WO2015186956A1 - 반추동물의 메탄 생성 저감용 사료 첨가제 조성물 - Google Patents
반추동물의 메탄 생성 저감용 사료 첨가제 조성물 Download PDFInfo
- Publication number
- WO2015186956A1 WO2015186956A1 PCT/KR2015/005541 KR2015005541W WO2015186956A1 WO 2015186956 A1 WO2015186956 A1 WO 2015186956A1 KR 2015005541 W KR2015005541 W KR 2015005541W WO 2015186956 A1 WO2015186956 A1 WO 2015186956A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- methane
- nitrate
- berberine
- composition
- feed
- Prior art date
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/137—Heterocyclic compounds containing two hetero atoms, of which at least one is nitrogen
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/22—Methane [CH4], e.g. from rice paddies
Definitions
- the present invention relates to a feed additive composition for reducing methane production in ruminants of ruminants, and in particular, methane production comprising one or more components selected from the group consisting of Alliin and Berberine.
- the present invention relates to a feed additive composition for abatement, wherein the composition may further include one or more components selected from the group consisting of diallyl disulfide (DADS), nitrate, and eucalyptus oil. .
- DADS diallyl disulfide
- the amount of greenhouse gas produced by maintaining the livestock industry is estimated to be about 4.1 billion to 7.1 billion tons per year, which is equivalent to carbon dioxide, which is about 15 to 24% of the total greenhouse gas production (Steinfeld et al. , 2006).
- the amount of carbon dioxide, methane, and nitrous oxide produced by livestock accounts for 9%, 35-40%, and 65% of human activities, which is a serious level.
- the addition of antibiotics to feed is a global trend to increasingly regulate the use of chemicals and antibiotics as feed additives, and remains a problem that may be harmful to the human body.
- Removing the protozoa has a problem that can reduce the fiber, and adding a halogen compound to the feed does not sustain the inhibitory effect of methane gas and may cause safety problems such as residual to livestock products.
- the method of feeding the probiotic has a problem in that the strain added to the fermentation pattern and the methane gas production experiment of the probiotic is different.
- Korean Patent Publication No. 10-2006-0019062 discloses a feed composition that suppresses the methane production of ruminants.
- the patent is characterized in that the feed composition comprising at least one of ginger, leek extract and complex linoleic acid to the ruminant to reduce the amount of methane gas produced in the ruminant.
- the feed composition comprising at least one of ginger, leek extract and complex linoleic acid to the ruminant to reduce the amount of methane gas produced in the ruminant.
- the disclosed ginger, leek extract and complex linoleic acid requires a separate process, there is a problem that can not be easily obtained from the surroundings, the manufacturing cost is required a lot, and accordingly the purchase cost There is a problem that increases.
- ruminant models in which diallyl sulfide, allin, nitrate, berberine and eucalyptus oils or mixtures thereof are developed under the same conditions as in the rumen environment.
- the present invention was completed by confirming that the result of incubation with a rumen simulation continuous culture system (RSCC) showed a significant methane reduction rate compared to the control.
- RSCC rumen simulation continuous culture system
- One object of the present invention is to provide a feed additive composition for reducing methane production having a safe and efficient methane production reducing effect comprising at least one of diallyl sulfide, alline, nitrate, berberine and eucalyptus oil.
- Another object of the present invention is to provide a method of reducing methane production comprising administering the feed additive composition to a subject.
- the feed additive composition according to the present invention can be very useful in the ruminant industry because it can reduce the amount of methane generated in the rumen without negatively affecting the productivity of the livestock by adding to the feed without additional treatment process.
- DADS diallyl sulfide
- Figure 2 is a graph showing the methane reduction rate according to the addition of Allin (Alliin) according to an embodiment of the present invention.
- FIG 3 is a graph showing the methane reduction rate according to the nitrate (Nitrate) addition content according to an embodiment of the present invention.
- Figure 4 is a graph showing the methane reduction rate according to the addition of eucalyptus oil (Eucalyptus oil) according to an embodiment of the present invention.
- Figure 5 is a graph showing the methane reduction rate according to the content of berberine (Berberine) according to an embodiment of the present invention.
- DADS diallyl sulfide
- Nirate nitrate
- Eucalyptus oil eucalyptus oil
- berberine mixed composition according to an embodiment of the present invention.
- FIG. 7 is a graph showing the methane reduction rate according to the content of alliin, nitrate, eucalyptus oil and berberine mixed composition according to an embodiment of the present invention.
- DADS diallyl sulfide
- Naitrate nitrate
- Eucalyptus oil eucalyptus oil
- berberine mixed composition according to an embodiment of the present invention to be.
- one embodiment of the present invention provides a feed additive composition for reducing methane production comprising at least one component selected from the group consisting of Alliin and Berberine.
- composition may be added to the feed in an amount such that allin is added to 0.005 wt% to 5 wt% or berberine to 0.005 wt% to 5 wt% relative to the dry feed weight.
- Alliin refers to a garlic or onion odor component such as garlic, onion, and the like (2R) -2-amino-3-[(S) -prop-2-enylsulfinyl] propanoic acid ((2R) -2-amino-3-[(S) -prop-2-enylsulfinyl] propanoic acid).
