WO2015085516A1

WO2015085516A1 - A nutritional reinforced composition and a process for preparation thereof

Info

Publication number: WO2015085516A1
Application number: PCT/CN2013/089100
Authority: WO
Inventors: Wenbao Zhang
Original assignee: Dsm Ip Assets B.V.
Priority date: 2013-12-11
Filing date: 2013-12-11
Publication date: 2015-06-18
Also published as: CN105813480A

Abstract

The invention provides an aqueous solution of a fat-soluble vitamin, which is clear and transparent and does not produce turbidity or precipitation after long-time settlement and oil circle after adding the composition into beverage. Accordingly, the nutritional reinforced composition is suitable for being as beverage or be as vitamin reinforcement additive of beverage. The invention further provides a process for preparing the aqueous solution.

Description

A NUTRITIONAL REINFORCED COMPOSITION AND A PROCESS FOR PREPARATION

THEREOF

Technical Filed

The invention is related to a nutritional reinforced composition and a process for preparation thereof. In particular, the invention is related to a clear aqueous solution of fat-soluble vitamin and a process for preparation thereof.

Background of the Invention

Vitamin is one of essential nutrients to keep human healthy, comprising fat-soluble vitamin and water-soluble vitamin. The fat-soluble vitamin includes vitamin A, vitamin D, vitamin E and Vitamin K.

The fat-soluble vitamin is not dissolvable in the water and thereby can hardly form a solution in water. This leads to difficulty in reinforcing nutritionally the said vitamin. For instance, generally, after adding the fat-soluble vitamin to the aqueous beverage, it is difficult to form a clear and transparent solution so as to affect the apparent of the product.

In order to overcome the foregoing difficulties, the common practice at present is to dissolve the fat-soluble vitamin into a lipid solvent such as vegetable oils, and then add into water to form an emulsion before adding the emulsion into an aqueous beverage. However, after adding the said emulsion into the beverage, turbidity is produced with some oil circles. Therefore, a new clear and transparent aqueous solution of fat-soluble vitamin suitable for nutritional reinforcement is in need.

Description of the Invention

The invention provides a new clear and transparent aqueous solution of fat-soluble vitamins suitable for nutritional reinforcement. In particular, the invention provides a nutritional reinforced composition, comprising a fat-soluble vitamin, Tween-80, glycerin and water, wherein the weight ratio of the fat-soluble vitamin, Tween-80 and glycerin is 1 : 2-15 : 2-10, and the water is sufficient to make the composition to form a solution. The nutritional reinforced composition is a clear and transparent aqueous solution, which thereby is suitable for being added into various beverages to reinforce vitamins.

Preferably, according to the nutritional reinforced composition of the present invention, the weight ratio of the fat-soluble vitamin, Tween-80 and glycerin is 1 : 5-15 : 2.5-8, more preferably 1 : 7-10 : 2.5-5, most preferably 1 : 7.5 : 2.5.

In an embodiment of the present invention, the nutritional reinforced composition comprises 2%-10% by weight of the fat-soluble vitamin, 10%-30% by weight of Tween-80 and 10%-20% by weight of glycerin. In a preferred embodiment of the present invention, the nutritional reinforced composition comprises 2%-6% by weight of the fat-soluble vitamin, 15%-25% by weight of Tween-80 and 10%-15% by weight of glycerin. In a more preferred embodiment of the present invention, the nutritional reinforced composition comprises 2%-6% by weight of the fat-soluble vitamin, 15%-20% by weight of Tween-80 and 10%-15% by weight of glycerin. In the most preferred embodiment of the present invention, the nutritional reinforced composition comprises 2%, 4%, 5.6% or 6% by weight of the fat-soluble vitamin, 15%, 20% or 25% by weight of Tween-80 and 15% by weight of glycerin.

The fat-soluble vitamin is known in the art and includes vitamin A, vitamin D, vitamin E and vitamin . Vitamin A may be acetate vitamin A, palmitic acid vitamin A and/or trans-retinoids. Vitamin D may be ergocalciferol (vitamin D₂) and/or cholecalciferol (vitamin D3}. Vitamin E may be d-oc-tocopherol, dl-oc-tocopherol, d-a-tocopheryl acetate, dl-a-tocopheryl acetate, mixed tocopherol concentrated liquid, d-a-tocopheryl succinate and/or dl-a-tocopheryl succinate. Vitamin may be vitamin Ki and/or vitamin K₂. Optionally, the nutritional reinforced composition of the invention further comprises additional additives, such as an antioxidant and/or an antiseptic. The examples of the antioxidant include but are not limited to dibutylhydroxytoluene, tea polyphenols, phytic acid, sodium sulfite, sodium bisulfite, sodium metabisulfite and sodium thiosulfate, preferably dibutylhydroxytoluene. The examples of the antiseptic include but are not limited into methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, butyl paraoxybenzoate, benzoic acid, sodium benzoate and sorbic acid, preferably sodium benzoate.

