WO2015030423A1 - Micro-organisme ayant une capacité de production d'acide propionique et composition alimentaire le contenant - Google Patents

Micro-organisme ayant une capacité de production d'acide propionique et composition alimentaire le contenant Download PDF

Info

Publication number
WO2015030423A1
WO2015030423A1 PCT/KR2014/007770 KR2014007770W WO2015030423A1 WO 2015030423 A1 WO2015030423 A1 WO 2015030423A1 KR 2014007770 W KR2014007770 W KR 2014007770W WO 2015030423 A1 WO2015030423 A1 WO 2015030423A1
Authority
WO
WIPO (PCT)
Prior art keywords
propionic acid
forage
acid production
feed
products
Prior art date
Application number
PCT/KR2014/007770
Other languages
English (en)
Korean (ko)
Inventor
정승기
조경현
Original Assignee
주식회사 바이오리쏘스
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 주식회사 바이오리쏘스 filed Critical 주식회사 바이오리쏘스
Priority to JP2016538843A priority Critical patent/JP2016533755A/ja
Publication of WO2015030423A1 publication Critical patent/WO2015030423A1/fr

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A23K10/38—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Definitions

  • the present invention relates to a microorganism having a proonic acid producing ability and a forage composition comprising the same.
  • Volatile fatty acids are the most important end products of carbohydrate breakdown in the rumen. Volatile fatty acids are an important source of energy (70%) for ruminants and also affect the protein and fat content of milk.
  • the main volatile fatty acids produced by the metabolism in the rumen are three types of acetic acid, propionic acid and butyric acid, depending on the type of feed fed and the degree of digestion. Only propionic acid among volatile fatty acids in ruminants contributes to glucose synthesis, and quantitatively glucose is a very important single precursor. Propionic acid makes up 18-20% of the total volatile fatty acids and is converted to blood sugar in the liver to provide energy and used for lactose synthesis. Increasing propionic acid increases blood flow in ruminant epithelial cells, stimulates angiogenesis and increases epithelial cells, promotes the growth of ruminants, including cattle, and improves meat quality.
  • Fiber is the most abundant energy source on the planet. However, at present, a large part of the survey fee usage in Korea depends on imports. The high dependence on imports of forages is also a task that must be resolved in order to secure the competitiveness of domestic livestock farmers. After mushroom production, various attempts have been made to utilize resources such as fiber-rich waste media as byproducts.
  • Korean Patent No. 1144473 relates to a method for preparing fermentation fertilizer for livestock using mushroom by-products as a main raw material, and is characterized by fermentation by adding lactic acid bacteria, Bacillus subtilis and yeast bacteria used as probiotics to mushroom waste medium.
  • Korean Patent No. 1138934 relates to a method for producing a pig feed using a waste mushroom medium, characterized in that the fermentation using a complex microbial fermentation agent that does not contain a microorganism having a propioic acid producing ability.
  • the present inventors conducted a study on the feed for promoting the ruminant feed, the fermentation of the fertilizer in the rumen to increase the production of propioic acid and thereby the present invention based on a microorganism having a propionic acid production capacity that can promote the fattening Completed.
  • An object of the present invention is to provide a ruminant microorganism having a propionic acid production capacity.
  • the present invention also aims to provide a forage composition having an excellent fattening promoting effect.
  • One aspect of the invention provides a microorganism having a propionic acid production ability isolated from the rumen.
