WO2016006603A1 - Lactose-containing coffee beverage - Google Patents

Lactose-containing coffee beverage Download PDF

Info

Publication number
WO2016006603A1
WO2016006603A1 PCT/JP2015/069517 JP2015069517W WO2016006603A1 WO 2016006603 A1 WO2016006603 A1 WO 2016006603A1 JP 2015069517 W JP2015069517 W JP 2015069517W WO 2016006603 A1 WO2016006603 A1 WO 2016006603A1
Authority
WO
WIPO (PCT)
Prior art keywords
coffee
milk
beverage
lactose
glucose
Prior art date
Application number
PCT/JP2015/069517
Other languages
French (fr)
Japanese (ja)
Inventor
鷹明 谷
良介 杉野
佑騎 冨安
Original Assignee
サントリー食品インターナショナル株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by サントリー食品インターナショナル株式会社 filed Critical サントリー食品インターナショナル株式会社
Publication of WO2016006603A1 publication Critical patent/WO2016006603A1/en

Links

Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/152Milk preparations; Milk powder or milk powder preparations containing additives
    • A23C9/156Flavoured milk preparations ; Addition of fruits, vegetables, sugars, sugar alcohols or sweeteners
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F5/00Coffee; Coffee substitutes; Preparations thereof
    • A23F5/10Treating roasted coffee; Preparations produced thereby
    • A23F5/14Treating roasted coffee; Preparations produced thereby using additives, e.g. milk, sugar; Coating, e.g. for preserving

