WO2015076368A1 - Procédé de préparation de sève, procédé de culture d'un micro-organisme, procédé de production d'une substance utile et procédé d'utilisation d'un composant de sève - Google Patents

Procédé de préparation de sève, procédé de culture d'un micro-organisme, procédé de production d'une substance utile et procédé d'utilisation d'un composant de sève Download PDF

Info

Publication number
WO2015076368A1
WO2015076368A1 PCT/JP2014/080895 JP2014080895W WO2015076368A1 WO 2015076368 A1 WO2015076368 A1 WO 2015076368A1 JP 2014080895 W JP2014080895 W JP 2014080895W WO 2015076368 A1 WO2015076368 A1 WO 2015076368A1
Authority
WO
WIPO (PCT)
Prior art keywords
sap
microbial fermentation
fermentation inhibitor
fermentation
preparing
Prior art date
Application number
PCT/JP2014/080895
Other languages
English (en)
Japanese (ja)
Inventor
昭彦 小杉
隆益 荒井
Original Assignee
独立行政法人国際農林水産業研究センター
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 独立行政法人国際農林水産業研究センター filed Critical 独立行政法人国際農林水産業研究センター
Publication of WO2015076368A1 publication Critical patent/WO2015076368A1/fr

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G, C10K; LIQUEFIED PETROLEUM GAS; ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE OR UNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL; FIRELIGHTERS
    • C10L1/00—Liquid carbonaceous fuels
    • C10L1/02—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L25/00—Food consisting mainly of nutmeat or seeds; Preparation or treatment thereof
    • A23L25/40—Fermented products; Products treated with microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/006—Waste from chemical processing of material, e.g. diestillation, roasting, cooking
    • C05F5/008—Waste from biochemical processing of material, e.g. fermentation, breweries
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00—Soil-conditioning materials or soil-stabilising materials
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00—Soil-conditioning materials or soil-stabilising materials
    • C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
    • C09K17/18—Prepolymers; Macromolecular compounds
    • C09K17/32—Prepolymers; Macromolecular compounds of natural origin, e.g. cellulosic materials
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/02—Pretreatment
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/02—Pretreatment
    • C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/06—Production of fats or fatty oils from raw materials by pressing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Definitions