  • the microorganism is Lactobacillus mucosae KCCM11440P.
  • KCCM11440P in Lactobacillus mucosa has the 16S rRNA nucleotide sequence of SEQ ID NO: 1 and was identified based on this sequence. 1 shows a phylogenetic tree based on 16S rRNA sequences.
  • Propionic acid is the most important product of carbohydrate breakdown in the rumen, which is converted from ruminant liver to glucose to promote ruminant fattening and contribute to meat improvement.
  • KCCM11440P Lactobacillus mucosa isolated from ruminant on the basis of propionic acid production capacity, KCCM11440P was grown in MRS medium, especially in medium added with vitamin B 12 or sodium lactate was confirmed to have high propion production capacity.
  • Another aspect of the invention provides a forage composition comprising Lactobacillus mucosa KCCM11440P.
  • the term "irradiant composition” refers to a feed composition having a high fiber content, low fat, protein, starch and the like, such as grasses, hay, silage, and the like.
  • the fertilizer composition according to one aspect of the present invention includes KCCM11440P in Lactobacillus mucosa having propionic acid producing ability, thereby increasing the production of propionic acid during fermentation of the fertilizer in the rumen, thereby promoting the fattening of ruminants and improving meat quality.
  • propionic acid is the only volatile fatty acid produced in the rumen, it is converted into glucose and contributes to energy metabolism. Thus, promotion of the fat of ruminants including cattle is determined by propionic acid produced in the rumen.
  • the forage composition comprises mushroom waste medium.
  • Mushroom waste medium refers to a medium obtained by using a sawdust, the main raw material is a sawdust, a secondary material is obtained by using a culture medium for the culture of mushrooms.
  • Sawdust in mushroom waste media is used as a carbon source by fibrinolytic microorganisms in the rumen.
  • KCCM11440P in Lactobacillus mucosa can be cultured in mushroom lung medium to increase propionic acid production, thereby promoting the rearing of ruminants.
  • the mushroom waste medium can be used as a feedstock having excellent digestibility since the waste medium obtained after decomposition by the fibrinolytic bacteria of mushrooms is used as a raw material.
  • the forage composition comprises at least one of vitamin B 12 and lactate.
  • Lactobacillus mucosa KCCM11440P significantly increases propionic acid production when supplemented with vitamin B 12 or lactate, such as sodium lactate, in the medium. Therefore, the inclusion of at least one of vitamin B 12 and lactate in the forage composition increases propioic acid production, thereby promoting fattening.
  • the forage composition promotes the fattening of ruminants.
  • Lactobacillus mucosa which contains propioic acid producing ability, contains KCCM11440P, thereby increasing propioic acid production in the rumen, thereby promoting the rearing of ruminants and contributing to improved meat quality.
  • the forage composition may further comprise a probiotic.
  • Antibiotics are inevitable when raising livestock, but probiotics are widely used due to serious problems caused by misuse. Probiotics contribute to the uptake of beneficial microorganisms in the intestines of animals, thereby inhibiting the growth of pathogenic microorganisms, preventing the occurrence of diseases, and increasing the productivity of livestock.
  • beneficial microorganisms are lactic acid bacteria, subtilis bacteria, yeasts and the like. Lactic acid bacteria produce organic acids, lower the pH to inhibit harmful bacteria that are weak to acid, enhance the activity of digestive enzymes, and in particular, reduce the frequency of diarrhea.
