WO2016037483A1 - 同轴线双高压缸均质一体化装置 - Google Patents

同轴线双高压缸均质一体化装置 Download PDF

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WO2016037483A1
WO2016037483A1 PCT/CN2015/075448 CN2015075448W WO2016037483A1 WO 2016037483 A1 WO2016037483 A1 WO 2016037483A1 CN 2015075448 W CN2015075448 W CN 2015075448W WO 2016037483 A1 WO2016037483 A1 WO 2016037483A1
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Prior art keywords
cylinder
pressure
pressure cylinder
high pressure
valve
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PCT/CN2015/075448
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English (en)
French (fr)
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余行文
余茜
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广州聚能生物科技有限公司
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Priority to US15/318,674 priority Critical patent/US10584308B2/en
Priority to DK15839508.7T priority patent/DK3162884T3/en
Priority to JP2017503167A priority patent/JP6431177B2/ja
Priority to EP15839508.7A priority patent/EP3162884B1/en
Priority to ES15839508.7T priority patent/ES2690143T3/es
Priority to CA2956292A priority patent/CA2956292C/en
Publication of WO2016037483A1 publication Critical patent/WO2016037483A1/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C19/00—Other disintegrating devices or methods
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C19/00—Other disintegrating devices or methods
    • B02C19/18—Use of auxiliary physical effects, e.g. ultrasonics, irradiation, for disintegrating
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/33—Disintegrators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/14—Pressurized fluid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/08—Homogenizing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B31/00—Free-piston pumps specially adapted for elastic fluids; Systems incorporating such pumps
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B9/00—Piston machines or pumps characterised by the driving or driven means to or from their working members
    • F04B9/08—Piston machines or pumps characterised by the driving or driven means to or from their working members the means being fluid
    • F04B9/10—Piston machines or pumps characterised by the driving or driven means to or from their working members the means being fluid the fluid being liquid
    • F04B9/109—Piston machines or pumps characterised by the driving or driven means to or from their working members the means being fluid the fluid being liquid having plural pumping chambers
    • F04B9/111—Piston machines or pumps characterised by the driving or driven means to or from their working members the means being fluid the fluid being liquid having plural pumping chambers with two mechanically connected pumping members
    • F04B9/113—Piston machines or pumps characterised by the driving or driven means to or from their working members the means being fluid the fluid being liquid having plural pumping chambers with two mechanically connected pumping members reciprocating movement of the pumping members being obtained by a double-acting liquid motor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C19/00—Other disintegrating devices or methods
    • B02C19/18—Use of auxiliary physical effects, e.g. ultrasonics, irradiation, for disintegrating
    • B02C2019/183—Crushing by discharge of high electrical energy

Definitions

  • the invention relates to a pressure homogenizing device for a cell crusher, in particular to a coaxial double-high pressure cylinder homogenizing integrated device.
  • Cell disruption technology refers to the technique of destroying cell membranes and cell walls by external force, and releasing the contents of cells including the target product components, and is the basis for separating and purifying non-secretory biochemical substances (products) synthesized in cells. Combined with significant advances in recombinant DNA technology and tissue culture technology, proteins previously thought to be difficult to obtain are now available for mass production. A variety of cell disruption methods have been developed to accommodate different uses and different types of cell wall disruption. The crushing method can be classified into two major categories: mechanical methods and non-mechanical methods. There are many kinds of equipment used in the mechanical method, and the commonly used equipment is an ultra-high pressure cell crushing device.
  • the commonly used equipment is broken and finely dispersed in biological cells, medicines, foods, milk, cosmetics, chemical materials, nano materials, and the like. It has a wide range of applications in particle nanocrystallization and emulsion processing.
  • the disadvantages of the commonly used ultra-high pressure cell crushing device are: (1) using a single-shaft main oil cylinder, which has high energy consumption; (2) the liquid outlet of the pressure homogenizing device is opened at the upper portion, and the liquid discharge is not smooth and easy. (3) The pressure gauge of the pressure homogenizing device is directly connected with the high pressure chamber, so that the high pressure meter frequently pulsates and is easily damaged; (4) the high pressure energy release is easy to generate temperature rise; (5) the high pressure homogenizing valve is easy abrasion. It is therefore necessary to invent a new pressurized homogenizing device to improve the aforementioned drawbacks.
