WO2015070746A1 - Carte de bandelette d'essai marquée par des points quantiques - Google Patents

Carte de bandelette d'essai marquée par des points quantiques Download PDF

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Publication number
WO2015070746A1
WO2015070746A1 PCT/CN2014/090849 CN2014090849W WO2015070746A1 WO 2015070746 A1 WO2015070746 A1 WO 2015070746A1 CN 2014090849 W CN2014090849 W CN 2014090849W WO 2015070746 A1 WO2015070746 A1 WO 2015070746A1
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Prior art keywords
test strip
quantum dot
sample
cds
zns
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PCT/CN2014/090849
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English (en)
Chinese (zh)
Inventor
马义才
顾敏
马灵
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成都领御生物技术有限公司
马义才
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Publication of WO2015070746A1 publication Critical patent/WO2015070746A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases

Definitions

  • the invention belongs to the field of in-vitro diagnosis, and particularly relates to a quantum dot mark capable of rapidly quantifying a single component or a multi-component concentration of a sample by combining an instrument with a test standard curve or a coefficient parameter and the like, and an instrument having a signal detection function.
  • Test strip card
  • GICA Gold-Immunochromatography Assay
  • nano quantum dots have unique and excellent optical properties: high fluorescence efficiency, wide excitation line range, single-element excitation, multiple emission, narrow emission spectrum and symmetry, and slow photobleaching speed.
  • the fluorescence lifetime is long, the particle size is similar to that of biomolecules, and the surface modification can be multi-functionalized.
  • the characteristic wavelength fluorescence spectra produced by the mixture of quantum dots of different particle sizes and kinds do not overlap, which is very suitable for sample multi-component analysis. Sweeny et al.
  • Goldman's group used multicolor quantum dots to simultaneously detect enterotoxin B and 2,4,6-trinitrotoluene produced by staphylococci and four toxins of cholera toxin, ricin, Shiga toxin and staphylococcal toxin.
  • quantum dots are used as fluorescent labels in microfluidic protein chips, and their fluorescent pleochroism can simultaneously detect tumor markers carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) with detection limits as low as 250fmol. /L, 4 orders of magnitude higher than detection with organic fluorescent dyes.
  • CEA carcinoembryonic antigen
  • AFP alpha-fetoprotein
  • VEGF A vascular endothelial growth factor A
  • angiopoietin angiopoietin
  • quantum dots of different emission wavelengths respectively, and causing aggregation of quantum dots by antigen-antibody specific binding reaction, using flow cytometry
  • the characteristic fluorescent signal was quantitatively detected to obtain the concentration of the corresponding antigen, and the detection limit was 1 pmol/L, and simultaneous detection of various antigens was achieved.
  • quantum dot coding to perform multi-component analysis. This technology is mainly encoded by the richness of quantum dot fluorescence color and fluorescence intensity. It can simultaneously measure more components and achieve simultaneous analysis of massive information in protein analysis, DNA analysis and drug screening. .
  • n kinds of intensity m colors can generate n m -1 kinds of codes, and the detection and detection of n m -1 kinds of targets can be realized by decoding technology.
  • the calculation only 50,000 to 40,000 identifiable nanoparticle-encoded microspheres can be obtained by combining 5 to 6 colors with 6 quantum dots of different luminescence intensity. If combined with 10 quantum-intensity quantum dot nanoparticles, one million identifiable nanoparticle-encoded microspheres can be obtained. These microspheres can theoretically encode 1 million different DNA or proteins. Simultaneous detection of 1 million different DNAs or proteins in a sample! Nie et al.
  • Wilson and Cooper used this technology to achieve three serum proteins (chicken ovalbumin OVA, bovine serum albumin BSA, human serum albumin HAS) and four immunoglobulin G (human IgG, rabbit IgG, murine IgG, sheep). Simultaneous detection of IgG).
  • Chinese Patent Application No. 200610024086.7 discloses a method for detecting a quantum dot-labeled rapid immunochromatographic test strip.
  • the patent enables multi-component simultaneous detection, it is a method that is claimed, and no request for protection of a quantum dot-labeled rapid immunochromatographic test strip product has been proposed.
  • this method has some shortcomings: (1) To achieve multi-component detection of samples, it is necessary to simultaneously set a plurality of corresponding detection lines on the analysis membrane, that is, each detection line is only used to detect one index in the sample, and the detection efficiency is low.
