WO2016017950A1 - Appareil de mesure de micro-organisme aérien et son procédé de mesure - Google Patents

Appareil de mesure de micro-organisme aérien et son procédé de mesure Download PDF

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Publication number
WO2016017950A1
WO2016017950A1 PCT/KR2015/006908 KR2015006908W WO2016017950A1 WO 2016017950 A1 WO2016017950 A1 WO 2016017950A1 KR 2015006908 W KR2015006908 W KR 2015006908W WO 2016017950 A1 WO2016017950 A1 WO 2016017950A1
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WIPO (PCT)
Prior art keywords
filter
unit
microbial
particles
light
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PCT/KR2015/006908
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English (en)
Korean (ko)
Inventor
박철우
이성화
Original Assignee
주식회사 엘지전자
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Application filed by 주식회사 엘지전자 filed Critical 주식회사 엘지전자
Priority to CN201580040878.0A priority Critical patent/CN106662576B/zh
Publication of WO2016017950A1 publication Critical patent/WO2016017950A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements

Definitions

  • the present invention relates to an airborne microbial measurement apparatus and a measuring method thereof.
  • the biological particles suspended in the sample gas are collected on a solid or liquid surface suitable for propagation, incubated in a suitable temperature and humidity environment for a certain period of time, and then collected in the colony water on the surface. And culture methods for obtaining and staining using a fluorescence microscope after staining.
  • ATP bioluminescence method uses the principle that ATP (adenosine triphosphate) and luciferin / luciferase react to make light, which is a series of ATP scavenging process, ATP extraction and emission measurement The process has been reduced to about 30 minutes, allowing for quick work.
  • FIG 9 shows a configuration of an electrostatic precipitator provided in a conventional particle sorting apparatus.
  • the conventional electrostatic precipitator 1 includes a charging line 3 (discharge electrode) disposed between two collecting plates 2 on both sides and the collecting plate 2 on both sides.
  • the charging line 3 When a high voltage is applied to the charging line 3, corona discharge is generated, and ions generated at this time are charged with predetermined particles in the gas.
  • the charged particles may be collected by being moved by an electric force to the collecting electrode, that is, the collecting plate 2.
  • the electrostatic precipitator 1 can be understood as a dust collector capable of collecting certain particles using an electrostatic principle.
  • the predetermined particles may include foreign matter such as dust, or airborne microorganisms.
  • the conventional airborne microbial measurement apparatus the electrostatic precipitator and a collecting rod for collecting the airborne microorganisms collected in the collecting plate.
  • the conventional airborne microbial measurement apparatus is configured to collect or sample the airborne microorganisms by manually contacting the collecting rod to the collecting plate when the airborne microorganisms are collected on the collecting plate by driving the electrostatic precipitator.
  • the collected suspended microorganisms are reacted with a reagent to emit light, and the emitted light is detected to measure the concentration of the microorganisms.
  • a collection rod must be separately prepared and the user must go through a process of collecting the airborne microorganisms collected on the collecting plate by using the collection rod. there was.
  • the present invention has been proposed to solve such a problem, and an object of the present invention is to provide an airborne microbial measurement apparatus and a method for measuring the airborne microorganisms present in the gas phase.
  • Airborne microbial measurement apparatus including an inlet for the inlet of air and a nozzle unit provided on one side of the inlet; A microbial particle flow path through which the microbial particles having passed through the internal flow path of the nozzle unit flow; A driving device for generating a flow of the microbial particles; A collecting device in communication with the microbial particle flow passage and including a filter unit for collecting the microbial particles; A luminescence measuring device for sensing an amount or intensity of light generated from the microbial particles collected in the filter unit; And a sterilization apparatus provided on one side of the filter part to sterilize the filter part.
  • a housing provided on one side of the collecting device, the housing for receiving the light emission measuring device and sterilization device is further included.
  • the suction unit is formed inside the housing, and guides the flow of the microbial particles to the filter unit by the driving of the drive device.
  • the light emission measuring device and the sterilizing device is characterized in that it is installed on both sides of the suction unit.
  • the collecting device may include a filter case accommodating the filter part and forming a filter hole communicating with the microbial particle flow path, wherein at least a portion of the filter part is exposed to the outside through the filter hole. do.
  • the filter case and the filter unit is characterized in that the rotatable.
  • the filter hole is characterized in that it can be arranged in a position corresponding to any one of the suction unit, the light receiving unit and the sterilizer.
  • the filter hole is characterized in that it can be arranged in a position corresponding to the suction unit, the sterilizer and the light receiving unit in order.
  • the filter hole may include a plurality of filter holes spaced apart from each other, and the spaced distances of the plurality of filter spaces correspond to spaced distances of the suction unit, the sterilizer, and the light receiving unit.
