JP7417505B2 - Heat-sterilized beverage containing milk - Google Patents

Heat-sterilized beverage containing milk Download PDF

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JP7417505B2
JP7417505B2 JP2020178370A JP2020178370A JP7417505B2 JP 7417505 B2 JP7417505 B2 JP 7417505B2 JP 2020178370 A JP2020178370 A JP 2020178370A JP 2020178370 A JP2020178370 A JP 2020178370A JP 7417505 B2 JP7417505 B2 JP 7417505B2
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milk
beverage
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sugar
gaba
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JP2022069277A (en
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沙織 結城
鷹明 谷
範之 神崎
透 片山
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Suntory Holdings Ltd
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本発明は、糖類が低減されながらも十分な濃厚感が付与された、乳入りの加熱殺菌済飲料に関する。 TECHNICAL FIELD The present invention relates to a heat-sterilized milk-containing beverage that has a sufficient richness while reducing sugar content.

乳の風味には、殺菌方法が大きく影響する。加熱殺菌により乳劣化臭が生成されることから、乳劣化臭を抑制する方法が種々提案されている。例えば、乳又は乳製品にα-グリコシルトレハロースを含有させることにより乳加熱臭の生成を抑制する方法(特許文献1)、乳及び乳製品に糖アルコールを0.1~10.0%添加し、乳たんぱく質の遊離スルフヒドルをブロックしてたんぱく質の熱変性を防止する方法(特許文献2)、乳成分と香味成分を含む飲料において、0.0005~0.004質量%のシスチン類を含有させることにより、高温殺菌や加熱保存によって生じる乳成分の劣化臭発生を抑制する方法(特許文献3)等がある。 The flavor of milk is greatly influenced by the sterilization method. Since milk deterioration odor is generated by heat sterilization, various methods have been proposed for suppressing milk deterioration odor. For example, a method of suppressing the production of milk heating odor by containing α-glycosyltrehalose in milk or dairy products (Patent Document 1), adding 0.1 to 10.0% of sugar alcohol to milk and dairy products, A method for preventing thermal denaturation of proteins by blocking free sulfhydrs in milk proteins (Patent Document 2), by incorporating 0.0005 to 0.004% by mass of cystines in a beverage containing milk components and flavor components. , a method for suppressing the generation of deterioration odor of milk components caused by high-temperature sterilization or heat storage (Patent Document 3), and the like.

また、乳入り飲料のコク味や濃厚感を増強する方法も種々提案されている。例えば、フタライド類を有効成分とする飲食品の呈味改善剤を乳飲料を含む様々な飲料に添加する方法(特許文献4)、濃縮牛乳状組成物に甘味料(ブドウ糖とガラクトース)と特定の乳清ミネラルとを含有させる方法(特許文献5)、牛乳と、乳製品と、原料水とを主成分とする乳飲料において、原料水の一部を海洋深層水とする方法(特許文献6)等がある。 Various methods have also been proposed for enhancing the richness and richness of milk-containing beverages. For example, a method of adding a taste improver for foods and drinks containing phthalides as an active ingredient to various beverages including milk drinks (Patent Document 4), a method of adding sweeteners (glucose and galactose) to a concentrated milk-like composition, A method of containing whey minerals (Patent Document 5), and a method of using deep ocean water as part of the raw water in a milk drink whose main components are milk, dairy products, and raw water (Patent Document 6) etc.

特開2006-94856号公報JP2006-94856A 特開平11-46683号公報Japanese Patent Application Publication No. 11-46683 特開2009-55802号公報Japanese Patent Application Publication No. 2009-55802 特開2011-103774号公報Japanese Patent Application Publication No. 2011-103774 特開2011-217645号公報Japanese Patent Application Publication No. 2011-217645 特開2008-67641号公報JP2008-67641A

加熱殺菌工程では、乳の濃厚感が低減することが知られている。具体的には、殺菌乳の乳様香の強度及び濃厚感の強度は、高温短時間殺菌(UHT;120℃、3秒)、高温保持殺菌(HTST;75℃、15秒)、低温保持殺菌(LTLT;63℃、30分)の順で低減する傾向があり、UHTなどの100℃以上の高温加熱を経て製造される乳飲料では、乳のコク味が有意に低減する(「牛乳のおいしさと、その決め手」、雪印メグミルク酪農総合研究所、広報「酪総研」、No.17-4;http://rakusouken.net/topic/017_4_2.html)。特に、糖類が低減された乳入りの飲料では、濃厚感の不足が問題となることが多い。 It is known that the heat sterilization process reduces the richness of milk. Specifically, the intensity of the milky aroma and the intensity of the richness of sterilized milk are determined by high temperature short-time sterilization (UHT; 120°C, 3 seconds), high-temperature holding sterilization (HTST; 75°C, 15 seconds), and low-temperature holding sterilization. (LTLT; 63℃, 30 minutes), and in milk drinks produced through high-temperature heating of 100℃ or higher, such as UHT, the richness of milk decreases significantly (``The richness of milk'' "The Decisive Factor", Megmilk Snow Brand Dairy Research Institute, Public Relations "Dairy Research Institute", No. 17-4; http://rakusouken.net/topic/017_4_2.html). In particular, milk-containing beverages with reduced sugar content often suffer from a lack of richness.

本発明は、糖類が低減された加熱殺菌済みの乳入り飲料において、濃厚感が十分に付与された乳入り飲料を提供することを目的とする。 An object of the present invention is to provide a heat-sterilized milk-containing beverage with reduced sugar content and sufficiently rich texture.

本発明者らは、上記目的を達成すべく鋭意検討した結果、特定量のγ-アミノ酪酸を含有させることにより、加熱殺菌済み乳入り飲料の濃厚感を増強でき、その目的を達成し得ることを見出し、本発明を完成するに至った。 As a result of intensive studies to achieve the above object, the present inventors have found that by incorporating a specific amount of γ-aminobutyric acid, the richness of heat-sterilized milk-containing beverages can be enhanced and the object can be achieved. They discovered this and completed the present invention.

本発明は、これに限定されるものではないが、以下の態様を包含する。
[1]乳成分及び甘味成分を含み、以下成分(A)~(C);
(A)たんぱく質含量 0.5~8.0g/100g、
(B)糖類含量 5.0g/100g以下、及び
(C)γ-アミノ酪酸含量 11~70mg/100g、
を満たす、加熱殺菌済飲料。
[2]甘味成分が、スクラロース、アセスルファムカリウム、及びステビア抽出物より選択される1種以上を含む、[1]に記載の飲料。
[3](D)脂肪含量 0.05~2.0g/100g
をさらに満たす、[1]又は[2]に記載の飲料。
The present invention includes, but is not limited to, the following aspects.
[1] Contains milk components and sweet components, and the following components (A) to (C);
(A) Protein content 0.5-8.0g/100g,
(B) Sugar content 5.0g/100g or less, and (C) γ-aminobutyric acid content 11 to 70mg/100g,
A heat-sterilized beverage that satisfies your needs.
[2] The beverage according to [1], wherein the sweet component contains one or more selected from sucralose, acesulfame potassium, and stevia extract.
[3] (D) Fat content 0.05-2.0g/100g
The beverage according to [1] or [2], which further satisfies the following.

本発明により、糖類含量が5.0g/100g以下に低減されているにも関わらず、十分な濃厚感が付与された加熱殺菌済みの乳入り飲料を提供することが可能となる。 ADVANTAGE OF THE INVENTION According to the present invention, it is possible to provide a heat-sterilized milk-containing beverage that is imparted with a sufficient richness even though the sugar content is reduced to 5.0 g/100 g or less.