  • Bacillus subtilis produces enzymes that break down high-active carbohydrates, proteins, and lipids, thereby enhancing feed digestion and absorption in the intestine, improving feed efficiency, and reducing stress from digestion, making livestock growth easier. In addition, it has a formal effect, strengthening the intestines of livestock and preventing disease.
  • yeast is present in a form that can be easily digested in the digestive tract of the livestock, by producing a natural flavor component such as alcohol, glutamic acid to enhance the palatability of the feed of the livestock.
  • the forage composition may be provided mixed with a general feed.
  • the forage composition may be mixed with a commercially formulated feed in a ratio of 1: 1 and fed to ruminants twice a day.
  • Appropriate mixing ratios, feeding amount and feeding frequency can be easily determined by those skilled in the art in consideration of the age, weight, health status, etc. of the subject ruminant.
  • Another aspect of the present invention provides a method of raising a ruminant, the method comprising feeding a forage comprising KCCM11440P to Lactobacillus mucosa.
  • the forage may include mushroom waste medium as a main component.
  • the forage may further comprise a probiotic.
  • the forage may further comprise one or more of vitamin B 12 and sodium lactate.
  • the feed may be fed by mixing with a conventional blended feed.
  • ruminants may be, but are not limited to, cattle, sheep, goats, and deer.
  • Lactobacillus mucosa KCCM11440P and a forage composition comprising the same according to one embodiment of the present invention increases the propioic acid production in the rumen of ruminants thereby resulting in excellent fattening and meat improvement.
  • 1 is a phylogenetic tree based on Lactobacillus mucosa of the present invention based on 16S rRNA gene of KCCM11440P.
  • Figure 2 shows the dry matter loss of fermented feed, bran, mushroom by-products, and brewing by-products with time of incubation.
  • Bacteria cultured from rumen juice were diluted to 10 ⁇ 3 to 10 ⁇ 9 per ml of medium and inoculated in Hungate roll tubes, followed by incubation for 24 to 48 hours. Cultured single colonies were isolated and incubated at 120 rpm for 24 hours after inoculation in liquid medium (MRS). All cultures were performed anaerobic at 37 ° C. (Lee, et al., Applied Microbiology and Biotechnology, Vol. 58, pp. 663-668, 2002), and all media and buffers used (Bryant and Burkey, Journal of Dairy). Science, Vol. 82, pp. 780-787, 1953) were sterilized at 121 ° C. for 15 minutes and O 2 and N 2 gases were used.
  • PCR reaction was performed using 27F primer (AGAGTTTGATCMTGGCTCAG) and 1492R primer (GGTTACCTTGTTACGACTT) for amplification of the gene encoding 16S rRNA.
  • PCR conditions consisted of 32 cycles of a cycle consisting of an initial denaturation step of 5 min at 94 ° C., 45 sec denaturation at 94 ° C., 45 sec annealing at 65 ° C., and 1 min elongation at 72 ° C., and 10 min at 72 ° C. It was kidney.
  • the amplified ribosomal DNA was analyzed for similarity by ARDRA (Amplified Ribosomal DNA Restriction Analysis) method.
  • ARDRA Analog Ribosomal DNA Restriction Analysis
  • the PCR products and the HaeIII HhaI restriction enzymes (Takara, Japan) at 37 °C for 5 hours, and were separated for 80 minutes the obtained DNA sample as a metaphor 170v through the electrophoresis using a gel with agar. Thereafter, visualization was performed using a Kodak Gel Logic 200 imaging system (Eastman Kodak Company, Rochester, NY, USA), and bands obtained from ARDRA were purified using a QIA quick PCR Purification Kit.
  • the purified 16S rDNA PCR product was identified by SEQ ID NO: 1 by analyzing the nucleotide sequence in Macrogen (Korea).
  • the analyzed sequencing information was combined using SeqMan program (DNA Star, Lasergene software, Madison, WI, USA), and the base sequences were NCBI ( http://www.ncbi.nlm.nih.gov/BLAST ) and EzTaxon Comparison was made using the BLAST program at GeneBank ( http://147.47.212.35:8080/index.jsp ).