  • the present invention relates to a method for preparing sap, a method for culturing microorganisms, a method for producing useful substances, and a method for utilizing sap components.
  • Palm oil is produced approximately 35.5 million tons / year in the world, but Malaysia and Indonesia account for approximately 87% of the production, and it is an agricultural product that represents Southeast Asia (2005) (According to yearly records and statistics from the US Department of Agriculture). Since palm oil is less expensive than soybean oil, it is used for foods such as margarine and fried oil, and is also widely used for industrial purposes such as soap and cosmetics.
  • Oil palm cultivated for palm oil production (oil palm, scientific name: Elaeis guineensis, Japanese name: oil palm) is required to be replanted every 20 to 25 years in order to maintain productivity .
  • oil palm scientific name: Elaeis guineensis, Japanese name: oil palm
  • In the case of Malaysia about 40,000 hectares of replanting is currently carried out by a full-fledged plantation from 1980, so about 30 million tons of palm trunks are logged annually. In the near future, it is expected that about 200,000-250,000 hectares of replanting will be required every year as a result of the expansion of plantation area.
  • Felled oil palm by replanting cultivation is caused by injecting drugs into the trunk and withering, or leaving or burning in the plantation after cutting, and there is concern that it may lead to serious environmental destruction. For this reason, there is a need for the development of a method for utilizing felled oil palm that does not place an environmental burden.
  • the oil palm trunk is composed of vascular bundles and fibers surrounding the vascular bundle. Therefore, the durability as wood is insufficient, and as a usage method, a relatively strong outer skin is used as a surface processing material such as plywood. Since other parts are unused and discarded, it is necessary to develop an effective use method for the inner part of the trunk as soon as possible.
  • Patent Document 1 includes a step of hydrolyzing residual fibers after microbial fermentation or / and squeezing in an oil palm stem juice with an enzyme and fermenting the obtained hydrolyzate together with microorganisms. Methods for producing ethanol and lactic acid containing are described.
  • the sap obtained from the oil palm trunk is recognized as a natural excellent medium, and ethanol can be produced by using it for ethanol fermentation using yeast.
  • the present inventors faced the problem that when fermentation using microorganisms such as bacteria is performed in a medium using sap obtained from oil palm trunk as a raw material, production failure and fermentation function decline occur.
  • This invention is made
  • the preparation method of the sap which can be used suitably for the culture medium suitable for culture
  • the method for preparing a sap of the present invention comprises a step of inactivating a microbial fermentation inhibitor in a sap obtained from a stem and / or petiole of oil palm, a step of separating the microbial fermentation inhibitor, and a microbial fermentation inhibitor.
  • a method for preparing a sap having one or more steps selected from the group consisting of steps to be removed.
  • the present invention provides one or more selected from the group consisting of the step of inactivating the microbial fermentation inhibitor, the step of separating the microbial fermentation inhibitor, and the step of removing the microbial fermentation inhibitor.
  • Is a method for preparing a sap in which the pH of the sap obtained from the stem and / or petiole of oil palm is adjusted to generate a precipitate.
  • the present invention provides one or more selected from the group consisting of the step of inactivating the microbial fermentation inhibitor, the step of separating the microbial fermentation inhibitor, and the step of removing the microbial fermentation inhibitor.
  • Is a method for preparing a sap in which the sap obtained from the trunk and / or petiole of oil palm is filtered with activated carbon or activated carbon is added to the sap.
  • 1 or 2 selected from the group consisting of the above-mentioned step of inactivating a microbial fermentation inhibitor, the step of separating the microbial fermentation inhibitor, and the step of removing the microbial fermentation inhibitor.
  • the above process is a sap preparation method in which a flocculant is added to the sap obtained from the stem and / or petiole of oil palm to generate an aggregate.
  • the present invention provides one or more selected from the group consisting of the step of inactivating the microbial fermentation inhibitor, the step of separating the microbial fermentation inhibitor, and the step of removing the microbial fermentation inhibitor.
  • One or two or more steps can be used.
  • the present invention provides one or more selected from the group consisting of the step of inactivating the microbial fermentation inhibitor, the step of separating the microbial fermentation inhibitor, and the step of removing the microbial fermentation inhibitor.
  • the microorganism cultivation method of the present invention is a microorganism cultivation method in which microorganisms are cultured using the sap prepared by the above-described sap preparation method.
  • the method for producing a useful substance of the present invention is a method for producing a useful substance by culturing a microorganism using the sap prepared by the above-described method for preparing a sap and producing the useful substance by the microorganism.
  • the present invention provides a step of recovering a microbial fermentation inhibitor that has been inactivated and / or removed by the above-described method for preparing a sap; and the recovered microbial fermentation inhibitor includes a fuel, a fuel auxiliary component, Food / beverage material, food / beverage aid, resin processing raw material, resin processing material, soil improvement material or fertilizer component added to fuel, food / beverage material, resin processing raw material, resin processing material, soil improvement material, or fertilizer A method for using the sap component.
  • cultivation of the microorganisms which used the sap obtained from the oil palm trunk as a raw material can be provided.
  • a composition other than the bark of a felled oil palm trunk that has been treated only as waste in the past can be used as a raw material to provide a medium suitable for microbial culture, as well as the resource value for the felled oil palm trunk. It is possible to establish a sustainable oil palm industry and reduce environmental impact.
  • FIG. 3 shows a precipitate obtained from weak alkali pH 9.0 treatment-sap.
  • the method for preparing a sap of this embodiment includes a step of inactivating a microbial fermentation inhibitor in a sap obtained from a stem and / or petiole of oil palm, a step of separating the microbial fermentation inhibitor, and the microbial fermentation inhibitor.
  • a method for preparing a sap having one or two or more steps selected from the group consisting of steps of removing sap.
  • the palm of the oil palm referred to in this specification includes a palm trunk that has fallen in fruit productivity, such as oil palm, sago palm, coconut palm, or nippa palm, or a palm trunk that is scheduled to be felled for more than 20 years. included.
  • the trunk of the oil palm may be a palm trunk that is cut for replanting cultivation or planned cultivation or a young palm trunk that is forced to be cut by a pest.
  • oil palm trunks include palm trunks that can be used to collect sap even after logging, such as palm trunks that have been peeled off and used for plywood applications.
  • oil palm petiole mentioned in the present specification is a petiole cut by a sickle or the like when collecting the fruit or bunch of oil palm trunk and collecting sap in the trunk.
  • oil palm leaf pattern may be a portion attached to the trunk when the trunk of the oil palm is cut.
  • a composition such as sap (hereinafter sometimes referred to as “sap”) from the trunk and / or petiole of oil palm.
  • physical compression, pulverization, drying, centrifugation, and water vapor are used.
  • the accompanying heating, hydration, organic solvent extraction methods can be used.
  • Squeezing can also be performed by the method described in Patent Document 3.
  • chemical treatment may be performed to facilitate collection of sap.
  • the method for preparing a sap of this embodiment includes a step of inactivating a microbial fermentation inhibitor in a sap obtained from a stem and / or petiole of oil palm, a step of separating the microbial fermentation inhibitor, and the microbial fermentation inhibitor. 1 or 2 or more processes selected from the group which consists of a process of removing.
