WO2014129365A1 - 溶解物濃度の測定方法 - Google Patents
溶解物濃度の測定方法 Download PDFInfo
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- WO2014129365A1 WO2014129365A1 PCT/JP2014/053244 JP2014053244W WO2014129365A1 WO 2014129365 A1 WO2014129365 A1 WO 2014129365A1 JP 2014053244 W JP2014053244 W JP 2014053244W WO 2014129365 A1 WO2014129365 A1 WO 2014129365A1
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- light
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- colorant
- region component
- concentration
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
Definitions
- the present invention relates to a lysate concentration measurement method for measuring the concentration of a lysate in a sample based on the absorbance of light.
- the concentration of the dissolved substance dissolved in the liquid is often measured using an absorptiometric method.
- absorptiometry for example, after a sample having a specific lysate is stored in a transparent measurement cell, a reagent is added to the sample, and the sample is colored according to the concentration of the specific lysate. Use as the measurement solution.
- the light to be measured is transmitted through the liquid to be measured, and a part of the light is absorbed by the liquid to be measured, and then the transmitted light is received by the light receiver. Measure the light intensity.
- the absorbance value is calculated from the intensity of the transmitted light measured at this time and the intensity of the transmitted light of a specific wavelength of light with respect to the transparent liquid, which is measured separately. Then, using this absorbance value and a calibration curve prepared in advance for this lysate, that is, a diagram showing the relationship between the absorbance value and the lysate concentration value, the concentration of the lysate in the sample is determined. calculate.
- light including the visible light region is transmitted through the liquid to be measured, and the light in the red region component, the light in the green region component, or the blue light obtained by dividing the light in the visible light region into approximately three parts of the transmitted light. It is also possible to measure the concentration of a specific lysate in a sample by calculating an absorbance value for any of the region component lights, or for a plurality of region component lights that are a combination thereof (patents). Reference 1).
- the lysate concentration is not detected at all even though there is a lysate to be detected in the sample, or it is less than the actual lysate concentration and only the value corresponding to the amount of reagent. Therefore, the concentration of the lysate is not detected, and there is a problem that the proper lysate concentration in the sample is not measured. This problem may also occur when measuring the concentration of the lysate using the light of three region components, red, green, and blue.
- the present invention uses the light absorbance of the three region components of red, green, and blue to measure the concentration of the lysate in the sample. It is an object of the present invention to provide a method for measuring a concentration of a lysate capable of determining whether or not it is added.
- light that includes a visible light region is transmitted through a liquid to be measured that is colored by the addition of a reagent to a sample, and the light in the visible light region of the transmitted light is approximately divided into three parts.
- the concentration of the lysate selected from the light of the red region component, the light of the green region component, the light of the blue region component, or the light of the plurality of region components obtained by combining these
- concentration of a specific lysate in the sample is measured by calculating the light absorbance of the region component for the lysate concentration measurement based on the transmitted light from the liquid to be measured to which is added Concentration measurement step and coloring Based on the transmitted light from the liquid to be measured to which an entering reagent is added, by calculating the absorbance
- the absorbance value of the three region component lights when the liquid to be measured that has developed color due to the dissolved material absorbs, for example, all three region component lights (hereinafter referred to as region component light), the absorbance value of the three region component lights.
- the region component light having a linear relationship between the concentration of the lysate and the lysate is selected as the region component light for lysate concentration measurement.
- the colorant may be any colorant as long as the liquid to be measured that has developed color transmits the region component light for measuring the dissolved substance concentration without absorbing it.
- this colorant one that causes the liquid to be measured to be colored in the same color as the region component light for measuring the dissolved substance concentration can be used.
- the liquid to be measured that has developed color due to the colorant passes through the region component light for measuring the concentration of lysate without absorbing it. Can be easily obtained from a calibration curve prepared in advance.
- the region component light for measuring the lysate concentration for example, the region component light other than the green region component light, that is, either the red region component light or the blue region component light, for example,
- the red region component light passes through the liquid to be measured, and thus shows the absorbance value A3 caused by the colorant alone or the absorbance value A1 caused by the colorant and the specific dissolved material. If the red region component light shows absorbance due to the colorant and the specific lysate, the concentration of the specific lysate is known, so the absorbance of the red region component light due to the specific lysate concentration alone
- the value A2 is also easily calculated from a calibration curve created in advance.
- the first colorant that develops the liquid to be measured into a color that allows transmission of the light of the region component for measuring the concentration of the dissolved substance and one of the light of the remaining region component without absorbing it.
- the color of the region component for measuring the lysate concentration and the color of the other component of the remaining region component are allowed to pass through without being absorbed.
- the second colorant for coloring the measurement solution is added to the other of the reagents to make a second colorant-containing reagent.
- the colorant that transmits two region component lights can cause the liquid to be measured to develop the same color as the complementary color light of the blue region component light. That's fine.
- the colorant that transmits the red region component light and the blue region component light without absorbing them may be any colorant that causes the liquid to be measured to develop the same color as the complementary color light of the green region component light.
- the colorant that transmits the component light and the blue region component light without absorbing it may be any colorant that causes the liquid to be measured to develop the same color as the complementary color light of the red region component light.
- the first colorant has, for example, the same liquid as the complementary color light of the green region component light.
- a color developing agent is used, and as the second colorant, one that causes the liquid to be measured to develop the same color as the complementary color light of the blue region component light is used. Since the red region component light is not absorbed by the liquid to be measured in which only the first colorant and the second colorant are colored, the concentration of the specific lysate in the sample is the absorbance of the red region component light. Using the value, it can be easily obtained from a calibration curve prepared in advance.
- the green region component light since the green region component light is not absorbed by the liquid to be measured only by the coloration of the second colorant, the green region component light shows the absorbance value B3 caused only by the first colorant, or the first coloration.
- the absorbance value B1 attributed to the agent and the specific lysate is shown.
- the blue region component light is not absorbed by the liquid to be measured that is only colored by the first colorant, and therefore shows the absorbance value C3 due to only the second colorant, or the second colorant And the absorbance value C1 due to the specific lysate.
- the liquid to be measured is colored so as to change color with a change in the concentration of the specific dissolved matter, and the light of the two region components is emitted.
- the color of the solution to be measured is changed to a color that allows the light of the two region components to pass through without being absorbed.