  • Approximate phylogenetic classification was determined using CLUSTRAL W version 1.6 to compare sequences with the nearest species.
  • the phylogenetic tree was prepared as disclosed in Kimura (1980) using a neighbor-joining (NJ) method with pair-wise gap removal. Finally, the two-parameter NJ method in the PHYLIP package was used, and the bootstrap analysis method was used to collect 1000 times more data to evaluate the stability of the tree. Only bootstrap values of 50% or more are shown. 1 shows the phylogenetic tree created.
  • the isolated strain showed 96% similarity to S32 (T) in Lactobacillus mucosa. Based on this, the isolated strain was named BR-PP to Lactobacillus mucosa, and it was deposited with the Korea Microorganism Conservation Center on July 26, 2013 and received an accession number of KCCM11440P.
  • Propionibacterium axidipropionis showed high propionic acid production after 144 hours. In addition, Propionibacterium axidipropionis showed conditional anaerobic unlike other propionic acid producing strains that are either complete or essential anaerobic. High propionic acid production and conditional anaerobic propionibacterium axidipropionis were selected as the propionic acid producing standard microorganisms and used as controls.
  • the propionic acid production capacity of Lactobacillus mutosa isolated and identified in Example 1 was evaluated.
  • the agar was cultured in anaerobic state for 48 hours in MRS medium.
  • the medium was placed in a Hungate tube and autoclaved at 121 ° C. for 15 minutes after filling with pH 6.5, O 2 -free 20% CO 2 -80% N 2 gas.
  • Biotin 0.5 mg / L
  • vitamin B 12 50 ⁇ g / L
  • glycerol (2%) was added to agar of MRS medium to confirm the effect on propionic acid production capacity, respectively.
  • the control for the evaluation of propionic acid production capacity was propionibacterium acidipropioni selected in Example 1.
  • Samples were taken at 24, 48 and 72 hours of culture to determine the production of volatile fatty acids, including OD, pH and propionic acid.
  • the pH was not measured directly in the incubator but stabilized at the same temperature as room temperature, and then measured using an M503P meter (wrks, Medififield, MA, USA).
  • the amount of volatile fatty acid produced was analyzed after centrifugation of the culture for each time period at 1000 ⁇ g 4 ° C. for 10 minutes, and then the supernatant was collected and purified with a 0.2 ⁇ m micro filter.
  • HPLC analysis was carried out at 35 ° C. using HPLC (Agilent technolgies 1200 series) equipped with a METACARB87H (Varian, Germany) column, and the UV wavelengths of the detectors were 210 nm and 220 nm.
  • the mobile phase solvent was 0.0085 NH 2 SO 4 and the flow rate was 0.6 ml / min.
  • Lactobacillus mucosa KCCM11440P produces the highest amount of propionic acid when cultured in MRS medium supplemented with vitamin B 12 or sodium lactate.
  • Rumen and buffer were used to evaluate the fermentation effect in the rumen in In Vitro .
  • Gastric fluid was collected for in vitro testing using 600 ⁇ 47 kg Holstein cows equipped with rumen fistulas from Sunchon National University farm.
  • Hanwoo a domestic animal, is Italian ly grass and rich feed (55% corn, 15% wheat, 8% skim rice, 5% corn gluten feed, 10% soybean meal, 0.2% molasses, calcium carbonate (limestone) ) 2.0%, salt 0.5%, calcium phosphate 1.3%, vitamin-mineral mixture (vitamin A 3000 IU, vitamin D 6000 IU, vitamin E 30 IU, Cu 25 mg, Fe 150 mg, Zn 200 mg, Mn 100 mg, Co 0.5 mg, and I 1.5 mg) 1.0%) was fed twice daily to 2% of body weight in a ratio of 2: 8 and free water was taken.
  • Buffer (Hino et al., 1992) is K 2 HPO 4 0.45 g / L , KH 2 PO 4 0.45 g / L, (NH 4) 2 SO 4 0.9 g / L, CaCl 2 ⁇ 2H 2 O 0.12 g / L , Basal media comprising MgSO 4 7H 2 O 0.19 g / L, Trypticase 1.0 g / L, yeast extract 1.0 g / L, cysteine HCl 0.6 g / L (pH 6.9) was prepared.
