WO2016060462A1 - Acaricide naturel contre les acariens, marqueur d'identification pour acariens et leur procédé de fabrication - Google Patents

Acaricide naturel contre les acariens, marqueur d'identification pour acariens et leur procédé de fabrication Download PDF

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WO2016060462A1
WO2016060462A1 PCT/KR2015/010828 KR2015010828W WO2016060462A1 WO 2016060462 A1 WO2016060462 A1 WO 2016060462A1 KR 2015010828 W KR2015010828 W KR 2015010828W WO 2016060462 A1 WO2016060462 A1 WO 2016060462A1
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formula
mites
mite
ticks
alkyl group
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PCT/KR2015/010828
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English (en)
Korean (ko)
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이회선
양지연
송자은
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전북대학교산학협력단
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Priority claimed from KR1020140140038A external-priority patent/KR101661961B1/ko
Priority claimed from KR1020150134926A external-priority patent/KR101787831B1/ko
Application filed by 전북대학교산학협력단 filed Critical 전북대학교산학협력단
Priority to CN201580056103.2A priority Critical patent/CN107072198A/zh
Publication of WO2016060462A1 publication Critical patent/WO2016060462A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

  • the present invention relates to a natural acaricide and / or identification marker for ticks using a compound fractionated and purified from Melissa officinalis (lemon balm) essential oil or paleo extract.
  • Sanitary pests are distributed around human life and are always considered to be a great pain.
  • human diseases caused by infectious disease-borne pests are difficult to completely eliminate these pests due to the dynamical link between human and the pest. Therefore, in the field of hygienic pests, there is a great interest in the development of effective control methods for these infectious pests.
  • Allergic diseases that occur worldwide are caused by lifestyles, and in modern indoor dwellings, the incidence rate is also increasing due to the increased frequency of exposure of allergens to the air.
  • Airborne allergens include mites, animal hair and pollen, and these antigens are known to be closely related to the development of allergies. Once exposed to these antigens, research reports show an increased incidence of rhinitis, asthma and atopic dermatitis. Mites are the major mediating antigens that cause allergic diseases, and the predominant species are Dermatophagoides farinae , D. pteronyssinus , and Tyrophagus putrescentiae .
  • mite control methods using chemicals such as benzyl benzoate or DEET (diethyl- m- toluamide) are mainly used.
  • Such chemical control has to be treated periodically because of the short duration of treatment, which causes the appearance of resistant individuals and remnants in the environment due to repeated treatment.
  • allergens remain on the controlled tick body, making it virtually impossible to remove.
  • the killer mite which has become a problem recently, is a small mite mite ( Haemophysalis longicorni ) of the True Mite family , which is mainly found throughout Korea in May-August.
  • small cattle tick is a mediated pest of severe febrile thrombocytopenia syndrome virus (SFTSV) with a mortality rate of around 10%, and the proportion of small cattle tick infected with severe thrombocytopenia syndrome virus is 0.5%.
  • SFTSV severe febrile thrombocytopenia syndrome virus
  • house dust mites large-legged dust mites / vertical pattern dust mites
  • stored food mites and small cattle mites
  • acaricides that have the function of spraying house dust mites are applied to stored food mites or small cattle mites
  • Many papers have been published that do not have a function. Therefore, there is a need for the development of natural acaricides having acaricide function for various types of ticks.
  • An object of the present invention is to provide a natural acaricide for ticks of biomaterials and derived compounds having acaricide activity against ticks such as house dust mites and small cattle mites that act as antigens of allergic and severe febrile thrombocytopenia syndrome.
  • the present invention is to provide a tick identification indicator showing the display activity by inducing a color change of the tick.
  • the present invention relates to a natural acaricide for ticks, and provides a natural acaricide for ticks comprising a compound represented by the following formula (1) or formula (2) as an active ingredient.
  • R 1 is a C 1 ⁇ C 3 linear alkyl group, C 3 ⁇ C 5 Crushed alkyl group or hydrogen
  • R 2 is a C 1 ⁇ C 3 linear alkyl group, C 3 ⁇ C 5 A branched alkyl group or hydrogen
  • a, b, and c may each be a single bond or a double bond.
