WO2014166483A1

WO2014166483A1 - Method for producing and an egg oil composition

Info

Publication number: WO2014166483A1
Application number: PCT/DE2014/100126
Authority: WO
Inventors: Werner Ch. Nawrocki
Original assignee: Nawrocki Werner Ch
Priority date: 2013-04-12
Filing date: 2014-04-10
Publication date: 2014-10-16
Also published as: EP2986145A1; CN105263337A; DE112014001928A5

Abstract

The invention relates to a method for producing an egg oil composition from avian or reptilian egg yolk for the treatment of skin burns and skin diseases.

Description

Process for the preparation of an egg oil composition

The present invention relates to a process for producing an egg oil composition from egg yolk of birds or reptiles.

In burns and wounds of all kinds, both open and closed, are now used worldwide mainly active ingredients that either have many side effects or require strong preservatives due to lack of decomposition stability of the active ingredients used. This also applies to preparations for the regeneration of the skin and / or, for example, ointments for the treatment of scars, especially in plastic reconstructive surgery. In addition, antibiotics often have to be used because superinfections with Gram-negative bacteria often occur.

In recent years, especially in cases of burns and wound healing of the skin in medicine, the ineffectiveness of antibiotics is noticeable.

It is known from the prior art, high-purity egg oil, which can be obtained from the egg yolk of birds or reptiles, for the treatment of skin burns and as an active ingredient for regenerating diseased or attacked as a result of special stress skin surfaces can be used. Thus, in WO 95/22590 of the applicant a process for the preparation of a high-purity egg oil and its use in the treatment of burns and skin diseases is described.

The effect of egg oil (brand name: Charismon®) can be described scientifically, biophysically as follows: The purely biochemical analysis of egg oil shows saturated and unsaturated fatty acids (PUFA) on. These include both Omega 3 and Omega 6 fatty acids. Of these, the following effect is shown by the formation of eicosanoids:

- Regulation of lipolysis

- Resolution of inflammatory processes

- Regulation of ion transport through the cell membranes

- Modulation of the synaptic potential

- Triggering the blood clotting processes

- Regulation of the sleep / wake cycle

- Control of various reproductive processes

- Regulation of cholesterol synthesis

- Improving susceptibility to infection

- Fine tuning the mood

- influence on the memory and the feelings

- age-related vision loss

- brain activity

- Brain and eyesight of toddlers

- Growth

Egg oil has good external and internal efficacy on the skin and mucous membranes, and obviously on every cell. It also prolongs cell apoptosis (cell death).

It is also known today that these effects on the emotional situation of the omega-3 and omega-6 fatty acids can be achieved through the transformation to the eicosanoids.

Egg oil has no side effects. The production of egg oil is - as stated above - in the patented method of Dr. med. W.Ch. Nawrocki, z. As described in WO 95/22590. The content of said patent application is expressly also content of this patent application.

In the Hermetic philosophy as the theoretical superstructure of the Spagyrik and Alchemy assumes that everything in the world of material manifestation consists of the three essential constituents, body, soul and spirit (KSG).

The body is the bearer of material form, the soul is the bearer of information, and the mind is the bearer of energy. The spagyric, alchemically produced egg oil is therefore the carrier of the information. Symbolically speaking, the egg oil is also the carrier of the life information from the yolk. That is why it is also called the quintessence. The basic procedure of spagyric and alchemy means separation, purification and reunification (separation, purificatio and cohabitation). The egg oil, Charismon®, is the carrier of the information here. The life energy is still present in the egg oil.

Therefore, the spagyrist and alchemist now separates the carrier of the life energy, the carrier of the spirit, from the yolk by another process from the pure egg oil.

Because the egg oil has already undergone a separation and purification, is therefore spagyric, alchemically pure and high quality.

The inventor of the above-mentioned method for producing egg oil has set itself the object underlying the present invention to increase the effectiveness of the egg oil obtained by said method yet.

