WO2015070748A1 - Carte à bâtonnet diagnostique - Google Patents

Carte à bâtonnet diagnostique Download PDF

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Publication number
WO2015070748A1
WO2015070748A1 PCT/CN2014/090851 CN2014090851W WO2015070748A1 WO 2015070748 A1 WO2015070748 A1 WO 2015070748A1 CN 2014090851 W CN2014090851 W CN 2014090851W WO 2015070748 A1 WO2015070748 A1 WO 2015070748A1
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WO
WIPO (PCT)
Prior art keywords
test strip
sample
test
strip
cartridge
Prior art date
Application number
PCT/CN2014/090851
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English (en)
Chinese (zh)
Inventor
马义才
顾敏
马灵
Original Assignee
成都领御生物技术有限公司
马义才
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by 成都领御生物技术有限公司, 马义才 filed Critical 成都领御生物技术有限公司
Publication of WO2015070748A1 publication Critical patent/WO2015070748A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases

Definitions

  • the invention belongs to the field of in vitro diagnosis, and particularly relates to a test information capable of filtering a sample by itself, storing a standard curve or a coefficient parameter of a test object with a storage medium 14 , and combining the instrument with the signal detection function to quickly detect a single sample Component or multi-component strip card.
  • Immunochromatographic strip technology which is mainly represented by colloidal gold strips, has been widely used in various aspects of immunoassays.
  • the technology is simple and fast, and results are available in minutes.
  • this technology has the following shortcomings:
  • red blood cells in the whole blood sample will prevent the smooth migration of the reactants on the test strip.
  • the red blood cell color (red) is similar to or the same as the colloidal gold display color (light red or purple), which may interfere with the detection result judgment.
  • Red blood cells interfere with many diagnostic assays. For example, in the detection of analytes, red blood cells may inhibit the binding between the paired components of specific structures; red blood cells have enzymatic activity, which may interfere with the detection signal.
  • ZL03217482.9 discloses a technical solution for adding an erythrocyte filtration membrane between a colloidal gold conjugate of a colloidal gold test strip and a nitrocellulose membrane in an attempt to overcome the above-mentioned interference effect of red blood cells.
  • This technical measure is not overcome from the "source” component of the strip (sucking pad 4), but the red blood cell filter membrane is introduced between the marking pad 6 of the colloidal gold test strip and the analytical membrane 8. (ie, "between the colloidal gold conjugate and the nitrocellulose membrane" of the test strip of the patent), this "improved” scheme for filtering red blood cells from the "downstream” component of the strip does not effectively overcome the red cell pair marker pad. Interference with the binding reaction between the 6 coatings (gold standard-antigen/antibody) and the target analyte from the aspirating pad 4!
  • the use of the standard curve of the test substance to quantify the concentration of the test substance is the main method for quantifying the concentration of the sample in the current detection field.
  • the standard curve is technically strong and the process is complicated, and it is inseparable from professional technicians.
  • Recently developed storage media in the information field such as RFID tags (also known as radio frequency identification tags), IC chips, magnetic codes, barcodes, etc., for information access identification is now widely used in computers, communications, electronics, commerce, transportation Control management and other fields.
  • RFID tags small size, large storage capacity, identification without manual intervention, is an ideal means of information access identification, but few people use it in biomedical testing.
  • the present invention provides a test information capable of filtering a sample by itself (the sample can be filtered when the whole blood is filtered), and using the self-contained storage medium 14 to store a standard curve or coefficient parameter of the test object. (The operator does not need to make the standard curve of the test object when testing the sample.)
  • the test strip for single-component or multi-component quantitative detection of the sample can be quickly realized.
  • the invention is used for sample detection, and has the characteristics of simple and rapid, high sensitivity and objective result.
  • the test strip card of the present invention comprises a cartridge 3 and a test strip 2.
  • the test strip 2 includes a sample pad 4, a filter pad 5, a marker pad 6, an analysis film 8, and an absorbent pad 9 which are fixed to the substrate 1 in this order.
  • the analysis membrane 8 has a detection strip 7.
  • the detection tape 7 is one or more.
