WO2015099616A1 - Mélange permettant de réduire la masse corporelle - Google Patents

Mélange permettant de réduire la masse corporelle Download PDF

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Publication number
WO2015099616A1
WO2015099616A1 PCT/SI2014/000067 SI2014000067W WO2015099616A1 WO 2015099616 A1 WO2015099616 A1 WO 2015099616A1 SI 2014000067 W SI2014000067 W SI 2014000067W WO 2015099616 A1 WO2015099616 A1 WO 2015099616A1
Authority
WO
WIPO (PCT)
Prior art keywords
extract
mixture
weight
mixture according
body mass
Prior art date
Application number
PCT/SI2014/000067
Other languages
English (en)
Inventor
Majda Hadolin Kolar
Karmen TESKAC PLAJNŠEK
Dušanka DIMITRIJEVIC
Thierry Gay
Ohad Ovadya COHEN
Original Assignee
Vitiva Proizvodnja In Storitve D.D.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from SI201300442A external-priority patent/SI24563A/sl
Application filed by Vitiva Proizvodnja In Storitve D.D. filed Critical Vitiva Proizvodnja In Storitve D.D.
Publication of WO2015099616A1 publication Critical patent/WO2015099616A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/74—Rubiaceae (Madder family)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/30—Dietetic or nutritional methods, e.g. for losing weight
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/41—Crassulaceae (Stonecrop family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/82—Theaceae (Tea family), e.g. camellia

