WO2014204423A1 - Crème cosmétique - Google Patents

Crème cosmétique Download PDF

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Publication number
WO2014204423A1
WO2014204423A1 PCT/UA2014/000063 UA2014000063W WO2014204423A1 WO 2014204423 A1 WO2014204423 A1 WO 2014204423A1 UA 2014000063 W UA2014000063 W UA 2014000063W WO 2014204423 A1 WO2014204423 A1 WO 2014204423A1
Authority
WO
WIPO (PCT)
Prior art keywords
skin
polypeptides
cosmetic cream
plant
cosmetic
Prior art date
Application number
PCT/UA2014/000063
Other languages
English (en)
Russian (ru)
Inventor
Зураб Давидович ГОГИТИДЗЕ
Original Assignee
Gogitidze Zurab Davidovich
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from UAU201307841U external-priority patent/UA83294U/ru
Application filed by Gogitidze Zurab Davidovich filed Critical Gogitidze Zurab Davidovich
Publication of WO2014204423A1 publication Critical patent/WO2014204423A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q7/00—Preparations for affecting hair growth

Definitions

  • the claimed invention relates to the field of cosmetics, in particular, to skin care products and can be used to obtain cosmetic creams that help rejuvenate human skin, smooth wrinkles, restore normal skin condition, in particular, its moisture, elasticity, strength, elasticity, natural color and smoothness.
  • cosmetics are known to enhance the appearance of the skin.
  • cosmetics are known, the use of which makes it possible to accumulate and retain moisture in the skin cell, which for some time eliminates or prevents dryness and loss of skin elasticity.
  • antioxidants that bind free radicals that are created in the skin, or prevent the creation of new ones, which as a result leads to the prevention of reactions that adversely appear on the normal state of the cell.
  • drugs which contain ascorbic acid or vitamin E as antioxidants (Hoick David E.E., John D. Ng. Facial skin rejuvenation. Curr Qpin in Ophthalmol 14: 249).
  • retinoids Hoick David E.E., John PN Facial skin rejuvenation, Curr Opin in Qphthalmol 14: 248
  • stimulating the activity of fibroblasts and the synthesis of new collagen fibers in the dermis as well as mitotic activity and cell renewal in the epidermis, which leads to a decrease in the thickness of the stratum corneum, an increase in the thickness of the granular layer of the skin, and an improvement in the structure of the skin.
  • the disadvantages of the known cosmetics is that they, as a rule, give only a negligible effect regarding the improvement of some functions of the skin and its appearance, without affecting, at the same time, the aging processes of skin cells and ensuring its vital functions.
  • the aim of the invention is the creation of a cosmetic cream, which includes only components of plant origin, both the active ingredient and the base, the complex effect of which is aimed at rejuvenating human skin, smoothing wrinkles, restoring normal skin condition, in particular, its moisture, elasticity, strength, elasticity, natural color and smoothness.
  • this goal is achieved by developing a new cosmetic cream containing the active component and the carrier, where the active component consists of biologically active plant-derived polypeptides of molecular weight from 1 to 98 ka, and the carrier is the basis of plant origin, in the following ratio of components, mass %: active component - 5 - 40, the carrier - the rest.
  • the prerequisites for the creation of a cosmetic product of plant origin are studies of the plant immune system consisting of low molecular weight polypeptides. Such polypeptides are recognized by the human immune system as “their own,” compared with animal polypeptides that are only partially absorbed by the human body.
  • each layer of human skin in particular, the epidermis, dermis and hypodermis, plays its protective and special function, and, in addition, has significant differences from each other in composition and structure, the polypeptides that form the active component of the cosmetic beneficial effect on all these layers of the skin.
  • the effect of cosmetic cream on the outer layer of the epidermis is to support the activity and activity of special lipids covering each cell of the outer ball of the epidermis, block moisture and prevent its evaporation, which helps to maintain moisture and elasticity of each epidermal cell.
  • the polypeptides that make up the cosmetic cream contribute to the active formation of collagen, consisting of the amino acids that make up the polypeptides, which ensures the strength and elasticity of human connective tissue. Since with age (on average after 25 years), the production of own collagen by fibroblasts noticeably decreases, which leads to degeneration of collagen fibers, and this in turn affects the skin condition - a decrease in skin elasticity, the appearance of wrinkles, and deterioration of skin color, then application to human skin additional “building material” in the form of polypeptides significantly improves the productivity and activity of fibroblasts.
  • the cosmetic cream also has a similar effect on elastin fibers, which provide elasticity to the fibers of the human connective tissue.
  • the most suitable carrier is a carrier of plant origin. It is these carriers that efficiently deliver the polypeptides that make up the active ingredient to all the necessary corners of human skin.
  • the created cosmetic product in the form of a complex of plant origin with its effective action promotes the rejuvenation of human skin, smoothing wrinkles, restoring the normal condition of the skin, in particular, its moisture, elasticity, strength, elasticity, natural color and smoothness.
  • FIG. 1 is a diagram of the separation of drug polypeptides in 10% PAG
  • FIG. 2 A, 2 B image of a fragment of the skin of the patient before and after using cosmetic cream
  • FIG. 3 A, 3 B image of a fragment of the skin of the patient before and after using cosmetic cream