  • control unit for controlling the sterilization apparatus is further included, wherein the control unit is characterized in that before operating the microorganism particles are collected in the filter unit, by operating the sterilization unit to remove contaminants in the filter unit.
  • the apparatus may further include a controller configured to control the light emission measuring device, wherein the control unit first operates the light emission measuring device before the microorganism particles are collected in the filter unit, and the microorganism particles may be disposed in the filter unit. After being collected, the luminescence measuring device is operated for a second time.
  • the drive device also includes an air pump device.
  • the sterilizing apparatus includes an ultraviolet LED device or an ionizer.
  • the light emission measuring device the light receiving unit for collecting light
  • a reflection induction device that guides the light to the light receiving portion and induces total reflection or diffuse reflection of the light.
  • the reflection induction device includes a film part or a coating part.
  • the display unit for displaying the concentration of the microorganisms detected by the luminescence measuring device is further included.
  • the concentration of the microorganisms displayed on the display unit is high, it is characterized in that to transmit information about the concentration of the microorganisms to the household appliances for purifying the air.
  • a method of measuring suspended microorganisms includes: performing a first operation of a filter driving unit, placing a sterilizer in one region of a filter unit, and operating the sterilizer; Performing a second operation of the filter driver to position the light receiver in one region of the filter unit and performing a first operation of the light receiver; Performing a third operation of the filter driving unit to position the suction unit through which the microbial particles can flow in one region of the filter unit; The driving device is driven, and the microbial particles in the air are separated, and the separated microbial particles are collected by the suction unit to the filter unit.
  • the method may further include calculating a microbial emission amount by subtracting the detected first emission amount by performing the first operation of the light receiver from the second emission amount detected by performing the second operation of the light receiver.
  • the airborne microorganisms in the air to the virtual impactor (virtual impactor) structure without the user need to manually sample the suspended microorganisms collected on the collecting plate It can be separated automatically by the particle sorting process is easy and takes less time.
  • the reference light emission value can be taken into consideration,
  • the advantage is that the concentration can be calculated.
  • the filter unit can be located on one side of the suction unit, the light receiving unit or the sterilization apparatus disposed side by side inside the second housing, so that the sterilization of the filter unit and the concentration measurement of the microorganisms
  • the advantage is that it can be done continuously.
  • the luminescence measurement process may be easily performed.
  • the virtual impactor structure may effectively separate the main flow having small particles and the sub flow having large particles.
  • a fan as a driving part on the main flow side where the pressure loss is relatively small
  • a low flow pump as a driving part on the sub flow side where the pressure loss is relatively large
  • the display unit for displaying the information on the microbial concentration based on the amount of light emitted by the light emitting device is further provided, and when the microbial concentration is higher than the set concentration may be displayed on the display to warn, the user convenience is increased Can be.
  • FIG. 1 is a perspective view showing the configuration of a suspended microbial measurement apparatus according to an embodiment of the present invention.
  • FIG. 2 is a cross-sectional view taken along line II ′ of FIG. 1.
  • FIG. 3 is a cross-sectional view taken along line II-II 'of FIG. 1.
  • Figure 4 is a schematic diagram showing the internal configuration of the airborne microbial measurement apparatus according to an embodiment of the present invention.
  • FIG. 5 is a view schematically showing a configuration of a nozzle unit according to an embodiment of the present invention.
  • Figure 6 is a block diagram showing the configuration of a suspended microbial measurement apparatus according to an embodiment of the present invention.
  • FIG. 7 is a flow chart showing a measuring method of a suspended microbial measurement apparatus according to an embodiment of the present invention.
  • FIG. 8a to 8e is a schematic view showing the action of the airborne microbial measurement apparatus according to an embodiment of the present invention.
  • FIG. 9 is a view showing the configuration of the electrostatic precipitator provided in the conventional airborne microbial measurement apparatus.
  • FIG. 1 is a perspective view showing the configuration of a suspended microbial measurement apparatus according to an embodiment of the present invention
  • Figure 2 is a cross-sectional view taken along the line II 'of Figure 1
  • Figure 3 is a line II-II' of FIG. It is an incision section.
  • the airborne microbial measurement apparatus includes a base 20 and a plurality of devices installed above the base 20.
  • the plurality of devices include a particle sorting device 100 for sucking air to separate the airborne microorganisms and a collecting device 200 for collecting the airborne microorganisms separated from the particle sorting device 100.
  • the plurality of devices are provided on one side of the collecting device 200 and electrically connected to the light emission measuring device 300 and the light emission measuring device 300 for detecting the amount or intensity of light generated from the suspended microorganism.