  • R 1 is a straight chain alkyl group of C1 to C5 or C3 to C5 crushed alkyl group
  • R 2 is -OH
  • n and m satisfy 0 ⁇ n + m ⁇ 4
  • n is an integer of 0-4
  • m is an integer of 0-3.
  • R 1 of Formula 1 is hydrogen or a straight alkyl group of C 1 ⁇ C 2
  • R 2 of Formula 1 is hydrogen or a straight alkyl group of C 1 ⁇ C 2
  • R 1 may be a C 1 to C 2 linear alkyl group
  • n may be an integer of 0 to 3
  • m may be an integer of 0 to 2.
  • R 1 of Formula 1 may be hydrogen or a methyl group (CH 3 ), R 2 of Formula 1 may be a hydrogen or methyl group (CH 3 ).
  • the natural acaricide for ticks of the present invention is 3,7-dimethyl-2,6-octadiene represented by the following formula (3), octanal represented by the formula (4), 2-jade represented by the formula (5)
  • Tenal, 2,4-octadieneal represented by the formula (6) and 3,7-dimethyl-6-octenal represented by the formula (7) may be any one selected from.
  • the compound represented by Formula 1 and Formula 3 to 7 is characterized in that it is derived from the fractions of the essential oil extract of Melissa of ( Melissa officinalis , lemon balm), the compound represented by Formula 2
  • the silver may be derived from the fraction purified product of the extract.
  • the natural acaricide for the mite dust mites may have a potency to kill one or more ticks selected from the group consisting of.
  • it can be characterized in particular for its ability to kill a small small mite ( Haemaphysalis longicornis ).
  • the present invention includes any one or more selected from the compounds represented by the following formulas (3), (5), (6) and (8) as an active ingredient, and provides an tick indicator for ticks, characterized in that it has a tick killing effect.
  • R 1 is a C 1 ⁇ C 5 linear alkyl group or C 3 ⁇ C 5 Crushed alkyl group, n is an integer of 2 to 3.
  • the identification marker is a large leg mite ( Dermatophagoides farinae ), vertical pattern mite ( Dermatophagoides pteronyssinus ), long hair mite ( Tyroglyphus) putrescentiae ) and stored food ticks ( Tyrophagus putrescentiae ) is characterized in that the identification effect for any one or more ticks selected from the group consisting of.
  • the present invention is to perform a fraction purification process of the concentrated Melissa essential oil or pulverulent extract by silica gel chromatography and high-performance liquid chromatography to prepare a fractional tablet, the fractional tablet comprises a compound represented by the formula (1) or (2) It provides a method for producing a natural acaricide for ticks.
  • the present invention provides a tick killing and labeling composition
  • a tick killing and labeling composition comprising the Melissa (Melissa officinalis, lemon balm) essential oil or rupture extract as an active ingredient.
  • Natural acaricide according to the present invention is not a conventional chemical control agent, but a natural acaricide, a natural acaricide having an active ingredient derived from Melissa essential oil or rupture extract has an excellent acaricide activity against ticks such as house dust mite and small cattle mite In addition, it also has a labeling activity that can be easily identified by the naked eye by inducing discoloration of ticks. Therefore, after the house dust mite, which is a major allergen, is removed, the discolored tick carcass can be removed to prevent secondary infection by these.
  • 1 is an optical micrograph of a tick killed by a conventional tick acaricide (DEET) and Melissa (lemon balm) essential oil.
  • FIG. 2 is an optical microscope photograph of a mite (large leg dust mite, vertical pattern dust mite) fertilized with diethyl-m-toluamide (DEET), which is a chemical control agent for the conventional house dust mite.
  • mite large leg dust mite, vertical pattern dust mite
  • DEET diethyl-m-toluamide
  • FIG. 3 is an optical microscope photograph of a mite (large-legged dust mite, vertical patterned dust mite) fertilized with acaricide of the present invention.
  • C 1 means carbon number.
  • C 2 and the like used in the present invention mean carbon number.
  • C 1 -C 3 alkyl group means an alkyl group having 1 to 3 carbon atoms.
  • R 1 is independently a hydrogen atom, a methyl group or an ethyl group, and a is 1 to 3 ".
  • a 3 R 1 substituents.