  • the detector wants to simultaneously detect a large number of components, flux components, and even large components in the sample, it is impossible for a small test strip to achieve the detection target by setting such a large number of corresponding detection lines.
  • Quantifying the concentration of the test substance using the standard curve of the test subject standard is the main method for quantifying the concentration of the sample in the field of detection.
  • Recently developed storage media in the information field such as RFID tags (also known as radio frequency identification tags), IC chips, magnetic codes, barcodes, etc., for information access identification is now widely used in computers, communications, electronics, commerce, transportation Control management and other fields, especially RFID tags, small size, large storage information capacity, identification without manual intervention, is an ideal means of information access identification, but few people currently use it for biomedical testing.
  • the present invention is directed to the deficiencies and shortcomings of the prior art, and provides a quantum dot marking strip card on which a storage medium 13 is mounted, the storage medium 13 storing the same batch of quantum dot marking strips 2 for quantitative detection of the sample.
  • Information for testing such as standard curve or coefficient parameters for concentration.
  • the characteristic signal of the test strip 2 in the test strip 2 is collected by the instrument having the signal detecting function, and the detected object is simultaneously read from the storage medium 13 in combination with the apparatus.
  • the standard curve or coefficient parameters are calculated to obtain the one-component or multi-component concentration of the sample.
  • the invention is characterized in that the sample concentration, in particular the multi-component concentration of the sample, is simple, rapid, sensitive, objective, and flexible.
  • the quantum dot-labeled test strip card of the present invention comprises a cartridge 3 and a quantum dot marking strip 2.
  • the quantum dot marking test strip 2 includes a sample pad 4, a marking pad 5, an analysis film 7, and an absorbent pad 8 which are sequentially attached to the substrate 1 by overlapping.
  • the analysis membrane 7 has a detection strip 6.
  • One end of the cartridge 3 and the upper opening of the cartridge end form a test strip slot 12 to facilitate insertion of the quantum dot marking strip 2 into the cartridge 3.
  • the test strip 2 is a separate insertion structure of the cartridge 3. At the time of sample detection, the test strip 2 inserted into the cartridge 3 has one end of the sample pad 4 interfering with the open end of the cartridge 3 to be immersed to suck the sample.
  • the upper casing of the cartridge 3 is provided with a detection window 11 at a position corresponding to the test strip analysis film 7.
  • a storage medium 13 is mounted on the cartridge 3.
  • the marking pad 5 of the quantum dot marking strip 2 is coated with a single specific molecule related to a target detection of a single quantum dot label, or: a target analyte coated with a label corresponding to a single quantum dot A mixture of related specific molecules is detected.
  • the single quantum dots coated by the marking pad 5 include ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag 2 S, CdS/PbS, CdS/Cd(OH) 2 , CdS/HgS, CdS/HgS/CdS, ZnS/CdS, ZnS/CdS/ZnS, ZnS/HgS/ZnS/CdS, CdSe/CdS, CdSe/ZnS, CdSe/ZnSe, CdSe/CuSe, CdSe/HgTe, CdSe/HgSe, CdSe/HgSe/CdSe, CdTe/HgS, C
  • the detection band 6 of the quantum dot marking strip 2 may be one piece or a plurality of pieces.
  • the test strip 6 is coated with another specific molecule associated with detection of a target analyte, or a mixture of another specific molecule coated with detection of each target analyte.
  • the storage medium 13 includes, but is not limited to, an RFID tag, an IC chip, a magnetic code, or a barcode.
  • the storage medium 13 stores the standard curve or coefficient parameter of the sample for quantitative sample concentration of the same batch of quantum dot labeling strip 2, the test strip batch number, the strip validity period, the storage medium password, the clinical index reference value, and the test strip. Manufacturer information, etc., and can read information such as the identity information of the object to be tested, the information of the detector, the name of the sample, the sample number, the date of detection, and the test result.
  • the standard curve of the test object stored in the storage medium 13 can be selected in various forms, including but not limited to the corresponding relationship between the concentration of the sample standard series and the OD detection zone .
  • the OD detection zone is defined as the detection band optical density value measured by the concentration of the sample standard series.
  • a test strip reaction end point indicating label 9 can be overlapped.
  • the test strip reaction end point of the cartridge 3 indicates that the label 9 has a viewing window 11 corresponding thereto.