- an agent is added to the reagent to make a reagent with a colorant.
- the colorant when two region component lights for measuring the concentration of lysate are, for example, a blue region component light and a green region component light, the colorant contains other region component light, that is, red region component light. Those that color the liquid to be measured in the same color as the complementary color light are used. Since the two component light components for measuring the concentration of the lysate are not affected by the colorant in the liquid to be measured, the concentration of the specific lysate is calculated based on the absorbance value of the region component light. Further, the remaining red region component light shows an absorbance value attributed only to the colorant, or an absorbance value attributed to the colorant and the specific lysate.
- the absorbance value caused only by the colorant can be easily calculated. Therefore, the suitability of the added amount of the reagent added to the sample can be easily determined based on the absorbance value caused only by the colorant of the red region component light.
- the colorant-containing reagent is recycled. That is, the concentration measurement step and the determination step are repeated while adding.
- the necessary amount of the reagent has been added even after a predetermined time has elapsed. If it is determined that there is no, in the determination step, an alarm is issued and the measurement is stopped.
- a colorant that colors the liquid to be measured is added to the reagent in a color that transmits the light of the region component for measuring the concentration of the dissolved substance without absorbing it. Therefore, the concentration of a specific lysate in the sample can be measured by the light absorbance of the area component for measuring the lysate concentration, and whether the reagent is added in the required amount by the light absorbance of the other area components. It can be determined whether or not. Therefore, in the present invention, it is possible to always measure the correct concentration of the lysate without worrying about the amount of reagent added.
- FIG. 1 shows a concentration measuring apparatus for carrying out the present invention.
- the concentration measuring apparatus 1 automatically measures the concentration of a dissolved substance, such as dissolved oxygen, phosphoric acid, alkalinity component, hardness component, or silica, dissolved in industrial water or daily life water, using light absorbance. To do.
- a dissolved substance such as dissolved oxygen, phosphoric acid, alkalinity component, hardness component, or silica
- the concentration measuring device 1 is attached to one side surface of a measuring cell 2 in which a measuring solution S2 or a colorless adjusting solution S0 colored inside is stored, and the measuring cell 2 A light receiving / emitting unit 3 that performs light emission to the side and reception of transmitted light from the measurement cell 2, a sample supply line 4 that supplies the measurement cell 2 with a sample solution S1 or a preparation solution S0 having a specific lysate,
- the reagent T0 is added to the sample solution S1 in the measurement cell 2, and this sample solution S1 is used as the measured solution S2 colored by a specific lysate, and from the measurement cell 2 to the measured solution S2.
- a liquid discharge line 6 for discharging the adjustment liquid S0, an arithmetic processing device 7 for inputting and outputting to the light receiving and emitting unit 3, and a processing result by the arithmetic processing device 7 are output.
- the sample liquid S1 is colorless and transparent, and therefore is also used as the adjustment liquid S0.
- the measurement cell 2 has a box shape with a capacity of 2.5 mL, the left side surface portion is formed of a white reflection plate 21, and the central portion of the right side surface portion facing the reflection plate 21. In addition, an acrylic transparent portion 22 is formed.
- the measurement cell 2 has a front surface, a rear surface portion, an upper surface portion, and a lower surface portion that are formed of a black plate on the inner surface, a sample supply line 4 is connected to the lower surface portion, and a liquid discharge line 6 is connected to the upper surface portion. Are connected, and the reagent supply line 5 is connected to the left side surface portion.
- the light emitting / receiving unit 3 includes a light emitting body 31, a light receiving body 32, a wiring base (not shown), and the like in a casing provided with an opening on the measurement cell 2 side.
- the illuminator 31 emits light into the measurement cell 2 and transmits this light into the measured liquid S2 or the adjustment liquid S0.
- a light source such as a light emitting diode (LED) that emits light (white light) including a visible light region is used.
- the light receiving body 32 receives the transmitted light L of the light emitted from the light emitting body 31 from the measured liquid S2 or the adjustment liquid S0, and measures the intensity of the light related to the transmitted light L.
- the photoreceptor 32 includes three photodiodes, light of a red region component (hereinafter referred to as red region component light), and light of a green region component (hereinafter referred to as “red region component light”).
- red region component light three photodiodes, light of a red region component (hereinafter referred to as red region component light), and light of a green region component (hereinafter referred to as “red region component light”).
- Three color filters F that transmit only the light of the blue region component (hereinafter referred to as blue region component light), that is, the R filter, the G filter, and the B filter, respectively.
- an RGB color sensor having a photodiode D1 having an R filter, a photodiode D2 having a G filter, and a photodiode D3 having a B filter is used for the photoreceptor 32 (See FIG. 2).
- the light receiving body 32 includes red region component light, green region component light, and blue region component light (hereinafter referred to as three region component lights) transmitted through each filter, of the transmitted light L transmitted through the measurement liquid S2. Simultaneously measure the light intensity.
- the R filter transmits the red light most of the red region component light
- the G filter transmits the green light most of the green region component light
- the B filter transmits the blue light most of the blue region component light.
- the light receiving body 32 is disposed on the same side as the light emitting body 31 with respect to the measurement cell 2. Further, the transmitted light L in the measured liquid S2 emitted from the light emitter 31 is reflected by the reflecting plate 21 facing the light emitter 31 with the measured liquid S2 interposed therebetween, and is transmitted again through the measured liquid S2. . Therefore, the light receiving body 32 receives the transmitted light L reflected by the reflecting plate 21.
- the light emitter 31 and the light receiver 32 are such that the intersection point P of the light emitter 31 with the optical axis K1 of the reflector 21 and the intersection of the light receiver 32 with the optical axis K2 of the reflector 21 substantially coincide. It is positioned. Therefore, the main light from the light emitter 31 that is reflected by the reflector 21 does not reach the light receiver 32, and the light receiver 32 is a part of the reflected light from the ambient light around the main light from the light emitter 31. Or a part of the light irregularly reflected by the reflecting plate 21 or both of them.
- FIG. 2 shows a circuit diagram in the light emitting / receiving unit 3.
- reference sign D1 is a photodiode having an R filter
- reference sign D2 is a photodiode having a G filter
- reference sign D3 is a photodiode having a B filter, and these are integrated.
- the photoreceptor 32 is formed.
