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WO2015163165A1 - Glutathione-containing antioxidant composition - Google Patents

Glutathione-containing antioxidant composition

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Publication number
WO2015163165A1
WO2015163165A1 PCT/JP2015/061231 JP2015061231W WO2015163165A1 WO 2015163165 A1 WO2015163165 A1 WO 2015163165A1 JP 2015061231 W JP2015061231 W JP 2015061231W WO 2015163165 A1 WO2015163165 A1 WO 2015163165A1
Authority
WO
Grant status
Application
Patent type
Prior art keywords
antioxidant
glutathione
composition
containing
present
Prior art date
Application number
PCT/JP2015/061231
Other languages
French (fr)
Japanese (ja)
Inventor
中川智寛
梶直人
徳力基之
Original Assignee
興人ライフサイエンス株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

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Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILET PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • A23L33/18Peptides; Protein hydrolysates
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K8/00Cosmetics or similar toilet preparations
    • A61K8/18Cosmetics or similar toilet preparations characterised by the composition
    • A61K8/30Cosmetics or similar toilet preparations characterised by the composition containing organic compounds
    • A61K8/64Proteins; Peptides; Derivatives or degradation products thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/52Stabilizers
    • A61K2800/522Antioxidants; Radical scavengers

Abstract

[Problem] The present invention addresses the problem of providing a composition useful in the fields of food, cosmetics and pharmaceuticals, said composition being capable of synergistically enhancing antioxidant action. [Solution] The present invention is achieved by preparing an antioxidant composition by combining a specific quantity of glutathione with a general antioxidant so as to prepare an antioxidant composition, thereby synergistically enhancing the antioxidant action of the antioxidant composition. Specifically, the composition contains an antioxidant (A) and glutathione (B), which are contained at a ratio of 1 to 5 parts by weight of component (B) to 100 parts by weight of component (A).

Description

Glutathione-containing antioxidant compositions

The present invention is related to the formulation of general antioxidants glutathione. Synergistic effect of the antioxidant activity is obtained by blending of the present invention. The compositions according to the formulation of the present invention, foods, cosmetics, and is used in the pharmaceutical field.

In modern society, human body exposure to ultraviolet light, an unbalanced diet, pesticides, insecticides, food additives, detergents, constantly subjected to and, free radicals and active oxygen in the living body overproduction oxidative stress due to various factors environmental pollutants It has become easily.

Moderate free radical or active oxygen in the body, plays an important role in host defense, excess of free radicals and active oxygen when generating, has been found to result in various adverse effects in the living body. For example, active oxygen converts the unsaturated fatty acid of human cell membrane lipid peroxidation, it causes the aging of cells. In addition, active oxygen generated in the liver, causing a decrease in liver function, or become tired easily, comes out symptoms such as motivated or lost. Further, the active oxygen in the skin by ultraviolet exposure generates excessive, or cause spots and freckles, by elasticity and gloss of the skin is or reduce, skin aging is induced. And further, the active oxygen reacts with LDL cholesterol in vivo to produce a oxidized LDL, which is arteriosclerosis, myocardial infarction, it is also known that a factor of diseases such as cerebral infarction.

For this to be removed or eliminate active oxygen generated excessively in the body, substances having an antioxidant effect are known to be useful (for example, see Patent Document 1). Currently, as a substance having an antioxidative action it is known numerous substances, in order to more effectively remove or eliminate active oxygen in the body, important to enhance the antioxidant action of the substance it is considered to be.

Glutathione, glutamic acid, cysteine, a tripeptide three amino acids that glycine is bound, plays an important role in vivo. For example, and acts as an antioxidant to protect cells from the active oxygen and peroxide substance in cells, such as detoxification is known to discharge foreign matter out of the cell. Also in skin, active oxygen becomes a cause of melanin pigment is thought to stains, pigmentation is suppressed by the antioxidant action of glutathione.

JP-T 2005-503365 JP

An object of the present invention, synergistically exert antioxidant effects in vivo, the food field and cosmetics, it is to provide compositions useful in various fields such as pharmaceuticals.

Was intensive studies to solve the above problems, by the antioxidant composition by combining specific amounts of glutathione for common antioxidants, antioxidant of the antibody oxide material is synergistically enhanced heading the Rukoto, it has led to the completion of the present invention.

That is, the present invention is,
(1) antioxidant (A) and containing glutathione (B), (A) relative to 100 parts by weight of component glutathione, characterized in that it contains a proportion of 1 to 5 parts by weight of component (B) containing antioxidant composition,
(2) (1) Food products containing glutathione-containing antioxidant composition according to,
(3) (1) Cosmetics containing glutathione-containing antioxidant composition according to,
(4) (1) Pharmaceutical containing glutathione-containing antioxidant composition according to,
It is intended to provide.

According to the present invention, those obtained by blending a small amount of glutathione in general antioxidants, greater than additive effect of the combined respective antioxidant, shows a synergistic effect. That is, it is possible to synergistically promote antioxidant activity of common antioxidants The inclusion of glutathione.