  • the object of the present invention is to provide a coaxial double-high pressure cylinder homogenizing integrated device, which has a compact structure, saves parts, is advantageous for miniaturization, and is energy-saving.
  • the present invention provides a coaxial double-high pressure cylinder homogenizing integrated device, which comprises a long oil cylinder, a main connecting sleeve, a high-pressure cylinder homogeneous main body, a connecting sleeve and a short oil cylinder, and the main connecting sleeve, There are two high-pressure cylinder homogeneous main bodies, attached connecting sleeves and short oil cylinders respectively.
  • the two main connecting sleeves, two high-pressure cylinder homogeneous main bodies, two connecting connecting sleeves and two short oil cylinders are respectively symmetrically arranged at Both ends of the long cylinder are installed coaxially with the long cylinder, and the homogeneous bodies of the high-pressure cylinders are integrally connected with the long cylinder through a main connecting sleeve, and the high-pressure cylinders are homogeneous bodies.
  • the long oil cylinder is provided with a piston rod which can be alternately extended from both ends thereof, and two ends of the piston rod are respectively connected to one of the high pressure cylinders a pressure plunger rod in a high pressure chamber of the main body, and a homogenizing valve in the inner cavity of each of the high pressure cylinder homogeneous bodies is respectively connected to a top rod of the short cylinder
  • the structure is compact and small, Conducive to miniaturization design, and the long cylinder has piston rods that can be alternately extended from both ends, so that the high-pressure cylinders of both sides can alternately work to crush the cells, compared with the prior art.
  • the high-pressure cylinder homogenizing integrated device of the long oil cylinder, the coaxial double-high pressure cylinder homogenizing integrated device has obvious power saving, at least energy saving 30%-40%, and the energy consumption is greatly reduced.
  • an upper portion of the high pressure chamber end of each of the high pressure cylinders is provided with a feed port communicating with the high pressure chamber, and a left side of each of the high pressure cylinders is connected to the high pressure chamber.
  • a pressure gauge connection port, a right side of each of the high-pressure cylinder homogeneous bodies is further provided with a liquid outlet connected to the sample chamber of the high-pressure cylinder homogeneous body, and the liquid outlet is connected to the bottom of the sample chamber and vertically And connect the stainless steel cooling coil.
  • the liquid outlet is opened on the top of the homogeneous body of the high-pressure cylinder, the discharge resistance is smaller, the liquid discharge is smoother, and it is not easy to remain in the homogeneous body of the high-pressure cylinder.
  • each of the feed ports is connected with an integrated feeding device
  • each of the integrated feeding devices includes a second one-way valve and a storage cup, and the second one-way valve
  • An inlet of the valve seat is directly connected to a bottom of the storage cup
  • an outlet of a valve body of each of the second one-way valves is connected to a corresponding inlet
  • a valve body of the second one-way valve is
  • the storage cup has a unitary structure. The structure can facilitate the injection and exhaust, and can further make the structure of the coaxial double-high pressure cylinder homogenization integrated device more compact, thereby facilitating the miniaturization design, and also reducing the loss of materials in the crushing, and the operation is convenient. .
  • each of the pressure gauge connection ports is provided with a first one-way valve, and a part of the valve body of each of the first one-way valves is located in a corresponding high-pressure cylinder homogeneous body and is connected with a high-pressure cylinder Homogenization
  • the main body constitutes a monolithic structure.
  • the structure can make the structure of the coaxial double-high pressure cylinder homogenizing integrated device more compact, thereby facilitating the miniaturization design, and also preventing the high pressure meter from pulsing and avoiding damage of the high pressure gauge.
  • the axis line of each of the high pressure cylinder homogeneous bodies intersects perpendicularly with the axis lines of the respective first check valve and the second check valve.
  • a flat contact seal is formed between the valve core and the valve seat of each of the first check valves, and a planar contact seal is also formed between the valve core and the valve seat of each of the second check valves.
