected and concentrated to obtain purified MEL-B.

(Example 1-11, Comparative example 1-11)
Liquid compositions of Examples and Comparative Examples were prepared with the components and blending ratios shown in Tables 1 to 3.

(Conditions for preparing the liquid composition)
MEL-B and polyhydric alcohol are mixed and dissolved at the same temperature in an aqueous solution in which glycerin and glycyrrhizinate are mixed and dissolved in water and kept at 60 to 70 ° C. at the mixing ratio shown in the following table. The solution was added little by little and stirred vigorously with a homomixer at the same temperature. The liquid composition was obtained by continuing this, cooling to normal temperature. As the polyhydric alcohol, ethylene glycol, propylene glycol, dipropylene glycol, 1,3-propanediol, 1,3-butylene glycol, 1,2-pentanediol and 1,2-hexanediol were used. As glycyrrhizinate, sodium glycyrrhizinate, potassium glycyrrhizinate and ammonium glycyrrhizinate were used.

(Evaluation methods)
(Evaluation of storage stability of liquid composition)
In order to evaluate the storage stability of the obtained liquid composition itself, each liquid composition was stored in a thermostatic bath at 5 ° C. and 40 ° C. for 2 months (corresponding to a year of room temperature based on the general concept of cosmetics). Thereafter, the sample was taken out, allowed to equilibrate to 25 ° C., evaluated for properties, judged according to the following criteria, and the results were also shown in Tables 1 to 3 (Result 1 in the table).
The properties were evaluated by visually observing the degree of transparency and the degree of separation with a 5 ° C. stored product as a standard. This evaluation means the storage stability evaluation of the stock solution when the liquid composition is used as a premix preparation. (No change in properties is observed for products stored at 5 ° C.)
[Judgment] (Result 1. Storage stability of liquid composition)
A: Compared with a 5 ° C. standard product, almost no change in properties is observed.
B: Some change is seen in transparency and color tone, but they are not separated.
C: The property changes and a slightly separated phase is observed.
D: Separated and clearly different in properties.

(Evaluation of dispersion stability of diluted solution)
In order to evaluate the dispersion stability of the diluted liquid composition obtained, each liquid composition was diluted 100 times with purified water, and the transmittance was measured (result 2 in the table). In addition, after storing each diluted solution in a thermostatic bath at 5 ° C. for 2 months, by measuring the transmittance again, the dispersibility of the diluted solution was evaluated and judged according to the following criteria, and the results are shown in Tables 1 to 3. It was written together (result 3 in the table). Further, the transmittance of the diluted solution after preparation and after storage at low temperature was compared, and the property change was also determined according to the following criteria, and the results were also shown in Tables 1 to 3 (Result 4 in the table).
The transmittance was measured using purified water as a control, the measurement object was placed in a 10 mm long cell, and the transmittance at a wavelength of 660 nm was measured using a Hitachi spectrophotometer (U-1900).
This evaluation is based on the knowledge that the solubility of MEL-B in water decreases at low temperatures, and as an accelerated test, it was stored at 5 ° C. to evaluate the precipitation and aggregation of dispersed MEL-B particles. Evaluation is made with respect to dispersibility when the liquid composition is diluted. The evaluation of the dispersibility of the diluted solution means the evaluation of the storage stability when the liquid composition is blended as a premix preparation into an aqueous preparation such as lotion.
[Determination] (Dispersibility of 2.100-fold diluted solution (during preparation))
(3. Dispersibility of 100-fold diluted solution (5 ° C, 2 months))
A: Transmittance 100 to 80%: Very high transparency and highly dispersed B: Transmittance 60 to 80%: Highly transparent and dispersed C: Transmittance 40 to 60%: Slightly transparent Low, poor dispersibility D: Transmittance 0-40%: opaque, dispersion is temporary
[Judgment] (4. Properties of 100-fold diluted solution (5 ° C, 2 months))
A: There is no change in properties and no precipitation is observed.
B: Transparency is slightly changed, but no precipitation is observed.
C: Transparency decreases and slight precipitation is observed.
D: A large amount of precipitation is clearly observed.

From Examples 1 to 4, by changing the other components and their ratios appropriately in accordance with the change in the amount of MEL-B, a liquid composition obtained and a composition with good stability of the diluted liquid can be obtained. It was. (See Table 1)

It is known that the degree of solubility with MEL-B varies depending on the type of polyhydric alcohol. From Examples 5 to 11, when changing the type of polyhydric alcohol to be blended, the blending amount was appropriately adjusted to obtain a liquid composition to be obtained and a composition having good stability of the diluted liquid. . (See Table 2)

From Comparative Examples 1 to 4, when glycerin, polyhydric alcohol, glycyrrhizinate, and purified water were not added, the storage stability of the obtained liquid composition or the dispersibility of the diluted solution was poor.

From Comparative Examples 5 and 6, when the blending mass ratio of MEL-B: glycerin deviated from 1: 1 to 1:10, the storage stability of the resulting liquid composition and the dispersibility of the diluted solution were poor.

From Comparative Example 7, when the blended mass of the polyhydric alcohol exceeded the blended mass of glycerin, the storage stability of the resulting liquid composition was particularly poor.

From Comparative Examples 8 and 9, when polyhydric alcohol and glycerin were not blended, remarkable dispersion failure of the diluent was observed over time.

From Comparative Examples 10 and 11, when glycerin is not blended and a polyhydric alcohol and a large amount of glycyrrhizinate are blended, the properties of the resulting liquid composition are maintained, but a remarkable dispersion failure of the diluted solution is observed over time. Observed.

According to the present invention, it is possible to provide a MEL-B premix formulation that has not only storage stability but also good dispersibility when diluted. With this liquid composition and its formulation, a very useful cosmetic preparation can be prepared, and it is expected to greatly contribute to the industry.

Claims

(A) Mannosylerythritol lipid-B, (B) glycerin, (C) polyhydric alcohol (excluding glycerin), (D) glycyrrhizinate and (E) water,
(A) The blending amount of mannosyl erythritol lipid-B is 1.0 to 25.0 mass%,
(B) The amount of glycerin is 5.0 to 70.0% by mass,
(C) The blending amount of the polyhydric alcohol is 3.0 to 30.0% by mass,
(D) The amount of glycyrrhizinate is 0.01 to 5.0% by mass,
The blending mass ratio of (A) mannosyl erythritol lipid-B and (B) glycerin is 1: 1 to 1:10, and the blending mass of (C) polyhydric alcohol is less than the blending mass of (B) glycerin. A liquid composition characterized by the above.
(C) The polyhydric alcohol is selected from the group consisting of ethylene glycol, propylene glycol, dipropylene glycol, 1,3-propanediol, 1,3-butylene glycol, 1,2-pentanediol, and 1,2-hexanediol. The liquid composition according to claim 1, wherein the liquid composition is one or more compounds.
(D) The glycyrrhizinate is one or more compounds selected from the group consisting of sodium glycyrrhizinate, potassium glycyrrhizinate and ammonium glycyrrhizinate. Liquid composition.
A cosmetic preparation comprising 0.01 to 10% by mass of the liquid composition according to any one of claims 1 to 3.