本発明の飲料は、十分な濃厚感が付与された加熱殺菌済みの乳入り飲料である。ここで、本明細書でいう濃厚感とは、飲料の呈味が濃厚に感じる感覚であり、飲料を味わった時の全体的な印象が濃厚であると感じるような感覚であり、飲料の良い面、美味しい面での味わいが濃厚になることを意味する。特に、甘味、コク味、旨味など、美味しさに関わっている呈味全体に対して、これらが濃厚になるような感覚である。本発明の十分な濃厚感が付与された乳入り飲料とは、好ましくは、加熱殺菌前の乳入り飲料や、糖類が5.0g/100gより高い飲料と同程度もしくはそれ以上に十分な濃厚感が感じられる飲料であり、飲料を飲み込んだ後もその感覚(特に、乳風味や濃厚感)を楽しむことができる飲料である。 The beverage of the present invention is a heat-sterilized milk-containing beverage that has a sufficiently rich taste. Here, the rich feeling as used in this specification refers to the feeling that the taste of the beverage is rich, the overall impression when tasting the beverage is the feeling that it is rich, and the feeling that the taste of the beverage is rich. It means that the taste of the side and the delicious side will be richer. In particular, it feels like sweetness, richness, umami, and other overall tastes that are related to deliciousness become richer. The milk-containing beverage imparted with a sufficient richness according to the present invention preferably has a richness equivalent to or higher than that of a milk-containing beverage before heat sterilization or a beverage with a sugar content of more than 5.0g/100g. It is a beverage that allows you to enjoy the sensation (particularly the milky flavor and richness) even after swallowing the beverage.

(加熱殺菌済飲料)
本発明でいう「加熱殺菌済飲料」とは、通常の乳入り飲料では濃厚感が低減するような加熱殺菌工程、すなわち100℃以上の高温加熱を経て製造される飲料をいう。そのような高温加熱を用いた加熱殺菌方法としては、特に制限されないが、例えば、高温短時間殺菌(UHT;120~150℃、1~120秒)、レトルト殺菌(110℃~130℃、10~30分)などを挙げることができる。
(heat sterilized beverage)
The term "heat-sterilized beverage" as used in the present invention refers to a beverage manufactured through a heat sterilization process that reduces the richness of ordinary milk-containing beverages, that is, through high-temperature heating of 100° C. or higher. Heat sterilization methods using such high-temperature heating are not particularly limited, but include, for example, high-temperature short-time sterilization (UHT; 120-150°C, 1-120 seconds), retort sterilization (110-130°C, 10-120 seconds), 30 minutes).

(乳入り飲料)
本発明の乳入り飲料は、乳成分及び甘味成分を含む。ここで、本明細書でいう「乳成分」とは、飲料に乳風味や乳感を付与するために添加される牛乳由来の成分を指す。具体的には、生乳、牛乳、特別牛乳、部分脱脂乳、加工乳、クリーム、濃縮乳、無糖れん乳、全粉乳、クリームパウダー、バターミルクパウダー、調整粉乳、脱脂乳、濃縮ホエイ、脱脂濃縮乳、加糖脱脂れん乳、脱脂粉乳、ホエイパウダーなどが挙げられる。複数の乳成分を任意に組み合わせて添加してもよい。中でも、牛乳を含む飲料は本発明の好ましい態様の一つである。
(Drink containing milk)
The milk-containing beverage of the present invention contains a milk component and a sweet component. Here, the term "milk component" as used herein refers to a milk-derived component that is added to impart a milky flavor or feel to a beverage. Specifically, raw milk, milk, special milk, partially skimmed milk, processed milk, cream, concentrated milk, evaporated milk, whole milk powder, cream powder, buttermilk powder, modified milk powder, skimmed milk, concentrated whey, skimmed concentrated milk. Examples include milk, sweetened skim milk, skim milk powder, and whey powder. A plurality of milk components may be added in any combination. Among these, a drink containing milk is one of the preferred embodiments of the present invention.

本発明の乳入り飲料における乳成分の含有量は、たんぱく質(本明細書中、成分(A)とも表記する)を指標として0.5g/100g以上であり、好ましくは0.6g/100g以上であり、より好ましくは0.7g/100g以上である。また、たんぱく質の上限は、8.0g/100g程度である。乳成分の含有量が少ないと通常は加熱殺菌により乳風味が失われる結果、濃厚感が感じられにくくなるが、本発明はそのような乳入り飲料に対しても濃厚感を増強できるという効果がある。すなわち、乳成分の含有量が少ない方が本発明の効果を享受しやすいことから、乳入り飲料中のたんぱく質は5.0g/100g以下がより好ましく、4.0g/100g以下がさらに好ましく、3.0g/100g以下が特に好ましい。たんぱく質含量の測定は、ケルダール法に従って測定を行うことができる。 The content of milk components in the milk-containing beverage of the present invention is 0.5 g/100 g or more, preferably 0.6 g/100 g or more, using protein (also referred to as component (A) in this specification) as an index. Yes, and more preferably 0.7g/100g or more. Moreover, the upper limit of protein is about 8.0g/100g. When the content of milk components is low, the milk flavor is usually lost due to heat sterilization, making it difficult to feel the richness, but the present invention has the effect of enhancing the richness even in such milk-containing drinks. be. That is, since it is easier to enjoy the effects of the present invention when the content of milk components is small, the protein content in the milk-containing beverage is more preferably 5.0 g/100 g or less, even more preferably 4.0 g/100 g or less, and 3. Particularly preferred is .0g/100g or less. The protein content can be measured according to the Kjeldahl method.

また、本明細書でいう「甘味成分」とは、飲料に甘味を付与するために添加される成分を指す。具体的には、黒砂糖、白下糖、カソナード(赤砂糖)、和三盆、ソルガム糖、メープルシュガーなどの含蜜糖、ザラメ糖(白双糖、中双糖、グラニュー糖など)、車糖(上白糖、三温糖など)、加工糖(角砂糖、氷砂糖、粉砂糖、顆粒糖など)、液糖などの精製糖、単糖類(ぶどう糖、果糖、木糖、ソルボース、ガラクトース、異性化糖など)、二糖類(蔗糖、麦芽糖、乳糖、異性化乳糖、パラチノースなど)、オリゴ糖類(フラクトオリゴ糖、マルトオリゴ糖、イソマルトオリゴ糖、ガラクトオリゴ糖、カップリングシュガーなど)、糖アルコール類(エリスリトール、ソルビトール、キシリトール、マンニトール、マルチトール、イソマルチトール、ラクチトール、マルトトリイトール、イソマルトトリイトール、パニトール、オリゴ糖アルコール、粉末還元麦芽糖水飴)などのような糖質甘味料の他、天然非糖質甘味料(ステビア抽出物、カンゾウ抽出物等)や合成非糖質甘味料(アスパルテーム、アセスルファムK等)のような高甘味度甘味料などの甘味料が挙げられる。 Furthermore, the term "sweet ingredient" as used herein refers to an ingredient added to impart sweetness to a beverage. Specifically, brown sugar, white sugar, cassonade (red sugar), wasanbon sugar, sorghum sugar, nectar sugar such as maple sugar, grain sugar (white sugar, medium sugar, granulated sugar, etc.), car sugar (caster sugar, brown sugar, etc.), processed sugar (cube sugar, rock sugar, powdered sugar, granulated sugar, etc.), refined sugar such as liquid sugar, monosaccharides (glucose, fructose, wood sugar, sorbose, galactose, isomerized sugar, etc.) ), disaccharides (sucrose, maltose, lactose, isomerized lactose, palatinose, etc.), oligosaccharides (fructooligosaccharides, maltooligosaccharides, isomaltooligosaccharides, galactooligosaccharides, coupling sugars, etc.), sugar alcohols (erythritol, sorbitol, xylitol) Natural non-carbohydrate sweeteners ( Examples include sweeteners such as high-intensity sweeteners such as Stevia extract, Licorice extract, etc.) and synthetic non-carbohydrate sweeteners (Aspartame, Acesulfame K, etc.).