  • the strip reaction end point indicating label 9 includes a pH test strip having a discoloration range of 5-9.
  • the marking pad 5 of the quantum dot marking strip 2 of the present invention is a glass fiber membrane; the analysis membrane 7 of the quantum dot marking strip 2 is a nitrocellulose membrane, a nylon membrane, or a nitrocellulose/cellulose acetate mixed membrane.
  • the underlayer 1 of the quantum dot marking strip 2 is a polyester or plastic plate; the cartridge 3 is made of polyester, plastic, hard paper material, or other material.
  • the quantum dot marking test strip 2 of the present invention is a disposable product, and the storage medium 13 on the cartridge 3 and the cartridge 3 is a used product matched with the same batch of quantum dot marking test strips 2.
  • the quantum dot marking strip card of the present invention When the quantum dot marking strip card of the present invention is used for sample detection, the quantum dot marking strip 2 is currently inserted into the cartridge 3, and the interference protruding end of the strip 2 inserted into the cartridge 3 is immersed in the liquid. sample. After the liquid sample is subjected to the test strip reaction on the test strip 2, the characteristic signal of the strip 2 is collected by the instrument having the signal detecting function (such as a reading instrument, a fluorescence detector, etc.) and combined with the instrument to store the strip from the strip. The storage medium 13 simultaneously reads the sample standard curve or coefficient parameter to calculate the single component or multi-component concentration of the sample.
  • the instrument having the signal detecting function such as a reading instrument, a fluorescence detector, etc.
  • the sample to be tested of the quantum dot-labeled test strip card of the present invention may be from clinical or non-clinical blood, body fluid, urine, saliva, genital tract secretion or other liquid sample or viscous sample, wherein the clinical sample includes Samples of infectious diseases, hormones, cardiovascular diseases, tumors, cancer, diabetes, autoimmune diseases, etc., non-clinical samples including food testing, environmental pollution testing, pesticide residue testing, biological contamination testing, biological agent testing, veterinary medicine Samples such as testing, drug testing, etc.
  • the present invention has the following beneficial effects:
  • the test strip card combined with the instrument with signal detection function can realize synchronous multi-component rapid quantitative detection (including qualitative detection).
  • Quantum dots of different particle sizes, types and structures can produce continuous fluorescence with different characteristic wavelengths, and the characteristic wavelength fluorescence peak spectra generated by the quantum dot mixture do not overlap.
  • the present invention uses different quantum dots to respectively mark the corresponding reaction molecules of the analyte, and mixes the same. The coating is reacted with the sample to be tested on the test strip 2 of the test strip card, and the characteristic fluorescent signal of the test strip 2 is measured, thereby enabling rapid quantification of the multi-component concentration of the sample.
  • the time for testing the sample is short, and the result can be obtained in a few minutes.
  • the quantitative sample concentration of the present invention requires the operator to prepare a standard curve of the test object, and the test information such as the standard curve or the coefficient parameter of the test object is stored on the storage medium 13.
  • the operator only needs to put the test strip to which the sample is applied to be completed after the test strip reaction, and then put the instrument with the signal detection function, and the instrument collects the characteristic fluorescent signal of the strip 2 of the test strip 2, and combines the
  • the standard test curve or coefficient parameter of the corresponding test object read from the storage medium 13 can quantitatively obtain the sample test result in a few minutes.
  • the amount of sample is small, and a few microliters to several tens of microliters can meet the detection needs.
  • the reagents and materials in the test strip are not involved in activity inactivation and can be stored at room temperature for a long time.
  • Figure 1 is a top plan view of a preferred embodiment of the quantum dot-labeled test strip card of the present invention (in which a test strip is used as a test line)
  • Figure 2 The preferred embodiment of the quantum dot-labeled test strip card of the present invention is a side view of the inserted test strip (the test strip uses a test line)
  • Figure 3 is a top plan view of a preferred embodiment of the quantum dot-labeled test strip card of the present invention (in which a plurality of test lines are used for inserting test strips)
  • Figure 4 is a side view structural view of a preferred embodiment of the quantum dot-labeled test strip card of the present invention, which is a matching test strip (the test strip uses a plurality of test lines)
  • Figure 5 is a top plan view of another preferred embodiment of the quantum dot-labeled test strip card of the present invention (in which a plug-in test strip adopts a test line)
  • Figure 6 is a side view structural view of a plug-in test strip of another preferred embodiment of the quantum dot-labeled test strip card of the present invention (the test strip adopts a test line)
  • Figure 7 is a top plan view of another preferred embodiment of the quantum dot-labeled test strip card of the present invention (in which a plurality of test lines are used for inserting test strips)
  • Figure 8 is a side view structural view of a plug-in test strip of another preferred embodiment of the quantum dot-labeled test strip card of the present invention (the test strip uses a plurality of test lines)
  • Embodiment 1 is one of the preferred embodiments of the quantum dot marking strip card of the present invention.