  • the upper casing of the cartridge 3 is provided with a detection window 12 at a position corresponding to the test strip analysis film 8.
  • a storage medium 14 is mounted on the cartridge 3.
  • One end of the cartridge 3 and the upper opening of the cartridge end form a test strip slot 11 to facilitate the insertion of the test strip 2 into the cartridge 3.
  • the test strip 2 is a separate insertion structure of the cartridge 3. At the time of sample detection, the test strip 2 inserted into the cartridge 3 has one end of the sample pad 4 interfering with the open end of the cartridge 3 to be immersed to suck the sample.
  • the filter mat 5 of the test strip 2 includes a blood filter membrane.
  • the marker pad 6 of the test strip 2 is coated with a single specific molecule associated with a target analyte detected by a single marker, or a specific one associated with each target analyte coated with a marker corresponding to a different marker. a mixture of molecules.
  • the test strip 7 of the test strip 2 is coated with another specific molecule associated with detection of a certain target analyte, or a mixture of another specific molecule coated with detection of each target analyte.
  • the storage medium 14 on the cartridge 3 includes, but is not limited to, an RFID tag, an IC chip, a magnetic code, or a barcode.
  • the storage medium 14 stores the standard curve or coefficient parameter of the sample for the quantitative sample concentration of the same batch test strip 2, the test strip batch number, the test strip validity period, the storage medium password, the clinical index reference value, and the test strip manufacturer information. Etc., and can read information such as the identity information of the object to be tested, the information of the detector, the name of the sample, the sample number, the date of detection, and the test result.
  • the standard curve of the sample stored in the storage medium 14 can be selected in various forms including, but not limited to, a corresponding relationship between the concentration of the sample standard series and the A detection zone .
  • the A detection zone is defined as the detection zone signal strength measured by the concentration of the sample standard series.
  • a test strip reaction end indicating label 10 can be overlapped.
  • the test strip reaction end point of the cartridge 3 indicates that the label 10 has a viewing window 13 corresponding thereto.
  • the strip reaction end point indicator 10 includes, but is not limited to, a pH test strip having a discoloration range of 5-9.
  • the marking pad 6 of the test strip 2 of the present invention is a glass fiber membrane;
  • the analysis membrane 8 of the test strip 2 is a nitrocellulose membrane, a nylon membrane, or a mixed membrane of nitrocellulose/cellulose acetate;
  • the backing 1 is a polyester or plastic plate;
  • the cartridge 3 is made of polyester, plastic, hard paper material, or other material.
  • the test strip 2 of the present invention is any test strip, including but not limited to a quantum dot label test strip, a colloidal gold label test strip, a colloidal selenium label strip, an upconversion phosphor label strip, and a nano rare earth fluorescent complex labeling test. Strips, nano-magnetic particle labeling strips, time-resolved chromatographic strips, chemiluminescent strips.
  • test strip 2 of the present invention is a disposable product, and the storage medium 14 on the cartridge 3 and the cartridge 3 is a used product matched with the same batch test strip 2.
  • the test strip of the present invention When the test strip of the present invention is used for sample detection, the test strip 2 is currently inserted into the cartridge 3, and the liquid sample is immersed in the interference projection end of the test strip 2 inserted into the cartridge 3. The liquid sample is slowly pulled toward the rear end of the test strip 2 by capillary siphoning of the microporous filter at the pulling of the absorbent pad 9 at the other end of the test strip 2 (the filter pad 5 is first passed to the marking pad 6 with the marking pad). The labeling conjugate of 6 is combined with the reaction and continues to migrate to the detection zone 7 of the analysis membrane 8 to complete the strip reaction.
  • the liquid sample is filtered in the filter pad area 5 after the "source” component (absorption pad 4) of the test strip to avoid interference of the sample in the sample to the test strip immediately after the filter mat 5 Cause interference.
  • the test strip filter pad 5 will prevent the red blood cells in the blood from passing through and only allow the serum to be filtered, and the filter pad 5 serves to separate the serum (red blood cells can not only Prevents the smooth migration of the sample on the test strip 2, and when the sample is tested with a colloidal gold test strip, the red color of the red blood cells will be affected by the red or magenta color displayed by the colloidal gold mark on the test strip.