  • the upper part of the main connecting sleeve is provided with a main observation adjusting window, and the lower part is provided with a water circulation window, the upper part of the connecting sleeve is provided with an observation adjusting window, and the lower part is provided with a water recirculation window.
  • This structure facilitates observation and adjustment as well as cooling water circulation.
  • Fig. 1 is a front elevational view showing the coaxial double-high pressure cylinder homogenizing device of the present invention.
  • FIG. 2 is a left side view of the coaxial double-high pressure cylinder homogenizing device of the present invention.
  • FIG 3 is a cross-sectional view of the high pressure cylinder homogeneous body and the first check valve.
  • Figure 4 is a cross-sectional view of the integrated feeder.
  • the coaxial double-high pressure cylinder homogenizing integrated device of the present invention comprises a long oil cylinder 1, two main connecting sleeves, two high-pressure cylinder homogeneous main bodies 3, two connecting sleeves 4 and Two short cylinders 5. Place The two ends of the long cylinder 1 are respectively connected to one end of a high pressure cylinder homogeneous body 3 through a main connecting sleeve 2, and the other end of each high pressure cylinder homogeneous body 3 is connected to a short cylinder 5 through a connecting sleeve 4.
  • the long oil cylinder 1, the main connecting sleeve 2, the high pressure cylinder homogeneous main body 3, the connecting sleeve 4 and the short oil cylinder 5 are all screwed, and the long oil cylinder 1, the two main connecting sleeves 2, two A high-pressure cylinder homogeneous body 3, two connecting sleeves 4 and two short cylinders 5 are coaxially mounted.
  • the long cylinder 1 is provided with a piston rod which can be alternately extended from both ends thereof, and the short cylinder 5 is provided with a plunger 51 which can protrude from one end thereof.
  • a main viewing adjustment window 21 is defined in the upper portion of each of the main connecting sleeves 2, and a water circulation window 22 is defined in the lower portion.
  • the upper portion of each of the connecting sleeves 4 is provided with an observation adjusting window 41, and the lower portion is provided with a water replenishing circulation window 42.
  • the main observation adjustment window 21 and the observation adjustment window 41 can be conveniently observed and adjusted, and the water circulation window 22 and the water distribution circulation window 42 can facilitate the cooling water circulation.
  • Each of the high-pressure chambers of the high-pressure cylinder homogeneous body 3 has a reciprocable pressure plunger rod 31, and two ends of the piston rod of the long cylinder 1 are respectively connected to a high-pressure chamber of the high-pressure cylinder homogeneous body 3 a pressurized plunger rod 31; a homogenizing valve in the inner cavity of each of the high-pressure cylinder homogeneous bodies 3 communicating with the high-pressure chamber, and a homogenizing valve in the inner cavity of each of the high-pressure cylinders
  • the ejector pins 51 of the short oil cylinders 5 are respectively connected, that is, the two-way oil cylinders are used on both sides of the high-pressure cylinder homogeneous body 3, thereby greatly simplifying the structure of the conventional whole machine, reducing the connecting parts and the one-way valves, and reducing the failure rate.
  • the long cylinder 1 is a main cylinder that presses the plunger rod 31, and the short cylinder 5 is an auxiliary cylinder that controls the crushing pressure.
  • a pressure gauge connection port communicating with the high pressure chamber is disposed on a left side of each of the high pressure cylinder homogeneous bodies 3, and a first check valve 6 is disposed in the pressure gauge connection port.
  • An upper portion of the high pressure chamber end of each of the high pressure cylinders is provided with a feed port communicating with the high pressure chamber, and each of the feed ports is connected with an integrated feeding device 7 so that the integrated feeding device 7 is connected. At the upper end of the corresponding high pressure chamber, it is easy to inject and vent.
  • each of the high-pressure cylinder homogeneous bodies 3 is further provided with a liquid outlet 9 which communicates with the bottom of the sample chamber and is vertically downward and is connected to the stainless steel cooling coil 8 (Figs. 1 and 2). Only a part of the stainless steel cooling coil 8 is shown, so that the resistance is small, the liquid discharge is smooth, and it is not easy to remain in the homogeneous body of the high pressure cylinder.
  • each of the first check valves 6 includes a first valve body 61, a first valve body 62 and a first valve seat 63.