  • the quantum dot marking strip card of the preferred embodiment comprises a cartridge 3 and a quantum dot marking strip 2.
  • the quantum dot marking test strip 2 includes a sample pad 4, a marking pad 5, an analysis film 7, and an absorbent pad 8 which are sequentially attached to the substrate 1 by overlapping.
  • the analysis membrane 7 has a detection strip 6.
  • One end of the cartridge 3 and the upper opening of the cartridge end form a test strip slot 12 to facilitate insertion of the quantum dot marking strip 2 into the cartridge 3.
  • the test strip 2 is a separate insertion structure of the cartridge 3.
  • the test strip 2 inserted into the cartridge 3 has one end of the sample pad 4 interfering with the open end of the cartridge 3 to be immersed to suck the sample.
  • the upper casing of the cartridge 3 is provided with a detection window 10 at a position corresponding to the test strip analysis film 7.
  • a storage medium 13 is mounted on the cartridge 3.
  • the marking pad 5 of the quantum dot marking strip 2 is coated with a single specific molecule related to a target detection of a single quantum dot label, or: a target analyte coated with a label corresponding to a single quantum dot A mixture of related specific molecules is detected.
  • the single quantum dot of the test strip 2 marking pad 5 may be selected from the group consisting of ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ ZnS, CdS/Ag 2 S, CdS/PbS, CdS/Cd(OH) 2 , CdS/HgS, CdS/HgS/CdS, ZnS/CdS, ZnS/CdS/ZnS, ZnS/HgS/ZnS/CdS, CdSe/ CdS, CdSe/ZnS, CdSe/ZnSe, CdSe/CuSe, CdSe/HgTe, CdSe/HgSe, CdSe/HgSe/CdSe
  • the detection strip 6 of the quantum dot marking strip 2 may be one (shown in FIGS. 1 and 2) or a plurality of strips (shown in FIGS. 3 and 4).
  • the test strip 6 is coated with another specific molecule associated with detection of a target analyte, or a mixture of another specific molecule coated with detection of each target analyte.
  • the storage medium 13 includes, but is not limited to, an RFID tag, an IC chip, a magnetic code, or a barcode.
  • the standard curve or coefficient parameter of the test object, the test strip batch number, the test strip validity period, the storage medium password, the clinical index reference value, and the test strip manufacturer information are stored in the same batch of quantum dot labeling strip 2 quantitative sample concentration. Etc., and can read information such as the identity information of the object to be tested, the information of the detector, the name of the sample, the sample number, the date of detection, and the test result.
  • the standard curve of the test object stored in the storage medium 13 can be selected in various forms, including but not limited to the corresponding relationship between the concentration of the sample standard series and the OD detection zone .
  • the OD detection zone is defined as the detection band optical density value measured by the concentration of the sample standard series.
  • the marking pad 5 of the quantum dot marking strip 2 is a glass fiber membrane;
  • the analysis membrane 7 of the quantum dot marking strip 2 is a nitrocellulose membrane, a nylon membrane, or a nitrocellulose/cellulose acetate mixed membrane;
  • the underlayer 1 of the quantum dot marking strip 2 is a polyester or plastic plate;
  • the cartridge 3 is made of polyester, plastic, hard paper material, or other material.
  • the quantum dot marking strip 2 is a disposable product, and the storage medium 13 on the cartridge 3 and the cartridge 3 is a used product matched with the same batch of quantum dot marking strips 2.
  • the quantum dot marking test strip card When the quantum dot marking test strip card is used for sample detection, the quantum dot marking test strip 2 is currently inserted into the cartridge 3, and the liquid protruding sample is immersed in the interference protruding end of the test strip 2 inserted into the cartridge 3 to suck the liquid sample.