  • the microbial fermentation inhibiting substance means a substance that inhibits the growth of microorganisms or reduces substance production efficiency in a fermentation process in which useful substances are produced by culturing microorganisms.
  • microbial fermentation inhibiting substance examples include, for example, a fermentation inhibiting substance contained in a sap obtained from the stem and / or petiole of oil palm immediately after pressing, and a composition such as sap from the stem and / or petiole of oil palm. Fermentation-inhibiting substances generated in the process of collecting
  • Examples of the microbial fermentation inhibitor in the present embodiment include phenolic substances and substances containing phenolic substances (hereinafter sometimes referred to as “phenol etc.”).
  • phenol and the like are not particularly limited as long as they are phenolic substances in the sap.
  • Monovalent phenols cresol, salicylic acid, picric acid, naphthol, etc.
  • divalent phenols catechol, resorcinol, hydroquinone, etc.
  • Trivalent phenols such as pyrogallol and phloroglucinol
  • hexavalent phenols such as hexahydroxybenzene
  • phenol and the like include aromatic ring compounds such as naphthalene rings and anthracene rings. Further, phenol and the like include a compound group in which a hydroxyl group, a carboxylic acid group or the like is bonded to an aromatic ring. Examples thereof include benzoic acids such as vanillic acid, hydroxybenzoic acid and dimethoxybenzoic acid, syringic acid, coumaric acid, ferulic acid and the like. In the present embodiment, all of these are collectively referred to as “total phenol”. In this embodiment, in addition to the total phenol in the sap, other substances that are thought to inhibit the productivity of useful substances may be removed.
  • the step of inactivating the microbial fermentation inhibitor contained in the sap obtained from the stem and / or petiole of oil palm, the step of separating the microbial fermentation inhibitor, and the step of removing the microbial fermentation inhibitor Although there is no particular limitation, as a specific embodiment, a method of adjusting the pH of the sap and generating a precipitate, a method of filtering the sap with activated carbon, or adding activated carbon to the sap, adding a flocculant to the sap 1 or 2 or more selected from the group consisting of a step of inactivating the polymer compound from the sap, a step of separating the polymer compound, or a step of removing the polymer compound A method including a process, a membrane filtration method, a treatment method using an adsorbent, a method for inactivating, separating or removing a fermentation inhibitor by heat sterilization such as an autoclave. Management methods, and the like. These methods may be used alone or in combination of one or more of these methods.
  • “inactivate the microbial fermentation inhibitor”, “separate the microbial fermentation inhibitor”, and “remove the microbial fermentation inhibitor” in the sap means, for example, that the microbial fermentation inhibitor is phenol.
  • the microbial fermentation inhibitor is phenol.
  • the total phenol concentration in the sap is preferably reduced by 40 to 100% by mass, more preferably 45 to 95% by mass before and after performing the specific operation as described above. .
  • the sap preparation method of this embodiment is a sap preparation method that adjusts the pH of the sap obtained from the trunk and / or petiole of oil palm and generates a precipitate.
  • the pH of the sap after collection is around 5.0 to 6.5, but by adjusting the pH of the sap, agglomeration occurs, and the microbial fermentation inhibitor in the sap is settled and separated to produce a precipitate.
  • the microbial fermentation inhibiting substance in the sap By precipitating the microbial fermentation inhibiting substance in the sap, the microbial fermentation inhibiting substance can be insolubilized. Thereby, it is considered that the microbial fermentative inhibitor can be separated or the reactivity can be largely lost.
  • the microbial fermentative inhibitor can be sequestered and / or inactivated.
  • the microbial fermentation inhibitor in a sap can be removed by removing the produced deposit.
  • the substance contained in the precipitate include the above-described phenols, but are not limited thereto, and proteins, heavy metals, metal ions, fine particles, and complex components via phenolic substances can be considered.
  • a growth inhibitory substance in the case of culturing a microorganism using a sap prepared by the sap preparation method of this embodiment or a fermentation inhibitor in the case of producing a useful substance can be mentioned.
  • the pH can be adjusted to 2.0 to 5.0 to generate a precipitate.
  • examples of the alkaline solution include sodium hydroxide, potassium hydroxide, calcium hydroxide, barium hydroxide, aqueous ammonia, copper hydroxide, aluminum hydroxide, iron hydroxide, ammonium hydroxide. , Sodium bicarbonate or an aqueous solution thereof.
  • the pH of the sap is adjusted to 8.0 to 12.0, preferably 9.0 to 11.0, thereby aggregating. Things can be precipitated.
  • a treatment such as stirring may be appropriately performed.
  • stirring it is preferable to carry out in a cool dark place.
  • the precipitate is solid-liquid separated to recover the solid component and the liquid component, and the precipitate can be removed.
  • Specific examples of the solid-liquid separation method for recovery or removal include a method using a centrifuge, a filter press, a screw press, a filter, a Laval separator, a precipitation tank, and the like.
  • the present invention can be implemented by one kind of solid-liquid separation method, two or more kinds may be used in combination, or a plurality may be combined as necessary. In this embodiment, it is preferable to carry out by centrifugation.
  • the pH may be adjusted again within the range of 5.0 to 5.5 using an acidic solution or an alkaline solution. Depending on the form to be used, the pH may not be adjusted.
  • the sap preparation method of this embodiment is a sap preparation method in which sap obtained from the trunk and / or petiole of oil palm is filtered with activated carbon, or activated carbon is added to and mixed with the sap.
  • phenol is adsorbed on the activated carbon.
  • the substance adsorbed on the activated carbon is not limited to phenol or the like, and as an example, when producing a growth inhibitory substance or useful substance when culturing microorganisms using sap prepared by the sap preparation method of this embodiment The fermentation inhibitory substance is mentioned.
  • the type of activated carbon used in the present embodiment is not particularly limited as long as it has the ability to reduce the total amount of growth inhibitory substances or fermentation inhibitory substances such as the above-mentioned phenols, but was obtained from oil palm residue. It is preferable that the activated carbon is prepared using fiber as a raw material.
  • the fiber obtained from the oil palm residue is obtained from the residue (also referred to as coconut shell or empty fruit bunch) generated in the oil palm oil extraction process, including the juice residue fiber obtained in the expression process. Refers to the fiber that has been produced.
  • activated carbon prepared using fiber obtained from oil palm juice residue as a raw material for the separation and removal of microbial fermentation inhibitors it is possible to effectively utilize oil palm residue that has been treated only as waste in the past it can.
  • activated carbon prepared using the fiber obtained from the oil palm residue as a raw material can be obtained.
  • the sap preparation method of the present embodiment is a sap preparation method in which a flocculant is added to a sap obtained from the trunk and / or petiole of oil palm to generate an aggregate.
  • a flocculant is added to a sap obtained from the trunk and / or petiole of oil palm to generate an aggregate.
  • an aggregating agent By adding an aggregating agent to the sap obtained from the stem and / or petiole of oil palm, an aggregating action occurs, and the microbial fermentation inhibitor in the sap can be separated by sedimentation to generate a precipitate.
  • the microbial fermentation inhibiting substance By precipitating the microbial fermentation inhibiting substance in the sap, the microbial fermentation inhibiting substance can be insolubilized. Thereby, it is considered that the microbial fermentative inhibitory substance can be isolated or the reactivity can be largely lost.
  • the microbial fermentative inhibitor can be sequestered and / or inactivated.
  • the microbial fermentation inhibitor in a sap can be removed by removing the produced deposit.
  • the flocculant used for this embodiment is not specifically limited, It is preferable that it is an inorganic flocculant.
