WO2016064358A1 - Procédé de transformation de substances organiques, et activateur biologique - Google Patents

Procédé de transformation de substances organiques, et activateur biologique Download PDF

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Publication number
WO2016064358A1
WO2016064358A1 PCT/TR2015/000330 TR2015000330W WO2016064358A1 WO 2016064358 A1 WO2016064358 A1 WO 2016064358A1 TR 2015000330 W TR2015000330 W TR 2015000330W WO 2016064358 A1 WO2016064358 A1 WO 2016064358A1
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WO
WIPO (PCT)
Prior art keywords
microorganisms
species
geobacillus
microorganism
bacillus
Prior art date
Application number
PCT/TR2015/000330
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English (en)
Inventor
Mevlut ALDIRMAZ
Original Assignee
Bi̇o Ha Us Bi̇yoteknoloji̇ Maki̇na Paz. Ltd. Şti.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
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Application filed by Bi̇o Ha Us Bi̇yoteknoloji̇ Maki̇na Paz. Ltd. Şti. filed Critical Bi̇o Ha Us Bi̇yoteknoloji̇ Maki̇na Paz. Ltd. Şti.
Publication of WO2016064358A1 publication Critical patent/WO2016064358A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/141—Feedstock
    • Y02P20/145—Feedstock the feedstock being materials of biological origin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Definitions