  • the control device 400 is further included.
  • the light emission measuring apparatus 300 includes a light receiving unit 320 for collecting light.
  • the control device 400 includes a PCB 410 on which a plurality of circuit components are installed, and a display unit 420 installed on the PCB 410 to display information on the concentration of suspended microorganisms.
  • the particle sorting apparatus 100 includes a first housing 110 forming a predetermined inner space and an upper surface portion 112 coupled to an upper portion of the first housing 110.
  • a plurality of slits 121 are formed as “air inlets” through which air existing outside the particle sorting apparatus 100 is sucked.
  • the width of the slit 121 may be in the range of several millimeters (mm).
  • a plurality of slits 121 are formed on the upper surface part 112
  • the resistance force of air introduced through the slits 121 that is, the differential pressure inside and outside the slits 121 is small. Therefore, a sufficient flow rate of air introduced through the plurality of slits 121 may be secured.
  • a nozzle unit 120 through which air introduced through the slit 121 passes is provided inside the first housing 110. That is, the nozzle unit 120 may be installed in the inner space of the first housing 110. In addition, the nozzle unit 120 is spaced downward from the slit 121 and extends downward.
  • the nozzle unit 120 may be provided in plurality, corresponding to the number of the plurality of slits 121, may be spaced apart from each other. For example, as illustrated in FIG. 2, the plurality of nozzle units 120 may be spaced apart from each other in the horizontal direction.
  • the nozzle unit 120 includes an inner passage 125 through which floating microbial particles in the air introduced into the first housing 110 through the slit 121 flow.
  • the internal passage 125 forms an internal space of the nozzle unit 120.
  • the inner passage 125 defines an end portion of the nozzle unit 120 and has an inlet portion 125a through which floating microorganisms flow into the inner passage 125.
  • the inlet 125a is formed at the upper end of the inner passage 125.
  • Airborne microbial particles in the air introduced through the slit 121 flows through the internal passage 125 through the inlet portion 125a, and the air particles from which the airborne microbial particles are separated are separated from the internal passage 125.
  • the outer space flows and passes through the air particle passage 129.
  • the inner passage 125 defines the other end of the nozzle portion 120, and the outlet portion 125b for allowing the floating microbial particles flowing through the inner passage 125 to be discharged from the nozzle portion 120. Is formed. In one example, the outlet 125b is formed at the lower end of the internal passage 125.
  • the air particle passage 129 may be referred to as a first flow passage or a main flow passage, and the microbial particle passage 127 may be referred to as a second flow passage or a sub flow passage.
  • the lower end of the nozzle unit 120 is provided with a partition plate 126 that partitions the air particle passage 129 and the microbial particle passage 127.
  • the lower end portion of the nozzle portion 120 that is, the outlet portion 125b is coupled to the partition plate 126.
  • the outlet portion 125b may be formed in the partition plate 126.
  • One side of the first housing 110 is provided with a second housing 130 in which the light receiving unit 320 and the sterilizer 330 are installed.
  • the microbial particle flow path 127 extends from one side of the partition plate 126 toward the collecting device 200, and the inner space of the second housing 130 may extend at least a portion of the microbial particle flow path 127. Can be formed.
  • a filter case 210 in which the filter unit 220 is accommodated and a plurality of filter holes 215 formed in the filter case 210 are formed.
  • At least a portion of the filter case 210 is inserted into the second housing 130.
  • the second housing 130 may be disposed to surround the upper and lower portions of at least a portion of the filter case 210.
  • the filter case 210 may have an approximately semicircular cross section.
  • the plurality of filter holes 215 may be spaced apart from each other along the edge of the filter case 210 may be disposed in the circumferential direction. The distances between the plurality of filter holes 215 may be the same.
  • the filter unit 220 may be exposed to the outside through the plurality of filter holes 215.
  • the microbial particles flowing through the microbial particle flow path 127 may be collected in the filter unit 220 through any one of the filter holes 215 of the plurality of filter holes 215.
  • the filter unit 220 may be installed to be fixed to the inside of the filter case 210.
  • the filter case 210 may be rotatably provided.
  • the filter driver 250 includes a motor capable of forward or reverse rotation.
  • the motor may include a step motor.
  • a rotating shaft 255 extends from the filter driver 250 to the filter case 210.
  • the rotating shaft 255 is rotated, and the filter case 210 may be rotated clockwise or counterclockwise by the rotating shaft 255.
  • the filter unit 220 may be rotated together with the filter case 210.
  • one filter hole 215 communicates with the microbial particle flow path 127. Therefore, the microbial particles flowing through the microbial particle flow path 127 are collected in the filter unit 220 through the one filter hole 215.