- the feed additive composition may include 1 to 100% by weight, specifically 10 to 90% by weight, more specifically 20 to 80% by weight, but is not limited thereto.
- berine refers to quaternary ammonium salt belonging to the Protoberberine group of isoquinoline alkaloid, and 5,6-dihydro-9, 10-dimethoxybenzo [g] -1,3-benzodiozolo [5,6-a] quinolinium (5,6-dihydro-9,10-dimethoxybenzo [g] -1,3-benzodioxolo [5, 6-a] quinolizinium).
- the feed additive composition may include 1 to 100% by weight of berberine, specifically 10 to 90% by weight, more specifically 20 to 80% by weight, but is not limited thereto.
- methane refers to methane gas (CH 4 ), which is known to be the main culprit of global warming as it occurs in the decomposition of various organic substances.
- carbohydrates turn into volatile fatty acids and some become methane gas during the fermentation of food by various microorganisms in the rumen, and this methane gas accounts for about one fourth of the global methane gas emissions.
- Ruminant methane production in the rumen results in about 10% loss of feed energy consumed. In other words, the generation of fermentation gas containing methane produced in the rumen not only contributes to global warming, but also may lower the energy absorption efficiency of livestock and thus affect productivity.
- the methane reduction rate is 21.9% lower than diallyl sulfide
- the pH remained similar to the control.
- allicin had a lower methane reduction effect than diallyl sulfide, it appeared to be more effective in stabilizing the rumen fluid.
- the methane reduction rate is 6.7 %, 12.4%, 45.5%.
- the methane reduction effect was high as 45.5%, but the amount of less than 0.5wt% was shown to be an appropriate level because the dry digestion rate was significantly decreased.
- composition may further include one or more components selected from the group consisting of diallyl disulfide (DADS), nitrate (Nitrate) and eucalyptus oil (Eucalyptus oil).
- DADS diallyl disulfide
- Nitrate nitrate
- Eucalyptus oil eucalyptus oil
- DADS Diaallyl disulfide
- the term "Diallyl disulfide (DADS)" of the present invention refers to 4,5-dithia-1,7-octadiene diallyl sulfide (4,5-dithia-1,7-octadiene Diallyldisulfide) It is known to be effective in improving blood circulation, antibacterial and blood circulation. It is known to be effective in preventing adult diseases such as hypertension, arteriosclerosis, angina pectoris, myocardial infarction and stroke.
- the feed additive composition may include 1 to 90% by weight of diallyl sulfide, specifically 20 to 80% by weight, but is not limited thereto.
- nitrate (Nitrate) is nitrate ions NO 3 - as a general term for the compounds, such as sodium nitrate (sodium nitrate), nitric acid potassium (potassium nitrate), nitric acid calcium (calcium nitrate), ammonium nitrate ( ammonium nitrate) and the like, but is not limited thereto.
- the feed additive composition may include 1 to 90% by weight of nitrate, specifically 20 to 80% by weight, but is not limited thereto.
- Eucalyptus oil refers to essential oils obtained by distilling the leaves of Eucalyptus trees. It is a colorless to pale yellow transparent liquid with a characteristic stinging aroma. The taste is irritating or refreshing.
- the feed additive composition may include 1 to 90% by weight of eucalyptus oil, specifically 20 to 80% by weight, but is not limited thereto.
- the composition is 0.005 wt% to 4 wt% of diallyl sulfide based on the weight of dry feed, 0.005 wt% to 5 wt% of allin, 0.01 wt% to 0.5 wt% of nitrate, 0.005 wt% to 5 wt of berberine % Or eucalyptus oil may be added to the feed in an amount such that it is added at 0.01 wt% to 5 wt%, but is not limited thereto. If more than 0.5 wt% of nitrate is added, problems such as nitrite accumulation and nitrate toxicity syndrome may occur.
- the methane reduction rate is 30.5%, 65.2% and 65.9%.
- the reduction rate of methane according to the addition content of 0.2 wt% and 0.4 wt% was about 65%, which means that adding 0.2 wt% diallyl sulfide may be economically advantageous. it means.
- the methane reduction rate was 14.0. %, 31.0%, 8.1%.
- the methane reduction rate when the eucalyptus oil was added in an amount of 0.5 wt% per 10 g of the substrate using the RSCC system, the methane reduction rate was 20.5%. Methane reduction was lower than that of diallyl sulfide at the same addition level, but more effective in stabilizing the rumen fluid.
- Feed additive composition of the present invention may include a mixture of each of the above active ingredients, in order to minimize side effects.
- the mixed composition has a synergistic effect, it can exhibit an effective methane reduction effect with a small amount of addition.
- the composition may comprise diallyl sulfide, nitrate, berberine and eucalyptus oil.
- the content of diallyl sulfide, nitrate, berberine and eucalyptus oil is as described above.
- the composition is 0.005 wt% to 4 wt% of diallyl sulfide, 0.01 wt% to 0.5 wt% of nitrate, 0.005 wt% to 5 wt% of berberine, and 0.01 wt% to eucalyptus oil, based on the dry feed weight. It may be added to the feed in an amount such that it is added at 5 wt%.
