WO2015080192A1 - Puce de contrôle - Google Patents

Puce de contrôle Download PDF

Info

Publication number
WO2015080192A1
WO2015080192A1 PCT/JP2014/081337 JP2014081337W WO2015080192A1 WO 2015080192 A1 WO2015080192 A1 WO 2015080192A1 JP 2014081337 W JP2014081337 W JP 2014081337W WO 2015080192 A1 WO2015080192 A1 WO 2015080192A1
Authority
WO
WIPO (PCT)
Prior art keywords
reagent
unit
angle
wall surface
sample
Prior art date
Application number
PCT/JP2014/081337
Other languages
English (en)
Japanese (ja)
Inventor
由美子 大鹿
千里 吉村
Original Assignee
ブラザー工業株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ブラザー工業株式会社 filed Critical ブラザー工業株式会社
Publication of WO2015080192A1 publication Critical patent/WO2015080192A1/fr

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0621—Control of the sequence of chambers filled or emptied
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00495—Centrifuges
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/07—Centrifugal type cuvettes

Definitions

  • the present disclosure relates to a test chip having a mixing unit in which a specimen and a reagent are mixed.
  • an inspection chip for inspecting a specimen such as a biological substance or a chemical substance
  • the inspection chip disclosed in Patent Document 1 includes a V-shaped quantification unit as viewed from the front.
  • a specimen or a reagent is injected into the quantification unit by applying a centrifugal force to the entrance direction of the V-shaped quantification unit.
  • the sample or reagent is quantified in the quantification unit without changing the direction of the centrifugal force.
  • the specimen or reagent quantified by changing the direction of the centrifugal force is used for the examination.
  • the sample or reagent overflows from the quantification unit when the sample or reagent is quantified in the quantification unit.
  • the volume of the sample or reagent up to the quantification surface which is the liquid level at the time of overflow, is quantified.
  • the purpose of the present disclosure is to provide a test chip that improves quantitative accuracy.
  • the first connection part that is a connection point with the first guide part that communicates with the mixing part, and in the quantification part, the communication with the surplus part.
  • a second connection portion that is a connection point with the second guide portion, wherein the first guide portion is composed of a first wall surface and a second wall surface at different angles, and the first wall surface is the fixed amount at the first connection portion.
  • Connected to the second part A surface is connected to the first wall surface and the mixing unit, and is parallel to a fixed surface connecting the first connection unit and the second connection unit, and an extension of the wall surface on the first guide unit side of the supply unit.
  • the third angle formed by the extending direction of the wall surface of the second guide portion to be connected, the fourth angle formed by the direction parallel to the fixed surface and the extending direction of the first wall surface are: 0 ⁇ first angle ⁇ third angle ⁇ second angle and The relationship is 0 ⁇ fourth angle ⁇ third angle.
  • the second angle is larger than the first angle when the specimen or the reagent is injected into the quantification unit through the first guide unit along the external force such as centrifugal force or gravity from the supply unit. Therefore, it is possible to reduce the possibility that the specimen or reagent flows out from the first guide unit to the mixing unit side. Further, since the second angle is larger than the third angle, the sample or reagent does not flow out from the first guide unit to the mixing unit side, and the surplus sample or reagent can flow to the surplus unit side. In addition, since the second angle is larger than the fourth angle, the specimen or reagent is placed between the second wall surface of the first guide part that forms the second angle and the first wall surface of the first guide part that forms the fourth angle. It becomes possible to hold.
  • the sample or reagent quantification sample or reagent in the quantification unit is insufficient. The possibility of doing so can be reduced. As a result, at the time of injection into the quantification unit, the possibility of the sample or reagent flowing out from the first guide unit to the mixing unit side is reduced, and the possibility of a decrease in quantification accuracy due to a shortage of the sample or reagent is reduced. In addition, a decrease in inspection accuracy can be reduced.
  • the third angle is larger than the first angle
  • the sample or reagent overflowing from the quantification unit follows the wall surface of the second guide unit. Therefore, it becomes easy to flow into the surplus part side.
  • the third angle is larger than the fourth angle
  • the sample or reagent overflowing from the quantification unit does not flow to the first guide unit side when the sample or reagent is injected from the supply unit to the quantification unit. Moreover, it becomes easy to flow into the surplus part side along the wall surface of a 2nd guide part.
  • a said 2nd wall surface is provided with the 1st end part which is an edge part on the opposite side to a connection part with a said 1st wall surface, and connects to the said 1st connection part and a direction parallel to the said fixed surface.
  • the fifth angle formed by the wall surface of the quantitative unit may be larger than the sixth angle formed by a direction parallel to the quantitative surface and a straight line connecting the first connection unit and the first end.
  • the test chip includes a third guide unit that guides the sample or the reagent from the supply unit toward the quantification unit, and a second end that is an end of the third guide unit close to the quantification unit includes:
  • the second connecting portion and the first end portion may be located on the opposite side of the quantitative portion from the straight line connecting the second connecting portion and the first end portion.
  • the test chip extends in the extending direction from the holding unit toward the supply unit, and holds the sample or reagent supplied to the supply unit, and communicates the holding unit and the supply unit.
  • An inclined surface that passes through a third end portion that is an end portion of the third guide portion opposite to the second end portion, and is closer to the extending direction side than the inclined surface.
  • a seventh angle formed in a direction parallel to the quantitative surface may be smaller than the sixth angle.
  • FIG. 6 is a state transition diagram showing the state of the first reagent 18 in the supply unit 132 of the test chip 2.
  • FIG. 8 is a flowchart of a centrifugation process. It is a state transition diagram of the test
  • FIG. 8 is a state transition diagram of the test chip 2 continued from FIG. 7.
  • FIG. 9 is a state transition diagram of the test chip 2 continued from FIG. 8.
  • FIG. 4 is a partial enlarged view of the front surface of the test chip 2 showing a state in which the first reagent 18 is held by the first guide portion 138.
  • FIG. FIG. 4 is a partially enlarged view of the front surface of the test chip 2 showing a state in which the first reagent 18 flows from the first guide part 138 to the reagent quantitative part 134A.
  • FIG. 1 shows a plane of the inspection apparatus 1 constituting the inspection system 3 and functional blocks inside the control apparatus 90.
  • the inspection system 3 of the present embodiment includes an inspection chip 2 that can store a sample and a reagent that are liquids, and an inspection apparatus 1 that performs an inspection using the inspection chip 2.
  • the inspection device 1 rotates the inspection chip 2 around the vertical axis A ⁇ b> 1 separated from the inspection chip 2, centrifugal force acts on the inspection chip 2.
  • the inspection device 1 rotates the inspection chip 2 about the horizontal axis A ⁇ b> 2, the centrifugal direction that is the direction of the centrifugal force acting on the inspection chip 2 is switched with respect to the inspection chip 2. Since the inspection system 3 and the inspection apparatus 1 of the present embodiment have a well-known structure as described in JP 2012-78107 A, the outline of the structure of the inspection apparatus 1 will be described in the following description.
  • the upper side, the lower side, the right side, the left side, the front side of the paper surface, and the back side of the paper surface in FIG. 1 are defined as the front side, the rear side, the right side, the left side, the upper side, and the lower side, respectively.
  • the direction of the vertical axis A1 is the vertical direction of the inspection apparatus 1
  • the direction of the horizontal axis A2 is the direction of the speed when the inspection chip 2 is rotated about the vertical axis A1.
  • FIG. 1 shows a state in which the top plate of the upper housing 30 of the inspection apparatus 1 has been removed.
  • the inspection apparatus 1 includes an upper housing 30, a lower housing 31, an upper plate 32, a turntable 33, an angle changing mechanism 34, and a control device 90.
  • the turntable 33 is a disk rotatably provided on the upper side of an upper plate 32 described later.
  • the inspection chip 2 is held above the turntable 33.
  • the angle changing mechanism 34 is a drive mechanism provided on the turntable 33.
  • the angle changing mechanism 34 rotates the inspection chip 2 around the horizontal axis A2.
  • the upper housing 30 is fixed to an upper plate 32 described later, and a measurement unit 7 that performs optical measurement on the inspection chip 2 is provided inside.
  • the control device 90 is a controller that controls various processes of the inspection device 1.
  • the schematic structure of the lower housing 31 will be described.
  • the lower housing 31 has a box-shaped frame structure in which frame members are combined.
  • An upper plate 32 that is a rectangular plate material is provided on the upper surface of the lower housing 31.
  • a drive mechanism that rotates the turntable 33 around the vertical axis A1 is provided in the lower housing 31 as follows.
  • a spindle motor 35 that supplies a driving force for rotating the turntable 33 is installed on the left side of the lower housing 31.
  • a shaft 36 of the main shaft motor 35 protrudes upward, and a pulley 37 is fixed.