  • one region of the filter unit 220 in which the microbial particles are collected may correspond to the region exposed to the microbial particle passage 127 by the one filter hole 215.
  • the filter case 210 and the filter unit 220 are rotated, another filter hole 215 communicates with the microbial particle flow path 127, and the one filter hole 215 is moved in position to measure the emission.
  • the light receiving unit 320 or the sterilizer 330 of the device may be located on one side.
  • the pump device 360 On one side of the collecting device 200, the pump device 360 as a "drive device” that is driven for the flow of microbial particles and the pump connection portion 350 extending from the second housing 130 to the pump device 360 ) Is provided.
  • the pump device 360 may include an air pump.
  • a suction part 310 communicating with the pump connection part 350 is included inside the second housing 130.
  • the suction part 310 is formed in the second housing 130, and the suction force of the pump device 360 may act.
  • the suction part 310 may be formed by cutting or penetrating at least a portion of the second housing 130.
  • the suction part 310 may be formed on one side of the filter case 210 and the upper side of the drawing.
  • the pump device 360 when the pump device 360 is driven, air flow in the microbial particle flow path 127 is generated, and the air flow passes through the filter part 220 through the suction part 310. In this process, the microbial particles may be collected in the filter unit 220. The air flow after the microbial particles are separated may flow to the pump device 360 via the pump connection unit 350.
  • the pump connection portion 350 includes a cyclone portion 351 having a reduced flow cross-sectional area from the second housing 130 toward the pump device 360.
  • the air flow may increase the flow rate while passing through the cyclone portion 351 and may be introduced into the pump device 360.
  • the pump device 360 may be understood as a device that is more advantageous than a fan to secure a predetermined suction flow rate even if a pressure loss occurs. Therefore, by generating the particle flow in the microbial particle flow path 127 using the pump device 360, even if a pressure loss occurs in the nozzle unit 120 or the filter unit 220, the suction efficiency can be improved. have.
  • the flow rate of the microbial particle flow path 127 is relatively small, a low flow rate pump may be applied to the air pump. Therefore, the phenomenon that the floating microorganism measuring device becomes large or heavy can be prevented.
  • the light emission measuring apparatus 300 includes a light receiving unit 320 for the microbial particles located at one side of the collecting device 200.
  • the light receiving unit 320 may be located inside the second housing 130. In addition, the light receiving unit 320 may be spaced apart from one side of the suction unit 310.
  • the light receiver 320 may include a relatively inexpensive LED and CCD camera.
  • the LED may be a blue LED.
  • the light emission measuring device 300 may be provided at one side of the light receiving unit 320 to provide a light receiving unit guide device for guiding light to the light receiving unit 320.
  • the light receiver guide device may include a reflection induction device for inducing total reflection or diffuse reflection of light.
  • the reflection induction apparatus includes a film portion or a coating portion having a reflection function.
  • the spaced distance between the suction part 310 and the light receiving part 320 may correspond to the distance between one filter hole among the plurality of filter holes 215 and another filter hole. Therefore, when the one filter hole is disposed at a position corresponding to the suction part 310, the other filter hole may be disposed at a position corresponding to the light receiving part 320.
  • the one filter hole is disposed at a position at which the flow force through the suction unit 310 can act, and the other filter hole has the light emission amount of the filter unit 220 exposed through the other filter hole.
  • the light receiver 320 may be disposed at a position that may act on the light receiver 320.
  • the filter case 210 When the filter case 210 is rotated after the microbial particles are collected in the filter unit 220 through one filter hole among the plurality of filter holes 215, the one filter hole faces the light receiving unit 320. Can be placed in.
  • the light receiver 320 may detect the amount or intensity of light generated from the microbial particles of the filter unit 220.
  • the airborne microbial measurement apparatus further includes a sterilization apparatus 330 for sterilizing contaminants present in the filter unit 220.
  • the sterilizer 330 may include an ultraviolet light emitting device or an ionizer.
  • the ultraviolet light emitting device includes an ultra violet-light emitting diode (LED).
  • the sterilizer 330 may be located inside the second housing 130.
  • the sterilizer 330 may be spaced apart from the other side of the suction part 310. That is, the light receiving part 320, that is, the light emission measuring device 300 and the sterilizing device 330 may be installed at both sides of the suction part 310.
  • the spaced distance between the suction unit 310 and the sterilization apparatus 330 may correspond to a distance between one filter hole and the other filter hole among the plurality of filter holes 215. Therefore, when the one filter hole is disposed at a position corresponding to the suction unit 310, the other filter hole may be disposed at a position corresponding to the sterilization apparatus 330.
  • the one filter ball is disposed at a position at which the flow force through the suction part 310 can act, and the other filter ball is disposed on the filter part 220 exposed through the other filter ball.