Optionally, the nutritional reinforced composition of the invention further comprises a pH adjuster. The examples of the pH adjuster include but are not limited into sodium hydroxide, potassium chloride, sodium chloride, phosphate such as disodium hydrogen phosphate, carbonate, citric acid, citrate, nicotinamide and benzamide, preferably citric acid and/or disodium hydrogen phosphate. It is understandable for one ordinary skilled in the art that some of the pH adjuster may also be used as an antioxidation synergist, for example, disodium hydrogen phosphate.

The invention also provides a process for preparing the nutritional reinforced composition of the invention, comprising:

1) adding a fat-soluble vitamin optionally mixed with an antioxidant into Tween-80 and mixing well;

2) adding glycerin into the obtained mixture and mixing well; and

3) adding water optionally mixed with an antiseptic and/or a pH adjuster into the obtained mixture, mixing well and then settling,

wherein the weight ratio of the added fat-soluble vitamin, Tween-80 and glycerin is 1 : 2-15 : 2-10, and water is sufficient to form a solution. Preferably, the weight ratio of the added fat-soluble vitamin, Tween-80 and glycerin is 1 : 5-15 : 2.5-8, more preferably 1 : 7-10 : 2.5-5, most preferably 1 : 7.5 : 2.5.

According to the process of the invention, the examples of the antioxidants, antiseptics and pH adjusters are mentioned as above. Preferably, the said antioxidant may be

2

RECT1FED SHEET ( RULE 91 ) dibutylhydroxytoluene, the said antiseptic may be sodium benzoate and the said pH adjuster may be citric acid and/or disodium hydrogen phosphate.

The advantage of the nutritional reinforced composition of the invention is that it is a clear and transparent aqueous solution, turbidity or precipitation is not produced after long-time settlement and no oil circle is emerged after adding the composition into beverage. Accordingly, the nutritional reinforced composition is suitable for being as beverage or be as vitamin reinforcement additive of beverage. Description of the Drawings

Figure 1 shows the clarification results of the compositions 1-3 observed by eye. From left to right, it is standard sample, composition 1, composition 2 and composition 3;

Figure 2 shows the clarification results of the compositions 4-6 observed by eye. From left to right, it is composition 4, composition 5 and composition 6; and

Figure 3 shows the clarification results of the compositions 7-9 observed by eye. From the left to right, it is composition 7, composition 8 and composition 9.

Examples

Example 1

The nutritional reinforced compositions 1-3 comprising vitamin E were prepared in accordance with the below steps and the amount listed in table 1.

1. Preparation of aqueous solution

Sodium benzoate, disodium hydrogen phosphate and citric acid were added into water at 50-60°C. After dissolution, the solution was kept at 50°C to obtain an aqueous solution.

2. Preparation of oily solution

Dibutylhydroxytoluene was added into vitamin E. After stirring well, they were added into Tween-80. Stirring was started for 10 mins at 45 °C and then stop to add glycerin. Stirring was restarted for 5 mins at 45 °C to obtain an oily solution.

3. The aqueous solution was mixed with the oily solution and then was stirred for 2 mins before settled for 20 mins. Then the obtained solution was bottling.

Table 1

Component Compositionl Composition2 Composition3 dl-a-tocopheryl acetate 2 g 4 g 6 g

Tween 80 15 g 20 g 25 g

glycerin 15 g 15 g 15 g

sodium benzoate 0.25 g 0.25 g 0.25 g

disodium hydrogen

0.2 g 0.2 g 0.2 g

phosphate

dibutylhydroxytoluene 0.1 g 0.1 g 0.1 g citric acid 0.025 g 0.025 g 0.025 g water 67.425 g 60.425 g 53.425 g

Example 2

The nutritional reinforced compositions 4-6 comprising vitamin A and D were prepared in accordance with the below steps and the amount listed in table 2.

1. Preparation of aqueous solution

2. Preparation of oily solution

Dibutylhydroxytoluene was added into vitamin A and D₃. After stirring well, they were added into Tween-80. Stirring was started for 10 mins at 45 °C and then stop to add glycerin. Stirring was restarted for 5 mins at 45 °C to obtain an oily solution.

Table 2

Example 3

The nutritional reinforced compositions 7-9 comprising vitamin A, vitamin D and vitamin E were prepared in accordance with the below steps and the amount listed in table 3.

1. Preparation of aqueous solution

2. Preparation of oil-soluble solution Dibutylhydroxytoluene was added into vitamin A, vitamin D3 and vitamin E. After stirring well, they were added into Tween-80. Stirring was started for 10 mins at 45 °C and then stop to add glycerin. Stirring was restarted for 5 mins at 45 °C to obtain an oily solution. 3. The aqueous solution was mixed with the oily solution and then was stirred for 2 mins before settled for 20 mins. Then the obtained solution was bottling.