飲料中における糖類は、甘味の付与に加え固形分として飲料に飲み応えを付与する役割を担っているため、飲料中の糖類含量の低減に伴い、飲料の濃厚感は大きく減少する傾向がある。乳成分及び甘味成分を含む一般的な乳入り飲料の糖類含量(本明細書中、成分(B)とも表記する)は7.0~11.0g/100g程度であるのに対し、本発明の乳入り飲料における糖類含量は、5.0g/100g以下と糖類含量を大きく低減しているため、通常であれば水っぽく薄い味となりやすい。さらに、100℃以上の加熱殺菌を施すことにより、乳入り飲料に求められる乳風味や濃厚感も通常であれば顕著に不足すると考えられる。しかし、本発明では、糖類含量を低減しながらも後述する特定量の成分(C)γ-アミノ酪酸を用いることにより、糖類含量が高い飲料と同程度もしくはそれ以上の濃厚感を備えた飲料を提供することができる。 In addition to imparting sweetness, sugars in beverages play the role of imparting a drinkable feel to the beverage as a solid component, so as the sugar content of the beverage decreases, the richness of the beverage tends to decrease significantly. While the sugar content (also referred to as component (B) in this specification) of general milk-containing beverages containing milk components and sweet components is about 7.0 to 11.0 g/100 g, the sugar content of the present invention Since the sugar content in milk-containing beverages is greatly reduced to 5.0 g/100 g or less, the drink usually tends to have a watery and bland taste. Furthermore, by heat sterilizing at 100° C. or higher, it is thought that the milk flavor and richness normally required for milk-containing beverages will be significantly lacking. However, in the present invention, by using a specific amount of component (C) γ-aminobutyric acid, which will be described later, while reducing the sugar content, a beverage with a richness comparable to or greater than that of beverages with a high sugar content can be created. can be provided.

本発明の効果を享受しやすいという観点から、飲料中の糖類含量(B)は、4.0g/100g以下であることが好ましく、3.0g/100g以下であることがより好ましく、2.5g/100g以下であることがさらに好ましく、2.0g/100g以下であることが特に好ましい。 From the viewpoint of easily enjoying the effects of the present invention, the sugar content (B) in the beverage is preferably 4.0 g/100 g or less, more preferably 3.0 g/100 g or less, and 2.5 g /100g or less is more preferable, and 2.0g/100g or less is particularly preferable.

本明細書において、「糖類含量(B)」は、単糖類及び二糖類の合計量をいうものとする。単糖類には、ぶどう糖、果糖、木糖、ソルボース、ガラクトース、異性化糖などが含まれるが、これらに限定されない。好ましい単糖類は、ぶどう糖、果糖である。また、二糖類とは、2分子の単糖がグリコシド結合した糖類であり、蔗糖、麦芽糖、乳糖、異性化乳糖、パラチノースなどが含まれるが、これらに限定されない。好ましい二糖類は、蔗糖、乳糖である。糖類含量、すなわち単糖類及び二糖類の含有量の測定はHPLCを用いて測定することができる。 In this specification, "saccharide content (B)" refers to the total amount of monosaccharides and disaccharides. Monosaccharides include, but are not limited to, glucose, fructose, wood sugar, sorbose, galactose, isomerized sugar, and the like. Preferred monosaccharides are glucose and fructose. Furthermore, disaccharides are saccharides in which two molecules of monosaccharides are glycosidic bonded, and include, but are not limited to, sucrose, maltose, lactose, isomerized lactose, and palatinose. Preferred disaccharides are sucrose and lactose. The sugar content, that is, the content of monosaccharides and disaccharides, can be measured using HPLC.

飲料の糖類含量を5.0g/100g以下に低減させながら飲料に甘味を与えるためには、糖類(単糖類及び二糖類)に代えて、オリゴ糖類、糖アルコール類、天然非糖質甘味料、高甘味度甘味料を使用する方法が挙げられる。中でも、本発明の効果の顕著さから、スクラロース、アセスルファムカリウム、及びステビア抽出物から選択される1種以上の甘味料を使用するのが好ましい。 In order to sweeten the beverage while reducing the sugar content of the beverage to 5.0g/100g or less, oligosaccharides, sugar alcohols, natural non-carbohydrate sweeteners, A method using a high-intensity sweetener may be mentioned. Among these, it is preferable to use one or more sweeteners selected from sucralose, acesulfame potassium, and stevia extract in view of the remarkable effects of the present invention.

本明細書でいうスクラロースは、4,1’,6’-トリクロロガラクトスクロースを指し、蔗糖の約600倍の甘味を有する高甘味度甘味料である。本発明の飲料におけるスクラロースの含有量は、特に限定されないが、好ましくは0.0008~0.04g/100g、より好ましくは0.001~0.025g/100gである。 Sucralose as used herein refers to 4,1',6'-trichlorogalactosucrose, which is a high-intensity sweetener that is approximately 600 times sweeter than sucrose. The content of sucralose in the beverage of the present invention is not particularly limited, but is preferably 0.0008 to 0.04 g/100 g, more preferably 0.001 to 0.025 g/100 g.

本明細書でいうアセスルファムカリウムは、6-メチル-1,2,3-オキサチアジン-4(3H)-オン-2,2-ジオキシドのカリウム塩を指し、蔗糖の約200倍の甘味を有する高甘味度甘味料である。本発明の飲料におけるアセスルファムカリウムの含有量は、特に限定されないが、好ましくは0.0001~1g/100g、より好ましくは0.001~0.1g/100gである。なお、スクラロースやアセスルファムカリウム等の高甘味度甘味料の濃度は、目的の物質に応じた高速液体クロマトグラフィーを用いた方法で定量できる。例えばアセスルファムカリウムであれば、「高甘味度甘味料 アセスルファムK」(太田静行ら、2002年発行、幸書房)に記載の方法で行うことが可能である。 Acesulfame potassium as used herein refers to the potassium salt of 6-methyl-1,2,3-oxathiazin-4(3H)-one-2,2-dioxide, which has a high sweetness that is approximately 200 times sweeter than sucrose. It is a sweetener. The content of acesulfame potassium in the beverage of the present invention is not particularly limited, but is preferably 0.0001 to 1 g/100 g, more preferably 0.001 to 0.1 g/100 g. Note that the concentration of high-intensity sweeteners such as sucralose and acesulfame potassium can be determined by a method using high-performance liquid chromatography depending on the substance of interest. For example, in the case of acesulfame potassium, it can be carried out by the method described in "High Intensity Sweetener Acesulfame K" (Shizuyuki Ota et al., published in 2002, Saiwai Shobo).