  • the first valve body 62 and the first valve seat 63 are located in the passage of the first valve body 61
  • the first valve core 62 and the first valve seat 63 are in a planar contact seal, so that the working stability is good and the service life is long.
  • each of the first valve bodies 61 is located in the corresponding high-pressure cylinder homogeneous body 3 and forms an integral structure with the high-pressure cylinder homogeneous body 3, and the other part is located outside the high-pressure cylinder homogeneous body 3, thereby improving the same
  • the stability of the axis double high pressure cylinder homogenizing integrated device prevents the high pressure meter from pulsing, avoids damage of the high pressure meter, and has a compact structure, which is advantageous for miniaturization design.
  • Each of the integrated feeding devices 7 includes a second one-way valve 71 and a hopper cup 72, and a shaft line of each of the high-pressure cylinder homogeneous bodies 3 and the first one-way valve and the second one-way valve
  • the axial lines of the 71 intersect perpendicularly, which is more advantageous for the pressure gauge to accurately detect the pressure in the pressurized homogenizing integrated device, and is advantageous for miniaturization design.
  • Each of the second check valves 71 includes a second valve body 71a, a second valve body 71b, and a second valve seat 71c.
  • the second valve body 71b and the second valve seat 71c are located in the passage of the second valve body 71a, and the second valve body 71b and the second valve seat 71c are in a planar contact seal, thereby working stability. Good, long life.
  • the second valve body 71a and the hopper cup 72 are of a unitary structure.
  • An inlet 711 is defined in the second valve seat 71c, and the inlet 711 is directly connected to the bottom of the storage cup 72, and the outlet of each of the second valve bodies 71a is connected with a corresponding inlet, thereby eliminating the need for