  • the present invention relates to a method for providing the conversion of organic substances, in particular the organic wastes, preferably for obtaining feed raw material and/or organic fertilizer (compost), and to a bio-activator used for same.
  • the object of the invention is to provide an effective microorganism activator for conversion of organic substances.
  • Another object of the invention is to provide an effective method for organic substance conversion.
  • the activator of the invention basically comprises of a mixture of bacteria, fungus and enzyme.
  • the invention relates to microorganism activator suitable for organic substance conversion, and comprises of at least one Geobacillus sp. species of microorganisms, at least one Bacillus sp. species of microorganisms, at least one Pseudomonas sp. species of microorganisms, at least one Bifidobacterium sp. species of microorganisms, at least one Lactobacillus sp. species of microorganisms; at least one Thermoascus sp. species of microorganisms, at least one Sporotrichum sp. species of microorganisms and at least one Chaetomium sp. species of microorganisms, and an enzyme.
  • the activator comprises at least two Geobacillus sp. species of microorganisms.
  • the activator further comprises of at least one Streptomyces sp. species of microorganisms, at least one Corynebacterium sp. species of microorganisms and at least one Talaromyces sp. species of microorganisms.
  • the enzyme of the activator is preferably obtained from at least one Thermoascus sp. species of microorganisms, at least one Sporotrichum sp. species of microorganisms and Talaromyces sp. species of microorganisms.
  • the invention relates to a method for preparation of a microorganism activator suitable for use in the conversion of a organic substance and comprises of the following steps:
  • Geobacillus sp. species of microorganisms at least one Geobacillus sp. species of microorganisms, at least one Bacillus sp. species of microorganisms, at least one Pseudomonas sp. species of microorganisms, at least one Bifidobacterium sp. species of microorganisms, at least one Lactobacillus sp. species of microorganisms; at least one Thermoascus sp. species of microorganisms, at least one Sporotrichum sp. species of microorganisms and at least one Chaetomium sp.
  • microorganisms and an enzyme, - adding an enzyme comprising at least one Thermoascus sp. species of microorganisms, at least one Sporotrichum sp. species of microorganisms and Talaromyces sp. species of microorganisms to the aforementioned microorganism mixture, - leaving the obtained microorganism and enzyme mixture to mature,
  • the method for preparation of the activator further contains the step of mixing vegetal and/or animal powder mixture.
  • the invention relates to a procedure for the conversion of the organic substances, wherein the method comprises the step of contacting the organic substances with the aforementioned activator for a period in the range of 6 to 48 hours at a temperature range of 50 to 100 C°.
  • the activator used for converting the organic substances is a mixture of bacteria, fungus and enzyme, wherein the bacteria and fungus, especially the thermophilic ones, that ensure self-aerobic decomposition of the organic substances (organic wastes) in the nature are first isolated and reproduced and activated in-vitro and then stabilized after addition to the wastes at a rate of 1/10.000 - 1/100, preferably at the order of 1/1000, and then mixing in a mixer for a period of 6 - 48 hours, preferably for 24 hours.
  • the bacteria used are bacteria of aerobic thermophilic species.
  • the activator comprises at least one Geobacillus sp. species of microorganisms, at least one Bacillus sp. species of microorganisms, at least one Pseudomonas sp. species of microorganisms, at least one Bifidobacterium sp. species of microorganisms, and at least one Lactobacillus sp. species of microorganisms as bacteria microorganism.
  • the activator may also comprise at least one Streptomyces sp. species of microorganisms and at least one Corynebacterium sp. species of microorganisms as bacteria microorganism.
  • the activator may comprise microorganisms selected from a group consisting of Streptomyces pactum as Streptomyces sp. species; Corynebacterium striatum as Corynebacterium sp. species; and Bacillus pumilis, Bacillus stearotermophilus, Bacillus cereus, Bacillus brevis, Bacillus suptilis, Bacillus sphearieus, Bacillus licheniformis, Bacillus cardotenax, Bacillus pallidus and Bacillus smith as Bacillus sp.
  • microorganisms selected from a group consisting of Geobacillus thermodenitrificans, Geobacillus subterraneus, Geobacillus anatolicus, Geobacillus stearotermophilus, Geobacillus uralicus, Geobacillus uzenensis, Geobacillus gargaensis, Geobacillus lituanicus, Geobacillus sacchari, Geobacillus thermoleovorans, Geobacillus kaustophilus, Geobacillus thermoglucosidasius, Geobacillus toebii, Geobacillus caldoxylosilyticus as Geobacillus sp. species.
  • the activator comprises at least two Geobacillus sp. species of microorganisms and these microorganisms are preferably Geobacillus stearotermophilus and Geobacillus thermodenitrificans species.
  • the activator comprises at least one Thermoascus sp. species of microorganisms, at least one Sporotrichum sp. species of microorganisms and at least one Chaetomium sp. species of microorganisms.
  • the activator may comprise microorganisms selected from a group that includes Thermoascus aurantiacus, Thermoascus thermophilus and Thermoascus aegyptiacus as Thermoascus sp. turu,; Sporotrichum thermophile as Sporotrichum sp. species; and Chaetomium thermophile microorganisms as Chaetomium sp. species out of the fungus microorganisms.
  • microorganisms selected from a group that includes Thermoascus aurantiacus, Thermoascus thermophilus and Thermoascus aegyptiacus as Thermoascus sp. turu,; Sporotrichum thermophile as Sporotrichum sp. species; and Chaetomium thermophile microorganisms as Chaetomium s
  • the activator may also comprise a group of microorganisms selected from Thermoas cuscrustaceus, Thermomyces ibadanensis, Thermomyces lanuginosus, Thermomyces stellatus, Melanocarpus thermophilus, Rhizomucor pusillus, Mucor miehei, Torula thermophila, Thielavia thermophila, Chaetomium thermophile, Stibella thermophila, Myceliophthora thermophila, Choetomium mesoptamicum, Choetomium thermophilum, Choetomium thermophilevar, Canariomyces thermophila, Mariococcum thermophilum, Myricoccum thermophilum, Paecilomyces varioti, Stillbella thermophila, Talaromyces byssochlamydioides, Talaromyces emersonii, Talaromyces thermophilus, Penicillium pinophilum, Sporot
