WO2016028177A1 - Liquid biostimulator increasing resistance of agricultural plants to stress conditions - Google Patents
Liquid biostimulator increasing resistance of agricultural plants to stress conditions Download PDFInfo
- Publication number
- WO2016028177A1 WO2016028177A1 PCT/PL2015/050031 PL2015050031W WO2016028177A1 WO 2016028177 A1 WO2016028177 A1 WO 2016028177A1 PL 2015050031 W PL2015050031 W PL 2015050031W WO 2016028177 A1 WO2016028177 A1 WO 2016028177A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- biostimulator
- liquid
- agricultural plants
- salicylic acid
- increasing resistance
- Prior art date
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
Definitions
- Liquid biostimulator increasing resistance of agricultural plants to stress conditions
- the subject of the invention is a liquid biostimulator, intended to be used in agricultural plants in order to increase their resistance to abiotic and biotic stress conditions.
- Yield capacity of agricultural plants is influenced by many abiotic factors related to physical and chemical properties of soil, such as porosity, mineral ingredient content, water content and aeration, acidity as well as factors influencing parts of the plant located above the ground, such as temperature, humidity, air composition or light intensity.
- An unfavourable state of one or more of the aforementioned factors, e.g. soil draught, too low or too high air temperature result in a stress of the agricultural plant, the most prevalent symptom of which is retarded or completely stopped growth as well as various development disturbances.
- Unfavourable biotic factors related to natural world, and in particular - pathogenic microbes and pests may also disturb natural growth and development of agricultural plants, contributing to lower quantity and quality of harvested crops.
- Salicylic acid is one of important compounds of plants responsible for numerous physiological and biochemical processes, such as e.g. uptake of mineral ingredients, permeability of cell membranes and mitochondrial respiration. Salicylic acid also acts as a signalling substance, inducing a number of processes in plants, leading to their increased tolerance to abiotic and biotic stress factors.
- biostimulator containing salicylic acid or its derivatives which would ensure complete dissolution of these compounds in water used in preparation of the spraying liquid and would facilitate its application onto plants, retention on their surface and penetration into plant cells.
- a number of soluble products containing salicylic or acetylsalicylic acid has been prepared, mainly intended for pharmaceutical and cosmetic uses. Unfortunately, many of these products do not find practical uses because of their toxicity to human beings and to environments (e.g. lithium salts of aspirine) because of adverse effects.
- a soluble form of salicylic acid intended for dermatological and cosmetic uses was obtained by mixing salicylic acid with organic solvents, including butyl alcohol, glycereth-26, glycereth-7 and a mixture of polyethylene glycols with molar mass of 200, 400 and 600 g.
- organic solvents including butyl alcohol, glycereth-26, glycereth-7 and a mixture of polyethylene glycols with molar mass of 200, 400 and 600 g.
- An international patent application WO 0126464 discloses plant photosynthesis stimulators containing salicylic acid derivatives.
- a Hungarian patent disclosure HU 209617 discloses plant protection measures and bioregulators containing salicylic acid derivatives.
- a Polish patent no 202519 discloses an agricultural plant protection preparation in the form of a homogenous aqueous solution containing ascorbic acid as an ingredient alleviating plant stress.
- the biostimulator according to the present invention is a liquid and water- soluble, homogenous mixture containing in its concentrated form: 1% to 40% wt. of salicylic acid or its derivative, in particular acetylsalicylic acid, 0.1% to 30% wt. of triethanolamine, 10% to 25% wt. of a non-ionic surfactantat selected from alkylpolyglycosides, and water. It was shown unexpectedly that even a small admixture of triethanoleamine enabled a homogenous formulation of the biostimulator to be obtained, resulted in complete dissolution of salicylic or acetylsalicylic acid in the formulation and ensured solution stability in the temperature range of -15 to +50° C for at least 360 days.
- Working concentrations of the biostimulator concentrate in the spraying liquid varies from 0.1% to 2% T Presence of a biodegradable surfactant selected from alkylpolyglycosides in the composition of the biostimulator largely facilitates retention of sprayed droplets on the surface of sprayed plants, ensures good coverage of said surface and increased penetration of salicylic acid or its derivative into plant cells. Use of a homogenous mixture of salicylic or acetylsalicylic acid and a non-ionic surfactant in the same package ensures significantly facilitated and accelerated preparation process of the spraying liquid. In contrast to other soluble formulations of salicylic acid or of its derivatives, the biostimulator according to the invention shows very favourable application-related properties, in addition to characteristics enabling its good biological action.