  • the long connecting pipe can greatly reduce the loss of the sample material in the crushing; at the same time, the second check valve 71 can directly insert the exhaust gas from the storage cup 72 by using the steel needle, and the operation is very convenient and easy.
  • the hopper cup 72 is provided with a cup cover 73.
  • the cup cover 73 is sleevedly connected to the hopper cup 72, and may also be screwed to facilitate the addition of the sample and the internal exhaust of the second one-way valve 71.
  • the cup lid 73 is removed.
  • the cup lid 73 is provided with a quick pipe joint 74 for quick cleaning to facilitate the cleaning operation of the storage cup 72.

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Abstract

公开了一种同轴线双高压缸均质一体化装置，包括长油缸、两个主连接套、两个高压缸均质主体、两个附连接套和两个短油缸，两个主连接套、两个高压缸均质主体、两个附连接套和两个短油缸均分别对称设置在所述长油缸的两端并且均与长油缸同轴心线安装，各高压缸均质主体通过一主连接套与所述长油缸连接成整体，各高压缸均质主体通过一附连接套与相应的短油缸连接成整体，长油缸内设有可从其两端交替伸出的活塞杆，活塞杆的两端分别连接一高压缸均质主体的高压腔内的加压柱塞杆，各高压缸均质主体的内腔内的均质阀均分别连接一短油缸的顶杆。

Description

同轴线双高压缸均质一体化装置 技术领域
本发明涉及一种细胞破碎仪用加压均质装置，尤其涉及一种同轴线双高压缸均质一体化装置。
背景技术
细胞破碎技术是指利用外力破坏细胞膜和细胞壁，使细胞内容物包括目的产物成分释放出来的技术，是分离纯化细胞内合成的非分泌型生化物质(产品)的基础。结合重组DNA技术和组织培养技术上的重大进展，以前认为很难获得的蛋白质现在可以大规模生产。目前已发展了多种细胞破碎方法，以便适应不同用途和不同类型的细胞壁破碎。破碎方法可规纳为机械法和非机械法两大类。而机械法所用到的设备也有很多种，而常用的设备是超高压细胞破碎装置，该常用的设备在生物细胞、药物、食品、牛奶、化妆品、化工材料、纳米材料等的破碎、微细分散、颗粒纳米化、乳化加工中具有广泛的用途。该常用的超高压细胞破碎装置存在的缺点是：(1)采用单轴式主油缸，能耗高；(2)其加压均质装置的出液口开在上部，出液不顺畅，容易有残留；(3)其加压均质装置的压力表与高压腔直接连接，使得高压表频繁脉动，从而容易损坏；(4)高压能量释放容易产生温升；(5)高压均质阀容易磨损。因此有必要发明一种新的加压均质装置，以改善前述缺陷。
发明内容
本发明的目的是提供一种同轴线双高压缸均质一体化装置，结构紧凑，节省零件，利于小型化，且节能。
为实现上述目的，本发明提供一种同轴线双高压缸均质一体化装置，包括长油缸、主连接套、高压缸均质主体、附连接套和短油缸，所述的主连接套、 高压缸均质主体、附连接套和短油缸均分别有两个，所述的两个主连接套、两个高压缸均质主体、两个附连接套和两个短油缸均分别对称设置在所述长油缸的两端并且均与长油缸同轴心线安装，各所述高压缸均质主体通过一所述主连接套与所述长油缸连接成整体，各所述高压缸均质主体通过一所述附连接套与相应的短油缸连接成整体，所述长油缸内设有可从其两端交替伸出的活塞杆，所述活塞杆的两端分别连接一所述高压缸均质主体的高压腔内的加压柱塞杆，各所述高压缸均质主体的内腔内的均质阀均分别连接一所述短油缸的顶杆
由于两个主连接套、两个高压缸均质主体、两个附连接套和两个短油缸对称设置在长油缸的两侧并且与长油缸同轴心线安装，从而结构紧凑，体积小，有利于小型化设计，而且长油缸有可从其两端交替伸出的活塞杆，从而可以交替的使两边的高压缸均质主体工作对细胞进行破碎，相比现有技术中的使用单轴式长油缸的高压缸均质一体化装置，本同轴线双高压缸均质一体化装置节电明显，至少可节能30％-40％，使能耗大大降低。