  • Each of the plurality of R 1 are the same as or different from each other, each of the R 1 may be a hydrogen atom, a methyl group or an ethyl group, or each of the R 1 is different, one of R 1 Hydrogen atom, the other is methyl group and the other is ethyl group.
  • the above content is an example of interpreting the substituents represented in the present invention, and other types of similar substituents should be interpreted in the same manner.
  • Natural acaricide for ticks refers to a drug used for killing ticks.
  • the major acaricides for ticks include organic phosphorus insecticides such as tetraethyl pyrophosphate, shuradan, demethone, and methyl demethone, DN agent, chlorobenzylate, kelsen, fluoroacetamide and sodium selenite.
  • Natural acaricide for ticks of the present invention may be characterized by comprising a compound represented by the following formula (1) or (2).
  • R 1 is a C 1 ⁇ C 3 linear alkyl group, C 3 ⁇ C 5 Crushed alkyl group or hydrogen
  • R 2 is a C 1 ⁇ C 3 linear alkyl group, C 3 ⁇ C 5 It is a branched alkyl group or hydrogen
  • said a, b, and c are a single bond or a double bond, respectively.
  • R 1 may be hydrogen or a straight alkyl group of C 1 to C 2
  • R 2 may be hydrogen or a straight alkyl group of C 1 to C 2
  • R 1 may be hydrogen or methyl group (CH 3 )
  • R 2 may be hydrogen or methyl group (CH 3 ).
  • the natural acaricide component for ticks of the present invention is 3,7-dimethyl-2,6-octadiene represented by the following formula (3), octanal represented by the formula (4), 2-octenal represented by the formula (5), formula (6) It may be any one selected from the group consisting of 2,4-octadiene and 3,7-dimethyl-6-octenal represented by the formula (7).
  • R 1 is a C 1 ⁇ C 5 linear alkyl group or C 3 ⁇ C 5 Crushed alkyl group, preferably a C 1 ⁇ C 2 linear alkyl group, more preferably a methyl group to be.
  • R 2 is -OH.
  • n and m satisfy 0 ⁇ n + m ⁇ 4, preferably 2 ⁇ n + m ⁇ 3, wherein n is an integer of 0 to 4, preferably 0 to 3 Is an integer.
  • m is an integer of 0 to 3, preferably an integer of 0 to 2.
  • the compound represented by the formula (1) may include one or more selected from, and preferably , , , , , , , , , And It may include one or more selected from, more preferably , , , , , And It may include one or more selected from.
  • Compounds represented by the formula (1), (5) and (6) of the present invention may be derived from the fractional purification of Melissa ( Melissa officinalis, lemon balm) essential oil extract, the compound represented by the formula (8) is from the fractional purification of the rupture extract It may be derived.
  • the natural acaricide for ticks provided by the present invention can be used for all ticks, but preferably, dusty mites ( Dermatophagoides) farinae ), vertical dust mite ( Dermatophagoides) pteronyssinus), food storage mites (Tyrophagus putrescentiae), dust mites ginteol (Tyroglyphus putrescentiae ) and Haemaphysalis longicornis ( Haemaphysalis longicornis ) has a potency to kill any one or more ticks selected from the group consisting of, among them is characterized in that the ability to kill, especially small cattle ticks.
  • the tick a potency of the essential oil of Melissa appeared to be the most potent of the dust mites and small cattle mites among the various tick types (see Table 2), using the contact method Melissa's essential oils showed the highest ability to kill small cattle ticks (see Table 3).
  • the natural acaricide for ticks of the present invention can be used in one dosage form selected from liquids, emulsions, coatings, fumigants, fumigants, granules and solids, preferably in one dosage form selected from solutions, coatings and fumigants. .
  • the present invention can be used as an identification marker of the tick, as well as the natural acaricide for ticks described above, in the case of the identification marker, the compound represented by the following formula (3), (5), (6) and (8) as an active ingredient can do.
  • R 1 is a C 1 ⁇ C 5 linear alkyl group or C 3 ⁇ C 5 Crushed alkyl group, preferably a C 1 ⁇ C 2 linear alkyl group, more preferably a methyl group to be.
  • N is an integer of 2 to 3, preferably an integer of 2.