The hitherto known process for obtaining high-purity egg oil from the egg yolk of birds or reptiles is characterized by the following process steps: a) drying the egg yolk at temperatures up to 90 ° C. and comminuting to form a powdery product (A);

b) extracting the product (A) from step (a) for a period of three to seven days with a fatliquoring extractant;

c) slowly distilling off the extractant to form a viscous residue (B);

d) aging the residue (B) of step (c) at ambient temperature for a period of up to 10 hours, preferably for five to seven hours; e) further aging of the residue (B) at a temperature of between 7 and 12 ° C for a period of up to 24 hours, until a distinct phase separation occurs; f) separating the lower viscosity phase (C) formed in the phase separation in step (e);

g) centrifuging the lower viscosity product (C) from step (f) for 20 to 40 minutes at a rate of 900 to 1200 revolutions per minute, and separating the lower viscosity phase (D);

h) mixing the product (D) from step (g) with demineralized water in the volume ratio of 1: 2 to 1: 5 and heating the mixture for a period of 30 to 90 minutes to a temperature of 90 to 120 ° C, preferably 95 to 100 ° C;

i) cooling the aqueous mixture of step (h) to ambient temperature and separating the lower-viscosity part (E);

j) centrifuging the product (E) from step (i) for 20 to 40 minutes, at a rate of 900 to 1200 revolutions per minute and separating the lower viscosity phase (F);

k) maintaining the product (F) of step (i) at temperatures of 7 to 12 ° C for a period of up to 24 hours, until significant phase separation occurs, and separating the lower viscosity phase (G);

1) Centrifuge the product (G) from step (k) for 20 to 40 minutes at a rate of 900 to 1200 revolutions per minute and separate the lower viscosity phase (H).

In a further step, if necessary, a repetition of the measures of steps (h) to (1) or (j) to (1) with the product (H) of step (1) can be carried out.

The above-mentioned object is achieved, in particular, by further treating the egg oil obtained by the above-mentioned method as follows: m) boiling the egg oil obtained with multi-distilled water for a period of at least 20, preferably about 30 to 60 minutes; n) allow to cool to room temperature;

o) distilling off the water;

p) allowing the distillation residue to stand with ethanol for at least about one minute, preferably at least one hour at a temperature not more than a little short of the boiling point of ethanol (e.g., with stirring);

q) distilling off the ethanol;

r) mixing the distillate (alcoholic tincture), preferably after cooling to a maximum of 4 ° C with egg oil according to step (1) of the above method.

After mixing, therefore, the remaining alcohol which does not mix with the oil is slowly and carefully distilled off at very specific temperatures, which can vary from room temperature to 70 ° C (see step (q)).

This distilled alcohol is now the so-called "spirit of egg oil" (brand name: "Spirit of Charismon®") after distilling usually a certain time, preferably about one hour to three days, at a certain temperature, preferably in a temperature range from room temperature up to 30 ° C, stops.

Thereafter, the "Spirit of Charismon®" is again added to the egg oil from which it was distilled off.

This egg oil according to the invention is now a substantially different egg oil than the egg oil described in the first patent mentioned above. The effect of the egg oil produced by the process of the invention on the skin, mucous membrane is much more intense, stronger, not only in burns or wounds, but also in almost all skin problems in medicine, pharmacy, the dietary supplements, in cosmetics and personal care as well in dentistry and dental care problems such as periodontal disease. It also has other functions on the skin and especially on the microcirculation of the skin.

In addition, the pure "Spirit of Charismon" still has a very special effect on the blood vessels. It dissolves all hardening in the blood vessels, that is, it dissolves atterosclerotic plaques and thus opens again little or completely occluded vessels.

This has been observed and proven in selected cases. Larger clinical trials are already planned. For the method according to the invention:

The pure, spagyric egg oil produced by the above method in steps a) to 1) is e.g. filled in a flask.

Depending on the desired strength of the future "Spirit of Charismon®" then preferably pure, spagyric produced alcohol, in particular spagyric ethanol produced in a certain proportional ratio, preferably in the range of 1: 1 to 1:10 to the egg oil added and mixed.

The spagyric alcohol is preferably produced by plant fermentation with the help of sugar.

Then this mixture is allowed to stand in the range of 3-5 hours to 3-5 days. In between, the ratio is preferably mixed again and again according to a certain scheme, which varies in the time order and the time length of about 3-5 minutes to 7-9 minutes, since especially in the initial phase, the alcohol settles quite quickly above.

From a spagyric, alchemical point of view, the time of continuous mixing of the oil with the alcohol is very important.