- symbol H denotes a light emitting diode (LED) that becomes the light emitter 31
- symbols C1, C2, and C3 denote main circuits for the photodiodes D1, D2, and D3, and symbols O1, O2, and so on.
- O3 is an operational amplifier (operational amplifier) for each of the photodiodes D1, D2, and D3.
- the signal of the transmitted light intensity of each region component light output from the light receiving body 32 is transmitted to the arithmetic processing unit 7 through the operational amplifiers O1, O2, and O3.
- the sample supply line 4 includes a sample pump 41, a membrane filter 42, an electromagnetic valve 43, a main pipe 44, and a return pipe 45, and is sampled at a predetermined place.
- the sample liquid S1 is supplied to the measurement cell 2.
- the sample pump 41 is always operating, and continues to supply the sample solution S1 to the measurement cell 2 side via the main pipe 44.
- the electromagnetic valve 43 is closed, and the entire amount of the sample liquid S1 before being filtered by the membrane filter 42 is discharged to the return pipe 45 side.
- the electromagnetic valve 43 is opened, the sample liquid S1 is filtered by the membrane filter 42, supplied to the measurement cell 2, and then discharged from the liquid discharge line 6 side.
- the reagent supply line 5 includes a reagent pump 51, a reagent bottle 52, and a pipe 53.
- the reagent pump 51 By operating the reagent pump 51 for a predetermined time, the reagent T0 in the reagent bottle 52 is measured by a predetermined amount. Supply to cell 2.
- the sample liquid S1 and the reagent T0 are sufficiently stirred in the measurement cell 2. That is, in the measurement cell 2, the liquid to be measured S2 colored according to the concentration of a predetermined lysate is produced after a certain time by adding the reagent T0.
- the liquid discharge line 6 discharges the measured liquid S2 or the adjustment liquid S0 (hereinafter referred to as waste liquid S3), which has been measured, in the measurement cell 2 to the outside of the measurement cell 2.
- the waste liquid S3 is discharged from the measurement cell 2 by supplying the sample liquid S1 or the adjustment liquid S0 from the sample supply line 4 to the measurement cell 2 for a certain period of time.
- the calculation processing device 7 is a computer that operates according to a program, and includes a calculation unit 71 and a storage unit 72.
- the calculation unit 71 calculates, for example, the time average intensity for each region component light based on the intensity signals of the three region component lights output from the photoreceptor 32. In addition, the calculation unit 71 calculates, for example, the absorbance of the three region component lights using the transmitted light intensity of partially absorbed light and the transmitted light intensity of non-absorbed light, and the three region components. The concentration of the lysate to be measured is calculated from each absorbance value for light. Further, for example, the calculation unit 71 receives a preparation completion signal from the control device 9 and causes the light emitter 31 to emit light in a timely manner, and also causes the control device 9 to start measurement, end measurement, and stop measurement. It also has a function to send the signal.
- the storage unit 72 stores a calibration curve indicating the relationship between the absorbance and the lysate concentration for the necessary region component light. Note that another function as described later is also added to the arithmetic unit 71 and the like.
- the output device 8 displays on the display the concentration of the specific dissolved matter in the sample liquid S1 calculated by the arithmetic processing device 7.
- the control device 9 controls the opening and closing of the electromagnetic valve 43 of the sample supply line 4 to discharge the waste liquid S3 in the measurement cell 2 by the sample liquid S1 and store the sample liquid S1 in the measurement cell 2. Has a function. Further, the control device 9 performs ON / OFF control of the reagent pump 51 of the reagent supply line 5 to supply a predetermined amount of the reagent T0 to the sample solution S1 in the measurement cell 2, and this sample solution S1 is used as the solution to be measured. It has the function of changing to S2.
- control device 9 receives the measurement end signal from the arithmetic processing device 7, discharges the waste liquid S3 in the measurement cell 2, supplies the reagent T0 to the measurement cell 2, and after a predetermined time has elapsed, That is, after the solution to be measured S2 sufficiently colored by the reagent T0 is made, a signal indicating completion of preparation is transmitted to the arithmetic processing unit 7.
- the sample pump 41 is operated with the electromagnetic valve 43 of the sample supply line 4 closed.
- the sample solution S1 supplied from the main pipe 44 to the measurement cell 2 side is discharged only from the return pipe 45 side without being filtered.
- the electromagnetic valve 43 is opened, and the filtered sample liquid S1 is discharged from the liquid discharge line 6 side through the measurement cell 2.
- the electromagnetic valve 43 is closed, the sample solution S1 is discharged only from the return pipe 45 side, and a predetermined amount of the sample solution S1 is stored in the measurement cell 2.
- the liquid to be measured S2 is colored.
- the measured liquid S2 is sufficiently colored, light including a visible light region is emitted from the light emitter 31 of the light receiving and emitting unit 3. This light is transmitted through the measured liquid S2 in the measurement cell 2, then reflected by the reflecting plate 21, and again transmitted through the measured liquid S2.
- the transmitted light L is received by the light receiving body 32 after a part of the light is absorbed by the liquid S2 to be measured. In this case, the transmitted light L is divided into three region component lights, and the intensity of each transmitted light is measured. Then, the emission of light from the light emitter 31 is repeated a plurality of times, and the average transmitted light intensity of the three region component lights is calculated.
- the electromagnetic valve 43 of the sample supply line 4 is opened, and the filtered sample liquid S1 is poured into the measurement cell 2 for a predetermined time. Accordingly, the waste liquid S3 in the measurement cell 2 is discharged through the liquid discharge line 6, the inside of the measurement cell 2 is cleaned, and the sample liquid S1 that becomes the adjustment liquid S0 is stored in the measurement cell 2. . Subsequently, with respect to the transmitted light from the adjustment liquid S0, the average transmitted light intensity of the three region component lights is calculated as in the case of the measured liquid S2.
- the respective absorbances for the three region component lights are calculated.
- the region component light created for the region component light for measuring the concentration of a specific lysate (hereinafter referred to as the region component light for measuring the lysate concentration) From the calibration curve indicating the relationship between the absorbance and the concentration of the lysate, the concentration of the specific lysate at that time is calculated. Further, the value of the concentration of the specific lysate is displayed on the display of the output device 8.