Definitions

  • the present invention relates to a lactose-containing coffee drink obtained by heat sterilization treatment.
  • Coffee drinks containing milk are highly drinkable drinks that are drunk throughout the year, and a large number of coffee drinks containing container-packed milk that can be stored at room temperature for a long period are in circulation.
  • Coffee beverages containing packaged milk are usually made from coffee beans such as coffee bean extract, instant coffee, etc. (also referred to herein as coffee), milk, concentrated milk, whole milk or whole milk powder, skim milk. Or before mixing the raw material containing milk components such as skim milk powder, condensed milk, cream, or milk protein (in this specification, also expressed as milk), etc. Or after being filled, it is manufactured by high temperature sterilization.
  • Lactose contained in milk raw materials has been reported as a causative substance for the taste alteration of beverages containing milk.
  • formic acid is produced and the taste of milk-containing beverages is altered, so that it is proposed to use milk raw materials with reduced lactose in the production of milk-containing beverages (Patent Document 1).
  • a milk that does not deteriorate in flavor over a long period of time even in a heated state characterized by adding a mixture of saccharides and amino acids and a milk component to a coffee extract containing sugar, filling the container and then sterilizing by retort A coffee coffee containing is proposed (Patent Document 2).
  • sucrose having a low temperature dependency of sweetness is used as a saccharide to be blended in a coffee beverage (Non-Patent Document 1).
  • Glucose usually lowers its pH during retort heat sterilization and high-temperature storage. Is said not to be used for coffee beverages manufactured through heat sterilization treatment such as canned coffee (Non-patent Document 2).
  • a coffee beverage in which a part of sucrose is replaced with a reducing monosaccharide such as glucose and subjected to retort sterilization is also disclosed (Patent Document 3).
  • Lactose is an important factor for determining the milk flavor of milk-containing beverages, and is an essential component particularly in the production of milk-containing coffee beverages with a good balance between milk flavor and coffee flavor.
  • An object of the present invention is to provide a coffee drink containing lactose (hereinafter referred to as “lactose-containing coffee drink”) having high drinkability and suppressing deterioration of flavor due to high-temperature sterilization and / or storage in a heated state. It is to provide.
  • glucose is said to be unsuitable for retort heat sterilization in coffee beverages having a milk solid content of less than 3.0% by weight.
  • a coffee beverage obtained by high-temperature sterilization of a liquid mixture obtained by blending lactose and glucose with coffee (I)
  • the lactose content (a) in the beverage is 0.2 to 0.9 g / 100 g
  • the concentration ratio [(b) / (a)] of the lactose content (a) and the glucose content (b) in the beverage is 0.2 to 15, (Iii)
  • the beverage according to (1) which is a coffee beverage containing milk.
  • the beverage according to (1) or (2), wherein the concentration ratio [(b) / (a)] of the lactose content (a) and the glucose content (b) in the beverage is 1.7 to 12.
  • a lactose (preferably with milk) coffee beverage that can withstand quality in terms of flavor from high-temperature sterilization, long-term storage and heating with a product warmer in winter can be obtained.
  • the lactose-containing coffee beverage of the present invention is a beverage with high drinkability in which the off-flavor (milk heating odor and oxidation odor) of the beverage containing milk is reduced and the coffee flavor is enhanced.
  • [Coffee drink] refers to a beverage product produced using a coffee component as a raw material and subjected to a heat sterilization step, and in an amount of 100 g, preferably 1 g or more in terms of green coffee beans (preferably 2.5 g or more, and more preferably 5 g or more) includes coffee components extracted or eluted from coffee beans.
  • the upper limit of the coffee content is not limited, but is 10 g or less, preferably about 9 g or less, in terms of green coffee beans, from the viewpoint of flavor.
  • a beverage blended so that the coffee solid content is 1.0 to 2.0, preferably 1.1 to 1.8 in the beverage is an example of a preferred embodiment of the present invention.
  • the coffee solid content represents a soluble solid content derived from coffee beans, and among the soluble solid content that can be included in the coffee beverage, sweet components such as sugars, milk, pH adjusters, flavoring coffee beans
  • sweet components such as sugars, milk, pH adjusters, flavoring coffee beans
  • the solid content excluding components that are not derived, and the solid content of coffee in the coffee extract is a value determined by measuring Brix (%) using a saccharimeter.
  • the coffee component in the present invention refers to a solution containing a component derived from coffee beans, for example, a coffee extract, that is, a solution obtained by extracting roasted and pulverized coffee beans using water or hot water. Can be mentioned. Moreover, the solution which adjusted the coffee extract which concentrated the coffee extract, the instant coffee which dried the coffee extract, etc. to water and warm water etc. to an appropriate quantity is also mentioned as a coffee part. In particular, from the standpoint that the flavor of the coffee of the present invention is remarkably exhibited, it is preferable to use a coffee extract obtained from roasted coffee beans having an L value of 13 to 35 as a coffee component. In the present specification, unless otherwise specified, the numerical value range includes its end points.
  • the L value is a numerical value of the surface color of the coffee granules obtained by pulverizing roasted coffee beans, and is a value serving as a lightness index (0 is black, 100 is white).
  • the L value of the coffee granules can be measured using, for example, a color difference meter.
  • the coffee beverage of the present invention has an effect that the off-flavor of a beverage containing milk due to lactose is reduced, the richness of milk is imparted, and the coffee flavor is enhanced. More specifically, it is said that the scent felt by human beings can be divided into two categories: orthonasal aroma (also called nasal tip and top scent) and retro nasal aroma (also called mouth and last scent).
  • the coffee beverage of the present invention is a beverage in which the aroma of orthonasal aroma coffee is enhanced and the milk flavor and coffee flavor of retronasal aroma are enhanced.
  • orthonasal aroma refers to the scent that enters the nasal cavity through the inhalation of the nose
  • retro nasal aroma refers to the scent that passes from the throat to the nose when food is placed in the mouth.
  • This preferred retronasal aroma flavor may be inhibited by the presence of vinyl catechol oligomers. This is because when there are many vinyl catechol oligomers, the bitterness is too strong and has a large persistence. As coffee beans are deeply roasted, chlorogenic lactones decrease, and instead vinyl catechol oligomers increase. Therefore, in the coffee beverage of the present invention, a roasted coffee having an L value of 17 to 26, preferably 18 to 25 is used. It is particularly preferable to use a coffee extract obtained from roasted coffee beans as the coffee component.
  • the bitter taste of chlorogenic acids and / or chlorogenic acid lactones can be mentioned.
  • Chlorogenic acid's bitterness and astringent acidity combined with heat-denatured lactose (eg, formic acid), may adversely affect the flavor of beverages containing milk, but the lactose off-flavor of the present invention
  • moderate bitterness of chlorogenic acids is perceived as a preferred coffee flavor.
  • a coffee beverage containing 30 to 140 mg / 100 g of chlorogenic acids, preferably 35 to 120 mg / 100 g, more preferably 40 to 100 mg / 100 g is a preferred embodiment of the present invention.
  • monocaffeoylquinic acid component (3-caffeoylquinic acid, 4-caffeoylquinic acid, 5-caffeoylquinic acid), ferlaquina Acid component (3-ferlaquinic acid, 4-ferlaquinic acid, 5-ferlaquinic acid) and dicaffeoylquinic acid component (3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5- It means the sum of three kinds of dicaffeoylquinic acid).
  • a person skilled in the art can appropriately measure the content of chlorogenic acids in the coffee beverage using, for example, HPLC.
  • the aroma of Orthonasal aroma includes 2-furfuryl methyl disulfide, methyl (2-methyl-3-furyl) disulfide, 2-furfuryl methyl sulfide and methyl (5-methyl-2-furyl) disulfide.
  • One or more aromatic components selected from the group consisting of 2-furfurylmethyl disulfide, methyl (2-methyl-3-furyl) disulfide, 2-furfurylmethyl sulfide and methyl (5-methyl-2-furyl) disulfide;
  • the coffee drink to be included is a preferred embodiment in which the effects of the present invention are more manifested.
  • lactose-containing coffee drink refers to a coffee beverage obtained by blending lactose or milk containing lactose with the above coffee.
  • the lactose content (a) in the beverage is about 0.2 to 0.9 g / 100 g, preferably about 0.3 to 0.8 g / 100 g.
  • lactose less than 0.2 g / 100 g the presence of off-flavor is not very problematic.
  • lactose exceeds 0.9 g / 100g, even if it has the means of this invention, an improvement effect may not be acquired.
  • the coffee beverage of the present invention is one in which the change in flavor associated with the thermal decomposition of lactose is suppressed, and exhibits the maximum effect when it is made into a coffee beverage containing milk.
  • a coffee drink with milk is a preferred embodiment of the present invention.
  • a coffee drink with milk refers to a coffee drink obtained by blending milk into the above coffee.
  • milk component refers to a component added to give a beverage a milk flavor or a milky feeling, and mainly refers to milk, milk and dairy products defined in the Ministerial Ordinance such as milk. For example, raw milk, cow milk, special milk, skim milk, processed milk, milk beverages, etc.
  • dairy products cream, concentrated whey, concentrated milk, skim concentrated milk, sugar-free condensed milk, sweetened skim condensed milk, whole powdered milk, Non-fat dry milk, cream powder, whey powder, butter milk powder, adjusted milk powder and the like can be mentioned.
  • the heat-treated milk such as heat-treated concentrated milk, heat-treated defatted concentrated milk, condensed milk obtained from these, and other powdered milk (whole powdered milk, skimmed milk powder, cream powder) , Whey powder, buttermilk powder, adjusted powdered milk, decalcified powdered milk, etc.
  • a heated odor called cooked flavor on these materials themselves often have a heated odor called cooked flavor on these materials themselves.
  • the cooked flavor of the milk itself is expected to worsen the flavor of the coffee beverage additively or synergistically with the off-flavor caused by the decomposition of lactose by retort heating.
  • coffee beverages that contain glucose at a specific ratio also reduce cooked flavors derived from raw materials. Therefore, in the coffee beverage of the present invention, heat-treated milk can also be suitably used.
  • the milk content in the milk-containing beverage of the present invention is such that the milk solid content is less than 3.0 wt% from the viewpoint of obtaining the beverage stability and the remarkable effects of the present invention.
  • milk solid content means the total amount of non-fat milk solid content and milk fat content, and after drying the milk using a general drying method (freeze drying, evaporation to dryness, etc.) to remove water It means the dried product.
  • it is preferable to set it as 0.2 weight% or more and less than 3.0 weight% in conversion of non-fat milk solid content, It is more preferable to set it as 0.4 weight% or more and less than 3.0 weight%, 0.8 weight% % Or more and less than 3.0% by weight is more preferable.
  • the calcium content in the coffee beverage of the present invention is 26 mg / 100 g or less, preferably 20 mg / 100 g or less, more preferably 16 mg / 100 g or less, still more preferably 15 mg / 100 g or less, particularly preferably 14 mg / 100 g or less.
  • the calcium content in the beverage can be achieved by using milk that has been processed to reduce calcium.
  • the method for decalcification is not particularly limited, and a known method such as a precipitation method by maintaining the temperature can be employed.
  • a coffee beverage containing milk fat is one of the preferred embodiments of the present invention.
  • the milk solid content in the beverage as milk is less than 3.0% by weight even when milk fat is contained.
  • the lactose-containing beverage of the present invention is characterized by blending a specific amount of glucose.
  • the concentration ratio [(b) / (a)] of the glucose content (b) in the beverage and the lactose content (a) in the beverage is 0.2 or more, preferably 0.5 or more, more preferably 1 0.0 or more, more preferably 1.5 or more, and particularly preferably 1.7 or more.
  • the upper limit of (b) / (a) is 15 or less, preferably 12 or less, more preferably 10 or less, still more preferably 7.5 or less, and particularly preferably 6 or less.
  • the present invention by adding glucose, which is known as a heat-denatured component, at a certain ratio in order to suppress the flavor denaturation due to heat, in a coffee beverage containing milk, the flavor is obtained by high-temperature sterilization and / or storage in a warmed state.
  • glucose content (b) is too high, a change in flavor due to a decrease in pH due to thermal denaturation of glucose itself increases, impairing the effects of the present invention.
  • the glucose content is preferably 0.1 to 10%, preferably 0.5 to 7.0% in the beverage.
  • the coffee beverage of the present invention contains lactose and glucose as essential components, but other saccharides (sucrose, isomerized sugar, fructose, maltose, oligosaccharide, etc.) may be added as desired.
  • saccharides saccharides
  • fructose, maltose may inhibit the effect of the glucose of the present invention
  • the glucose content in the beverage (b) is 0.8 or less, preferably The amount is preferably 0.5 or less.
  • the effects of the present invention are remarkably exhibited when the sugar concentration in the beverage is in the range of 1.0 to 10.0 g / 100 g.
  • the saccharide concentration in the beverage refers to the total amount of glucose, lactose, sucrose, fructose and maltose present in the beverage.
  • packed beverage refers to a single strength, that is, a beverage that can be consumed as it is without being diluted as a normal after opening the packaged beverage. Is clearly excluded from the category.
  • the coffee beverage of the present invention is a container-packed coffee beverage manufactured by heat sterilization.
  • Canned coffee beverages are made by blending milk into coffee and then homogenizing a mixture of glucose and other ingredients, if necessary, and filling into metal cans, followed by retort sterilization under the sterilization conditions stipulated in the Food Sanitation Law. It can be processed and manufactured. That is, the canned coffee beverage of the present invention (preferably 2-furfurylmethyl disulfide, methyl (2-methyl-3-furyl) disulfide, 2-furfurylmethyl sulfide and methyl (5-methyl-2-furyl) disulfide) Canned coffee beverages containing one or more fragrance components selected from the group consisting of: are produced through the following steps 1 to 3. Step 1 of blending lactose and glucose with coffee to obtain a mixed solution, Step 2 of filling the preparation liquid into a container, and Step 3 of retort sterilization treatment
  • the ratio (weight ratio) of glucose (b) to lactose (a) is 0.2 or more, preferably 0.5 or more, more preferably 1.0 or more, and still more preferably 1. It mix
  • the upper limit is 15 or less, preferably 12 or less, more preferably 10 or less, still more preferably 7.5 or less, and particularly preferably 6 or less, the lactose and glucose in the beverage after the high temperature sterilization treatment. The content ratio tends to fall within this range.
  • the effect of the present invention of suppressing the alteration of flavor due to high temperature sterilization and / or storage in a warmed state can be expressed more strongly. it can.
  • the caffeine content in the preferred preparation is about 30 to 95 mg / 100 g, preferably about 45 to 85 mg / 100 g.
  • the caffeine content in the preparation liquid can be determined by measuring the caffeine content derived from the coffee component and, if insufficient, caffeine or the like designated by the food additive can be appropriately added and used. A person skilled in the art can appropriately measure the content of caffeine in a coffee beverage using, for example, HPLC.
  • the preparation liquid in step 1 is adjusted to about pH 5.5 to 8.0 with a pH adjuster.
  • a pH adjuster When pH is low, the aftertaste bitterness and miscellaneous taste by the heating odor and miscellaneous taste accompanying the retort sterilization of process 3 will become remarkable, and the effect of this invention may be inhibited.
  • the pH of the preparation is adjusted so that the content after heat sterilization has a pH of 5.5 to 7.0, preferably about 6.0 to 7.0.
  • the pH adjuster include sodium carbonate, sodium hydrogen carbonate, disodium hydrogen phosphate, potassium carbonate, and the like. From the viewpoint of the effect of the present invention, an alkaline sodium salt or an alkaline potassium salt is preferable, and sodium hydrogen carbonate is most preferable.
  • the coffee flavor inherent in the coffee component is enhanced, and the coffee beverage has a balanced milk flavor and coffee flavor.
  • ingredients can be blended in the coffee beverage of the present invention as desired.
  • Other raw materials include stabilizers such as sodium caseinate, antioxidants, emulsifiers, and fragrances.
  • the caffeine concentration in the beverage was quantified by diluting the coffee beverage 10-fold with mobile phase A (w / w), filtering with a membrane filter (Cellulose Acetate 0.45 ⁇ m manufactured by ADVANTEC), and injecting it into HPLC.
  • the HPLC measurement conditions are as follows.
  • the chlorogenic acid concentration in the beverage was quantified by diluting the coffee beverage 10-fold with mobile phase A (w / w), filtering with a membrane filter (Cellulose Acetate 0.45 ⁇ m manufactured by ADVANTEC), and injecting it into HPLC.
  • the HPLC measurement conditions are as follows.
  • Example 1 As coffee, an existing coffee extract produced from Colombian coffee beans was used. The coffee extract was blended with 113 mL / L of milk, diluted with water so that the coffee solid content was 1.55%, filtered through 500 mesh to remove insoluble solid content, and used (hereinafter referred to as coffee blending solution). Called).
  • the obtained container-packed coffee beverage (milk solid content: 1.49 g / 100 g, sugar concentration: 6.0 g / 100 g, pH 6.3) was subjected to sensory evaluation by a specialized panel. Evaluation is No. 1 as the control (3 points) for the strength of the fragrant fragrance (top fragrance) at the top and the intensity of the fragrant fragrance (last fragrance) felt in the aftertaste. ) 4 points: stronger than control (preferred), 3 points: comparable to control, 2 points: weaker than control (not preferable), 1 point: very weaker than control (not preferable) The average score was calculated from the results. In addition, regarding the strength of milk deterioration odor, No.
  • Example 2 The milk was centrifuged and separated into fatty cream and lean skimmed milk. After passing this skimmed milk through a tube filled with an ion exchange resin to remove calcium ions, this was mixed with the cream obtained by centrifuging, and this liquid was dried to obtain decalcified powdered milk. .
  • Example 1 No. of Example 1 except that the milk content in Example 1 is changed to the combined use of milk and decalcified milk powder in the proportions shown in Table 2.
  • a canned retort sterilized coffee beverage was produced in the same manner as the beverage of No. 5.
  • the obtained canned coffee beverage (milk solid content: 1.49 g / 100 g, sugar concentration: 6.0 g / 100 g, pH 6.3) was evaluated in the same manner as in Example 1. The results are shown in Table 3. In both cases, the coffee flavor was enhanced, and it was a drinkable milk coffee beverage with a good balance between milk flavor and coffee flavor. It was suggested that as the calcium concentration in the beverage decreases, the milk deterioration odor can be further reduced, and the coffee aroma is enhanced.
  • Example 3 Brazilian coffee beans (L value of about 20) are pulverized with a pulverizer (manufactured by Nippon Granulator Co., Ltd.), drip with 94 ° C hot water to obtain a coffee extract of Brix 2.9, filtered through 500 mesh and insoluble Solids were removed.
  • a pulverizer manufactured by Nippon Granulator Co., Ltd.
  • glucose and sucrose are blended in various proportions so that the total amount is 59.0-74.0 g / L (amount in anticipation of decomposition by heating), and milk (55 mL of milk) is added.
  • Example Nos. 11 to 15 a canned coffee drink was prepared in the same manner except that only sucrose was used without adding glucose (sample No. 10).
  • the obtained canned coffee beverage (chlorogenic acids: 63 mg / 100 g, milk solid content: 0.72 g / 100 g, sugar concentration: 6.0 g / 100 g, pH 6.3) was compared with the control in the same manner as in Example 1. Sensory evaluation was performed. The results are shown in Table 4.
  • Example 4 In the coffee extract of Example 3, glucose and sucrose are blended in various proportions so that the total amount is 56.0 to 70.0 g / L (amount in anticipation of decomposition by heating), and milk (135 mL of milk) is further added. / L) A canned coffee drink was produced in the same manner as in Example 3. The obtained canned coffee beverage (chlorogenic acids: 63 mg / 100 g, milk solid content: 1.78 g / 100 g, sugar concentration: 6.0 g / 100 g, pH 6.3) was subjected to sensory evaluation in the same manner as in Example 3. It was. The results are shown in Table 5.
  • Example 5 In the coffee extract of Example 3, glucose and sucrose are blended in various proportions so that the total amount is 52.0 to 65.0 g / L (amount in anticipation of decomposition by heating), and milk (185 mL of milk) is further added. / L) A canned coffee drink was produced in the same manner as in Example 3. The obtained canned coffee beverage (chlorogenic acids: 63 mg / 100 g, milk solid content: 2.81 g / 100 g, sugar concentration: 6.0 g / 100 g, pH 6.3) was subjected to sensory evaluation in the same manner as in Example 3. It was. The results are shown in Table 6.
  • Example 6 A canned coffee beverage is produced in the same manner as in Example 3 except that 40 g / L of glucose and 63 g / L of sucrose are added to the coffee extract of Example 3 and the milk content (milk 135 mL / L) is further added. (Sample No. 29). Moreover, the canned coffee drink which mix
  • Example 7 A canned coffee beverage was produced in the same manner as in Example 6 except that the amounts of glucose and sucrose were 4 g / L and 7 g / L, and the milk content (milk 130 mL / L) was used (Sample No. 31). Moreover, the canned coffee drink which mix
  • Example 8 No. 5 in Example 5.
  • a canned coffee drink was produced in the same manner as in Example 5 except that the glucose of 23 was 0.19 g / 100 g, and the blending ratio of skim milk powder and the decalcified milk powder prepared in Example 2 was changed as milk.
  • the resulting canned coffee beverage (chlorogenic acids: 63 mg / 100 g, milk solids: 2.05 to 2.81 g / 100 g, sugar concentration: 5.9 g / 100 g, pH 6.3) does not contain glucose Sensory evaluation was performed in comparison with the control (sample No. 22).
  • Coffee beverages with a concentration ratio [(b) / (a)] of lactose content (a) in the beverage of 0.2 or more show improvement in any of the evaluation items of top fragrance, last fragrance, and milk odor.
  • concentration ratio [(b) / (a)] of lactose content (a) in the beverage of 0.2 or more show improvement in any of the evaluation items of top fragrance, last fragrance, and milk odor.
  • the glucose and lactose content is the same, that is, when (b) / (a) is the same, the lower the calcium concentration, the lower the milk deterioration odor.
  • the scent of was enhanced.
  • Example 9 Using a commercially available milk beverage (sugar-free type, sweetener (acesulfame potassium) blended), blended at a concentration of 5.5 g / L glucose and 0.53 g / 100 g (sample No.) 37) was subjected to sensory evaluation using a commercially available milk beverage as a control (sample No. 36). The results are shown in Table 10. In beverages containing glucose, the preferred coffee flavor was enhanced and beverages with higher drinkability were obtained.

Abstract

Provided is a lactose-containing coffee beverage which has superior drinkability and in which changes in flavor due to high-temperature pasteurization and/or storage in a heated state have been suppressed. The beverage is a coffee beverage containing milk obtained by subjecting a liquid preparation obtained by combining lactose and glucose with a coffee component to high-temperature pasteurization, wherein (i) the lactose content (a) of the beverage is 0.2 to 0.9g/100g, (ii) the concentration ratio [(b)/(a)] of the lactose content (a) and the glucose content (b) of the beverage is 0.2 to 15, and (iii) milk solids make up less than 3.0wt% of the beverage.