  • test strip 2 When the test strip 2 is used for qualitative test of the sample, it will affect the observation and judgment of the test result; when the test strip 2 is used for quantitative test of the sample, it will affect the true signal strength of the test object accurately measured by the test instrument. , thereby affecting the quantitative concentration of the sample).
  • the test strip of the present invention collects the test strip in the test strip card by the instrument with the signal detection function (such as the reading instrument, the fluorescence detector, the magnetic signal detector, etc.) after the test strip is tested and the test strip is completed.
  • the detection zone 7 characteristic signal is combined with the standard curve or coefficient parameter of the test object simultaneously read by the instrument from the storage medium 14 to calculate the single component or multi-component concentration of the sample.
  • the sample to be tested of the test strip card of the present invention may be from clinical or non-clinical blood, body fluid, urine, saliva, genital secretions or other liquid samples or viscous samples, wherein the clinical samples include infectious diseases, Samples of hormones, cardiovascular diseases, tumors, cancer, diabetes, autoimmune diseases, etc., non-clinical samples including food testing, environmental pollution testing, pesticide residue testing, biological contamination testing, biological agent testing, veterinary testing, drugs Samples such as detection.
  • the present invention has the following beneficial effects:
  • the sample can be filtered to reduce the interference effect of the components affected by the sample detection.
  • the sample to be tested is a whole blood sample
  • the whole blood can be directly detected.
  • the existing test strip technology detects blood samples, it is necessary to separate the serum by using equipment such as a centrifuge or a centrifuge tube, and then perform detection, and the whole blood sample cannot be detected.
  • a storage medium 14 is mounted on the test strip card of the present invention, and the storage medium 14 stores detection information such as a standard curve or a coefficient parameter of the test object. After the sample to be tested completes the chromatographic reaction on the test strip, the characteristic signal of the test strip 7 of the test strip 2 in the test strip card is collected by an instrument having a signal detecting function and is simultaneously read from the storage medium 14 by the instrument.
  • the sample standard curve or coefficient parameter allows for rapid (in minutes) quantitative sample component concentration. Existing test strip technology does not have this function.
  • Quantum Dots are semiconductor nanocrystals with excellent spectral characteristics and photochemical stability developed in the 1990s. They have high fluorescence emission efficiency, wide excitation line range, narrow emission line range, and fluorescence. It has a long life, large Stokes displacement, similar particle size to biomolecules, and multi-functionality after surface modification. Quantum dots of different particle sizes, types and structures can produce sustained fluorescence peak spectra with different characteristic wavelengths, and the characteristic wavelength fluorescence peak spectra produced by the quantum dot mixture do not overlap.
  • the corresponding reaction molecules of the test object are respectively labeled by using different quantum dots, and the mixture is coated on the test strip of the test strip of the present invention to react with the sample to be tested, and the specific fluorescent signal of the test strip can be measured within a few minutes.
  • the existing test strip technology can only qualitatively detect samples, and can not achieve quantitative detection of samples, and can not achieve quantitative detection of multi-component samples.
  • the reagents and materials in the test strip are not involved in activity inactivation and can be stored at room temperature for a long time.
  • Figure 1 is a top plan view of a preferred embodiment of the test strip card of the present invention (showing a test line in the inner test strip)
  • Figure 2 is a side view showing the structure of the test strip in the cartridge of the first preferred embodiment of the strip card of the present invention (the test strip adopts a test line)
  • Figure 3 is a top plan view of a preferred embodiment of the test strip card of the present invention (showing a plurality of test lines in the test strip)
  • Figure 4 is a side view showing the structure of the test strip in the cartridge of the first preferred embodiment of the test strip card of the present invention (the test strip adopts a plurality of test lines)
  • Figure 5 is a top plan view of another preferred embodiment of the test strip of the present invention (showing a test line in the test strip)
  • Figure 6 is a side view showing the structure of the test strip in another embodiment of the test strip card of the present invention (the test strip adopts a test line)
  • Figure 7 is a top plan view of another preferred embodiment of the test strip card of the present invention (showing a plurality of test lines in the test strip)
  • Figure 8 is a side elevational view of the test strip of the cartridge in another preferred embodiment of the strip card of the present invention (the test strip uses a plurality of test lines).