本明細書でいうステビア抽出物は、キク科植物ステビアレバウディアナベルトニー(Stevia rebaudiana BERTOI)(ステビアと略称する)の葉部から抽出されるものであり、蔗糖の約50~500倍の甘味を有する天然甘味料である。ステビア抽出物は、ステビオサイド(Stevioside)、ズルコシドA(Dulcoside-A)、レバウディオサイド(Rebaudioside、以下「Reb」とする。)A、RebB、RebC、RebD、RebF、RebMなどのステビオール配糖体やステビオール等の成分が含まれている。本発明の飲料にステビア抽出物を用いる場合、ステビオール配糖体を80質量%以上含有するものを用いる。本発明の飲料におけるステビア抽出物の含有量は、特に限定されないが、好ましくは0.001~0.5g/100g、より好ましくは0.001~0.25g/100gである。飲料中のステビア抽出物の濃度は、ステビオール配糖体およびα―グルコシルステビオール配糖体の総量を第8版食品添加物公定書(日本食品添加物協会)に記載の方法で定量し、以下の式により算出できる。 The stevia extract referred to herein is extracted from the leaves of the Asteraceae plant Stevia rebaudiana BERTOI (abbreviated as stevia), and has a sweetness that is approximately 50 to 500 times sweeter than sucrose. It is a natural sweetener with Stevia extract contains steviol glycosides such as Stevioside, Dulcoside-A, Rebaudioside (hereinafter referred to as "Reb") A, RebB, RebC, RebD, RebF, and RebM. Contains ingredients such as and steviol. When using a Stevia extract in the beverage of the present invention, one containing 80% by mass or more of steviol glycosides is used. The content of stevia extract in the beverage of the present invention is not particularly limited, but is preferably 0.001 to 0.5 g/100 g, more preferably 0.001 to 0.25 g/100 g. The concentration of Stevia extract in beverages was determined by quantifying the total amount of steviol glycosides and α-glucosyl steviol glycosides using the method described in the 8th edition of the Japan Food Additives Association (Japan Food Additives Association). It can be calculated using the formula.

ステビア抽出物の濃度=(ステビオール配糖体及びα―グルコシルステビオール配糖体の総量)×1.25
(γ-アミノ酪酸)
本発明は、γ-アミノ酪酸(γ-aminobutyric acid, 以下「GABA」と略記する)(本明細書中、成分(C)とも表記する)を添加することにより加熱殺菌済みの乳入り飲料の濃厚感、特に糖類含量が低減された加熱殺菌済みの乳入り飲料の濃厚感を増強する。
Concentration of Stevia extract = (Total amount of steviol glycosides and α-glucosyl steviol glycosides) x 1.25
(γ-aminobutyric acid)
The present invention provides a method for enriching heat-sterilized milk-containing beverages by adding γ-aminobutyric acid (hereinafter abbreviated as "GABA") (also referred to as component (C) herein). To enhance the texture, especially the rich texture of heat-sterilized milk-containing beverages with reduced sugar content.

GABAは、野菜類、果物類、穀類、発酵食品等に幅広く含まれるアミノ酸の一種である。本発明に用いられるGABAとしては、特に限定されるものではなく、例えば野菜類、果物類、穀類などから抽出されたGABA、醗酵によって生産されたGABA、有機合成により得られたGABA等を用いることができる。飲料自体の香味への影響を最小限にして本発明の効果を享受するために、本発明の飲料に用いるGABAとしては、GABAを80%以上、好ましくは85%以上、より好ましくは90%以上含有するGABAの精製品を使用することが好ましい。精製品の形態としては、固体、水溶液、スラリー状など種々のものを用いることができる。市販されているGABAの精製品としては、GABA100%ピュアパウダー(NOW FOODS社)、オリザギャバエキスHC-90(オリザ油化社)などがある。 GABA is a type of amino acid widely contained in vegetables, fruits, grains, fermented foods, and the like. GABA used in the present invention is not particularly limited, and for example, GABA extracted from vegetables, fruits, grains, etc., GABA produced by fermentation, GABA obtained by organic synthesis, etc. can be used. I can do it. In order to enjoy the effects of the present invention while minimizing the influence on the flavor of the beverage itself, the GABA used in the beverage of the present invention should contain 80% or more GABA, preferably 85% or more, and more preferably 90% or more. It is preferable to use a purified product of GABA. Various forms of the purified product can be used, such as solid, aqueous solution, and slurry. Commercially available purified GABA products include GABA 100% Pure Powder (NOW FOODS) and Oryza GABA Extract HC-90 (Oryza Yuka).

本発明においては、飲料中のGABAの濃度が11mg/100g以上、好ましくは15mg/100g以上、さらに好ましくは20mg/100g以上、特に好ましくは25mg/100g以上となるように添加する。GABAの含有量が11mg/100gに満たない場合は、本発明の効果が十分に得られないことがある。また、GABAの含有量は70mg/100g以下が好ましく、65mg/100g以下がより好ましく、60mg/100g以下がさらに好ましい。GABAの含有量は、アミノ酸分析装置を用いて測定することができる。 In the present invention, GABA is added so that the concentration of GABA in the beverage is 11 mg/100 g or more, preferably 15 mg/100 g or more, more preferably 20 mg/100 g or more, particularly preferably 25 mg/100 g or more. If the GABA content is less than 11 mg/100g, the effects of the present invention may not be sufficiently obtained. Moreover, the content of GABA is preferably 70 mg/100 g or less, more preferably 65 mg/100 g or less, and even more preferably 60 mg/100 g or less. The content of GABA can be measured using an amino acid analyzer.

GABAの添加方法は、特に制限されない。上述の加熱殺菌済飲料に対して所定量のGABAを添加してもよいし、加熱殺菌工程前の飲料調合液に予め所定量のGABAを添加しておいてもよい。 The method of adding GABA is not particularly limited. A predetermined amount of GABA may be added to the heat-sterilized beverage described above, or a predetermined amount of GABA may be added in advance to the beverage preparation before the heat-sterilization step.

(その他成分)
本発明の乳入り飲料は、脂肪(本明細書中、成分(D)とも表記する)を含有することが好ましい。脂肪は、乳成分由来の動物性脂肪、又はコーン油、オリーブ油などの植物性脂肪のいずれも使用することができる。飲料中の脂肪含量は、0.05~2.0g/100gが好ましく、0.1~1.0g/100gがより好ましい。脂肪含量の測定は、レーゼ・ゴットリーブ法に従って測定を行うことができる。
(Other ingredients)
The milk-containing beverage of the present invention preferably contains fat (also referred to as component (D) herein). As the fat, either animal fat derived from milk components or vegetable fat such as corn oil or olive oil can be used. The fat content in the beverage is preferably 0.05 to 2.0 g/100 g, more preferably 0.1 to 1.0 g/100 g. The fat content can be measured according to the Loese-Gottlieb method.

常温で長期保存可能な加熱殺菌を行う乳入り飲料では、加熱殺菌時及び保存時の脂肪分離を抑制するために、脂肪球を微細化する均質化処理を行う。生乳の脂肪球の粒子径は約1~10μmであるが、加熱殺菌済飲料では、均質化処理によって、脂肪球の粒子径が約1μm以下程度にまで小さくなる。均質圧が高く、脂肪球の粒子径が小さくなるほど、乳の濃厚感は低減する。したがって、(D)脂肪を含み、均質化処理され、加熱殺菌処理された乳入り飲料は、通常であれば乳の濃厚感が非常に失われやすい飲料である。本発明により、このような飲料に対しても、乳の濃厚感を付与することができるという効果が得られる。均質化処理は、高圧ホモジナイザーなどの均質機を用いて行うことができる。均質化する際の圧力は、特に制限されないが、通常10~50MPa、好ましくは10~40MPa、さらに好ましくは10~30MPa程度である。均質化処理を行う際の温度としては、脂肪(油脂)の凝固点以上の温度を採用することができる。かかる均質化処理温度としては、30~70℃、好ましくは40~70℃の温度を挙げることができる。 Milk-containing beverages subjected to heat sterilization that can be stored for long periods at room temperature are subjected to homogenization treatment to make fat globules finer in order to suppress fat separation during heat sterilization and storage. The particle size of fat globules in raw milk is about 1 to 10 μm, but in heat-sterilized beverages, the particle size of fat globules is reduced to about 1 μm or less by homogenization treatment. The higher the homogeneous pressure and the smaller the particle size of fat globules, the less rich the milk becomes. Therefore, (D) a milk-containing beverage that contains fat, has been homogenized, and has been heat sterilized is a beverage that is normally very likely to lose its milk richness. According to the present invention, it is possible to impart the rich taste of milk to such drinks as well. The homogenization process can be performed using a homogenizer such as a high-pressure homogenizer. The pressure during homogenization is not particularly limited, but is usually about 10 to 50 MPa, preferably about 10 to 40 MPa, and more preferably about 10 to 30 MPa. As the temperature when performing the homogenization treatment, a temperature higher than the freezing point of fat (oil or fat) can be adopted. The temperature for such homogenization treatment may be 30 to 70°C, preferably 40 to 70°C.