  • Rumen and buffer were mixed at a ratio of 1: 3 (rumen: buffer) and filled with nitrogen gas (N 2 gas).
  • N 2 gas nitrogen gas
  • Each fermented feed was placed in a 160 ml serum bottle with 2% dry matter, 100 ml of the prepared buffer was kept anaerobic with O 2 free-N 2 , sealed with a rubber stopper and an aluminum cap, and The mixture was incubated at 100 rpm in an anaerobic state (Hattori and Matsui, Anaerobe, Vol. 14, pp. 87-93, (2008)).
  • In vitro culture was performed in three replicate experiments for 0, 12, 24, and 48 hours, and pH, total gas evolution, methane, ammonia, and VFA were measured as characteristics of the rumen fermentation.
  • the pH was not measured directly in the incubator but stabilized at the same temperature as room temperature, and then measured using an M503P meter (wrks, Medififield, MA, USA).
  • the total gas generation amount was stabilized and used EA-6 (Inc, Sun Bee instrument) pressure sensor measuring instrument. After the total gas generation was measured, the generated gas was collected using a vacuum tube to measure the amount of methane and carbon dioxide generated. On the basis of the total gas generation amount obtained for each incubation time, the gas generation amount was estimated by the formula of Qrskov and McDonald (1979).
  • the VFA measurement was analyzed after centrifugation of the culture by time incubation at 1000 ⁇ g 4 °C for 10 minutes to extract the supernatant and purified with a 0.2 ⁇ m micro filter.
  • HPLC analysis was carried out at 35 ° C. using HPLC (Agilent technolgies 1200 series) equipped with a METACARB87H (Varian, Germany) column, and the UV wavelengths of the detectors were 210 nm and 220 nm.
  • the mobile phase solvent was 0.0085 NH 2 SO 4 and the flow rate was 0.6 ml / min.
  • Ammonia concentration was measured by centrifuging the sample at 13000rpm and spectroscopy was carried out by developing ammonia in the sample with phenol solution according to the method of Chany and Marbach (Clinical Chemistry, Vol. 8, pp. 130-132, (1962)). Absorbance was measured and measured at 630 nm using a photometer (Spectronics 21D).
  • Figure 2 shows the dry matter loss of the fermented feed according to the incubation time.
  • a combination of bran and brew by-products or mushroom by-products was used as fermented feed.
  • the amount of loss in mushroom by-products was higher than for brewing by-products.
  • mushroom byproducts are easier to digest in the rumen than brewing byproducts.
  • Silage provides for feed quality and maximum building. Microorganisms in the feed also speed up the fermentation and enhance the results of silage. Therefore, the fermentation effect was measured in vitro by adding microorganisms having propionic acid production capacity.
  • Table 4 shows the dry matter (buildings) loss with fermentation incubation time.
  • the loss of building by mushroom by-products was lower than by brewing by-products (P ⁇ 0.05).
  • the increase in dry matter loss of brewing by-products of silage is indicated by the addition to Lactobacillus mucosa.
  • Table 5 shows the VFA production by fermentation. Dry matter loss was observed to be low in mushroom byproducts, while total VFA and propionic acid were high in brewing byproducts. In addition, fermentation by adding Lactobacillus mucosa to the by-product during the treatment showed high propionic acid production and total volatile fatty acids (TVFA). This shows that the acid change of mushroom by-products occurs higher than brewing by-products due to nutritional composition. The large amount of acid produced while silage is produced means that feed is a higher energy source for ruminants.
  • Table 6 shows the total gas production, pH, and ammonia nitrogen production by in vitro fermentation of brewing by-products and mushroom by-products.