In the present invention, common antioxidants (A) but is not particularly limited, for example, ascorbic acid and derivatives thereof, erythorbic acid and derivatives thereof, tocopherol and derivatives thereof, from bilberries and Santa berries like grapes berries, carotene, lycopene, propyl gallate, tannic acid, catechins such as epigallocatechin, polyphenols such as anthocyanins, butylhydroxytoluene, butylhydroxyanisole, p- hydroxyanisole, and their salts. Among them, food hygiene, it is desirable to be acceptable pharmaceutically or cosmetically.

The glutathione (B) used in the present invention, glutamic acid, cysteine, glycine tripeptide bound, reduced glutathione is desirable.
In the form of glutathione may be purified glutathione, or may be a material containing a large amount of glutathione. Examples include such as glutathione-rich yeast or the yeast extract.

Antioxidant compositions of the present invention are those which contain antioxidants (A) and glutathione (B). Their desired mixing ratio varies depending on the form and application of the said composition, the proportions of expressing more excellent antioxidant action is desirable. For example, when measuring the radical-scavenging rate, when a "predictive value (%) = erasure rate of glutathione (%) + erasing ratio of each raw material (%)", the value of (measured value) / (estimated value) 1 .10 or higher is desirable, and more desirably proportions becomes 1.30 or more. Specifically, in a proportion as the glutathione (B) 1 ~ 5 parts by weight per 100 parts by weight of antioxidant (A).

By using a combination of a small amount of glutathione in general antioxidants, can synergistically promote antioxidant activity of the antibody oxidants, as these antioxidant activity composition, food, cosmetics, it can be applied to pharmaceuticals.

The food or drink of the present invention refers to blended the composition, food, beverages, luxury items, supplements, those ingested orally. Its form is not limited in particular, those bread, can be a noodle like main dishes, cheese, ham, sausage, which can be a fish processed products side dishes, fruit juice drinks, carbonated drinks, beverages such as milk drink, it is possible to cookies, cakes, jelly, pudding, candy, the preference Hinto such as yogurt. Further, the form of the supplement is also not particularly limited, it is also possible to take a tablet, capsule, soft capsule, a nutritional drink form.

The amount of the composition in the food and drink is not particularly limited as long as it has an antioxidant activity, for example antioxidants (A) and glutathione (B) the total amount is 10 pg ~ 20 g to mass 100g of food or drink it may be included in such that. Of these 100 pg ~ 2 g are preferred, 10 ng ~ 500 mg are further preferred.

The cosmetics of the present invention, refers lotion containing the composition, emulsion, foundation, and lipstick.

The amount of the composition in a cosmetic is not limited in particular, for example, the total amount of antioxidants (A) and glutathione (B) may be contained 10 pg ~ 20 g to mass 100g cosmetics. Of these 100 pg ~ 2 g are preferred, 10 ng ~ 500 mg are further preferred.

Also with the medicament of the present invention, internal medicine, include external preparations, such as tablets formulated with the composition, powders, lotions, creams, ointments, sprays, refers to a shape, such as patches (materials), the present invention as long as it can exhibit the aimed effects of may be in any form.

The amount of the composition in the medicament is not limited in particular, it may be contained 10 pg ~ 20 g to mass 100g of total weight medicines such as anti-oxidants (A) and glutathione (B). Of these 100 pg ~ 2 g are preferred, 10 ng ~ 500 mg are further preferred.

The present invention will be described more specifically below, the present invention is not limited thereto.

A substance having antioxidant activity which is generally used as a food and drink, a 1/20 to 1/40 times the weight of glutathione were mixed to evaluate its antioxidant power. Specifically, Santa berries are antioxidants, bilberry, ascorbic acid, were mixed respectively catechin and reduced glutathione to prepare an aqueous solution at a concentration shown in Table 1, the DPPH radical scavenging activity measurement, evaluation of antioxidant activity It was carried out.

(DPPH radical scavenging activity measurement)
Samples were concentration adjustment with water, it was added in 100μL in 96well microplate. Thereto was added 0.2mM of 1,1-diphenyl-2-picrylhydrazyl (DPPH) ethanol solution by 100 [mu] L, were mixed well and allowed to stand for 30 minutes at room temperature, in the dark. It was then measured absorbance 517nm of (As). Absorbance upon addition of water in place of the sample solution (Ac) as a control, the radical-scavenging rate of the sample was determined by the following equation.
Radical scavenging rate (%) = {1- (As) / (Ac)} × 100

Predicted value of the erasure rate when a combination of glutathione and the antioxidant were determined by the following equation.
Predictive value (%) = erasure rate of glutathione (%) + erasing ratio of each raw material (%)

Thus, by comparing the actual measurement value and the predicted value, and evaluate the effect on the erase ratio of the combined system. (Found) / synergistic effect in the case of (predicted value) ratio is 1.10 or more, the additive effect at 0.91 to 1.09, it is determined that the cancellation effect at 0.90 or less.