作为本发明的进一步改进，各所述高压缸均质主体的高压腔尾端上部设有与高压腔连通的进料口，各所述高压缸均质主体的左侧设有与高压腔连通的压力表连接口，各所述高压缸均质主体的右侧还设有与高压缸均质主体的出样腔连通的出液口，所述出液口与出样腔底部相连通并且垂直向下且连接不锈钢冷却盘管。此结构相比现有技术中出液口开在高压缸均质主体顶部的方式，出液阻力更小，出液更加顺畅，不易残留在高压缸均质主体内。
作为本发明的更进一步改进，各所述进料口连接有一个一体化进料装置，各所述一体化进料装置包括第二单向阀和贮料杯，所述第二单向阀的阀座的进口与所述贮料杯的底部直接连接，各所述第二单向阀的阀体的出口与相应的进料口连接，并且所述第二单向阀的阀体与所述贮料杯为整体式结构。此结构可便于进样与排气，还可以使得同轴线双高压缸均质一体化装置的结构更加紧凑，从而有利于小型化设计，且还可以减少物料在破碎中的损耗，而且操作方便。
作为本发明的更进一步改进，各所述压力表连接口内设有第一单向阀，各所述第一单向阀的阀体的一部分位于相应的高压缸均质主体内并与高压缸均质 主体构成整体式结构。此结构可使同轴线双高压缸均质一体化装置的结构更加紧凑，从而有利于小型化设计，而且还可防止高压表脉动，避免高压表损坏。
作为本发明的更进一步改进，各所述高压缸均质主体的轴心线与相应的第一单向阀和第二单向阀的轴心线垂直相交。此结构有利于压力表准确检测高压缸均质主体内的压力，且有利于小型化设计，而且还更有利于压力表准确检测加压均质一体化装置内的压力。
作为本发明的更进一步改进，各所述第一单向阀的阀芯和阀座之间为平面接触密封，各所述第二单向阀的阀芯和阀座之间也为平面接触密封。此结构加工方便，而且第一单向阀和第二单向阀的工作稳定性好，使用寿命长。
作为本发明的另一种改进，所述主连接套的上部开设有主观察调整窗口，下部开设有水循环窗口，所述附连接套的上部开设有附观察调整窗口，下部开设有附水循环窗口。此结构可以方便进行观察和调整以及冷却水循环。
通过以下的描述并结合附图，本发明将变得更加清晰，这些附图用于解释本发明的实施例。
附图说明
图1为本发明的同轴线双高压缸均质一体化装置的主视图。
图2为本发明的同轴线双高压缸均质一体化装置的左视图。
图3为高压缸均质主体和第一单向阀的剖视图。
图4为一体化进料装置的剖视图。
具体实施方式
现在参考附图描述本发明的实施例，附图中类似的元件标号代表类似的元件。
请参考图1-图4，本发明的同轴线双高压缸均质一体化装置包括长油缸1、两个主连接套2、两个高压缸均质主体3、两个附连接套4和两个短油缸5。所 述长油缸1的两端分别通过一个主连接套2连接至一个高压缸均质主体3的一端，各高压缸均质主体3的另一端通过一附连接套4连接至一短油缸5。所述的长油缸1、主连接套2、高压缸均质主体3、附连接套4和短油缸5之间均通过螺纹连接，且所述的长油缸1、两个主连接套2、两个高压缸均质主体3、两个附连接套4和两个短油缸5同轴心线安装。
进一步说明的是，所述长油缸1内设有可从其两端交替伸出的活塞杆，所述短油缸5内设有可从其一端伸出的顶杆51。
各所述主连接套2的上部开设有主观察调整窗口21，下部开设有水循环窗口22，各所述附连接套4的上部开设有附观察调整窗口41，下部开设有附水循环窗口42。所述的主观察调整窗口21和附观察调整窗口41可以方便进行观察和调整，所述的水循环窗口22和附水循环窗口42可以方便冷却水循环。
各所述高压缸均质主体3的高压腔内有可往复移动的加压柱塞杆31，所述长油缸1的活塞杆的两端分别连接一所述高压缸均质主体3的高压腔内的加压柱塞杆31；各所述高压缸均质主体3的与高压腔连通的内腔内还有均质阀，各所述高压缸均质主体3的内腔内的均质阀均分别连接一所述短油缸5的顶杆51，即高压缸均质主体3的两侧采用双向油缸，从而可以大大简化传统整机结构，减少连接件和单向阀，降低了故障率，便于检验及配件维修更换。所述长油缸1是加压柱塞杆31运动的主油缸，所述短油缸5是控制破碎压力的附油缸。各所述高压缸均质主体3的左侧设有与高压腔连通的压力表连接口，所述压力表连接口内设有第一单向阀6。各所述高压缸均质主体3的高压腔尾端上部设有与高压腔连通的进料口，各所述进料口连接有一体化进料装置7，从而使得一体化进料装置7连接在相应的高压腔的尾端上部，从而便于进样与排气。各所述高压缸均质主体3的右侧还设有出液口9，所述出液口9与出样腔底部相连通并且垂直向下且连接不锈钢冷却盘管8(图1和图2中只示出不锈钢冷却盘管8的一部分)，从而阻力小，出液顺畅，不易残留在高压缸均质主体内。
更进一步说明的是，各所述第一单向阀6包括第一阀体61、第一阀芯62和第一阀座63。所述第一阀芯62和所述第一阀座63位于所述第一阀体61的通道 内，且所述第一阀芯62和第一阀座63之间为平面接触密封，从而工作稳定性好，使用寿命长。各所述第一阀体61的一部分位于相应的高压缸均质主体3内并与所述高压缸均质主体3构成整体式结构，另一部分位于高压缸均质主体3外，从而可以提高同轴线双高压缸均质一体化装置的稳定性，防止高压表脉动，避免高压表损坏，而且结构紧凑，有利于小型化设计。