  • the identification marker component of the present invention is 3,7-dimethyl-2,6-octadiene represented by the following formula (3), 2-octenal represented by the formula (5), and 2,4-octadiene all represented by the formula (6). It may be any one selected from the group consisting of.
  • Chemical Formula 8 Chemical Formula 8 is Can be.
  • tick indicator for ticks of the present invention while the tick in contact with the identification indicator, the color change occurs, it is changed to black, dark gray or yellow.
  • the discoloration effect when the compound is treated with a tick is shown in Table 5 of the present invention and FIGS. 1 to 3.
  • Compounds represented by Formula 3, Formula 5 and Formula 6 of the present invention may be derived from a fraction of purified Melissa extract ( Melissa officinalis , lemon balm) essential oil extract, wherein the compound represented by Formula 8 fractions rupture extract Purified purified product.
  • the identification markers of the present invention all have an identification effect with respect to ticks, and preferably a large dust mite ( Dermatophagoides farinae ), Vertical dust mite ( Dermatophagoides pteronyssinus ), Long hair mite ( Tyroglyphus putrescentiae ) And stored food mite ( Tyrophagus putrescentiae There is an identification effect on any one or more ticks selected from the group consisting of
  • small cattle tick Haemaphysalis longicornis
  • small cattle tick Haemaphysalis longicornis
  • the present invention also provides a tick killing and labeling composition
  • a tick killing and labeling composition comprising the Melissa (Melissa officinalis, lemon balm) essential oil or rupture extract as an active ingredient.
  • the compound of the present invention in the group treated with DEET control group did not have a color, but when treated with Melissa essential oils experimental group appeared to have a yellow color, the compound of the present invention can more easily identify the tick It was confirmed that it can function as a marker (see FIGS. 1 to 3).
  • Melissa essential oil or rupture powder and 1 step of preparing an extract using a mixture of water hexane as the extraction solvent; Drying and extracting the extract to separate the extraction solvent and the essential oil, and then preparing a concentrated extract concentrated under reduced pressure; Performing three fractionation purification of the concentrated extract through silica gel chromatography to obtain eight fractionation layers; A four step of re-acquiring five fractionation layers by fractional purification of a fractionation layer component having acaricide activity against ticks among the eight fractionation layers through silica gel chromatography; Re-acquiring three fractions of the fractionation layer component having acaricide activity against ticks from the five fractionation layers reacquired by high-performance liquid chromatography; And a step of assaying the fractional layer component for acaricide activity against ticks of the three fractionated layers obtained by reacquisition, and obtaining fractional purified product of the extract; and performing a process comprising a natural acaricide for ticks You can do
  • the rupture powder of step 1 may be prepared by washing the rupture sufficiently with distilled water, drying and pulverizing the rupture.
  • the first step is performed by using a water vapor distillation extraction apparatus to perform an extraction process for 5 to 7 hours at 75 ° C. to 85 ° C., preferably at 5:30 to 6 hours 30 minutes under 78 ° C. to 83 ° C. Extracts can be prepared.
  • the Melissa essential oil of the first step may be prepared by extracting the Melissa starch using distilled water, and continuously extracted under the following extraction conditions. (Extraction temperature, 75 °C; extraction time, 5 hours; extraction solvent, hexane ( hexane); extraction volume, 200 ml).
  • the reduced pressure concentration of the second step may be carried out under 30 °C ⁇ 40 °C, preferably 32 °C ⁇ 38 °C, wherein, if the reduced pressure concentration temperature is less than 30 °C too low pressure concentration can not be performed There may be a problem causing the denaturation of the active ingredient in the extract above 40 °C.
  • fractional purification through silica gel chromatography in steps 3 and 4 may be performed using hexane and an ethyl acetate mixed organic solvent.
  • fractional purification through high performance liquid chromatography in step 5 may be performed using methanol as an organic solvent.
  • balm ( Lemon balm A) essential oil extraction
  • the Melissa outficinalis outpost used in the present invention was washed with distilled water, dried and ground.
  • the pulverized Melissa outpost (100 g) and 1,200 ml of distilled water were injected into a 3,000 ml round bottom flask and continuously extracted using steam distillation extraction (SDE).
