WO2015101326A1 - 全自动微生物检测富集系统及其富集方法 - Google Patents

全自动微生物检测富集系统及其富集方法 Download PDF

Info

Publication number
WO2015101326A1
WO2015101326A1 PCT/CN2014/095848 CN2014095848W WO2015101326A1 WO 2015101326 A1 WO2015101326 A1 WO 2015101326A1 CN 2014095848 W CN2014095848 W CN 2014095848W WO 2015101326 A1 WO2015101326 A1 WO 2015101326A1
Authority
WO
WIPO (PCT)
Prior art keywords
filter
enrichment
compartment
cabin
culture
Prior art date
Application number
PCT/CN2014/095848
Other languages
English (en)
French (fr)
Inventor
牛刚
Original Assignee
牛刚
王华山
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 牛刚, 王华山 filed Critical 牛刚
Priority to EP14877171.0A priority Critical patent/EP3091068B1/en
Priority to CA2935299A priority patent/CA2935299C/en
Priority to AU2014375463A priority patent/AU2014375463B2/en
Priority to JP2016561066A priority patent/JP2017502696A/ja
Priority to US15/106,930 priority patent/US20170029760A1/en
Priority to KR1020167020524A priority patent/KR101807984B1/ko
Publication of WO2015101326A1 publication Critical patent/WO2015101326A1/zh

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/44—Multiple separable units; Modules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/18—Rollers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/02—Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting

Definitions

  • the invention relates to the technical field of microbial detection, in particular to an automated automatic microbial detection and enrichment system for enriching a microbial detection process and an enrichment method thereof.
  • microbial detection and enrichment are all manual operations.
  • the sterilization and sterilization, the collection of bacteria, the medium filling and the positive bacteria filling are all independent, and the positive bacteria filling environment is different from the collecting environment. That is to say, the entire process needs to be completed in different environments, and the transmission of each sample to be tested in different environments depends on manual completion, which is highly susceptible to human factors and is highly prone to false positive or false negative results. Affect the accuracy and timeliness of detection.
  • the object of the present invention is to provide a fully automatic microbial detection and enrichment system and an enrichment method thereof, which can realize automatic enrichment of samples to be tested, and can effectively avoid false positive or false negative caused by human factors. As a result, the detection is accurate.
  • the present invention adopts the following technical solutions:
  • An automatic microbial detection and enrichment system comprising:
  • a preset operation station for placing a sample to be tested, a culture medium containing the culture, a filter with a pipeline for collecting bacteria, and a filter plugging head; the filter is filtered through a membrane
  • the sample to be inspected is enriched with microorganisms that may be contained in the sample to be tested;
  • the sterilization chamber for sterilization After the preset operation station, the sterilization chamber for sterilization, the middle package removal tank for removing the middle package, the inner package removal chamber for removing the inner package, and the sample to be inspected for filtering and enriching microorganisms are sequentially disposed adjacently.
  • a conveying device for respectively feeding the sample to be inspected, the medium, the filter and the filter plugging head on the respective operating compartments, the conveying device starting from the preset operating station, running through the above-mentioned respective compartments ;
  • An industrial computer the industrial control electromechanical connection to each of the above compartments, each compartment isolation mechanism and the transmission device.
  • a sample to be tested, a medium containing the culture, and a filter are provided on the conveying device. Fixture for the plug and the filter plug; and/or,
  • the conveying device is divided into a sample conveying device to be inspected, a medium conveying device, a filter conveying device, a filter plugging head conveying device; and/or;
  • the conveying device is respectively provided with positioning sensors at predetermined positions in each of the compartments, and each positioning sensor is electrically connected to the industrial computer; and/or
  • the conveyor is a belt conveyor or a roller conveyor; the magnetic fluid in each compartment is driven by a motor disposed outside each compartment to drive a belt or a roller, or the belt is directly driven by a motor disposed in each compartment or Roller transfer; and/or,
  • Each of the compartment isolation mechanisms is an isolation valve.
  • each of the outer chambers is provided with a gas pressure adjusting device and a ventilation device for adjusting the pressures in the respective compartments; each of the air pressure adjusting devices and each of the air conditioning devices are electrically connected to the industrial computer;
  • the adjusting device includes a pressure sensor for sensing a pressure in the corresponding cabin, and each of the pressure sensors is electrically connected to the industrial computer;
  • a sterilization device for removing bacteria in the corresponding cabin is respectively disposed outside the sterilization chamber, the enrichment operation chamber and the buffer chamber.
  • the middle package removal compartment and the inner package removal compartment are respectively provided with a medium package removal mechanism and a medium for removing the filter and the filter package in the filter package and the inner package.
  • a package removing mechanism; the inner package removing mechanism and the middle package removing mechanism are electrically connected to the industrial computer respectively.
  • the filter with a pipeline is three tanks arranged in parallel at both ends, and the bottom of each tank has a filter membrane. After the sample to be inspected is injected, the microorganism is filtered.
  • Membrane enrichment the pipeline of the filter is three parallel pipelines, one end of the three parallel pipelines is disposed at the top end of the filter to respectively communicate with each tank body, and the other end of the three parallel pipelines a filter needle; the filter has a set position in the enrichment operation chamber, the set position is placed in the waste liquid tank support hole respectively at the bottom end of each of the can bodies;
  • An enrichment operation robot is disposed in the enrichment operation chamber above the conveying device, and the enrichment operation robot inserts the filter needle of the filter into the sample to be inspected and replaces the culture needle, and inserts the culture of the culture medium.
  • the enrichment operation robot can move the filter between the set position and the conveying device; the side of the enrichment operation robot near the buffer chamber is provided with one for driving the product to be inspected or the culture to inject the three An enriched drive mechanism that enters the filter in a parallel line;
  • a packaging mechanism for closing the bottom end of each can body through a filter plugging head after filtering and enriching is disposed in the enrichment operation cabin near the predetermined position;
  • the enrichment operation robot, the enrichment drive mechanism, and the packaging mechanism are electrically connected to the industrial computer respectively.
  • the enrichment drive mechanism is a peristaltic pump
  • the pump head of the peristaltic pump is provided with a first bracket and a second bracket, and the first bracket and the second bracket are in the enrichment operation.
  • the first bracket is telescopically fixed to the pump head of the peristaltic pump near the side of the enrichment robot
  • the second The bracket is telescopically disposed at a pump head of the peristaltic pump
  • the second bracket has a first position and a second position, the first position being disposed side by side at the first Next to the bracket, the three parallel pipelines are clamped together with the first bracket, and the second position is that the second bracket rotates to align the three parallel pipelines into the peristaltic pump.
  • the first bracket and the second branch respectively clamp the three parallel pipelines, and then rotate the second bracket to the second position, after the first bracket and the first bracket
  • the second bracket is retracted, and the three parallel tubes are placed in the peristaltic pump Head;
  • the enrichment operation robot further includes: when the filter completes the filtration enrichment of the sample to be inspected, the three parallel tubes can be sealed to at least two medium heat seals before the culture enters the filter. a mechanism whereby the culture can be selectively injected into a microbial enriched filter;
  • the triple operation pressure sensor is further provided with a triple pressure sensor for sensing the internal pressure of the filter;
  • the peristaltic pump, the first bracket, the second bracket, the medium heat sealing mechanism and the triple pressure sensor are electrically connected to the industrial computer respectively.
  • a buffer cutting mechanism is disposed in the buffer chamber above the conveying device;
  • the pipeline cutting mechanism is electrically connected to the industrial computer.
  • the positive bacteria filling chamber is a positive bacteria filling chamber with a heating function, and a positive bacteria filling mechanism is arranged above the conveying device in the positive bacteria filling chamber; There is also a temperature control device in the filling chamber;
  • the positive bacteria filling mechanism and the heating device are electrically connected to the industrial computer respectively; and/or
  • the industrial computer adopts the upper computer form, and each cabin can have a separate control unit.
  • a detection method using any of the above fully automated microbial detection and enrichment systems comprising the following steps:
  • the first step placing the sample to be inspected, the medium containing the culture, the filter with a pipeline dedicated to the bacteria collection, and the filter plugging head on the conveying device of the preset operation station;
  • Step 2 Open the cabin isolation mechanism between the preset operation station and the sterilization chamber, and pass the sample to be inspected, the culture medium containing the culture, the filter with the pipeline dedicated to the bacteria collection, and the filter plugging head.
  • the cabin isolation mechanism on both sides of the funnel is sterilized, and the pressure in the chamber is ventilated after sterilization is completed;
  • Step 3 Open the cabin isolation mechanism between the sterilization chamber and the medium package removal tank, and introduce the sample to be inspected, the medium containing the culture, the filter with the pipeline dedicated to the bacteria collection, and the filter plugging head.
  • the middle package is removed from the cabin, the middle compartment is closed to remove the cabin isolation mechanism on both sides of the tank, and the middle package of the filter and the filter plug is removed;
  • Step 4 Open the cabin isolation mechanism between the medium package removal compartment and the inner package removal compartment, and pass the sample to be inspected, the culture medium containing the culture, the special filter with the pipeline and the filter plugging head.
  • the inner package removal compartment close the inner compartment to remove the cabin isolation mechanism on both sides of the cabin, and remove the inner packaging of the filter and the filter plug;