  • 330 may be placed in a position to act.
  • the suction part 310, the light receiving part 320, and the sterilizing device 330 may be spaced apart from each other to correspond to a shape in which the plurality of filter holes 215 are disposed.
  • the plurality of filter holes 215 are spaced apart along the circumference of the filter case 210, and the suction part 310, the light receiving part 320, and the sterilizer 330 are the plurality of filters.
  • the ball 215 may be disposed at a position corresponding to each filter hole 215.
  • the one filter hole 215 or the filter unit 220 from the dissolving agent supply device 370 for supplying a dissolution reagent to the filter unit 220 and the dissolving agent supply device 370 It further includes a supply passage 375 extending to.
  • the lysis reagent is understood as a soluble agent for lysing the cells (or cell walls) of the suspended microorganisms collected in the filter unit 220.
  • ATP is extracted.
  • a light emitting material may be applied to the filter unit 220.
  • the light emitting material is understood as a material for generating light by reacting with ATP (Adenosine Triphosphate, Adenosine Triphosphate) of the microbial particles extracted by the dissolution reagent.
  • the luminescent material includes luciferin and luciferase.
  • the luciferin is activated by ATP present in the lysed cells and is converted into active luciferin, and the active luciferin is oxidized by the action of luciferase, a light-emitting enzyme, to be oxidized luciferin, which emits light by converting chemical energy into light energy. .
  • a relatively small particle separated from the inlet side of the nozzle unit 120 for example, an air particle flow path 129 through which air particles flow is formed. Particles of the air particle flow path 129 is formed smaller than particles of the microbial particle flow path 127. However, the flow amount of the air particle flow path 129 may be greater than the flow amount of the microbial particle flow path 127.
  • the air particle flow path 129 is separated from the microbial particle flow path 127 by the partition plate 126 and extends toward the blowing fan 150.
  • the blowing fan 150 is a driving device for generating a flow of the air particle flow path 129, for example, may be accommodated in the fan housing 155.
  • the fan housing 155 is disposed under the first housing 110.
  • the blower fan 150 is understood as a device capable of ensuring a sufficient flow rate as compared to the air pump when the pressure loss is small. Therefore, since the blowing fan 150 is provided in a passage having a low pressure loss, such as the air particle passage 129, there is an effect that sufficient air particle flow (main flow) can be generated.
  • Figure 4 is a schematic diagram showing the internal configuration of the airborne microbial measurement apparatus according to an embodiment of the present invention
  • Figure 5 is a view schematically showing the configuration of the nozzle unit according to an embodiment of the present invention. 4 and 5, the operation of the airborne microbial measurement apparatus according to an embodiment of the present invention will be briefly described.
  • air (A in FIG. 5) existing outside the airborne microbial measurement apparatus 10 is formed into a plurality of slits 121 of the upper surface part 112. It is introduced into the first housing 110 through the.
  • the flow velocity may be increased by a narrow passage cross-sectional area.
  • Floating microbial particles having a relatively large amount of air particles passing through the plurality of slits 121 are introduced into the internal passage 125 through the inlet portion 125a of the nozzle unit 120 (FIG. 5C). .
  • the suspended microbial particles are discharged from the internal passage 125 through the outlet portion 125b, and then flow through the microbial particle passage 127.
  • air particles having relatively small particles in the air passing through the plurality of slits 121 do not flow to the inner passage 125 while the traveling direction is bent, and is along the outer space of the nozzle unit 120. It will flow (B of FIG. 5).
  • the air particles flow through the air particle passage 129 to pass through the blowing fan 150.
  • relatively large floating microbial particles are introduced into the internal passage 125 through the inlet portion 125a, and relatively small air particles are introduced into the slit (
  • the stream line may be bent through the spaced space between 121 and the inlet 125a.
  • Such a particle classification structure may be referred to as a virtual impactor structure.
  • the floating microbial particles and air particles may be easily classified by applying the virtual impactor structure.
  • the floating microbial particles flowing through the microbial particle flow path 127 flow to the collecting device 200, and pass through the filter unit 220 via one filter hole 215 of the suction part 310 and the filter case 210. Can be collected in one area of
  • the dissolution reagent is supplied to the filter unit 220 from the solvent supply device 370.
  • the microbial particles collected in the filter unit 220 may be dissolved by the dissolution reagent to extract ATP, and then react with the light emitting material applied to the filter unit 220.
  • the filter driving unit 250 is driven to rotate the filter case 210, whereby the one filter hole 215 is positioned to face the light receiving unit 320.
  • the light receiver 320 may detect an amount or intensity of light generated from the microbial particles collected by the filter unit 220.