  • inorganic flocculants include aluminum flocculants such as PAC (polyaluminum chloride) and sulfuric acid band (aluminum sulfate), and iron flocculants such as ferric chloride, but are not limited thereto. Absent.
  • the solid-liquid separation method include a method using a centrifugal separator, a filter press, a screw press, a filter, a Laval separator, a precipitation tank, and the like.
  • the present invention can be implemented by one kind of solid-liquid separation method, two or more kinds may be used in combination, or a plurality may be combined as necessary. In this embodiment, it is preferable to carry out by centrifugation.
  • the method for preparing a sap according to the present embodiment includes a step of inactivating a polymer compound from a sap obtained from the trunk and / or petiole of oil palm, a step of separating the polymer compound, and the polymer compound is removed.
  • This is a method for preparing a sap having one or more steps selected from the group consisting of steps.
  • examples of the method for removing the polymer compound include ultrafiltration and use of a dialysis membrane.
  • a high molecular compound is proteins, it can inactivate by the heating mentioned later.
  • the present embodiment it is preferable to have a step of removing the microbial fermentative inhibitor by using the above-described ultrafiltration, dialysis membrane or the like.
  • the sap preparation method of this embodiment is a sap method that includes a step of heating sap obtained from the trunk and / or petiole of oil palm. By performing the heat treatment, the fermentation inhibitor can be inactivated. By heating, mainly the phenolic compound in the sap or the polymer compound forming a complex with the phenolic compound is denatured and precipitates and aggregates.
  • the heating method is not particularly limited. For example, when heating is performed at 100 to 140 ° C., preferably 110 ° C. to 130 ° C. for 5 to 30 minutes, preferably 10 to 20 minutes, particularly preferably 5 to 10 minutes at one atmospheric pressure. Good. An autoclave or the like is preferably used for heating.
  • the heating temperature and the heating time may be appropriately adjusted as long as the fermentation inhibiting substance is inactivated.
  • it is also effective to heat at a low temperature of 50 ° C. to 80 ° C. for a long time.
  • the treatment time is preferably a short time, but depending on the case, a long time treatment may be used.
  • a pressure is generated with the heat treatment, but a sterilizing effect is also added by the pressure increase, so there is no problem in the treatment.
  • the purpose is achieved if the fermentation inhibiting substances in the sap are inactivated as precipitates or particles by these heat treatments.
  • due to heat treatment there are nutrient components in the sap, such as vitamins, which are vulnerable to heat, and microbial growth inhibition may occur. In this case, it can be improved by adding an appropriate amount of nutrients rich in vitamins such as yeast extract, polypeptone, meat extract, fish extract, corn steep liquor.
  • a microbial fermentation-inhibiting substance such as phenol contained in the sap is inactivated, separated, or removed, so that a useful substance using bacteria (for example, lactic acid which is a raw material of bioplastics).
  • a useful substance using bacteria for example, lactic acid which is a raw material of bioplastics.
  • PHB polyhydroxybutyric acid
  • biofuel butanol and the like
  • the sap that can be provided by the above sap preparation method can be suitably used as a medium suitable for culturing microorganisms.
  • the reason is guessed as follows. Palm sap has no hindrance to ethanol fermentation using yeast and can produce ethanol in theoretical yield. Therefore, it is recognized as a natural excellent medium without fermentation inhibition.
  • Non-patent paper 3 shows that the sap from the petiole contains the same components as the sap from the stem, and no fermentation inhibition is observed.
  • the present inventors have found problems such as production of lactic acid and PHB, particularly in the case of microbial fermentation using bacteria, such as poor growth and reduced fermentation ability. This cause was considered to be a phenomenon caused by a shortage of trace components such as vitamins in the sap.
  • the present inventors have found that phenolic substances inhibit the growth and fermentation of microorganisms as an example. Furthermore, it discovered that the total amount of growth inhibitory substances or fermentation inhibitory substances, such as a phenol contained in a sap, could be reduced by the alkali coagulation precipitation method mentioned above and the filtration method using activated carbon. Moreover, the preparation method of the sap of this invention can reduce the total amount of microbial fermentation inhibitors, such as a phenol, without reducing the amount of sugars in a sap. Therefore, the method for preparing a sap of the present invention can reduce the amount of a microbial fermentation inhibiting substance while suppressing the loss of fermentable free sugars in the sap. Since the above-mentioned method for preparing sap is obtained by reducing or removing substances considered to cause bacterial growth and fermentation inhibition, it is presumed that the method can be suitably used for a medium suitable for culturing microorganisms.
  • the microorganism culturing method of the present invention is a microorganism culturing method for culturing a microorganism using any sap prepared by the above preparation method.
  • the medium used in the culture method of the present invention includes any sap prepared by the above preparation method, and any medium such as a synthetic medium, a semi-synthetic medium, a natural medium, etc., as long as it is used for the growth of microorganisms. Can also be used.
  • a medium containing a carbon source and a nitrogen source is preferable.
  • As the carbon source glucose, fructose, maltose and sucrose are preferable.
  • As the nitrogen source amino acids and ammonium salts are preferable.
  • the eutrophic medium those containing yeast extract (yeast extra), proteolysates such as tryptone, peptone and polypeptone, and those derived from natural components such as wort are preferable.
  • yeast extract yeast extra
  • proteolysates such as tryptone, peptone and polypeptone
  • those derived from natural components such as wort are preferable.
  • Specific examples of the eutrophic medium include YPD medium, YEL medium, LB medium, and the like.
  • the medium may contain substances for promoting the growth of microorganisms such as vitamins, nucleic acids, metal salts, phosphorus, trace elements and the like.
  • microorganisms consume ethanol, acetic acid, etc. produced by metabolizing the carbon source as a carbon source.
  • the pH range in the microorganism culturing method of the present invention is not limited as long as it is within the range in which the microorganisms described later can grow, but is adjusted to, for example, 4 to 7.5, preferably 5 to 7.0.
  • acids such as hydrochloric acid, sulfuric acid, acetic acid and citric acid which are usually used as pH adjusting agents, alkalis such as sodium hydroxide, potassium hydroxide, calcium hydroxide, sodium hydrogen carbonate and ammonia, or Any of salts such as Tris hydrochloride, hydrogen phosphate, potassium hydrogen phosphate and the like can be used.
  • yeast extract when yeast extract is added, it may be prepared in a range of 0.01 to 2% by weight of yeast extract in 100% by weight of sap. If it is the range of this addition ratio, fermentation of microorganisms can be accelerated
  • microorganisms used during fermentation will be described.
  • microorganisms used in alcohol fermentation include Saccharomyces yeasts, Picea yeasts, Kluyveromyces yeasts, Zymomonas bacteria, Zymobacter bacteria, Clostridium bacteria, and the like.
  • Clostridium bacteria include Clostridium acetobutylicum, Clostridium beijerinckii, and Clostridium saccharobutyricum.
  • microorganisms used in lactic acid fermentation include Bacillus bacteria, Lactobacillus bacteria, Streptococcus bacteria, and Kluyveromyces yeasts.
  • Bacillus bacteria include Bacillus coagulans.
  • microorganisms used in the PHB fermentation include Comamonas bacteria, Lastonia bacteria, Barrioborax bacteria, Bacillus bacteria, and the like.
  • Bacillus bacteria include Bacillus megaterium. Not only these microorganisms but any microorganisms that can grow in the sap obtained by the present invention may be used.
  • microorganisms and fungi such as yeast, Escherichia coli, lactic acid bacteria, actinomycetes, fungi, mushrooms, etc. that have been genetically modified may be used, and by using these microorganisms, useful substances such as alcohols and lactic acid can be produced efficiently. be able to.