- FIG. 3 (a) shows a case where the specific lysate in the sample solution S1 is Wellclin (registered trademark of Kurita Kogyo Co., Ltd., which means a dithiocarbamic acid heavy metal scavenger).
- the values of the absorbances of the light components of one region are shown for each wellcrine concentration.
- FIG. 3 (b) is a graph showing a calibration curve. Show. Among the calibration curves shown in FIG. 3 (b), the absorbance value for the blue region component light has the most linear change in the absorbance value with respect to the wellclin concentration. Therefore, it can be seen that it is preferable to determine the wellclin concentration based on the absorbance of the blue region component light as the region component light for measuring the concentration of the lysate.
- a ferrous chloride solution 250 mg / L is used as the reagent T0 that causes the sample solution S1 containing wellclin to develop a brown color to be measured solution S2. Further, since the liquid S2 to be measured absorbs a lot of light in the visible light, it absorbs any of the three region component lights.
- the concentration measuring apparatus 1 the light from the light emitter 31 is transmitted so as to reciprocate obliquely through the measured liquid S2 in the measurement cell 2, and the light passing distance in the measured liquid S2 becomes longer. In addition, the amount of light absorbed into the liquid S2 to be measured can be increased accordingly. Therefore, the concentration measuring apparatus 1 can measure the dissolved substance concentration with high accuracy and can downsize the measuring cell 2.
- the concentration measuring apparatus 1 the light including the visible light region from the light emitter 31 is divided into three region component lights by the light receiver 32, and the absorbance of these region component lights is calculated, thereby dissolving the region component light.
- the concentration of the object is determined. Therefore, in this concentration measuring apparatus 1, in order to measure the concentration of any lysate, it is only necessary to have a simple set of the light emitter 31 and the light receiver 32, thereby reducing the measurement cost and reducing the size of the measurement apparatus. Can be achieved.
- the absorbance is calculated for all three region component lights.
- this absorbance may be calculated only for the region component light for measuring the lysate concentration.
- the liquid to be measured S2 is colored yellow-orange due to the addition of the reagent T0, the liquid to be measured S2 hardly absorbs red region component light and green region component light, and is blue light that is complementary color light. It is considered that only region component light is absorbed. Therefore, in this case, the blue region component light becomes the region component light for measuring the dissolved substance concentration, and the absorbance may be calculated only for the blue region component light.
- the reagent T0 is definitely added to the sample solution S1 when the lysate concentration is measured as 0 (zero).
- the measured concentration value of the lysate is small, there is a question as to whether the reagent T0 is sufficiently added to the sample solution S1.
- Such non-addition or insufficient addition of the reagent may occur due to clogging or disconnection of the pipe 53 in the reagent supply line 5, failure of the reagent pump 51, or depletion of the reagent T 0 in the reagent bottle 52. is there. Therefore, in measuring the lysate concentration, it is important to be able to determine whether or not the necessary amount of the reagent T0 is added to the sample solution S1.
- This lysate concentration measuring method is based on a reagent preparation step of adding a colorant to the reagent T0 to produce a colorant-containing reagent T0c, and a transmitted light L from the measured liquid S2 to which the colorant-containing reagent T0c is added. Based on the concentration measurement step for measuring the concentration of the specific lysate in the sample liquid S1 and the transmitted light L from the measured liquid S2 to which the colorant-containing reagent T0c is added, only the necessary amount of the reagent T0 is obtained. And a determination step of determining whether or not it is added.
- a colorant that develops the liquid S2 to be measured is added to the reagent T0 in a color that allows the region component light for measuring the concentration of the dissolved substance of the three region component lights to pass through without being absorbed.
- a colorant-containing reagent T0c is produced.
- This reagent preparation process will be described in detail with reference to FIG.
- region component light for lysate concentration measurement As a premise of this reagent preparation process, it is necessary to determine region component light for lysate concentration measurement from three region component lights.
- As the area component light for measuring the dissolved substance concentration one of the three area component lights that is absorbed by the measured liquid S2 colored by a specific dissolved substance is selected.
- the absorbance value and the lysate concentration value change in a linear relationship.
- the region component light to be selected is selected as the region component light for measuring the dissolved substance concentration.
- a colorant that develops the liquid S2 to be measured is selected as a color that transmits the region component light for measuring the concentration of the dissolved matter without absorbing it (step S11).
- FIG. 5 shows a hue circle in which the twelve colors that visible light may show are arranged in the order in which the colors change.
- the hue ring is divided into three parts, and red region component light is indicated by, for example, red light, red orange light, yellow orange light, and yellow light, and green region component light is indicated by, for example, yellow green light,
- red region component light is indicated by, for example, red light, red orange light, yellow orange light, and yellow light
- green region component light is indicated by, for example, yellow green light
- green light, blue-green light, and green-blue light are indicated
- blue region component light is indicated by, for example, blue light, blue-violet light, purple light, and red-violet light.
- the red region component light is absorbed by the measured liquid S2 that develops the same color as the complementary color light (either blue-green, green-blue, blue, blue-violet, or a mixed color of these colors). Further, the green region component light is absorbed by the measured liquid S2 that develops the same color (purple, magenta, red, red-orange, or a mixed color thereof) as the complementary color light, and the blue region. The component light is absorbed by the measured liquid S2 that develops the same color as the complementary color light (yellow-orange, yellow, yellow-green, or green, or a mixed color of these colors).
- the red region component light is not absorbed in the measured liquid S2 that develops the same color (either red, red orange, yellow orange, or yellow, or a mixed color of these colors).
- the green region component light is not absorbed in the measured liquid S2 that develops the same color (yellowish green, green, blue-green, or green-blue, or a mixed color of these colors).
- the blue region component light is transmitted without being absorbed in the measured liquid S2 that develops the same color (blue, bluish purple, purple, reddish purple, or a mixture of these colors). To do.
- the area component light for measuring the dissolved substance concentration is the red area component light
- a colorant that develops the color of the liquid S2 to be measured in the same color as the red area component light may be used.
- the region component light for measuring the dissolved substance concentration is the green region component light
- the colorant may be one that colors the liquid S2 to be measured in the same color as the green region component light.
- the concentration measurement region component light is blue region component light
- a colorant that develops the liquid S2 to be measured in the same color as the blue region component light may be used.
- the colorant is colored in the same color (either red, red orange, yellow orange, or yellow) as one color light in the red region component light
- this colorant is added.