  • the enzyme content of the activator can be obtained from any, or a multitude of microorganisms subject to the invention, but preferably the species Sporothchum thermophile, Talaromyces emersonii and Thermoascus aurantiacus are used.
  • Sporothchum thermophile is incubated under aerobic conditions at approximately 50 C° using PDA (Potato Dextrose Agar) medium and the enzymes are obtained through continuous or batch cultures in the fermenter.
  • Talaromyces emersonii is incubated under aerobic conditions at approximately 50 C° using MEA (Malt Extract Agar) medium, and the enzymes are obtained through continuous or batch cultures in the fermenter.
  • Thermoascus aurantiacus is incubated under aerobic conditions at approximately 50 C° using Emerson YpSs Agar medium, and the enzymes are obtained through continuous or batch cultures in the fermenter.
  • the enzymes obtained from the microorganisms are digestive enzymes such as proteases, lipases, a-amylase, glucoamylase, cellulase, hemicellulase, pectinase, ligninase, cellobiozdehydrogenase, xylanase, a-D-glucuronidase, polygalacturonase, laccase, lactase, phytase, D-galactosyltransferase, trehalase, ⁇ - glucosidase, lipoamiddehydrogenase, ATP sulphurilase, protein disulfidisomerase.
  • digestive enzymes such as proteases, lipases, a-amylase, glucoamylase, cellulase, hemicellulase, pectinase, ligninase, cellobiozdehydrogenase
  • the activator contains aerobic thermophilic bacteria at the rate of preferably 1 x 10 6 to 1 x 10 12 in 1 g activator and such quantity is more preferably in the range of around 1 x 10 8 to 1 x 10 10 . It is preferable to have even amount of species to be used in the bacteria mixture.
  • the amount of fungus mixed in the activator is preferably 1 x 10 6 to 1 x 10 12 in 1 g activator, and such quantity is more preferably in the range of around 1 x 10 8 to 1 x 10 10 . It is preferable to have even amount of species to be used in the fungus mixture.
  • the bacteria used in the activator of the invention are preferably reproduced in- vitro as known in the prior art. Therefore, the bacteria species is incubated for 2 days under aerobic conditions at a certain temperature (such temperature might vary depending on the species of the bacteria, e.g.
  • the fungi used in the activator of the invention are also preferably reproduced in- vitro as known in the prior art. Therefore, the fungi species is incubated for 2 days under aerobic conditions at a temperature of 45 C° using suitable medium (for instance, RFA (Rabbit Food Agar) for Thermoascus thermophilus, PDA (Potato Dextrose Agar) for Sporotrichum thermophile, PCA (Potato Carrot Agar) for Chaetomium thermophile).
  • suitable medium for instance, RFA (Rabbit Food Agar) for Thermoascus thermophilus, PDA (Potato Dextrose Agar) for Sporotrichum thermophile, PCA (Potato Carrot Agar) for Chaetomium thermophile.
  • the bacteria and fungus cultures reproduced are prepared and mixed in quantities set forth above.
  • the enzyme solution is added to the microorganism mixture via fermenter or batch culture in such manner to have minimum 1 x 10 9 unit of enzyme per liter.
  • the microorganism mixture and enzyme solution preferably mixed at equal amounts is then left for maturing in the ambient temperature for approximately 1 day.
  • the activator mixture is then mixed with preferably a vegetal and/or animal powder mixture in order to ensure long- term stability and the ambient humidity is removed through, preferably, ventilation.
  • the powdered product of any plant such as powdered lentil, chickpea, beans, rice, soy, sugar might be used as vegetal powder, but preferably lentil and rice flour is used.
  • the powdered product of any animal product such as fish flour, poultry flour, blood flour, and bone flour might be used, but preferably fish flour and poultry flour are used.
  • the powder mixture used preferably comprises of animal and vegetal powders mixed at equal amounts. Such powder mixture is then agitated in such manner to have 1 liter of microorganism - enzyme mixture per kg of the powder mixture.
  • Such mixture is then dehumidified by applying ventilation through supply of hot or cold air as known in the prior art and then agitation until the humidity drops down to and below 10% (or dried naturally by laying on a smooth concrete floor as a thin layer), thus activator is obtained by culturing the microorganism - enzyme mixture completely on the powdered material.
  • the activator is preferably mixed with an organic substance or waste for a duration of 6 - 48 hours, preferably for 24 hours, and at a temperature in the range of 50 to 100 C°, preferably in the range of 70 to 80 C°, at an organic substance : activator rate of 1/10.000 - 1/100, preferably at the rate of approximately 1/1000, by mass in order to convert the organic substances.
  • the mixing operation is performed with a suitable mixer known in the art.
  • the process and outcome parameters related to the decomposition of various organic substances using an activator of the invention are provided hereunder for illustrative purposes:
  • the studies set forth hereunder are performed under constant monitoring with computers of the organic substances used in all studies and the process within a continuously operating mixer fitted with air fans for ventilation and heated with hot oil. During such studies, the organic substances are aerated for 24 hours, ensuring aerobic decomposition at temperature in the range of 70-80 C°. 10 kg activator is used in all studies.
  • Such activator contained a microbial mixture comprising of 25% fish flour, 25% lentil flour, 25% poultry flour, 24% rice flour and approximately 1 % fungus (Sporotrichum thermophile and Chaetomium thermophile) and bacteria (Geobacillus stearotermophilus and Geobacillus thermodenitrificans) species and the enzymes obtained from Sporotrichum thermophile, Talaromyces emersonii, Thermoascus aurantiacus using a fermenter.
  • fungus Sporotrichum thermophile and Chaetomium thermophile
  • bacteria Gaobacillus stearotermophilus and Geobacillus thermodenitrificans
  • Organic substance 9 tons of poultry fertilizer, 3 tons of fungus compost waste, 3 tons of slaughter house waste
  • Organic substance 4 ton at fertilizer, 4 tons of slaughter house waste, 7 tons of biogas decanter waste
  • Raw material mixture 4 tons of poultry fertilizer, 3 tons of fungus compost waste,
  • Organic substance 3 tons of poultry fertilizer, 3 ton at fertilizer, 3 tons of fungus compost waste, 6 tons of slaughter house waste
  • Organic substance 6 tons of goat fertilizer, 6 tons of thyme waste, 3 tons of whey Input humidity: 54%