  • a microorganism that produces a hydrolase such as cellulase or amylase, for example, mold, Bacillus bacterium or Clostridium bacterium may be used in combination.
  • the culture tank for culturing the microorganism is not particularly limited.
  • the culture apparatus may have a function of keeping the temperature of the culture solution constant and stirring the culture solution. Since the optimal culture temperature varies depending on the type of microorganism, the purpose of culture, etc., the culture temperature can be determined as appropriate, but a culture temperature in the vicinity of 25 ° C. to 45 ° C. is preferred, and fermentation is carried out effectively in this temperature range. be able to. However, depending on the type of microorganism, it may be cultured and fermented in a low temperature range of 25 ° C. or lower or a high temperature range of 40 ° C. or higher. As other culture conditions, it is preferable to culture the microorganisms under the above-mentioned pH range, anaerobic conditions or aerobic conditions, depending on the microorganisms to be used.
  • the present invention it is possible not only to produce a useful substance such as alcohols or lactic acid at a high yield and low cost, but also to reduce the resource value for the felled palm trunk, using the felled palm trunk that has been treated only as waste as a raw material. It will be possible to establish a palm-related industry, including sustainable palm trunk treatment, and reduce the environmental burden.
  • the production method of the useful substance of the present invention is a method of culturing a microorganism using any sap prepared by the above preparation method and producing the useful substance by the microorganism.
  • a “useful substance” is a product obtained by fermentation of a nutrient source such as glucose by a microorganism.
  • useful substances include, but are not limited to, those that can be produced by microorganisms using glucose, such as C3-C5 lower alcohols such as ethanol and butanol; bioplastic plastic raw materials such as lactic acid and PHB; Materials targeted by refinery technology.
  • the fermentation performed in the present invention is not particularly limited, and alcohol fermentation, hydrogen fermentation, methane fermentation, organic acid fermentation such as lactic acid, succinic acid, and citric acid, 2,3-butanediol fermentation, acetoin fermentation, acetone.
  • microorganism examples include the above-described lactic acid bacteria when lactic acid is produced as a useful substance.
  • biopolymer-producing bacteria such as the above-mentioned Comamonas genus bacteria, Lastonia genus bacteria, Barrioborax bacteria, Bacillus genus bacteria and the like can be mentioned.
  • alcohol which has butanol as a main component as a useful substance butanol producing bacteria, such as the Clostridium bacterium mentioned above, are mentioned.
  • microorganisms for example, spring water, drainage, soil, etc. or other separation sources are mixed with water, if necessary, with hydrocarbon-containing substances such as crude oil and shaken in the water phase.
  • hydrocarbon-containing substances such as crude oil and shaken in the water phase.
  • Select a culture with increased strength due to the growth of microorganisms
  • What is necessary is just to select the strain which culture
  • the amino acid-producing bacteria include Brevibacterium, Corynebacterium, Microbacterium, Bacillus, or Escherichia.
  • the microorganism to which it belongs is mentioned.
  • L-amino acids include L-glutamic acid, L-lysine, L-glutamine, L-arginine, L-phenylalanine, L-threonine, L-isoleucine, L-histidine, L-proline, L-valine, L-serine, Examples include L-ornithine, L-citrulline, L-tyrosine, L-tryptophan and L-leucine.
  • D-amino acids examples include D-alanine, D-cysteine, D-aspartic acid, D-glutamic acid, D-phenylalanine, D-histidine, D-isoleucine, D-lysine, D-leucine, D-methionine, D -Asparagine, D-proline, D-glutamine, D-arginine, D-serine, D-threonine, D-valine, D-tryptophan, D-asparagine, D-tyrosine and the like.
  • the fermentation inhibitor containing inactivated and / or removed fermentation inhibitor produced by the sap preparation method or the microorganism cultured by the microorganism culture method is recovered, and the The microbial fermentation inhibiting substance can be added to fuel, food and drink materials, resin processed materials, resin processed materials, soil improvement materials, or fertilizers.
  • the collected fermentation inhibitor can be used as a resource.
  • phenolic substances containing a large amount of carbon are related to black liquor generated in the kraft pulp manufacturing process in Non-Patent Documents 4 and 5 as fuel components, and research on the conversion of contained phenolic substances to fuels is being conducted.
  • Non-patent documents 6 and 7 disclose that these phenolic compounds derived from natural plants have antioxidant activity and can be useful supplement materials in animals and humans. Recently, Non-Patent Document 8 reported that soil improvement was performed by including these phenolic compounds as fertilizer components. Further, as disclosed in Patent Document 9 as a promising use as a phenolic compound, it can be used as a resin raw material. Actually, as the wholly aromatic polyester, what is currently marketed is mainly composed of 4-hydroxybenzoic acid. As shown in the examples, 4-hydroxybenzoic acid is also used in the precipitate obtained by treating and separating the sap. Is included in the analysis results.
  • the microorganisms used were Bacillus coagrans, a thermophilic lactic acid fermenting bacterium, and Bacillus megaterium, an American type culture collection (ATCC), 11561 strain, which is a polyhydroxybutyric acid (PHB) producing bacterium as a bioplastic.
  • Bacillus coagulans was pre-cultured overnight using a medium for inoculating lactic acid bacteria (Nissui). The obtained sap was used to inoculate 0.5% by volume of the preculture.
  • 2 g ammonium sulfate, 2 g potassium dihydrogen phosphate, 2 g sodium chloride, 0.2 g magnesium sulfate, 0.05 g manganese sulfate, 0.01 g ferric sulfate, and 10 g yeast extract were added as nutrient sources. Sap was used.
  • a synthetic medium to be compared was prepared by adding glucose to the medium containing no sap so that glucose would be about 11.0% by weight (v / w).
  • the concentration of lactic acid accumulated in the culture solution was measured using a high performance liquid chromatography (HPLC) organic acid analysis system (Prominence, manufactured by Shimadzu Corporation) using a post-column pH buffered conductivity detection method.
  • HPLC high performance liquid chromatography
  • Bacillus megathorium ATCC 11561 strain which is a polyhydroxybutyric acid (PHB) producing bacterium
  • LB medium adjusted to pH 7.0 by dissolving 10 g bactopeptone, 10 g sodium chloride, 5 g yeast extract in 1 liter of distilled water.
  • sap (untreated) was directly inoculated with 0.5% by volume (v / v%).
  • a synthetic medium to be compared was prepared by adding glucose to the medium so as to be about 11.0% by weight (v / w).
  • the measurement of polyhydroxybutyric acid produced in the culture solution is performed by sampling from the culture solution every about 24 hours after inoculating the preculture solution, and after centrifugation (10,000 rotations, 4 ° C., 10 minutes), The supernatant was used as an analytical sample.
  • the measurement of lactic acid produced in the culture broth was carried out by inoculating the pre-culture broth, inoculating 1 ml from the broth approximately every 24 hours, centrifuging (10,000 rpm, 4 ° C., 5 min), After separation of the liquid and the precipitate, a precipitate fraction containing bacterial cells was used as an analysis sample.
  • the precipitate containing the cells was freeze-dried with a freeze-dryer (FDU-2100, manufactured by EYELA).
  • the polyhydroxybutyric acid concentration in the sample is qualitative by measuring the absorbance at UV 210 nm by high performance liquid chromatography (flow rate is 0.6 ml / min, 55 ° C.) using an Aminex HPX-87H (Bio-Rad Laboratories) column. Quantification was performed.
  • Clostridium saccharobutyricum was precultured in an anaerobic BUT medium.
  • the composition is adjusted to 5 g yeast extract, 10 g bactopeptone, 10 g powdered meat extract, 20 g soluble starch, 5 g sodium chloride, 0.5 g L-cysteine, 5 g glucose, 3 g sodium acetate, pH 7.0 per liter of distilled water. Thereafter, bubbling was performed with nitrogen gas to deaerate and replace with nitrogen to prepare a BUT medium. Using this BUT medium, stationary culture was performed overnight at 37 ° C. to prepare a preculture. The preculture was used to directly inoculate 0.5% by volume (v / v%) of sap.
  • a synthetic medium to be compared was prepared by adding glucose to the BUT medium so as to be about 11.0% by weight (v / w%).