  • test strip 1, underlay, 2, test strip, 3, card box, 4, suction pad, 5, filter pad, 6, marker pad, 7, test tape, 8, analysis film, 9. Absorbent pad, 10, test strip reaction end indication label, 11, test strip slot, 12, detection window, 13, observation window, 14, storage medium.
  • Embodiment 1 is one of the preferred embodiments of the test strip card of the present invention.
  • the test strip card of the preferred embodiment includes a cartridge 3 and a test strip 2.
  • the test strip 2 includes a sample pad 4, a filter pad 5, a marker pad 6, an analysis film 8, and an absorbent pad 9 which are sequentially attached to the substrate 1 by overlapping.
  • the analysis membrane 8 has a detection strip 7.
  • the upper casing of the cartridge 3 is provided with a detection window 12 at a position corresponding to the test strip analysis film 8.
  • a storage medium 14 is mounted on the cartridge 3.
  • One end of the cartridge 3 and the upper opening of the cartridge end form a test strip slot 11 to facilitate the insertion of the test strip 2 into the cartridge 3.
  • the test strip 2 is a separate insertion structure of the cartridge 3. At the time of sample detection, the test strip 2 inserted into the cartridge 3 has one end of the sample pad 4 interfering with the open end of the cartridge 3 to be immersed to suck the sample.
  • the filter mat 5 of the test strip 2 includes a blood filter membrane.
  • the marker pad 6 of the test strip 2 is coated with a single specific molecule associated with a target analyte detected by a single marker, or a specific one associated with each target analyte coated with a marker corresponding to a different marker. a mixture of molecules.
  • the test strip 7 of the test strip 2 may be one (shown in Figures 1 and 2) or a plurality of strips (shown in Figures 3 and 4).
  • the test strip 7 is coated with another specific molecule associated with detection of a target analyte, or a mixture of another specific molecule coated with detection of each target analyte.
  • the storage medium 14 on the cartridge 3 includes, but is not limited to, an RFID tag, an IC chip, a magnetic code, or a barcode.
  • the storage medium 14 stores the standard curve or coefficient parameter of the sample for the quantitative sample concentration of the same batch test strip 2, the test strip batch number, the test strip validity period, the storage medium password, the clinical index reference value, and the test strip manufacturer information. Etc., and can read information such as the identity information of the object to be tested, the information of the detector, the name of the sample, the sample number, the date of detection, and the test result.
  • the standard curve of the sample stored in the storage medium 14 can be selected in various forms including, but not limited to, a corresponding relationship between the concentration of the sample standard series and the A detection zone .
  • the A detection zone is defined as the detection zone signal strength measured by the concentration of the sample standard series.
  • the marking pad 6 of the test strip 2 is a glass fiber membrane; the analysis membrane 8 of the test strip 2 is a nitrocellulose membrane, a nylon membrane, or a nitrocellulose/cellulose acetate mixed membrane; the bottom of the test strip 2
  • the liner 1 is a polyester or plastic plate; the cartridge 3 is made of polyester, plastic, hard paper material, or other material.
  • the test strip 2 is any test strip, which includes, but is not limited to, a quantum dot label test strip, a colloidal gold label test strip, a colloidal selenium label strip, an upconversion phosphor label strip, a nano rare earth fluorescent complex label strip, Nano magnetic particle labeling strip, time-resolved chromatographic strip, chemiluminescent strip.
  • the test strip 2 is a disposable product.
  • the storage medium 14 on the cartridge 3 and the cartridge 3 is a used product matched with the same batch of test strips 2.
  • the test strip 2 When the test strip card is used for sample detection, the test strip 2 is currently inserted into the cartridge 3, and the liquid sample is immersed in the interference projection end of the test strip 2 inserted into the cartridge 3. After the liquid sample is subjected to the test strip reaction on the test strip 2, the characteristic signal of the test strip 7 of the test strip 2 is collected by an instrument having a signal detecting function (such as a reading instrument, a fluorescence detector, a magnetic signal detector, etc.) and combined with the instrument. The sample one-component or multi-component concentration is calculated from the test sample standard curve or coefficient parameter simultaneously read from the test strip storage medium 14.