  • Extraction conditions used in the present invention are as follows (extraction temperature, 75 °C; extraction time, 5 hours; extraction solvent, hexane; extraction capacity, 200 ml).
  • the Melissa extract was stored in refrigerated by concentrating under reduced pressure after removing the Melissa essential oil and impurities using anhydrous magnesium sulfate, filter paper and a vacuum concentrator.
  • Morinda officinalis was washed three times in 1,000 ml of distilled water, and then pulverized by drying for at least 48 hours in a shaded and ventilated place.
  • 150 g of the pulverized sample was injected with 1,500 ml of distilled water and extracted at 80 ° C. for 6 hours using a steam distillation extractor. At this time, 300 ml of hexane was used as an extraction solvent.
  • the extract was dried over anhydrous magnesium sulfate, separated from the extraction solvent and the essential oil using a filter paper, concentrated under reduced pressure at 35 °C and sealed and refrigerated.
  • the acaricide active compound included in the extract of Paju extracted in Example 1 was fractionally purified.
  • Silica gel chromatography and high-performance liquid chromatography were performed to fractionally purify the active ingredient of the break against the tick.
  • a mixed organic solvent of hexane and ethyl acetate was used in a stepwise ratio as an eluting solvent, and fractionated 5 ml per minute to obtain eight fractionated layers (MO1 to MO8).
  • MO1 to MO8 fractionated layers
  • the acaricide activity was excellent in the MO3 fraction layer, and hexane and ethyl acetate were mixed in the ratio of 8: 2 from the MO3 fraction layer.
  • the acaricidal assay of the present invention was carried out by using the fumigation method and the contact method, the acaricidal assay for large leg mites, vertical pattern mites, stored food mites and small cattle mites.
  • the initial dose was determined based on the preliminary experiment, and 50 ⁇ l of the initial dose (104 ⁇ g / cm 2 ) of the sample was diluted, treated with filter paper, and dried at room temperature for 20 minutes. Only 50 ⁇ l of acetone was treated as a negative control.
  • the fumigation method contact the Petri dish lid and attach the treated filter paper to the bottom of the Petri dish.
  • Table 2 shows the Melissa (lemon balm) essential oil of Example 1, 3,7-dimethyl-2,6-octadiene and its derivatives, mites, mites, stored food mites, and the like using the fumigation method. The results of a test for the ability to kill small cattle ticks.
  • Melissa (lemon balm) essential oils (2.06, 1.42, 3.25 and 1.97 ⁇ g / cm 2 ), except for octanal, 3,7-dimethyl-2,6-octadiene and derivatives thereof, It showed excellent killing ability against patterned dust mites, preserved food mites and small cattle mites, and showed better killing ability than the control control DEET (27.23, 25.47, 31.47 and 49.72 ⁇ g / cm 2 ).
  • Octanal did not show a potency for storing ticks.
  • Table 3 is a large-legged dust mites, vertical pattern mites, stored food mites and melissa (lemon balm) essential oil of Example 1 and 3,7-dimethyl-2,6-octadiene and its derivatives by using a contact method The results of a test for the ability to kill small cattle ticks.
  • Melissa (lemon balm) essential oils (4.02, 3.34, 7.34 and 0.33 ⁇ g / cm 2 ) and 3,7-dimethyl-2,6-octadiene and derivatives thereof except for octanal are It showed excellent killing ability against pattern dust mites, preserved food ticks and small cattle ticks, and showed 2.35-132.09 times better killing ability than the control group DEET (20.63, 15.13, 17.26 and 43.46 ⁇ g / cm 2 ). Octanal, on the other hand, did not show a potency for storing ticks.
  • Table 4 shows the 3,7-dimethyl-2,6-octadiene and its derivatives (octanal, 2-octenal, 2) contained in the melissa (lemon balm) essential oil of Example 2 using the fumigation method and the contact method. , 4-octadieneal and 3,7-dimethyl-6-octenal) were tested for acaricidal activity against small mite mites.