Description

乳糖を含有するコーヒー飲料Coffee drink containing lactose
 本発明は、加熱殺菌処理して得られる乳糖含有コーヒー飲料に関する。 The present invention relates to a lactose-containing coffee drink obtained by heat sterilization treatment.
 ミルク入りのコーヒー飲料は、一年を通して飲用される嗜好性の高い飲料であり、長期にわたって常温保存可能な容器詰めミルク入りコーヒー飲料が多数流通されている。容器詰めミルク入りコーヒー飲料は、通常、コーヒー豆抽出液、インスタントコーヒー等のコーヒー原料(本明細書中、コーヒー分とも表記する)に、牛乳、濃縮乳、全脂乳又は全脂粉乳、脱脂乳又は脱脂粉乳、練乳、クリーム、或いは乳タンパク質等のミルク成分を含有する乳原料(本明細書中、乳分とも表記する)などを混合溶解して調合液を得、保存容器に充填される前、または充填された後のいずれかに、高温殺菌をして製造されている。このように高温殺菌を経て製造されるミルク入り飲料では、ミルク成分が熱変性し、乳加熱臭、具体的には、乳独特の劣化臭(すえ臭)や乳独特のむれっぽい味を発生させ、コク(クリーミー感)が消失して、ミルク入り飲料の品質を低下させることが知られている。 】 Coffee drinks containing milk are highly drinkable drinks that are drunk throughout the year, and a large number of coffee drinks containing container-packed milk that can be stored at room temperature for a long period are in circulation. Coffee beverages containing packaged milk are usually made from coffee beans such as coffee bean extract, instant coffee, etc. (also referred to herein as coffee), milk, concentrated milk, whole milk or whole milk powder, skim milk. Or before mixing the raw material containing milk components such as skim milk powder, condensed milk, cream, or milk protein (in this specification, also expressed as milk), etc. Or after being filled, it is manufactured by high temperature sterilization. In beverages containing milk manufactured through high-temperature sterilization in this way, the milk components are heat-denatured, producing a heated odor of milk, specifically, a deteriorated odor unique to milk and a peculiar milky taste. It is known that body (creamy feeling) disappears and the quality of the milk-containing beverage is lowered.
 ミルク入り飲料の味変質の原因物質として、乳原料に含まれている乳糖が報告されている。乳糖が熱分解された結果、ギ酸が生じてミルク入り飲料の味変質を起こしていることから、ミルク入り飲料の製造において乳糖を低減した乳原料を用いることが提案されている(特許文献1)。また、糖分を含むコーヒー抽出液に、単糖およびアミノ酸からなる混合物ならびに乳成分を添加し、容器に充填後、レトルト殺菌することを特徴とする、加温状態でも長期間にわたり香味劣化のないミルク入りコーヒー飲料が提案されている(特許文献2)。 Lactose contained in milk raw materials has been reported as a causative substance for the taste alteration of beverages containing milk. As a result of the thermal decomposition of lactose, formic acid is produced and the taste of milk-containing beverages is altered, so that it is proposed to use milk raw materials with reduced lactose in the production of milk-containing beverages (Patent Document 1). . A milk that does not deteriorate in flavor over a long period of time even in a heated state, characterized by adding a mixture of saccharides and amino acids and a milk component to a coffee extract containing sugar, filling the container and then sterilizing by retort A coffee coffee containing is proposed (Patent Document 2).
 一般に、コーヒー飲料に配合される糖類としては、甘味度の温度依存性が小さいショ糖が使われ(非特許文献1)、ブドウ糖はレトルト加熱殺菌・高温保存中にpHの低下をきたすため、通常は缶コーヒーのような加熱殺菌処理を経て製造されるコーヒー飲料には使用しないと言われている(非特許文献2)。しかし、乳成分の分離を抑制することを目的として、ショ糖の一部をブドウ糖のような還元性単糖で置換してレトルト殺菌処理されたコーヒー飲料も開示されている(特許文献3)。 In general, sucrose having a low temperature dependency of sweetness is used as a saccharide to be blended in a coffee beverage (Non-Patent Document 1). Glucose usually lowers its pH during retort heat sterilization and high-temperature storage. Is said not to be used for coffee beverages manufactured through heat sterilization treatment such as canned coffee (Non-patent Document 2). However, for the purpose of suppressing separation of milk components, a coffee beverage in which a part of sucrose is replaced with a reducing monosaccharide such as glucose and subjected to retort sterilization is also disclosed (Patent Document 3).
特開平11-113493号公報Japanese Patent Laid-Open No. 11-113493 特開平11-9190号公報Japanese Patent Laid-Open No. 11-9190 特開平9-205990号公報Japanese Patent Laid-Open No. 9-205990
 乳糖は、ミルク入り飲料の乳風味を決定するのに重要な因子であり、特に乳風味とコーヒー風味のバランスの取れたミルク入りコーヒー飲料の製造においては必須の成分である。 Lactose is an important factor for determining the milk flavor of milk-containing beverages, and is an essential component particularly in the production of milk-containing coffee beverages with a good balance between milk flavor and coffee flavor.
 本発明の目的は、高温殺菌及び/又は加温状態での保存による風味の変質を抑制したドリンカビリティの高い、乳糖を含有するコーヒー飲料(以下、「乳糖含有コーヒー飲料」と表記する)を提供することにある。 An object of the present invention is to provide a coffee drink containing lactose (hereinafter referred to as “lactose-containing coffee drink”) having high drinkability and suppressing deterioration of flavor due to high-temperature sterilization and / or storage in a heated state. It is to provide.
 本発明者らは上記課題を解決するために鋭意検討を行った結果、驚くべきことに、乳固形分が3.0重量%未満のコーヒー飲料において、レトルト加熱殺菌に不向きと言われているブドウ糖を、還元糖であり熱変性しやすい乳糖に対して一定割合で配合させることにより、高温殺菌及び/又は加温状態での保存による風味の変質を抑制できることを見出し、本発明を完成するに至った。すなわち本発明は、以下に関する。
(1)コーヒー分に乳糖及びブドウ糖を配合して得られる調合液を高温殺菌して得られるコーヒー飲料であって、以下、
(i)飲料中の乳糖含量(a)が、0.2~0.9g/100g
(ii)飲料中の乳糖含量(a)とブドウ糖含量(b)の濃度比[(b)/(a)]が、0.2~15、
(iii)乳固形分が3.0重量%未満を満たす前記飲料。
(2)ミルク入りコーヒー飲料である、(1)に記載の飲料。
(3)飲料中の乳糖含量(a)とブドウ糖含量(b)の濃度比[(b)/(a)]が1.7~12である、(1)又は(2)に記載の飲料。
As a result of intensive studies to solve the above problems, the present inventors have surprisingly found that glucose is said to be unsuitable for retort heat sterilization in coffee beverages having a milk solid content of less than 3.0% by weight. Has been found to be able to suppress deterioration in flavor due to high-temperature sterilization and / or storage in a warmed state by adding a certain proportion to lactose, which is a reducing sugar and is easily heat-denatured, leading to the completion of the present invention. It was. That is, the present invention relates to the following.
(1) A coffee beverage obtained by high-temperature sterilization of a liquid mixture obtained by blending lactose and glucose with coffee,
(I) The lactose content (a) in the beverage is 0.2 to 0.9 g / 100 g
(Ii) The concentration ratio [(b) / (a)] of the lactose content (a) and the glucose content (b) in the beverage is 0.2 to 15,
(Iii) The beverage having a milk solid content of less than 3.0% by weight.
(2) The beverage according to (1), which is a coffee beverage containing milk.
(3) The beverage according to (1) or (2), wherein the concentration ratio [(b) / (a)] of the lactose content (a) and the glucose content (b) in the beverage is 1.7 to 12.
 本発明により、高温殺菌、長期間の保存および冬季の製品ウォーマーでの加熱にも香味の観点から品質的に耐えうる、乳糖(好ましくは、ミルク入り)コーヒー飲料が得られる。本発明の乳糖含有コーヒー飲料は、ミルク入り飲料のオフフレーバー(乳加熱臭や酸化臭)が低減され、かつ、コーヒー風味が増強されたドリンカビリティの高い飲料である。 According to the present invention, a lactose (preferably with milk) coffee beverage that can withstand quality in terms of flavor from high-temperature sterilization, long-term storage and heating with a product warmer in winter can be obtained. The lactose-containing coffee beverage of the present invention is a beverage with high drinkability in which the off-flavor (milk heating odor and oxidation odor) of the beverage containing milk is reduced and the coffee flavor is enhanced.
 [コーヒー飲料]
 本明細書でいう「コーヒー飲料」とは、コーヒー分を原料として使用し、加熱殺菌工程を経て製造される飲料製品のことをいい、内容量100g中にコーヒー生豆換算で1g以上(好ましくは2.5g以上、より好ましくは5g以上)のコーヒー豆から抽出又は溶出したコーヒー分を含むものをいう。コーヒー分の上限は制限されないが、香味の観点からコーヒー生豆換算で10g以下、好ましくは9g以下程度である。特に、コーヒー固形分が飲料中に1.0~2.0、好ましくは1.1~1.8となるように配合された飲料は、本発明の好ましい態様の一例である。ここで、コーヒー固形分とは、コーヒー豆由来の可溶性固形分を表し、コーヒー飲料に含まれ得る可溶性固形分のうち、糖類などの甘味成分、乳分、pH調整剤、香料等のコーヒー豆に由来しない成分を除いた固形分で、コーヒー抽出液中のコーヒー固形分は糖度計を用いてBrix(%)を測定することにより求められる値である。
[Coffee drink]
The term “coffee beverage” as used herein refers to a beverage product produced using a coffee component as a raw material and subjected to a heat sterilization step, and in an amount of 100 g, preferably 1 g or more in terms of green coffee beans (preferably 2.5 g or more, and more preferably 5 g or more) includes coffee components extracted or eluted from coffee beans. The upper limit of the coffee content is not limited, but is 10 g or less, preferably about 9 g or less, in terms of green coffee beans, from the viewpoint of flavor. In particular, a beverage blended so that the coffee solid content is 1.0 to 2.0, preferably 1.1 to 1.8 in the beverage is an example of a preferred embodiment of the present invention. Here, the coffee solid content represents a soluble solid content derived from coffee beans, and among the soluble solid content that can be included in the coffee beverage, sweet components such as sugars, milk, pH adjusters, flavoring coffee beans The solid content excluding components that are not derived, and the solid content of coffee in the coffee extract is a value determined by measuring Brix (%) using a saccharimeter.
 本発明におけるコーヒー分とは、コーヒー豆由来の成分を含有する溶液のことをいい、例えば、コーヒー抽出液、すなわち、焙煎、粉砕されたコーヒー豆を水や温水などを用いて抽出した溶液が挙げられる。また、コーヒー抽出液を濃縮したコーヒーエキス、コーヒー抽出液を乾燥したインスタントコーヒーなどを、水や温水などで適量に調整した溶液も、コーヒー分として挙げられる。特に、本発明のコーヒーの風味が顕著に発揮される観点から、L値13~35の焙煎コーヒー豆より得られたコーヒー抽出液をコーヒー分として用いることが好ましい。なお、本明細書中、断りがない限り数値範囲はその端点を含むものとする。ここで、L値とは、焙煎コーヒー豆を粉砕したコーヒー顆粒の表面色を数値化したもので、明度の指標となる値である(0が黒、100が白)。コーヒー顆粒のL値は、例えば色彩色差計を用いて測定することができる。 The coffee component in the present invention refers to a solution containing a component derived from coffee beans, for example, a coffee extract, that is, a solution obtained by extracting roasted and pulverized coffee beans using water or hot water. Can be mentioned. Moreover, the solution which adjusted the coffee extract which concentrated the coffee extract, the instant coffee which dried the coffee extract, etc. to water and warm water etc. to an appropriate quantity is also mentioned as a coffee part. In particular, from the standpoint that the flavor of the coffee of the present invention is remarkably exhibited, it is preferable to use a coffee extract obtained from roasted coffee beans having an L value of 13 to 35 as a coffee component. In the present specification, unless otherwise specified, the numerical value range includes its end points. Here, the L value is a numerical value of the surface color of the coffee granules obtained by pulverizing roasted coffee beans, and is a value serving as a lightness index (0 is black, 100 is white). The L value of the coffee granules can be measured using, for example, a color difference meter.