  • a signal detecting function such as a reading instrument, a fluorescence detector, a magnetic signal detector, etc.
  • the sample to be tested of the test strip card may be from clinical or non-clinical blood, body fluid, urine, saliva, genital secretions or other liquid samples or viscous samples, wherein the clinical samples include infectious diseases, hormones, Samples of cardiovascular disease, cancer, cancer, diabetes, autoimmune diseases, etc., non-clinical samples including food testing, environmental pollution testing, pesticide residue testing, biological contamination testing, biological agent testing, veterinary testing, drug testing, etc.
  • Embodiment 2 is another preferred embodiment of the test strip card of the present invention.
  • the strip card of the preferred embodiment includes a cartridge 3 and a strip 2.
  • the test strip 2 includes a sample pad 4, a filter pad 4, a marker pad 6, an analysis film 8, an absorbent pad 9, and a test strip reaction end indicating label 10 which are sequentially attached to the substrate 1.
  • the analysis membrane 8 has a detection strip 7.
  • the upper casing of the cartridge 3 is provided with a detection window 12 at a position corresponding to the test strip analysis film 8.
  • the upper casing of the cartridge 3 is provided with an observation window 13 at a position corresponding to the test strip end point indicating label 10.
  • a storage medium 14 is mounted on the cartridge 3.
  • One end of the cartridge 3 and the upper opening of the cartridge end form a test strip slot 11 to facilitate the insertion of the test strip 2 into the cartridge 3.
  • the test strip 2 is a separate insertion structure of the cartridge 3. At the time of sample detection, the test strip 2 inserted into the cartridge 3 has one end of the sample pad 4 interfering with the open end of the cartridge 3 to be immersed to suck
  • the filter mat 5 of the test strip 2 includes a blood filter membrane.
  • the marker pad 6 of the test strip 2 is coated with a single specific molecule associated with a target analyte detected by a single marker, or a specific one associated with each target analyte coated with a marker corresponding to a different marker. a mixture of molecules.
  • the test strip 7 of the test strip 2 may be one (shown in FIGS. 5 and 6) or a plurality of strips (shown in FIGS. 7 and 8).
  • the test strip 7 is coated with another specific molecule associated with detection of a target analyte, or a mixture of another specific molecule coated with detection of each target analyte.
  • the strip reaction end point indicator 10 includes, but is not limited to, a pH test strip having a discoloration range of 5-9.
  • the test strip reaction end point on the test strip indicates that the label 10 can be colored according to the pH condition of the test strip reaction, to indicate whether the test strip reactant has sufficiently oozing through the test strip 7, indicating whether the strip reaction is sufficient, Whether the test result is valid.
  • the storage medium 14 on the cartridge 3 includes, but is not limited to, an RFID tag, an IC chip, a magnetic code, or a barcode.
  • the storage medium 14 stores the standard curve or coefficient parameter of the sample for the quantitative sample concentration of the same batch test strip 2, the test strip batch number, the test strip validity period, the storage medium password, the clinical index reference value, and the test strip manufacturer information. Etc., and can read information such as the identity information of the object to be tested, the information of the detector, the name of the sample, the sample number, the date of detection, and the test result.
  • the standard curve of the sample stored in the storage medium 14 can be selected in various forms including, but not limited to, a corresponding relationship between the concentration of the sample standard series and the A detection zone .
  • the A detection zone is defined as the detection zone signal strength measured by the concentration of the sample standard series.
  • the marking pad 6 of the test strip 2 is a glass fiber membrane; the analysis membrane 8 of the test strip 2 is a nitrocellulose membrane, a nylon membrane, or a nitrocellulose/cellulose acetate mixed membrane; the bottom of the test strip 2
  • the liner 1 is a polyester or plastic plate; the cartridge 3 is made of polyester, plastic, hard paper material, or other material.
  • the test strip 2 is any test strip, which includes, but is not limited to, a quantum dot label test strip, a colloidal gold label test strip, a colloidal selenium label strip, an upconversion phosphor label strip, a nano rare earth fluorescent complex label strip, Nano magnetic particle labeling strip, time-resolved chromatographic strip, chemiluminescent strip.