Definitions

  • the object of the invention is a mixture used as a dietary supplement for reducing body mass.
  • the mixture includes six plant extracts: rosemary leaf extract (Rosmarinus officinalis), green coffee extract (Coffea Camphor a robusta P.), black radish root extract (Raphanus sativus), green tea leaf extract (Camelia sinensis), cinnamon bark extract (Cinnamonum cassia Presl) and rose root extract (Rhodiola rosea L.).
  • the mixture not only contributes to the reduction of body mass but also contributes to a reduced waist size, improved cardiovascular parameters, such as blood pressure reduction, heart beat regulation, blood lipid and glucose reduction as well as to improved personal aspects, such as improved mood, better sleep, reduced feeling of hunger and appetite. ⁇
  • the task and goal of the invention is therefore to prepare a mixture that will be used as a dietary supplement for reducing body mass and will include various mechanisms of regulating body mass such as:
  • the task of the invention is solved by a mixture including six plant extracts: rosemary leaf extract (Rosmarinus officinalis), green coffee extract (Coffea Canephora robusta P.), black radish root extract (Raphanus sativus), green tea leaf extract (Camelia sinensis), cinnamon bark extract (Cinnamonum cassia Presl) and rose root extract (Rhodiola rosea L.).
  • the extracts are standardized to following active ingredients: 1.
  • the rosemary extract contains at least 15 % by weight of rosemarinic acid.
  • the green coffe extract contains at least 45 % by weight of chloro genie acids.
  • the green tea leaf extract contains at least 95 % by weight of polyphenols, of that at least 40 % by weight of epigallocatechin gallate.
  • the cinnamon bark extract contains at least 20 % by weight of polyphenols.
  • the rose root extract contains at least 1 % by weight of salidrosides and at least 3 % by weight of rosavines.
  • the black radish root extract is not standardized.
  • the mixture contains 10-60 % of green coffee extract, 5-40 % of green tea extract, 0.5-20 % of rose root extract, 10-60 % of rosemary extract, 1-20 % of cinnamon bark extract and 1- 20 % of black radish extract.
  • the indicated percents are the percents by weight.
  • the content of active ingredients in the mixture is standardized in a way that a daily dosage of the mixture contains 100-300 mg of chlorogenic acids, 40-120 mg of total polyphenols, of that 20-60 mg of epigallocatechin gallate, up to 2 mg of salidroside and 6 mg of rosavines, 10-150 mg of rosemarinic acid, 2-30 mg of cinnamon polyphenols and 20-30 mg of black radish extract.
  • a daily dosage of the mixture preferably contains 380 mg of green coffee extract which corresponds to 170 mg of chlorogenic acids; 100 mg of green tea leaf extract which corresponds to 95 mg of total polyphenols, of that 40 mg of epigallocatechin gallate; 20 mg of rose root extract which corresponds to 0.2 mg of salidrosides and 0.6 mg of rosavines; 400 mg of rosemary leaf extract which corresponds to 60 mg of rosemarinic acid; 50 mg of cinnamon bark extract which corresponds to 10 mg of polyphenols, and 50 mg of black radish extract.
  • a daily dosage is divided into a daily capsule containing the extracts of green coffee, green tea leaves and rose root, and a night capsule containing the extracts of rosemary leaves, cinnamon bark and black radish root.
  • the daily and night capsules are composed based on the mechanism of activity.
  • a daily capsule reduces appetite, reduces glucose absorption and accumulation of glucose in fat cells, improves metabolism and has antistress activity.
  • the antistress component is added due to a reduced intake of calories while a person is losing weight and a human organism can react by stress.
  • a night capsule accelerates detoxification of an organism, inhibits the activity of absorption enzymes and increases thermogenesis (fat burning effect).
  • cinnamon bark extract increases thermogenesis, it has a fat burning effect
  • rose root extract has antistress activity.
  • Figure 1 Positive effects of taking the extract mixture in comparison with placebo in women.
  • Figure 2 Positive effects of taking the extract mixture in comparison with placebo in men.
  • Table 1 Composition and features of a mixture for body weight regulation.
  • the prepared mixture was tested »in vitro « as to its capability of inhibiting differentiation of fat cells (adipocytes), wherein human fat cells were used as a model (the assay was performed in the BlOalternatives Laboratory, France, study number: AX11 1202, august 2012).
  • the mixture for regulating body weight inhibited differentiation of fat cells by 46 % compared to a control sample (p ⁇ 0.01).
  • Table 2 Composition of daily and night dosages for performing mutagenicity and citotoxicity assays.
  • the mutagenic activity of the extract mixture in the daily and night capsules was determined by the Salmonella microsome reverse mutation assay (Ames assay) with a standard assay of plate incorporation (Maron in Ames, 1983). The test was performed on two strains of bacteria Salmonella typhiamrium TA98 and TA100 (Trinova Biochem GmbH, Germany) in the presence and absence of the metabolic activation S9 (4 %) following OECD guidelines 471. The concentrations tested with the Ames assay were 0, 0.03125, 0.0625, 0.125, 0.25 and 0.5 mg/plate. 50 % ethanol was used as a solvent.
  • HepG2 cells The cytotoxic and genotoxic activity of the extract mixture in the daily and night capsules was determined by the MTT cytotoxicity assay (Mosmann, 1983) and with the microcore assay (Fenech, 2006) (following the OECD guidelines 487) on a human hepatoma cell line (HepG2 cells; source: Dr. Firouz Darroudi, Department of Toxicogenetics, the University of Leiden, the Netherlands). HepG2 cells have preserved some metabolic enzymes of Phase I and II of xenobiotic metabolism and are therefore a useful model for detecting the genotoxic activity of pro-genotoxins.
  • MTT assay was used to determine the cytotoxic activity of the extract mixture in the daily and night capsules (0, 0.03125, 0.0625, 0.125, 0.25 and 0.5 g/ml) after 24-hour exposure of cells.
  • the extract mixture in the form of both the daily and night capsules was tested in an extensive clinical trial carried out in compliance with Good Clinical Practice (Effect of a dietary supplement on body mass reduction in overweight persons with a simultaneous reduction in daily intake of supplements and exercise at least three times a week).
  • the clinical trial was randomized, double blind, placebo controlled, multi-centre. The trial took 14 weeks. It included 167 participants, women and men, aged from 18 to 65 and having overweight (body mass index (BMI): 25 kg/m2 - 30 kg/m2).
  • BMI body mass index
  • the extract mixture has proved to be safe as it even had less side effects compared to the placebo.
  • the results of the clinical trial are as follows:
  • the extract mixture helped both women and men in reducing their body mass, reducing waist size and improving some cardiovasular parameters, and also in improving their mood, sleep and reducing appetite and hunger.
  • the extract mixture also had positive cardiovascular effects. Compared to the control group, as much as 14 % of the women who were given the extract mixture, experienced reduction in the bottom blood pressure by an average of 2.1 mm Hg, while the bottom blood pressure in the control group was reduced by an average of merely 0.6 mm Hg.
  • the extract mixture helped the men, especially those having a lower BMI (25-27.5 kg/m2), reduce the body mass. 20 % more men from the mentioned group lost an average of 2.1 kg, while the men in the control group lost an average of merely 1.7 kg. Compared to the control group, 14 % more men who were given the extract mixture reduced their waist size. The men who were given the extract mixture have lost an average of 3.9 cm, while those in the control group have only lost 2.4 cm. Like the women, the men who were given the extract mixture also experienced the improvement in their bottom blood pressure, a three-fold improvement.
  • the trial has shown that the extract mixture significantly contributed to the reduction in body mass especially in those persons weighing from 86 to 95 kg.