  • 2-octenal (0.12 ⁇ g / cm 2 ) had the highest killing capacity in contact method, followed by 3,7-dimethyl-6-octenal (0.25 ⁇ g / cm 2 ), 3,7-dimethyl-2, 6-octadiene (0.33 ⁇ g / cm 2 ), 2,4-octadiene (0.53 ⁇ g / cm 2 ), and octane (1.97 ⁇ g / cm 2 ) were shown to have a potency.
  • Melissa (lemon balm) essential oil prepared by the method of Example 1 was tested for acaricide activity by the contact method of Example 3, and then the change of large-legged dust mites, vertical dust mites and stored food mites optical microscope ( ⁇ 500) Observed through.
  • Target tick Color change Melissa essential oil Large leg mites Brown Vertical Pattern Dust Mite Stored food mite 3,7-dimethyl-2,6-octadienol Large leg mites Brown Vertical Pattern Dust Mite Stored food mite Octanal Large leg mites none Vertical Pattern Dust Mite Stored food mite 2-octenal Large leg mites Light brown Vertical Pattern Dust Mite Stored food mite 2,4-octadiene Large leg mites Brown Vertical Pattern Dust Mite Stored food mite 3,7-dimethyl-6-octanal Large leg mites none Vertical Pattern Dust Mite Stored food mite DEET Large leg mites none Vertical Pattern Dust Mite Stored food mite
  • Table 5 shows the results of observing the color change of the large-legged dust mites, vertical patterned mites and stored food mites after treatment with melissa (lemon balm) essential oil.
  • Control, DEET did not discolor the mite (Fig. 1), but Melissa essential oil was changed to orange by inducing the color change of large leg mites, vertical dust mites and stored food mites (Fig. 1).
  • 3,7-dimethyl-2,6-octadieneal, 2-octenal and 2,4-octadieneal induced color changes to mites brown or light brown.
  • the Melissa essential oil, 3,7-dimethyl-2,6-octadieneal, 2-octenal and 2,4-octadieneal can be used as tick markers.
  • Example 5 Example 6 Example 7 Example 8 Example 9 Example 10 Example 11 Example 12 Example 13 Example 14 Example 15 Example 16 Example 17 Example 18
  • the acaricide activity test was performed on house dust mite and small cattle tick, and house dust mite was bred under the condition that the exposure of any acaricide in the room was 25 ⁇ 2 °C, relative humidity 75 ⁇ 10%.
  • Small cattle mites were collected from the Cheonju-si, Jeollabuk-do.
  • the initial dose was set to 104 ⁇ g / cm2 based on the preliminary experiment.
  • the initial dose of the sample was diluted and treated with 50 ⁇ l in filter paper, followed by drying at room temperature (25 ° C.) for 20 minutes.
  • the negative control was treated with only 10 ⁇ l of acetone.
  • the fumigation method is attached to the treated filter paper on the petri dish lid, and the direct contact method is attached to the treated filter paper on the petri dish bottom, and 30 dust mites and 30 small mite ticks are inoculated each to incubate temperature 25 ⁇ 2. °C, relative humidity 75 ⁇ 10%, left for 24 hours in an incubator in the dark conditions and then confirmed whether the tick was alive through the optical microscope.
  • Example 17 (0.21, 0.19, 0.68 ⁇ g / Cm 2) showed the highest acaricide activity, Example 11 (0.46, 0.44, 0.79 ⁇ g / cm 2), Example 13 (0.65, 0.68, 0.88 ⁇ g / cm 2), Example 12 (0.66, 0.59, 0.95 ⁇ g) / Cm 2), Example 5 (0.95, 0.87, 1.28 ⁇ g / cm 2), Example 6 (0.99, 0.93, 1.38 ⁇ g / cm 2), Example 7 (1.17, 1.15, 3.67 ⁇ g / cm 2), Example 8 ( The excellent acaricide activity was shown in the order of 1.32, 1.27 ⁇ g / cm 2), rupture extract (7.05, 6.99, 19.70 ⁇ g / cm 2) and Example 14 (7.53, 6.69 ⁇
  • Example 18 (0.12, 0.19, 0.46 ⁇ g / cm2 ) Showed the highest acaricide activity, Example 11 (0.25, 0.24, 0.56 ⁇ g / cm 2), Example 12 (0.32, 0.32, 0.62 ⁇ g / cm 2), Example 13 (0.35, 0.36, 0.74 ⁇ g / cm 2) ), Example 5 (0.51, 0.47, 0.94 ⁇ g / cm 2), Example 6 (0.53, 0.50, 1.02 ⁇ g / cm 2), Example 7 (0.63, 0.61, 1.84 ⁇ g / cm 2), Example 8 (0.69, 0.71 ⁇ g / cm 2), Example 14 (3.79, 3.45 ⁇ g / cm 2), and rupture extracts (5.57, 5.00, 15.48 ⁇ g / cm 2) showed excellent acaricide activity.