- an effective methane reduction rate of 56.2% is obtained when 0.05 wt% of diallyl sulfide, 0.2 wt% of nitrate, 0.05 wt% of berberine and 0.2 wt% of eucalyptus oil are used as an additive. It was confirmed that there is an effect of showing a good reduction rate in a small amount even when using a different mixed composition as an additive.
- the effective rate of methane reduction was 23.1% for feeding and 36.6% for 50g / d.
- the flow rate also increased by more than 1.1% without decreasing compared to the control, it was confirmed that there is no negative effect on the fermentation properties of the methane reduction mixed composition.
- the composition may include allin, nitrate, berberine and eucalyptus oil.
- the content of the allicin, nitrate, berberine and eucalyptus oil is as described above.
- the composition is 0.005 wt% to 5 wt% of allin, 0.01 wt% to 0.5 wt% of nitrate, 0.005 wt% to 5 wt% of berberine, and 0.01 wt% to 5 wt of eucalyptus oil. It may be added to the feed in an amount such that it is added in%.
- the weight of the dry feed weight of allelin, berberine each 0.05 wt% of the addition of the content of nitrate and eucalyptus oil was measured by varying the 0.1 wt% or 0.2 wt%, as a result, each additive is added alone Methane reduction rate was increased than when the synergistic effect was confirmed.
- the individual to which the feed additive composition for reducing methane can be applied is not particularly limited, and any form may be applied.
- any form may be applied.
- animals such as cows, sheep, giraffes, camels, deer, goats, and the like, and specifically to ruminants having rumens.
- ruminant is a special digestive tract found in some animals of mammalian joiners, and is divided into four rooms, called hump, honeycomb, folds, and wrinkles, for the purpose of rubbing. Also known as ruminwiwi, once swallowed food into the mouth again to chew well swallow swallowing, this rumen is called the stomach to enable rumination. In the rumen, microbial symbiosis has the ability to decompose and energize the cellulose of plants that cannot be digested by ordinary animals.
- ruminant refers to an animal having the rumen described above, and includes the animals of the family Camel, Deer, Deer, Giraffe and Bovine. However, the camel family and the baby deer are known to have a rumen consisting of three rooms because the folds and wrinkles are not completely differentiated.
- the feed additive composition according to the present invention may be used individually, may be used in combination with a conventionally known feed additive, and may be used sequentially or simultaneously with a conventional feed additive.
- the feed additive composition according to the present invention includes not only the physiologically acceptable salts of allin, berberine or diallyl sulfide, but also all possible solvates and hydrates that may be prepared therefrom, and may include all possible stereoisomers.
- the solvates, hydrates and stereoisomers of the allin, berberine or diallyl sulfide can be prepared using conventional methods.
- physiologically acceptable is physiologically acceptable and when administered to an organism, the compound to be administered can exert its desired effect, usually without causing an allergic or similar reaction, such as gastrointestinal disorders, dizziness, and the like. Means commonly used.
- allin, berberine and diallyl sulfide may be obtained through natural products, chemical synthesis or microbial fermentation, and may be purchased and used commercially.
- One aspect of the invention provides a method of reducing methane production comprising administering said feed additive composition to a subject.
- 250 ml of distilled water was added to a 2 L Erlenmeyer flask, 0.12 ml of a micromineral solution was added and stirred, and 250 ml of distilled water was added thereto and stirred for 20 minutes.
- 250 ml of in vitro buffer solution was added and stirred for 10 minutes, followed by 250 ml of bulk mineral solution, followed by stirring for 15 minutes. Thereafter, 1.25 ml of a resazurin solution was added and stirred.
- the inlet of the Erlenmeyer flask was blocked with aluminum foil and heated while bubbling anaerobic gas with carbon dioxide gas in a Hergate anaerobic device. When the solution started to boil, it was heated for about 10 minutes more, stopped and cooled. To the cooled solution was added 50 ml of reduction solution to obtain a buffer. The completed buffer was found to change color of the solution to colorless.
- the collected gastric juice was filtered with 8 layers of gauze and filtered with glass wool.
- the filtered gastric juice was bubbling with carbon dioxide gas.
- the buffer prepared in 1) above was added while maintaining an anaerobic state with carbon dioxide gas, and mixed with gastric juice (250 ml of gastric juice and 600 ml of buffer).
- the buffer prepared in 1) was put into the buffer container while maintaining the anaerobic state.
- the Tedlar bag filled with carbon dioxide gas was connected to the buffer container and the tube connected to the buffer container was connected to the buffer inlet of the fermenter lid.
- the tube connected to the gas collection bag was connected to the gas collecting port of the fermenter lid (the connecting tube was a gas tube).
- the pH and temperature electrodes were put in such a way that the electrodes were sufficiently in contact with the culture solution while being careful not to touch the stirring rod, and the feed tube was inserted into the feed inlet of the fermenter lid so as to be sufficiently immersed in the culture solution.