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fertilizers (AREA)
  • Treatment Of Sludge (AREA)

Abstract

La présente invention concerne un procédé de transformation de substances organiques, en particulier de déchets organiques, de préférence pour obtenir des matières premières alimentaires et/ou des engrais organiques (compost), et un bio-activateur utilisé à cet effet, comprenant au moins une espèce Geobacillus sp., au moins une espèce Bacillus sp., au moins une espèce Pseudomonas sp., au moins une espèce Bifidobacterium sp. et au moins une espèce Lactobacillus sp. de micro-organismes; des microorganismes fongiques comprenant au moins une espèce Thermoascus sp., au moins une espèce Sporotrichum sp. et au moins une espèce Chaetomium sp. des micro-organismes et une enzyme.
PCT/TR2015/000330 2014-10-24 2015-10-26 Procédé de transformation de substances organiques, et activateur biologique WO2016064358A1 (fr)

Applications Claiming Priority (2)

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TR2014/12525 2014-10-24
TR201412525 2014-10-24

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106978366A (zh) * 2017-03-24 2017-07-25 广西大学 一种混合菌剂及其在促进堆肥腐熟中的应用
CN107446866A (zh) * 2017-09-27 2017-12-08 福建省农科农业发展有限公司 一种异位发酵处理粪污的复合微生物菌剂
CN108299087A (zh) * 2018-02-11 2018-07-20 平南县德湖种养农民专业合作社 一种龟背竹有机肥料及其制作方法
CN110184196A (zh) * 2019-05-09 2019-08-30 慕恩（广州）生物科技有限公司 一种篮状菌及其应用
CN111808784A (zh) * 2020-08-04 2020-10-23 中国海洋大学 一株产耐高温α-淀粉酶的热反硝化地芽孢杆菌Y62及其应用
CN111808779A (zh) * 2020-07-29 2020-10-23 中国科学院上海高等研究院 一种嗜热地芽孢杆菌及其在农业废弃物中的应用
CN112851446A (zh) * 2021-01-28 2021-05-28 于佳骏 一种提升普洱茶树的大叶产量的叶面肥及其制备方法
CN114480215A (zh) * 2022-03-08 2022-05-13 中国科学院上海高等研究院 一种复合微生物菌剂及其在沼渣高温好氧堆肥中的应用
EP4215511A1 (fr) * 2022-01-25 2023-07-26 Eco-Habitat B.V. Procédé de décomposition bactérienne de déchets organiques

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GB2478929A (en) * 2010-03-23 2011-09-28 Biomax Technologies Pte Ltd Treatment of organic waste with microorganisms
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EP2179978A2 (fr) * 2008-10-27 2010-04-28 André Gilhofer L'invention concerne un procédé de fermentation régulée de boues d'épuration, à l'aide desquelles les pertes d'azote, la viscosité et les émissions d'odeurs peuvent être réduites
GB2478929A (en) * 2010-03-23 2011-09-28 Biomax Technologies Pte Ltd Treatment of organic waste with microorganisms
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Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106978366A (zh) * 2017-03-24 2017-07-25 广西大学 一种混合菌剂及其在促进堆肥腐熟中的应用
CN106978366B (zh) * 2017-03-24 2020-07-28 广西大学 一种混合菌剂及其在促进堆肥腐熟中的应用
CN107446866A (zh) * 2017-09-27 2017-12-08 福建省农科农业发展有限公司 一种异位发酵处理粪污的复合微生物菌剂
CN108299087A (zh) * 2018-02-11 2018-07-20 平南县德湖种养农民专业合作社 一种龟背竹有机肥料及其制作方法
CN110184196A (zh) * 2019-05-09 2019-08-30 慕恩（广州）生物科技有限公司 一种篮状菌及其应用
CN111808779A (zh) * 2020-07-29 2020-10-23 中国科学院上海高等研究院 一种嗜热地芽孢杆菌及其在农业废弃物中的应用
CN111808779B (zh) * 2020-07-29 2022-07-05 中国科学院上海高等研究院 一种嗜热地芽孢杆菌及其在农业废弃物中的应用
CN111808784A (zh) * 2020-08-04 2020-10-23 中国海洋大学 一株产耐高温α-淀粉酶的热反硝化地芽孢杆菌Y62及其应用
CN111808784B (zh) * 2020-08-04 2021-06-08 中国海洋大学 一株产耐高温α-淀粉酶的热反硝化地芽孢杆菌Y62及其应用
CN112851446A (zh) * 2021-01-28 2021-05-28 于佳骏 一种提升普洱茶树的大叶产量的叶面肥及其制备方法
EP4215511A1 (fr) * 2022-01-25 2023-07-26 Eco-Habitat B.V. Procédé de décomposition bactérienne de déchets organiques
NL2030681B1 (en) * 2022-01-25 2023-08-04 Eco Habitat B V Process to bacterially decompose organic waste material
CN114480215A (zh) * 2022-03-08 2022-05-13 中国科学院上海高等研究院 一种复合微生物菌剂及其在沼渣高温好氧堆肥中的应用

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