WO2015152703A3 - Microarray for the detection of enteropathogenic microorganisms in environmental and biological samples - Google Patents

Microarray for the detection of enteropathogenic microorganisms in environmental and biological samples Download PDF

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Publication number
WO2015152703A3
WO2015152703A3 PCT/MX2015/000051 MX2015000051W WO2015152703A3 WO 2015152703 A3 WO2015152703 A3 WO 2015152703A3 MX 2015000051 W MX2015000051 W MX 2015000051W WO 2015152703 A3 WO2015152703 A3 WO 2015152703A3
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WO
WIPO (PCT)
Prior art keywords
escherichia coli
array
oligonucleotides
microarray
detection
Prior art date
Application number
PCT/MX2015/000051
Other languages
Spanish (es)
French (fr)
Other versions
WO2015152703A2 (en
Inventor
Valeria Francisca Leopoldina de María de Guadalupe SOUZA SALDIVAR
Luis Enrique EGUIARTE FRUNS
Andrea GONZÁLEZ GONZÁLEZ
Gabriela DELGADO SAPIEN
Maria del Rosario MORALES ESPINOSA
Luna Luisa SANCHEZ REYES
Cecilia Teresa XIMENEZ GARCIA
René CERRITOS FLORES
Valeria ZEMEÑO LEÓN
Luis David ALCARAZ PERAZA
Original Assignee
Universidad Nacional Autónoma de México
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad Nacional Autónoma de México filed Critical Universidad Nacional Autónoma de México
Publication of WO2015152703A2 publication Critical patent/WO2015152703A2/en
Publication of WO2015152703A3 publication Critical patent/WO2015152703A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to the field of multi-etiological molecular diagnosis and diagnosis of emerging diseases through the use of DNA arrays. More specifically, the invention relates to oligonucleotides included in an array for the detection of microorganisms that can cause diarrheal disease. Said array contains the oligonucleotides corresponding to Enteroaggregative Escherichia coli, Enterohemorrhagic Escherichia coli, Enterotoxigenic Escherichia coli, Enteropathogenic Escherichia coli, Enteroinvasive Escherichia coli, Giardia intestinalis, Blastocystis hominis, Entamoeba histolytica/dispar, Cryptosporidium parvum, Shigella spp., Uropathogenic Escherichia coli, Salmonella enterica, Vibrio cholerae, among other pathogens that cause gastric diseases in humans or animals. The array consists in immobilising said oligonucleotides in a support once they have been amplified using the PCR technique. The array can be a microarray or a macroarray, and, in one embodiment, it takes the form of a biochip, either a macrochip or microchip.
PCT/MX2015/000051 2014-04-01 2015-03-25 Microarray for the detection of enteropathogenic microorganisms in environmental and biological samples WO2015152703A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MXMX/A/2014/003938 2014-04-01
MX2014003938A MX2014003938A (en) 2014-04-01 2014-04-01 Microarray for the detection of enteropathogenic microorganisms in environmental and biological samples.

Publications (2)

Publication Number Publication Date
WO2015152703A2 WO2015152703A2 (en) 2015-10-08
WO2015152703A3 true WO2015152703A3 (en) 2016-01-14

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/MX2015/000051 WO2015152703A2 (en) 2014-04-01 2015-03-25 Microarray for the detection of enteropathogenic microorganisms in environmental and biological samples

Country Status (2)

Country Link
MX (1) MX2014003938A (en)
WO (1) WO2015152703A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115830068B (en) * 2022-11-29 2023-06-20 中国环境科学研究院 Pollution tracing big data model based on pollution path identification

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3862443A4 (en) * 2018-10-05 2022-10-12 Schweitzer Biotech Company Ltd. Method for detecting fish pathogen

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002059355A2 (en) * 2001-01-25 2002-08-01 Tm Bioscience Corporation Polynucleotides for use as tags and tag complements, manufacture and use thereof
WO2008140494A2 (en) * 2006-11-22 2008-11-20 The Board Of Trustees Of Michigan State University High throughput screening using microarrays
EP2559761A1 (en) * 2010-04-14 2013-02-20 Toyo Seikan Kaisha, Ltd. Primer set for pcr, reaction liquid for pcr, and method for detecting food poisoning bacteria
WO2014001648A1 (en) * 2012-06-27 2014-01-03 Mobidiag Oy Method for determining the presence of diarrhoea causing pathogens

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002059355A2 (en) * 2001-01-25 2002-08-01 Tm Bioscience Corporation Polynucleotides for use as tags and tag complements, manufacture and use thereof
WO2008140494A2 (en) * 2006-11-22 2008-11-20 The Board Of Trustees Of Michigan State University High throughput screening using microarrays
EP2559761A1 (en) * 2010-04-14 2013-02-20 Toyo Seikan Kaisha, Ltd. Primer set for pcr, reaction liquid for pcr, and method for detecting food poisoning bacteria
WO2014001648A1 (en) * 2012-06-27 2014-01-03 Mobidiag Oy Method for determining the presence of diarrhoea causing pathogens

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
FUKUSHIMA HIROSHI ET AL.: "Simultaneous Screening of 24 Target Genes of Foodborne Pathogens in 35 Foodborne Outbreaks Using Multiplex Real-Time SYBR Green PCR Analysis.", INTERNATIONAL JOURNAL OF MICROBIOLOGY, vol. 2010, 2010, pages 18 pages *
LIU JIE ET AL.: "A laboratory-developed TaqMan Array Card for simultaneous detection of 19 enteropathogens''.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 51, no. 2, 2013, pages 472 - 480, ISSN: 1098-660X *
POIRIER PHILIPPE ET AL.: "Development and evaluation of a real-time PCR assay for detection and quantification of blastocystis parasites in human stool samples: prospective study of patients with hematological malignancies.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 49, no. 3, 2011, pages 975 - 983, ISSN: 1098-660X *
WAWRZYNIAK IVAN ET AL.: "Blastocystis,an unrecognized parasite: an overview of pathogenesis and diagnosis", THERAPEUTIC ADVANCES IN INFECTIOUS DISEASE, vol. 1, no. 5, 2013, pages 167 - 178, ISSN: 2049-9361 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115830068B (en) * 2022-11-29 2023-06-20 中国环境科学研究院 Pollution tracing big data model based on pollution path identification

Also Published As

Publication number Publication date
MX2014003938A (en) 2015-09-30
WO2015152703A2 (en) 2015-10-08

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