The mixture according to the invention is preferably stored at temperatures of 1 to 18 ° C. for at least three days. The ethanol used is preferably medicinal alcohol. The temperature at which the distillate obtained in step o) is brought into contact with ethanol is preferably at most 77 ° C. The distillation under step (q) is preferably carried out slowly and carefully at the lowest possible temperature, in particular at ca. 78 ° C performed. Before this distillation is preferably cooled, in particular cooled to at most 40 ° C.

The inventive mixture of egg oil and alcoholic tincture of egg oil can be used directly in medicine. Preferably, the mixture according to the invention is further processed into creams or ointments. It has been found that the mixture according to the invention is even more effective than pure egg oil. Experience has shown that the mixture according to the invention is used for the regeneration of the skin after burns, wound healing in severe burns and closed as well as open wounds of the skin as well as for scar treatment in plastic surgery. Further uses according to the invention are the treatment of atopic dermatitis, psoriasis, acne and eczema.

For the method according to the present invention, the egg yolk is used by birds such as chickens, ducks, ostriches, etc., or alternatively by reptiles such as turtles as starting material.

This starting material is dried in a first process step (a) at temperatures up to a maximum of 90 ° C., preferably between 50 ° C. and 70 ° C. On an industrial scale, the production of dried egg yolk is usually done in so-called trap towers; but it also suitable drying ovens with z. B.

circulating air. The dried egg yolk is reduced to an average grain size between z. B. 0.3 and 1.0 mm crushed; For example, it is pressed through a sieve in the form of a fine mesh metal wire net (10x 10 to 30x30 mesh per cm ² ). This dried egg yolk powder (A) is then subjected to an extraction process (b) using a fat-dissolving extractant which is to last for three to seven days, but preferably lasts for four to five days. As extractants are preferably

Aliphatic alcohols (having one to five, preferably one to four carbon atoms per molecule, conveniently methanol or ethanol) and / or aliphatic ketones (having up to five carbon atoms in the molecule, conveniently acetone), and / or • - Freon, and / or

• - aliphatic ethers (containing up to seven carbon atoms in the molecule, in particular

Dialkyl ethers, such as diethyl ether, as well as cyclic ethers, in particular tetrahydrofuran), and / or alternatively

· - Carbon dioxide into consideration.

The extraction technique used may be selected from a variety of conventional laboratory techniques, alternatively a combination of several of the methods listed below may be used:

Maceration at pressures that may be either lower or moderately higher than the atmospheric pressure, and / or

• Extraction according to the Soxleth method, and / or

· Pressure extraction with carbon dioxide, and / or

• - Mild cooking under reflux.

After the extraction treatment, the extractant used in the step (c) is slowly distilled off, leaving a viscous residue (B), which is used as starting material for the further process steps.

Thereafter, in two stages (d and e) made aging this viscous residue (B), namely first at ambient temperature for a period of up to 10 hours, preferably for five to seven hours, and then at a temperature of 7 to 12 ° C for a period of up to 24 hours, until a distinct phase separation occurs.

The lower viscosity phase (C) formed during aging is separated in step (f), e.g. B. either by decantation or by means of a separator, and centrifuged for a period of preferably 20 to 40 minutes, preferably for 30 minutes, at a rate of preferably 900 to 1200 revolutions per minute. This results in a phase separation, whereupon the formed, lower viscosity phase (D), z. B. by Abdekantation or with the help of a separator, separated and further processed. The higher-viscosity phase is discarded.

The resulting, lower viscosity phase (D) is then mixed in step (h) with demineralized water, wherein preferably a volume ratio of phase D: water of between 1: 2 and 1: 5 is selected. The resulting mixture is then heated to a temperature of preferably 90 to 120 ° C, preferably 95 to 100 ° C, for a period of 30 to 90 minutes, preferably for 60 minutes. After cooling the mixture to ambient temperature, a phase separation occurs, whereupon the lower-viscosity portion (E), z. B. by decantation and / or with the aid of a separating funnel is separated (step (i)). The higher-viscosity portion is discarded.

The obtained, lower-viscosity fraction (E) is centrifuged in step (i) at a rate of preferably 900 to 1200 revolutions per minute for a period of between preferably 20 and 40 minutes, preferably 30 minutes, which in turn leads to a phase separation , The lower viscosity portion (F) is z. B. decanted or separated by means of a separator. The higher-viscosity phase is discarded.