  • the butanol produced in the culture solution is measured by sampling from the culture solution approximately every 24 hours after inoculating the preculture solution and centrifuging (10,000 rpm, 4 ° C., 5 minutes) to remove the supernatant.
  • the precipitate (including the bacterial cells) was separated, and the supernatant was used as an analysis sample.
  • the measurement was performed using gas chromatography (manufactured by Shimadzu Corporation, model BC-2014). Quantification was performed using n-propanol as an internal standard substance.
  • Corynebacterium glutamicus was first cultured in an amino acid fermentation medium.
  • the composition is 50 g of glucose, 30 g of ammonium sulfate, 1 g of KH2PO4, 0.4 g of MgSO4 ⁇ 7H2O, 0.01 g of FeSO4 ⁇ 7H2O, 0.01 g of MnSO4 ⁇ 5H2O, 200 ⁇ g of vitamin B1, 0.48 g of polypeptone, per liter of distilled water, After adjusting to pH 8.0 using 300 ⁇ g of biotin and potassium hydroxide, it was prepared by carrying out normal sterilization treatment by autoclave. After adding 1 g of calcium carbonate previously sterilized by dry heat, shaking culture was performed at 30 ° C.
  • Table 1 shows production amounts of fermentation tests using sap (no treatment).
  • a test inoculated into the synthetic medium used in the preculture without using sap as a subject was used as a comparative test. Since the sugar concentration in the sap (untreated) and the synthetic medium used in the pre-culture is different, glucose is added to the sap (untreated) so that the sap sugar concentration is 12.0% by mass, and then dissolved. did. As a result, when sap (untreated) was used, about half of lactic acid, the amount of bacterial cells serving as an index for accumulation of polyhydroxybutyric acid was hardly grown, and butanol production ability was hardly recognized.
  • Example 1 Treatment of sap with activated carbon
  • All the activated carbon was added so that it might become 5 mass% with respect to the said sap (non-processed) by dry weight.
  • Charcoal activated powder (Nacalai) marketed as activated carbon was used. After adding activated carbon, it was left at 4 ° C. for about 5 hours.
  • the activated carbon and the sap were separated by filtering the sap containing activated carbon, and the sap that passed through the filter was treated with activated carbon (activated carbon treatment-sap).
  • the activated carbon was prepared using the squeezed fiber which is a residue after oil palm squeezing. Carbonized at 600 ° C.
  • Example 2 Treatment of sap by pH adjustment
  • the normal pH of sap (untreated) is around 5.0 to 6.5.
  • 1N to 4N hydrochloric acid was added little by little and adjusted to pH 2.0, pH 3.0, pH 4.0, and pH 5.0, respectively.
  • 1N to 4N sodium hydroxide solution is added little by little, and pH 6.0, pH 7.0, pH 8.0, pH 9.0, pH 10.0, pH 11.0, pH 12.0, The pH was adjusted to 13.0.
  • the pH was adjusted by adding about 0.5 ml or less per 100 ml of sap. After adjustment, the mixture was allowed to stand at 4 ° C.
  • saps are treated as pH-adjusted sap and have an acidic pH of pH 2.0-4.0 (acidic pH treatment-sap), a neutral pH of 5.0-7.0 (neutral pH treatment-sap), pH 8 0.0-11.0 weak alkali pH (weak alkali pH treatment-sap) and strong alkali pH of pH 12.0-13.0 (strong alkali pH treatment-sap) were examined for their tendency to separate.
  • Example 3 Treatment of sap with flocculant
  • the treatment was performed with an inorganic flocculant.
  • Aluminum sulfate and polyaluminum chloride were used as inorganic flocculants.
  • 0.2 wt% (w / v) was added to each 1 liter of sap (untreated), and a precipitate was generated by allowing to stand at 4 ° C. for 1 hour. Thereafter, centrifugation was performed in the same manner as described above to remove the precipitate.
  • the obtained sap was used in experiments as a sap treated with a flocculant.
  • Example 4 Treatment of sap by ultrafiltration
  • the sap was treated using an ultrafiltration membrane.
  • the sap was centrifugally filtered using a centrifugal ultrafiltration device as the ultrafiltration membrane.
  • About 30 ml of sap (untreated) is used, and compounds with a molecular weight of 10,000 or less are contained by centrifugation at 5,000 rpm for 30 minutes using a centrifugal ultrafiltration membrane device (Millipore Amicon, molecular weight 10,000 cut).
  • a filtrate was prepared. The filtrate was used in the experiment as an ultrafiltration treatment (low molecular fraction-sap).
  • Example 5 Treatment of sap by heat treatment
  • heat treatment was performed by autoclave. About 50 ml of sap was transferred to a glass Erlenmeyer flask and pretreated at 120 ° C. for 10 minutes using an autoclave manufactured by Tommy Seiko. The prepared sap produced an insoluble precipitate such as protein, but was used as it was.
  • Example 6 Fermentation test using pretreated sap
  • various pretreated sap Examples 1 to 5, Comparative Example 1
  • Bacillus coagulans Bacillus coagulans
  • a thermophilic lactic acid fermentation bacterium Bacillus mega
  • Bacillus mega a bioplastic polyhydroxybutyric acid-producing bacterium
  • a fermentation test was performed using thorium, ATCC 11561 strain, and Clostridium saccharobutyricum NBRC109358, which is a biobutanol-producing bacterium.
  • the results are shown in Table 3. From the results, it became clear that the fermentation production efficiency of the sap after each treatment was improved by about 1.2 to 2.6 times compared to the untreated sap.
  • Example 7 Effect on amino acid fermentation production by pretreatment of oil palm stem juice liquid Among amino acid fermentations, glutamic acid is known as representative amino acid fermentation production.
  • sap pretreatment sap treated with activated carbon treatment, weak alkaline pH treatment (pH 11.0), and flocculant (polyaluminum chloride) ( A glutamic acid fermentation test was conducted using Examples 1 to 3 and Comparative Example 1). The results are shown in Table 5. As a result, since the production ability of glutamic acid was improved as compared with the untreated sap, the influence of the sap pretreatment was confirmed in amino acid fermentation.
  • the fermentative capacity of the sap pretreated from the commercially available synthetic medium shown in Table 1 is clear. Very expensive. It is clear that this can be used as a fermentation production medium above the synthetic medium by inactivating the phenolic substance assumed to be a fermentation inhibitory substance by precipitation, or fractionating and removing it, in the sap that squeezed the oil palm trunk. It became.
  • Example 9 The agglomerated precipitate of fermentation inhibitor containing phenolic substance obtained by weak alkaline pH treatment-sap described in Example 2 above was collected by centrifugation (10,000 rpm, 4 ° C., 10 minutes). Moreover, the activated carbon used for the activated carbon treated sap was separated and recovered by a Whatman filter. These precipitates and activated carbon were dried overnight at 70 ° C. in a thermostatic oven. This aspect is shown in FIGS. 1 and 2, respectively. As shown in Table 2 above, these precipitates or activated carbon were considered to contain phenolic compounds and fiber-derived fine particles.
  • phenolic substances containing a large amount of carbon include fuel components (Non-patent documents 4 and 5), useful supplement materials (Non-patent documents 6 and 7), fertilizer components (Non-patent document 8: Tea polyphenol-containing organic fertilizer production technology) It is known that the solar eclipse 1995/11/15 date 07959 No. 02 surface A), or a resin material (Patent Document 9) can be used.
  • GC / MS uses GC6890 (Agilent Technologies) and AutospecUltima (Micromass), column DB-5MS, carrier gas is helium (2 mL / min) heating program: 60 ° C (1 min) 5 ° C / min 320 ° C (7 min) The analysis was performed.
  • organic acids such as glycerol, citric acid, succinic acid, fumaric acid and the like, which have an effect of promoting various microorganisms, were recognized in the range of about 49 ⁇ g to 250 ⁇ g in 1 ml of sap.
  • substances such as vanillic acid and syringacic acid, such as parahydroxybenzoic acid and dimethylbenzoic acid, which are considered to have an aromatic ring, are contained in a range of about 30 ⁇ g to 260 ⁇ g in 1 ml of sap.
  • FIG. 3 shows a flow of sap use according to the present invention and microbial fermentation inhibitory substance obtained from sap.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Soil Sciences (AREA)
  • General Life Sciences & Earth Sciences (AREA)
  • Materials Engineering (AREA)
  • Polymers & Plastics (AREA)
  • Botany (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Environmental & Geological Engineering (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fertilizers (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Liquid Carbonaceous Fuels (AREA)
  • Soil Conditioners And Soil-Stabilizing Materials (AREA)