  • a vertical main shaft 57 extending upward from the inside of the lower housing 31 is provided at the center of the lower housing 31.
  • the main shaft 57 passes through the upper plate 32 and protrudes above the lower housing 31.
  • the upper end portion of the main shaft 57 is connected to the center portion of the turntable 33.
  • the main shaft 57 is rotatably held by a support member (not shown) provided immediately below the upper plate 32.
  • a pulley 38 is fixed to the main shaft 57 below the support member.
  • a belt 39 is stretched over the pulley 37 and the pulley 38.
  • a guide rail (not shown) extending in the vertical direction inside the lower housing 31 is provided on the right side in the lower housing 31.
  • a T-shaped plate (not shown) is movable in the vertical direction in the lower housing 31 along the guide rail.
  • the above-described main shaft 57 is a hollow cylindrical body.
  • An inner shaft (not shown) is a shaft that can move in the vertical direction inside the main shaft 57.
  • the upper end portion of the inner shaft passes through the main shaft 57 and is connected to the rack gear 43.
  • a bearing (not shown) is provided at the left end of the T-shaped plate. Inside the bearing, the lower end portion of the inner shaft is rotatably held.
  • a stepping motor 51 for moving the T-shaped plate up and down is fixed in front of the T-shaped plate.
  • the shaft 58 of the stepping motor 51 protrudes rearward, that is, downward in FIG.
  • a disc-shaped cam plate (not shown) is fixed to the tip of the shaft 58.
  • a cylindrical projection (not shown) is provided on the rear surface of the cam plate.
  • the tip of the protrusion is inserted into a groove (not shown). The protrusion can slide in the groove.
  • the angle changing mechanism 34 has a pair of L-shaped plates 60 fixed to the upper surface of the turntable 33. Each L-shaped plate 60 extends upward from a base portion fixed in the vicinity of the center of the turntable 33, and its upper end portion extends outward in the radial direction of the turntable 33.
  • a rack gear 43 (not shown) fixed to the inner shaft is provided between the pair of L-shaped plates 60.
  • the rack gear 43 is a metal plate-like member that is long in the vertical direction, and gears are respectively carved on both end faces.
  • a horizontal support shaft 46 having a gear 45 is rotatably supported at the distal end side in the extending direction of each L-shaped plate 60.
  • the support shaft 46 is fixed to the inspection chip 2 via a mounting holder (not shown). For this reason, the inspection chip 2 also rotates around the support shaft 46 in conjunction with the rotation of the gear 45.
  • a pinion gear 44 supported by an L-shaped plate 60 so as to be rotatable about a horizontal axis (not shown) is interposed.
  • the pinion gear 44 meshes with the gear 45 and the rack gear 43, respectively. In conjunction with the vertical movement of the rack gear 43, the pinion gear 44 and the gear 45 are driven to rotate, and the inspection chip 2 is rotated about the support shaft 46.
  • the inspection chip 2 rotates about the main shaft 57, which is a vertical axis, and a centrifugal force acts on the inspection chip 2.
  • the rotation around the vertical axis A1 of the inspection chip 2 is referred to as revolution.
  • the inspection chip 2 rotates about the support shaft 46 which is a horizontal axis, and the direction of the centrifugal force acting on the inspection chip 2 changes relatively.
  • the rotation around the horizontal axis A2 of the inspection chip 2 is called autorotation.
  • the rack gear 43 is also lowered to the lowermost end of the movable range.
  • the inspection chip 2 is in a steady state where the rotation angle is 0 degree.
  • the rack gear 43 is also raised to the uppermost end of the movable range.
  • inspection chip 2 will be in the state rotated 180 degree
  • the upper housing 30 has a box-like frame structure in which frame members are combined, and is installed on the upper left side of the upper plate 32. More specifically, the upper housing 30 is provided outside the range in which the inspection chip 2 is rotated as viewed from the main shaft 57 at the rotation center of the turntable 33.
  • the measurement unit 7 provided in the upper housing 30 includes a light source 71 that emits measurement light, and an optical sensor 72 that detects the measurement light emitted from the light source 71.
  • the light source 71 and the optical sensor 72 are disposed on both the front and rear sides of the turntable 33 outside the rotation range of the inspection chip 2.
  • the position on the left side of the main shaft 57 in the reciprocable range of the inspection chip 2 is the measurement position at which the inspection chip 2 is irradiated with the measurement light.
  • the measurement light connecting the light source 71 and the optical sensor 72 intersects the front surface and the rear surface of the inspection chip 2 substantially perpendicularly.
  • the control device 90 includes a CPU 91 that performs main control of the inspection device 1, a RAM 92 that temporarily stores various data, and a ROM 93 that stores a control program. Connected to the CPU 91 are an operation unit 94 for a user to input instructions to the control device 90, a hard disk device 95 for storing various data and programs, and a display 96 for displaying various information.
  • a personal computer may be used, or a dedicated control device may be used.
  • the revolution controller 97, the rotation controller 98, and the measurement controller 99 are connected to the CPU 91.
  • the revolution controller 97 controls the revolution of the inspection chip 2 by transmitting a control signal for rotating the spindle motor 35 to the spindle motor 35.
  • the rotation controller 98 controls the rotation of the inspection chip 2 by transmitting a control signal for rotating the stepping motor 51 to the stepping motor 51.
  • the measurement controller 99 performs the optical measurement of the inspection chip 2 by driving the measurement unit 7. Specifically, the measurement controller 99 transmits a control signal for executing light emission of the light source 71 and light detection of the optical sensor 72 to the light source 71 and the optical sensor 72.
  • the CPU 91 controls the revolution controller 97, the rotation controller 98 and the measurement controller 99.
  • FIG. 2 Structure of inspection chip 2> With reference to FIG.2 and FIG.3, the detailed structure of the test
  • the upper, lower, left, right, front side, and back side of FIG. 2 are the upper, lower, left, right, front, and rear sides of the inspection chip 2, respectively. .
  • the inspection chip 2 has a square shape when viewed from the front as an example, and mainly includes a transparent synthetic resin plate 20 having a predetermined thickness.
  • the front surface 201 of the plate member 20 is sealed with a sheet 291 made of a transparent synthetic resin thin plate.
  • the rear surface 202 opposite to the front surface 201 is sealed with a sheet 292 made of a transparent synthetic resin thin plate.
  • a liquid flow path 25 is formed between the plate material 20 and the sheet 291 and between the plate material 20 and the sheet 292 so that the liquid sealed in the inspection chip 2 can flow. Has been.
  • the liquid channel 25 is a recess formed at a predetermined depth on the front surface 201 side and the rear surface 202 side of the plate material 20, and extends in a direction orthogonal to the front-rear direction, which is the thickness direction of the plate material 20.
  • the sheets 291 and 292 seal the flow path forming surface of the plate material 20. The sheets 291 and 292 are not shown except for FIGS.
  • the liquid channel 25 includes the sample quantitative channel 11, the reagent quantitative channels 13, 15, the first connection channel 301, the second connection channel 331, the mixing unit 80, the measurement unit 81, and the like.
  • the reagent fixed amount flow path 13 is provided in the upper left part of the front surface 201.
  • the sample quantitative flow path 11 is provided on the right side of the reagent quantitative flow path 13 in the front surface 201.
  • the reagent fixed amount flow path 15 is provided in the upper left part on the rear surface 202 side.
  • the mixing unit 80 is provided in the lower right part of the front surface 201.
  • the mixing unit 80 is an area that includes a channel on the right side of an end 315 (described later) and an inlet 306 (described later) that is connected to a channel 117 (described later) and extends downward.
  • the measurement unit 81 is a lower part of the mixing unit 80.
  • the reagent quantification channels 13 and 15 include the inlet 130, the reagent holding unit 131, the supply unit 132, the communication path 154, the reagent quantification unit 134, the first guide unit 138, and the first, respectively.
  • a second guide portion 137, a third guide portion 139, and a surplus portion 136 are included.
  • the reagent holding part 131 is provided in the upper left part of the test chip 2.
  • the reagent holding part 131 is a recess that opens upward.
  • the injection port 130 penetrates the plate member 20 from the upper part of the reagent holding part 131 toward the upper side part 21 of the test chip 2.
  • the inlet 130 is a part where the first reagent 18 or the second reagent 19 is injected into the reagent holding part 131.
  • the reagent holding part 131 of the reagent fixed amount flow channel 13 is a part where the first reagent 18 injected from the injection port 130 of the reagent fixed amount flow channel 13 is stored.
  • the reagent holding part 131 of the reagent fixed amount flow channel 15 is a part where the second reagent 19 injected from the injection port 130 of the reagent fixed amount flow channel 15 is stored.
  • the second reagent 19 of the present embodiment is a reagent that is mixed after the first reagent 18 and a specimen 17A described later are mixed. In the following description, the first reagent 18 and the second reagent 19 are collectively referred to as “reagent 16” when not specified either.
  • the supply unit 132 is a flow channel extending downward from the upper right part of the reagent holding unit 131.
  • the lower end part of the supply part 132 is connected with the 3rd guide part 139 which is a channel