  • Step 5 Open the cabin isolation mechanism between the inner packaging removal tank and the enrichment operation cabin, and pass the sample to be inspected, the culture medium containing the culture, the special filter with the pipeline and the filter plugging head Into the enriched operation chamber, the microorganisms in the sample to be tested are filtered and enriched in a filter, and then the bottom end of the filter is encapsulated by a filter plugging head, and then the culture is selectively injected into the filter to enrich the package.
  • a good filter is placed on the conveyor, and after obtaining a corresponding number of enriched and packaged filters, the cabin isolation mechanism on both sides of the enriched operation cabin is closed;
  • Step 6 Open the cabin isolation mechanism between the enriched operation cabin and the buffer tank, introduce the enriched and packaged filter into the buffer tank, and close the cabin isolation mechanism on both sides of the buffer tank to enrich The pipeline of the packaged filter is cut and cut;
  • the cabin isolation mechanism between the buffer chamber and the positive bacteria filling chamber is opened, and the filter that has been packaged after enrichment is introduced into the positive bacteria filling chamber, and the cabins on both sides of the positive bacteria filling chamber are closed.
  • the body isolation mechanism adds a positive bacteria to the filter which is packaged and enriched after enrichment, and completes the enrichment of microorganisms of the sample to be inspected.
  • the pressure of the previous compartment is increased each time the compartment isolation mechanism between the preset operation station, the sterilization chamber, the medium package removal compartment, the inner package removal compartment, and the enrichment operation compartment is opened.
  • Lower than the pressure of the latter tank to ensure that the bacteria are not brought into the enrichment operation cabin;
  • the cabin isolation mechanism between the enrichment operation cabin, the buffer tank and the positive bacteria filling chamber should be pressured in the previous cabin every time it is opened Higher than the pressure in the latter compartment, ensuring that the positive bacteria are not reversely transferred to the enriched operating compartment; and/or,
  • the method further comprises: a step of sterilizing the buffer tank; and/or,
  • the filter is a tank body having three outlets respectively arranged in parallel, and the culture can be selectively added to at most two tanks; and/or
  • the used sample to be tested and the culture medium are returned to the inner package removal tank through the conveying device, and are recovered in the inner package removal chamber.
  • the invention has the beneficial effects that the invention provides a fully automatic microbial detection and enrichment system and a method thereof, and fully enriches the microorganisms in the sample to be tested, without manual operation, saves time and labor, and can effectively avoid human factors.
  • Figure 1 is a top plan view of a preferred embodiment of the fully automated microbial detection and enrichment system of the present invention.
  • FIG. 2 is a front elevational view of a preferred embodiment of the fully automated microbial detection and enrichment system of the present invention.
  • FIG. 3 is a flow chart of a preferred embodiment of the fully automated microbial detection and enrichment method of the present invention.
  • the present invention provides a fully automatic microbial detection and enrichment system, which comprises:
  • a preset operation station 101 after which the preset sterilization operation chamber 101 is sequentially disposed adjacent to the sterilization sterilization chamber 102, the middle package removal medium compartment 103 for removing the middle package, and the inner package removal for removing the inner package a tank 104, injecting a sample to be inspected into a filter, enriching the microorganism, encapsulating the bottom end of the filter through a filter plugging head, and selectively entraining the culture into the filter, and enclosing the pipeline of the enriched filter
  • the cut-off buffer chamber 106 and the positive bacteria filling chamber 107 for positive bacteria are added.
  • Each of the above compartments is connected and closed by a cabin isolation mechanism 140.
  • the transmitting device 400 and the industrial computer 800 are connected and closed by a cabin isolation mechanism 140.
  • the preset operation station 101 is used for placing the sample to be tested 1000, the culture medium containing the culture, the filter 1100 with a pipeline dedicated to the bacteria collection, and the filter plugging head; the filter filters the sample to be inspected through the filter membrane. Enrich the microorganisms that may be contained in the sample to be tested.
  • the plugging heads are respectively fed into the conveying devices 400 of the respective tanks, and the conveying device 400 runs through the preset operating stations and penetrates the above respective cabins;
  • An industrial computer 800 is electrically connected to the above-mentioned respective cabins, the respective cabin isolation mechanisms 140 and the transmission device 400, and controls the respective cabins to perform corresponding operations.
  • the present invention can also provide a fully automated microbial enrichment method comprising the following steps:
  • the first step placing the sample to be inspected 1000, the culture medium containing the culture, the filter-equipped filter 1100 and the filter plugging head dedicated to the bacteria collection on the transfer device 400 of the preset operation station;
  • the second step opening the cabin isolation mechanism 140 between the preset operation station 101 and the sterilization chamber 102, and the sample 1000 to be inspected, the culture medium containing the culture, the special filter 1100 with the pipeline and the pipeline
  • the filter plugging head is introduced into the sterilization 102 chamber, and the cabin isolation mechanism on both sides of the sterilization chamber 102 is closed for sterilization, and the pressure in the gas exchange balance sterilization chamber 102 is completed after the sterilization is completed;
  • the third step opening the cabin isolation mechanism between the sterilization chamber 102 and the middle package removal chamber 103, and the sample 1000 to be inspected, the culture medium containing the culture, the special filter 1100 with the pipeline and the filter seal
  • the plug is introduced into the middle package removal compartment 103, and the cabin isolation mechanism 140 on both sides of the middle package removal compartment 103 is closed, and the medium package removal of the filter 1100 and the filter plug is performed;
  • the fourth step opening the cabin isolation mechanism 140 between the middle package removal compartment 103 and the inner package removal compartment 104, the sample 1000 to be inspected, the medium containing the culture, the special filter 1100 with the pipeline and the pipeline
  • the filter plugging head is introduced into the inner package to remove the cabin, and the inner compartment is removed from the cabin isolation mechanism on both sides of the tank, and the inner package of the filter 1100 and the filter plug is removed;
  • the fifth step opening the cabin isolation mechanism 140 between the inner package removal compartment 104 and the enrichment operation compartment 105, and the sample 1000 to be inspected, the culture medium containing the culture, the special filter 1100 with the pipeline and the pipeline
  • the filter plugging head is introduced into the enrichment operation chamber 105, and the microorganisms in the sample to be tested 1000 are filtered and enriched in the filter 1100, and then the bottom end of the filter is encapsulated by a filter plugging head, and then the culture is selectively selected.
  • the sixth step opening the cabin isolation mechanism 140 between the enrichment operation compartment 105 and the buffer compartment 106, introducing the enriched and packaged filter 1100 into the buffer compartment 106, and closing the cabins on both sides of the buffer compartment 105.
  • the isolation mechanism 140 cuts the pipeline of the enriched filter 1100;
  • the cabin isolation mechanism 140 between the buffer chamber 106 and the positive bacteria filling chamber 107 is opened, which will be rich.
  • the packaged filter 1100 is introduced into the positive bacteria filling chamber 107, and the cabin isolation mechanism 140 on both sides of the positive bacteria filling chamber 107 is closed, and the filter 1100 which is packaged and packaged after enrichment is filled with positive bacteria. The enrichment of microorganisms of the test sample is completed.
  • the present invention is applicable to the enrichment operation required for microbial limit safety detection in the field of food and drug safety, and is controlled by the industrial computer 800 throughout the entire process, thereby realizing automation and eliminating false positive or false negatives which may be caused by human factors. .
  • a fixing device for fixing a sample to be tested 1000, a culture medium containing the culture, a filter 1100, and a filter plugging head is provided on the conveying device 400. 150, thereby ensuring that the sample 1000 to be inspected, the culture medium containing the culture, the filter 1100 and the filter plugging head are not misplaced or dumped due to inertia during the transfer, thereby causing pollution in each tank and Affect the accuracy of each cabin operation.
  • the transfer device 400 is divided into a sample transfer device to be tested, a medium transfer device, a filter transfer device, and a filter plug transfer device to correspondingly transfer corresponding articles, reducing the complexity of the transfer device 400, such as in the buffer compartment 106 and Only the filter conveying device is needed in the positive bacteria filling chamber 107, without the need to send three additional conveying devices, saving space and reducing costs.
  • the transport device 400 is respectively provided with positioning sensors 200 at predetermined positions in each of the compartments, and each of the positioning sensors 200 is electrically connected to the industrial computer 800.
  • each positioning sensor 200 is an infrared sensor or an opposite sensor.
  • Each of the cabin isolation mechanisms 140 is an isolation valve.
  • the conveying device 400 is a belt conveying device or a roller conveying device; the magnetic fluid in each of the compartments is rotated by a motor disposed outside each compartment to drive the belt or the roller, or Drive the belt or roller directly through the motor set in each compartment;