  • FIG. 2 shows an optical microscope picture of a tick fertilized with DEET, which is a conventional chemical control agent
  • FIG. 3 shows an optical microscope picture of a tick fertilized with a compound of Example 11.
  • the specific component of fractional purification of the epoch extract of the present invention is an active ingredient of a natural acaricide, and has an excellent acaricide effect against ticks, and among the active ingredients of the natural acaricide could be used as a visually identifiable indicator for ticks.
  • the compound derived from the melissa essential oil or ruptured extract according to the present invention is an acaricide of natural ingredients, not a conventional chemical control agent, and has excellent flesh against a mite such as a large leg mite, a vertical pattern mite, a stored food mite, and a small grain mite.
  • a mite such as a large leg mite, a vertical pattern mite, a stored food mite, and a small grain mite.
  • a mite such as a large leg mite, a vertical pattern mite, a stored food mite, and a small grain mite.
  • a mite such as a large leg mite, a vertical pattern mite, a stored food mite, and a small grain mite.
  • a mite such as a large leg mite, a vertical pattern mite, a stored food mite, and a small grain mite.
  • it has a labeling activity that can be easily identified by the naked eye by inducing discoloration of

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Abstract

La présente invention concerne un acaricide naturel ayant une activité acaricide contre les acariens, ainsi qu'un marqueur d'identification. Plus spécifiquement, la présente invention concerne un acaricide naturel, un marqueur d'identification ayant pour effet de permettre l'identification d'acariens et leur procédé de fabrication. Ledit acaricide naturel présente une remarquable activité acaricide contre les acariens, tels que les acariens de poussière de maison et Haemaphysalis longicornis, et utilise des substances résultant du fractionnement et de la purification d'huile essentielle de Melissa officinalis (mélisse) ou d'extraits de racine de Morindae officinalis, qui sont des ingrédients naturels et non pas de synthèse.
PCT/KR2015/010828 2014-10-16 2015-10-14 Acaricide naturel contre les acariens, marqueur d'identification pour acariens et leur procédé de fabrication WO2016060462A1 (fr)

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KR1020140140038A KR101661961B1 (ko) 2014-10-16 2014-10-16 진드기용 천연 살비제, 진드기용 식별표시자 및 이의 제조방법
KR10-2014-0140038 2014-10-16
KR1020150134926A KR101787831B1 (ko) 2015-09-23 2015-09-23 진드기용 천연 살비제 및 진드기용 식별 표지자
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KR100777258B1 (ko) * 2006-12-21 2007-11-28 고려바이오주식회사 식물추출물을 이용한 살비제 및 제조방법
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KR20120100970A (ko) * 2009-10-05 2012-09-12 마론 바이오 이노베이션스, 인코포레이티드 생화학 농약 제품으로서의 안트라퀴논 함유 유도체

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KR20010044177A (ko) * 2000-12-23 2001-06-05 안용준 식물에서 추출한 살비성 조성물
KR100624103B1 (ko) * 2004-09-14 2006-09-15 이회선 살비성을 나타내는 식물정유 및 화합물
KR100821926B1 (ko) * 2005-09-21 2008-04-15 재단법인서울대학교산학협력재단 식물 추출물을 포함하는 진드기 알레르기원 중화제 및 이를포함하는 조성물
KR100777258B1 (ko) * 2006-12-21 2007-11-28 고려바이오주식회사 식물추출물을 이용한 살비제 및 제조방법
KR20120100970A (ko) * 2009-10-05 2012-09-12 마론 바이오 이노베이션스, 인코포레이티드 생화학 농약 제품으로서의 안트라퀴논 함유 유도체

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