その他、本発明の飲料には、本発明の所期の目的を逸脱しない範囲であれば、上記成分に加え、飲料に一般的に配合される成分、例えば、pH調整剤(重曹(炭酸水素ナトリウム)、炭酸カリウム、水酸化カリウム、リン酸三ナトリウム、リン酸三カリウムなど)、酸化防止剤(エリソルビン酸ナトリウムなど)、酸味料、エキス類、香料、着色料、ビタミン、乳化剤、増粘安定剤等を適宜添加することができる。 In addition, the beverage of the present invention may include, in addition to the above-mentioned components, ingredients commonly added to beverages, such as pH adjusters (baking soda (sodium bicarbonate)), as long as they do not depart from the intended purpose of the present invention. ), potassium carbonate, potassium hydroxide, trisodium phosphate, tripotassium phosphate, etc.), antioxidants (sodium erythorbate, etc.), acidulants, extracts, fragrances, colorants, vitamins, emulsifiers, thickening stabilizers etc. can be added as appropriate.

本発明の加熱殺菌済飲料は、通常容器詰めされている。容器の種類は特に限定されない。例えば、PETボトル、缶、瓶、紙容器等が挙げられる。 The heat-sterilized beverage of the present invention is usually packed in containers. The type of container is not particularly limited. Examples include PET bottles, cans, bottles, paper containers, etc.

以下、実験例を示して本発明の詳細を具体的に説明するが、本発明はこれに限定されるものではない。また、本明細書において、特に記載しない限り、数値範囲はその端点を含むものとして記載される。 Hereinafter, the details of the present invention will be specifically explained with reference to experimental examples, but the present invention is not limited thereto. Further, in this specification, unless otherwise specified, numerical ranges are described as including the end points thereof.

実施例の飲料におけるたんぱく質含量の測定はケルダール法に、脂肪含量の測定はレーゼ・ゴットリーブ法に従って、それぞれ測定した。GABA含有量は、測定サンプルを採取して遠心分離した後、その上清を0.02Nの塩酸で処理したものを、0.45μmのフィルターをつけたシリンダを用いてろ過し、これを全自動アミノ酸分析装置(日本電子(株)社製、JLC-500/V)によって定量することにより測定した。また、糖類含量はHPLC糖分析装置(Dionex社製)を用い、検量線法により単糖、二糖をそれぞれ定量して測定し、その合計量を算出した。HPLCの測定条件を以下に示す。
・HPLC装置 : Agilent 1290series
・検出器 : ESA Corona Ultra
・移動相 : A液 水/メタノール=2.5/97.5
B液 アセトニトリル
・グラジェント条件: 0~4min B液 60%、10~11.5min B液 0%
・流速 : 1.2ml/min
・平衡化時間 : 5min
・カラム : 以下のカラムを2本直列で使用。
The protein content and fat content of the beverages of Examples were measured according to the Kjeldahl method and the Loese-Gottlieb method, respectively. GABA content is determined by collecting a measurement sample and centrifuging it, then treating the supernatant with 0.02N hydrochloric acid, filtering it using a cylinder fitted with a 0.45μm filter, and then using a fully automated system. It was measured by quantitative determination using an amino acid analyzer (manufactured by JEOL Ltd., JLC-500/V). In addition, the sugar content was measured by quantifying each monosaccharide and disaccharide by the calibration curve method using an HPLC sugar analyzer (manufactured by Dionex), and the total amount was calculated. The HPLC measurement conditions are shown below.
・HPLC equipment: Agilent 1290series
・Detector: ESA Corona Ultra
・Mobile phase: A liquid water/methanol = 2.5/97.5
B solution Acetonitrile gradient conditions: 0 to 4 min B solution 60%, 10 to 11.5 min B solution 0%
・Flow rate: 1.2ml/min
・Equilibration time: 5min
・Column: Use two of the following columns in series.

上流側 Imtakt Unison UK-Amino HT 3μm 250×3mm
下流側 Imtakt Unison UK-Amino 3μm 250×3mm
・カラム温度 : 65℃
・注入量 : 2μL
実験例1 ミルク入りコーヒー飲料の調製(1)
焙煎度L値20のアラビカ種コーヒー豆を細挽きに粉砕した後、攪拌を行いながら、コーヒー豆の重量の約10倍の重量の90℃の熱水で、15分間抽出を行った。抽出終了後、市販の紙製の濾過フィルターで抽出液を濾過し、濾液を速やかに25℃以下程度まで冷却した。得られた焙煎コーヒー豆抽出液のBrixは2.3であった。このコーヒー豆抽出液と、適量の水と、表1に示す処方の甘味成分(蔗糖、アセスルファムカリウム)及びpH調整剤とを加えて完全に溶解させた後、乳成分(牛乳)、乳化剤、香料を加えて調合液とした(「調合液(未殺菌)」)。この調合液をホモゲナイズ処理(1次圧150kg/cm、2次圧50kg/cm)して均質化した(「調合液のホモゲナイズ処理液(未殺菌)」)。このホモゲナイズ処理液を、90℃に昇温後、190mL缶に充填し、レトルト殺菌(124℃、20分)を行い、容器詰ミルク入りコーヒー飲料(加熱殺菌済み、pH6.3)を製造した。容器詰ミルク入りコーヒー飲料のたんぱく質含量は0.5g/100g、脂肪含量は0.6g/100g、糖類含量は2.6g/100gであった。
Upstream side Imtakt Unison UK-Amino HT 3μm 250×3mm
Downstream side Imtakt Unison UK-Amino 3μm 250×3mm
・Column temperature: 65℃
・Injection volume: 2μL
Experimental example 1 Preparation of coffee drink with milk (1)
After grinding Arabica coffee beans with a roasting degree L value of 20 to a fine grind, extraction was performed for 15 minutes with hot water at 90°C weighing about 10 times the weight of the coffee beans while stirring. After the extraction was completed, the extract was filtered using a commercially available paper filter, and the filtrate was quickly cooled to about 25° C. or lower. The Brix of the obtained roasted coffee bean extract was 2.3. This coffee bean extract, an appropriate amount of water, and the sweetening ingredients (sucrose, acesulfame potassium) and pH adjuster of the formulation shown in Table 1 are added and completely dissolved, and then milk ingredients (milk), emulsifier, and flavoring are added. was added to make a mixed solution ("mixed solution (unsterilized)"). This liquid mixture was homogenized (primary pressure 150 kg/cm 2 , secondary pressure 50 kg/cm 2 ) to make it homogenized (“homogenized liquid mixture (unsterilized)”). This homogenized liquid was heated to 90° C., filled into 190 mL cans, and retort sterilized (124° C., 20 minutes) to produce a packaged milk-containing coffee beverage (heat sterilized, pH 6.3). The protein content of the packaged coffee drink with milk was 0.5 g/100 g, the fat content was 0.6 g/100 g, and the sugar content was 2.6 g/100 g.