  • each of the outer chambers is provided with a gas pressure adjusting device 110 and a ventilation device 130 for adjusting the pressures in the respective chambers; each of the air pressure adjusting devices 110 and Each of the ventilation devices 130 is electrically connected to the industrial computer; each of the air pressure adjustment devices 110 includes a pressure sensor for sensing a pressure in the corresponding cabin, and each of the pressure sensors is electrically connected to the industrial computer.
  • a sterilization device 120 for removing bacteria in the corresponding cabin is respectively disposed outside the sterilization chamber 102, the enrichment operation chamber 105, and the buffer chamber 106. The corresponding compartment can be sterilized to avoid contamination.
  • the preset operation station 101, the sterilization chamber 102, the middle package removal chamber 103, the inner package removal chamber 104, and the enrichment When the cabin isolation mechanism 140 between the operating compartments 105 is opened, the pressure of the previous compartment is lower than the pressure of the latter compartment, ensuring that bacteria are not carried into the enrichment operation cabin 105; the enrichment operation compartment 105, the buffer compartment 106 Each time the cabin isolation mechanism 140 between the positive bacteria filling chamber 107 is opened, the pressure of the previous tank is higher than the pressure of the latter tank, ensuring that the positive bacteria are not reversely transmitted to the enrichment operation cabin 106.
  • the method further includes a step of sterilizing the buffer chamber 106.
  • the buffer chamber 106 mainly serves to transmit and prevent positive bacteria from infecting each other. Because if the enrichment operating compartment 105 is directly connected to the positive bacteria filling chamber 107, the positive bacteria may enter the enrichment operating compartment 105.
  • the enriched operating cabin 105 is continuously operated, and wastes time if it is continuously sterilized.
  • Such a buffer compartment 106 can be connected to the enrichment operating compartment 105 after sterilization without interrupting the operation of the enriched operating compartment 105. When the buffer compartment 106 is isolated from the enrichment operating compartment 105, it can be in communication with the positive bacteria filling compartment 107.
  • the buffer chamber 106 can be sterilized, and then connected to the enrichment operation chamber 105 for the next filter 1100. Transfer. Thereby, the possibility that the positive bacteria in the positive bacteria filling chamber 107 enter the enrichment operation chamber 107 can be avoided, and the operation of the enrichment operation chamber 105 is not interrupted, so that the entire system has better continuous workability. high working efficiency.
  • the middle package removal compartment 103 and the inner package removal compartment 104 are respectively provided with a filter and a filter above the conveying device 400.
  • the middle package removing mechanism 310 and the inner package removing mechanism 320 of the middle and inner packages of the plugging head; the inner package removing mechanism 310 and the middle package removing mechanism 320 are electrically connected to the industrial computer respectively.
  • the filter 1100 with a pipeline is a tank body having three outlets respectively provided in parallel, preferably the tank body. Transparent.
  • the bottom of each tank has a filter membrane.
  • the pipeline of the filter 1100 is three parallel pipelines, and one end of the three parallel pipelines is disposed at the bottom.
  • the top end of the filter 1100 is respectively connected to each of the can bodies, and the other end of the three parallel pipes is a filter needle; the filter 1100 has a set position in the enrichment operation compartment 105, and the set position is each The bottom ends of the can bodies are respectively placed in the waste liquid tank support holes.
  • An enrichment operation robot 510 is disposed in the enrichment operation chamber 105 above the conveying device 400.
  • the enrichment operation robot 510 inserts the filter needle of the filter 1100 into the sample to be inspected and replaces the culture needle, and inserts the culture.
  • a culture of the base the enrichment operation robot 510 can cause the filter 1100 to be in the set position And moving between the conveyors; the side of the enrichment operation robot 510 near the buffer tank is provided with an enrichment for driving the product to be inspected or the culture into the three parallel pipelines into the filter 1100.
  • a packaging mechanism 530 is disposed in the enrichment operation chamber 105 near the predetermined position, and after filtering and enriching, the bottom end of each can is closed by a filter plugging head;
  • the enrichment operation robot 510, the enrichment drive mechanism 500, and the encapsulation mechanism 530 are electrically connected to the industrial computer 800, respectively, and are controlled by the industrial computer 800.
  • the enrichment drive mechanism 500 is a peristaltic pump
  • the pump head of the peristaltic pump is provided with a first bracket and a second bracket
  • the first bracket and the second bracket are The enrichment operation robot grips the filter needles into the sample to be inspected and clamps the three parallel tubes respectively.
  • the first bracket is telescopically fixed to the pump head of the peristaltic pump near the side of the enrichment robot 510
  • the second bracket is telescopically and rotatable around the pump head of the peristaltic pump. At the pump head.
  • the second bracket has a first position and a second position, the first position being disposed side by side of the first bracket for clamping the three parallel pipelines together with the first bracket, the second The position is that the second bracket is rotated to align the three parallel tubes to a position that can be placed in the pump head of the peristaltic pump.
  • the first bracket and the second branch respectively clamp the three parallel pipelines, and then rotate the second bracket to the second position, after which the first bracket and the second bracket are retracted, The three parallel tubes are placed in the pump head of the peristaltic pump.
  • the enrichment operation robot 510 further includes: when the filter completes the filtration enrichment of the sample to be inspected, the three parallel tubes can be sealed to at most two medium heats before the culture enters the filter. The mechanism is sealed so that the culture can be selectively injected into a filter that completes microbial enrichment. One or both of the triple lines may be selectively heat sealed or not sealed.
  • a triple pressure sensor 900 that senses the internal pressure of the filter 1100 is also disposed within the enrichment operating compartment 105, and the pressure within the operating compartment is enriched by sensing the pressure within the filter.
  • the peristaltic pump, the first bracket, the second bracket, the medium heat sealing mechanism and the triple pressure sensor 900 are electrically connected to the industrial computer 800, respectively.
  • the filter 1100 is placed at the set position by the enrichment robot 510, and then the filter needle is inserted into the sample to be seen 1000, and three parallel tubes are placed through the first bracket and the second bracket.
  • the peristaltic pump starts to work, and the liquid in the sample to be tested 1000 is filtered through the filter 1100, and It may contain microbes enriched in the filter membrane to complete the enrichment work.
  • the package mechanism 530 then closes the bottom end of each can through the filter plug to complete the package.
  • a pipeline is closed by the medium heat sealing mechanism, and then the enrichment robot 510 replaces the filter needle with the culture needle, inserts into the culture medium, and then injects the culture into the two pipelines by the action of the peristaltic pump. Enriched in the corresponding two tanks. In summary, enrichment of the filter, encapsulation, and injection of the culture are completed in the enrichment operating compartment 105.
  • the IPC executes internal control procedures based on the signals detected by the sensors to control the pressure in each compartment, the transmission of the conveyor, and the corresponding actions of the corresponding actuators in each compartment.
  • the control program is a common knowledge of those skilled in the art and is not invented by the inventors, and therefore will not be described herein.
  • the filter is three tanks arranged in parallel, and two of the tanks can be filled with the culture.
  • the used sample and medium to be tested are transferred back to the inner package removal tank through the conveying device 400, and recovered in the inner package removal chamber.
  • a buffer cutting mechanism 520 is disposed in the buffer chamber 106 above the conveying device 400 to encapsulate the filter. Pipeline.
  • the pipe cutting mechanism 520 is electrically connected to the industrial computer 800.
  • the positive bacteria filling chamber 107 is a positive bacteria filling chamber 107 having a heating function to fill the positive bacteria. After completion, the culture can be started in the positive bacteria filling chamber 107.
  • a positive bacteria filling mechanism 700 is disposed above the transfer device 400 in the positive bacteria filling chamber 107; a temperature control device is also provided in the positive bacteria filling chamber.
  • the positive bacteria filling mechanism 700 and the heating device are electrically connected to the industrial computer 800, respectively.
  • the industrial computer 800 is in the form of a host computer, and each cabin may have a separate control unit.
  • the present invention provides an automatic microbial detection and enrichment system and an enrichment method, which fully enriches microorganisms in a sample to be inspected without manual operation, saves time and labor, and can effectively avoid human factors.
  • the result of false positive or false negative caused by the influence realizes the aseptic automatic operation of the enrichment process, has the accurate effect of detection, is not easy to make mistakes during the enrichment process, and can work continuously during the enrichment operation without stopping After the enrichment operation, the enrichment operation cabin is suspended and sterilized, which has a high implementation effect.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Computer Hardware Design (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

