WO2015073163A3 - Synthesis and enrichment of nucleic acid sequences - Google Patents

Synthesis and enrichment of nucleic acid sequences Download PDF

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Publication number
WO2015073163A3
WO2015073163A3 PCT/US2014/061435 US2014061435W WO2015073163A3 WO 2015073163 A3 WO2015073163 A3 WO 2015073163A3 US 2014061435 W US2014061435 W US 2014061435W WO 2015073163 A3 WO2015073163 A3 WO 2015073163A3
Authority
WO
WIPO (PCT)
Prior art keywords
enrichment
nucleic acid
acid sequences
synthesis
target
Prior art date
Application number
PCT/US2014/061435
Other languages
French (fr)
Other versions
WO2015073163A2 (en
WO2015073163A8 (en
Inventor
Jason Poole
Saege HANCOCK
Karena Kosco
Vlada MELNIKOVA
Peter CROUCHER
Tim Lu
Mark G. Erlander
Errin SAMUELSZ
Original Assignee
Trovagene, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Trovagene, Inc. filed Critical Trovagene, Inc.
Priority to CN201480013111.4A priority Critical patent/CN105283555A/en
Priority to EP14861883.8A priority patent/EP2951321A4/en
Priority to CA2922261A priority patent/CA2922261A1/en
Priority to JP2016524530A priority patent/JP2016535987A/en
Priority to US15/027,688 priority patent/US20160273022A1/en
Publication of WO2015073163A2 publication Critical patent/WO2015073163A2/en
Publication of WO2015073163A3 publication Critical patent/WO2015073163A3/en
Publication of WO2015073163A8 publication Critical patent/WO2015073163A8/en
Priority to HK15112569.9A priority patent/HK1211628A1/en
Priority to US15/256,523 priority patent/US9725755B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/118—Prognosis of disease development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present disclosure relates to the enrichment of target nucleic acid sequences present in low-abundance relative to corresponding non-target or reference nucleic acid sequence in a sample. In particular, the methods allow for a substantially greater level of detection sensitivity of target sequence by orders of magnitude enrichment of a low-abundance sequence.
PCT/US2014/061435 2013-10-20 2014-10-20 Synthesis and enrichment of nucleic acid sequences WO2015073163A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CN201480013111.4A CN105283555A (en) 2013-10-20 2014-10-20 Synthesis and enrichment of nucleic acid sequences
EP14861883.8A EP2951321A4 (en) 2013-10-20 2014-10-20 Synthesis and enrichment of nucleic acid sequences
CA2922261A CA2922261A1 (en) 2013-10-20 2014-10-20 Synthesis and enrichment of nucleic acid sequences
JP2016524530A JP2016535987A (en) 2013-10-20 2014-10-20 Synthesis and enrichment of nucleic acid sequences
US15/027,688 US20160273022A1 (en) 2013-10-20 2014-10-20 Synthesis and enrichment of nucleic acid sequences
HK15112569.9A HK1211628A1 (en) 2013-11-14 2015-12-21 Synthesis and enrichment of nucleic acid sequences
US15/256,523 US9725755B2 (en) 2013-10-20 2016-09-03 Synthesis and enrichment of nucleic acid sequences

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201361904141P 2013-11-14 2013-11-14
US61/904,141 2013-11-14
US201462039905P 2014-08-20 2014-08-20
US62/039,905 2014-08-20
US201461893283P 2014-10-20 2014-10-20
US61/893,283 2014-10-20

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US15/027,688 A-371-Of-International US20160273022A1 (en) 2013-10-20 2014-10-20 Synthesis and enrichment of nucleic acid sequences
US15/256,523 Continuation US9725755B2 (en) 2013-10-20 2016-09-03 Synthesis and enrichment of nucleic acid sequences

Publications (3)

Publication Number Publication Date
WO2015073163A2 WO2015073163A2 (en) 2015-05-21
WO2015073163A3 true WO2015073163A3 (en) 2015-11-05
WO2015073163A8 WO2015073163A8 (en) 2015-12-17

Family

ID=53058234

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2014/061435 WO2015073163A2 (en) 2013-10-20 2014-10-20 Synthesis and enrichment of nucleic acid sequences

Country Status (2)

Country Link
CA (1) CA2922261A1 (en)
WO (1) WO2015073163A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107604053A (en) * 2017-08-16 2018-01-19 金绍莲 Application and its kit and detection method of a kind of wild-type amplification blocker in detection gene trace mutation reagent is prepared
US20220348606A1 (en) * 2019-06-26 2022-11-03 Nanjing GenScript Biotech Co., Ltd. Oligonucleotide containing blocker
CN112375808A (en) * 2020-11-18 2021-02-19 合肥欧创基因生物科技有限公司 Blocker design and screening method of ARMS-TaqMan Blocker system
CN117025765A (en) * 2023-02-01 2023-11-10 珠海圣美生物诊断技术有限公司 Multiplex digital PCR detection kit and detection method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020098510A1 (en) * 2000-12-14 2002-07-25 Affymetrix, Inc. Blocker-aided target amplification of nucleic acids
US20100203532A1 (en) * 2007-08-01 2010-08-12 Dana-Farber Cancer Institute Enrichment of a Target Sequence
US20110217714A1 (en) * 2010-03-08 2011-09-08 Dana-Farber Cancer Institute, Inc. Full Cold-PCR Enrichment with Reference Blocking Sequence

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020098510A1 (en) * 2000-12-14 2002-07-25 Affymetrix, Inc. Blocker-aided target amplification of nucleic acids
US20100203532A1 (en) * 2007-08-01 2010-08-12 Dana-Farber Cancer Institute Enrichment of a Target Sequence
US20110217714A1 (en) * 2010-03-08 2011-09-08 Dana-Farber Cancer Institute, Inc. Full Cold-PCR Enrichment with Reference Blocking Sequence

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2951321A4 *

Also Published As

Publication number Publication date
CA2922261A1 (en) 2015-05-21
WO2015073163A2 (en) 2015-05-21
WO2015073163A8 (en) 2015-12-17

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