- the lid of the fermenter was connected to the fermenter and kept anaerobic using vacuum grease and Teflon tape. Carbon dioxide gas was introduced into the fermenter for about 10 minutes through the gas collecting port of the fermenter lid (the inside of the fermenter was changed to anaerobic state, and carbon dioxide gas was continuously flowed until the incubation started). Sealed with Teflon tape. After all the devices were connected, the peristaltic pump was operated and a buffer was introduced into the fermenter. The anaerobic broth was introduced into the fermenter until it overflowed through the feed inlet, and then the CO2 gas was stopped and all inlets were blocked.
- the circulating water tank and the fermentation tank were connected, set to a temperature of 39 ° C., and the culture was started with stirring at 99 rpm to 102 rpm with a stirring rod.
- the culture medium of the fermenter was kept constant at pH 6.8 ⁇ 7.1, temperature 39 °C -40 °C, and maintained a turnover rate of 0.042 h -1 .
- the turnover rate of the substrate was maintained at 0.017 h ⁇ 1 .
- the methane generation reduction effect experiment was carried out according to the operation method described above using the rumen model continuous culture system (RSCC system) of Preparation Example 3, the feed additive for methane reduction was added with the substrate at the time of substrate addition. .
- RSCC system rumen model continuous culture system
- the rumen model continuous culture system was operated during the adaptation period (5 days), and then the total gas generation, methane generation and pH values of the control were measured for 3 days.
- diallyl disulfide (DADS) is added at a ratio of 0.1 wt%, 0.2 wt% and 0.4 wt% per 10 g of substrate, and the total amount of gas generated at the same time every day for 3 days as in the control.
- Methane generation amount and pH value were measured.
- the methane reduction rate was measured after the adaptation period with the newly collected rumen liquid, and the measurement results are shown in Table 1 and FIG. 1.
- the pH of the rumen solution showed a tendency to decrease as the diallyl sulfide addition content was increased, the methane generation was reduced by 30.5%, 65.2% and 65.9% compared to the control.
- the reduction rate of methane according to the addition content of 0.2 wt% and 0.4 wt% was about 65%, which means that adding 0.2 wt% diallyl sulfide may be economically advantageous. it means.
- the rumen model continuous culture system was operated during the adaptation period (5 days), and then the total gas generation, methane generation and pH values of the control were measured for 3 days. After the control measurement was completed, Alliin was added at a rate of 0.5 wt% per 10 g of substrate, and the total gas generation amount, methane generation amount, and pH value were measured at the same time every day for three days as in the control. Each time the addition content was verified, the methane reduction rate was measured after the adaptation period with freshly collected rumen liquid, and the measurement results are shown in Table 2 and FIG. 2.
- the rumen model continuous culture system was operated during the adaptation period (5 days), and then the total gas generation, methane generation and pH values of the control were measured for 3 days. After the control measurement was completed, nitrate was added at a rate of 0.35 wt%, 0.5 wt% and 1.0 wt% per 10 g of substrate, and the total gas generation, methane generation and pH values were measured at the same time every day for 3 days as in the control. It was. Every time the addition content was verified, the methane reduction rate was measured after a period of adaptation with freshly collected rumen liquid, and the measurement results are shown in Table 3 and FIG. 3.
- the rumen model continuous culture system was operated during the adaptation period (5 days), and then the total gas generation, methane generation and pH values of the control were measured for 3 days. After the control measurement was completed, Eucalyptus oil was added at a rate of 0.5 wt% per 10 g of substrate, and the total gas generation, methane generation and pH values were measured at the same time every day for 3 days as in the control. The measurement results are shown in Table 4 and FIG. 4.
- the rumen model continuous culture system was operated during the adaptation period (5 days), and then the total gas generation, methane generation and pH values of the control were measured for 3 days.
- berberine is added at a ratio of 0.1 wt%, 0.2 wt% and 0.5 wt% per 10 g of substrate, and the total gas generation, methane generation, pH at the same time every day for 3 days as in the control.
- the numerical value was measured.
- the methane reduction rate was measured after the adaptation period with freshly collected rumen liquid, and the measurement results are shown in Table 5 and FIG. 5.
- diallyl sulfide, nitrate, berberine and eucalyptus oil blending additives are added at a ratio of 0.3 wt% or 0.5 wt% to 10 g of substrate, and the total amount of gas generated at the same time every day for 3 days, Methane generation amount and pH value were measured. Each time the addition content was verified, the methane reduction rate was measured after a period of adaptation with freshly collected rumen liquid, and the measurement results are shown in Table 6 and FIG. 6.
- the methane reduction rate was significantly increased as compared to when the additives were added alone, as the dialyl sulfide, nitrate, berberine and eucalyptus oil were mixed to confirm the methane reduction rate.
- Diallyl sulfide and berberine inhibit the growth of methanogens, nitrates competitively preoccupy the hydrogen needed for methane production, and eucalyptus oil reduces methane production, respectively, by reducing the number of protozoa.
- the synergistic effect showed more than 45% of methane reduction rate.