提供一种全自动微生物检测富集系统及富集方法，所述系统和方法通过工控机控制传送装置将预置操作工位上的待检样品、含有培养物的培养基、集菌专用的带有管路的滤器和滤器封堵头，依次传入灭菌舱、中包装除去舱、内包装脱除舱、富集操作舱、缓冲舱、阳性菌加注舱中以进行相应的操作，对待检样品中的微生物进行全自动化富集。所述富集过程全程无均自动操作，检测准确，可连续工作，且可避免由于人为因素影响而造成的假阳性或假阴性的结果。

Description

全自动微生物检测富集系统及其富集方法 技术领域
本发明涉及微生物检测技术领域，尤指一种实现微生物检测的富集过程的自动化的全自动微生物检测富集系统及其富集方法。
背景技术
目前，微生物检测富集全部都是手工操作，消毒灭菌、集菌驱动、培养基加注及阳性菌加注各单元都是独立的，且阳性菌加注环境与集菌环境是不同的，即完成整个流程需要在不同的环境中完成，各个待检样品在不同环境间的传输依靠人工完成，极易受到人为因素的影响，极易产生假阳性或假阴性的结果。影响检测的准确性与时效性。
随着人们对食品和药品安全要求的提高，全自动无人为干预的微生物富集系统是食品和药品安全的必然需求。
发明内容
本发明的目的，在于提供一种全自动微生物检测富集系统及其富集方法，能够实现对待检样品进行自动化的富集，能有效避免由于人为因素的影响而造成的假阳性或假阴性的结果，检测准确。
为实现上述目的，本发明采用以下技术方案：
一种全自动微生物检测富集系统，它包含：
一预置操作工位，该预置操作工位用以放置的待检样品、含有培养物的培养基、集菌专用的带有管路的滤器和滤器封堵头；该滤器通过滤膜过滤待检样品，富集待检样品中可能含有的微生物；
在该预置操作工位后依次相邻地设置进行灭菌的灭菌舱、去除中包装的中包装除去舱、去除内包装的内包装脱除舱、将待检样品注入滤器过滤富集微生物和通过滤器封堵头将滤器底端封装以及选择性地将培养物注入滤器的富集操作舱、对富集完成的滤器的管路进行切装的缓冲舱、进行阳性菌加注的阳性菌加注舱；上述各舱分别通过舱体隔离机构相连与封闭；
一个用以将预置操作工位上的待检样品、培养基、滤器和滤器封堵头分别送入相应上述各舱的传送装置，该传送装置从预置操作工位开始，贯穿上述各舱；
一个工控机；该工控机电连接上述各舱、各舱体隔离机构和传送装置。
在一较佳实施例中，在传送装置上设有固定待检样品、含有培养物的培养基、滤 器和滤器封堵头的固定装置；和/或，
该传送装置分为待检样品传送装置、培养基传送装置、滤器传送装置、滤器封堵头传送装置；和/或；
该传送装置在各舱内的预定位置处分别设有定位传感器，各定位传感器分别电连接工控机；和/或，
该传送装置为皮带传送装置或滚轴传送装置；通过设置在各舱外的电机带动各舱内的磁流体转动，以驱动皮带或滚轴，或者通过设置在各舱内的电机直接驱动皮带或滚轴传送；和/或，
各该舱体隔离机构为隔离阀门。
在一较佳实施例中，所述各舱外分别设有调节各舱内压力的气压调节装置和通风装置；各该气压调节装置和各该通风装置分别电连接所述工控机；各该气压调节装置内包含有感测相应舱内压力的压力传感器，各该压力传感器电连接该工控机；和/或,
所述灭菌舱、富集操作舱、缓冲舱外分别设有一个灭除相应舱内细菌的灭菌装置。
在一较佳实施例中，所述中包装除去舱和所述内包装脱除舱在所述传送装置上方分别设有去除滤器和滤器封堵头中包装和内包装的中包装去除机构和内包装去除机构；该内包装去除机构和中包装去除机构分别电连接该工控机。
在一较佳实施例中，所述带有管路的滤器为三个并联设置的两端分别有出口的罐体，各个罐体的底部具有滤膜，注入待检样品后，微生物被该滤膜富集，该滤器的管路为三个并联的管路，该三个并联的管路的一端设置在所述滤器的顶端分别连通各该罐体，该三个并联的管路的另一端为一滤器针头；该滤器在所述富集操作舱内具有一设定位置，该设定位置为各所述罐体的底端分别放置于废液槽支撑孔中；
所述富集操作舱内在所述传送装置上方设有一个富集操作机械手，该富集操作机械手将所述滤器的滤器针头插入待检样品中以及更换培养物针头，插入培养基的培养物，该富集操作机械手可使该滤器在该设定位置以及传送装置间移动；该富集操作机械手靠近所述缓冲舱的一侧设有一个用以驱动待检验品或培养物注入所述三个并联的管路中进入所述滤器的富集驱动机构；
所述富集操作舱内靠近所述预定位置处设有过滤富集后通过滤器封堵头对各罐体底端进行封闭的封装机构；
该富集操作机械手、富集驱动机构和封装机构分别电连接该工控机。
在一较佳实施例中，所述富集驱动机构为一蠕动泵，该蠕动泵的泵头处设有第一支架和第二支架，该第一支架和该第二支架在该富集操作机械手夹持滤器针头插入所述待检样品中时分别夹持该三个并联的管路，第一支架可伸缩地固定在该蠕动泵的泵头靠近所述富集机械手一侧，该第二支架可伸缩地以及可绕该蠕动泵的泵头旋转的设置在该蠕动泵的泵头处，该第二支架具有一第一位置和一第二位置，该第一位置并排设置在该第一支架旁，用以同该第一支架一起夹持该该三个并联的管路，该第二位置为该第二支架旋转至将该三个并联的管路捋顺对应可放入该蠕动泵的泵头中的位置；在操作时，该第一支架和该第二支分别夹持该三个并联的管路，再将该第二支架旋转至第二位置，之后该第一支架和该第二支架后缩，将该三个并联的管路放入所述蠕动泵的泵头中；
所述富集操作机械手还包含一当所述滤器完成所述待检样品的过滤富集后，培养物进入所述滤器前可将该三个并联的管路封闭至多两个的培养基热封机构，如此可将培养物选择性的注入完成微生物富集的滤器中；
所述富集操作舱内还设有一个感测该滤器内部压力的三联压力传感器；
该蠕动泵、该第一支架、该第二支架、该培养基热封机构和该三联压力传感器分别电连接该工控机。
在一较佳实施例中，所述缓冲舱内在所述传送装置的上方设有一个管路切装机构；
该管路切装机构电连接所述工控机。
在一较佳实施例中，所述阳性菌加注舱为具有加热功能的阳性菌加注舱，在该阳性菌加注舱内的传送装置上方设有阳性菌加注机构；在该阳性菌加注舱内还设有温度控制装置；
该阳性菌加注机构和该加热装置分别电连接所述工控机；和/或，
该工控机采用上位机形式，各舱可具有单独控制单元。
一种应用上述任一全自动微生物检测富集系统的检测方法，它包含以下步骤：
第一步：将待检样品、含有培养物的培养基、集菌专用的带有管路的滤器和滤器封堵头放置在预置操作工位的所述传送装置上；
第二步：打开预置操作工位和灭菌舱之间的舱体隔离机构，将待检样品、含有培养物的培养基、集菌专用的带有管路的滤器和滤器封堵头传入所述灭菌舱内，关闭灭 菌舱两侧的舱体隔离机构，进行灭菌，灭菌完成后换气平衡灭菌舱内压力；
第三步：打开灭菌舱和中包装去除舱之间的舱体隔离机构，将待检样品、含有培养物的培养基、集菌专用的带有管路的滤器和滤器封堵头传入所述中包装去除舱中，关闭中包装去除舱两侧的舱体隔离机构，进行滤器和滤气器堵头的中包装去除；
第四步：打开中包装去除舱和内包装去除舱之间的舱体隔离机构，将待检样品、含有培养物的培养基、集菌专用的带有管路的滤器和滤器封堵头传入所述内包装去除舱中，关闭内包装去除舱两侧的舱体隔离机构，进行滤器和滤气器堵头的内包装去除；
第五步：打开内包装去除舱和富集操作舱之间的舱体隔离机构，将待检样品、含有培养物的培养基、集菌专用的带有管路的滤器和滤器封堵头传入所述富集操作舱中，将待检样品中的微生物过滤富集在滤器中，再通过滤器封堵头将滤器底端封装，之后选择性地将培养物注入滤器，将富集后封装好的滤器放置在传送装置上，获得相应数量的富集后封装好的滤器后，关闭富集操作舱两侧的舱体隔离机构；
第六步：打开富集操作舱和缓冲舱之间的舱体隔离机构，将富集后封装好的滤器传入所述缓冲舱中，关闭缓冲舱两侧的舱体隔离机构，对富集后封装好的滤器的管路进行切装；
第七步，打开缓冲舱和阳性菌加注舱之间的舱体隔离机构，将富集后封装好完成切装的滤器传入阳性菌加注舱，关闭阳性菌加注舱两侧的舱体隔离机构，对将富集后封装好完成切装的滤器加注阳性菌，完成被检样品的微生物的富集。
在一较佳实施例中，预置操作工位、灭菌舱、中包装除去舱、内包装脱除舱、富集操作舱之间的舱体隔离机构每一次开启时，前一个舱的压力低于后一个舱的压力，保证不将细菌带入富集操作舱；富集操作舱、缓冲舱和阳性菌加注舱之间的舱体隔离机构每一次开启时，前一个舱的压力要高于后一个舱的压力，保证不将阳性菌不逆向传递到富集操作舱中；和/或，
所述第七步中，关闭阳性菌加注舱两侧的舱体隔离机构后，还包含一步骤：对所述缓冲舱进行灭菌；和/或，
所述滤器为三个并联设置的两端分别有出口的罐体，可选择性地对其中至多两个罐体加注培养物；和/或，
在所述第五步中，用过的待检样品和培养基通过传送装置传回内包装去除舱，在内包装去除舱中回收。
本发明有益效果是：本发明提供一种全自动微生物检测富集系统及其方法，全自动化地对待检样品中的微生物进行富集，无需人工操作，省时省力，且能有效避免由于人为因素的影响而造成的假阳性或假阴性的结果，实现富集过程的无菌全自动操作，具有检测准确的实质效果，富集过程中不易出错，且在富集操作时可连续工作，无需在停止富集操作后对富集操作舱暂停杀菌，具有较高实施效果。
附图说明
图1是本发明的全自动微生物检测富集系统的一较佳实施例的俯视示意图。
图2是本发明的全自动微生物检测富集系统的一较佳实施例的主视示意图。
图3是本发明的全自动微生物检测富集方法的一较佳实施例的流程图。
附图标号：101：预置操作工位；102：灭菌舱；103：中包装除去舱；104：:内包装脱除舱；105：富集操作舱；106：缓冲舱；107：阳性菌加注舱；110：气压调节装置；120：灭菌装置；130：通风装置；200：定位传感器；310：中包装去除机构；320：内包装去除机构；140：舱体隔离机构；400：传送装置；500：富集驱动机构；510：富集操作机械手；520：管路切装机构；530：封装机构；700：阳性菌加注机构；800：工控机；900：三联压力传感器；1000：待检样品；1100：滤器。
具体实施方式
以下仅以实施例说明本发明可能的实施态样，然而并非用以限制本发明所欲保护的范畴，先予叙明。
图1、图2所示，本发明提供一种全自动微生物检测富集系统，它包含：
一个预置操作工位101，在该预置操作工位101后依次相邻地设置的进行灭菌的灭菌舱102、去除中包装的中包装除去舱103、去除内包装的内包装脱除舱104、将待检样品注入滤器过滤富集微生物和通过滤器封堵头将滤器底端封装以及选择性地将培养物注入滤器的富集操作舱105、对富集完成的滤器的管路进行切装的缓冲舱106、进行阳性菌加注的阳性菌加注舱107。上述各舱分别通过舱体隔离机构140相连与封闭。传送装置400和工控机800。
该预置操作工位101用以放置的待检样品1000、含有培养物的培养基、集菌专用的带有管路的滤器1100和滤器封堵头；该滤器通过滤膜过滤待检样品，富集待检样品中可能含有的微生物。
一个用以将预置操作工位101上的待检样品1000、培养基、滤器1100和滤器 封堵头分别送入相应上述各舱的传送装置400，该传送装置400从预置操作工位开始，贯穿上述各舱；