  • the light may be generated during the reaction between the ATP and the light emitting material of the microbial particles.
  • one region of the filter part 220 in which the microbial particles are collected may be moved to face the light receiving part 320.
  • the filter case 210 and the filter unit 220 are rotatably provided, so that the microbial collection and light emission process can be automatically performed.
  • the sterilizer 330 may be operated to sterilize the filter unit 220 before the microbial particles are collected in the filter unit 220.
  • the light receiving unit 320 may be operated to detect the amount of light emitted from the filter unit 220 before the microbial particles are collected in the filter unit 220.
  • the light emission amount at this time may be referred to as "reference light emission amount" in that it provides reference information on the light emission amount when the microbial particles are collected later.
  • Figure 6 is a block diagram showing the configuration of a suspended microbial measurement apparatus according to an embodiment of the present invention.
  • the airborne microbial measurement apparatus 10 includes a pump device 360 for generating a floating microbial particle flow and a blowing fan 150 for generating an air particle flow.
  • the suspended microbial measuring device 10 a filter driving unit 250 for rotating the filter case 210 and the filter unit 220 and a dissolving agent supply device 370 for supplying a dissolution reagent to the filter unit 220 ) Is further included.
  • the airborne microbial measurement apparatus 10 includes a display unit 420 that displays information on the concentration of airborne microbial particles collected by the filter unit 220.
  • the display unit 420 may include a lighting device displayed in a different color according to the concentration value of the suspended microbial particles.
  • the lighting apparatus may include a first lighting unit displaying green when the concentration of the floating microbial particles is low, a second lighting unit displaying yellow when the concentration is about a medium value, and a third display unit displaying red when the concentration is high.
  • the lighting unit may be included.
  • the first to third lighting units may be configured as one lighting unit.
  • a timer for accumulating the elapsed time of the light receiving unit 320 for detecting the amount of light emitted from the microbial particles collected in the filter unit 220 and the collection process of the microbial particles and the dissolution reagent supply process 460 is included.
  • Information detected by the light receiving unit 320 or the timer 460 may be transmitted to the control unit 450, and the control unit 450 may include the pump device 360 and a blower fan based on the transferred information.
  • the control unit 450 may include the pump device 360 and a blower fan based on the transferred information.
  • the operation of the filter driver 250, the solvent supply device 370 and the display unit 420 may be controlled.
  • the airborne microbial measurement apparatus 10 further includes a sterilizer 330 for removing contaminants present in the filter unit 220.
  • a sterilizer 330 for removing contaminants present in the filter unit 220.
  • the airborne microbial measurement apparatus 10 further includes a memory unit 470 that stores information regarding the operation of the light emission measurement apparatus, that is, the light receiving unit 320.
  • the light receiver 320 may perform a first operation before the microbial particles are collected and a second operation after the microbial particles are collected.
  • the first operation is an operation for detecting the amount of light emitted by the light around the collecting device 200 and is understood as an operation for detecting the reference amount of light.
  • Information on the reference light emission amount according to the first operation may be stored in the memory unit 470.
  • the information about the reference light emission amount may be considered to calculate the light emission amount detected after the second operation.
  • the reference light emission amount may be referred to as a “first light emission amount” and the light emission amount detected after the second operation may be referred to as a “second light emission amount”.
  • the concentration value of the microorganisms collected in the filter part may be calculated based on a value obtained by subtracting the reference emission amount from the second emission amount.
  • FIG. 7 is a flow chart showing a measuring method of a floating microbial measuring apparatus according to an embodiment of the present invention
  • Figures 8a to 8e is a schematic diagram showing the action of the floating microbial measuring apparatus according to an embodiment of the present invention.
  • the semicircular filter case 210 extends from side to side and the suction unit 310 and the light receiving unit on one side of the filter case 210. It indicates that the position of the 320 and the sterilizer 330 is relatively displayed.
  • the filter driving unit 250 when the power of the floating microorganism measuring device 10 is turned on, the filter driving unit 250 performs a first operation.
  • the first operation of the filter driving unit 250 is an operation of rotating in a reverse direction by a first set angle to sterilize a filter hole 215a (see FIG. 8A) for opening a region of the filter unit 220 in which microorganisms are to be collected. It is understood as an operation for moving to one side of 300.
  • the filter hole 215a may be referred to as a "collection filter hole".
  • the reverse direction may correspond to a direction in which the filter case 210 moves to the left side with reference to FIG. 8A.
  • the first set angle is understood as an angle at which the filter case 210 can be rotated by a distance (separation distance) between one filter hole and another filter hole closest to the one filter hole.
  • Such "reverse first rotation of the set angle” may be referred to as “-1 rotation” (S12).