- allene, nitrate, berberine and eucalyptus oil mixed additives are added at a ratio of 0.3 wt% or 0.5 wt% relative to 10 g of the substrate, and the total gas generation and methane generation at the same time every day for 3 days as in the control. , pH value was measured. Every time the addition content was verified, the methane reduction rate was measured after a period of adaptation with freshly collected rumen liquid, and the measurement results are shown in Table 7 and FIG. 7.
- the milking cow specification test was carried out in the methane of diallyl sulfide (DADS), nitrate (Nitrate), berberine and eucalyptus oil mixed additive composition (Power-green premix) mentioned in Example 6)
- DADS diallyl sulfide
- Nirate nitrate
- berberine berberine
- eucalyptus oil mixed additive composition Power-green premix
- Laser methane detector is a device that detects methane in the air by near infrared absorption spectroscopy using semiconductor laser. Laser methane detector detection distance was 30 m, methane density data was measured for 5 minutes at 1 second intervals.
- the mixed additive composition Power-green premix was confirmed that there is an effect of reducing the methane generation in the ruminant ruminant of the ruminant in vitro as well as in vivo test.
- the treatment group fed the mixed additive composition Power-green premix did not reduce the flow rate compared to the control, rather the flow rate increased by more than 1.1%.
- feeding 25 g / d to 50 g / d or less of the mixed additive composition Power-green premix does not adversely affect the fermentability in the rumen.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Birds (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
Description
연속 배양 | 황화디알릴(DADS) 0.1 wt% | 황화디알릴(DADS) 0.2 wt% | 황화디알릴(DADS) 0.4 wt% |
pH | 6.71 | 6.63 | 6.58 |
메탄 저감율(%) | 30.5 | 65.2 | 65.9 |
연속 배양 | 알린(Alliin) 0.5 wt% |
pH | 6.84 |
메탄 저감율(%) | 21.9 |
연속 배양 | 질산염(nitrate) 0.35 wt% | 질산염(nitrate) 0.5 wt% | 질산염(nitrate) 1.0 wt% |
pH | 6.63 | 6.65 | 6.57 |
메탄 저감율(%) | 14.0 | 31.0 | 8.1 |
연속 배양 | 유칼립투스 오일(Eucalyptus oil) 0.5 wt% |
pH | 6.46 |
메탄 저감율(%) | 20.5 |
연속 배양 | 베르베린(0.1 wt%) | 베르베린(0.2 wt%) | 베르베린(0.5 wt%) |
pH | 6.43 | 6.44 | 6.49 |
메탄 저감율(%) | 6.7 | 12.4 | 45.5 |
연속 배양 | 황화디알릴:질산염:베르베린:유칼립투스 = 0.05 wt%:0.1 wt%:0.05 wt%:0.1 wt% | 황화디알릴:질산염:베르베린:유칼립투스 = 0.05 wt%:0.2 wt%:0.05 wt%:0.2 wt% |
pH | 6.72 | 6.72 |
메탄저감율(%) | 45.2 | 56.2 |
연속 배양 | 알린:질산염:베르베린:유칼립투스 = 0.05 wt%:0.1 wt%:0.05 wt%:0.1 wt% | 알린:질산염:베르베린:유칼립투스 = 0.05 wt%:0.2 wt%:0.05 wt%:0.2 wt% |
pH | 6.68 | 6.69 |
메탄 저감율(%) | 22.0 | 30.2 |
대조구 (20두) | 처리구 (20두) | |
1구간(2주) | Power-green premix 0g/d | Power-green premix 25g/d |
2구간(2주) | Power-green premix 0g/d | Power-green premix 50g/d |
처리구 | 메탄저감효과(%) |
1구간(Power-green premix 25g/d) | 23.1 |
2구간(Power-green premix 50g/d) | 36.6 |
그룹정보 | 유량(kg) | |||
그룹 | 산차 | 비유일수 | 1구간 | 2구간 |
대조구 | 2.4 | 225 | 30.6 | 29.4 |
처리구 | 2.5 | 243 | 31.2 | 29.8 |
유량개선율(%) | 2.0% | 1.1% |
Claims (9)
- 알린(Alliin) 및 베르베린(Berberine)으로 구성되는 군으로부터 선택되는 하나 이상의 성분을 포함하는 메탄 생성 저감용 사료 첨가제 조성물.
- 제1항에 있어서, 상기 조성물은 건조사료 중량 대비 알린이 0.005 wt% 내지 5 wt% 또는 베르베린이 0.005 wt% 내지 5 wt%로 첨가되도록 하는 양으로 사료에 첨가되는 것인 메탄 생성 저감용 사료 첨가제 조성물.
- 제1항에 있어서, 상기 조성물은 황화디알릴(Diallyl disulfide, DADS), 질산염(Nitrate) 및 유칼립투스 오일(Eucalyptus oil)로 구성되는 군으로부터 선택되는 하나 이상의 성분을 추가로 포함하는 메탄 생성 저감용 사료 첨가제 조성물.