Figure 0007417505000001
Figure 0007417505000001

上記の調合液(未殺菌)、調合液のホモゲナイズ処理液(未殺菌)、及び容器詰ミルク入りコーヒー飲料(加熱殺菌済)について、専門パネル5名による官能評価を行った。パネルは、提示されたペアのうちどちらの飲料が濃厚感をより強く感じるか、2点識別試験により評価した。結果を表2に示す。濃厚感は、調合液>ホモゲナイズ処理液>容器詰ミルク入りコーヒー飲料(加熱殺菌済)の順に低下した。これより、容器詰ミルク入りコーヒー飲料の製造における均質化処理工程、加熱殺菌工程の各工程により濃厚感が低減することが示唆された。 The above-mentioned liquid mixture (unsterilized), homogenized liquid of the liquid mixture (unsterilized), and packaged milk-containing coffee beverage (heat-sterilized) were subjected to sensory evaluation by five expert panels. The panel used a two-point discrimination test to evaluate which of the presented pairs of beverages felt more rich. The results are shown in Table 2. The richness decreased in the order of blended liquid > homogenized liquid > packaged milk-containing coffee beverage (heat sterilized). This suggests that the richness is reduced by each step of the homogenization process and heat sterilization process in the production of packaged milk-containing coffee beverages.

Figure 0007417505000002
Figure 0007417505000002

実験例2 ミルク入りコーヒー飲料の調製(2)
GABAを配合すること以外は、実験例1と同様にして容器詰ミルク入りコーヒー飲料を製造した。具体的には、焙煎度L値20のアラビカ種コーヒー豆を細挽きに粉砕した後、攪拌を行いながら、コーヒー豆の重量の約10倍の重量の90℃の熱水で、15分間抽出を行った。抽出終了後、市販の紙製の濾過フィルターで抽出液を濾過し、濾液を速やかに25℃以下程度まで冷却した。このコーヒー抽出液(Brixは2.3)に表3に示すGABA(純度99%以上)を含む各種成分を加えて調合液とした。この調合液をホモゲナイズ処理(1次圧150kg/cm、2次圧50kg/cm)して均質化し、90℃に昇温後、190mL缶に充填し、レトルト殺菌(124℃、20分)を行い、容器詰ミルク入りコーヒー飲料(pH6.3)を製造した。容器詰ミルク入りコーヒー飲料のたんぱく質含量は0.5~0.6g/100g、脂肪含量は0.6g/100g、糖類含量は2.7g/100gであった。
Experimental Example 2 Preparation of coffee drink with milk (2)
A packaged milk-containing coffee beverage was produced in the same manner as in Experimental Example 1, except that GABA was added. Specifically, Arabica coffee beans with a roast level L value of 20 are ground finely, and then extracted for 15 minutes with hot water at 90°C weighing about 10 times the weight of the coffee beans while stirring. I did it. After the extraction was completed, the extract was filtered using a commercially available paper filter, and the filtrate was quickly cooled to about 25° C. or lower. Various components including GABA (purity of 99% or more) shown in Table 3 were added to this coffee extract (Brix: 2.3) to prepare a liquid mixture. This liquid mixture was homogenized by homogenization (primary pressure 150 kg/cm 2 , secondary pressure 50 kg/cm 2 ), heated to 90°C, filled into 190 mL cans, and retort sterilized (124°C, 20 minutes). A packaged milk-containing coffee beverage (pH 6.3) was produced. The protein content of the packaged coffee drink with milk was 0.5-0.6 g/100 g, the fat content was 0.6 g/100 g, and the sugar content was 2.7 g/100 g.

Figure 0007417505000003
Figure 0007417505000003

得られた容器詰ミルク入りコーヒー飲料について、専門パネル5名による官能評価を行った。評価は、GABA無添加の実験例1の容器詰ミルク入りコーヒー飲料(加熱殺菌済み)を対照として、対照と濃厚感の強さに差がないものを「N」(no difference)、対照よりも若干濃厚感が付与された飲料を「A」(a difference)、対照よりも大きく濃厚感が付与され、実験例1の調合液(未殺菌)の濃厚感に近い濃厚感を有するものを「B」(big difference)として、評価したパネルの人数をカウントした。 The resulting packaged milk-containing coffee beverage was subjected to sensory evaluation by five expert panels. The evaluation was made using the packaged milk coffee drink (heat sterilized) of Experimental Example 1 without GABA additives as a control, and the one with no difference in richness compared to the control was ``N'' (no difference). Beverages with a slightly richer texture are labeled as "A" (a difference), and beverages with a richer texture that is greater than the control and have a rich texture similar to that of the unsterilized liquid in Experimental Example 1 are labeled as "B". ” (big difference), and the number of people on the panel who evaluated it was counted.

結果を表4に示す。容器詰ミルク入りコーヒー飲料の場合も、GABA含有量が11~80mg/100gとなるようにGABAを添加することにより、濃厚感が付与されることが示された。特に、飲料中のGABA含有量が25mg/100g以上となるGABAを添加した場合には、パネルの半数以上が「B」(big difference)を選択し、明らかに濃厚感が強くなったと評価し、飲料を飲み込んだ後も乳風味や濃厚感を楽しむことができる飲料であるとコメントした。GABA0~80mg/100gの容器詰ミルク入りコーヒー飲料のうち、最も濃厚感が強いと感じるサンプルをブラインドテストしたところ、GABA含有量:70mg/100gが1人、GABA含有量:80mg/100gが4人であった。一方で、70mg/100gと80mg/100gとでは、パネル全員が濃厚感の強さに大差がないとも評価したことから、経済的観点も加味してGABAの上限は70mg/100g程度が好ましいことがわかった。 The results are shown in Table 4. It has also been shown that in the case of packaged milk-containing coffee beverages, a rich feeling can be imparted by adding GABA so that the GABA content is 11 to 80 mg/100 g. In particular, when GABA was added so that the GABA content in the drink was 25 mg/100 g or more, more than half of the panel selected "B" (big difference), and evaluated that the richness became clearly stronger. They commented that the drink allowed them to enjoy the milky flavor and richness even after swallowing it. When we conducted a blind test on the sample that felt the richest among packaged milk coffee drinks containing GABA 0 to 80 mg/100 g, one person had a GABA content of 70 mg/100 g, and four people had a GABA content of 80 mg/100 g. Met. On the other hand, since all the panelists evaluated that there was not much difference in the strength of the richness between 70mg/100g and 80mg/100g, it is preferable that the upper limit of GABA is around 70mg/100g, taking into account the economic point of view. Understood.