 本発明のコーヒー飲料は、乳糖に起因するミルク入り飲料のオフフレーバーが低減され、ミルクのコクが付与されるとともに、コーヒー風味が増強されるという効果を有する。より詳述すると、人間が感じる香りは、オルソネーザルアロマ(鼻先香、トップ香ともいう)とレトロネーザルアロマ(口中香、ラスト香ともいう)の2つに分けられると言われているが、本発明のコーヒー飲料は、オルソネーザルアロマのコーヒーの香気が増強され、レトロネーザルアロマのミルク風味及びコーヒー風味が増強された飲料となる。ここで、オルソネーザルアロマとは鼻先から鼻腔内に吸気に乗って入ってくる香りをいい、レトロネーザルアロマとは食べ物を口に入れた際に喉から鼻に抜ける香りをいう。 The coffee beverage of the present invention has an effect that the off-flavor of a beverage containing milk due to lactose is reduced, the richness of milk is imparted, and the coffee flavor is enhanced. More specifically, it is said that the scent felt by human beings can be divided into two categories: orthonasal aroma (also called nasal tip and top scent) and retro nasal aroma (also called mouth and last scent). The coffee beverage of the present invention is a beverage in which the aroma of orthonasal aroma coffee is enhanced and the milk flavor and coffee flavor of retronasal aroma are enhanced. Here, orthonasal aroma refers to the scent that enters the nasal cavity through the inhalation of the nose, and retro nasal aroma refers to the scent that passes from the throat to the nose when food is placed in the mouth.
 この好ましいレトロネーザルアロマの風味は、ビニルカテコールオリゴマーの存在により阻害されることがある。ビニルカテコールオリゴマーが多いと、強すぎる、残留性の大きい苦味となるためである。コーヒー豆は、深煎りにしていくにしたがってクロロゲン酸ラクトン類が減少し、代わりにビニルカテコールオリゴマーが増加することから、本発明のコーヒー飲料では、L値17~26、好ましくは18~25の焙煎コーヒー豆より得られたコーヒー抽出液をコーヒー分として用いることが特に好ましい。 This preferred retronasal aroma flavor may be inhibited by the presence of vinyl catechol oligomers. This is because when there are many vinyl catechol oligomers, the bitterness is too strong and has a large persistence. As coffee beans are deeply roasted, chlorogenic lactones decrease, and instead vinyl catechol oligomers increase. Therefore, in the coffee beverage of the present invention, a roasted coffee having an L value of 17 to 26, preferably 18 to 25 is used. It is particularly preferable to use a coffee extract obtained from roasted coffee beans as the coffee component.
 レトロネーザルアロマのコーヒー風味の一つとして、クロロゲン酸類及び/又はクロロゲン酸ラクトン類の苦味が挙げられる。クロロゲン酸の苦味や収斂性のともなった酸味は、熱変性された乳糖(例えばギ酸)とあいまってミルク入り飲料の風味を相加的に悪くすることがあるが、本発明の乳糖のオフフレーバーが低減されたコーヒー飲料では、クロロゲン酸類の適度な苦味が、好ましいコーヒー風味として知覚される。クロロゲン酸類を30~140mg/100g、好ましくは35~120mg/100g、より好ましくは40~100mg/100g含有するコーヒー飲料は、本発明の好適な態様である。ここで本明細書中、コーヒー飲料中のクロロゲン酸類の含量をいうときは、モノカフェオイルキナ酸成分(3-カフェオイルキナ酸、4-カフェオイルキナ酸、5-カフェオイルキナ酸)、フェルラキナ酸成分(3-フェルラキナ酸、4-フェルラキナ酸、5-フェルラキナ酸)及びジカフェオイルキナ酸成分(3,4-ジカフェオイルキナ酸、3,5-ジカフェオイルキナ酸、4,5-ジカフェオイルキナ酸)の三種の合計を意味する。コーヒー飲料中のクロロゲン酸類の含量は、当業者であれば、例えばHPLCを用いて、適宜測定することができる。 As one of the coffee flavors of Retro Nasal Aroma, the bitter taste of chlorogenic acids and / or chlorogenic acid lactones can be mentioned. Chlorogenic acid's bitterness and astringent acidity, combined with heat-denatured lactose (eg, formic acid), may adversely affect the flavor of beverages containing milk, but the lactose off-flavor of the present invention In reduced coffee beverages, moderate bitterness of chlorogenic acids is perceived as a preferred coffee flavor. A coffee beverage containing 30 to 140 mg / 100 g of chlorogenic acids, preferably 35 to 120 mg / 100 g, more preferably 40 to 100 mg / 100 g is a preferred embodiment of the present invention. Here, in this specification, when referring to the content of chlorogenic acids in a coffee beverage, monocaffeoylquinic acid component (3-caffeoylquinic acid, 4-caffeoylquinic acid, 5-caffeoylquinic acid), ferlaquina Acid component (3-ferlaquinic acid, 4-ferlaquinic acid, 5-ferlaquinic acid) and dicaffeoylquinic acid component (3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5- It means the sum of three kinds of dicaffeoylquinic acid). A person skilled in the art can appropriately measure the content of chlorogenic acids in the coffee beverage using, for example, HPLC.
 また、オルソネーザルアロマのコーヒーの香りとしては、2-フルフリルメチルジスルフィド、メチル(2-メチル-3-フリル)ジスルフィド、2-フルフリルメチルスルフィドおよびメチル(5-メチル-2-フリル)ジスルフィドを挙げることができる。2-フルフリルメチルジスルフィド、メチル(2-メチル-3-フリル)ジスルフィド、2-フルフリルメチルスルフィドおよびメチル(5-メチル-2-フリル)ジスルフィドからなる群から選択される一以上の香気成分を含むコーヒー飲料は、本発明の効果がより発現される好ましい態様である。 In addition, the aroma of Orthonasal aroma includes 2-furfuryl methyl disulfide, methyl (2-methyl-3-furyl) disulfide, 2-furfuryl methyl sulfide and methyl (5-methyl-2-furyl) disulfide. Can be mentioned. One or more aromatic components selected from the group consisting of 2-furfurylmethyl disulfide, methyl (2-methyl-3-furyl) disulfide, 2-furfurylmethyl sulfide and methyl (5-methyl-2-furyl) disulfide; The coffee drink to be included is a preferred embodiment in which the effects of the present invention are more manifested.
 [乳糖含有コーヒー飲料]
 本発明の「乳糖含有コーヒー飲料」とは、上記コーヒー分に乳糖又は乳糖を含有する乳分を配合して得られるコーヒー飲料をいう。飲料中の乳糖含有量(a)は、0.2~0.9g/100g、好ましくは0.3~0.8g/100g程度である。乳糖が0.2g/100g未満の飲料ではオフフレーバーの存在があまり問題視されない。また、乳糖が0.9g/100gを超える飲料では、本発明の手段をもってしても改善効果が得られないことがある。
[Lactose-containing coffee drink]
The “lactose-containing coffee beverage” of the present invention refers to a coffee beverage obtained by blending lactose or milk containing lactose with the above coffee. The lactose content (a) in the beverage is about 0.2 to 0.9 g / 100 g, preferably about 0.3 to 0.8 g / 100 g. In beverages with lactose less than 0.2 g / 100 g, the presence of off-flavor is not very problematic. Moreover, in the drink in which lactose exceeds 0.9 g / 100g, even if it has the means of this invention, an improvement effect may not be acquired.
 本発明のコーヒー飲料は、乳糖の熱分解に伴う風味変化が抑制されたもので、ミルク入りコーヒー飲料とした場合に、最大限の効果を発揮する。ミルク入りコーヒー飲料は、本発明の好ましい態様である。本明細書でいう「ミルク入りコーヒー飲料」とは、上記コーヒー分に乳分を配合して得られるコーヒー飲料をいう。ここで、乳分とは、飲料にミルク風味やミルク感を付与するために添加する成分を指し、主に乳等省令に定義されている乳、牛乳及び乳製品のことをいう。例えば、生乳、牛乳、特別牛乳、脱脂乳、加工乳、乳飲料等が挙げられ、乳製品としては、クリーム、濃縮ホエイ、濃縮乳、脱脂濃縮乳、無糖練乳、加糖脱脂練乳、全粉乳、脱脂粉乳、クリームパウダー、ホエイパウダー、バターミルクパウダー、調整粉乳などが挙げられる。これら牛乳及び乳製品のうち、加熱処理が行われている乳分、例えば加熱処理濃縮乳、加熱処理脱脂濃縮乳やこれらを原料として得られる練乳、その他粉乳類(全粉乳、脱脂粉乳、クリームパウダー、ホエイパウダー、バターミルクパウダー、調整粉乳、脱カルシウム粉乳など)は、これら素材自体にクックドフレーバーと呼ばれる加熱臭を帯びていることが多い。この乳分自体の有するクックドフレーバーは、乳糖のレトルト加熱による分解に起因するオフフレーバーと相加的又は相乗的にコーヒー飲料の風味を悪くすることが予想されるが、本発明の乳糖に対してブドウ糖を特定割合で配合したコーヒー飲料は、原料由来のクックドフレーバーも低減される。したがって、本発明のコーヒー飲料では、加熱処理された乳分も好適に用いることができる。 The coffee beverage of the present invention is one in which the change in flavor associated with the thermal decomposition of lactose is suppressed, and exhibits the maximum effect when it is made into a coffee beverage containing milk. A coffee drink with milk is a preferred embodiment of the present invention. As used herein, “a coffee drink with milk” refers to a coffee drink obtained by blending milk into the above coffee. As used herein, the term “milk component” refers to a component added to give a beverage a milk flavor or a milky feeling, and mainly refers to milk, milk and dairy products defined in the Ministerial Ordinance such as milk. For example, raw milk, cow milk, special milk, skim milk, processed milk, milk beverages, etc. are mentioned, and as dairy products, cream, concentrated whey, concentrated milk, skim concentrated milk, sugar-free condensed milk, sweetened skim condensed milk, whole powdered milk, Non-fat dry milk, cream powder, whey powder, butter milk powder, adjusted milk powder and the like can be mentioned. Of these milk and dairy products, the heat-treated milk, such as heat-treated concentrated milk, heat-treated defatted concentrated milk, condensed milk obtained from these, and other powdered milk (whole powdered milk, skimmed milk powder, cream powder) , Whey powder, buttermilk powder, adjusted powdered milk, decalcified powdered milk, etc.) often have a heated odor called cooked flavor on these materials themselves. The cooked flavor of the milk itself is expected to worsen the flavor of the coffee beverage additively or synergistically with the off-flavor caused by the decomposition of lactose by retort heating. In addition, coffee beverages that contain glucose at a specific ratio also reduce cooked flavors derived from raw materials. Therefore, in the coffee beverage of the present invention, heat-treated milk can also be suitably used.
 本発明のミルク入り飲料における乳分の含有量は、飲料の安定性と本発明の顕著な効果が得られる観点とから、乳固形分が3.0重量%未満とする。ここで、乳固形分とは無脂乳固形分と乳脂肪分の総量をいい、乳分を一般的な乾燥法(凍結乾燥、蒸発乾固等)を用いて乾燥させて水分を除いた後の乾固物のことをいう。中でも、無脂乳固形分換算で0.2重量%以上3.0重量%未満とすることが好ましく、0.4重量%以上3.0重量%未満とすることがより好ましく、0.8重量%以上3.0重量%未満とすることがさらに好ましい。 </ RTI> The milk content in the milk-containing beverage of the present invention is such that the milk solid content is less than 3.0 wt% from the viewpoint of obtaining the beverage stability and the remarkable effects of the present invention. Here, milk solid content means the total amount of non-fat milk solid content and milk fat content, and after drying the milk using a general drying method (freeze drying, evaporation to dryness, etc.) to remove water It means the dried product. Especially, it is preferable to set it as 0.2 weight% or more and less than 3.0 weight% in conversion of non-fat milk solid content, It is more preferable to set it as 0.4 weight% or more and less than 3.0 weight%, 0.8 weight% % Or more and less than 3.0% by weight is more preferable.
 ミルク入り飲料の保存時の劣化臭を抑制し、コクやミルク感を増強する方法として、ミルク入り飲料を製造する工程でカルシウム塩を添加する方法が知られている(特開平08-023878号公報)。しかし、本発明の特定量のブドウ糖を配合した乳糖含有コーヒー飲料では、飲料中のカルシウムが多いとブドウ糖の効果を阻害し、かえって風味が悪くなることが判明した。本発明のコーヒー飲料中のカルシウム含量は、26mg/100g以下、好ましくは20mg/100g以下、より好ましくは16mg/100g以下、さらに好ましくは15mg/100g以下、特に好ましくは14mg/100g以下とする。飲料中のカルシウム含量は、カルシウムを減じる処理がなされた乳分を用いることで実現できる。脱カルシウムの方法としては、特に限定されず、調温保持による沈殿法等の公知の方法を採ることができる。 As a method for suppressing the deterioration odor during storage of a milk-containing beverage and enhancing the richness and the feeling of milk, a method of adding a calcium salt in the step of producing a milk-containing beverage is known (Japanese Patent Laid-Open No. 08-023878) ). However, in a lactose-containing coffee beverage containing a specific amount of glucose according to the present invention, it has been found that if the amount of calcium in the beverage is large, the effect of glucose is inhibited and the flavor is worsened. The calcium content in the coffee beverage of the present invention is 26 mg / 100 g or less, preferably 20 mg / 100 g or less, more preferably 16 mg / 100 g or less, still more preferably 15 mg / 100 g or less, particularly preferably 14 mg / 100 g or less. The calcium content in the beverage can be achieved by using milk that has been processed to reduce calcium. The method for decalcification is not particularly limited, and a known method such as a precipitation method by maintaining the temperature can be employed.