Abstract

Cette invention concerne un micro-organisme ayant une capacité de production d'acide propionique et une composition alimentaire le contenant.
PCT/KR2014/007770 2013-08-30 2014-08-21 Micro-organisme ayant une capacité de production d'acide propionique et composition alimentaire le contenant WO2015030423A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2016538843A JP2016533755A (ja) 2013-08-30 2014-08-21 プロピオン酸の産生能を持つ微生物及びそれを含む粗飼料組成物

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20130104520A KR101470995B1 (ko) 2013-08-30 2013-08-30 프로피온산 생산능을 갖는 미생물 및 그를 포함하는 조사료 조성물
KR10-2013-0104520 2013-08-30

Publications (1)

Publication Number Publication Date
WO2015030423A1 true WO2015030423A1 (fr) 2015-03-05

Family

ID=52586905

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2014/007770 WO2015030423A1 (fr) 2013-08-30 2014-08-21 Micro-organisme ayant une capacité de production d'acide propionique et composition alimentaire le contenant

Country Status (3)

Country Link
JP (1) JP2016533755A (fr)
KR (1) KR101470995B1 (fr)
WO (1) WO2015030423A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019506413A (ja) * 2016-02-25 2019-03-07 カエルス ファーマシューティカルズ ビー．ヴィ． ビタミンｂ１２欠乏症を予防および／または治療するための組成物および方法
CN113575768A (zh) * 2021-06-23 2021-11-02 中国农业科学院北京畜牧兽医研究所 用于调控反刍动物瘤胃发酵气体产量的组合物及其用途

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040048356A1 (en) * 2000-11-10 2004-03-11 Marie-Louise Johansson Use of a strain of lactobacillus reducing the risk factors involved in the metabolic syndrome
KR100575522B1 (ko) * 2003-11-11 2006-05-03 대한민국 옥수수 사일리지 발효용 미생물 첨가제
US20090162322A1 (en) * 2004-05-28 2009-06-25 Markus Rudolph Oral administration form comprising probiotic bacteria
KR20110102172A (ko) * 2010-03-08 2011-09-16 김중관 젖소 또는 육우용 혼합-발효사료
US20130084623A1 (en) * 2010-06-16 2013-04-04 Instytut Biotechnologii Przemysul Ronlno-Spozywczego Strain of lactobacillus plantarum s, the use of the strain of lactobacillus plantarum s and the preparation for roughages ensiling

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080138461A1 (en) * 2006-12-11 2008-06-12 Pioneer Hi-Bred International, Inc. Lactobacillus buchneri strain LN1297 and its use to improve aerobic stability of silage
WO2008078878A1 (fr) * 2006-12-22 2008-07-03 Deuk-Sik Lee Aliments fermentés pour l'élevage de bétail à l'aide de bactéries d'acide lactique et de levures et procédé de traitement associé
JP2009044976A (ja) * 2007-08-16 2009-03-05 Meiji Shiryo Kk 動物の体型改良飼養システム
JP2011030466A (ja) * 2009-07-30 2011-02-17 Kamada Kogyo:Kk 肉牛飼料及びそれを用いた肉牛の肥育方法
WO2011071134A1 (fr) * 2009-12-10 2011-06-16 明治乳業株式会社 Composition prophylactique pour infection grippale
AU2010336620B2 (en) * 2009-12-25 2016-02-04 Meiji Feed Co., Ltd. Composition containing bacterium capable of producing propionic acid bacterium, and use thereof
US20120034198A1 (en) * 2010-08-04 2012-02-09 Microbios, Inc. Carriers for storage and delivery of biologics

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040048356A1 (en) * 2000-11-10 2004-03-11 Marie-Louise Johansson Use of a strain of lactobacillus reducing the risk factors involved in the metabolic syndrome
KR100575522B1 (ko) * 2003-11-11 2006-05-03 대한민국 옥수수 사일리지 발효용 미생물 첨가제
US20090162322A1 (en) * 2004-05-28 2009-06-25 Markus Rudolph Oral administration form comprising probiotic bacteria
KR20110102172A (ko) * 2010-03-08 2011-09-16 김중관 젖소 또는 육우용 혼합-발효사료
US20130084623A1 (en) * 2010-06-16 2013-04-04 Instytut Biotechnologii Przemysul Ronlno-Spozywczego Strain of lactobacillus plantarum s, the use of the strain of lactobacillus plantarum s and the preparation for roughages ensiling

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019506413A (ja) * 2016-02-25 2019-03-07 カエルス ファーマシューティカルズ ビー．ヴィ． ビタミンｂ１２欠乏症を予防および／または治療するための組成物および方法
CN113575768A (zh) * 2021-06-23 2021-11-02 中国农业科学院北京畜牧兽医研究所 用于调控反刍动物瘤胃发酵气体产量的组合物及其用途
CN113575768B (zh) * 2021-06-23 2023-09-15 中国农业科学院北京畜牧兽医研究所 用于调控反刍动物瘤胃发酵气体产量的组合物及其用途

