WO2016058052A1 - Système et procédé de génération d'images numériques de pathologie - Google Patents

Système et procédé de génération d'images numériques de pathologie Download PDF

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Publication number
WO2016058052A1
WO2016058052A1 PCT/AU2015/050635 AU2015050635W WO2016058052A1 WO 2016058052 A1 WO2016058052 A1 WO 2016058052A1 AU 2015050635 W AU2015050635 W AU 2015050635W WO 2016058052 A1 WO2016058052 A1 WO 2016058052A1
Authority
WO
WIPO (PCT)
Prior art keywords
specimen
stage
eyepiece
controller
digital
Prior art date
Application number
PCT/AU2015/050635
Other languages
English (en)
Inventor
Shane BATTYE
Original Assignee
Pathobin Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2014904121A external-priority patent/AU2014904121A0/en
Application filed by Pathobin Pty Ltd filed Critical Pathobin Pty Ltd
Publication of WO2016058052A1 publication Critical patent/WO2016058052A1/fr

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Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/24—Base structure
    • G02B21/26—Stages; Adjusting means therefor
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/362—Mechanical details, e.g. mountings for the camera or image sensor, housings
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes

Definitions

  • Some basic optical microscopes have an observation tube that includes a photo tube in addition to the eyepiece.
  • a photographic camera can be mounted to the photo tube for capturing the image of the portion of the specimen slide that is within the microscope field of view.
  • Digital microscopes use a digital image sensor, such as CCD and CMOS, to generate a digital image of the portion of the specimen slide that is within the microscope field of view.
  • Some digital microscopes have an electronic powered stage, in which movement of the microscope stage is performed by electric motors. The combination of a digital image sensors and an electronic powered stage provides the capacity to generate large, high-resolution pathology images by capturing a series of digital images and using software to "stitch" these images together.
  • Capturing a pathology image digitally has the advantage that the image can be readily transmitted to a separate location that is geographically spaced from the actual microscope and specimen via a computer network, enabling an operator - such as a pathologist - to review and analyze the image at the separate location. While there are advantages in digital pathology images, there are also significant equipment costs associated with digital microscopes. There is a need to address the above, and/or at least provide a useful alternative.
  • a powered drive system that includes two or more electric actuators that, in use of the system, are each operable to cause the specimen slide to move in one of two orthogonal directions beneath the objective;
  • each carriage being mounted on a respective one of the first support member and being displaceable along the respective first support member by operation of one or more first electric actuators,
  • the system can further include a support structure for supporting the powered drive system above a surface on which the optical microscope rests.
  • the present invention also provides a system for generating a digital pathology image from an optical microscope having a mechanical stage on which a specimen slide is to be mounted, the stage being movable in two orthogonal directions by manually- operated stage controllers, and an eyepiece or photo tube through which to view a specimen on the specimen slide, the system including: a first mount that is connectable to the eyepiece or photo tube, and is configured to support a digital image capture device so as to capture digital images of the specimen as viewed through the eyepiece;
  • the powered drive system includes, for each of the stage controllers, an electric actuator with an output drive member, and a drive train for transferring motion of the drive member to the respective stage controller.
  • each drive train further includes an output pulley that is releasably mountable to the respective stage controller, and each belt extends around the respective input and output pulleys.
  • Each output pulley can have a central aperture to locate over the respective stage controller, and one or more locking screws that pass through a screw hole extending from a peripheral edge of the output pulley and open onto the central aperture.
  • the image capture device can include a processing unit that is configured to generate an output signal that includes information representative of the magnitude of movement of the stage in each of the orthogonal directions to index the microscope stage to position corresponding with that of the next digital image to be captured in the series, whereby the output signal is provides the input to the controller.
  • the present invention provides a method of generating a digital pathology image from an optical microscope having a mechanical stage on which a specimen slide is to be mounted, the stage being movable in two orthogonal directions by manually-operated stage controllers, and an eyepiece or photo tube through which to view a specimen on the specimen slide, the method involving:
  • the method may involve cropping each raw digital image captured by the image capture device to remove redundant information to form each of the overlapping digital images.
  • the method further involves stitching at least some of the overlapping digital images to form the digital pathology image.
  • the method may involve transmitting the series of overlapping digital images to another location that is remote from the microscope.
  • the stitching step may be performed at the other location.
  • the co-ordinating step preferably involves alternately capturing one of the overlapping digital images in the series, and indexing the stage to a position corresponding with the position of subsequent overlapping digital image in the series.
  • the method can further involve:
  • the method can further involve identifying the objective power of the microscope to be used during capture of the series, wherein the step of determining the set of microscope stage indices is also based on the objective power, and the microscope field of view.
  • the method further involves:
  • the step of determining the set of microscope stage indices is also based on the initial position.
  • the digital image capture device includes a processor that is configured to generate an output signal that includes information representative of the magnitude of movement of the stage in each of the orthogonal directions to index the microscope stage to position corresponding with that of the next digital image to be captured in the series, whereby the output signal is provides the input to the controller, and whereby the method further involves instructing the image capture device to commence the co-ordinating step.
  • the communications interface sends one or more output signals for an external controller to control a system for electronically driving the stage;
  • the plurality of images form a series of overlapping digital images of the object.
  • the image capture device can receive inputs that include any one or more of: one or more geometric properties of the object of which the plurality of images are to be captured;
  • Figure 1 is a front view of an optical microscope, and a system for use in generating digital pathology images, the system being in accordance with a first embodiment of the present invention
  • Figure 2 is a first elevation view of region A in Figure 1 ;
  • Figure 3 is a second elevation view of region A in Figure 1 ;
  • Figure 8 is a schematic view of the operation of the system of Figure 1 ;
  • Figure 13 is a front view of an optical microscope, and a system for use in generating digital pathology images, the system being in accordance with a third embodiment of the present invention
  • steps 102 to 108 do not need to be performed in this sequence.
  • steps 104 and 106 may be performed once during an initial setup of the microscope.
  • Steps 102 and 108 can be performed for a number of different samples, whether on the same specimen slide, or on separate specimen slides.
  • Figures 13 and 14 show a system 310 according to a third embodiment of the present invention.
  • the system 310 is capable of generating a digital pathology image from an optical microscope 60 that has an eyepiece 68, and an objective 70 beneath which a specimen slide is to be positioned.
  • the optical microscope 60 has a light path that extends between the eyepiece 68 and the objective 70.
  • a specimen on the specimen slide that is placed beneath the objective 70 is viewable through the eyepiece 68.
  • the system 310 is substantially similar to the system 10 of Figures 1 to 8. In
  • the pincers 380 extend from a platform 382 of the powered drive 314.
  • a drive train coupled to the platform 382 is driven by the stepper motors 334a, 334b, 336.
  • movement of each of the stepper motors 334a, 334b, 336 is transferred through the drive train to cause the platform 382 to move in a respective one of the orthogonal directions.
  • Stepper motors 334a, 334b operate synchronously to move the X-direction carriages 386a, 386b along their respective guide rods 384a, 384b.
  • the first drive has two threaded rods 388a, 388b that are each connected to one of the two X- stepper motors 334a, 334b.
  • Each threaded rod 388a, 388b extends parallel to one of the guide rods 384a, 384b, and passes through an internally threaded hole in the respective X-direction carriage 386a, 386b.