 本発明の効果は、乳糖の熱変性だけでなく、乳脂肪の熱や保存に伴う風味変化に対しても有効に作用するので、乳脂肪を含有するコーヒー飲料は、本発明の好ましい態様の一つである。ただし、本発明の効果を確実に発揮させるために、乳脂肪を含有させる場合にも、乳分としての飲料中の乳固形分が3.0重量%未満とすることが重要である。 Since the effect of the present invention is effective not only for heat denaturation of lactose but also for changes in flavor associated with heat and storage of milk fat, a coffee beverage containing milk fat is one of the preferred embodiments of the present invention. One. However, in order to ensure the effect of the present invention, it is important that the milk solid content in the beverage as milk is less than 3.0% by weight even when milk fat is contained.
 [糖類]
 本発明の乳糖含有飲料は、特定量のブドウ糖を配合することを特徴とする。ブドウ糖は、飲料中のブドウ糖含量(b)と、飲料中の乳糖含量(a)の濃度比[(b)/(a)]が0.2以上、好ましくは0.5以上、より好ましくは1.0以上、さらに好ましくは1.5以上、特に好ましくは1.7以上となるように配合する。(b)/(a)の上限は15以下、好ましくは12以下、より好ましくは10以下、さらに好ましくは7.5以下、特に好ましくは6以下である。
[Sugar]
The lactose-containing beverage of the present invention is characterized by blending a specific amount of glucose. For glucose, the concentration ratio [(b) / (a)] of the glucose content (b) in the beverage and the lactose content (a) in the beverage is 0.2 or more, preferably 0.5 or more, more preferably 1 0.0 or more, more preferably 1.5 or more, and particularly preferably 1.7 or more. The upper limit of (b) / (a) is 15 or less, preferably 12 or less, more preferably 10 or less, still more preferably 7.5 or less, and particularly preferably 6 or less.
 本発明は、熱に伴う風味変性の抑制に、熱変性成分として知られているブドウ糖を一定割合で配合させることにより、ミルク入りコーヒー飲料において、高温殺菌及び/又は加温状態での保存による風味の変質が抑制されるという驚くべき知見に基づくものであるが、ブドウ糖含有量(b)が多過ぎると、ブドウ糖自身の熱変性に伴うpH低下による風味変化が大きくなり、本発明の効果を損なうことがある。したがって、ブドウ糖含量は、飲料中0.1~10%、好ましくは0.5~7.0%程度となるように配合するのがよい。 In the present invention, by adding glucose, which is known as a heat-denatured component, at a certain ratio in order to suppress the flavor denaturation due to heat, in a coffee beverage containing milk, the flavor is obtained by high-temperature sterilization and / or storage in a warmed state. However, if the glucose content (b) is too high, a change in flavor due to a decrease in pH due to thermal denaturation of glucose itself increases, impairing the effects of the present invention. Sometimes. Therefore, the glucose content is preferably 0.1 to 10%, preferably 0.5 to 7.0% in the beverage.
 上記のとおり、本発明のコーヒー飲料は、乳糖及びブドウ糖を必須成分として含むものであるが、所望により他の糖類(ショ糖、異性化糖、果糖、麦芽糖、オリゴ糖など)を添加してもよい。ただし、異性化糖、果糖、麦芽糖は、本発明のブドウ糖の効果を阻害することがあるから、配合する場合には、飲料中のブドウ糖含量(b)に対して、0.8以下、好ましくは0.5以下となる量にするのがよい。後述する実施例に記載のとおり、本発明の効果は、飲料中の糖類濃度が1.0~10.0g/100gの範囲で顕著に発現する。ここで、飲料中の糖類濃度とは、飲料中に存在するブドウ糖、乳糖、ショ糖、果糖、麦芽糖の総量をいう。 As described above, the coffee beverage of the present invention contains lactose and glucose as essential components, but other saccharides (sucrose, isomerized sugar, fructose, maltose, oligosaccharide, etc.) may be added as desired. However, since isomerized sugar, fructose, and maltose may inhibit the effect of the glucose of the present invention, when blended, the glucose content in the beverage (b) is 0.8 or less, preferably The amount is preferably 0.5 or less. As described in the examples described later, the effects of the present invention are remarkably exhibited when the sugar concentration in the beverage is in the range of 1.0 to 10.0 g / 100 g. Here, the saccharide concentration in the beverage refers to the total amount of glucose, lactose, sucrose, fructose and maltose present in the beverage.
 [容器詰め飲料]
 本明細書でいう「容器詰め飲料」とは、シングルストレングス、すなわち容器詰飲料を開封後、常態として薄めずにそのまま飲めるものをいい、飲用時に薄めて飲むことが前提である濃縮コーヒーは本発明の範疇から明確に除かれる。
[Contained beverages]
As used herein, “packed beverage” refers to a single strength, that is, a beverage that can be consumed as it is without being diluted as a normal after opening the packaged beverage. Is clearly excluded from the category.
 本発明のコーヒー飲料は、加熱殺菌して製造される容器詰めコーヒー飲料である。本明細書でいう高温殺菌とは、高温で短時間殺菌した後、無菌条件下で殺菌処理された保存容器に充填する方法(UHT殺菌法)と、調合液を缶等の保存容器に充填した後、レトルト処理を行うレトルト殺菌法とをいい、120℃で4分相当(致死値F0=3.1)以上の加熱殺菌をいう。本発明の効果は、加熱殺菌強度が強い方が顕著であるので、レトルト殺菌処理を行う缶入りのコーヒーに好適に適用される。缶入りのコーヒー飲料は、コーヒー分に乳分を配合し、さらにブドウ糖、所望によりその他原料を混合した液を均質処理して金属缶に充填後、食品衛生法に定められた殺菌条件でレトルト殺菌処理して製造することができる。すなわち、本発明の缶入りコーヒー飲料(好ましくは、2-フルフリルメチルジスルフィド、メチル(2-メチル-3-フリル)ジスルフィド、2-フルフリルメチルスルフィドおよびメチル(5-メチル-2-フリル)ジスルフィドからなる群から選択される一以上の香気成分を含む缶入りコーヒー飲料)は、以下の工程1~3を経て製造される。
 コーヒー分に乳糖及びブドウ糖を配合して調合液を得る工程1、
 該調合液を容器に充填する工程2、および
 レトルト殺菌処理する工程3
The coffee beverage of the present invention is a container-packed coffee beverage manufactured by heat sterilization. High temperature sterilization as used in the present specification is a method of filling a storage container sterilized under aseptic conditions after being sterilized at high temperature for a short time (UHT sterilization method), and filling a storage container such as a can with a prepared solution. After that, it is called retort sterilization method in which retort treatment is performed, and it means heat sterilization at 120 ° C. for 4 minutes (lethal value F0 = 3.1) or more. Since the effect of the present invention is more pronounced when the heat sterilization strength is stronger, it can be suitably applied to canned coffee that undergoes retort sterilization. Canned coffee beverages are made by blending milk into coffee and then homogenizing a mixture of glucose and other ingredients, if necessary, and filling into metal cans, followed by retort sterilization under the sterilization conditions stipulated in the Food Sanitation Law. It can be processed and manufactured. That is, the canned coffee beverage of the present invention (preferably 2-furfurylmethyl disulfide, methyl (2-methyl-3-furyl) disulfide, 2-furfurylmethyl sulfide and methyl (5-methyl-2-furyl) disulfide) Canned coffee beverages containing one or more fragrance components selected from the group consisting of: are produced through the following steps 1 to 3.
Step 1 of blending lactose and glucose with coffee to obtain a mixed solution,
Step 2 of filling the preparation liquid into a container, and Step 3 of retort sterilization treatment
 上記の製造方法の工程1において、乳糖(a)に対するブドウ糖(b)の割合(重量比)が0.2以上、好ましくは0.5以上、より好ましくは1.0以上、さらに好ましくは1.5以上、特に好ましくは1.7以上となるように配合する。この上限は15以下、好ましくは12以下、より好ましくは10以下、さらに好ましくは7.5以下、特に好ましくは6以下となるように配合すると、高温殺菌処理後も、飲料中の乳糖とブドウ糖の含有割合がこの範囲内に納まりやすい。 In step 1 of the above production method, the ratio (weight ratio) of glucose (b) to lactose (a) is 0.2 or more, preferably 0.5 or more, more preferably 1.0 or more, and still more preferably 1. It mix | blends so that it may become 5 or more, Most preferably 1.7 or more. When the upper limit is 15 or less, preferably 12 or less, more preferably 10 or less, still more preferably 7.5 or less, and particularly preferably 6 or less, the lactose and glucose in the beverage after the high temperature sterilization treatment. The content ratio tends to fall within this range.
 また、工程1の調合液に、特定量のカフェインを含有させることで、高温殺菌及び/又は加温状態での保存による風味の変質抑制という本発明の効果を、より一層強く発現させることができる。好ましい調合液中のカフェイン含量は、30~95mg/100g、好ましくは45~85mg/100g程度である。調合液中のカフェイン含量は、コーヒー分由来のカフェイン含量を測定し、不足する場合には食品添加物で指定されているカフェイン等を適宜添加して用いることができる。コーヒー飲料中のカフェインの含量は、当業者であれば、例えばHPLCを用いて、適宜測定することができる。 In addition, by incorporating a specific amount of caffeine in the preparation liquid of Step 1, the effect of the present invention of suppressing the alteration of flavor due to high temperature sterilization and / or storage in a warmed state can be expressed more strongly. it can. The caffeine content in the preferred preparation is about 30 to 95 mg / 100 g, preferably about 45 to 85 mg / 100 g. The caffeine content in the preparation liquid can be determined by measuring the caffeine content derived from the coffee component and, if insufficient, caffeine or the like designated by the food additive can be appropriately added and used. A person skilled in the art can appropriately measure the content of caffeine in a coffee beverage using, for example, HPLC.
 さらに、工程1の調合液は、pH調整剤によりpH5.5~8.0程度に調整されていることが好ましい。pHが低いと工程3のレトルト殺菌に伴う加熱臭や雑味による後味の苦味や雑味が顕著となり、本発明の効果を阻害することがある。加熱殺菌した後の内容物のpHが5.5~7.0、好ましくはpH6.0~7.0程度となるように、調合液のpHを調整する。pH調整剤としては、炭酸ナトリウム、炭酸水素ナトリウム、リン酸水素ニナトリウム、炭酸カリウム等が挙げられるが、本発明の効果の観点からアルカリ性ナトリウム塩又はアルカリ性カリウム塩が好ましく、特に炭酸水素ナトリウムが最も好ましい。pHが中性領域に調整されたミルク入り飲料では、コーヒー分が本来有するほのかな酸味等の風味や味わいが失われやすい、焙煎コーヒー特有の焙煎香気(ロースト感)が知覚しにくいという問題があるが、本発明のブドウ糖を配合したミルク入り飲料では、コーヒー分が本来有するコーヒー風味が増強され、ミルク風味とコーヒー風味がバランスよく調和したコーヒー飲料となる。 Furthermore, it is preferable that the preparation liquid in step 1 is adjusted to about pH 5.5 to 8.0 with a pH adjuster. When pH is low, the aftertaste bitterness and miscellaneous taste by the heating odor and miscellaneous taste accompanying the retort sterilization of process 3 will become remarkable, and the effect of this invention may be inhibited. The pH of the preparation is adjusted so that the content after heat sterilization has a pH of 5.5 to 7.0, preferably about 6.0 to 7.0. Examples of the pH adjuster include sodium carbonate, sodium hydrogen carbonate, disodium hydrogen phosphate, potassium carbonate, and the like. From the viewpoint of the effect of the present invention, an alkaline sodium salt or an alkaline potassium salt is preferable, and sodium hydrogen carbonate is most preferable. preferable. In beverages containing milk whose pH is adjusted to a neutral range, it is difficult to perceive the roasted aroma (roasted) peculiar to roasted coffee, and the flavor and taste such as the subtle acidity that the coffee component originally has is easily lost. However, in the beverage with milk blended with the glucose of the present invention, the coffee flavor inherent in the coffee component is enhanced, and the coffee beverage has a balanced milk flavor and coffee flavor.
 その他、本発明のコーヒー飲料には、所望によりその他原料を配合することができる。その他原料としては、カゼインナトリウム等の安定剤、抗酸化剤、乳化剤、香料等が挙げられる。 In addition, other ingredients can be blended in the coffee beverage of the present invention as desired. Other raw materials include stabilizers such as sodium caseinate, antioxidants, emulsifiers, and fragrances.
 以下、実施例に基づいて本発明を説明するが、本発明はこれらの実施例に限定されるものではない。また、本明細書において特記しない限り、濃度等は重量基準であり、数値範囲はその端点を含むものとして記載される。また、実施例における各種成分濃度は、以下の方法で測定した。 Hereinafter, the present invention will be described based on examples, but the present invention is not limited to these examples. Further, unless otherwise specified in the present specification, the concentration and the like are based on weight, and the numerical range is described as including the end points. Moreover, the various component density | concentration in an Example was measured with the following method.