  • a wall portion 27 is provided between the reagent holding unit 131 and the supply unit 132. The wall portion 27 extends obliquely upward to the right from the left side portion 23 toward the upper side portion 21.
  • a reagent quantitative unit 134 is provided below the third guide unit 139.
  • the third guide unit 139 guides the reagent 16 to the reagent quantitative unit 134.
  • the reagent quantification part 134 is a part where the reagent 16 is quantified, and is a concave part recessed in the lower left.
  • the communication path 154 extends in the extending direction from the supply unit 132 toward the reagent holding unit 131, and communicates the supply unit 132 and the reagent holding unit 131.
  • the reagent quantification unit 134 is connected to the mixing unit 80 through the first connection channel 301 and is connected to the surplus unit 136 through the second guide unit 137.
  • the end of the reagent quantification unit 134 on the mixing unit 80 side is referred to as a first connection unit 141.
  • the end of the reagent quantification unit 134 opposite to the mixing unit 80 is referred to as a second connection unit 142.
  • a surface connecting the first connection portion 141 and the second connection portion 142 is a reagent fixed amount surface 146.
  • the reagent quantification surface 146 is a virtual surface that is the position of the upper surface of the reagent 16 when the reagent 16 is quantified by the reagent quantification unit 134. Therefore, the volume of the liquid channel 25 below the reagent quantification surface 146 is the quantification amount in the reagent quantification unit 134.
  • the second guide part 137 extends obliquely downward to the left from the upper part of the reagent quantification part 134. That is, the second guide portion 137 extends from the second connection portion 142 toward the surplus portion 136.
  • the second guide unit 137 is a flow path through which the reagent 16 overflowing from the reagent quantitative unit 134 moves.
  • a reagent surplus part 136 is provided at the lower left of the reagent quantification part 134.
  • the reagent surplus part 136 is a part in which the reagent 16 that has moved through the second guide part 137 is accommodated, and is a concave part provided downward and rightward from the lower end part of the second guide part 137.
  • the first connection channel 301 will be described.
  • the reagent quantitative unit 134 of the reagent quantitative channel 13 is referred to as a reagent quantitative unit 134A
  • the reagent quantitative unit 134 of the reagent quantitative channel 15 is referred to as a reagent quantitative unit 134B.
  • the first connection channel 301 is a channel that is formed on the front surface 201 and connects the reagent quantitative unit 134A and the mixing unit 80.
  • the first connection channel 301 extends obliquely upward to the right from the upper part of the reagent quantitative unit 134A, extends downward from the right end part, and further extends to the right from the lower end part.
  • the first connection channel 301 is formed by a first wall surface 302 and a second wall surface 303.
  • the first wall surface 302 is a wall surface facing the reagent quantitative unit 134A and extending toward the mixing unit 80 side.
  • the first wall surface 302 extends from the lower end of the third guide portion 139 to a right end portion 314 that forms an inflow port 306 described later.
  • the second wall surface 303 is a wall portion facing the first wall surface 302.
  • the second wall surface 303 extends from the first connection portion 141 of the reagent quantitative unit 134A to a right end 313 that forms an inflow port 306 described later.
  • the first connection channel 301 includes a partial receiving part 304, a reagent receiving part 305, and an inflow port 306.
  • the partial receiving unit 304 is a part that holds a part of the first reagent 18 quantified by the reagent quantification unit 134A.
  • the partial receiver 304 is provided on the right side of the reagent quantitative unit 134A and on the reagent quantitative unit 134A side from a merging hole 351 described later.
  • the partial receiving portion 304 is a concave portion that opens in the left direction from the first connection portion 141 toward the second connection portion 142.
  • the partial receiving unit 304 has a capacity smaller than that of the reagent quantitative unit 134A.
  • the wall surface connected to the first connection portion 141 of the reagent quantification unit 134A is referred to as a reagent flow channel wall surface 308. That is, the reagent channel wall surface 308 is connected to the reagent quantitative unit 134A in the first connection channel 301 and forms a part of the first connection channel 301.
  • the reagent channel wall surface 308 is third guided by a virtual surface 320 which is a virtual surface obtained by extending the reagent metering surface 146 of the reagent metering channel 13 to the right side on the mixing unit 80 side in parallel with the reagent metering surface 146. It is inclined to the part 139 side.
  • the reagent channel wall surface 308 includes a first wall surface 308A and a second wall surface 308B.
  • the first wall surface 308 ⁇ / b> A extends obliquely upward to the right from the first connection portion 141, and is connected to the second wall surface 308 ⁇ / b> B at the connection portion 309.
  • the second wall surface 308B extends obliquely upward to the right from the connection portion 309.
  • An imaginary line 311 drawn from the first end 310 on the side of the mixing unit 80 in the reagent channel wall surface 308 in the direction perpendicular to the reagent channel wall surface 308 is the first connection flow on the reagent quantitative unit 134A side from the partial receiving unit 304.
  • a first wall surface 308A is formed at a lower end of the third guide portion 139 in a direction perpendicular to the wall surface 132B of the supply portion 132 constituting a first angle ⁇ 1 described later.
  • the reagent receiving unit 305 is provided below the partial receiving unit 304 between the partial receiving unit 304 and the mixing unit 80.
  • the reagent receiving part 305 is a concave part having an upper opening on the partial receiving part 304 side, and is a part for holding the first reagent 18 that moves downward after being held by the partial receiving part 304.
  • the reagent receiving part 305 is formed by the wall surface 303A, the wall surface 303B, and the wall surface 303C of the second wall surface 303.
  • the wall surface 303 ⁇ / b> A is a wall surface that extends vertically on the right side of the reagent surplus portion 136 of the reagent fixed amount flow path 13.
  • the wall surface 303B is a wall surface extending in the right direction from the lower end of the wall surface 303A.
  • the right end portion of the wall surface 303 ⁇ / b> B is located on the lower left side of the mixing unit 80.
  • the wall surface 303C is a wall surface extending obliquely upward to the right from the right end portion of the wall surface 303B.
  • the inflow port 306 is formed by the right end 313 of the wall surface 303C and the right end 314 of the first wall 302 located above the right end 313.
  • the inflow port 306 is located on the left side of the mixing unit 80 and is a part for allowing the reagent 16 to flow into the mixing unit 80.
  • a confluence hole portion 351 is provided at the center in the left-right direction at the lower end of the first connection channel 301.
  • the merge hole 351 is a hole that penetrates the plate member 20 in the front-rear direction and joins the second connection channel 331 to the first connection channel 301.
  • the end portion 352 is left and right along the wall surface 303 ⁇ / b> B of the second wall surface 303. Extend in the direction.
  • the end portion 353 is along the first wall surface 302.
  • the second connection channel 331 will be described. As shown in FIG. 3, the second connection channel 331 is a channel that is formed on the rear surface 202 and extends from the reagent quantitative unit 134 ⁇ / b> B toward the mixing unit 80 and connects the reagent quantitative unit 134 ⁇ / b> B and the mixing unit 80.
  • the second connection channel 331 includes four reagent receiving portions 341, 342, 343, and 344.
  • the reagent receiving units 341 to 344 receive the second reagent 19 quantified by the reagent quantifying unit 134B.
  • the reagent receiving part 341 is a concave part that is located on the upper right side of the reagent fixed quantity part 134B and opens to the left.
  • the reagent receiving part 342 is a recessed part that is located on the lower left side of the reagent receiving part 341 and opens upward.
  • the reagent receiving part 343 is a recessed part that is located on the lower right side of the reagent receiving part 342 and opens to the left.
  • the reagent receiving part 344 is a recessed part that is located below the reagent receiving part 343 and opens upward.
  • the second connection channel 331 extends obliquely upward and to the right from the reagent determination unit 134B and connects to the reagent receiver 341, and extends downward and obliquely to the left from the reagent receiver 341 and connects to the reagent receiver 342.
  • the second connection channel 331 extends obliquely upward to the right from the reagent receiving part 342, extends downward from the right end, and is connected to the reagent receiving part 343.
  • the second connection channel 331 extends obliquely downward to the left from the reagent receiving part 343 and is connected to the reagent receiving part 344.
  • the right end portion of the reagent receiving portion 344 is connected to the merge hole portion 351 and is connected to the first connection flow path 301 on the front surface 201 side.
  • the sample fixed amount flow path 11 includes an injection port 110, a sample holding unit 111, a sample supply unit 112, a sample guide unit 113, a separation unit 124, a channel 125, a channel 127, a sample surplus unit 126, A supply unit 123, a specimen quantification unit 114, a passage 115, a passage 117, and a second surplus unit 116 are included.
  • the sample holding unit 111 is provided on the right side of the supply unit 132 of the reagent fixed amount flow channel 13.
  • the sample holder 111 is a recess that opens upward.
  • the injection port 110 penetrates the plate member 20 from the upper part of the specimen holding part 111 toward the upper side part 21 of the test chip 2.