一个工控机800；该工控机800电连接上述各舱、各舱体隔离机构140和传送装置400，控制各舱进行相应的动作。
如图3所示，以此本发明还可以提供全自动微生物富集方法，它包含以下步骤：
第一步：将待检样品1000、含有培养物的培养基、集菌专用的带有管路的滤器1100和滤器封堵头放置在预置操作工位的所述传送装置400上；
第二步：打开预置操作工位101和灭菌舱102之间的舱体隔离机构140，将待检样品1000、含有培养物的培养基、集菌专用的带有管路的滤器1100和滤器封堵头传入所述灭菌102舱内，关闭灭菌舱102两侧的舱体隔离机构，进行灭菌，灭菌完成后换气平衡灭菌舱102内压力；
第三步：打开灭菌舱102和中包装去除舱103之间的舱体隔离机构，将待检样品1000、含有培养物的培养基、集菌专用的带有管路的滤器1100和滤器封堵头传入所述中包装去除舱103中，关闭中包装去除舱103两侧的舱体隔离机构140，进行滤器1100和滤气器堵头的中包装去除；
第四步：打开中包装去除舱103和内包装去除舱104之间的舱体隔离机构140，将待检样品1000、含有培养物的培养基、集菌专用的带有管路的滤器1100和滤器封堵头传入所述内包装去除舱中，关闭内包装去除舱两侧的舱体隔离机构，进行滤器1100和滤气器堵头的内包装去除；
第五步：打开内包装去除舱104和富集操作舱105之间的舱体隔离机构140，将待检样品1000、含有培养物的培养基、集菌专用的带有管路的滤器1100和滤器封堵头传入所述富集操作舱105中，将待检样品1000中的微生物过滤富集在滤器1100中，再通过滤器封堵头将滤器底端封装，之后选择性地将培养物注入滤器1100，将富集后封装好的滤器1100放置在传送装置上，获得相应数量的富集后封装好的滤器后，关闭富集操作舱105两侧的舱体隔离机构140；
第六步：打开富集操作舱105和缓冲舱106之间的舱体隔离机构140，将富集后封装好的滤器1100传入所述缓冲舱106中，关闭缓冲舱105两侧的舱体隔离机构140，对富集后的滤器1100的管路进行切装；
第七步，打开缓冲舱106和阳性菌加注舱107之间的舱体隔离机构140，将富 集后封装好的滤器1100传入阳性菌加注舱107，关闭阳性菌加注舱107两侧的舱体隔离机构140，对将富集后封装好完成切装的滤器1100加注阳性菌，完成被检样品的微生物的富集。
综上所述，本发明适用于目前食品与药品安全领域的微生物极限安全检测所需的富集操作，全程通过工控机800控制，实现了自动化，消除了人为因素可能产生的假阳性或假阴性。
在本发明的一较佳实施例中，如图1、图2所示，在传送装置400上设有固定待检样品1000、含有培养物的培养基、滤器1100和滤器封堵头的固定装置150，以此可保证待检样品1000、含有培养物的培养基、滤器1100和滤器封堵头在传输的过程中，不会因惯性的原因而错位或者倾倒，而造成各舱内的污染以及影响各舱操作时的准确性。
该传送装置400分为待检样品传送装置、培养基传送装置、滤器传送装置、滤器封堵头传送装置，以对应传送相应的物品，减小传送装置400的复杂性，例如在缓冲舱106和阳性菌加注舱107内只需要有滤器传送装置即可，而不需另外三送传送装置，节省空间，减小成本。
该传送装置400在各舱内的预定位置处分别设有定位传感器200，各定位传感器200分别电连接工控机800。以保证传送装置400传输时相应物品在各舱内的进行相应操作时位置的准确性，较佳地各定位传感器200为红外线传感器或对射传感器。
各该舱体隔离机构140为隔离阀门。
在本发明的一较佳实施例中，该传送装置400为皮带传送装置或滚轴传送装置；通过设置在各舱外的电机带动各舱内的磁流体转动，以驱动皮带或滚轴，或者通过设置在各舱内的电机直接驱动皮带或滚轴传送；。
在本发明的一较佳实施例中，如图1、图2所示，所述各舱外分别设有调节各舱内压力的气压调节装置110和通风装置130；各该气压调节装置110和各该通风装置130分别电连接所述工控机；各该气压调节装置110内包含有感测相应舱内压力的压力传感器，各该压力传感器电连接该工控机。以此可于舱与舱之间的传送时，通过气压调节装置110和通风装置130对各舱的压力进行控制。所述灭菌舱102、富集操作舱105、缓冲舱106外分别设有一个灭除相应舱内细菌的灭菌装置120。可对相应舱内进行灭菌，避免污染。
较佳地，在本较佳实施例中，本发明的全自动微生物富集方法中，预置操作工位101、灭菌舱102、中包装除去舱103、内包装脱除舱104、富集操作舱105之间的舱体隔离机构140每一次开启时，前一个舱的压力低于后一个舱的压力，保证不将细菌带入富集操作舱105；富集操作舱105、缓冲舱106和阳性菌加注舱107之间的舱体隔离机构140每一次开启时，前一个舱的压力要高于后一个舱的压力，保证不将阳性菌不逆向传递到富集操作舱106中。
且所述第七步中，关闭阳性菌加注舱106两侧的舱体隔离机构140后，还包含一步骤：对所述缓冲舱106进行灭菌。缓冲舱106主要是起传递、防止阳性菌相互感染的作用。因为如果富集操作舱105与阳性菌加注舱107直接相连，阳性菌可能进入富集操作舱105。而富集操作舱105是连续工作的，如果不断进行灭菌会浪费时间。如此缓冲舱106可以在灭菌后与富集操作舱105相连，不中断富集操作舱105的工作。当缓冲舱106与富集操作舱105隔离后，可与阳性菌加注舱107相连通。传输富集后封装好完成切装的滤器1100后进入阳性菌加注舱106后，即可隔离缓冲舱106进行灭菌，之后便可与富集操作舱105相连通，进行下一次的滤器1100转移。以此可避免阳性菌加注舱107内的阳性菌进入富集操作舱107的可能，且不中断富集操作仓105的操作，使整个系统具有较好的连续工作性。工作效率高。
如图1、图2所示，在本发明的一较佳实施例中，所述中包装除去舱103和所述内包装脱除舱104在所述传送装置400上方分别设有去除滤器和滤器封堵头的中包装和内包装的中包装去除机构310和内包装去除机构320；该内包装去除机构310和中包装去除机构320分别电连接该工控机。
在本发明的一较佳实施例中，如图1、图2所示，所述带有管路的滤器1100为三个并联设置的两端分别有出口的罐体，较佳地该罐体透明。各个罐体的底部具有滤膜，注入待检样品后，微生物被该滤膜富集，该滤器1100的管路为三个并联的管路，该三个并联的管路的一端设置在所述滤器1100的顶端分别连通各该罐体，该三个并联的管路的另一端为一滤器针头；该滤器1100在所述富集操作舱105内具有一设定位置，该设定位置为各所述罐体的底端分别放置于废液槽支撑孔中。
所述富集操作舱105内在所述传送装置400上方设有一个富集操作机械手510，该富集操作机械手510将所述滤器1100的滤器针头插入待检样品中以及更换培养物针头，插入培养基的培养物，该富集操作机械手510可使该滤器1100在该设定位置 以及传送装置间移动；该富集操作机械手510靠近所述缓冲舱的一侧设有一个用以驱动待检验品或培养物注入所述三个并联的管路中进入所述滤器1100的富集驱动机构500；
所述富集操作舱105内靠近所述预定位置处设有过滤富集后通过滤器封堵头对各罐体底端进行封闭的封装机构530；
该富集操作机械手510、富集驱动机构500和封装机构530分别电连接该工控机800，被该工控机800控制。
在本发明的一较佳实施例中，所述富集驱动机构500为一蠕动泵，该蠕动泵的泵头处设有第一支架和第二支架，该第一支架和该第二支架在该富集操作机械手夹持滤器针头插入所述待检样品中时分别夹持该三个并联的管路。其中，第一支架可伸缩地固定在该蠕动泵的泵头靠近所述富集机械手510一侧，该第二支架可伸缩地以及可绕该蠕动泵的泵头旋转的设置在该蠕动泵的泵头处。该第二支架具有一第一位置和一第二位置，该第一位置并排设置在该第一支架旁，用以同该第一支架一起夹持该该三个并联的管路，该第二位置为该第二支架旋转至将该三个并联的管路捋顺对应可放入该蠕动泵的泵头中的位置。在操作时，该第一支架和该第二支分别夹持该三个并联的管路，再将该第二支架旋转至第二位置，之后该第一支架和该第二支架后缩，将该三个并联的管路放入所述蠕动泵的泵头中。
所述富集操作机械手510还包含一当所述滤器完成所述待检样品的过滤富集后，培养物进入所述滤器前可将该三个并联的管路封闭至多两个的培养基热封机构，如此可将培养物选择性地注入完成微生物富集的滤器中。可以选择性地热封三联管路中的一个或者两个或者都不密封。
所述富集操作舱105内还设有一个感测该滤器1100内部压力的三联压力传感器900，通过感测滤器内的压力调节富集操作舱内的压力。
该蠕动泵、该第一支架、该第二支架、该培养基热封机构和该三联压力传感器900分别电连接该工控机800。
以此，在所述富集操作舱105内进行操作时，为以下动作：
通过富集机械手510将所述滤器1100放置在该设定位置处，之后再将过滤器针头插入待见样品1000中，在通过第一支架和第二支架，将三个并联的管路放入蠕动泵的泵头内，蠕动泵开始工作，将待检样品1000中的液体通过滤器1100过滤，将 可能含有的微生物富集滤膜上，完成富集工作。之后封装机构530通过滤器封堵头封闭各个罐体的底端，以完成封装。再通过培养基热封机构选封闭一个管路，之后富集机械手510将滤器针头更换为培养物针头，插入培养基中培养物内，再通过蠕动泵的作用将培养物注入两个管路，富集在相对应的两个罐体中。综上，在该富集操作舱105内完成滤器的富集、封装与培养物的注入。
工控机根据各传感器检测的信号执行内部的控制程序，分别控制各舱内的压力、传送装置的传输及各舱内相应的执行装置的相应动作。该控制程序为本领域技术人员的公知常识，不是本发明人发明创造的，故在此不在赘述。
以此在本发明的全自动微生物富集方法的一较佳实施例中，所述滤器为三个并联设置的罐体，可对其中的两个罐体加注培养物。
较佳地在所述第五步中，用过的待检样品和培养基通过传送装置400传回内包装去除舱，在内包装去除舱中回收。
较佳地，如图1、图2所示，本发明的一较佳实施例中，所述缓冲舱106内在所述传送装置400的上方设有一个管路切装机构520，以封装该滤器的管路。
该管路切装机构520电连接所述工控机800。
较佳地，如图1、图2所示，本发明的一较佳实施例中，所述阳性菌加注舱107为具有加热功能的阳性菌加注舱107，以在该阳性菌加注完成后在该阳性菌加注舱107内即可开始培养。在该阳性菌加注舱107内的传送装置400上方设有阳性菌加注机构700；在该阳性菌加注舱内还设有温度控制装置。
该阳性菌加注机构700和该加热装置分别电连接所述工控机800。
较佳地，该工控机800采用上位机形式，各舱可具有单独控制单元。
综上所述，本发明提供一种全自动微生物检测富集系统及富集方法，全自动化地对待检样品中的微生物进行富集，无需人工操作，省时省力，且能有效避免由于人为因素的影响而造成的假阳性或假阴性的结果，实现富集过程的无菌全自动操作，具有检测准确的实质效果，富集过程中不易出错，且在富集操作时可连续工作，无需停止富集操作后对富集操作舱暂停杀菌，具有较高实施效果。