 (糖類濃度の測定)
 飲料中のブドウ糖濃度及び乳糖濃度は、HPLC糖分析装置(LC-20AD株式会社  島津製作所社製)を以下の条件で操作し、検量線法により定量して測定した。
・カラム:Inertsil NH2,φ3mm×150mm[ジーエルサイエンス株式会社]
・カラム温度:室温
・移動相:アセトニトリル:水=8:2
・流量:0.7ml/min
・注入量:5μL
・検出:示差屈折計 RID-10A [株式会社  島津製作所] 
(Measurement of sugar concentration)
The glucose concentration and the lactose concentration in the beverage were measured by operating a HPLC sugar analyzer (manufactured by Shimadzu Corporation, LC-20AD Co., Ltd.) under the following conditions and quantifying by a calibration curve method.
・ Column: Inertsil NH 2, φ3mm × 150mm [GL Sciences Inc.]
-Column temperature: Room temperature-Mobile phase: Acetonitrile: Water = 8: 2
・ Flow rate: 0.7ml / min
・ Injection volume: 5μL
・ Detection: Differential refractometer RID-10A [Shimadzu Corporation]
 (カフェイン濃度の測定)
 飲料中のカフェイン濃度は、コーヒー飲料を移動相Aで10倍希釈(w/w)した後、メンブランフィルター(ADVANTEC製 Cellulose Acetate 0.45μm)で濾過し、HPLCに注入して定量した。HPLCの測定条件は以下のとおり。
・カラム:TSK-gel ODS-80TsQA(4.6mmφx150mm、東ソー株式会社)
・移動相:A:水:トリフルオロ酢酸=1000:0.5
          B:アセトニトリル:トリフルオロ酢酸=1000:0.5
・流速:1.0ml/min
・カラム温度:40℃
・グラディエント条件;分析開始から5分後まではA液100%保持、 
  5分から10分まででB液7.5%、
  10分から20分まででB液10.5%、
  20分から32分までB液10.5%保持、
  32分から45分まででB液26.3%、
  45分から46分まででB液75.0%、
  46分から51分までB液75.0%保持、
  51分から52分まででB液0%
  52分から58分までB液0%保持、
・注入量:5.0μl
・検出波長:280nm
・リテンションタイム:19.3分
・標準物質:カフェイン(無水)(ナカライテスク株式会社)
(Measurement of caffeine concentration)
The caffeine concentration in the beverage was quantified by diluting the coffee beverage 10-fold with mobile phase A (w / w), filtering with a membrane filter (Cellulose Acetate 0.45 μm manufactured by ADVANTEC), and injecting it into HPLC. The HPLC measurement conditions are as follows.
Column: TSK-gel ODS-80TsQA (4.6mmφx150mm, Tosoh Corporation)
-Mobile phase: A: Water: Trifluoroacetic acid = 1000: 0.5
B: Acetonitrile: trifluoroacetic acid = 1000: 0.5
・ Flow rate: 1.0ml / min
・ Column temperature: 40 ℃
-Gradient conditions: 100% solution A was retained until 5 minutes after the start of analysis.
Liquid B 7.5% in 5 to 10 minutes,
B liquid 10.5% from 10 to 20 minutes,
B liquid 10.5% retention from 20 to 32 minutes,
Liquid B 26.3% from 32 to 45 minutes
Liquid B 75.0% from 45 to 46 minutes,
Retain 75.0% of B liquid from 46 to 51 minutes,
Liquid B 0% from 51 to 52 minutes
B liquid 0% retention from 52 to 58 minutes,
・ Injection volume: 5.0μl
・ Detection wavelength: 280nm
・ Retention time: 19.3 minutes ・ Reference material: Caffeine (anhydrous) (Nacalai Tesque)
 (クロロゲン酸濃度の測定)
 飲料中のクロロゲン酸濃度は、コーヒー飲料を移動相Aで10倍希釈(w/w)した後、メンブランフィルター(ADVANTEC製 Cellulose Acetate 0.45μm)で濾過し、HPLCに注入して定量した。HPLCの測定条件は以下のとおり。
・カラム:ガードカラムInertsil ODS-3(4.0mmφx20mm)+Inertsil ODS-2(4.6mmφx250mm)(GLサイエンス株式会社)
・移動相:A:0.05M 酢酸、0.1mM 1-ヒドロキシエタン-1,1-ジホスホン酸、3(V/V)%アセトニトリル溶液
      B:0.05M 酢酸、0.1mM 1-ヒドロキシエタン-1,1-ジホスホン酸、97(V/V)%アセトニトリル溶液
・流速:1.0ml/min
・カラム温度:35℃
・グラディエント条件;分析開始から1分後まではA液100%保持、 
  1分から20分まででB液13%、
  20分から25分まででB液13%保持、
  25分から27分まででB液15%、
  27分から45分までB液15%保持、
  45分から55分まででB液20%、
  55分から60分まででB液100%
  60分から70分までB液100%保持、
  70分から75分までA液100%、
  75分から100分までA液100%保持
・注入量:10.0μl
・検出波長:325nm
・標準物質:クロロゲン酸0.5水和物(含量99%)(和光一級)
(Measurement of chlorogenic acid concentration)
The chlorogenic acid concentration in the beverage was quantified by diluting the coffee beverage 10-fold with mobile phase A (w / w), filtering with a membrane filter (Cellulose Acetate 0.45 μm manufactured by ADVANTEC), and injecting it into HPLC. The HPLC measurement conditions are as follows.
・ Column: Guard column Inertsil ODS-3 (4.0mmφx20mm) + Inertsil ODS-2 (4.6mmφx250mm) (GL Science Co., Ltd.)
Mobile phase: A: 0.05 M acetic acid, 0.1 mM 1-hydroxyethane-1,1-diphosphonic acid, 3 (V / V)% acetonitrile solution B: 0.05 M acetic acid, 0.1 mM 1-hydroxyethane-1,1- Diphosphonic acid, 97 (V / V)% acetonitrile solution, flow rate: 1.0 ml / min
・ Column temperature: 35 ℃
-Gradient conditions: 100% solution A is retained from 1 minute after the start of analysis.
From 1 minute to 20 minutes, B solution 13%,
B liquid 13% retention from 20 to 25 minutes,
From 25 minutes to 27 minutes, B solution 15%,
B liquid 15% retention from 27 to 45 minutes,
From 45 minutes to 55 minutes, B liquid 20%,
Liquid B 100% in 55 to 60 minutes
Hold B solution 100% from 60 to 70 minutes,
From 70 minutes to 75 minutes, solution A is 100%,
75% to 100 minutes A solution 100% retention and injection volume: 10.0 μl
・ Detection wavelength: 325nm
Reference material: Chlorogenic acid hemihydrate (content 99%) (Wako first grade)
 (実施例1)
  コーヒー分として、コロンビア産のコーヒー豆から製造された既存のコーヒーエキスを用いた。コーヒーエキスに牛乳113mL/Lを配合し、さらにコーヒー固形分が1.55%となるように水で希釈して、500メッシュで濾過して不溶性固形分を除き、使用した(以下、コーヒー調合液という)。
(Example 1)
As coffee, an existing coffee extract produced from Colombian coffee beans was used. The coffee extract was blended with 113 mL / L of milk, diluted with water so that the coffee solid content was 1.55%, filtered through 500 mesh to remove insoluble solid content, and used (hereinafter referred to as coffee blending solution). Called).
 このコーヒー調合液(乳糖:0.5g/100g、カフェイン:53mg/100g含有)に、ブドウ糖とショ糖が総量56.0g/Lとなるように種々の割合で配合し、pH調整剤(炭酸水素ナトリウム)を用いてpHを6.7に調整し、この液を190mLずつ缶に充填してレトルト殺菌処理を行った(サンプルNo.2~7)。また、対照として、ブドウ糖を配合せずにショ糖のみを用いること以外は同様にして缶入りコーヒー飲料を調製した(サンプルNo.1)。 In this coffee preparation liquid (lactose: 0.5 g / 100 g, caffeine: 53 mg / 100 g contained), glucose and sucrose are blended in various proportions so that the total amount is 56.0 g / L, and a pH adjuster (carbonic acid) Sodium hydride) was used to adjust the pH to 6.7, and this solution was filled into cans in 190 mL portions and subjected to retort sterilization (Sample Nos. 2 to 7). As a control, a canned coffee drink was prepared in the same manner except that only sucrose was used without adding glucose (sample No. 1).
 得られた容器詰めコーヒー飲料(乳固形分:1.49g/100g、糖類濃度:6.0g/100g、pH6.3)について、専門パネルによる官能評価を行った。評価は、トップの香ばしい香り(トップ香)の強さ、後味に感じる香ばしい香り(ラスト香)の強さについて、No.1を対照(3点)として、5点:対照よりもとても強い(好ましい)、4点:対照よりも強い(好ましい)、3点:対照と同程度、2点:対照よりも弱い(好ましくない)、1点:対照よりもとても弱い(好ましくない)で各人が評価した結果から平均点を算出した。また、乳劣化臭の強さについて、No.1を対照(3点)として、5点:対照よりもとても弱い(好ましい)、4点:対照よりも弱い(好ましい)、3点:対照と同程度、2点:対照よりも強い(好ましくない)、1点:対照よりもとても強い(好ましくない)で各人が評価した結果から平均点を算出した。さらに、総合的な好ましさについても同様に評価した。 The obtained container-packed coffee beverage (milk solid content: 1.49 g / 100 g, sugar concentration: 6.0 g / 100 g, pH 6.3) was subjected to sensory evaluation by a specialized panel. Evaluation is No. 1 as the control (3 points) for the strength of the fragrant fragrance (top fragrance) at the top and the intensity of the fragrant fragrance (last fragrance) felt in the aftertaste. ) 4 points: stronger than control (preferred), 3 points: comparable to control, 2 points: weaker than control (not preferable), 1 point: very weaker than control (not preferable) The average score was calculated from the results. In addition, regarding the strength of milk deterioration odor, No. 1 as a control (3 points), 5 points: very weaker than control (preferred), 4 points: weaker than control (preferred), 3 points: same as control The average score was calculated from the results of each person's evaluation of degree, 2 points: stronger than the control (preferably), 1 point: very strong (preferably) than the control. Furthermore, the overall preference was evaluated in the same way.
 結果を表1に示す。乳糖(a)に対するブドウ糖(b)の割合[(b)/(a)]が、0.2以上、好ましくは0.3以上となるようにブドウ糖を配合することによって、高温殺菌に伴う乳劣化臭が抑制されることが明らかとなった。また、特定量のブドウ糖を配合することによってコーヒー風味も増強され、良好な乳風味とコーヒー風味のバランスの取れたドリンカビリティの高いミルク入りコーヒー飲料となった。ここで、本明細書でいうドリンカビリティとは、毎日摂取可能な嗜好性(ドリンカビリティ;飲料の性質を指し、ある飲料を一定量飲用した後も、なおおいしく飲み続けられる場合には、その飲料はドリンカビリティがあるといえる。ドリンカビリティは「飲みたいかどうか」と表現されることもある。)を表わす。 The results are shown in Table 1. Milk degradation associated with high temperature sterilization by blending glucose so that the ratio of glucose (b) to lactose (a) [(b) / (a)] is 0.2 or more, preferably 0.3 or more It became clear that the odor was suppressed. In addition, the coffee flavor was enhanced by adding a specific amount of glucose, resulting in a milk drink with high drinkability and a good balance between milk flavor and coffee flavor. Here, the drinkability in the present specification refers to the preference (drinkability; the nature of the beverage) that can be taken every day, and after a certain amount of beverage has been drunk, it can continue to be drunk. It can be said that the drink has drinkability, which is sometimes expressed as “whether or not you want to drink”.)
Figure JPOXMLDOC01-appb-T000001
Figure JPOXMLDOC01-appb-T000001
 (実施例2)
 牛乳を遠心分離して脂肪分の多いクリームと脂肪分の少ない脱脂乳に分離した。この脱脂乳をイオン交換樹脂を充填した筒に通過させてカルシウムイオンを除去した後、これを遠心分離して得られたクリーム分と混合し、この液を乾燥させて、脱カルシウム粉乳を得た。
(Example 2)
The milk was centrifuged and separated into fatty cream and lean skimmed milk. After passing this skimmed milk through a tube filled with an ion exchange resin to remove calcium ions, this was mixed with the cream obtained by centrifuging, and this liquid was dried to obtain decalcified powdered milk. .
 実施例1における乳分を、表2に示す割合の牛乳と脱カルシウム粉乳の併用に変えること以外は、実施例1のNo.5の飲料と同様にして缶入りのレトルト殺菌コーヒー飲料を製造した。 No. of Example 1 except that the milk content in Example 1 is changed to the combined use of milk and decalcified milk powder in the proportions shown in Table 2. A canned retort sterilized coffee beverage was produced in the same manner as the beverage of No. 5.
Figure JPOXMLDOC01-appb-T000002
Figure JPOXMLDOC01-appb-T000002
 得られた缶入りコーヒー飲料(乳固形分:1.49g/100g、糖類濃度:6.0g/100g、pH6.3)について、実施例1と同様に評価した。結果を表3に示す。いずれもコーヒー風味も増強され、良好な乳風味とコーヒー風味のバランスの取れたドリンカビリティの高いミルク入りコーヒー飲料であったが、表3より明らかなように、乳糖が同量のコーヒー飲料において、飲料中のカルシウム濃度が低くなるにしたがって乳劣化臭がより低減でき、コーヒーの香りが増強されることが示唆された。 The obtained canned coffee beverage (milk solid content: 1.49 g / 100 g, sugar concentration: 6.0 g / 100 g, pH 6.3) was evaluated in the same manner as in Example 1. The results are shown in Table 3. In both cases, the coffee flavor was enhanced, and it was a drinkable milk coffee beverage with a good balance between milk flavor and coffee flavor. It was suggested that as the calcium concentration in the beverage decreases, the milk deterioration odor can be further reduced, and the coffee aroma is enhanced.
Figure JPOXMLDOC01-appb-T000003
Figure JPOXMLDOC01-appb-T000003
 (実施例3)