- the biostimulator obtained in Example 1 was leaf-applied to sugar beet as a bioassay crop cultivated under field conditions. The tests were performed in 2008 in the Experimental Station Stary Sielec comprising a part of Institute of Natural Fibres and Medicinal Plants in Poznan. The plot field experiment was the randomized block design with four replicates per treatment. The biostimulator was applied at the 8-leaf stage of sugar beet at two doses: 1.0 and 2.0 L/ha using a hydraulic sprayer delivering 200 L/ha of spray liquid in which working concentration of the biostimulator concentrate was 0.5% and 1%, respectively. A significant rainfall deficit occurred during the 2008 vegetation season, which resulted in a soil draught during the May- July period.
- the biostimulator comprising the subject of the present invention displayed a very positive influence on sugar beet growth and development as well as on its root and leaf yields.
- Data presented in Table 1 show that the biostimulator applied at the dosage of 1 L/ha increased the total sugar beet yield by 36.4%, yield of roots by 28.2%) and yield of leaves by 64.9% in comparison to the untreated control plants.
- Control - 1050 100 815 100 235 100
- Biostimulator 2.0 1365 130 1030 126 335 143
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A liquid, homogenous biostimulator useful for increasing resistance of agricultural plants to biotic and abiotic stress factors, containing salicylic acid or its derivative as its biologically active substance, as well as triethanolamine and a non-ionic surfactant selected from alkylpolyglycosides.
Description
Liquid biostimulator increasing resistance of agricultural plants to stress conditions
The subject of the invention is a liquid biostimulator, intended to be used in agricultural plants in order to increase their resistance to abiotic and biotic stress conditions.
Yield capacity of agricultural plants is influenced by many abiotic factors related to physical and chemical properties of soil, such as porosity, mineral ingredient content, water content and aeration, acidity as well as factors influencing parts of the plant located above the ground, such as temperature, humidity, air composition or light intensity. An unfavourable state of one or more of the aforementioned factors, e.g. soil draught, too low or too high air temperature result in a stress of the agricultural plant, the most prevalent symptom of which is retarded or completely stopped growth as well as various development disturbances. Unfavourable biotic factors related to natural world, and in particular - pathogenic microbes and pests may also disturb natural growth and development of agricultural plants, contributing to lower quantity and quality of harvested crops.
Agricultural plants produce many specific compounds they use as defence against stress factors, amongst other uses. Salicylic acid is one of important compounds of plants responsible for numerous physiological and biochemical processes, such as e.g. uptake of mineral ingredients, permeability of cell membranes and mitochondrial respiration. Salicylic acid also acts as a signalling substance, inducing a number of processes in plants, leading to their increased tolerance to abiotic and biotic stress factors.
Very low solubility of salicylic or acetylsalicylic acid in water is an important inconvenience, limiting practical use of these compounds on plants under agricultural conditions. This unfavourable physical property largely prevents correct preparation of a spraying liquid and its application on agricultural plants with commonly used hydraulic spraying equipment. In addition, droplets of the spraying liquid falling on hydrophobic plant surfaces are poorly retained therein, and salicylic or acetylsalicylic acid quickly crystallises after retention, limiting its penetration into plant cells, thus largely limiting its biostimulating activity. Thus, it
would be preferable to use such formulation of the biostimulator containing salicylic acid or its derivatives which would ensure complete dissolution of these compounds in water used in preparation of the spraying liquid and would facilitate its application onto plants, retention on their surface and penetration into plant cells.
A number of soluble products containing salicylic or acetylsalicylic acid has been prepared, mainly intended for pharmaceutical and cosmetic uses. Unfortunately, many of these products do not find practical uses because of their toxicity to human beings and to environments (e.g. lithium salts of aspirine) because of adverse effects.
In a solution known from the US patent disclosure No 5776431, acetylsalicylic acid soluble in water and stable during storage was obtained by combining it with a mixture of potassium citrate monohydrate and sodium citrate dihydrate.
In the US patent disclosure No 66689964, a soluble form of salicylic acid intended for dermatological and cosmetic uses was obtained by mixing salicylic acid with organic solvents, including butyl alcohol, glycereth-26, glycereth-7 and a mixture of polyethylene glycols with molar mass of 200, 400 and 600 g.
An international patent application WO 0126464 discloses plant photosynthesis stimulators containing salicylic acid derivatives.
A Hungarian patent disclosure HU 209617 discloses plant protection measures and bioregulators containing salicylic acid derivatives.
A Polish patent no 202519 discloses an agricultural plant protection preparation in the form of a homogenous aqueous solution containing ascorbic acid as an ingredient alleviating plant stress.
