WO2015021058A3 - Engineered proteins with a protease cleavage site - Google Patents

Engineered proteins with a protease cleavage site Download PDF

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Publication number
WO2015021058A3
WO2015021058A3 PCT/US2014/049805 US2014049805W WO2015021058A3 WO 2015021058 A3 WO2015021058 A3 WO 2015021058A3 US 2014049805 W US2014049805 W US 2014049805W WO 2015021058 A3 WO2015021058 A3 WO 2015021058A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
cleavage site
recombinant
protease cleavage
engineered proteins
Prior art date
Application number
PCT/US2014/049805
Other languages
French (fr)
Other versions
WO2015021058A2 (en
Inventor
William Jeremy BLAKE
Drew S. CUNNINGHAM
Original Assignee
Greenlight Biosciences, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to JP2016533377A priority Critical patent/JP6483687B2/en
Priority to DK14752728.7T priority patent/DK3030671T3/en
Priority to AU2014306074A priority patent/AU2014306074B2/en
Priority to EP18197684.6A priority patent/EP3460069B1/en
Priority to EP14752728.7A priority patent/EP3030671B1/en
Priority to CA2919383A priority patent/CA2919383A1/en
Priority to BR112016002494A priority patent/BR112016002494A2/en
Priority to ES14752728T priority patent/ES2704697T3/en
Application filed by Greenlight Biosciences, Inc. filed Critical Greenlight Biosciences, Inc.
Priority to KR1020167004738A priority patent/KR20160037194A/en
Priority to CN201480044309.9A priority patent/CN105658807A/en
Priority to SG11201600898YA priority patent/SG11201600898YA/en
Publication of WO2015021058A2 publication Critical patent/WO2015021058A2/en
Publication of WO2015021058A3 publication Critical patent/WO2015021058A3/en
Priority to IL243641A priority patent/IL243641A0/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01001—Transketolase (2.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01008—Phosphate acetyltransferase (2.3.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01009—Glucose-6-phosphate isomerase (5.3.1.9)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Various aspects and embodiments herein relate to recombinant proteins with at least one protease recognition sequence that can be inactivated by a cognate protease and methods of preparing such proteins. In some embodiments, recombinant phosphoglucose isomerase (Pgi) proteins are provided. In other embodiments, recombinant phosphotransacetylase (Pta) proteins are provided. In yet other embodiments, recombinant transketolase A (TktA) proteins are provided.
PCT/US2014/049805 2013-08-05 2014-08-05 Engineered proteins with a protease cleavage site WO2015021058A2 (en)

Priority Applications (12)

Application Number Priority Date Filing Date Title
BR112016002494A BR112016002494A2 (en) 2013-08-05 2014-08-05 PROTEINS CONSTRUCTED WITH A PROTEASE CLEAVAGE SITE, NUCLEIC ACID, VECTOR, CELL AND PROCESS ENGINEERING A RECOMBINANT PROTEIN AND A LARGE NUMBER OF NUCLEIC ACID VARIANTS THAT ENCODE RECOMBINANT PROTEINS
AU2014306074A AU2014306074B2 (en) 2013-08-05 2014-08-05 Engineered proteins with a protease cleavage site
EP18197684.6A EP3460069B1 (en) 2013-08-05 2014-08-05 Therapeutically active compounds and their methods of use
EP14752728.7A EP3030671B1 (en) 2013-08-05 2014-08-05 Engineered proteins with a protease cleavage site
CA2919383A CA2919383A1 (en) 2013-08-05 2014-08-05 Engineered proteins with a protease cleavage site
JP2016533377A JP6483687B2 (en) 2013-08-05 2014-08-05 Engineered proteins with protease cleavage sites
ES14752728T ES2704697T3 (en) 2013-08-05 2014-08-05 Genomodified proteins with a protease cleavage site
DK14752728.7T DK3030671T3 (en) 2013-08-05 2014-08-05 MODIFIED PROTEINS WITH A PROTEASE CLEANING PLACE
KR1020167004738A KR20160037194A (en) 2013-08-05 2014-08-05 Engineered proteins with a protease cleavage site
CN201480044309.9A CN105658807A (en) 2013-08-05 2014-08-05 Engineered proteins with protease cleavage site
SG11201600898YA SG11201600898YA (en) 2013-08-05 2014-08-05 Engineered proteins with a protease cleavage site
IL243641A IL243641A0 (en) 2013-08-05 2016-01-17 Engineered proteins with a protease cleavage site

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361862363P 2013-08-05 2013-08-05
US61/862,363 2013-08-05
US201461987518P 2014-05-02 2014-05-02
US61/987,518 2014-05-02

Publications (2)

Publication Number Publication Date
WO2015021058A2 WO2015021058A2 (en) 2015-02-12
WO2015021058A3 true WO2015021058A3 (en) 2015-04-16

Family

ID=51358108

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2014/049805 WO2015021058A2 (en) 2013-08-05 2014-08-05 Engineered proteins with a protease cleavage site

Country Status (13)

Country Link
US (2) US9688977B2 (en)
EP (2) EP3460069B1 (en)
JP (1) JP6483687B2 (en)
KR (1) KR20160037194A (en)
CN (1) CN105658807A (en)
AU (1) AU2014306074B2 (en)
BR (1) BR112016002494A2 (en)
CA (1) CA2919383A1 (en)
DK (1) DK3030671T3 (en)
ES (1) ES2704697T3 (en)
IL (1) IL243641A0 (en)
SG (1) SG11201600898YA (en)
WO (1) WO2015021058A2 (en)

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EP2566953B1 (en) 2010-05-07 2019-01-02 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through enzyme relocation
JP6280367B2 (en) 2010-08-31 2018-02-14 グリーンライト バイオサイエンシーズ インコーポレーテッドＧｒｅｅｎｌｉｇｈｔ Ｂｉｏｓｃｉｅｎｃｅｓ，Ｉｎｃ． A method for the control of fluxes in metabolic pathways via protease manipulation
SG2014014377A (en) 2011-09-09 2014-05-29 Greenlight Biosciences Inc Cell-free preparation of carbapenems
EP2935598A1 (en) 2012-12-21 2015-10-28 Greenlight Biosciences Inc. Cell-free system for converting methane into fuel, pyruvate or isobutanol
JP6483687B2 (en) 2013-08-05 2019-03-13 グリーンライト バイオサイエンシーズ インコーポレーテッドＧｒｅｅｎｌｉｇｈｔ Ｂｉｏｓｃｉｅｎｃｅｓ，Ｉｎｃ． Engineered proteins with protease cleavage sites
MY193444A (en) 2015-03-30 2022-10-13 Greenlight Biosciences Inc Cell-free production of ribonucleic acid
SG11201808721YA (en) 2016-04-06 2018-11-29 Greenlight Biosciences Inc Cell-free production of ribonucleic acid
JP7186167B2 (en) 2017-01-06 2022-12-08 グリーンライト バイオサイエンシーズ インコーポレーテッド Cell-free production of sugar
BR102017026619A2 (en) * 2017-02-15 2018-10-30 Euroimmun Medizinische Labordiagnostika Ag improved assay for peanut allergy diagnosis
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