- 제3항에 있어서, 상기 조성물은 건조 사료 중량 대비 황화디알릴이 0.005 wt% 내지 4 wt%, 알린이 0.005 wt% 내지 5 wt%, 질산염이 0.01 wt% 내지 0.5 wt%, 베르베린이 0.005 wt% 내지 5 wt% 또는 유칼립투스 오일이 0.01 wt% 내지 5 wt%로 첨가되도록 하는 양으로 사료에 첨가되는 것인 메탄 생성 저감용 사료 첨가제 조성물.
- 제3항에 있어서, 상기 조성물은 황화디알릴, 질산염, 베르베린 및 유칼립투스 오일을 포함하는 것을 특징으로 하는 메탄 생성 저감용 사료 첨가제 조성물.
- 제5항에 있어서, 상기 조성물은 건조 사료 중량 대비 황화디알릴이 0.005 wt% 내지 4 wt%, 질산염이 0.01 wt% 내지 0.5 wt%, 베르베린이 0.005 wt% 내지 5 wt% 및 유칼립투스 오일이 0.01 wt% 내지 5 wt%로 첨가되도록 하는 양으로 사료에 첨가되는 것인 메탄 생성 저감용 사료 첨가제 조성물.
- 제3항에 있어서, 상기 조성물은 알린, 질산염, 베르베린 및 유칼립투스 오일을 포함하는 것을 특징으로 하는 메탄 생성 저감용 사료 첨가제 조성물.
- 제7항에 있어서, 상기 조성물은 건조 사료 중량 대비 알린이 0.005 wt% 내지 5 wt%, 질산염이 0.01 wt% 내지 0.5 wt%, 베르베린이 0.005 wt% 내지 5 wt% 및 유칼립투스 오일이 0.01 wt% 내지 5 wt%로 첨가되도록 하는 양으로 사료에 첨가되는 것인 메탄 생성 저감용 사료 첨가제 조성물.
- 제1항 내지 제8항 중 어느 하나의 조성물을 개체에 투여하는 단계를 포함하는 메탄 생성 저감 방법.
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA2951015A CA2951015C (en) | 2014-06-02 | 2015-06-02 | Feed additive composition for reducing methane gas produced by ruminant animals |
US15/315,593 US10440975B2 (en) | 2014-06-02 | 2015-06-02 | Feed additive composition for reducing methane gas produced by ruminant animals |
CN201580041806.8A CN106572683A (zh) | 2014-06-02 | 2015-06-02 | 用于减少由反刍动物产生的甲烷气体的饲料添加剂组合物 |
EP15803380.3A EP3150073A4 (en) | 2014-06-02 | 2015-06-02 | Feed additive composition for reducing methane production of ruminants |
NZ727563A NZ727563A (en) | 2014-06-02 | 2015-06-02 | Feed additive composition for reducing methane production of ruminants |
JP2016570820A JP6620296B2 (ja) | 2014-06-02 | 2015-06-02 | 反芻動物のメタン生成低減用飼料添加剤組成物 |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020140067168A KR101656929B1 (ko) | 2014-06-02 | 2014-06-02 | 반추동물의 메탄 생성 저감용 사료 첨가제 조성물 |
KR10-2014-0067168 | 2014-06-02 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2015186956A1 true WO2015186956A1 (ko) | 2015-12-10 |
Family
ID=54766981
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/KR2015/005541 WO2015186956A1 (ko) | 2014-06-02 | 2015-06-02 | 반추동물의 메탄 생성 저감용 사료 첨가제 조성물 |
Country Status (8)
Country | Link |
---|---|
US (1) | US10440975B2 (ko) |
EP (1) | EP3150073A4 (ko) |
JP (2) | JP6620296B2 (ko) |
KR (1) | KR101656929B1 (ko) |
CN (1) | CN106572683A (ko) |
CA (1) | CA2951015C (ko) |
NZ (1) | NZ727563A (ko) |
WO (1) | WO2015186956A1 (ko) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2018176093A1 (en) * | 2017-03-28 | 2018-10-04 | Iriccorgpharm Pty Ltd | Berbine alkaloid formulations in the prevention and/or treatment of infectious disease |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10729132B2 (en) * | 2015-09-18 | 2020-08-04 | Environmetal Intellectual Property, Inc. | Xylophage control using antimethanogenic reagents |
WO2018220338A1 (en) * | 2017-06-01 | 2018-12-06 | Mootral Sa | Animal feed supplement |
CN110506848A (zh) * | 2019-09-25 | 2019-11-29 | 兰州大学 | 一种降低反刍动物甲烷排放的饲料和方法 |
PL4037666T3 (pl) | 2020-12-08 | 2024-08-19 | Ruminant Biotech Corp Limited | Ulepszenia w urządzeniach i sposobach dostarczania substancji zwierzętom |
CN113367236B (zh) * | 2021-06-17 | 2022-11-01 | 中国农业科学院北京畜牧兽医研究所 | 调控反刍动物瘤胃微生物发酵的植物源天然化合物及其应用 |
CN113575768B (zh) * | 2021-06-23 | 2023-09-15 | 中国农业科学院北京畜牧兽医研究所 | 用于调控反刍动物瘤胃发酵气体产量的组合物及其用途 |
US12084610B2 (en) | 2022-07-01 | 2024-09-10 | Arkea Bio Corp. | Compositions and methods for reducing deleterious atmospheric gas emissions from flooded ecosystems |
WO2024039863A1 (en) * | 2022-08-19 | 2024-02-22 | Arkea Bio Corp. | Continuous methane monitoring device |
KR20240107875A (ko) * | 2022-12-30 | 2024-07-09 | 대한민국(농촌진흥청장) | 크레아티놀포스페이트(Creatinolfosfate)을 포함하는 반추동물의 메탄 생성 저감용 조성물 |