 ブラジル産コーヒー豆(L値20程度)を粉砕機(日本グラニュレーター社製)で粉砕し、94℃の熱水でドリップしてBrix2.9のコーヒー抽出液を得、500メッシュで濾過して不溶性固形分を除いた。このコーヒー抽出液500g/Lに、ブドウ糖とショ糖を総量59.0~74.0g/L(加熱による分解を見越した量)となるように種々の割合で配合し、さらに乳分(牛乳55mL/L)を配合して、全量が1Lとなるように水を添加した後、pH調整剤(炭酸水素ナトリウム)を用いてpHを6.7に調整し、この液を190mLずつ缶に充填してレトルト殺菌処理を行った(サンプルNo.11~15)。また、対照として、ブドウ糖を配合せずにショ糖のみを用いること以外は同様にして缶入りコーヒー飲料を調製した(サンプルNo.10)。
(Example 3)
Brazilian coffee beans (L value of about 20) are pulverized with a pulverizer (manufactured by Nippon Granulator Co., Ltd.), drip with 94 ° C hot water to obtain a coffee extract of Brix 2.9, filtered through 500 mesh and insoluble Solids were removed. Into this coffee extract 500 g / L, glucose and sucrose are blended in various proportions so that the total amount is 59.0-74.0 g / L (amount in anticipation of decomposition by heating), and milk (55 mL of milk) is added. / L), water is added so that the total amount is 1 L, pH is adjusted to 6.7 using a pH adjuster (sodium bicarbonate), and this solution is filled into cans in 190 mL portions. Then, retort sterilization was performed (Sample Nos. 11 to 15). As a control, a canned coffee drink was prepared in the same manner except that only sucrose was used without adding glucose (sample No. 10).
 得られた缶入りコーヒー飲料(クロロゲン酸類:63mg/100g、乳固形分:0.72g/100g、糖類濃度:6.0g/100g、pH6.3)について、実施例1と同様に対照と比べた官能評価を行った。結果を表4に示す。 The obtained canned coffee beverage (chlorogenic acids: 63 mg / 100 g, milk solid content: 0.72 g / 100 g, sugar concentration: 6.0 g / 100 g, pH 6.3) was compared with the control in the same manner as in Example 1. Sensory evaluation was performed. The results are shown in Table 4.
Figure JPOXMLDOC01-appb-T000004
Figure JPOXMLDOC01-appb-T000004
 (実施例4)
 実施例3のコーヒー抽出液に、ブドウ糖とショ糖が総量56.0~70.0g/L(加熱による分解を見越した量)となるように種々の割合で配合し、さらに乳分(牛乳135mL/L)とする以外は、実施例3と同様にして缶入りコーヒー飲料を製造した。得られた缶入りコーヒー飲料(クロロゲン酸類:63mg/100g、乳固形分:1.78g/100g、糖類濃度:6.0g/100g、pH6.3)について、実施例3と同様に官能評価を行った。結果を表5に示す。
Example 4
In the coffee extract of Example 3, glucose and sucrose are blended in various proportions so that the total amount is 56.0 to 70.0 g / L (amount in anticipation of decomposition by heating), and milk (135 mL of milk) is further added. / L) A canned coffee drink was produced in the same manner as in Example 3. The obtained canned coffee beverage (chlorogenic acids: 63 mg / 100 g, milk solid content: 1.78 g / 100 g, sugar concentration: 6.0 g / 100 g, pH 6.3) was subjected to sensory evaluation in the same manner as in Example 3. It was. The results are shown in Table 5.
Figure JPOXMLDOC01-appb-T000005
Figure JPOXMLDOC01-appb-T000005
 (実施例5)
 実施例3のコーヒー抽出液に、ブドウ糖とショ糖が総量52.0~65.0g/L(加熱による分解を見越した量)となるように種々の割合で配合し、さらに乳分(牛乳185mL/L)とする以外は、実施例3と同様にして缶入りコーヒー飲料を製造した。得られた缶入りコーヒー飲料(クロロゲン酸類:63mg/100g、乳固形分:2.81g/100g、糖類濃度:6.0g/100g、pH6.3)について、実施例3と同様に官能評価を行った。結果を表6に示す。表4~6より、飲料中のブドウ糖含量(b)と、飲料中の乳糖含量(a)の濃度比[(b)/(a)]が0.2以上となるようにブドウ糖を配合すると、トップ香、ラスト香、乳劣化臭の評価項目全てにおいて、ブドウ糖を配合しなかった場合と比較して改善する傾向がみられ、乳糖由来のオフフレーバーが低減され、コーヒー風味が増強されたドリンカビリティの高いミルク入りコーヒー飲料となった。特に、(b)/(a)が1.7以上となるようにブドウ糖を配合すると、評価点が全て4点を超え、対照と比べて顕著に改善されていると専門パネル全員が評価した。一方、(b)/(a)が30以上となるものは、トップ香、ラスト香、乳劣化臭の評価では改善がみられるものの、濃厚感(ボディ感ともいう)が著しく不足し、総合評価では劣る結果となった。これより、(b)/(a)は0.2~15とすることが示唆された。
(Example 5)
In the coffee extract of Example 3, glucose and sucrose are blended in various proportions so that the total amount is 52.0 to 65.0 g / L (amount in anticipation of decomposition by heating), and milk (185 mL of milk) is further added. / L) A canned coffee drink was produced in the same manner as in Example 3. The obtained canned coffee beverage (chlorogenic acids: 63 mg / 100 g, milk solid content: 2.81 g / 100 g, sugar concentration: 6.0 g / 100 g, pH 6.3) was subjected to sensory evaluation in the same manner as in Example 3. It was. The results are shown in Table 6. From Tables 4-6, when glucose is added so that the concentration ratio [(b) / (a)] of the glucose content (b) in the beverage and the lactose content (a) in the beverage is 0.2 or more, A drinker with a tendency to improve compared to the case where glucose was not added in all evaluation items of top aroma, last aroma and milk deterioration odor, reduced off-flavor derived from lactose, and enhanced coffee flavor It became a coffee drink with milk with high ability. In particular, when glucose was added so that (b) / (a) was 1.7 or more, all the evaluation points exceeded 4 points, and all the expert panels evaluated that the improvement was markedly improved compared to the control. On the other hand, when (b) / (a) is 30 or more, the top odor, last fragrance, and milk deterioration odor are improved, but the richness (also called body sensation) is remarkably insufficient. Inferior results. This suggests that (b) / (a) is 0.2-15.
Figure JPOXMLDOC01-appb-T000006
Figure JPOXMLDOC01-appb-T000006
 (実施例6)
 実施例3のコーヒー抽出液に、ブドウ糖40g/L、ショ糖63g/Lを配合し、さらに乳分(牛乳135mL/L)とする以外は、実施例3と同様にして缶入りコーヒー飲料を製造した(サンプルNo.29)。また、対照として、ブドウ糖を配合せずにショ糖95g/Lを配合した缶入りコーヒー飲料を製造した(サンプルNo.28)。得られた缶入りコーヒー飲料(クロロゲン酸類:63mg/100g、乳固形分:1.78g/100g、糖類濃度:9.7g/100g、pH6.3)について、対照と比較した官能評価を行った。結果を表7に示す。
(Example 6)
A canned coffee beverage is produced in the same manner as in Example 3 except that 40 g / L of glucose and 63 g / L of sucrose are added to the coffee extract of Example 3 and the milk content (milk 135 mL / L) is further added. (Sample No. 29). Moreover, the canned coffee drink which mix | blended sucrose 95g / L without mix | blending glucose was manufactured as a control | contrast (sample No. 28). The obtained canned coffee beverage (chlorogenic acids: 63 mg / 100 g, milk solid content: 1.78 g / 100 g, sugar concentration: 9.7 g / 100 g, pH 6.3) was subjected to sensory evaluation compared to the control. The results are shown in Table 7.
Figure JPOXMLDOC01-appb-T000007
Figure JPOXMLDOC01-appb-T000007
 (実施例7)
 ブドウ糖及びショ糖の配合量を4g/L、7g/Lとし、さらに乳分(牛乳130mL/L)とする以外は、実施例6と同様にして缶入りコーヒー飲料を製造した(サンプルNo.31)。また、対照として、ブドウ糖を配合せずにショ糖10g/Lを配合した缶入りコーヒー飲料を製造した(サンプルNo.30)。得られた缶入りコーヒー飲料(クロロゲン酸類:63mg/100g、乳固形分:1.71g/100g、糖類濃度:1.5g/100g、pH6.3)について、対照と比較した官能評価を行った。結果を表8に示す。表7及び8から、糖類濃度にかかわらず、飲料中の乳糖含量(a)の濃度比[(b)/(a)]が0.2~15となるようにブドウ糖を配合したコーヒー飲料は、ブドウ糖を配合していないものと比較して、トップ香、ラスト香、乳劣化臭のいずれの評価項目においても上回り、パネル全員が対照と比較して好ましい、ドリンカビリティの高い飲料であると評価した。
(Example 7)
A canned coffee beverage was produced in the same manner as in Example 6 except that the amounts of glucose and sucrose were 4 g / L and 7 g / L, and the milk content (milk 130 mL / L) was used (Sample No. 31). ). Moreover, the canned coffee drink which mix | blended 10g / L of sucrose without mix | blending glucose was manufactured as a control | contrast (sample No. 30). The obtained canned coffee beverage (chlorogenic acids: 63 mg / 100 g, milk solid content: 1.71 g / 100 g, sugar concentration: 1.5 g / 100 g, pH 6.3) was subjected to sensory evaluation as compared with the control. The results are shown in Table 8. From Tables 7 and 8, regardless of the saccharide concentration, the coffee beverage blended with glucose so that the concentration ratio [(b) / (a)] of the lactose content (a) in the beverage is 0.2 to 15, Compared to those that do not contain glucose, the top odor, last fragrance, and milk deterioration odor exceeded all the evaluation items, and all of the panels were evaluated as being highly drinkable beverages that were preferable to the control. did.
Figure JPOXMLDOC01-appb-T000008
Figure JPOXMLDOC01-appb-T000008
 (実施例8)
 実施例5のNo.23のブドウ糖を0.19g/100gとし、乳分として脱脂粉乳及び実施例2で調製した脱カルシウム粉乳の配合割合を変えること以外は、実施例5と同様にして缶入りコーヒー飲料を製造した。得られた缶入りコーヒー飲料(クロロゲン酸類:63mg/100g、乳固形分:2.05~2.81g/100g、糖類濃度:5.9g/100g、pH6.3)について、ブドウ糖を配合していない対照(サンプルNo.22)と比較した官能評価を行った。
(Example 8)
No. 5 in Example 5. A canned coffee drink was produced in the same manner as in Example 5 except that the glucose of 23 was 0.19 g / 100 g, and the blending ratio of skim milk powder and the decalcified milk powder prepared in Example 2 was changed as milk. The resulting canned coffee beverage (chlorogenic acids: 63 mg / 100 g, milk solids: 2.05 to 2.81 g / 100 g, sugar concentration: 5.9 g / 100 g, pH 6.3) does not contain glucose Sensory evaluation was performed in comparison with the control (sample No. 22).
 結果を表9に示す。飲料中の乳糖含量(a)の濃度比[(b)/(a)]が0.2以上となるコーヒー飲料は、トップ香、ラスト香、乳劣化臭のいずれの評価項目においても改善が見られ、ドリンカビリティの高い飲料となったが、特に、ブドウ糖及び乳糖含量が同じ、すなわち(b)/(a)が同じ場合には、カルシウム濃度が少ないほど乳劣化臭がより低減でき、コーヒーの香りが増強された。 The results are shown in Table 9. Coffee beverages with a concentration ratio [(b) / (a)] of lactose content (a) in the beverage of 0.2 or more show improvement in any of the evaluation items of top fragrance, last fragrance, and milk odor. In particular, when the glucose and lactose content is the same, that is, when (b) / (a) is the same, the lower the calcium concentration, the lower the milk deterioration odor. The scent of was enhanced.
Figure JPOXMLDOC01-appb-T000009
Figure JPOXMLDOC01-appb-T000009
 (実施例9)
 市販のミルク入り飲料(無糖タイプ、甘味料(アセスルファムカリウム)配合)を用い、これにブドウ糖5.5g/Lの濃度で配合して0.53g/100gを配合して得られる液(サンプルNo.37)を、市販のミルク入り飲料を対照(サンプルNo.36)として官能評価を行った。結果を表10に示す。ブドウ糖を配合した飲料では、好ましいコーヒーの風味が増強され、一層ドリンカビリティの高い飲料となった。
Example 9
Using a commercially available milk beverage (sugar-free type, sweetener (acesulfame potassium) blended), blended at a concentration of 5.5 g / L glucose and 0.53 g / 100 g (sample No.) 37) was subjected to sensory evaluation using a commercially available milk beverage as a control (sample No. 36). The results are shown in Table 10. In beverages containing glucose, the preferred coffee flavor was enhanced and beverages with higher drinkability were obtained.
Figure JPOXMLDOC01-appb-T000010
Figure JPOXMLDOC01-appb-T000010
 (比較例)
 実施例1のNo.6の飲料について、ブドウ糖を果糖又は還元麦芽糖に変えて、同様に容器詰めコーヒー飲料を製造した(No.38,39)。評価結果を表11に示す。果糖の添加で乳劣化臭は抑制できる傾向にあったが、コーヒーのトップ香やラスト香が損なわれ、乳風味とコーヒー風味のバランスを欠く飲料となった。還元麦芽糖の添加では、乳劣化臭、コーヒー風味の増強ともに効果はなかった。
(Comparative example)
No. of Example 1 With respect to the beverage No. 6, glucose was changed to fructose or reduced maltose, and a container-packed coffee beverage was similarly produced (No. 38, 39). The evaluation results are shown in Table 11. Addition of fructose tended to suppress the milk deterioration odor, but the top and last scents of coffee were impaired, resulting in a beverage lacking a balance between milk flavor and coffee flavor. The addition of reduced maltose had no effect on milk odor and coffee flavor enhancement.
Figure JPOXMLDOC01-appb-T000011
 