  • the characteristic signal of the strip 2 After the liquid sample is subjected to the test strip reaction on the test strip 2, the characteristic signal of the strip 2 is collected by the instrument having the signal detecting function (such as a reading instrument, a fluorescence detector, etc.) and combined with the instrument to store the strip from the strip.
  • the storage medium 13 simultaneously reads the standard curve or coefficient parameter of the test object and calculates the sample. Single or multi-component concentration.
  • the sample to be tested of the quantum dot-labeled test strip card may be from clinical or non-clinical blood, body fluid, urine, saliva, genital tract secretion or other liquid sample or viscous sample, wherein the clinical sample includes infectious diseases Samples such as hormones, cardiovascular diseases, tumors, cancer, diabetes, autoimmune diseases, etc.
  • infectious diseases Samples such as hormones, cardiovascular diseases, tumors, cancer, diabetes, autoimmune diseases, etc.
  • Non-clinical samples include food testing, environmental pollution testing, pesticide residue testing, biological contamination testing, biological agent testing, veterinary testing, Samples such as drug testing.
  • Embodiment 2 is another preferred embodiment of the quantum dot marking strip card of the present invention.
  • the quantum dot marking strip card of the preferred embodiment comprises a cartridge 3 and a quantum dot marking strip 2.
  • the quantum dot marking strip 2 includes a sample pad 4, a marker pad 5, an analysis film 7, an absorbent pad 8 and a test strip reaction end indicating label 9 which are sequentially attached to the substrate 1.
  • the analysis membrane 7 has a detection strip 6.
  • One end of the cartridge 3 and the upper opening of the cartridge end form a test strip slot 12 to facilitate insertion of the quantum dot marking strip 2 into the cartridge 3.
  • the test strip 2 is a separate insertion structure of the cartridge 3. At the time of sample detection, the test strip 2 inserted into the cartridge 3 has one end of the sample pad 4 interfering with the open end of the cartridge 3 to be immersed to suck the sample.
  • the upper casing of the cartridge 3 is provided with a detection window 10 at a position corresponding to the test strip analysis film 7, and the upper casing of the cartridge 3 is provided with an observation window 11 at a position corresponding to the test strip end point indicating label 9, the cartridge A storage medium 13 is mounted on the third.
  • the marking pad 5 of the quantum dot marking strip 2 is coated with a single specific molecule related to a target detection of a single quantum dot label, or: a target analyte coated with a label corresponding to a single quantum dot A mixture of related specific molecules is detected.
  • the single quantum dot of the strip mark pad 5 may be selected from the group consisting of ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS.
  • the detection strip 6 of the quantum dot marking strip 2 may be one (shown in FIGS. 5 and 6) or a plurality of strips (shown in FIGS. 7 and 8).
  • the test strip 6 is coated with another specific molecule associated with detection of a target analyte, or a mixture of another specific molecule coated with detection of each target analyte.
  • the strip reaction end point indicating label 9 includes a pH test strip having a discoloration range of 5-9.
  • the test strip reaction end point label 9 on the test strip can be colored according to the pH condition of the test strip reaction, to indicate whether the test strip reactant has fully oozing through the test strip 6, indicating whether the test strip reaction is sufficient, Whether the test result is valid.
  • the storage medium 13 includes, but is not limited to, an RFID tag, an IC chip, a magnetic code, or a barcode.
  • the standard curve or coefficient parameter of the test object, the test strip batch number, the test strip validity period, the storage medium password, the clinical index reference value, and the test strip manufacturer information are stored in the same batch of quantum dot labeling strip 2 quantitative sample concentration. Etc., and can read information such as the identity information of the object to be tested, the information of the detector, the name of the sample, the sample number, the date of detection, and the test result.
  • the standard curve of the test object stored in the storage medium 13 can be selected in various forms, including but not limited to the corresponding relationship between the concentration of the sample standard series and the OD detection zone .
  • the OD detection zone is defined as the detection band optical density value measured by the concentration of the sample standard series.
  • the marking pad 5 of the quantum dot marking strip 2 is a glass fiber membrane;
  • the analysis membrane 7 of the quantum dot marking strip 2 is a nitrocellulose membrane, a nylon membrane, or a nitrocellulose/cellulose acetate mixed membrane;
  • the bottom liner 1 of the quantum dot marking strip 2 is a polyester or plastic plate;
  • the cartridge 3 is polyester, plastic, rigid Made of paper materials or other materials.