Landscapes

  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

L'invention concerne un mélange utilisé comme complément alimentaire pour réduire la masse corporelle. Le mélange comprend six extraits de plantes: de l'extrait de feuilles de romarin (Rosmarinus officinalis), de l'extrait de café vert (Coffea Canephora robusta P.), de l'extrait de racines de radis noir (Raphanus sativus), de l'extrait de feuilles de thé vert (Camelia sinensis), de l'extrait d'écorce de cannelle (Cinnamonum cassia Presl) et de l'extrait de racines de roses (Rhodiola rosea L.). Le mélange contribue à réduire la masse corporelle et le tour de taille, à améliorer certains paramètres cardiovasculaires (la tension artérielle, le rythme cardiaque, le taux de lipides et de glucose dans le sang) ainsi qu'à améliorer certains aspects personnels (humeur, sommeil, faim, appétit).
PCT/SI2014/000067 2013-12-23 2014-11-13 Mélange permettant de réduire la masse corporelle WO2015099616A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
SI201300442A SI24563A (sl) 2013-12-23 2013-12-23 Mešanica za zmanjšanje telesne mase
SIP-201300442 2013-12-23
SIP-201400393 2014-10-24
SI201400393 2014-10-24

Publications (1)

Publication Number Publication Date
WO2015099616A1 true WO2015099616A1 (fr) 2015-07-02

Family

ID=52345492

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SI2014/000067 WO2015099616A1 (fr) 2013-12-23 2014-11-13 Mélange permettant de réduire la masse corporelle

Country Status (1)

Country Link
WO (1) WO2015099616A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019116241A1 (fr) * 2017-12-15 2019-06-20 Stefano Sala Complément alimentaire
ES2739133A1 (es) * 2018-07-20 2020-01-29 Fund Imdea Alimentacion Composicion para la reduccion del declive metabolico asociado al envejecimiento y/o el tratamiento de trastornos relacionados al metabolismo lipidico
WO2022180076A1 (fr) * 2021-02-25 2022-09-01 Givaudan Sa Compositions

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050249827A1 (en) * 2004-04-30 2005-11-10 Gardiner Paul T Nutritional composition which promotes weight loss, burns calories, increases thermogenesis, supports energy metabolism and/or suppresses appetite
US20060159773A1 (en) * 2005-01-20 2006-07-20 Stephen Holt Herbal compositions containing hoodia
KR20070009208A (ko) * 2005-07-15 2007-01-18 부산대학교 산학협력단 항비만 기능성 백김치 및 그 제조방법
WO2011047321A1 (fr) * 2009-10-16 2011-04-21 Naturex S.A. Extrait de feuilles de romarin pour applications pharmaceutiques

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050249827A1 (en) * 2004-04-30 2005-11-10 Gardiner Paul T Nutritional composition which promotes weight loss, burns calories, increases thermogenesis, supports energy metabolism and/or suppresses appetite
US20060159773A1 (en) * 2005-01-20 2006-07-20 Stephen Holt Herbal compositions containing hoodia
KR20070009208A (ko) * 2005-07-15 2007-01-18 부산대학교 산학협력단 항비만 기능성 백김치 및 그 제조방법
WO2011047321A1 (fr) * 2009-10-16 2011-04-21 Naturex S.A. Extrait de feuilles de romarin pour applications pharmaceutiques

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ZHENG G ET AL: "ANTI-OBESITY EFFECTS OF THREE MAJOR COMPONENTS OF GREEN TEA, CATECHINS, CAFFEINE AND THEANING, IN MICE", IN VIVO: INTERNATIONAL JOURNAL OF EXPERIMENTAL AND CLINICAL PATHOPHYSIOLOGY AND DRUG RESEARCH, INTERNATIONAL INSTITUTE OF ANTICANCER RESEARCH, GR, vol. 18, no. 1, 1 January 2004 (2004-01-01), pages 55 - 62, XP009060486, ISSN: 0258-851X *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019116241A1 (fr) * 2017-12-15 2019-06-20 Stefano Sala Complément alimentaire
ES2739133A1 (es) * 2018-07-20 2020-01-29 Fund Imdea Alimentacion Composicion para la reduccion del declive metabolico asociado al envejecimiento y/o el tratamiento de trastornos relacionados al metabolismo lipidico
WO2022180076A1 (fr) * 2021-02-25 2022-09-01 Givaudan Sa Compositions

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