KR20240107872A (ko) * | 2022-12-30 | 2024-07-09 | 대한민국(농촌진흥청장) | 안지오텐신 iii을 포함하는 반추동물의 메탄 생성 저감용 조성물 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101107969A (zh) * | 2007-08-15 | 2008-01-23 | 窦观一 | 替代粮食减少温室气体的节粮型饲料 |
WO2009150264A1 (es) * | 2008-06-12 | 2009-12-17 | Carotenoid Technologies, S.A. | Procedimiento para mejorar el estado general de los rumiantes |
KR20110036470A (ko) * | 2009-10-01 | 2011-04-07 | 강원대학교산학협력단 | 반추동물의 메탄가스 생성 저감용 사료 및 저감 방법 |
KR20120104309A (ko) * | 2009-12-11 | 2012-09-20 | 디에스엠 아이피 어셋츠 비.브이. | 반추동물에서의 메탄 방출을 감소시키기고/감소시키거나 반추동물 수익성을 개선시키기 위한 사료중의 니트로옥시 알칸산 및 이의 유도체 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
PT1663225E (pt) | 2003-08-28 | 2011-09-05 | Australian Biomedical Company Pty Ltd | Composições para aplicações veterinárias e médicas |
KR100679153B1 (ko) | 2004-08-26 | 2007-02-05 | 진주산업대학교 산학협력단 | 반추동물의 메탄 생성량을 억제하기 위한 사료조성물 |
CA2727811A1 (en) * | 2008-06-13 | 2009-12-17 | Proyecto De Biomedicina Cima, S.L. | Conjugates for the administration of biologically active compounds |
CA2771903A1 (fr) * | 2009-07-13 | 2011-01-20 | Pancosma Sa | Additif alimentaire contenant de l'eugenol, du cinnamaldehyde et un extrait d'alliacee |
UY32802A (es) * | 2009-07-23 | 2011-01-31 | Provimi Holding B V | Composiciones para reducir la metanogénesis gastrointestinal en rumiantes |
AU2011347802B2 (en) | 2010-12-20 | 2015-05-07 | Dsm Ip Assets B.V. | Use of nitrooxy organic molecules in feed for reducing methane emission in ruminants, and/or to improve ruminant performance |
CN102743420A (zh) | 2012-06-06 | 2012-10-24 | 上海交通大学 | 改善肠道菌群结构的方法及应用 |
-
2014
- 2014-06-02 KR KR1020140067168A patent/KR101656929B1/ko active IP Right Grant
-
2015
- 2015-06-02 EP EP15803380.3A patent/EP3150073A4/en not_active Withdrawn
- 2015-06-02 CA CA2951015A patent/CA2951015C/en not_active Expired - Fee Related
- 2015-06-02 NZ NZ727563A patent/NZ727563A/en not_active IP Right Cessation
- 2015-06-02 JP JP2016570820A patent/JP6620296B2/ja not_active Expired - Fee Related
- 2015-06-02 US US15/315,593 patent/US10440975B2/en active Active
- 2015-06-02 WO PCT/KR2015/005541 patent/WO2015186956A1/ko active Application Filing
- 2015-06-02 CN CN201580041806.8A patent/CN106572683A/zh active Pending
-
2018
- 2018-10-25 JP JP2018200864A patent/JP2019058170A/ja not_active Withdrawn
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101107969A (zh) * | 2007-08-15 | 2008-01-23 | 窦观一 | 替代粮食减少温室气体的节粮型饲料 |
WO2009150264A1 (es) * | 2008-06-12 | 2009-12-17 | Carotenoid Technologies, S.A. | Procedimiento para mejorar el estado general de los rumiantes |
KR20110036470A (ko) * | 2009-10-01 | 2011-04-07 | 강원대학교산학협력단 | 반추동물의 메탄가스 생성 저감용 사료 및 저감 방법 |
KR20120104309A (ko) * | 2009-12-11 | 2012-09-20 | 디에스엠 아이피 어셋츠 비.브이. | 반추동물에서의 메탄 방출을 감소시키기고/감소시키거나 반추동물 수익성을 개선시키기 위한 사료중의 니트로옥시 알칸산 및 이의 유도체 |
Non-Patent Citations (2)
Title |
---|
PATRA, AMLAN K. ET AL.: "Effects of Essential Oils on Methane Production and Fermentation by, and Abundance and Diversity of, Rumen Microbial Populations.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 78, no. 12, June 2012 (2012-06-01), pages 4271 - 8280, XP055240143 * |
See also references of EP3150073A4 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2018176093A1 (en) * | 2017-03-28 | 2018-10-04 | Iriccorgpharm Pty Ltd | Berbine alkaloid formulations in the prevention and/or treatment of infectious disease |
CN110709080A (zh) * | 2017-03-28 | 2020-01-17 | Irp健康股份有限公司 | 小檗碱生物碱制剂在预防和/或治疗传染病中的作用 |
Also Published As
Publication number | Publication date |
---|---|
US20170196240A1 (en) | 2017-07-13 |