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

La présente invention se rapporte à un système qui permet de générer une image numérique de pathologie à partir d'un élément optique. Le système comprend : un premier support qui peut être relié à un oculaire ou à un phototube, et qui est conçu pour porter un dispositif de capture d'image numérique de façon à capturer des images numériques de l'échantillon vu à travers l'oculaire ou le phototube ; un système d'entraînement alimenté qui inclut au moins deux actionneurs électriques pouvant servir, lors de l'utilisation du système, à provoquer le déplacement de la lame d'échantillon dans une direction parmi deux directions perpendiculaires en dessous de l'objectif ; ainsi qu'un contrôleur qui est en communication avec le système d'entraînement alimenté, ce contrôleur étant prévu pour recevoir une entrée qui représente un ordre de déplacement de la lame d'échantillon, et, en réponse à cette entrée, pour diriger le système d'entraînement alimenté de manière à déplacer ladite lame d'échantillon. La coordination du fonctionnement du dispositif de capture d'image et de la transmission d'entrées au contrôleur permet de capturer une série d'images numériques superposées servant à générer l'image numérique de pathologie de l'échantillon.
PCT/AU2015/050635 2014-10-15 2015-10-15 Système et procédé de génération d'images numériques de pathologie WO2016058052A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2014904121A AU2014904121A0 (en) 2014-10-15 System And Method For Generating Digital Pathology Images
AU2014904121 2014-10-15

Publications (1)

Publication Number Publication Date
WO2016058052A1 true WO2016058052A1 (fr) 2016-04-21

Family

ID=55745885

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AU2015/050635 WO2016058052A1 (fr) 2014-10-15 2015-10-15 Système et procédé de génération d'images numériques de pathologie

Country Status (1)

Country Link
WO (1) WO2016058052A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020089289A3 (fr) * 2018-10-31 2020-06-11 Spotlab, S.L. Système d'activation d'acquisition numérique d'un échantillon au moyen d'un instrument optique
CN113572912A (zh) * 2021-07-03 2021-10-29 冯浩 一种数字病理切片的远程传输方法
CN113640231A (zh) * 2021-09-15 2021-11-12 重庆医科大学 高速自动显微成像装置

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6711283B1 (en) * 2000-05-03 2004-03-23 Aperio Technologies, Inc. Fully automatic rapid microscope slide scanner
WO2006023675A2 (fr) * 2004-08-18 2006-03-02 Tripath Imaging, Inc. Systeme de microscopie dote de modes interactif et automatique pour former une image en mosaique agrandie et procede associe
US7088918B1 (en) * 2005-01-21 2006-08-08 Leica Microsystems (Schweiz) Ag Mounting device for a camera with automatic exposure mode to an optical system
CN202041714U (zh) * 2011-04-21 2011-11-16 莲都区梅山中学 相机显微镜

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6711283B1 (en) * 2000-05-03 2004-03-23 Aperio Technologies, Inc. Fully automatic rapid microscope slide scanner
WO2006023675A2 (fr) * 2004-08-18 2006-03-02 Tripath Imaging, Inc. Systeme de microscopie dote de modes interactif et automatique pour former une image en mosaique agrandie et procede associe
US7088918B1 (en) * 2005-01-21 2006-08-08 Leica Microsystems (Schweiz) Ag Mounting device for a camera with automatic exposure mode to an optical system
CN202041714U (zh) * 2011-04-21 2011-11-16 莲都区梅山中学 相机显微镜

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020089289A3 (fr) * 2018-10-31 2020-06-11 Spotlab, S.L. Système d'activation d'acquisition numérique d'un échantillon au moyen d'un instrument optique
CN113572912A (zh) * 2021-07-03 2021-10-29 冯浩 一种数字病理切片的远程传输方法
CN113640231A (zh) * 2021-09-15 2021-11-12 重庆医科大学 高速自动显微成像装置

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