- the liquid S2 to be measured absorbs either the green region component light or the blue region component light other than the red region component light, but the colorant has the same color (red, In the case of color development (a mixed color of red-orange, yellow-orange, and yellow), the liquid S2 to which the colorant is added absorbs both green region component light and blue region component light.
- a predetermined amount of this colorant is added to a predetermined amount of reagent T0 to produce a colorant-containing reagent T0c (step S12).
- the concentration measurement process is substantially the same as the series of work steps described for the concentration measurement apparatus 1. That is, in the concentration measurement step, the sample liquid S1 is supplied to the measurement cell 2, and then the reagent T0c containing the colorant is added to the sample liquid S1 to make the measurement liquid S2, and the light emitter 31 is added to the measurement liquid S2. A step of transmitting the light from the light-emitting body 31, and a step of receiving the transmitted light L by the light receiving body 32, and supplying the adjustment liquid S0 to the measurement cell 2, and then transmitting the light from the light emitter 31 to the adjustment liquid S0.
- the absorbance of the region component light for measuring the lysate concentration is calculated from the step of receiving the transmitted light L by the light receiving body 32 and the transmitted light L of the liquid to be measured S2 and the adjustment liquid S0.
- the absorbance of the region component light for measuring the lysate concentration is calculated from the transmitted light L of the new measured solution S2 and the adjustment solution S0, and the specific lysate concentration in the sample solution S1 is measured from this absorbance. And a process of performing. Since the region component light for measuring the lysate concentration is not affected by the colorant, an appropriate lysate concentration is measured by this concentration measuring step except for the problem of the addition amount of the reagent T0.
- the determination step includes step 1, step 2, step 3, and step 4.
- step 1 in the concentration measurement step, when calculating the absorbance of the region component light for measuring the dissolved substance concentration from the transmitted light L of the liquid to be measured S2 and the adjustment liquid S0, The absorbance for the region component light is calculated.
- the absorbance for the other region component light is caused by only the colorant, the value is A3, and when this is caused by the colorant and the specific dissolved matter, the value is A1.
- the absorbance value A3 corresponds to the amount of reagent T0 added to the sample solution S1.
- step 3 the light absorption value A3 of the other region component light obtained in step 1 or step 2 due to only the colorant and the other region when the necessary amount of reagent T0 is added to the sample liquid S1.
- the component light is compared with the value A0 of the absorbance due to only the colorant (hereinafter referred to as the standard absorbance). If A3 ⁇ A0, it is determined that the required amount of reagent T0 is not added to the sample solution S1, and if A3 ⁇ A0, it is determined that the required amount of reagent T0 is added to the sample solution S1. To do.
- step 4 when it is determined that the necessary amount of the reagent T0 is not added to the sample solution S1, the concentration measurement is immediately stopped or the concentration measurement is stopped after continuing under certain conditions.
- FIG. 4B shows the operation of the calculation unit 71 of the calculation processing device 7.
- the absorbance of the region component light for measuring the concentration of the lysate for example, the green region component light is calculated.
- the concentration of the specific lysate in the sample solution S1 is measured (step S22). Since the area component light for measuring the concentration of lysate (green area component light) is not affected by the colorant, the absorbance value and the area component light for measuring the concentration of lysate (green area component light) are prepared in advance.
- the specific lysate concentration in the sample solution S1 is easily calculated from the calibration curve showing the relationship between the lysate concentration and the absorbance.
- the absorbance value of other region component light (red region component light) at this time is defined as A1.
- step S24 The absorbance value A3 obtained in step S23 and the absorbance due to only the colorant of other region component light (red region component light) when the necessary amount of reagent T0 is added to the sample solution S1 (reference) Is compared with the value A0 (step S24).
- A3 ⁇ A0 it is determined YES and it is determined that the necessary amount of reagent T0 is added. If A3 ⁇ A0, NO is determined and the amount of reagent T0 added is insufficient. It is determined that
- step S24 If it is determined in step S24 that the necessary amount of reagent T0 has been added, the measurement of the concentration relating to the sample solution S1 is completed, and the measurement of the concentration of the new sample solution S1 is started (step S25). . That is, the control device 9 is instructed to discharge the adjustment liquid S0 in the measurement cell 2, that is, the waste liquid S3, and a new sample liquid S1 is stored in the measurement cell 2. The measurement operation is performed.
- the measurement may be stopped as it is without performing the remeasurement.
- the standard elapsed time from the end of the first measurement to the end of the third measurement is measured, and it is determined in the first measurement that the addition amount of the reagent T0 is insufficient, the standard If the target elapsed time elapses, the measurement may be stopped.
- the area component light for measuring the dissolved substance concentration is, for example, red area component light
- the colorant causes the liquid S2 to be colored to develop the same color as almost all the color light in the red area component light.
- the sum (B1 + C1) of the absorbance values of the other region component lights (green region component light and blue region component light) is calculated, and the absorbance values of these region component lights due to only the colorant are calculated.
- the sum (B3 + C3) is calculated from the equation (B1 + C1) ⁇ (B2 + C2). Since the sum of the absorbance values (B3 + C3) is a value corresponding to the amount of the reagent T0 in the measured solution S2, this value is the colorant when the necessary amount of the reagent T0 is added to the sample solution S1.
- this lysate concentration measurement method a colorant that develops the liquid S2 to be measured is added to the reagent T0 in a color that transmits the region component light for lysate concentration measurement without absorbing it. Based on the transmitted light of the liquid S2 to be measured to which the colorant-containing reagent T0c is added and the colorant-containing reagent T0c is added, the absorbance of the region component light for measuring the lysate concentration and the coloration of the other region component light Absorbance due to the agent alone is calculated. Therefore, in this lysate concentration measurement method, it is possible to easily determine whether or not the necessary amount of the reagent T0 is added to the sample solution S1 when measuring the lysate concentration. That is, in this method for measuring the lysate concentration, an appropriate lysate concentration can always be measured without worrying about the amount of reagent T0 added.
- the calculation unit 71 of the calculation processing device 7 has a function of causing the operation described in the flowchart of FIG.
- the calculation unit 71 has a function of calculating the value of the absorbance caused only by the colorant using the absorbance of the region component light other than the region component light for measuring the lysate concentration.