  • Methods for producing polypeptides from plant materials are known in the art.
  • the known method may comprise the following steps: sterilization, washing, drying, grinding to a powder state, extraction, filtration.
  • the polypeptide composition of the preparation is determined (also using methods known in the art).
  • the preparation was diluted in buffered saline (PBS) and passed through a desalting microcentrifuge column (Pierce), prepared according to the manufacturer's instructions.
  • PBS buffered saline
  • ierce desalting microcentrifuge column
  • Electrophoretic separation of polypeptides was carried out on plates with a 4.5% concentrating and 10% separating polyacrylamide gel in the presence of sodium dodecyl sulfate (SDS) in a Lemmley buffer system according to the standard procedure using a Mini Protean 3 camera (Biorad). Electrophoresis was performed at a current of 10 mA to and 30 mA after the samples entered the separating gel.
  • SDS sodium dodecyl sulfate
  • the resulting gels were washed from SDS for 5 minutes with water and fixed for 10 minutes in a mixture of 50% methanol, 10% acetic acid and 40% water.
  • the gels were stained for 1 hour in exactly the same mixture, optionally containing 0.2% Coomassie R-250 dye.
  • the background in the stained gels was washed with several shifts of a mixture of 45% methanol, 10% acetate and 45% water.
  • the resulting gels with visible polypeptide fractions were scanned using the G: BOX gel documentation system (Syngene).
  • Peptide mixtures were analyzed by high performance liquid chromatography (HPLC) followed by tandem mass mass spectrometry using an LTQ FT ICR mass spectrometer (Hybrid-2D-Linear Quadrupole Ion Trap, Fourier Transform Ion Cyclotron Resonance Mass Spectrometer) (Thermo Electron Corp). Before analysis, the mixture was subjected to the standard procedure of recovering 100 mM DTT for 30 minutes at 56 ° C, alkylating with iodoacetamide for 45 minutes at room temperature in the dark, and partially digesting with modified trypsin for sequencing (Promega) overnight.
  • HPLC high performance liquid chromatography
  • LTQ FT ICR mass spectrometer Hybrid-2D-Linear Quadrupole Ion Trap, Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
  • the resulting output was converted using the Mascot Distiller software and used for subsequent searches using the Mascot software (Matrix Science) against a non-degenerate NCBI database.
  • the preparation contains several minor fractions of polypeptides with visible molecular weights ranging from 1 to 98 kDa. Protein fractions of molecular weights are plotted on the middle lane (M).
  • a significant part of the known polypeptides are various additional plant proteins, for example, legumin and / or legumin-like proteins, reserve globulins, cruciferin, glutelin, as well as plant proteins with enzymatic activity, such as, for example, phosphoglycerate kinase, SDR dehydrogenase / reductase, fructose bisphosphate aldolase, superoxide dismutase, malate dehydrogenase, glutamate dehydrogenase, beta-amylase, glyoxalase, etc.
  • plant proteins with enzymatic activity such as, for example, phosphoglycerate kinase, SDR dehydrogenase / reductase, fructose bisphosphate aldolase, superoxide dismutase, malate dehydrogenase, glutamate dehydrogenase, beta-amylase, glyoxalase, etc.
  • an emulsified base was developed.
  • the composition of the oil phase (25%) was introduced only natural fats, waxes and oils, which, in addition to the formative function, themselves provide the life-giving effect of the skin.
  • a complex emulsifier was chosen (a mixture of emulsifiers I and P) and a solution of high molecular weight substance (IUD) - sodium alginate.
  • an IUD into the aqueous dispersion medium makes it possible to increase the stability of the emulsion at a low concentration of emulsifiers (the negative effect of emulsifiers is manifested in a decrease in the epidermal barrier of the skin, namely, destruction of the aqueous lipid membrane and corneal lipids of the epidermis).
  • a mixture of fatty oils apricot oil, wheat germ oil;
  • the active component in the form of a solution was introduced into the composition of the prepared base: 1 mg of the active component in the form of a lyophilized powder was diluted in 5 ml of water for injection; the solution was emulsified with 95 h of base and 100 g of agent was obtained.
  • the drug was packaged in 5 plastic jars for creams of 20 g. The amount of 20 g was intended for use within 5 days. The study lasted 20 days, so the drug was prepared 4 times.
  • the cream has an appropriate consistency, medium fat content, is well absorbed into the skin; after 10 days of use, an improvement in skin color, a decrease in the size of fine wrinkles, and an increase in skin elasticity were observed.
  • Example 2 The experiment involved 20 women of different age groups (from 35 to 60 years) with different skin types. All women experienced such manifestations of aging as flabbiness and wrinkles, dryness, thinning and sagging skin, photo pigmentation.
  • polypeptides do not paralyze muscles and do not violate the natural facial expressions, but gently suppress and reduce the contraction of facial muscles, which leads to their relaxation, prevents the formation of new facial wrinkles and helps smooth out existing wrinkles and fine lines.
  • An additional advantage of a cosmetic cream with this composition is the fact that it can be applied to any part of the body.
  • cosmetics based on polypeptides • Increase the protective functions of the skin, the resistance of cells to oxygen starvation and the action of toxins, help maintain the necessary activity of cellular systems, which leads to better protection of the skin from the harmful effects of environmental factors.
  • the claimed cosmetic cream can be used in cosmetology to improve the appearance of human skin.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Dermatology (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Cosmetics (AREA)