  • the injection port 110 is a part where the sample 17 is injected into the sample holding unit 111.
  • the sample holding unit 111 is a part where the sample 17 injected from the injection port 110 is stored.
  • the specimen 17 of the present embodiment is a liquid containing components such as blood, plasma, blood cells, bone marrow, urine, vaginal tissue, epithelial tissue, tumor, semen, saliva, or foodstuff.
  • the sample supply unit 112 is a flow path extending downward from the upper right part of the sample holding unit 111.
  • the lower end of the sample supply unit 112 is connected to a sample guide unit 113 which is a passage having a narrow channel.
  • a separation unit 124 is provided below the sample guide unit 113.
  • the sample guide unit 113 guides the sample 17 to the separation unit 124.
  • the separation unit 124 is a part where components contained in the specimen 17 are separated.
  • the separation part 124 is a recess that opens upward and tilts diagonally downward to the right.
  • the separation unit 124 centrifuges the specimen 17 into a component having a small specific gravity and a component having a large specific gravity by the action of centrifugal force.
  • a component having a small specific gravity of the sample 17 separated by the separation unit 124 is referred to as a sample 17A
  • a component having a large specific gravity is referred to as a sample 17B.
  • connection channel 120 extends obliquely upward to the right from the central portion in the vertical direction on the right side surface of the separation unit 124, and the upper end of the connection channel 120 is connected to the upper end of the component holding unit 121.
  • the component holding unit 121 is a storage unit that holds the sample 17A and a part of the sample 17B separated by the separation unit 124.
  • the width of the connecting channel 120 is narrower than the width of the channel 127 described later. Therefore, the specimen 17A flows out to the passage 127 before flowing into the connection channel 120. Therefore, the possibility that the sample 17A flows into the component holding unit 121 before the passage 127 can be reduced.
  • the passage 125 extends obliquely to the left and the passage 127 extends obliquely upward to the right.
  • the passage 125 extends to the specimen surplus portion 126 provided on the lower left side of the separation portion 124.
  • the sample surplus portion 126 is a portion where the sample 17 overflowing from the separation portion 124 is stored, and is a recess provided in the right direction and the downward direction from the lower end portion of the passage 125.
  • the passage 127 is connected to the second supply unit 123.
  • the second supply unit 123 is a flow path that extends downward from the upper right portion of the passage 127.
  • the lower end of the 2nd supply part 123 is connected with the 2nd guide part 128 which is a channel
  • Below the second guide unit 128, a sample quantitative unit 114 is provided below the second guide unit 128, a sample quantitative unit 114 is provided below the second guide unit 128, a sample quantitative unit 114 is provided below the second guide unit 128, a sample quantitative unit 114 is provided below the second guide unit 128, a sample quantitative unit 114 is provided below the second guide unit 128, a sample quantitative unit 114 is provided below the second guide unit 128, a sample quantitative unit 114 is provided below the second guide unit 128, a sample quantitative unit 114 is provided below the second guide unit 128, a sample quantitative unit 114 is provided below the second guide unit 128, a sample quantitative unit 114 is provided below the second guide unit 128, a sample
  • the specimen quantification unit 114 is connected to the mixing unit 80 via the passage 117 and is connected to the second surplus portion 116 via the passage 115.
  • the end of the sample determination unit 114 on the mixing unit 80 side is referred to as a first sample end 118.
  • the end of the sample determination unit 114 opposite to the mixing unit 80 is referred to as a second sample end 119. That is, the passage 115 extends from the second specimen end 119 to the second surplus portion 116.
  • a surface connecting the first sample end portion 118 and the second sample end portion 119 is a sample determination surface 129.
  • the sample quantification surface 129 is a virtual surface serving as the position of the upper surface of the sample 17A when the sample 17A is quantified by the sample quantification unit 114. Therefore, the volume of the liquid channel 25 below the sample quantification surface 129 is the quantification amount in the sample quantification unit 114.
  • the passage 115 extends obliquely to the left and the passage 117 extends obliquely upward to the right.
  • a second surplus part 116 is provided at the lower left of the sample quantification part 114.
  • the passage 115 is connected to the second surplus portion 116.
  • the second surplus part 116 is a part where the specimen 17A overflowing from the specimen quantification part 114 is stored.
  • the second surplus portion 116 is a recess provided in the right direction from the lower end portion of the passage 115.
  • the passage 117 is connected to the mixing unit 80.
  • a wall surface provided on the mixing unit 80 side of the sample quantitative unit 114 and connected to the sample quantitative unit 114 is referred to as a sample flow channel wall surface 312.
  • the sample flow channel wall surface 312 is inclined toward the second guide unit 128 side from a virtual surface 321 that extends the sample determination surface 129 to the right side that is the mixing unit 80 side in parallel with the sample determination surface 129. More specifically, the sample channel wall surface 312 extends obliquely upward to the right from the first sample end 118 of the sample determination unit 114.
  • a virtual plane parallel to the sample channel wall surface 312 in the mixing unit 80 is referred to as a virtual plane 317.
  • the position of the virtual surface 317 is such that the volume of the region 318 surrounded by the virtual surface 317 and the lower portion of the mixing unit 80, which is farther from the sample flow channel wall surface 312 than the virtual surface 317, is that of the reagent quantification unit 134A.
  • the position is equal to the differential capacity obtained by subtracting the capacity of the partial receiving unit 304 from the capacity.
  • a virtual line 316 drawn from the end portion 315 of the sample channel wall surface 312 on the mixing unit 80 side in a direction perpendicular to the sample channel wall surface 312 intersects the virtual surface 317.
  • the mixing unit 80 extends downward on the right side of the end 315 and the inlet 306.
  • the mixing unit 80 is connected to the sample quantifying unit 114 via the passage 117.
  • the mixing unit 80 is connected to the reagent quantitative unit 134A via the first connection channel 301.
  • the mixing unit 80 is connected to the reagent quantification unit 134B via the second connection channel 331.
  • the sample 17A quantified in the sample quantification unit 114, the first reagent 18 quantified in the reagent quantification unit 134A, and the second reagent 19 quantified in the reagent quantification unit 134B are mixed.
  • the measurement light is transmitted to the measurement unit 81 that forms the lower part of the mixing unit 80.
  • the angle formed between the direction parallel to the reagent fixed amount surface 146 connecting the first connection portion 141 and the second connection portion 142 and the extending direction of the wall surface 132B on the first guide portion 138 side of the supply portion 132 is the first.
  • the angle formed by the direction parallel to the reagent fixed amount surface 146 and the extending direction of the second wall surface 308B is the second angle ⁇ 2.
  • the angle formed between the direction parallel to the reagent fixed amount surface 146 and the extending direction of the wall surface 137A of the second guide portion 137 connected to the second connection portion 142 is the third angle ⁇ 3.
  • the angle formed between the direction parallel to the reagent fixed surface 146 and the extending direction of the first wall surface 308A is the fourth angle ⁇ 4.
  • the angle formed by the direction parallel to the reagent fixed amount surface 146 and the wall surface 134C of the reagent fixed amount portion 134A connected to the first connection portion 141 is the fifth angle ⁇ 5.
  • a direction parallel to the reagent fixed amount surface 146 and a straight line 151 from the first connection portion 141 toward the first end portion 310 are formed.
  • the angle is the sixth angle ⁇ 6.
  • the third angle ⁇ 3 is formed to be larger than the first angle ⁇ 1. Further, the third angle ⁇ 3 is formed to be larger than the fourth angle ⁇ 4. Further, the second angle ⁇ 2 is formed to be larger than the third angle ⁇ 3. That is, each angle ⁇ 1 to ⁇ 4 satisfies the following relationship. 0 ⁇ first angle ⁇ 1 ⁇ third angle ⁇ 3 ⁇ second angle ⁇ 2 as well as, 0 ⁇ fourth angle ⁇ 4 ⁇ third angle ⁇ 3
  • the second angle Since ⁇ 2 is larger than the first angle ⁇ 1, the possibility that the first reagent 18 flows out from the first guide portion 138 to the mixing portion 80 side is reduced.
  • the first reagent 18 does not flow from the first guide portion 138 to the mixing portion 80 side, and the surplus first reagent 18 flows into the surplus portion 136A.
  • the second angle ⁇ 2 is larger than the fourth angle ⁇ 4, the second wall surface 308B of the first guide portion 138 that forms the second angle ⁇ 2 and the first wall surface 308A of the first guide portion 138 that forms the fourth angle ⁇ 4.
  • the first reagent 18 is retained. Since the retained first reagent 18 flows into the reagent quantification unit 143A when an external force such as centrifugal force or gravity is perpendicular to the reagent quantification surface 146, the quantification of the first reagent 18 in the reagent quantification unit 143A is performed. The possibility that the first reagent 18 is sometimes insufficient can be reduced.