Figure JPOXMLDOC01-appb-T000011
 

Claims (3)

  1.  コーヒー分に乳糖及びブドウ糖を配合して得られる調合液を高温殺菌して得られるコーヒー飲料であって、以下
    (i)飲料中の乳糖含量(a)が、0.2~0.9g/100g
    (ii)飲料中の乳糖含量(a)とブドウ糖含量(b)の濃度比[(b)/(a)]が、0.2~15
    (iii)乳固形分が3.0重量%未満
    を満たす前記飲料。
    A coffee beverage obtained by high-temperature sterilization of a liquid mixture obtained by blending lactose and glucose into a coffee component, wherein (i) the lactose content (a) in the beverage is 0.2 to 0.9 g / 100 g
    (Ii) The concentration ratio [(b) / (a)] of the lactose content (a) and the glucose content (b) in the beverage is 0.2-15.
    (Iii) The beverage having a milk solid content of less than 3.0% by weight.
  2.  ミルク入りコーヒー飲料である、請求項1に記載の飲料。 The beverage according to claim 1, which is a coffee beverage containing milk.
  3.  飲料中の乳糖含量(a)とブドウ糖含量(b)の濃度比[(b)/(a)]が1.7~12である、請求項1又は2に記載の飲料。
     
    The beverage according to claim 1 or 2, wherein the concentration ratio [(b) / (a)] of the lactose content (a) and the glucose content (b) in the beverage is 1.7 to 12.
PCT/JP2015/069517 2014-07-07 2015-07-07 Lactose-containing coffee beverage WO2016006603A1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2014-140077 2014-07-07
JP2014140077 2014-07-07
JP2014-165544 2014-08-15
JP2014165544A JP2016026473A (en) 2014-07-07 2014-08-15 Coffee drink containing lactose

Publications (1)

Publication Number Publication Date
WO2016006603A1 true WO2016006603A1 (en) 2016-01-14

Family

ID=55064233

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2015/069517 WO2016006603A1 (en) 2014-07-07 2015-07-07 Lactose-containing coffee beverage

Country Status (2)

Country Link
JP (1) JP2016026473A (en)
WO (1) WO2016006603A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110602953A (en) * 2017-05-18 2019-12-20 花王株式会社 Food composition

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0231664A (en) * 1988-07-19 1990-02-01 Dai Ichi Kogyo Seiyaku Co Ltd Emulsion stabilizer for coffee milk beverage distributed at ordinary temperature and coffee milk beverage, distributed at ordinary temperature and blended with the same stabilizer
JPH0523134A (en) * 1991-07-23 1993-02-02 Mitsubishi Kasei Corp Promoter for enhancing bone strength and foods containing the same
JPH09205990A (en) * 1995-11-27 1997-08-12 Japan Tobacco Inc Manufacture of coffee beverage with milk
JPH119190A (en) * 1997-06-26 1999-01-19 Japan Tobacco Inc Milk-containing coffee beverage and its production
JP2009153440A (en) * 2007-12-26 2009-07-16 Kirin Holdings Co Ltd Packaged black coffee beverage and method for producing the same
WO2012128347A1 (en) * 2011-03-24 2012-09-27 株式会社明治 Coffee beverage and process for producing same

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5237520B2 (en) * 2004-05-28 2013-07-17 味の素ゼネラルフーヅ株式会社 Chlorogenic acid-containing beverage
JP2006023042A (en) * 2004-07-09 2006-01-26 Matsushita Electric Ind Co Ltd Refrigerator
JP2014113059A (en) * 2012-11-14 2014-06-26 Matsutani Chem Ind Ltd Method for imparting rich taste to food and drink

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0231664A (en) * 1988-07-19 1990-02-01 Dai Ichi Kogyo Seiyaku Co Ltd Emulsion stabilizer for coffee milk beverage distributed at ordinary temperature and coffee milk beverage, distributed at ordinary temperature and blended with the same stabilizer
JPH0523134A (en) * 1991-07-23 1993-02-02 Mitsubishi Kasei Corp Promoter for enhancing bone strength and foods containing the same
JPH09205990A (en) * 1995-11-27 1997-08-12 Japan Tobacco Inc Manufacture of coffee beverage with milk
JPH119190A (en) * 1997-06-26 1999-01-19 Japan Tobacco Inc Milk-containing coffee beverage and its production
JP2009153440A (en) * 2007-12-26 2009-07-16 Kirin Holdings Co Ltd Packaged black coffee beverage and method for producing the same
WO2012128347A1 (en) * 2011-03-24 2012-09-27 株式会社明治 Coffee beverage and process for producing same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
AYA KAGAWA ET AL., YONTEI SHOKUHIN SEIBUNHYO, 1994, pages 196 - 197, ISBN: 4-7895-0194-9 *
KUNIO YAMAUCHI ET AL., MILK SOGO JITEN, 1998, pages 46 - 50, ISBN: 4-254-43048-5 *

Also Published As

Publication number Publication date
JP2016026473A (en) 2016-02-18

Similar Documents

Publication Publication Date Title
US7824724B2 (en) Milk-added coffee beverage with basic amino acids
JP7179704B2 (en) Packaged coffee beverage containing furfuryl methyl sulfide
AU2009287839B2 (en) Milk composition and milk-added beverage, both having lower whey protein content
WO2015097935A1 (en) Packaged milk-containing black tea beverage
JP6392966B1 (en) Containerized coffee beverages containing furfuryl methyl sulfide
JP2017163864A (en) Packaged concentrated coffee beverage
WO2016006603A1 (en) Lactose-containing coffee beverage
JP7417505B2 (en) Heat-sterilized beverage containing milk
JP6838107B2 (en) Coffee beverage containing lactose
JP2018153100A (en) Container-packed concentrated coffee drink
JP4112087B2 (en) Coffee production method
JP7174733B2 (en) Dairy-containing beverage
JP2021007373A (en) Composition for instant coffee beverage, and production method of the same
JP7098078B2 (en) Heat-sterilized beverage with milk
JP6480063B2 (en) Heat-treated coffee beverage
JP7408285B2 (en) Coffee drinks and methods for improving the flavor of coffee drinks
JP6366798B1 (en) Heat-treated coffee beverage
JP2023180763A (en) Packed milk-containing coffee drink, and method for producing the same
JP2023015573A (en) Packaged coffee beverage
JP2022029542A (en) Milk-containing beverage
JP6385801B2 (en) Packaged beverages containing milk and plant extracts
JP2022102420A (en) Ready-to-drink milk-containing beverage
WO2015030253A1 (en) Milk-containing coffee beverage with little coffee lipid content
JP2022115178A (en) Coffee beverage
JP2022122397A (en) Coffee beverage and method of producing the same

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 15819023

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 15819023

Country of ref document: EP

Kind code of ref document: A1