The biostimulator according to the present invention is a liquid and water- soluble, homogenous mixture containing in its concentrated form: 1% to 40% wt. of salicylic acid or its derivative, in particular acetylsalicylic acid, 0.1% to 30% wt. of triethanolamine, 10% to 25% wt. of a non-ionic surfactantat selected from alkylpolyglycosides, and water. It was shown unexpectedly that even a small admixture of triethanoleamine enabled a homogenous formulation of the biostimulator to be obtained, resulted in complete dissolution of salicylic or acetylsalicylic acid in the formulation and ensured solution stability in the temperature range of -15 to +50° C for at least 360 days. Working concentrations of the biostimulator concentrate in the spraying liquid varies from 0.1% to 2%T
Presence of a biodegradable surfactant selected from alkylpolyglycosides in the composition of the biostimulator largely facilitates retention of sprayed droplets on the surface of sprayed plants, ensures good coverage of said surface and increased penetration of salicylic acid or its derivative into plant cells. Use of a homogenous mixture of salicylic or acetylsalicylic acid and a non-ionic surfactant in the same package ensures significantly facilitated and accelerated preparation process of the spraying liquid. In contrast to other soluble formulations of salicylic acid or of its derivatives, the biostimulator according to the invention shows very favourable application-related properties, in addition to characteristics enabling its good biological action.
The invention is presented in the following examples, however, the examples do not exhaust all possible embodiments and applications thereof.
Example 1
250 g of triethanolamine, 200 g of acetylsalicylic acid, 250 g of a non-ionic surfactant selected from alkylpolyglycosides (commercial product GLUCOPON 650 EC by Cognis, Co. comprising a 50% aqueous solution of an alkylpolyglycoside surfactant) and 300 g of water were placed in a 1 L conical flask. The entire mixture was mixed at approximately 20°C for 10 minutes using a magnetic stirrer, until mixture ingredients formed a completely homogenous mixture. The biostimulator concentrate was obtained as a homogenous, clear solution of light yellow colour and density of 1.178 g/cm3, containing 20% wt. of pure acetylsalicylic acid, 25% wt. of pure triethanolamine, 12.5% wt. of a pure, non-ionic surfactant selected from alkylpolyglycosides, present in the Glucopon 650 EC commercial product, as well as 42.5% wt. of water. The obtained product with a code name "biostimulator" was used in field tests in order to evaluate its biostimulating activity.
Example 2
250 g of triethanolamine, 200 g of salicylic acid, 250 g of a non-ionic surfactant selected from alkylpolyglycosides (commercial product GLUCOPON 650 EC by Cognis, Co. comprising a 50% aqueous solution of an alkylpolyglycoside surfactant) and 300 g of water were placed in a 1 L conical flask. The entire mixture
was mixed at approximately 20°C for 10 minutes using a magnetic stirrer, until mixture ingredients formed a completely homogenous mixture. The biostimulator concentrate was obtained as a homogenous, clear solution of light yellow colour and density of 1.176 g/cm3, containing 20% wt. of pure salicylic acid, 25% wt. of pure triethanolamine, 12.5% wt. of a pure, non-ionic surfactant selected from alkylpolyglycosides, present in the Glucopon 650 EC commercial product, as well as 42.5% wt. of water.
Example 3
75 g of triethanolamine, 50 g of salicylic acid, 500 g of a non-ionic surfactant selected from alkylpolyglycosides (commercial product GLUCOPON 650 EC by Cognis, Co. comprising a 50% aqueous solution of an alkylpolyglycoside surfactant) and 375 g of water were placed in a 1 L conical flask. The entire mixture was mixed at approximately 20°C for 10 minutes using a magnetic stirrer, until mixture ingredients formed a completely homogenous mixture. The biostimulator concentrate was obtained as a homogenous, clear solution of light yellow colour, containing 5.0% wt. of pure acetylsalicylic acid, 7.5% wt. of pure triethanolamine, 25%) wt. of a pure, non-ionic surfactant selected from alkylpolyglycosides, present in the Glucopon 650 EC commercial product, as well as 62.5% wt. of water.
Example 4
The biostimulator obtained in Example 1 was leaf-applied to sugar beet as a bioassay crop cultivated under field conditions. The tests were performed in 2008 in the Experimental Station Stary Sielec comprising a part of Institute of Natural Fibres and Medicinal Plants in Poznan. The plot field experiment was the randomized block design with four replicates per treatment. The biostimulator was applied at the 8-leaf stage of sugar beet at two doses: 1.0 and 2.0 L/ha using a hydraulic sprayer delivering 200 L/ha of spray liquid in which working concentration of the biostimulator concentrate was 0.5% and 1%, respectively. A significant rainfall deficit occurred during the 2008 vegetation season, which resulted in a soil draught during the May- July period. Under such conditions, the biostimulator comprising the subject of the present invention displayed a very positive influence on sugar beet growth and development as well as on its root and
leaf yields. Data presented in Table 1 show that the biostimulator applied at the dosage of 1 L/ha increased the total sugar beet yield by 36.4%, yield of roots by 28.2%) and yield of leaves by 64.9% in comparison to the untreated control plants.
An increase of the biostimulator dose from 1 to 2 L/ha did not result in an additional, significant increase of yield of the tested crop.