Figure 0007417505000004
Figure 0007417505000004

実験例3 ミルク入りコーヒー飲料の調製(3)
コーヒー豆として、焙煎度L値18のアラビカ種とロブスタ種を半量ずつ混合したブレンド豆を用いた。コーヒー豆を中挽きに粉砕した後、コーヒー豆の重量の約8倍の重量の95℃の熱水でドリップ抽出を行った。抽出終了後、市販の紙製の濾過フィルターで抽出液を濾過し、濾液を速やかに25℃以下程度まで冷却した。得られた焙煎コーヒー豆抽出液のBrixは3.8であった。このコーヒー豆抽出液に、表5に示す処方の各種甘味成分(蔗糖、スクラロース、アセスルファムカリウム、ステビア抽出物;各調合液において甘味度が同等となるように甘味成分の種類に応じて濃度を変更した。)と、乳成分(牛乳)、pH調整剤、乳化剤、香料、及び水を加えて全量1000gとし、よく攪拌して完全に溶解させた。試料3-2、3-4、3-6には、それぞれ試料3-1、3-3、3-5の溶解液に50mg/100gの濃度となるようにγ-アミノ酪酸(GABA)(純度99%以上)を添加して溶解させた。これらの調合液(試料3-1~3-6)を65~70℃に昇温させ、高圧ホモジナイザーにて150kg/cmの圧力で均質化した。このホモゲナイズ処理液を、90℃に昇温後、190mL缶に充填し、レトルト殺菌(122℃、30分)を行い、容器詰ミルク入りコーヒー飲料(加熱殺菌済み)を製造した。殺菌後のpHは6.4であった。なお、ステビア抽出物には、蔗糖の約200倍の甘味を有するステビア抽出物(ベルトロン90、ツルヤ化成工業社製)を用いた。
Experimental example 3 Preparation of coffee drink with milk (3)
The coffee beans used were blended beans made by mixing half Arabica and half Robusta with a roasting degree L value of 18. After grinding the coffee beans to a medium grind, drip extraction was performed using 95° C. hot water with a weight approximately 8 times the weight of the coffee beans. After the extraction was completed, the extract was filtered using a commercially available paper filter, and the filtrate was quickly cooled to about 25° C. or lower. The Brix of the obtained roasted coffee bean extract was 3.8. This coffee bean extract is added to various sweetening ingredients in the formulation shown in Table 5 (sucrose, sucralose, acesulfame potassium, stevia extract; the concentration is changed according to the type of sweetening ingredient so that the sweetness level is the same in each preparation. ), a milk component (milk), a pH adjuster, an emulsifier, a fragrance, and water were added to make a total amount of 1000 g, and the mixture was stirred well to completely dissolve. For samples 3-2, 3-4, and 3-6, γ-aminobutyric acid (GABA) (purity 99% or more) was added and dissolved. These liquid mixtures (samples 3-1 to 3-6) were heated to 65 to 70°C and homogenized using a high-pressure homogenizer at a pressure of 150 kg/cm 2 . This homogenized liquid was heated to 90° C., filled into 190 mL cans, and retort sterilized (122° C., 30 minutes) to produce a packaged milk-containing coffee beverage (heat sterilized). The pH after sterilization was 6.4. As the stevia extract, a stevia extract (Beltron 90, manufactured by Tsuruya Kasei Kogyo Co., Ltd.), which has a sweetness about 200 times that of sucrose, was used.

Figure 0007417505000005
Figure 0007417505000005

得られた容器詰ミルク入りコーヒー飲料(加熱殺菌済み)について、たんぱく質、脂肪、及び糖類含量を分析した。また、専門パネル5名による官能評価を行った。評価は、GABA無添加の容器詰ミルク入りコーヒー飲料(加熱殺菌済み)(試料3-1、3-3、3-5)を対照として、試料3-2、3-4、3-6のそれぞれについて対照と濃厚感の強さに差がないものを「N」(no difference)、対照よりも若干濃厚感が付与された飲料を「A」(a difference)、対照よりも大きく濃厚感が付与された飲料を「B」(big difference)として、評価したパネルの人数をカウントした。 The protein, fat, and sugar content of the obtained packaged milk-containing coffee beverage (heat sterilized) was analyzed. In addition, a sensory evaluation was conducted by five expert panelists. The evaluation was conducted using GABA-free packaged milk coffee drinks (heat sterilized) (Samples 3-1, 3-3, 3-5) as controls, and Samples 3-2, 3-4, and 3-6, respectively. "N" (no difference) indicates that there is no difference in the strength of the richness compared to the control; "A" (a difference) indicates that the drink has a slightly richer feel than the control; The number of people on the panel who evaluated the beverage was counted as "B" (big difference).

表6に成分分析の結果を、表7に官能評価結果を示す。糖類含量が7.7g/100gである飲料ではGABA添加による濃厚感付与の効果がほとんど感じられないが、糖類含量が0.7g/100gである飲料ではGABA添加による濃厚感が顕著に付与され、試料3-4や試料3-6の濃厚感は、試料3-2と同程度の濃厚感であると評価された。 Table 6 shows the results of component analysis, and Table 7 shows the results of sensory evaluation. In beverages with a sugar content of 7.7g/100g, the effect of adding GABA to give a rich feeling is hardly felt, but in drinks with a sugar content of 0.7g/100g, the addition of GABA significantly imparts a rich feeling. The richness of samples 3-4 and 3-6 was evaluated to be similar to that of sample 3-2.

Figure 0007417505000006
Figure 0007417505000006

Figure 0007417505000007
Figure 0007417505000007

実験例4 ミルク入りコーヒー飲料の調製(4)
実験例3のスクラロースとアセスルファムカリウムを配合した飲料(試料3-3)について、乳成分の牛乳を表8に示す全粉乳及び脱脂粉乳に変える以外は、実験例3と同様にして、容器詰ミルク入りコーヒー飲料(加熱殺菌済み)を製造した。試料4-2、4-4、4-6、4-8、4-10、4-12には、50mg/100gの濃度となるようにγ-アミノ酪酸(GABA)(純度99%以上)を添加して溶解させた。各飲料について、成分分析を行った。また、実験例3と同様にして専門パネル5名による官能評価を行った。
Experimental Example 4 Preparation of coffee drink with milk (4)
Concerning the beverage containing sucralose and acesulfame potassium (Sample 3-3) of Experimental Example 3, packaged milk was prepared in the same manner as in Experimental Example 3, except that the milk component was replaced with whole milk powder and skim milk powder shown in Table 8. A coffee drink (heat sterilized) was produced. Samples 4-2, 4-4, 4-6, 4-8, 4-10, and 4-12 were treated with γ-aminobutyric acid (GABA) (purity of 99% or more) at a concentration of 50 mg/100 g. Add and dissolve. Component analysis was conducted for each beverage. Further, in the same manner as in Experimental Example 3, sensory evaluation was performed by five expert panels.

表9に成分分析の結果を、表10に官能評価結果を示す。糖類含量が低い飲料では、GABA添加により乳風味や濃厚感が付与された。特に、試料4-1~4-4の結果から、飲料中の脂肪含量が0.05g/100g以上であると、より一層本発明の効果を確認しやすいことが示された。また、飲料中のたんぱく質含量が0.4g/100gの試料4-7及び4-8では、GABA添加による濃厚感付与の効果が顕著ではなかったことから、本発明の効果を享受するためには、たんぱく質含量は0.5g/100g以上であることが示された。 Table 9 shows the results of component analysis, and Table 10 shows the results of sensory evaluation. In beverages with low sugar content, the addition of GABA imparted milky flavor and richness. In particular, the results of Samples 4-1 to 4-4 showed that it was easier to confirm the effects of the present invention when the fat content in the beverage was 0.05 g/100 g or more. In addition, in Samples 4-7 and 4-8 where the protein content in the drink was 0.4g/100g, the effect of adding GABA to give a rich feeling was not significant, so in order to enjoy the effects of the present invention, It was shown that the protein content was 0.5g/100g or more.

Figure 0007417505000008
Figure 0007417505000008

Figure 0007417505000009
Figure 0007417505000009

Figure 0007417505000010
Figure 0007417505000010

実験例5 ミルク入りコーヒー飲料の調製(5)
実験例3のスクラロースとアセスルファムカリウムを配合した飲料(試料3-3)、アセスルファムカリウムとステビア抽出物を配合した飲料(試料3-5)について、GABAの配合量を表11の処方に変える以外は、同様の方法で調合液を得、ホモジナイズ処理液を調製した。このホモゲナイズ処理液を、141℃、30秒にてプレート殺菌を行い、PET容器に充填し、容器詰ミルク入りコーヒー飲料(加熱殺菌済み)を製造した。殺菌後のpHは6.4であった。
Experimental Example 5 Preparation of coffee drink with milk (5)
Regarding the drink containing sucralose and acesulfame potassium (sample 3-3) and the drink containing acesulfame potassium and stevia extract (sample 3-5) of Experimental Example 3, except for changing the amount of GABA to the formulation shown in Table 11. A mixed solution was obtained in the same manner as above, and a homogenized solution was prepared. This homogenized solution was plate sterilized at 141° C. for 30 seconds and filled into a PET container to produce a packaged milk-containing coffee beverage (heat sterilized). The pH after sterilization was 6.4.

