WO2015152703A3 - Macropuce pour la détection dans des échantillons environnementaux et biologiques de microorganismes entéropathogènes - Google Patents

Macropuce pour la détection dans des échantillons environnementaux et biologiques de microorganismes entéropathogènes Download PDF

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Publication number
WO2015152703A3
WO2015152703A3 PCT/MX2015/000051 MX2015000051W WO2015152703A3 WO 2015152703 A3 WO2015152703 A3 WO 2015152703A3 MX 2015000051 W MX2015000051 W MX 2015000051W WO 2015152703 A3 WO2015152703 A3 WO 2015152703A3
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WO
WIPO (PCT)
Prior art keywords
escherichia coli
array
oligonucleotides
microarray
detection
Prior art date
Application number
PCT/MX2015/000051
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English (en)
Spanish (es)
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WO2015152703A2 (fr
Inventor
Valeria Francisca Leopoldina de María de Guadalupe SOUZA SALDIVAR
Luis Enrique EGUIARTE FRUNS
Andrea GONZÁLEZ GONZÁLEZ
Gabriela DELGADO SAPIEN
Maria del Rosario MORALES ESPINOSA
Luna Luisa SANCHEZ REYES
Cecilia Teresa XIMENEZ GARCIA
René CERRITOS FLORES
Valeria ZEMEÑO LEÓN
Luis David ALCARAZ PERAZA
Original Assignee
Universidad Nacional Autónoma de México
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Publication date
Application filed by Universidad Nacional Autónoma de México filed Critical Universidad Nacional Autónoma de México
Publication of WO2015152703A2 publication Critical patent/WO2015152703A2/fr
Publication of WO2015152703A3 publication Critical patent/WO2015152703A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne le domaine du diagnostic moléculaire multi-étiologique et des maladies émergentes au moyen de puces à ADN, plus spécifiquement l'invention utilise des oligonucléotides inclus dans une puce, pour la détection de microorganismes qui peuvent provoquer la maladie diarrhéique. La puce contient les oligonucléotides qui correspondent à Escherichia coli entéroaggrégative, Eschenchia coli entérohémorragique, Escherichia coli entérotoxigénique, Escherichia coli entéropathogène, Escherichia coli entéroinvasive, Giardia intestinalis, Blastocystishominis, Entamoeba histolytica/dispar, Cryptosporidiumparvum, Shigella ssp. Escherichia coli uropatogénique, Salmonella entérica, Vibrio cholerae, entre autres pathogènes provoquant des maladies gastriques chez l'homme ou l'animal. La puce consiste à immobiliser sur un support, lesdits oligonucléotides une fois amplifiés par la technique PCR. La puce peut être une micropuce ou une macropuce et il est inclus dans les modalités, sa disposition à la manière d'une biopuce, qu'elle soit macropuce ou micropuce.
PCT/MX2015/000051 2014-04-01 2015-03-25 Macropuce pour la détection dans des échantillons environnementaux et biologiques de microorganismes entéropathogènes WO2015152703A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MX2014003938A MX2014003938A (es) 2014-04-01 2014-04-01 Macroarreglos para deteccion en muestras ambientales y biologicas de microorganismos enteropatogenos.
MXMX/A/2014/003938 2014-04-01

Publications (2)

Publication Number Publication Date
WO2015152703A2 WO2015152703A2 (fr) 2015-10-08
WO2015152703A3 true WO2015152703A3 (fr) 2016-01-14

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/MX2015/000051 WO2015152703A2 (fr) 2014-04-01 2015-03-25 Macropuce pour la détection dans des échantillons environnementaux et biologiques de microorganismes entéropathogènes

Country Status (2)

Country Link
MX (1) MX2014003938A (fr)
WO (1) WO2015152703A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115830068B (zh) * 2022-11-29 2023-06-20 中国环境科学研究院 基于污染路径识别的污染溯源大数据模型

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111004865A (zh) * 2018-10-05 2020-04-14 福又达生物科技股份有限公司 鱼病原体检测方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002059355A2 (fr) * 2001-01-25 2002-08-01 Tm Bioscience Corporation Polynucleotides utilises comme marqueurs et complements marqueurs, fabrication et utilisation de ces polynucleotides
WO2008140494A2 (fr) * 2006-11-22 2008-11-20 The Board Of Trustees Of Michigan State University Criblage haut débit à l'aide de micro-réseaux
EP2559761A1 (fr) * 2010-04-14 2013-02-20 Toyo Seikan Kaisha, Ltd. Ensemble d'amorces pour pcr, liquide de réaction pour pcr et méthode de détection de bactéries causant des intoxications alimentaires
WO2014001648A1 (fr) * 2012-06-27 2014-01-03 Mobidiag Oy Procédé pour la détermination de la présence de pathogènes provoquant la diarrhée

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002059355A2 (fr) * 2001-01-25 2002-08-01 Tm Bioscience Corporation Polynucleotides utilises comme marqueurs et complements marqueurs, fabrication et utilisation de ces polynucleotides
WO2008140494A2 (fr) * 2006-11-22 2008-11-20 The Board Of Trustees Of Michigan State University Criblage haut débit à l'aide de micro-réseaux
EP2559761A1 (fr) * 2010-04-14 2013-02-20 Toyo Seikan Kaisha, Ltd. Ensemble d'amorces pour pcr, liquide de réaction pour pcr et méthode de détection de bactéries causant des intoxications alimentaires
WO2014001648A1 (fr) * 2012-06-27 2014-01-03 Mobidiag Oy Procédé pour la détermination de la présence de pathogènes provoquant la diarrhée

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
FUKUSHIMA HIROSHI ET AL.: "Simultaneous Screening of 24 Target Genes of Foodborne Pathogens in 35 Foodborne Outbreaks Using Multiplex Real-Time SYBR Green PCR Analysis.", INTERNATIONAL JOURNAL OF MICROBIOLOGY, vol. 2010, 2010, pages 18 pages *
LIU JIE ET AL.: "A laboratory-developed TaqMan Array Card for simultaneous detection of 19 enteropathogens''.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 51, no. 2, 2013, pages 472 - 480, ISSN: 1098-660X *
POIRIER PHILIPPE ET AL.: "Development and evaluation of a real-time PCR assay for detection and quantification of blastocystis parasites in human stool samples: prospective study of patients with hematological malignancies.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 49, no. 3, 2011, pages 975 - 983, ISSN: 1098-660X *
WAWRZYNIAK IVAN ET AL.: "Blastocystis,an unrecognized parasite: an overview of pathogenesis and diagnosis", THERAPEUTIC ADVANCES IN INFECTIOUS DISEASE, vol. 1, no. 5, 2013, pages 167 - 178, ISSN: 2049-9361 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115830068B (zh) * 2022-11-29 2023-06-20 中国环境科学研究院 基于污染路径识别的污染溯源大数据模型

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Publication number Publication date
MX2014003938A (es) 2015-09-30
WO2015152703A2 (fr) 2015-10-08

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