各所述一体化进料装置7包括第二单向阀71和贮料杯72，各所述高压缸均质主体3的轴心线与所述的第一单向阀、第二单向阀71的轴心线垂直相交，从而更有利于压力表准确检测加压均质一体化装置内的压力，且有利于小型化设计。各所述第二单向阀71包括第二阀体71a、第二阀芯71b和第二阀座71c。所述第二阀芯71b和第二阀座71c位于所述第二阀体71a的通道内，且所述第二阀芯71b和第二阀座71c之间为平面接触密封，从而工作稳定性好，使用寿命长。所述第二阀体71a与所述贮料杯72为整体式结构。所述第二阀座71c内设有进口711，所述进口711与所述贮料杯72的底部直接连接，各所述第二阀体71a的出口与相应的进料口连接，从而不需要长的连接管路，可大大减少样品物料在破碎中的损耗；同时，第二单向阀71排气时可以直接用钢针从贮料杯72中插入排气，操作非常方便容易。所述贮料杯72上设有杯盖73，所述杯盖73与贮料杯72套接连接，也可以是螺纹连接，以方便添加样品及所述第二单向阀71内部排气时将所述杯盖73取下。所述杯盖73上设有用于快速清洗的快速管接头74，以方便对贮料杯72清洗操作。
以上结合最佳实施例对本发明进行了描述，但本发明并不局限于以上揭示的实施例，而应当涵盖各种根据本发明的本质进行的修改、等效组合。

Claims (7)

  1. 一种同轴线双高压缸均质一体化装置，包括长油缸、主连接套、高压缸均质主体、附连接套和短油缸，其特征在于：所述的主连接套、高压缸均质主体、附连接套和短油缸均分别有两个，所述的两个主连接套、两个高压缸均质主体、两个附连接套和两个短油缸均分别对称设置在所述长油缸的两端并且均与长油缸同轴心线安装，各所述高压缸均质主体通过一所述主连接套与所述长油缸连接成整体，各所述高压缸均质主体通过一所述附连接套与相应的短油缸连接成整体，所述长油缸内设有可从其两端交替伸出的活塞杆，所述活塞杆的两端分别连接一所述高压缸均质主体的高压腔内的加压柱塞杆，各所述高压缸均质主体的内腔内的均质阀均分别连接一所述短油缸的顶杆。
  2. 如权利要求1所述的同轴线双高压缸均质一体化装置，其特征在于：各所述高压缸均质主体的高压腔尾端上部设有与高压腔连通的进料口，各所述高压缸均质主体的左侧设有与高压腔连通的压力表连接口，各所述高压缸均质主体的右侧还设有与高压缸均质主体的出样腔连通的出液口，所述出液口与出样腔底部相连通并且垂直向下且连接不锈钢冷却盘管。
  3. 如权利要求2所述的同轴线双高压缸均质一体化装置，其特征在于：各所述进料口连接有一个一体化进料装置，各所述一体化进料装置包括第二单向阀和贮料杯，所述第二单向阀的阀座的进口与所述贮料杯的底部直接连接，各所述第二单向阀的阀体的出口与相应的进料口连接，并且所述第二单向阀的阀体与所述贮料杯为整体式结构。
  4. 如权利要求3所述的同轴线双高压缸均质一体化装置，其特征在于：各所述压力表连接口内设有第一单向阀，各所述第一单向阀的阀体的一部分位于相应的高压缸均质主体内并与高压缸均质主体构成整体式结构。
  5. 如权利要求4所述的同轴线双高压缸均质一体化装置，其特征在于：各所述高压缸均质主体的轴心线与相应的第一单向阀和第二单向阀的轴心线垂直相交。
  6. 如权利要求5所述的同轴线双高压缸均质一体化装置，其特征在于：各所述第一单向阀的阀芯和阀座之间为平面接触密封，各所述第二单向阀的阀芯 和阀座之间也为平面接触密封。
  7. 如权利要求1所述的同轴线双高压缸均质一体化装置，其特征在于：所述主连接套的上部开设有主观察调整窗口，下部开设有水循环窗口，所述附连接套的上部开设有附观察调整窗口，下部开设有附水循环窗口。
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CA2956292A1 (en) 2016-03-17
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ES2690143T3 (es) 2018-11-19
US10584308B2 (en) 2020-03-10
DK3162884T3 (en) 2018-10-29
CN104195038B (zh) 2016-06-15
US20170130185A1 (en) 2017-05-11
JP6431177B2 (ja) 2018-11-28
EP3162884A1 (en) 2017-05-03
CA2956292C (en) 2021-08-24
CN104195038A (zh) 2014-12-10
JP2017527270A (ja) 2017-09-21

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