Also Published As

Publication number Publication date
KR101470995B1 (ko) 2014-12-09
JP2016533755A (ja) 2016-11-04

Similar Documents

Publication Publication Date Title
Konstantinov et al. Microbial diversity studies of the porcine gastrointestinal ecosystem during weaning transition
RU2528859C2 (ru) Изолированный штамм (варианты), обеспечивающий улучшение состояния здоровья жвачных животных, способ его получения, и способ его введения жвачным животным
CN109929773B (zh) 一株可用于富硒培养的双歧杆菌及其活性蛋白和应用
Miguel et al. Enhancing butyrate production, ruminal fermentation and microbial population through supplementation with Clostridium saccharobutylicum
JP6663977B2 (ja) バチルスサブチリスとバチルスリケニフォルミスを含む飼料添加剤、前記添加剤を含む飼料組成物及び前記飼料添加剤の製造方法
Kim et al. Fumarate reductase-producing enterococci reduce methane production in rumen fermentation in vitro
WO2019027073A1 (fr) Additif d'aliments pour animaux contenant bacillus subtilis et bacillus licheniformis, composition d'aliments pour animaux contenant ledit additif et procédé de préparation dudit additif
Sadet-Bourgeteau et al. Equine microbial gastro-intestinal health
CN113403227A (zh) 一株植物乳杆菌及其制备方法和应用
WO2015030423A1 (fr) Micro-organisme ayant une capacité de production d'acide propionique et composition alimentaire le contenant
Azzaz et al. A newly developed strain of Enterococcus faecium isolated from fresh dairy products to be used as a probiotic in lactating Holstein cows
CN107012095B (zh) 猪结肠微生物组体外模拟培养的发酵参数
CN116445372B (zh) 提高反刍动物瘤胃氮素转化效率的敏捷乳杆菌及其应用
WO2017222142A1 (fr) Souche ny1505 de bacillus licheniformis pour la production en masse d'un inhibiteur d'α-glucosidase
KR100631857B1 (ko) 감마아미노부틸산(ｇａｂａ) 고생산성 균주인락토바실러스 브레비스 오피케이-3
CN109423466A (zh) 一种复合发酵菌剂及其应用
JP2016533755A5 (fr)
KR20080011901A (ko) 내산성, 내담즙성의 적응력이 강하고, 병원성 미생물의 생육을 억제하고, 알파-갈락토시데이지 효소를 생산하는 락토바실러스 살리바리우스 에스피 살리바리우스 df20(kctc10942bp) 미생물
Stewart Lactic acid bacteria in the rumen
SUHARTI et al. Morphological, biochemical, and molecular identification of cellulolytic bacteria isolated from feces of endemic tropical herbivores
Onrust et al. Caecibacterium sporoformans gen. nov., sp. nov., an anaerobic, butyrate-producing, spore-forming bacterium isolated from chicken caecum
Marounek et al. Metabolism and some characteristics of lactobacilli isolated from the rumen of young calves
Fujisawa et al. Influences of Prebio SupportTM (mixture of fermented products of Lactobacillus gasseri OLL2716 and Propionibacterium freudenreichii ET-3) on the composition and metabolic activity of fecal microbiota in calves
CN117070419B (zh) 源于奶牛瘤胃的栖瘤胃肠球菌及其作为饲料添加剂的应用
CN112493366B (zh) 一种固态发酵断奶仔猪饲料的复合菌剂、制备方法及应用

Legal Events

Date Code Title Description
ENP Entry into the national phase

Ref document number: 2016538843

Country of ref document: JP

Kind code of ref document: A

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 14840685

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 14840685

Country of ref document: EP

Kind code of ref document: A1