  • FIG. 8A shows a basic arrangement of the floating microbial measurement apparatus 10, that is, the state when the power of the floating microbial measurement apparatus 10 is turned on.
  • the suction unit 310 is located on one side of the collecting filter hole (215a) of the filter case 210, the sterilization device is located on one side of the other filter hole.
  • the light receiving unit 320 may be positioned outside the plurality of filter holes.
  • the filter case 210 When the first operation of the filter driver 250 is performed, the filter case 210 is rotated and disposed as shown in FIG. 8B, and one side of the collecting filter hole 215a of the filter case 210.
  • the sterilization apparatus 330 In the sterilization apparatus 330 is located. That is, the sterilizer 330 is disposed at a position capable of sterilizing one region of the filter unit 220 through the collection filter hole 215a (see FIG. 8B).
  • the sterilizer 330 may irradiate a light source toward one region of the filter unit 220 (S13).
  • the filter driver 250 After the sterilizer 330 is operated, the filter driver 250 performs a second operation.
  • the second operation of the filter driving unit 250 is an operation of rotating the collecting filter hole 215a to one side of the light receiving unit 320 as an operation of rotating the second predetermined angle in the forward direction.
  • the forward direction may correspond to a direction in which the filter case 210 moves to the right with reference to FIG. 8B.
  • the second set angle is understood as an angle through which the filter case 210 can be rotated by twice the separation distance.
  • This "second forward rotation of the set angle” may be referred to as “+2 rotation” (S14).
  • the filter case 210 is arranged as shown in FIG. 8C, and at one side of the collection filter hole 215a, the light receiving unit 320 is positioned. do. That is, the light receiving unit 320 is disposed at a position capable of detecting the amount of light emitted from one region of the filter unit 220 through the collecting filter hole 215a (see FIG. 8C).
  • the suction part 310 is positioned at one side of the other filter hole among the plurality of filter holes, and the sterilizer 330 may be positioned at one side of the other filter hole. This is due to the fact that each spaced distance of the suction unit 310, the light receiving unit 320, and the sterilizer 330 corresponds to the spaced distance of the plurality of filter holes.
  • the light receiving unit 320 that is, the light emission measuring device performs a first operation to detect the amount of light emitted from the filter unit 220.
  • the light emission amount detected by the first operation of the light receiver 320 is a light emission amount that can be basically sensed by the filter unit 220 before the microbial particles are collected, and is referred to as a "reference light emission amount (first light emission amount)" value.
  • the information about the reference emission amount may be stored in the memory unit 470 (S15).
  • the filter driver 250 After the first operation of the light receiver 320, the filter driver 250 performs a third operation.
  • the third operation of the filter driving part 250 is an operation of rotating the collecting filter hole 215a to one side of the suction part 310 as an operation of rotating the third set angle in the reverse direction.
  • the reverse direction may correspond to a direction in which the filter case 210 moves to the left side with reference to FIG. 8C.
  • the third set angle is understood as an angle at which the filter case 210 may be rotated by the separation distance.
  • This "reverse third rotation of the set angle” may be referred to as “-1 rotation” (S16).
  • the filter case 210 is arranged as shown in FIG. 8D, and the suction unit 310 is positioned at one side of the collection filter hole 215a. Done. That is, the suction part 310 is disposed at a position where the microbial particles can flow to one region of the filter part 220 through the suction part 310 and the collecting filter hole 215a.
  • the blower fan 150 and the pump device 360 operate to generate a main flow to the blower fan 150 and a subflow to the pump device 360.
  • external air of the floating microorganism measuring device 10 is introduced into the first housing 110 through the plurality of slits 121.
  • This collection process may be performed during the first set time.
  • the elapsed time is accumulated by the timer 460, and the controller 450 recognizes whether the first set time has elapsed (S18).
  • the dissolving agent supply device 370 operates to supply the dissolving reagent to the filter unit 220.
  • the lysis reagent is supplied to the filter unit 220 for a second set time, and the operation of the solvent supply device 370 is stopped when the second set time elapses.
  • the dissolution reagent dissolves the microbial particles collected in the filter unit 220 to extract ATP, and the extracted ATP reacts with the light emitting material applied to the filter unit 220 to emit a predetermined light (S19, S20).
  • the filter driver 250 performs a fourth operation.
  • the fourth operation of the filter driving unit 250 is an operation of rotating the collecting filter hole 215a to one side of the light receiving unit 320 as an operation of rotating the fourth set angle in the forward direction.
  • the forward direction may correspond to a direction in which the filter case 210 moves to the right with reference to FIG. 8D.
  • the fourth set angle is understood as an angle at which the filter case 210 can be rotated by the separation distance.