T a b 1 e 1. Biostimulator efficiency in sugar beet cultivation
(Experimental Station Stary Sielec, IWNiRZ, 2008)
Yield of sugar beet
Treatments Dose total roots leaves
(L/ha) dt/ha % dt/ha % dt/ha %
Control - 1050 100 815 100 235 100
Biostimulator 1.0 1432 136 1045 128 387 165
Biostimulator 2.0 1365 130 1030 126 335 143
Least significant difference
64 63 31
(LSD, p = 0.05)
Claims
1. A liquid, homogenous biostimulator useful for increasing resistance of agricultural plants to biotic and abiotic stress factors characterised in that it contains 1% to 40% wt. of salicylic acid or its derivatives, 0.1%> to 30%> of triethanolamine, 10%> to 25% wt. of non-ionic surfactant selected from alkylpolyglycosides and water, whilst working concentration of said biostimulator concentrate in the spraying liquid is 0.1%> to 2%.
2. Liquid, homogenous biostimulator according to Claim 1 characterised in that it contains acetylsalicylic acid as a salicylic acid derivative.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PLP409229 | 2014-08-21 | ||
| PL409229A PL230888B1 (en) | 2014-08-21 | 2014-08-21 | Liquid biostimulator increasing resistance of cultivated plants to stress conditions |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2016028177A1 true WO2016028177A1 (en) | 2016-02-25 |
Family
ID=54065425
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/PL2015/050031 WO2016028177A1 (en) | 2014-08-21 | 2015-07-24 | Liquid biostimulator increasing resistance of agricultural plants to stress conditions |
Country Status (2)
| Country | Link |
|---|---|
| PL (1) | PL230888B1 (en) |
| WO (1) | WO2016028177A1 (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL202519A1 (en) | 1977-11-28 | 1978-10-09 | Akad Gorniczo Hutnicza | METHOD OF INTENSIFYING THE WORK OF A FILTER WITH POROUS FILLING |
| HU209617B (en) | 1989-06-14 | 1994-09-28 | Basf Ag | Herbicidal and bioregulatory compositions containing salicylic aldehyde and salicylic acid derivatives and sulfur analogues, thereof, as well as process for producing the active ingredients |
| WO1995008982A1 (en) * | 1993-09-28 | 1995-04-06 | Isp Investments Inc. | Uv protective film-forming composition |
| WO1997047171A1 (en) * | 1996-06-10 | 1997-12-18 | Smithkline Beecham Plc | Skin wash composition |
| US5776431A (en) | 1997-03-26 | 1998-07-07 | Galat; Alexander | Water-soluble aspirin composition |
| WO2000032048A1 (en) * | 1998-12-03 | 2000-06-08 | Redox Chemicals, Inc. | Novel methods of protecting plants from pathogens |
| WO2001026464A1 (en) | 1999-10-08 | 2001-04-19 | Mcgill University | Salicyl acid and related phenolic compounds for increasing photosynthesis in plants |
| US6669964B2 (en) | 2001-06-06 | 2003-12-30 | Lipo Chemicals, Inc. | Composition for solubilizing salicylic acid |
-
2014
- 2014-08-21 PL PL409229A patent/PL230888B1/en unknown
-
2015
- 2015-07-24 WO PCT/PL2015/050031 patent/WO2016028177A1/en active Application Filing
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL202519A1 (en) | 1977-11-28 | 1978-10-09 | Akad Gorniczo Hutnicza | METHOD OF INTENSIFYING THE WORK OF A FILTER WITH POROUS FILLING |
| HU209617B (en) | 1989-06-14 | 1994-09-28 | Basf Ag | Herbicidal and bioregulatory compositions containing salicylic aldehyde and salicylic acid derivatives and sulfur analogues, thereof, as well as process for producing the active ingredients |
| WO1995008982A1 (en) * | 1993-09-28 | 1995-04-06 | Isp Investments Inc. | Uv protective film-forming composition |
| WO1997047171A1 (en) * | 1996-06-10 | 1997-12-18 | Smithkline Beecham Plc | Skin wash composition |
| US5776431A (en) | 1997-03-26 | 1998-07-07 | Galat; Alexander | Water-soluble aspirin composition |
| WO2000032048A1 (en) * | 1998-12-03 | 2000-06-08 | Redox Chemicals, Inc. | Novel methods of protecting plants from pathogens |
| WO2001026464A1 (en) | 1999-10-08 | 2001-04-19 | Mcgill University | Salicyl acid and related phenolic compounds for increasing photosynthesis in plants |
| US6669964B2 (en) | 2001-06-06 | 2003-12-30 | Lipo Chemicals, Inc. | Composition for solubilizing salicylic acid |
Also Published As
| Publication number | Publication date |
|---|---|
| PL409229A1 (en) | 2016-02-29 |
| PL230888B1 (en) | 2018-12-31 |
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