CA2951015C (en) | 2019-03-26 |
JP2019058170A (ja) | 2019-04-18 |
NZ727563A (en) | 2018-05-25 |
CA2951015A1 (en) | 2015-12-10 |
KR101656929B1 (ko) | 2016-09-13 |
KR20150139142A (ko) | 2015-12-11 |
CN106572683A (zh) | 2017-04-19 |
JP6620296B2 (ja) | 2019-12-18 |
US10440975B2 (en) | 2019-10-15 |
JP2017522008A (ja) | 2017-08-10 |
EP3150073A1 (en) | 2017-04-05 |
EP3150073A4 (en) | 2018-01-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2015186956A1 (ko) | 반추동물의 메탄 생성 저감용 사료 첨가제 조성물 | |
DE3910663A1 (de) | 5-alkylchinoloncarbonsaeuren | |
EP0210513A1 (de) | Zubereitungen zur Behandlung von Mycoplasmosen und bakteriellen Erkrankungen bei Geflügel | |
WO2015020347A1 (ko) | 팜 가공 부산물을 이용한 섬유질 발효사료 제조 설비 및 제조 방법 | |
WO2016195369A1 (ko) | 사료용 유지 조성물, 이를 이용한 성장 촉진용 사료 첨가제, 가축용 사료 조성물 및 가축 사육 방법 | |
EP0326916A2 (de) | Chinolon- und Naphthyridoncarbonsäurederivate, Verfahren zu ihrer Herstellung und sie enthaltende antibakterielle Mittel und Futterzusatzstoffe | |
ES2023609A6 (es) | Procedimiento para la obtencion del 2-(((3-metil -4-(2,2,2-trifluoroetoxi) -2-piridinil) metilisulfinil) -1h-bemzimidazol. | |
DE4032560A1 (de) | 7-(2,7-diazabicyclo(3.3.0)octyl)-3-chinolon- und -naphtyridoncarbonsaeure-derivate | |
WO2020235774A1 (ko) | 내동성 살균제 조성물 및 그 제조방법 | |
DD283381A5 (de) | Verfahren zur herstellung einer verbindung | |
WO2022139529A1 (en) | Composition for preventing, improving or treating gastritis or peptic ulcer comprising extract of cinnamomum cassia, fraction of said extract, isolate of said fraction or compounds isolated therefrom | |
ES8502116A1 (es) | Un compuesto de pirazolpiridina. | |
DE3910920A1 (de) | Enantiomerenreine 7-(3-amino-1-pyrrolidinyl)-chinolon- und -naphthyridoncarbonsaeuren | |
ATE54311T1 (de) | Ein neues ansamycin-antibiotikum, ein mikrobielles verfahren zu seiner herstellung und seine verwendung als arzneimittel. | |
CS649787A2 (en) | Method of new 4,5-dihydrooxazole derivatives production | |
WO2016111574A1 (ko) | 신규한 고리형 뎁시펩타이드계 화합물, 이의 제조방법 및 이를 유효성분으로 함유하는 항균용 약학적 조성물 | |
US5141749A (en) | Tetraamides and method for improving feed utilization | |
DE19652219A1 (de) | Verwendung von 7-(1-Aminomethyl-2-oxa-7-azabicyclo[3.3.0]oct-7-yl)-chinolon- und naphthyridoncarbonsäure-Derivaten zur Therapie von Helicobacter-pylori-Infektionen und den damit assoziierten gastroduodenalen Erkrankungen | |
WO2024106663A1 (ko) | 티아민 삼인산을 포함하는 반추동물의 메탄 생성 저감용 조성물 | |
WO2024143729A1 (ko) | 안지오텐신 iii을 포함하는 반추동물의 메탄 생성 저감용 조성물 | |
DE3363450D1 (en) | Pyridobenzodiazepinones, process for their preparation and pharmaceutical compositions containing them | |
JPS5770889A (en) | 1-ethyl-1,8-naphthyridine derivative and salt thereof | |
JPS5742795A (en) | Preparation of fuel using excrements and saw dust | |
WO2012118286A1 (en) | Novel compound isolated from kaempferia pandurata roxb and use thereof as antiviral agent | |
WO2011090345A2 (ko) | 플루페나믹산을 유효성분으로 포함하는 신규한 항생제 조성물 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 15803380 Country of ref document: EP Kind code of ref document: A1 |
|
ENP | Entry into the national phase |
Ref document number: 2951015 Country of ref document: CA Ref document number: 2016570820 Country of ref document: JP Kind code of ref document: A |
|
WWE | Wipo information: entry into national phase |
Ref document number: 15315593 Country of ref document: US |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
REEP | Request for entry into the european phase |
Ref document number: 2015803380 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2015803380 Country of ref document: EP |