  • the test strip 2 is a disposable product.
  • the storage medium 14 on the cartridge 3 and the cartridge 3 is a used product matched with the same batch of test strips 2.
  • test strip 2 When the test strip card is used for sample detection, the test strip 2 is currently inserted into the cartridge 3, and the liquid sample is immersed in the interference projection end of the test strip 2 inserted into the cartridge 3.
  • the test strip reaction end indication label 10 indicates that the sample is subjected to the test strip reaction on the test strip 2, Collecting the characteristic signal of the detection zone 7 of the test strip 2 in the test strip card by an instrument having a signal detection function (such as a reading instrument, a fluorescence detector, a magnetic signal detector, etc.) and combining the instrument from the test strip card storage medium 14
  • the sample standard curve or coefficient parameter is read to calculate the single component or multi-component concentration of the sample.
  • the sample to be tested of the test strip card may be from clinical or non-clinical blood, body fluid, urine, saliva, genital secretions or other liquid samples or viscous samples, wherein the clinical samples include infectious diseases, hormones, Samples of cardiovascular disease, cancer, cancer, diabetes, autoimmune diseases, etc., non-clinical samples including food testing, environmental pollution testing, pesticide residue testing, biological contamination testing, biological agent testing, veterinary testing, drug testing, etc.
  • test strip card of the present invention may have other improvements.
  • the storage medium 14 storing the detection information such as the standard curve of the test object may be mounted on the cartridge 3 of the test strip card.
  • the test strip in the cartridge 3 can also be expanded and replaced with a biochip (including an antigen-antibody chip, a protein chip, a nucleic acid chip, a microfluidic chip), etc. Wait. Therefore, any other technical solutions formed by any equivalent replacement or equivalent transformation of the test strip of the present invention fall within the scope of protection of the present invention.

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Abstract

La présente invention porte sur une carte à bâtonnet diagnostique pouvant auto-filtrer un échantillon et ayant un support d'informations (14) pour mémoriser une courbe standard ou des informations de paramètre de coefficient d'un objet à analyser ; la carte à bâtonnet diagnostique comprend un boîtier de carte (3)et un bâtonnet diagnostique (2) ; une fente d'insertion (11) de bâtonnet diagnostique est formée dans l'ouverture au niveau d'une extrémité du boîtier de carte (3) ; le bâtonnet diagnostique (2) est une structure d'insertion détachée du boîtier de carte (3) ; le support d'informations (14) est installé sur le boîtier de carte (3) ; lors de la réalisation de l'essai d'échantillon, une extrémité d'un tampon d'absorption d'échantillon (4) du bâtonnet diagnostique (2) inséré dans le boîtier de carte (3) s'étend hors de l'extrémité d'ouverture du boîtier de carte (3) pour plonger dans et absorber l'échantillon, et un instrument ayant une fonction de détection de signal collecte le signal caractéristique d'une bande de détection (7) du bâtonnet diagnostique (2), et combine la courbe standard ou le paramètre de coefficient de l'objet à analyser simultanément lu depuis le support d'informations (14) de manière à calculer et obtenir la concentration de composant unique ou multi-composant de l'échantillon. La présente invention est commode et rapide, et flexible à utiliser, et est hautement sensible et produit des résultats objectifs.
PCT/CN2014/090851 2013-11-15 2014-11-11 Carte à bâtonnet diagnostique WO2015070748A1 (fr)

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CN201310564945.1A CN103675269A (zh) 2013-11-15 2013-11-15 一种试条卡
CN201310564945.1 2013-11-15

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CN103630682A (zh) * 2013-11-12 2014-03-12 成都领御生物技术有限公司 一种量子点标记的试条卡
CN103630685A (zh) * 2013-11-13 2014-03-12 成都领御生物技术有限公司 一种试条卡
CN103645311A (zh) * 2013-11-13 2014-03-19 成都领御生物技术有限公司 一种试条卡
CN103675269A (zh) * 2013-11-15 2014-03-26 成都领御生物技术有限公司 一种试条卡
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