Abstract

L'invention concerne le domaine de la cosmétique, notamment des soins de la peau, et peut s'utiliser pour produire des crèmes cosmétiques favorisant le rajeunissement de la peau de la personne, la disparition des rides, le rétablissement de l'état normal de la peau, notamment de son humidité, de sa souplesse, de sa résistance, de son élasticité, de sa couleur naturelle et de son aspect lisse. La crème cosmétique comprend un principe actif et un excipient, le principe actif étant composé des polypeptides biologiquement actifs d'origine végétale possédant une masse moléculaire de 1 à 98 kilodaltons, l'excipient se présentant comme une base d'origine végétale, avec le rapport suivant des composés, en masse: % principe actif - 5 - 40, le reste est constitué par l'excipient.
PCT/UA2014/000063 2013-06-19 2014-06-13 Crème cosmétique WO2014204423A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
UAU201307841U UA83294U (en) 2013-06-19 2013-06-19 Cosmetic cream
UAU201307841 2013-06-19
UA201307848 2013-06-20
UAA201307848 2013-06-20

Publications (1)

Publication Number Publication Date
WO2014204423A1 true WO2014204423A1 (fr) 2014-12-24

Family

ID=52105005

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/UA2014/000063 WO2014204423A1 (fr) 2013-06-19 2014-06-13 Crème cosmétique

Country Status (1)

Country Link
WO (1) WO2014204423A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009136805A1 (fr) * 2008-05-04 2009-11-12 Общество С Ограниченной Ответственностью "Makeha Рус" Agent immunostimulant et procédé de production correspondant
WO2011146036A1 (fr) * 2010-05-18 2011-11-24 Gogitidze Zurab Davidovych Produit médicamenteux à action protectrice sur multiples organes pour traiter des états dus à un disfonctionnement cellulaire
CN102727402A (zh) * 2011-04-06 2012-10-17 杨兰花 含有稻米多肽的化妆品
FR2982772A1 (fr) * 2011-11-22 2013-05-24 Limousine D Applic Biolog Soc Ind Utilisation de peptides obtenus a partir d'alisma comme agent cosmetique pour renforcer la fonction barriere globale de la peau

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009136805A1 (fr) * 2008-05-04 2009-11-12 Общество С Ограниченной Ответственностью "Makeha Рус" Agent immunostimulant et procédé de production correspondant
WO2011146036A1 (fr) * 2010-05-18 2011-11-24 Gogitidze Zurab Davidovych Produit médicamenteux à action protectrice sur multiples organes pour traiter des états dus à un disfonctionnement cellulaire
CN102727402A (zh) * 2011-04-06 2012-10-17 杨兰花 含有稻米多肽的化妆品
FR2982772A1 (fr) * 2011-11-22 2013-05-24 Limousine D Applic Biolog Soc Ind Utilisation de peptides obtenus a partir d'alisma comme agent cosmetique pour renforcer la fonction barriere globale de la peau

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