Table 3

Example 4

The above compositions 1-9 were prepared in accordance with the process of examples 1-3. At the room temperature, the turbidity values of these compositions were determined with 2100N turbidity meter (Hach (China] Ltd., Shanghai) according to the instructions of the manufacturer, and then the clarification of these compositions were observed by eye with using graph paper as background and compared with the standard sample with turbidity value of 20 NTU (Hach (China) Ltd., Shanghai).

The obtained turbidity values of these compositions were shown in table 4. Table 4

Composition No.: Turbidity value (NTU)

1 6.48

2 6.75

3 13.7

4 8.52

5 32.9

6 10.1

7 7.85 As shown in table 4, the turbidity values of the compositions are less than 20 NTU except for the composition 5. Figures 1-3 show the clarification results of the compositions 1-9 and the standard sample with turbidity value of 20 NTU observed by eye. It was obvious that these compositions are clear and transparent solutions.

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RECTIFED SHEET ( RULE 91 )

Claims

1. A nutritional reinforced composition, comprising a fat-soluble vitamin, Tween-80, glycerin and water, wherein the weight ratio of the fat-soluble vitamin, Tween-80 and glycerin is 1:2-15:2-10 and the water is sufficient to make the composition to form a solution.

2. The nutritional reinforced composition according to Claim 1, wherein the weight ratio of the fat-soluble vitamin, Tween-80 and glycerin is 1 : 5-15 : 2.5-8.

3. The nutritional reinforced composition according to Claim 2, wherein the weight ratio of the fat-soluble vitamin, Tween-80 and glycerin is 1 : 7-10 : 2.5-5.

4. The nutritional reinforced composition according to Claim 3, wherein the weight ratio of the fat-soluble vitamin, Tween-80 and glycerin is 1 : 7.5 : 2.5.

5. The nutritional reinforced composition according to Claim 1, comprising 2%-10% by weight of the fat-soluble vitamin, 10%-30% by weight of Tween-80 and 10%-20% by weight of glycerin.

6. The nutritional reinforced composition according to Claim 5, comprising 2%-6% by weight of the fat-soluble vitamin, 15%-25% by weight of Tween-80 and 10%-15% by weight of glycerin.

7. The nutritional reinforced composition according to Claim 5, comprising 2%-6% by weight of the fat-soluble vitamin, 15%-20% by weight of Tween-80 and 10%-15% by weight of glycerin.

8. The nutritional reinforced composition according to Claim 5, comprising 2%, 4%, 5.6% or 6% by weight of the fat-soluble vitamin, 15%, 20% or 25% by weight of Tween-80 and

15% by weight of glycerin.

9. The nutritional reinforced composition according to Claim 1, wherein the fat-soluble vitamin is vitamin A, D, E and/or K.

10. The nutritional reinforced composition according to Claim 9, wherein the vitamin A is acetate vitamin A, palmitic acid vitamin A and/or trans-retinoids; vitamin D is ergocalciferol and/or cholecalciferol; Vitamin E is d-cc-tocopherol, dl-cc-tocopherol, d-ot-tocopheryl acetate, dl-a-tocopheryl acetate, mixed tocopherol concentrated liquid, d-cc-tocopheryl succinate and/or dl-a-tocopheryl succinate; and vitamin K is vitamin Ki and/or vitamin K₂.

11. The nutritional reinforced composition according to Claim 11, further comprising additional additive.

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RECTTCED SHEET (RULE 91)

12. The nutritional reinforced composition according to Claim 11, wherein the additional additive is an antioxidant, an antiseptic and/or a pH adjuster.

13. The nutritional reinforced composition according to Claim 12, wherein the antioxidant is selected from the group consisting of dibutylhydroxytoluene, tea polyphenols, phytic acid, sodium sulfite, sodium bisulfite, sodium metabisulfite and sodium thiosulfate, preferably dibutylhydroxytoluene; the antiseptic is selected from the group consisting ofmethyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, butyl paraoxybenzoate, benzoic acid, sodium benzoate and sorbic acid, preferably sodium benzoate; and pH adjuster is selected from the group consisting of sodium hydroxide, potassium chloride, sodium chloride, phosphate such as disodium hydrogen phosphate, carbonate, citric acid, citrate, nicotinamide and benzamide, preferably citric acid and/or disodium hydrogen phosphate.

14. A process of preparing the nutritional reinforced composition according to any one of Claims 1-13, comprising:

2) adding glycerin into the obtained mixture and mixing well; and

wherein the weight ratio of the added fat-soluble vitamin, Tween-80 and glycerin is 1 : 2-15 : 2-10, and water is sufficient to form a solution.