  • the quantum dot marking strip 2 is a disposable product.
  • the storage medium 13 on the cartridge 3 and the cartridge 3 is used for the same batch of quantum dot marking strips 2.
  • the quantum dot marking test strip card When the quantum dot marking test strip card is used for sample detection, the quantum dot marking test strip 2 is currently inserted into the cartridge 3, and the liquid protruding sample is immersed in the interference protruding end of the test strip 2 inserted into the cartridge 3 to suck the liquid sample.
  • Test strip reaction end point indication label 9 color display indicator After the strip test is completed on the test strip 2, the characteristic signal of the test strip 2 is detected by the instrument having the signal detection function (such as a reading instrument, a fluorescence detector, etc.) The single component or multi-component concentration of the sample is calculated by combining the standard curve or coefficient parameter of the sample simultaneously read from the test strip storage medium 13 by the instrument.
  • the sample to be tested of the quantum dot-labeled test strip card may be from clinical or non-clinical blood, body fluid, urine, saliva, genital tract secretion or other liquid sample or viscous sample, wherein the clinical sample includes infectious diseases Samples such as hormones, cardiovascular diseases, tumors, cancer, diabetes, autoimmune diseases, etc.
  • infectious diseases Samples such as hormones, cardiovascular diseases, tumors, cancer, diabetes, autoimmune diseases, etc.
  • Non-clinical samples include food testing, environmental pollution testing, pesticide residue testing, biological contamination testing, biological agent testing, veterinary testing, Samples such as drug testing.
  • the quantum dot-labeled test strip card of the present invention may have other improvements, such as a storage medium 13 storing detection information such as a test sample standard curve, in addition to the cartridge 3 mounted on the test strip card. Above and outside, it can also be directly mounted on the quantum dot marking strip 2; based on the same principle of the invention, the quantum dot marking strip inserted into the cartridge 3 can also be extended to replace the quantum dot labeled biochip (including antigenic antibodies). Chips, protein chips, nucleic acid chips, microfluidic chips, etc. Therefore, any other technical solution formed by any equivalent replacement or equivalent transformation of the quantum dot-labeled test strip card of the present invention falls within the scope of protection of the present invention.

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Abstract

La présente invention concerne une carte de bandelette d'essai marquée par des points quantiques, comprenant un boîtier de carte (3) et une bandelette d'essai (2) marquée par des points quantiques ; une fente d'insertion de bandelette d'essai (12) est formée dans une ouverture à une extrémité du boîtier de carte (3) ; la bandelette d'essai (2) est une structure d'insertion détachée associée au boîtier de carte (3) ; le boîtier de carte (3) comporte un support de stockage (13) sur lequel sont stockées les informations de courbe étalon d'un objet échantillon à tester ; lors de la mise en œuvre du test d'un échantillon, une extrémité d'un tampon d'absorption d'échantillon (4) de la bandelette d'essai (2) insérée dans le boîtier de carte (3) s'étend hors de l'extrémité d'ouverture du boîtier de carte (3) pour plonger dans l'échantillon et l'absorber, et un instrument ayant une fonction de détection de signal collecte le signal caractéristique d'une bande de détection (6) de la bandelette d'essai (2) et combine la courbe étalon ou un paramètre de coefficient de l'objet à tester lu simultanément sur le support de stockage (13) afin de calculer et d'obtenir la/les concentrations du composant ou des composants contenus dans l'échantillon. La présente invention est commode, rapide, elle a une grande souplesse d'utilisation et est extrêmement sensible, et produit des résultats objectifs.
PCT/CN2014/090849 2013-11-14 2014-11-11 Carte de bandelette d'essai marquée par des points quantiques WO2015070746A1 (fr)

Applications Claiming Priority (2)

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CN103630685A (zh) * 2013-11-13 2014-03-12 成都领御生物技术有限公司 一种试条卡
CN103675267A (zh) * 2013-11-14 2014-03-26 成都领御生物技术有限公司 一种量子点标记的试条卡
CN103645313A (zh) * 2013-11-16 2014-03-19 成都领御生物技术有限公司 一种试条卡
CN105319359B (zh) * 2014-08-18 2017-02-08 董俊 人肺炎链球菌量子点免疫层析检测卡及其制备方法和应用
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