  • This "fourth set-angle rotation in the forward direction” may be referred to as “+1 rotation” (S21).
  • the filter case 210 is arranged as shown in FIG. 8E, and at one side of the collection filter hole 215a, the light receiving unit 320 is positioned. do. That is, the light receiving unit 320 is disposed at a position capable of detecting the amount of light emitted from one region of the filter unit 220 in which the microbial particles are collected through the collecting filter hole 215a (see FIG. 8E).
  • the light receiving unit 320 that is, the light emission measuring device performs a second operation to detect the amount of light emitted from the filter unit 220 or its intensity.
  • the amount of light emitted or its intensity may be proportional to the concentration of the microorganism. That is, when the amount of light emission or its intensity is large, the concentration of the microorganism is recognized as large in proportion thereto, and when the amount of light emission or its intensity is small, the concentration of the microorganism may be recognized as proportionally small.
  • the amount of emitted light detected by the second operation of the light receiving unit 320 is the amount of emitted light that can be detected by the filter unit 220 after the collection of the microbial particles, and the amount of emitted light reflecting the concentration of the microbial particles (second emission amount) It is understood as (S22).
  • the controller 450 may determine a light emission amount (microbial light emission amount) corresponding to the concentration of microorganisms collected by the filter unit 220 as a value obtained by subtracting the first light emission amount from the second light emission amount.
  • the controller 450 may display information on the concentration of microorganisms on the display unit 420 based on the amount of light emitted from the microorganisms. For example, depending on the concentration of the microorganism, the lighting unit of different colors may be activated in the display unit 420 (S23).
  • the collection and emission measurement steps of the microbial particles may be automatically and continuously performed, and thus the measurement process of the suspended microorganisms may be easily performed.
  • the user since information on the concentration of the microorganisms may be displayed on the display unit, the user may easily check the concentration of the floating microorganisms.
  • home appliances for air purification may be provided.
  • the home appliance may be driven when the concentration of the floating microorganisms displayed on the display unit is high, that is, when the pollution degree of the floating microorganisms is severe.
  • the household electrical appliance may include an air purifier, a ventilator or an air conditioner. That is, the airborne microbial measurement apparatus may transmit information about the concentration of microorganisms to the home appliances, to guide the operation of the home appliances.
  • the filter part can be sterilized before the microorganism particles are collected in the filter part, it is possible to prevent the microbial particle concentration from being incorrectly calculated by the contaminants in the filter part.
  • the concentration of the microbial particles may be accurately measured.
  • the filter unit in which the classified microorganism particles are collected can be sterilized, contamination of the filter unit can be prevented, and thus, in measuring the concentration of the microbial particles collected in the filter unit, Industrial applicability is remarkable as the effects of contaminants present can be reduced.

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Abstract

La présente invention concerne un appareil de mesure de micro-organisme aérien et son procédé de mesure. L'appareil de mesure de micro-organisme aérien, selon un mode de réalisation de la présente invention, comprend : un appareil de tri de particule ayant une partie entrée pour introduire de l'air, et une partie buse disposée sur un premier côté de la partie entrée ; un trajet d'écoulement de particule de micro-organisme, à travers lequel des particules de micro-organisme, qui passent à travers un trajet d'écoulement interne de la partie buse dans l'air, se déplacent ; un appareil d'entraînement pour amener les particules de micro-organisme à se déplacer ; un appareil de collecte qui est relié au trajet d'écoulement de particule de micro-organisme et qui a une partie filtre, dans laquelle les particules de micro-organisme sont collectées ; un appareil de mesure d'émission de lumière pour détecter la quantité ou l'intensité de lumière générée à partir des particules de micro-organisme collectées dans la partie filtre ; et un appareil de stérilisation disposé sur un premier côté de la partie filtre pour stériliser la partie filtre.
PCT/KR2015/006908 2014-07-28 2015-07-06 Appareil de mesure de micro-organisme aérien et son procédé de mesure WO2016017950A1 (fr)

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KR102421489B1 (ko) * 2020-03-20 2022-07-15 주식회사 파이퀀트 유해성분 측정 장치 및 이를 이용한 유해성분 분석 시스템
KR102514890B1 (ko) 2020-11-24 2023-03-29 한국과학기술연구원 바이오 에어로졸 검출장치 및 검출방법
KR20230102642A (ko) 2021-12-30 2023-07-07 경희대학교 산학협력단 실내공기 중 부유 및 표면 미생물 군집분석을 위한 시료채취방법 및 분석방법

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CN111855365A (zh) * 2020-06-19 2020-10-30 东洋工业(广东)有限公司 一种微生物检测装置
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CN106662576B (zh) 2019-08-02
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KR102221557B1 (ko) 2021-03-02

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