- the calculation unit 71 compares the calculated absorbance value with the reference absorbance value with respect to the absorbance caused only by the colorant, and when the calculated absorbance value is small or 0 (zero). In this case, it has a function of determining that the reagent T0 is not properly added.
- the calculation unit 71 has a function of causing the output device 8 to issue an alarm to that effect and causing the control device 9 to stop the measurement.
- the storage unit 72 of the arithmetic processing unit 7 stores a reference absorbance value attributable to only the colorant of the region component light other than the region component light for measuring the dissolved substance concentration. Further, the storage unit 72 stores a calibration curve indicating the relationship between the absorbance and the lysate concentration for the region component light other than the lysate concentration measurement region component light.
- blue region component light is used as the region component light for measuring the lysate concentration
- ferrous chloride solution 250 mg / L
- the colorant includes a dye solution (50 mg / L) that causes the liquid S2 to be measured to develop the same color as the all-color light in the blue region component light (a mixed color of blue, blue purple, purple, and red purple). Used. Therefore, the colorant-containing reagent T0c is prepared by adding a certain amount of a dye solution to a certain amount of ferrous chloride solution.
- the capacity of the measurement cell 2 is 2.5 mL, when a predetermined amount of the reagent T0c containing the colorant is added thereto, the sample liquid S1 slightly overflows, and the liquid to be measured in the measurement cell 2 Only 2.5 mL of S2 is made.
- (A) of FIG. 6 adds 0.2 mL of reagent T0c with a colorant to each of six types of sample liquids S1 having different wellclin concentrations in the measurement cell 2, and the absorbance of the liquid S2 to be measured is Three region component lights are examined.
- the absorbance values A and B of the red region component light and the green region component light are affected by the wellclin and the colorant, and the values are shown in FIG. 3 (a).
- the absorbance value C of the blue region component light is not affected by the colorant, the value is almost the same as that shown in FIG. ing. Therefore, it can be seen that the concentration of wellclin can be calculated from the absorbance of the blue region component light even when the colorant-containing reagent T0c is used.
- the value D caused by wellclin and the colorant is the absorbance value of the red region component light.
- the sum F of absorbance values attributed only to this colorant corresponds to the amount of reagent T0 added. Is the reagent T0 added to the sample liquid S1 by a necessary amount by the sum of absorbance values F? A determination of whether or not can be made.
- FIG. 6 (b) shows the case where only 0.1 mL of the colorant-containing reagent T0c is added to each sample solution S1 having a different wellclin concentration in the measurement cell 2, and FIG. A case where only 0.05 mL of the colorant-containing reagent T0c is added to each sample solution S1 is shown.
- the sum F of the absorbance values attributable to the colorant alone in the red region component light and the green region component light is 0. This is 1 ⁇ 2 of the value in the case of 2 mL, and twice the value in the case where the amount of the colorant-containing reagent T0c is 0.05 mL. From this, it can be seen that the sum F of absorbance values attributed only to this colorant is a value proportional to the amount of the colorant-containing reagent T0c added.
- the amount of the colorant-containing reagent T0c is 0.05 mL
- the absorbance value of each region component light becomes constant and becomes smaller than the actual value. It can be seen that the amount of the reagent T0 added to the sample solution S1 is insufficient. Therefore, when the concentration of wellclin in the sample liquid S1 can be expected to be, for example, about 100 mg / L, the amount of the colorant-containing reagent T0c is set to 0.1 mL or more, and the red region component light and the blue region component light
- the standard absorbance value relating to the sum F of absorbance values caused only by the colorant needs to be 0.42 or more, for example.
- FIG. 7 shows three cases where a plurality of liquids to be measured S2 are prepared by adding 0.05 mL of the colorant-containing reagent T0c to the sample liquid S1 having a wellclin concentration of 0 mg / L in the measurement cell 2.
- the values of the absorbance of the region component light due to only the colorant and the sum of the values of the absorbance of the red region component light and the green region component light due to only the colorant are shown.
- FIG. 8 is a graph showing the relationship between the sum of the absorbance values attributable to only the colorant and the amount of addition of the colorant-containing reagent T0c for the red region component light and the green region component light. From the graph of FIG.
- the sum of the absorbance values due to only the colorant of the red region component light and the green region component light with respect to the addition amount of the colorant-containing reagent T0c is known. From the sum, it is possible to easily determine the standard absorbance value when the necessary amount of the reagent T0 is added.
- the first colorant-containing reagent T1c and the second colorant-containing reagent T2c may be made in the reagent preparation step for producing the colorant-containing reagent.
- the first colorant-containing reagent T1c is made by adding a certain amount of the first colorant to the predetermined amount of the first reagent T1
- the second colorant-containing reagent T2c is a certain amount of the second colorant. Only in addition to a predetermined amount of the second reagent T2.
- the first colorant includes region component light for measuring the concentration of dissolved matter, for example, red region component light and the remaining region component light, that is, green region component light or blue region component.
- One that develops the liquid S2 to be measured is used as a color that transmits one of the light, for example, green region component light without absorbing it.
- the second colorant has a color that transmits the dissolved component concentration measurement region component light (red region component light) and the other region component light (blue region component light) without absorbing it.
- a liquid that colors the liquid S2 to be measured is used.
- the colorant that develops the liquid S2 to be measured in a color that transmits the two region component lights without absorbing them will be described in detail using the hue ring of FIG.
- the colorant when the two region component lights are the green region component light and the blue region component light, the colorant has the same color as the complementary color light of the red region component light (blue green, green blue, blue, blue violet, or these Any color can be used as long as it develops in a mixed color).
- the colorant has the same color as the complementary color light of the green region component light (purple, magenta, red, or red-orange, or If the two region component lights are red region component light and green region component light, the complementary color light (yellow orange, yellow, yellow green, Or any one of green or a mixed color of these colors).
- the region component light for measuring the lysate concentration is red region component light
- a colorant that causes the liquid S2 to be measured to develop the same color as the complementary color light of the green region component light is used as the first reagent T1.
- the first colorant-containing reagent T1c is prepared, and a colorant that causes the liquid S2 to be measured to develop the same color as the complementary color light of the blue region component light is added to the second reagent T2 to produce the second color. What is necessary is just to make reagent T2c with an agent.
- the region component light (red region component light) for measuring the lysate concentration is not affected by the first colorant and the second colorant.