  • the first reagent 18 contacts the first wall surface 308A when the first reagent 18 supplied to the supply unit 132 is injected into the reagent quantitative unit 143A. And even if it spreads, possibility that it will flow out to the mixing part 80 side by the 2nd wall surface 308B is reduced. Therefore, the possibility that the quantitative accuracy is lowered due to the shortage of the reagent and the possibility that the reagent flows out to the mixing unit 80 side is reduced, and the deterioration of the inspection accuracy is reduced.
  • the first reagent 18 is injected from the supply unit 132 into the reagent quantitative unit 134A through the third guide unit 139 along an external force such as centrifugal force or gravity.
  • the first reagent 18 overflowing from the reagent quantitative unit 134A is likely to flow into the surplus part 136A side along the wall surface 137A of the second guide part 137.
  • the third angle ⁇ 3 is larger than the fourth angle ⁇ 4, when the first reagent 18 is injected from the supply unit 132 along the external force such as centrifugal force or gravity into the reagent quantitative unit 134A via the third guide unit 139.
  • the first reagent 18 overflowing from the reagent quantitative unit 134A does not flow to the first guide unit 138 side, but easily flows into the surplus unit side 136A along the wall surface 137A of the second guide unit 137.
  • the fifth angle ⁇ 5 is formed to be larger than the sixth angle ⁇ 6. Therefore, when the first reagent 18 is injected from the supply unit 132 along the external force such as centrifugal force and gravity into the reagent fixed amount unit 134A via the third guide unit 139, the first reagent unit 138 holds the first reagent. When the amount of one reagent 18 increases, it flows more easily toward the reagent quantitative unit 134A than on the mixing unit 80 side. Thereby, the first reagent 18 held by the first guide unit 138 can be caused to flow into the reagent quantitative unit 134A.
  • the second end 140 which is the end of the third guide 139 closest to the reagent quantitative unit 134 ⁇ / b> A of the third guide 139, It is located on the opposite side of the reagent quantitative unit 134A from the straight line 152 connecting the one end 310.
  • the test chip 2 is rotated counterclockwise by an angle smaller than the first angle ⁇ 1, and the first force is generated by the centrifugal force.
  • the first reagent 18 flows out from the three guide portions 139, the flowed out first reagent 18 is stored in the first guide portion 138. Therefore, it is possible to suppress a decrease in inspection accuracy due to the outflow of the first reagent 18 from the first guide unit 138 to the mixing unit 80 side.
  • the flow path of the first guide portion 138 is wider than the second end portion 140 is on the same side as the reagent quantitative unit 134A than the straight line 152 connecting the second connection portion 142 and the first end portion 310. Accordingly, the first reagent 18 held by the second wall surface 308B of the first guide portion 138 that forms the second angle ⁇ 2 and the first wall surface 308A of the first guide portion 138 that forms the fourth angle ⁇ 4 is easy to move. Become.
  • the possibility that the external force such as centrifugal force or gravity will not flow into the reagent quantification unit 134 when it is perpendicular to the reagent quantification surface 146 is reduced, and the first reagent 18 quantified by the reagent quantification unit 134 is reduced.
  • the possibility that the other first reagents 18 will flow out to the mixing unit 80 side is reduced.
  • the test chip 2 includes a communication path 154 that extends in the extending direction from the reagent holding unit 131 toward the supply unit 132 and communicates the reagent holding unit 131 and the supply unit 132.
  • the third end portion 28 is an end portion of the third guide portion 139 opposite to the second end portion 140.
  • a virtual surface passing through the third end portion 28 is the inclined surface 150B.
  • the volume of the supply part 132 on the extending direction side of the communication path 154 from the inclined surface 150B, that is, the wall surface 132B side of the supply part 132 is the volume V2. As shown in FIG.
  • the volume of the supply portion 132 on the extending direction side of the imaginary plane 150A perpendicular to the extending direction at the end portion 26 in the extending direction of the communication passage 154, that is, on the right side shown in FIG. Is the volume V1.
  • the seventh angle ⁇ 7 formed by the inclined surface 150B in which the volume V2 is the same as the volume V1 and the direction parallel to the reagent quantitative surface 146 is formed smaller than the sixth angle ⁇ 6. .
  • the first reagent 18 As large as the volume V1 shown in FIG. 5A is supplied to the supply unit 132, in the process of injecting the first reagent 18 from the supply unit 132 to the reagent quantitative unit 134A, the first reagent 18 is perpendicular to the inclined surface 150B.
  • the first reagent 18 starts to be injected into the reagent quantification unit 134A via the third guide unit 139 from the time when the centrifugal force is directed in any direction. Since the sixth angle ⁇ 6 is larger than the seventh angle ⁇ 7 at the start of injection, there is a possibility that the injected first reagent 18 flows into the mixing unit 80 side beyond the first end 310 shown in FIG. Can be reduced. Therefore, it is possible to reduce a decrease in inspection accuracy.
  • the sixth angle ⁇ 6 is larger than the seventh angle ⁇ 7, so that the mixing unit exceeds the first end 310. The possibility of flowing into the 80 side is reduced.
  • the support shaft 46 extending from the L-shaped plate 60 is vertically connected to the center of the rear surface of the plate member 20 via a mounting holder (not shown). As the support shaft 46 rotates, the inspection chip 2 rotates around the support shaft 46.
  • the inspection chip 2 is in the steady state shown in FIGS. 2 and 3, the upper side 21 and the lower side 24 are orthogonal to the direction of gravity G, the right side 22 and the left side 23 are parallel to the direction of gravity G, and The left side portion 23 is disposed closer to the main shaft 57 than the right side portion 22.
  • the inspection apparatus 1 performs inspection by optical measurement by allowing the measurement light connecting the light source 71 and the optical sensor 72 to pass through the measurement unit 81.
  • Example of inspection method> An inspection method using the inspection apparatus 1 and the inspection chip 2 will be described.
  • the sample 17 is injected from the injection port 110 and placed in the sample holding unit 111.
  • the first reagent 18 is injected from the inlet 130 of the reagent quantitative flow path 13 and is disposed in the reagent holding part 131 of the reagent quantitative flow path 13.
  • the second reagent 19 is injected from the inlet 130 of the reagent quantitative channel 15 and is arranged in the reagent holding part 131 of the reagent quantitative channel 15.
  • the arrangement method of the first reagent 18, the second reagent 19, and the specimen 17 is not limited.
  • holes are opened at positions corresponding to the sample holding unit 111 and the reagent holding unit 131 in the sheets 291 and 292, and the user injects the sample 17, the first reagent 18, and the second reagent 19 from the holes, You may seal and seal.
  • the first reagent 18 and the second reagent 19 may be arranged in advance in the reagent holding portions 131 of the reagent quantitative flow paths 13 and 15 and sealed with sheets 291 and 292, respectively.
  • a hole may be opened in the sheet 291 at a position corresponding to the sample holding part 111 of the sample fixed amount flow path 11, and the user may inject the sample 17 from the hole, and further seal and seal.
  • the CPU 91 When the user attaches the inspection chip 2 to a mounting holder (not shown) and inputs a processing start command from the operation unit 94, the CPU 91 performs the centrifugal processing shown in FIG. 6 based on the control program stored in the ROM 93. Execute.
  • the inspection apparatus 1 can inspect two inspection chips 2 simultaneously, a procedure for inspecting one inspection chip 2 will be described below for convenience of explanation.
  • the steady state of the inspection chip 2 shown in FIGS. 2 and 3 is referred to as a rotation angle of 0 degree, and the state rotated 90 degrees counterclockwise from the steady state is referred to as a rotation angle of 90 degrees.
  • the inspection chip 2 rotates counterclockwise as viewed from the front.
  • the inspection chip 2 rotates clockwise as viewed from the front.
  • the CPU 91 reads motor drive information stored in advance in the HDD 95, sets drive information of the spindle motor 35 in the revolution controller 97, and sets drive information of the stepping motor 51 in the rotation controller 98. (S1). At this time, the test chip 2 is in a steady state and has a rotation angle of 0 degree as shown in FIGS. Next, the CPU 91 shown in FIG. 1 controls the revolution controller 97 to start driving the spindle motor 35 (S2). As a result, the inspection chip 2 having a rotation angle of 0 degrees revolves. The spindle motor 35 increases the rotation speed of the turntable 33 to the speed V based on an instruction from the revolution controller 97.
  • the speed V is, for example, 3000 rpm.
  • a centrifugal force X of several hundred G acts on the inspection chip 2.
  • the CPU 91 maintains the rotation speed of the spindle motor 35 at the speed V (S3).
  • the centrifugal force X acts on the test chip 2 from the left side 23 toward the right side 22.
  • the reagent 16 moves from the reagent holding unit 131 to the supply unit 132 by the action of the centrifugal force X, and the sample 17 moves from the sample holding unit 111 to the sample supply unit 112.
  • the rotation speed of the turntable 33 is assumed to be constant at the speed V, but the value of the speed V may be changed during the centrifugal process.
  • the CPU 91 controls the rotation controller 98 to drive and control the stepping motor 51 to rotate the inspection chip 2 up to a rotation angle of 90 degrees as shown in FIG. 7B (S4).
  • the centrifugal force X acts on the test chip 2 from the upper side portion 21 toward the lower side portion 24. Due to the action of the centrifugal force X, the reagent 16 flows from the supply unit 132 to the reagent quantitative unit 134 via the third guide unit 139, and the reagent 16 remaining in the reagent quantitative unit 134 passes through the second guide unit 137. It flows to the reagent surplus part 136.
