WO2015073163A8 - Synthesis and enrichment of nucleic acid sequences - Google Patents

Synthesis and enrichment of nucleic acid sequences Download PDF

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Publication number
WO2015073163A8
WO2015073163A8 PCT/US2014/061435 US2014061435W WO2015073163A8 WO 2015073163 A8 WO2015073163 A8 WO 2015073163A8 US 2014061435 W US2014061435 W US 2014061435W WO 2015073163 A8 WO2015073163 A8 WO 2015073163A8
Authority
WO
WIPO (PCT)
Prior art keywords
enrichment
nucleic acid
acid sequences
synthesis
target
Prior art date
Application number
PCT/US2014/061435
Other languages
French (fr)
Other versions
WO2015073163A2 (en
WO2015073163A3 (en
Inventor
Jason Poole
Saege HANCOCK
Karena Kosco
Vlada MELNIKOVA
Peter CROUCHER
Tim Lu
Mark G. Erlander
Errin SAMUELSZ
Original Assignee
Trovagene, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US201361904141P priority Critical
Priority to US61/904,141 priority
Priority to US201462039905P priority
Priority to US62/039,905 priority
Application filed by Trovagene, Inc. filed Critical Trovagene, Inc.
Priority to US201461893283P priority
Priority to US61/893,283 priority
Priority claimed from EP14861883.8A external-priority patent/EP2951321A4/en
Priority claimed from CN201480013111.4A external-priority patent/CN105283555A/en
Publication of WO2015073163A2 publication Critical patent/WO2015073163A2/en
Publication of WO2015073163A3 publication Critical patent/WO2015073163A3/en
Publication of WO2015073163A8 publication Critical patent/WO2015073163A8/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/118Prognosis of disease development
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers

Abstract

The present disclosure relates to the enrichment of target nucleic acid sequences present in low-abundance relative to corresponding non-target or reference nucleic acid sequence in a sample. In particular, the methods allow for a substantially greater level of detection sensitivity of target sequence by orders of magnitude enrichment of a low-abundance sequence.
PCT/US2014/061435 2013-11-14 2014-10-20 Synthesis and enrichment of nucleic acid sequences WO2015073163A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
US201361904141P true 2013-11-14 2013-11-14
US61/904,141 2013-11-14
US201462039905P true 2014-08-20 2014-08-20
US62/039,905 2014-08-20
US201461893283P true 2014-10-20 2014-10-20
US61/893,283 2014-10-20

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
EP14861883.8A EP2951321A4 (en) 2013-10-20 2014-10-20 Synthesis and enrichment of nucleic acid sequences
CN201480013111.4A CN105283555A (en) 2013-10-20 2014-10-20 Synthesis and enrichment of nucleic acid sequences
US15/027,688 US20160273022A1 (en) 2013-10-20 2014-10-20 Synthesis and enrichment of nucleic acid sequences
JP2016524530A JP2016535987A (en) 2013-10-20 2014-10-20 Synthesis and enrichment of nucleic acid sequences
CA2922261A CA2922261A1 (en) 2013-10-20 2014-10-20 Synthesis and enrichment of nucleic acid sequences
HK15112569.9A HK1211628A1 (en) 2013-11-14 2015-12-21 Synthesis and enrichment of nucleic acid sequences
US15/256,523 US9725755B2 (en) 2013-10-20 2016-09-03 Synthesis and enrichment of nucleic acid sequences

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US15/027,688 A-371-Of-International US20160273022A1 (en) 2013-10-20 2014-10-20 Synthesis and enrichment of nucleic acid sequences
US201615027688A A-371-Of-International 2016-04-06 2016-04-06
US15/256,523 Continuation US9725755B2 (en) 2013-10-20 2016-09-03 Synthesis and enrichment of nucleic acid sequences

Publications (3)

Publication Number Publication Date
WO2015073163A2 WO2015073163A2 (en) 2015-05-21
WO2015073163A3 WO2015073163A3 (en) 2015-11-05
WO2015073163A8 true WO2015073163A8 (en) 2015-12-17

Family

ID=53058234

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2014/061435 WO2015073163A2 (en) 2013-11-14 2014-10-20 Synthesis and enrichment of nucleic acid sequences

Country Status (2)

Country Link
CA (1) CA2922261A1 (en)
WO (1) WO2015073163A2 (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6391592B1 (en) * 2000-12-14 2002-05-21 Affymetrix, Inc. Blocker-aided target amplification of nucleic acids
AU2008282780B2 (en) * 2007-08-01 2014-04-17 Dana- Farber Cancer Institute Enrichment of a target sequence
EP2545189B1 (en) * 2010-03-08 2018-01-10 Dana-Farber Cancer Institute, Inc. Full cold-pcr enrichment with reference blocking sequence

Also Published As

Publication number Publication date
WO2015073163A3 (en) 2015-11-05
WO2015073163A2 (en) 2015-05-21
CA2922261A1 (en) 2015-05-21

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