The lower-viscosity fraction (F) is preferably maintained in step (k) for a maximum period of 24 hours at a temperature of preferably 7 to 12 ° C until a further, distinct phase separation occurs. The lower viscosity phase (G) is separated off, e.g. B. by decanting, the formed, higher-viscosity phase discarded. The lower viscosity phase (G) is centrifuged for a period between 20 and 40 minutes, preferably for 30 minutes, at a rate between 900 and 1200 revolutions per minute (step (1)). The lower viscosity phase (H) formed thereby constitutes the intermediate product (egg oil).

If appropriate, the process steps can also be carried out either from the mixing of (D) with demineralized water to the centrifugation of (G) (stages (h) to (1)), or from the centrifugation of (E) to the centrifugation of (G) ( Steps (j) to (1)) to obtain a particularly pure product (H). The Eiöl (H) is a mobile oil of, depending on the starting material used, yellow to reddish color, which can be stored without the addition of preservatives.

The present invention will now be explained in more detail by means of a representative example.

example 1

The yolks of 10 fresh chicken eggs were dried at a temperature of 60 ° C in a laboratory drying oven. The dried egg yolk was then pressed through a commercially available, hemispherical household sieve made of stainless steel wire (20 × 20 mesh per cm ² ) and thereby comminuted to an average grain size of about 0.5 mm. This dried egg yolk powder was then subjected to a five-day extraction treatment in a laboratory standard Soxhlet apparatus using 250 ml of acetone.

After the extraction treatment, the acetone used was distilled off using a rotary evaporator in a slight vacuum.

This left a viscous residue, which was then aged for six hours at ambient temperature and then for 24 hours in a tempered cold room at 10 ° C. Towards the end of aging a clear phase separation was observed.

The lower viscosity phase was then decanted off and the higher viscosity discarded.

The lower viscosity portion was then centrifuged for a period of 30 minutes at a rate of 1100 rpm. There was a phase separation, whereupon the resulting lower viscosity phase was decanted off and the higher-viscosity phase was discarded. The resulting, lower viscosity phase was then treated in a volume ratio of 1 (decanted phase): 4 (water) with demineralized water. This mixture was heated to a temperature of 96 ° C over a period of 60 minutes. After cooling the mixture to ambient temperature, a phase separation occurred, after which the lower-viscosity portion was separated by decantation. The higher-viscosity portion was discarded. The resulting lower-viscosity portion was centrifuged at a rate of 1100 rpm for a period of 30 minutes, resulting in phase separation. The higher-viscosity phase was discarded. The decanted off, lower viscosity portion was held for a period of 24 hours in a cold room at a temperature of about 10 ° C, with a clear phase separation occurred. The resulting higher viscosity phase was discarded and the lower viscosity phase decanted off, the latter then being centrifuged for a period of 30 minutes at a rate of 1100 rpm.

The resulting lower viscosity phase was the intermediate product of the present invention, a readily mobile oil of yellowish color which could be stored stably without the addition of preservatives.

The resulting egg oil was boiled with distilled water for about one hour. It was then cooled to room temperature. Then, the water was distilled off and the distillation residue was added with ethanol. This mixture was allowed to stand for about one hour at a temperature of about 77 ° C. Subsequently, the ethanol was distilled off.

The distillate (alcoholic tincture) was finally mixed with the previously collected egg oil to ultimately obtain the new, highly effective egg oil composition.

Claims

claims

1. Verfaliren for the production of an egg oil composition from the yolk of birds or reptiles for the treatment of skin burns and skin diseases with the following process steps:

a) drying the egg yolk at temperatures up to 90 ° C and crushing to a powdery product (A);

b) extracting the product (A) from step (a) with a fat-dissolving extractant; c) distilling off the extractant to form a viscous residue (B);

d) aging the residue (B) from step (c) at ambient temperature for a period of up to 10 hours;

e) further aging of the residue (B) at a temperature of less than 20 ° C until phase separation occurs;

f) separating the lower viscosity phase (( ^" ) formed in the phase separation in step (e);

g) centrifuging the lower viscosity product (C) from step (f) at a rate of at least 900 revolutions per minute, and separating the lower viscosity phase (D);

h) mixing the product (D) from step (g) with demineralized water and heating the mixture to a temperature of at least 80 ° C:

t) cooling the aqueous mixture of step (h) to ambient temperature and separating the lower-viscosity part (E);

j) centrifuging the product (E) from step (i) at a rate of at least 900 revolutions per minute and separating the lower viscosity phase (F); k) maintaining the product (F) of step (j) at temperatures less than 20 ° C until phase separation occurs, and separating the lower viscosity phase (G);