  • the centrifugal force X acts in the direction perpendicular to the reagent fixed amount surface 146.
  • the capacity of the separation part 124 is the capacity of the liquid flow path 25 below the virtual surface 148 extending in the right direction from the end part 147 on the passage 125 side in the separation part 124 shown in FIG.
  • the first reagent 18 during the rotation of the test chip 2 from the rotation angle 0 degree shown in FIG. 7A to the rotation angle 90 degree shown in FIG. The flow will be described.
  • the first guide part 138 is first supplied from the supply part 132 via the third guide part 139.
  • Reagent 18 flows in. Since 2nd angle (theta) 2 is larger than 1st angle (theta) 1, possibility that the 1st reagent 18 will flow out from the 1st guide part 138 to the mixing part 80 side will be reduced.
  • the second angle ⁇ 2 is larger than the fourth angle ⁇ 4, the second wall surface 308B of the first guide portion 138 that forms the second angle ⁇ 2 and the first wall of the first guide portion 138 that forms the fourth angle ⁇ 4.
  • the first reagent 18 is held by the wall surface 308A.
  • the fifth angle ⁇ 5 is formed to be larger than the sixth angle ⁇ 6. Accordingly, the first reagent 18 held by the first guide unit 138 can easily flow to the reagent quantitative unit 134A side.
  • the sixth angle ⁇ 6 is larger than the seventh angle ⁇ 7, the first reagent 18 is supplied from the supply unit 132 via the third guide unit 139. The possibility that the first reagent 18 flowing out from the supply unit 132 flows out from the first guide unit 138 to the mixing unit 80 side when injected into the fixed amount unit 134A can be reduced.
  • the reagent 18 held in the first guide unit 138 flows from the first guide unit 138 into the reagent quantitative unit 134 as the rotation angle of the test chip 2 approaches 90 degrees. Since the second angle ⁇ 2 is larger than the third angle ⁇ 3, the first reagent 18 does not flow out from the first guide portion 138 to the mixing portion 80 side, and the surplus first reagent 18 flows into the surplus portion 136A. In addition, since the third angle ⁇ 3 is larger than the first angle ⁇ 1, the first reagent 18 overflowing from the reagent quantitative unit 134A can easily flow into the surplus part 136A along the wall surface 137A of the second guide part 137.
  • the first reagent 18 overflowing from the reagent quantitative unit 134A does not flow to the first guide unit 138 side, but along the wall surface 137A of the second guide unit 137. Thus, it becomes easy to flow into the surplus portion side 136A.
  • the CPU 91 holds the rotational speed of the spindle motor 35 at the speed V for a predetermined time (S5).
  • the centrifugal force X acts on the test chip 2 from the upper side portion 21 toward the lower side portion 24 for a predetermined time.
  • the separation unit 124 the components of the sample 17 are separated into the sample 17A and the sample 17B.
  • the specimen 17 is blood
  • blood cells having a large specific gravity accumulate on the side in which the centrifugal force X acts
  • plasma having a small specific gravity accumulates on the side opposite to the direction in which the centrifugal force X acts. That is, the specimen 17B, which is a blood cell in blood, and the specimen 17A, which is plasma, are separated.
  • the CPU 91 controls the rotation controller 98 to drive and control the stepping motor 51, and rotates the inspection chip 2 to the rotation angle of 0 degrees as shown in FIG. 8D (S6).
  • the centrifugal force X acts on the test chip 2 from the left side 23 toward the right side 22.
  • the first reagent 18 corresponding to the capacity of the partial receiving portion 304 remains in the partial receiving portion 304.
  • the first reagent 18 corresponding to the difference volume obtained by subtracting the volume of the partial receiving unit 304 from the volume of the reagent quantifying unit 134A is stored in the mixing unit 80.
  • the second reagent 19 quantified in the reagent quantification unit 134B moves to the reagent receiving unit 341.
  • the specimen 17A moves to the second supply unit 123 through the passage 127.
  • the sample 17A remaining in the separation unit 124 and a part of the sample 17B move to the component holding unit 121 via the connection channel 120.
  • the CPU 91 controls the rotation controller 98 to drive and control the stepping motor 51 to rotate the inspection chip 2 to a rotation angle of 90 degrees as shown in FIG. 8E (S7).
  • the centrifugal force X acts from the upper side portion 21 toward the lower side portion 24. Due to the action of the centrifugal force X, the specimen 17A flows from the second supply section 123 to the specimen quantification section 114 via the second guide section 128. The sample 17A remaining in the sample determination unit 114 flows to the second surplus unit 116 via the passage 115. The centrifugal force X acts in a direction perpendicular to the specimen quantification surface 129. Thereby, the sample 17A corresponding to the volume of the sample quantification unit 114 is quantified.
  • the first reagent 18 held in the partial receiver 304 moves to the reagent receiver 305.
  • the second reagent 19 held in the reagent receiving unit 341 moves to the reagent receiving unit 342.
  • the CPU 91 controls the rotation controller 98 to drive and control the stepping motor 51, and as shown in FIG. 8F, rotates the inspection chip 2 to the rotation angle of 0 degree (S8).
  • the centrifugal force X acts on the test chip 2 from the left side 23 toward the right side 22.
  • the centrifugal force X acts in the process of changing the posture of the inspection chip 2 from the state shown in FIG. 8E to the state shown in FIG. 8F and in the posture of the inspection chip 2 shown in FIG.
  • the first reagent 18 and the specimen 17A are mixed, and the first mixed liquid 261 is generated as shown in FIG.
  • the second reagent 19 moves from the reagent receiving part 342 to the reagent receiving part 343.
  • the CPU 91 controls the rotation controller 98 to drive and control the stepping motor 51 to rotate the inspection chip 2 to a rotation angle of 90 degrees as shown in FIG. 9G (S9).
  • the centrifugal force X acts on the inspection chip 2 from the upper side portion 21 toward the lower side portion 24.
  • the second reagent 19 moves from the reagent receiving part 343 to the reagent receiving part 344.
  • the second reagent 19 that has moved to the reagent receiving part 344 joins the first connection channel 301 formed on the front surface 201 via the joining hole part 351.
  • the CPU 91 controls the rotation controller 98 to drive and control the stepping motor 51 to rotate the inspection chip 2 to a rotation angle of 0 degrees as shown in FIG. 9H (S10).
  • the centrifugal force X acts on the test chip 2 from the left side 23 toward the right side 22.
  • the second reagent 19 moves to the mixing unit 80 and merges with the first mixed solution 261.
  • a second mixed liquid 262 in which the first reagent 18, the second reagent 19, and the specimen 17A are mixed is generated.
  • the CPU 91 controls the rotation controller 98 to drive and control the stepping motor 51 to rotate the inspection chip 2 to a rotation angle of 90 degrees as shown in FIG. 9I (S11).
  • the centrifugal force X acts on the inspection chip 2 from the upper side portion 21 toward the lower side portion 24. Due to the action of the centrifugal force X, the second mixed liquid 262 moves to the measuring unit 81.
  • the CPU 91 controls the rotation controller 98 to drive the stepping motor 51.
  • the CPU 91 rotates the inspection chip 2 until the rotation angle is 0 degree (S12).
  • the CPU 91 controls the revolution controller 97 to stop the rotation of the spindle motor 35 (S12). Therefore, the revolution of the inspection chip 2 is completed. Centrifugation is terminated.
  • the CPU 91 controls the revolution controller 97 to rotate and move the inspection chip 2 to the angle of the measurement position.
  • the measurement controller 99 shown in FIG. 1 causes the light source 71 to emit light
  • the measurement light passes through the second mixed liquid 262 stored in the measurement unit 81.
  • the CPU 91 performs optical measurement of the second liquid mixture 262 based on the change amount of the measurement light received by the optical sensor 72, and acquires measurement data.
  • CPU91 calculates the measurement result of the 2nd liquid mixture 262 based on the acquired measurement data.
  • the inspection result of the second mixed liquid 262 based on the measurement result is displayed on the display 96 shown in FIG.
  • the measuring method of the 2nd liquid mixture 262 is not restricted to an optical measurement, Another method may be sufficient.
  • the first reagent 18 is an example of the “reagent” of the present disclosure.
  • the second reagent 19 is an example of the “additional reagent” of the present disclosure.
  • the reagent quantification unit 134A is an example of the “quantification unit” of the present disclosure.
  • the reagent quantitative unit 134B is an example of the “quantitative unit” of the present disclosure.
  • the inclined surface 150B is an example of the “inclined surface” of the present disclosure.
  • the first reagent 18 may be changed to a specimen.
  • the measurement unit 81 is a lower part of the mixing unit 80, but may be provided separately from the mixing unit 80.
  • the reagent fixed amount flow channel 15 and the second connection flow channel 331 are formed on the rear surface 202 side, and the reagent fixed amount flow channel 13 and the first connection flow channel 301 are formed on the front surface 201, but are not limited thereto.
  • the reagent fixed amount flow path 15, the second connection flow path 331, the reagent fixed amount flow path 13, and the first connection flow path 301 may be formed on the front surface 201.
  • the first connection flow path 301 and the second connection flow path 331 may join at the flow path of the front surface 201 instead of joining at the joining hole portion 351.
  • the reagent quantitative flow channel 15 and the second connection flow channel 331 may not be provided in the test chip 2.
  • the reagent quantification unit 134 is a quantification unit that quantifies the reagent, but may be used as a sample quantification unit.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