Claims (10)

  1. 一种全自动微生物检测富集系统，其特征在于，它包含：
    一预置操作工位，该预置操作工位用以放置的待检样品、含有培养物的培养基、集菌专用的带有管路的滤器和滤器封堵头；该滤器通过滤膜过滤待检样品，富集待检样品中可能含有的微生物；
    在该预置操作工位后依次相邻地设置进行灭菌的灭菌舱、去除中包装的中包装除去舱、去除内包装的内包装脱除舱、将待检样品注入滤器过滤富集微生物和通过滤器封堵头将滤器底端封装以及选择性地将培养物注入滤器的富集操作舱、对富集完成的滤器的管路进行切装的缓冲舱、进行阳性菌加注的阳性菌加注舱；上述各舱分别通过舱体隔离机构相连与封闭；
    一个用以将预置操作工位上的待检样品、培养基、滤器和滤器封堵头分别送入相应上述各舱的传送装置，该传送装置从预置操作工位开始，贯穿上述各舱；
    一个工控机；该工控机电连接上述各舱、各舱体隔离机构和传送装置。
  2. 根据权利要求1所述的全自动微生物检测富集系统，其特征在于，在传送装置上设有固定待检样品、含有培养物的培养基、滤器和滤器封堵头的固定装置；和/或，
    该传送装置分为待检样品传送装置、培养基传送装置、滤器传送装置、滤器封堵头传送装置；和/或；
    该传送装置在各舱内的预定位置处分别设有定位传感器，各定位传感器分别电连接工控机；和/或，
    该传送装置为皮带传送装置或滚轴传送装置；通过设置在各舱外的电机带动各舱内的磁流体转动，以驱动皮带或滚轴，或者通过设置在各舱内的电机直接驱动皮带或滚轴传送；和/或，
    各该舱体隔离机构为隔离阀门。
  3. 根据权利要求1所述的全自动微生物检测富集系统，其特征在于，所述各舱外分别设有调节各舱内压力的气压调节装置和通风装置；各该气压调节装置和各该通风装置分别电连接所述工控机；各该气压调节装置内包含有感测相应舱内压力的压力传感器，各该压力传感器电连接该工控机；和/或,
    所述灭菌舱、富集操作舱、缓冲舱外分别设有一个灭除相应舱内细菌的灭菌装置。
  4. 根据权利要求1所述的全自动微生物检测富集系统，其特征在于，所述中包装除去舱和所述内包装脱除舱在所述传送装置上方分别设有去除滤器和滤器封堵头中包装和内包装的中包装去除机构和内包装去除机构；该内包装去除机构和中包装去除机构分别电连接该工控机。
  5. 根据权利要求1所述的全自动微生物检测富集系统，其特征在于，所述带有管路的滤器为三个并联设置的两端分别有出口的罐体，各个罐体的底部具有滤膜，注入待检样品后，微生物被该滤膜富集，该滤器的管路为三个并联的管路，该三个并联的管路的一端设置在所述滤器的顶端分别连通各该罐体，该三个并联的管路的另一端为一滤器针头；该滤器在所述富集操作舱内具有一设定位置，该设定位置为各所述罐体的底端分别放置于废液槽支撑孔中；
    所述富集操作舱内在所述传送装置上方设有一个富集操作机械手，该富集操作机械手将所述滤器的滤器针头插入待检样品中以及更换培养物针头，插入培养基的培养物，该富集操作机械手可使该滤器在该设定位置以及传送装置间移动；该富集操作机械手靠近所述缓冲舱的一侧设有一个用以驱动待检验品或培养物注入所述三个并联的管路中进入所述滤器的富集驱动机构；
    所述富集操作舱内靠近所述预定位置处设有过滤富集后通过滤器封堵头对各罐体底端进行封闭的封装机构；
    该富集操作机械手、富集驱动机构和封装机构分别电连接该工控机。
  6. 根据权利要求6所述的全自动微生物检测富集系统，其特征在于，所述富集驱动机构为一蠕动泵，该蠕动泵的泵头处设有第一支架和第二支架，该第一支架和该第二支架在该富集操作机械手夹持滤器针头插入所述待检样品中时分别夹持该三个并联的管路，第一支架可伸缩地固定在该蠕动泵的泵头靠近所述富集机械手一侧，该第二支架可伸缩地以及可绕该蠕动泵的泵头旋转的设置在该蠕动泵的泵头处，该第二支架具有一第一位置和一第二位置，该第一位置并排设置在该第一支架旁，用以同该第一支架一起夹持该该三个并联的管路，该第二位置为该第二支架旋转至将该三个并联的管路捋顺对应可放入该蠕动泵的泵头中的位置；在操作时，该第一支架和该第二支分别夹持该三个并联的管路，再将该第二支架旋转至第二位置，之后该第一支架和该第二支架后缩，将该三个并联的管路放入所述蠕动泵的泵头中；
    所述富集操作机械手还包含一当所述滤器完成所述待检样品的过滤富集后，培养物进入所述滤器前可将该三个并联的管路封闭至多两个的培养基热封机构，如此可将培养物选择性的注入完成微生物富集的滤器中；
    所述富集操作舱内还设有一个感测该滤器内部压力的三联压力传感器；
    该蠕动泵、该第一支架、该第二支架、该培养基热封机构和该三联压力传感器分别电连接该工控机。
  7. 根据权利要求1所述的全自动微生物检测富集系统，其特征在于，所述缓冲舱内在所述传送装置的上方设有一个管路切装机构；
    该管路切装机构电连接所述工控机。
  8. 根据权利要求1所述的全自动微生物检测富集系统，其特征在于，所述阳性菌加注舱为具有加热功能的阳性菌加注舱，在该阳性菌加注舱内的传送装置上方设有阳性菌加注机构；在该阳性菌加注舱内还设有温度控制装置；
    该阳性菌加注机构和该加热装置分别电连接所述工控机；和/或，
    该工控机采用上位机形式，各舱可具有单独控制单元。
  9. 根据上述任一权利要求所述的全自动微生物富集方法，其特征在于，它包含以下步骤：
    第一步：将待检样品、含有培养物的培养基、集菌专用的带有管路的滤器和滤器封堵头放置在预置操作工位的所述传送装置上；
    第二步：打开预置操作工位和灭菌舱之间的舱体隔离机构，将待检样品、含有培养物的培养基、集菌专用的带有管路的滤器和滤器封堵头传入所述灭菌舱内，关闭灭菌舱两侧的舱体隔离机构，进行灭菌，灭菌完成后换气平衡灭菌舱内压力；
    第三步：打开灭菌舱和中包装去除舱之间的舱体隔离机构，将待检样品、含有培养物的培养基、集菌专用的带有管路的滤器和滤器封堵头传入所述中包装去除舱中，关闭中包装去除舱两侧的舱体隔离机构，进行滤器和滤气器堵头的中包装去除；
    第四步：打开中包装去除舱和内包装去除舱之间的舱体隔离机构，将待检样品、含有培养物的培养基、集菌专用的带有管路的滤器和滤器封堵头传入所述内包装去除舱中，关闭内包装去除舱两侧的舱体隔离机构，进行滤器和滤气器堵头的内包装去除；
    第五步：打开内包装去除舱和富集操作舱之间的舱体隔离机构，将待检样品、含有培养物的培养基、集菌专用的带有管路的滤器和滤器封堵头传入所述富集操作舱 中，将待检样品中的微生物过滤富集在滤器中，再通过滤器封堵头将滤器底端封装，之后选择性地将培养物注入滤器，将富集后封装好的滤器放置在传送装置上，获得相应数量的富集后封装好的滤器后，关闭富集操作舱两侧的舱体隔离机构；
    第六步：打开富集操作舱和缓冲舱之间的舱体隔离机构，将富集后封装好的滤器传入所述缓冲舱中，关闭缓冲舱两侧的舱体隔离机构，对富集后封装好的滤器的管路进行切装；
    第七步，打开缓冲舱和阳性菌加注舱之间的舱体隔离机构，将富集后封装好完成切装的滤器传入阳性菌加注舱，关闭阳性菌加注舱两侧的舱体隔离机构，对将富集后封装好完成切装的滤器加注阳性菌，完成被检样品的微生物的检测富集。
  10. 根据权利要求10所述的全自动微生物富集方法，其特征在于，预置操作工位、灭菌舱、中包装除去舱、内包装脱除舱、富集操作舱之间的舱体隔离机构每一次开启时，前一个舱的压力低于后一个舱的压力，保证不将细菌带入富集操作舱；富集操作舱、缓冲舱和阳性菌加注舱之间的舱体隔离机构每一次开启时，前一个舱的压力要高于后一个舱的压力，保证不将阳性菌不逆向传递到富集操作舱中；和/或，
    所述第七步中，关闭阳性菌加注舱两侧的舱体隔离机构后，还包含一步骤：对所述缓冲舱进行灭菌；和/或，
    所述滤器为三个并联设置的两端分别有出口的罐体，可选择性地对其中至多两个罐体加注培养物；和/或，
    在所述第五步中，用过的待检样品和培养基通过传送装置传回内包装去除舱，在内包装去除舱中回收。
PCT/CN2014/095848 2013-12-31 2014-12-31 全自动微生物检测富集系统及其富集方法 WO2015101326A1 (zh)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP14877171.0A EP3091068B1 (en) 2013-12-31 2014-12-31 Full-automatic microbial detection and enrichment system and enrichment method thereof
CA2935299A CA2935299C (en) 2013-12-31 2014-12-31 Full-automatic microorganism detecting enrichment system and enrichment method thereof
AU2014375463A AU2014375463B2 (en) 2013-12-31 2014-12-31 Full-automatic microbial detection and enrichment system and enrichment method thereof
JP2016561066A JP2017502696A (ja) 2013-12-31 2014-12-31 全自動式微生物検出用集積システム及びその集積方法
US15/106,930 US20170029760A1 (en) 2013-12-31 2014-12-31 Full-automatic microorganism detecting enrichment system and enrichment method thereof
KR1020167020524A KR101807984B1 (ko) 2013-12-31 2014-12-31 전자동 미생물 검출 농축 시스템 및 그 농축 방법

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201310751864.2 2013-12-31
CN201310751864.2A CN104745464B (zh) 2013-12-31 2013-12-31 全自动微生物检测富集系统及其富集方法

Publications (1)

Publication Number Publication Date
WO2015101326A1 true WO2015101326A1 (zh) 2015-07-09

Family

ID=53493267

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2014/095848 WO2015101326A1 (zh) 2013-12-31 2014-12-31 全自动微生物检测富集系统及其富集方法

Country Status (8)