1) centrifuging the product (G) from step (k) at a rate of at least 900 revolutions per minute and separating the lower viscosity phase (H) (= egg oil);

m) boiling the recovered egg oil with distilled water for a period of at least 20 minutes;

n) allow to cool to room temperature;

o) distilling off the water;

p) Allow the distillation residue to stand with ethanol and allow it to stand for at least one minute at a maximum temperature of 78 ° C.

q) distilling off the ethanol;

r) mixing the distillate (alcoholic tincture) with egg oil (phase H).

2. The method according to claim 1, characterized in that the measures of steps (h) to (1) or (j) to (1) with the product (H) of the stage (1) are repeated.

3. The method according to claim 1 or 2, characterized in that in the context of process step (d) the aging of the residue (B) from step (c) takes place at ambient temperature for a period of five to seven hours and in the context of process step (H) the mixture is heated to a temperature of preferably 95 to 100 ° C, preferably for a period of about 30 to 90 minutes, in particular of about 60 minutes.

4. The method according to any one of claims 1 to 3, characterized in that the egg yolk is dried at a temperature between 50 ° C and 70 ° C.

5. The method according to any one of claims 1 to 4, characterized in that the extracting agent used for the extraction of (A) in step (b) from the following substances, or substance groups aa) to ee) is selected: aa) aliphatic alcohols having one to five, preferably one to four carbon atoms in the molecule, preferably methanol or ethanol,

bb) aliphatic ketones having up to five carbon atoms in the molecule, preferably acetone,

cc) Freon,

dd) aliphatic ethers having up to seven carbon atoms in the molecule, in particular dialkyl ethers, such as diethyl ether, and cyclic ethers, in particular tetrahydrofuran, ee) C0 ₂ .

6. The method according to any one of claims 1 to 5, characterized in that the extraction technique used for the extraction of (A) in step (b) from the below-mentioned extraction techniques al) to dl) is selected: al) Maceration at pressures between vacuum and moderate overpressure, at room temperature or at elevated temperature, and / or

bl) Extraction according to the Soxleth method, and / or

cl) pressure extraction with C0 ₂ , and / or

dl) Mild boiling under reflux.

7. The method according to any one of claims 1 to 6, characterized in that the extraction of (A) in step (b) is carried out for three to seven, preferably four to five days long.

8. The method according to any one of claims 1 to 7, characterized in that either carried out as a separation process for separating the lower-viscosity phases (C) to (H), a decanter or a separator is used.

9. The method according to any one of claims 1 to 8, characterized in that the centrifuging of (C), (E) and (G) in the steps (g), (j) and (1) each during a period of about 20 to 40, preferably about 30 minutes is made.

10. Verfaliren according to one of claims 1 to 9, characterized in that the ethanol used in step (p) is medical alcohol.

1 1. A method according to any one of claims 1 to 10, characterized in that the ethanol used in step (p) in an amount of 10-50% by weight, based on the

Total amount of the distillation residue is used, wherein the mixture is preferably mixed at intervals.

12. The method according to any one of claims 1 to 1 1, characterized in that the Altera of the residue (B) in step (e) at a temperature between 7 ° C and 12 ° C, preferably during a period up to 24 hours.

13. The method according to any one of claims 1 to 12, characterized in that the holding of the product (F) from step (j) in step (k) at temperatures of about 7 to 12 ° C, preferably for a period up to 24 hours he follows.

14. Verfaliren according to one of claims 1 to 13, characterized in that the cooking in step (m) takes place for about 30 to 60 minutes.

15. A composition prepared according to the method of any one of claims 1 to 14 for the treatment of skin burns, sunburn, closed wounds (for example, torsions), open wounds, scars, skin diseases such as atopic dermatitis, psoriasis, acne, eczema, and as a means of regeneration in burns.