La présente invention concerne une puce de contrôle qui améliore la précision de mesure. Un plan parallèle à un plan de mesure de réactif (146) qui relie une première partie de connexion (141) et une deuxième partie de connexion (142) forme un premier angle (θ1) à une première paroi latérale (132B) d'une partie de guidage (138) d'une partie d'alimentation (132), un autre plan parallèle au plan de mesure de réactif (146) forme un deuxième angle (θ2) avec une deuxième paroi (308B), un autre plan parallèle au plan de mesure de réactif (146) forme un troisième angle (θ3) avec une paroi (137A) d'une deuxième partie de guidage (137) qui relie la deuxième partie de connexion (142), et un autre plan parallèle au plan de mesure de réactif (146) forme un quatrième angle (θ4) avec une première paroi (308A). Lesdits angles satisfont aux relations suivantes : 0 < premier angle < troisième angle < deuxième angle ; et 0 < quatrième angle < troisième angle.
PCT/JP2014/081337 2013-11-29 2014-11-27 Puce de contrôle WO2015080192A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2013-248648 2013-11-29
JP2013248648A JP6028720B2 (ja) 2013-11-29 2013-11-29 検査チップ

Publications (1)

Publication Number Publication Date
WO2015080192A1 true WO2015080192A1 (fr) 2015-06-04