- the concentration of the lysate can be determined.
- the green region component light is affected only by the first colorant and is not affected by the second colorant. Therefore, for example, it is calculated using the calibration curve from the absorbance value B1 of the green region component light indicating the absorbance value due to the first colorant and the specific lysate, and the concentration value of the lysate.
- the absorbance value B3 caused only by the first colorant is calculated from the absorbance value B2 of the green region component light caused only by the lysate, and this absorbance value B3 is obtained from the reference absorbance value B0. If it is smaller, the added amount of the first reagent T1 is insufficient.
- the blue region component light is affected only by the second colorant and is not affected by the first colorant. Therefore, for example, it is calculated using the calibration curve from the absorbance value C1 of the blue region component light indicating the absorbance value due to the second colorant and the specific lysate and the concentration value of the lysate.
- the absorbance value C3 due to the second colorant alone is calculated from the absorbance value C2 of the blue region component light attributable only to the lysate, and this absorbance value C3 is obtained from the reference absorbance value C0. If it is smaller, the added amount of the second reagent T2 is insufficient.
- the measured liquid S2 in the measurement cell 2 is insufficient.
- Re-measurement may be performed by adding only the colorant-containing reagent.
- the calculation unit 71 of the calculation processing device 7 has a function of advancing the operation of the device as described above. Further, the storage unit 72 of the arithmetic processing unit 7 stores the reference absorbance values B0 and C0 of the respective component light components caused by only the colorant for the first reagent T1 and the second reagent T2. Yes.
- the first reagent T1 is added to the measurement cell 2 from the first reagent supply line 5A
- the second reagent T2 is supplied from the second reagent supply line 5B. It is added to the measuring cell 2.
- the first colorant-containing reagent T1c is also added to the measurement cell 2 from the first reagent supply line 5A
- the second colorant-containing reagent T2c is also added to the measurement cell 2 from the second reagent supply line 5B.
- the reagent supply lines 5A and 5B are provided with reagent pumps 51A and 51B, reagent bottles 52A and 52B, and pipes 53A and 53B, respectively. Controlled by
- FIG. 10 shows a case where the emission color of the solution S2 to be measured changes from the color of the complementary color light of the red region component light to the color of the complementary color light of the green region component light as the concentration of the dissolved matter increases.
- the absorbance value of the green region component light rapidly increases in a curved line when the concentration of the lysate begins to increase, and increases linearly when the concentration of the lysate exceeds 30.
- the absorbance value of the red region component light decreases linearly when the concentration of the lysate begins to increase, and gradually decreases when the concentration of the lysate becomes 30 or less. Therefore, the concentration of the lysate is calculated based on the absorbance value of the red region component light from 0 to 30, and if it exceeds 30, the concentration of the lysate is calculated based on the absorbance value of the green region component light. .
- the colorant added to the reagent T0 causes the liquid S2 to be measured to be colored in the same color as the complementary color light of the blue region component light, and is transmitted without absorbing the red region component light and the green region component light. What is to be used is used. Therefore, when the blue region component light shows, for example, the absorbance due to the colorant and the specific dissolved material, the absorbance value of the blue region component light due to only the colorant is calculated, and this If the absorbance value is smaller than the standard absorbance value, it is determined that the amount of reagent T0 added to the sample solution S1 is insufficient.