Abstract

L'invention concerne un procédé de préparation d'une sève, ladite sève étant utilisable de façon appropriée dans un milieu adapté pour la culture d'un micro-organisme, au moyen d'une sève obtenue à partir de troncs et fanes de palmiers à huile en tant que matière de départ. Le procédé de préparation d'une sève selon la présente invention est caractérisé en ce qu'il comprend une ou plusieurs étapes choisies dans le groupe constitué d'une étape d'inactivation d'inhibiteur(s) de fermentation contenu(s) dans une sève obtenue à partir de troncs et de fanes de palmiers à huile, une étape pour leur séparation et une étape pour leur retrait. Au moyen de la sève, la fermentation microbienne peut être davantage favorisée de sorte qu'une substance utile peut être produite à un plus haut rendement. Dans la présente invention, des exemples d'inhibiteur(s) de fermentation microbienne comprennent les substances phénoliques ou les substances contenant des substances phénoliques.
PCT/JP2014/080895 2013-11-21 2014-11-21 Procédé de préparation de sève, procédé de culture d'un micro-organisme, procédé de production d'une substance utile et procédé d'utilisation d'un composant de sève WO2015076368A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2013-241175 2013-11-21
JP2013241175A JP5825690B2 (ja) 2013-11-21 2013-11-21 樹液の調製方法、微生物の培養方法、有用物質の生産方法及び樹液成分の活用方法