得られた容器詰ミルク入りコーヒー飲料(加熱殺菌済み)について、実験例3と同様にして専門パネル5名による官能評価を行った。結果を表11に示す。PET入りの容器詰ミルク入りコーヒー飲料においても、11~70mg/100gとなるようにGABAを添加することにより、濃厚感が付与されることが示された。 The obtained packaged milk-containing coffee beverage (heat sterilized) was subjected to sensory evaluation by five expert panelists in the same manner as in Experimental Example 3. The results are shown in Table 11. It has been shown that even in a coffee drink containing milk packaged in a PET container, a rich feeling can be imparted by adding GABA to a concentration of 11 to 70 mg/100 g.

Figure 0007417505000011
Figure 0007417505000011

実験例6 ミルク入り紅茶飲料の調製
紅茶葉20gを600gの熱水(90℃)で、5分間抽出を行った。抽出終了後、メッシュで固液分離し、濾液を速やかに25℃以下まで冷却した後、遠心分離処理して紅茶抽出液を得た。この紅茶抽出液に表12に示す甘味成分(蔗糖、アセスルファムカリウム)、乳成分(牛乳、全粉乳、脱脂粉乳)、その他各種成分を加えて全量を1000gとし、高圧ホモジナイザーにて150kg/cmの圧力で均質化した。このホモゲナイズ処理液を、141℃、30秒にてプレート殺菌を行い、PET容器に充填し、容器詰ミルク入り紅茶飲料(加熱殺菌済み)を製造した。殺菌後のpHは6.4であった。容器詰ミルク入り紅茶飲料のたんぱく質含量は0.9~1.0g/100g、脂肪含量は0.5g/100g、糖類含量は3.3g/100gであった。
Experimental Example 6 Preparation of black tea beverage with milk 20 g of black tea leaves were extracted with 600 g of hot water (90° C.) for 5 minutes. After the extraction was completed, the solid and liquid were separated using a mesh, and the filtrate was quickly cooled down to 25° C. or lower, followed by centrifugation to obtain a black tea extract. Sweet ingredients (sucrose, acesulfame potassium), milk ingredients (milk, whole milk powder, skim milk powder), and other various ingredients shown in Table 12 were added to this black tea extract to make a total amount of 1000 g, and the mixture was heated to 150 kg/cm 2 using a high-pressure homogenizer. Homogenized under pressure. This homogenized solution was plate sterilized at 141° C. for 30 seconds and filled into a PET container to produce a packaged black tea beverage containing milk (heat sterilized). The pH after sterilization was 6.4. The protein content of the packaged black tea beverage with milk was 0.9-1.0 g/100 g, the fat content was 0.5 g/100 g, and the sugar content was 3.3 g/100 g.

得られた容器詰ミルク入り紅茶飲料(加熱殺菌済み)について、実験例3と同様にして専門パネル5名による官能評価を行った。結果を表13に示す。容器詰ミルク入り紅茶飲料においても、11~70mg/100gとなるようにGABAを添加することにより、乳の濃厚感が付与されることが示された。 The resulting packaged black tea beverage containing milk (heat sterilized) was subjected to sensory evaluation by five expert panelists in the same manner as in Experimental Example 3. The results are shown in Table 13. It has been shown that even in packaged tea drinks containing milk, the richness of milk can be imparted by adding GABA to a concentration of 11 to 70 mg/100 g.

Figure 0007417505000012
Figure 0007417505000012

Figure 0007417505000013
Figure 0007417505000013

実験例7 ミルク飲料の調製
表14に示す甘味成分(アセスルファムカリウム、スクラロース)、乳成分(全粉乳、脱脂粉乳、乳清たんぱく)、その他各種成分を加えて全量を1000gとし、よく攪拌して完全に溶解させ調合液とした。この調合液を65~70℃に昇温させ、高圧ホモジナイザーにて150kg/cmの圧力で均質化した。このホモゲナイズ処理液を、90℃に昇温後、190mL缶に充填し、レトルト殺菌(122℃、30分)を行い、容器詰ミルク飲料(加熱殺菌済み)を製造した。殺菌後のpHは6.5であった。なお、乳清たんぱくには、ホエイたんぱく濃縮物(WPC/80、Nutra Food Ingredients, LLC)を用いた。容器詰ミルク飲料のたんぱく質含量は7.5g/100g、脂肪含量は0.06g/100g、糖類含量は1.4g/100gであった。
Experimental Example 7 Preparation of Milk Beverage Add sweet ingredients (acesulfame potassium, sucralose), milk ingredients (whole milk powder, skim milk powder, whey protein), and other various ingredients shown in Table 14 to make a total amount of 1000 g, and stir well to completely dissolve. It was dissolved in to prepare a liquid preparation. This liquid mixture was heated to 65 to 70°C and homogenized using a high-pressure homogenizer at a pressure of 150 kg/cm 2 . This homogenized liquid was heated to 90° C., filled into 190 mL cans, and retort sterilized (122° C., 30 minutes) to produce a packaged milk beverage (heat sterilized). The pH after sterilization was 6.5. Note that whey protein concentrate (WPC/80, Nutra Food Ingredients, LLC) was used as the whey protein. The protein content of the packaged milk drink was 7.5 g/100 g, the fat content was 0.06 g/100 g, and the sugar content was 1.4 g/100 g.

得られた容器詰ミルク飲料(加熱殺菌済み)について、実験例3と同様にして専門パネル5名による官能評価を行った。結果を表15に示す。容器詰ミルク飲料においても、11~70mg/100gとなるようにGABAを添加することにより、乳の濃厚感が付与されることが示された。 The obtained packaged milk beverage (heat sterilized) was subjected to sensory evaluation by five expert panelists in the same manner as in Experimental Example 3. The results are shown in Table 15. Even in packaged milk drinks, it has been shown that adding GABA to a concentration of 11 to 70 mg/100g imparts a rich milk feel.

Figure 0007417505000014
Figure 0007417505000014

Figure 0007417505000015
Figure 0007417505000015

Claims (3)

乳成分及び甘味成分を含み、以下成分(A)~(C);
(A)たんぱく質含量 0.5~8.0g/100g、
(B)糖類含量 5.0g/100g以下、及び
(C)γ-アミノ酪酸含量 25~70mg/100g、
を満たす、加熱殺菌済飲料。
Contains milk components and sweet components, and the following components (A) to (C);
(A) Protein content 0.5-8.0g/100g,
(B) Sugar content 5.0g/100g or less, and (C) γ-aminobutyric acid content 25 to 70mg/100g,
A heat-sterilized beverage that satisfies your needs.
甘味成分が、スクラロース、アセスルファムカリウム、及びステビア抽出物より選択される1種以上を含む、請求項1に記載の飲料。 The beverage according to claim 1, wherein the sweet component includes one or more selected from sucralose, acesulfame potassium, and stevia extract. (D)脂肪含量 0.05~2.0g/100g
をさらに満たす、請求項1又は2に記載の飲料。
(D) Fat content 0.05-2.0g/100g
The beverage according to claim 1 or 2, further satisfying the following.
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