(result)
Table 1 shows the results of the above Examples. In the display of the table ◎ has a synergistic effect, 〇 is additive effect, × denotes an offsetting effect.

Figure JPOXMLDOC01-appb-T000001

As shown in Table 1, In all test groups, antioxidant action by blending a small amount of glutathione for each antioxidant is synergistically enhanced.

In order to confirm the synergistic effect comes desired blending ratio of the antioxidant glutathione, blended ascorbic acid and glutathione as shown in Table 2, were DPPH radical scavenging activity measured in the same manner as in Example 1.

Figure JPOXMLDOC01-appb-T000002

As shown in Table 2, although synergistic antioxidant effect is observed with glutathione 5 parts by weight per 100 parts by weight of ascorbic acid, glutathione 12.5 parts by weight per 100 parts by weight of ascorbic acid, or more is blended, antioxidant activity is reduced.
That is, if the amount of glutathione is excessive, there is no combination effect, antioxidant by blending a small amount of glutathione against antioxidants we have found to be synergistically enhanced.

Claims (4)

  1. A composition comprising a glutathione antioxidant, the relative antioxidant to 100 parts by weight of glutathione is contained in a proportion of 1 to 5 parts by weight, containing glutathione antioxidant compositions.
  2. Food or drink containing claim 1 containing glutathione antioxidant composition.
  3. Cosmetics containing claim 1 containing glutathione antioxidant composition.
  4. Medicaments containing glutathione-containing antioxidant composition according to claim 1.
PCT/JP2015/061231 2014-04-22 2015-04-10 Glutathione-containing antioxidant composition WO2015163165A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2014-088462 2014-04-22
JP2014088462 2014-04-22

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2016514860A JPWO2015163165A1 (en) 2014-04-22 2015-04-10 Glutathione-containing antioxidant compositions

Publications (1)

Publication Number Publication Date
WO2015163165A1 true true WO2015163165A1 (en) 2015-10-29

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2015/061231 WO2015163165A1 (en) 2014-04-22 2015-04-10 Glutathione-containing antioxidant composition

Country Status (2)

Country Link
JP (1) JPWO2015163165A1 (en)
WO (1) WO2015163165A1 (en)

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000060482A (en) * 1998-06-11 2000-02-29 Fancl Corp Food composition
JP2002507974A (en) * 1997-06-27 2002-03-12 サイオン・インターナショナル,インコーポレイテッド Oral antioxidants Formulation
US20020110604A1 (en) * 2000-08-11 2002-08-15 Ashni Naturaceuticals, Inc. Composition exhibiting synergistic antioxidant activity
JP2002233332A (en) * 2001-02-05 2002-08-20 Nakagami Corporation:Kk Gingko leaf extract-containing composition with high antioxidative activity
US20060008487A1 (en) * 2004-07-12 2006-01-12 Sivak Hannah N Method of skin care and/or treatment using thioredoxin
US20070082064A1 (en) * 2005-10-12 2007-04-12 Krawitz Paul L Nutritional or dietary supplement for the treatment of macular degeneration
WO2010112071A1 (en) * 2009-04-01 2010-10-07 Phf S.A. Combination of astaxanthin, antioxidants and colouring agents with sunscreen effect to be administered orally
US20120003378A1 (en) * 2010-07-02 2012-01-05 Rbc Life Sciences, Inc. Dietary supplement
CN103251927A (en) * 2013-04-11 2013-08-21 深圳市百汇生物技术有限公司 Composition containing bilberry extract

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002507974A (en) * 1997-06-27 2002-03-12 サイオン・インターナショナル,インコーポレイテッド Oral antioxidants Formulation
JP2000060482A (en) * 1998-06-11 2000-02-29 Fancl Corp Food composition
US20020110604A1 (en) * 2000-08-11 2002-08-15 Ashni Naturaceuticals, Inc. Composition exhibiting synergistic antioxidant activity
JP2002233332A (en) * 2001-02-05 2002-08-20 Nakagami Corporation:Kk Gingko leaf extract-containing composition with high antioxidative activity
US20060008487A1 (en) * 2004-07-12 2006-01-12 Sivak Hannah N Method of skin care and/or treatment using thioredoxin
US20070082064A1 (en) * 2005-10-12 2007-04-12 Krawitz Paul L Nutritional or dietary supplement for the treatment of macular degeneration
WO2010112071A1 (en) * 2009-04-01 2010-10-07 Phf S.A. Combination of astaxanthin, antioxidants and colouring agents with sunscreen effect to be administered orally
US20120003378A1 (en) * 2010-07-02 2012-01-05 Rbc Life Sciences, Inc. Dietary supplement
CN103251927A (en) * 2013-04-11 2013-08-21 深圳市百汇生物技术有限公司 Composition containing bilberry extract

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