Publications (1)

Publication Number Publication Date
WO2015076368A1 true WO2015076368A1 (fr) 2015-05-28

Family

ID=53179627

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2014/080895 WO2015076368A1 (fr) 2013-11-21 2014-11-21 Procédé de préparation de sève, procédé de culture d'un micro-organisme, procédé de production d'une substance utile et procédé d'utilisation d'un composant de sève

Country Status (3)

Country Link
JP (1) JP5825690B2 (fr)
MY (1) MY173672A (fr)
WO (1) WO2015076368A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020255900A1 (fr) * 2019-06-17 2020-12-24 国立研究開発法人国際農林水産業研究センター Procédé de culture de microalgue

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008090707A1 (fr) * 2007-01-25 2008-07-31 Japan International Research Center For Agricultural Sciences Méthode de production d'éthanol ou d'acide lactique

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008090707A1 (fr) * 2007-01-25 2008-07-31 Japan International Research Center For Agricultural Sciences Méthode de production d'éthanol ou d'acide lactique

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
AKIHIKO KOBAYASHI ET AL.: "Bassai Oil Palm Kan no Jueki o Mochiita Ethanol Hakko Shiken", JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY TAIKAI KOEN YOSHISHU 2009 NENDO (HEISEI 21 NENDO) TAIKAI, JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, 5 March 2009 (2009-03-05), pages 336 *
CHOOKLIN, S. ET AL.: "Potential use of Lactobacillus casei TISTR 1500 for the bioconversion from palmyra sap and oil palm sap to lactic acid", ELECTRONIC JOURNAL OF BIOTECHNOLOGY, vol. 14, no. 5, 15 September 2011 (2011-09-15) *
KOMONKIAT, I . ET AL.: "Felled oil palm trunk as a renewable source for biobutanol production by Clostridium spp", BIORESOURCE TECHNOLOGY, vol. 146, 22 July 2013 (2013-07-22), pages 200 - 207 *
KOSUGI, A. ET AL.: "Ethanol and lactic acid production using sap squeezed from old oil palm trunks felled for replanting", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 110, no. 3, 25 March 2010 (2010-03-25), pages 322 - 325 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020255900A1 (fr) * 2019-06-17 2020-12-24 国立研究開発法人国際農林水産業研究センター Procédé de culture de microalgue

Also Published As

Publication number Publication date
JP5825690B2 (ja) 2015-12-02
JP2015100283A (ja) 2015-06-04
MY173672A (en) 2020-02-14

Similar Documents

Publication Publication Date Title
Bonilla-Hermosa et al. Utilization of coffee by-products obtained from semi-washed process for production of value-added compounds
Zahari et al. Renewable sugars from oil palm frond juice as an alternative novel fermentation feedstock for value-added products
Cerrone et al. Use of a mannitol rich ensiled grass press juice (EGPJ) as a sole carbon source for polyhydroxyalkanoates (PHAs) production through high cell density cultivation
JP4065960B1 (ja) エタノール及び乳酸の製造方法
Camargo et al. Xylitol bioproduction in hemicellulosic hydrolysate obtained from sorghum forage biomass
Tsouko et al. Valorization of by-products from palm oil mills for the production of generic fermentation media for microbial oil synthesis
Salgado et al. Purification of ferulic acid solubilized from agroindustrial wastes and further conversion into 4-vinyl guaiacol by Streptomyces setonii using solid state fermentation
Hamouda et al. Bioethanol production by various hydrolysis and fermentation processes with micro and macro green algae
CN109486876A (zh) 一种发酵、提取和纯化苏氨酸的方法
Al-Shorgani et al. The use of pretreated palm oil mill effluent for acetone–butanol–ethanol fermentation by Clostridium saccharoperbutylacetonicum N1-4
Wadi et al. Production of bioethanol from seaweed, Gracilaria verrucosa and Eucheuma cottonii, by simultaneous saccharification and fermentation methods
CN106278493A (zh) 分级酶解法制备含寡糖海藻有机肥的方法
Pattnaik et al. Production of phenolic flavoring compounds from sugarcane bagasse by Lactobacillus acidophilus MTCC 10307
EP2604696A1 (fr) Procédé de production par fermentation d'acide lactique à partir d'un extrait de plante en présence d'un sel de magnésium caustique
Gui et al. Fungus-assisted mild acid pretreatment of Glycyrrhiza uralensis residues to enhance enzymatic hydrolysis and oil production by green microalga Chlorella protothecoides
Duarte et al. Yeast biomass production in brewery’s spent grains hemicellulosic hydrolyzate
Dien et al. Conversion of SPORL pretreated Douglas fir forest residues into microbial lipids with oleaginous yeasts
Azam et al. Date palm waste: an efficient source for production of glucose and lactic acid
WO2007083746A1 (fr) Méthode de fermation pour la production d'éthanol
JP5825690B2 (ja) 樹液の調製方法、微生物の培養方法、有用物質の生産方法及び樹液成分の活用方法
Szambelan et al. The composition of Jerusalem artichoke (Helianthus tuberosus L.) spirits obtained from fermentation with bacteria and yeasts
Koutinas et al. Production of fermentation feedstock from Jerusalem artichoke tubers and its potential for Polyhydroxybutyrate synthesis
JP6238416B2 (ja) 樹液の調製方法
Volkmar et al. Municipal green waste as substrate for the microbial production of platform chemicals
CN101497902B (zh) 一种微生物油脂的制备方法

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 14863196

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: IDP00201603346

Country of ref document: ID

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 14863196

Country of ref document: EP

Kind code of ref document: A1