Family

ID=53199130

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2014/081337 WO2015080192A1 (fr) 2013-11-29 2014-11-27 Puce de contrôle

Country Status (2)

Country Link
JP (1) JP6028720B2 (fr)
WO (1) WO2015080192A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4112178A3 (fr) * 2020-07-22 2023-04-19 SKYLA Corporation Système et dispositif de détection biologique

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60238761A (ja) * 1984-05-03 1985-11-27 アボツト　ラボラトリーズ 化学的試験のための装置
JP2009156765A (ja) * 2007-12-27 2009-07-16 Rohm Co Ltd マイクロチップ
JP2012021854A (ja) * 2010-07-13 2012-02-02 Brother Ind Ltd 検査対象受体
JP2012202736A (ja) * 2011-03-24 2012-10-22 Brother Ind Ltd 検査対象受体、検査方法および検査装置

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60238761A (ja) * 1984-05-03 1985-11-27 アボツト　ラボラトリーズ 化学的試験のための装置
JP2009156765A (ja) * 2007-12-27 2009-07-16 Rohm Co Ltd マイクロチップ
JP2012021854A (ja) * 2010-07-13 2012-02-02 Brother Ind Ltd 検査対象受体
JP2012202736A (ja) * 2011-03-24 2012-10-22 Brother Ind Ltd 検査対象受体、検査方法および検査装置

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4112178A3 (fr) * 2020-07-22 2023-04-19 SKYLA Corporation Système et dispositif de détection biologique

Also Published As

Publication number Publication date
JP6028720B2 (ja) 2016-11-16
JP2015105890A (ja) 2015-06-08

Similar Documents

Publication Publication Date Title
JP6011156B2 (ja) 検査チップ
JP5998760B2 (ja) 試薬容器および検査チップ
JP6036666B2 (ja) 検査チップ
JP2014106207A (ja) 検査チップ
WO2014133125A1 (fr) Puce et système de test
JP5958452B2 (ja) 検査チップ
JP6028720B2 (ja) 検査チップ
JP5958330B2 (ja) 検査チップ
WO2014061635A1 (fr) Dispositif d&#39;inspection, système d&#39;inspection, procédé d&#39;inspection, et programme informatique
JP5915686B2 (ja) 検査チップ
JP5958451B2 (ja) 検査チップ、送液方法、及び送液プログラム
JP6137106B2 (ja) 検査チップ及び検査システム
JP5958331B2 (ja) 検査チップ及び検査システム
JP2015197352A (ja) 検査チップ
WO2015080191A1 (fr) Dispositif, procédé et programme d&#39;inspection
WO2014133126A1 (fr) Puce de test
WO2014061636A1 (fr) Puce d&#39;inspection
JP2015105888A (ja) 検査装置、検査方法、及び検査プログラム
JP5939148B2 (ja) 検査チップ及び検査システム
JP2015197351A (ja) 検査チップ
JP2016070808A (ja) 検査装置
JP2014119319A (ja) 検査チップ及び検査システム
JP2016070841A (ja) 検査チップ
JP2016188764A (ja) 検査チップ
JP5958249B2 (ja) 検査チップおよび検査装置

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 14865765

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 14865765

Country of ref document: EP

Kind code of ref document: A1