Country Link
US (1) US20170029760A1 (zh)
EP (1) EP3091068B1 (zh)
JP (1) JP2017502696A (zh)
KR (1) KR101807984B1 (zh)
CN (1) CN104745464B (zh)
AU (1) AU2014375463B2 (zh)
CA (1) CA2935299C (zh)
WO (1) WO2015101326A1 (zh)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10897922B2 (en) 2016-04-05 2021-01-26 Nch Corporation Composition and method for germinative compounds in probiotic food and beverage products for human consumption
CN106010955B (zh) * 2016-07-22 2018-12-11 牛刚 无菌舱药品无菌检查富集作业车
CN106226151B (zh) * 2016-07-22 2019-03-08 牛刚 无菌舱全自动药品无菌检查富集系统及其操作方法
CN107505439A (zh) * 2017-07-27 2017-12-22 马鹏飞 一种基于机器人运动技术的药品无菌检测仪
US11155780B2 (en) 2017-10-04 2021-10-26 NCH Life Sciences LLC Metastable state mixing
CN108426941A (zh) * 2017-12-31 2018-08-21 宁波华仪宁创智能科技有限公司 一种安检仪及方法
CN107988069A (zh) * 2018-01-30 2018-05-04 杨军 一种医疗用微生物检验检疫用培养装置
CN108611264A (zh) * 2018-05-10 2018-10-02 朱丹 一种多批次生物制品共线制备的系统及方法
CN109266521A (zh) * 2018-09-18 2019-01-25 江门出入境检验检疫局检验检疫技术中心 气动式多通道微生物培养基自动制备分装仪
CN109459410A (zh) * 2018-11-21 2019-03-12 郑州博莱特生物科技有限公司 食品微生物自动快速检测系统
CN109795857A (zh) * 2019-02-19 2019-05-24 浙江大学 隔离器集菌操作传输系统
CN110734845B (zh) * 2019-11-22 2023-11-24 中国科学院海洋研究所 基于rov的深海极端环境微生物初级生产力原位检测装置及方法
KR102383013B1 (ko) * 2020-03-23 2022-04-05 프레스티지바이오로직스 주식회사 항체 의약품 제조 공정을 위한 제균 필터 시스템 및 그 작동 방법
CN112226348B (zh) * 2020-10-26 2023-06-16 达尔文实验机器人成都有限公司 应用生物医药注射剂微生物检测机器人系统的集菌方法
CN113138109B (zh) * 2021-04-23 2022-07-01 贵州省产品质量检验检测院 一种旋转重复式样品中微量组分提取富集装置
CN113549540B (zh) * 2021-06-29 2024-05-31 杭州电子科技大学 一种智能化集菌系统
CN113549534B (zh) * 2021-06-29 2024-05-31 杭州电子科技大学 一种智能集菌操作系统的控制输液及集菌装置
CN113897274B (zh) * 2021-10-21 2022-05-06 九江学院 一种微生物富集培养装置
CN114456919B (zh) * 2022-03-17 2022-09-16 南方海洋科学与工程广东省实验室(广州) 一种高压环境海洋微生物固体分离培养装置及培养方法
CN114350507A (zh) * 2022-03-17 2022-04-15 广东工业大学 一种深海原位环境的单菌落分离装置及分离方法
CN114350508B (zh) * 2022-03-17 2022-07-12 南方海洋科学与工程广东省实验室(广州) 高压环境海洋微生物富集培养与重力式分离装置
CN114350509B (zh) * 2022-03-17 2022-11-04 南方海洋科学与工程广东省实验室(广州) 高压环境生物富集与喷洒式固体分离培养装置
CN116273251B (zh) * 2023-02-14 2023-08-25 北京赛赋医药研究院有限公司 一种用于干细胞制备的无菌操作装置及其使用方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1285878A (zh) * 1997-11-03 2001-02-28 埃利亚斯·哈卡莱托 用于富集和寻找微生物样品的方法和装置
CN201756550U (zh) * 2010-06-29 2011-03-09 中国人民解放军第三○二医院 一种全封闭集菌安瓿培养器
CN203833938U (zh) * 2013-12-31 2014-09-17 牛刚 全自动微生物检测富集系统

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2515317B2 (ja) * 1987-03-17 1996-07-10 武田薬品工業株式会社 バイアルの自動無菌試験装置
JPH07274939A (ja) * 1994-04-06 1995-10-24 Umetani Seiki:Kk 自動無菌試験装置
AR035231A1 (es) * 2002-03-11 2004-05-05 Ypf S A Un equipo para analizar el crecimiento de microorganismos y procedimiento para cuantificar la concentracion de microorganismos
JP4329066B2 (ja) * 2003-08-22 2009-09-09 澁谷工業株式会社 無菌試験サンプリング方法及びその装置
EP2392643B1 (en) * 2009-01-29 2016-10-26 Hitachi High-Technologies Corporation Device and method for automatically analyzing bacteria and fungi
CN201681012U (zh) * 2010-04-27 2010-12-22 天津市欧诺仪器仪表有限公司 环境水样快速病毒富集装置
CN201793573U (zh) * 2010-09-08 2011-04-13 牛刚 压强检测装置及带有压强检测装置的微生物检验薄膜系统
CN201883098U (zh) * 2010-12-01 2011-06-29 汪华 一种医用细胞自动化生产装置
CN102174395B (zh) * 2011-01-30 2013-08-21 中国科学院广州生物医药与健康研究院 诱导多能干细胞自动化扩增与培养系统
CN202956299U (zh) * 2012-12-03 2013-05-29 复旦大学 便携式的生物气溶胶富集及快速检测分析装置

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1285878A (zh) * 1997-11-03 2001-02-28 埃利亚斯·哈卡莱托 用于富集和寻找微生物样品的方法和装置
CN201756550U (zh) * 2010-06-29 2011-03-09 中国人民解放军第三○二医院 一种全封闭集菌安瓿培养器
CN203833938U (zh) * 2013-12-31 2014-09-17 牛刚 全自动微生物检测富集系统

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP3091068A4 *

Also Published As

Publication number Publication date
CN104745464A (zh) 2015-07-01
EP3091068B1 (en) 2019-01-09
CN104745464B (zh) 2016-09-14
CA2935299A1 (en) 2015-07-09
EP3091068A4 (en) 2017-10-18
KR20160104029A (ko) 2016-09-02
EP3091068A1 (en) 2016-11-09
US20170029760A1 (en) 2017-02-02
AU2014375463A1 (en) 2016-07-07
JP2017502696A (ja) 2017-01-26
CA2935299C (en) 2019-01-22
KR101807984B1 (ko) 2017-12-11
AU2014375463B2 (en) 2017-05-25

Similar Documents

Publication Publication Date Title
WO2015101326A1 (zh) 全自动微生物检测富集系统及其富集方法
CN103083788B (zh) 输液管自动生产线
JP7043115B2 (ja) 細胞培養用バッグアセンブリ
US20150153257A1 (en) Sample preparation device
CN107187638A (zh) 真空包装机真空系统及其控制方法
CN207045834U (zh) 真空包装机真空系统
CN106226151B (zh) 无菌舱全自动药品无菌检查富集系统及其操作方法
US20230203419A1 (en) Bioprocessing System
CN106141663A (zh) 一种输液器装配工艺
CN106834421B (zh) 面向西林瓶装粉末供试品的全自动化流水线集菌操作方法和系统
CN103250569B (zh) 一种自动间歇式食用菌液体菌种接种系统
CN203833938U (zh) 全自动微生物检测富集系统
CN106834422B (zh) 面向安瓿瓶装水溶液供试品的全自动化流水线集菌操作方法和系统
CN116445272A (zh) 一种自动化集菌系统
CN215649082U (zh) 一种粉剂生产用杀菌装置
WO2021047634A1 (zh) 一种一次性取样装置
CN211013944U (zh) 一种空气过滤器的在线检测装置
CN208742441U (zh) 一种温控翻转振荡器
CN113549534A (zh) 一种智能集菌操作系统的控制输液及集菌装置
CN214407926U (zh) 一种透析器生产用自动测漏输送线
CN205426558U (zh) 一种无菌检查取样装置
CN210560374U (zh) 可多次取样的低成本厌氧发酵装置
CN220018862U (zh) 白细胞过滤器气密性批量检测系统
CN212532998U (zh) 一种微生物采样装置
CN220595425U (zh) 一种培养基无菌灌装生产线

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 14877171

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 15106930

Country of ref document: US

ENP Entry into the national phase

Ref document number: 2935299

Country of ref document: CA

Ref document number: 2016561066

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 2014375463

Country of ref document: AU

Date of ref document: 20141231

Kind code of ref document: A

ENP Entry into the national phase

Ref document number: 20167020524

Country of ref document: KR

Kind code of ref document: A

REEP Request for entry into the european phase

Ref document number: 2014877171

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2014877171

Country of ref document: EP