- a colorant-containing reagent T0c may be prepared by adding a colorant that causes the liquid S2 to be measured to develop the same color as the complementary color light of the remaining region component light in addition to the reagent T0. Then, from the absorbance value of the remaining region component light, the absorbance value attributed only to the colorant is calculated, and based on this absorbance value, whether or not the reagent T0 has been added to the sample solution S1 without a shortage. What is necessary is just to determine.
- the arithmetic unit 71 of the arithmetic processing unit 7 has a function of advancing the operation of the device as described above.
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Abstract
Description
この吸光光度法では、例えば、特定の溶解物を有する試料を透明な測定セル中に収容した後、この試料に試薬を添加して、この試料を特定の溶解物の濃度に応じて発色した被測定液とする。つぎに、この被測定液に、発光体から発せられた光を透過させて、この光の一部を被測定液に吸収させた後、この透過光を受光体で受光し、このときの透過光の強度を計測する。つぎに、このとき測定した透過光の強度と、別に測定した、例えば透明液に対する特定波長の光の透過光の強度とから、吸光度の値を算出する。そして、この吸光度の値と、この溶解物に関して予め作成した検量線、すなわち、吸光度の値と溶解物の濃度の値との関係を示す線図とを用いて、試料中の溶解物の濃度を算出する。
[濃度測定装置]
図1はこの発明を実施するための濃度測定装置を示している。
濃度測定装置1は、例えば、工業用水又は生活用水中等に溶解する、溶存酸素、リン酸、アルカリ度成分、硬度成分、又はシリカといった溶解物の濃度を、光の吸光度を用いて自動的に測定するものである。
まず、試料供給ライン4の電磁弁43を閉じた状態で、試料ポンプ41を作動させる。このことにより、主配管44から測定セル2側に供給される試料液S1は、濾過されずに、リターン配管45側のみから排出される。所定時間経過後、電磁弁43を開け、濾過された試料液S1を、測定セル2を通って、液排出ライン6側から排出させる。つづいて、所定時間経過後、電磁弁43を閉じ、試料液S1を、リターン配管45側からのみ排出させて、測定セル2内に、所定量の試料液S1を溜める。
つぎに、試薬T0が必要量だけ添加されているか否かの判定が可能な、この発明の一実施の形態に係る溶解物濃度の測定方法について、図4及び図5を参照しつつ説明する。
試薬調製工程では、3つの領域成分光のうちの、溶解物濃度測定用の領域成分光を、吸収することなく透過させる色に、被測定液S2を発色させる着色剤を、試薬T0に加えて着色剤入り試薬T0cが作られる。
この試薬調製工程の前提として、3つの領域成分光から、溶解物濃度測定用の領域成分光が定められている必要がある。溶解物濃度測定用の領域成分光には、3つの領域成分光のうち、特定の溶解物により発色した被測定液S2に吸収されるものが選定される。なお、3つの領域成分光のいずれもが、特定の溶解物により発色した被測定液S2に吸収される場合には、例えば、吸光度の値と溶解物濃度の値とが直線的な関係で変化する領域成分光が、溶解物濃度測定用の領域成分光として選定される。
濃度測定工程は、濃度測定装置1について説明した、一連の作業工程とほぼ同一である。すなわち、濃度測定工程は、試料液S1を測定セル2に供給した後、この試料液S1に着色剤入り試薬T0cを添加して被測定液S2を作る工程と、被測定液S2に発光体31からの光を透過させて、この透過光Lを受光体32で受光させる工程と、調整液S0を測定セル2に供給した後、この調整液S0に発光体31からの光を透過させて、この透過光Lを受光体32で受光させる工程と、被測定液S2と調整液S0との透過光Lから、溶解物濃度測定用の領域成分光についての吸光度を算出し、この吸光度から、試料液S1中の特定の溶解物の濃度を測定する工程と、試料液S1に必要量の試薬Tが添加されていない場合、測定セル2中の被測定液S2に、不足している着色剤入り試薬T0cを再添加して、新たな被測定液S2を作り、この新たな被測定液S2と調整液S0との透過光Lから、溶解物濃度測定用の領域成分光についての吸光度を算出し、この吸光度から、試料液S1中の特定の溶解物濃度を測定する工程とを有している。なお、溶解物濃度測定用の領域成分光は、着色剤の影響を受けないので、試薬T0の添加量の問題を除けば、この濃度測定工程により、適正な溶解物濃度が測定される。
判定工程は、工程1と、工程2と、工程3と、工程4とを有している。
工程1では、濃度測定工程において、被測定液S2と調整液S0との透過光Lから、溶解物濃度測定用の領域成分光についての吸光度を算出する際に、着色剤の影響を受ける他の領域成分光についての吸光度を算出する。そして、この他の領域成分光についての吸光度が、着色剤のみに起因する場合には、その値をA3とし、これが着色剤と特定の溶解物とに起因する場合には、その値をA1とする。なお、吸光度の値A3は、試料液S1に対する試薬T0の添加量に相当するものである。
つぎに、溶解物がウエルクリンである場合の、溶解物濃度の求め方と、試薬T0が必要量だけ添加されているか否かの判定方法について、図6~図8を参照しつつ具体的に説明する。
つぎに、試料液S1に、第1の試薬T1と第2の試薬T2とが添加されて、被測定液S2が作られる場合について説明する。なお、試薬が2種類の場合の濃度測定装置1Aは、図9で示されている。
つぎに、被測定液S2が、特定の溶解物の濃度に従って、第1色から第2色に変色していく場合について、図10を参照しつつ説明する。なお、この場合に使用される濃度測定装置1は、図1で示されているものである。
2 測定セル
3 受発光部
4 試料供給ライン
5,5A,5B 試薬供給ライン
6 液排出ライン
7 演算処理装置
8 出力装置
9 制御装置
31 発光体
32 受光体
S0 調整液
S1 試料液(試料)
S2 被測定液
A0 他の領域成分光の基準の吸光度の値
A3 他の領域成分光の、着色剤のみに起因する吸光度の値
T0,T1,T2 試薬
T0c,T1c,T2c 着色剤入り試薬
Claims (6)
- 試料への試薬の添加により発色した被測定液に可視光域を含む光を透過させて、その透過光のうち、前記可視光域の光を略3分割して得られるレッド領域成分の光、グリーン領域成分の光、又はブルー領域成分の光の何れかから選定されるか、又は、これらを組み合わせた複数の前記領域成分の光から選定される溶解物濃度測定用の前記領域成分の光を、吸収することなく透過させる色に前記被測定液を発色させる着色剤を、前記試薬に加えて着色剤入り試薬を作る試薬調製工程と、
前記着色剤入り試薬が添加されている前記被測定液からの前記透過光に基づいて、前記溶解物濃度測定用の前記領域成分の光の吸光度を算出することにより、前記試料中の特定の溶解物の濃度を測定する濃度測定工程と、
前記着色剤入り試薬が添加されている前記被測定液からの前記透過光に基づいて、前記溶解物濃度測定用の前記領域成分の光以外の、他の前記領域成分の光の吸光度を算出することにより、この他の前記領域成分の光の、前記着色剤のみに起因する吸光度の値を定め、この吸光度の値と基準の吸光度の値とを比較することにより、前記試薬が必要量だけ添加されているか否かの判定を行う判定工程とを有することを特徴とする溶解物濃度の測定方法。 - 前記試料に2種類の前記試薬が添加される場合には、前記試薬調製工程において、3つの前記領域成分の光のうち、前記溶解物濃度測定用の前記領域成分の光と残りの前記領域成分の光の一方とを吸収することなく透過させる色に、前記被測定液を発色させる第1の着色剤を、前記試薬の一方に加えて第1の着色剤入り試薬を作るとともに、前記溶解物濃度測定用の前記領域成分の光と前記残りの前記領域成分の光の他方とを吸収することなく透過させる色に、前記被測定液を発色させる第2の着色剤を、前記試薬の他方に加えて第2の着色剤入り試薬を作ることを特徴とする請求項1記載の溶解物濃度の測定方法。
- 前記被測定液が、前記特定の溶解物の濃度の変化に伴って変色するように発色し、2つの前記領域成分の光が前記溶解物濃度測定用の前記領域成分の光となる場合には、前記試薬調製工程において、前記2つの前記領域成分の光を吸収することなく透過させる色に、前記被測定液を発色させる着色剤を、前記試薬に加えて着色剤入り試薬を作ることを特徴とする請求項1記載の溶解物濃度の測定方法。
- 前記試薬が必要量だけ添加されていないと判定された場合には、前記着色剤入り試薬の再添加を行いつつ、前記濃度測定工程と前記判定工程とを繰り返すことを特徴とす請求項1乃至3の何れかに記載の溶解物濃度の測定方法。
- 前記試薬が必要量だけ添加されていないと判定された後、所定の時間経過しても、前記試薬が必要量だけ添加されていないと判定された場合には、前記判定工程において、警報を発して測定を中止することを特徴とする請求項4記載の溶解物濃度の測定方法。
- 前記試薬が必要量だけ添加されていないと判定された場合には、前記判定工程において、警報を発して測定を中止することを特徴とする請求項1乃至3の何れかに記載の溶解物濃度の測定方法。
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