WO2014128956A1 - 電気泳動用キャピラリユニット及びそのキャピラリユニットを備えた電気泳動装置 - Google Patents
電気泳動用キャピラリユニット及びそのキャピラリユニットを備えた電気泳動装置 Download PDFInfo
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- WO2014128956A1 WO2014128956A1 PCT/JP2013/054717 JP2013054717W WO2014128956A1 WO 2014128956 A1 WO2014128956 A1 WO 2014128956A1 JP 2013054717 W JP2013054717 W JP 2013054717W WO 2014128956 A1 WO2014128956 A1 WO 2014128956A1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44743—Introducing samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44791—Microapparatus
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
Definitions
- the present invention relates to a capillary unit including a capillary used as an electrophoresis channel for electrophoresis analysis and an electrophoresis apparatus including the capillary unit.
- an electrophoresis apparatus has been used to analyze a very small amount of protein or nucleic acid.
- Typical examples of the electrophoresis apparatus include a microchip electrophoresis apparatus and a capillary electrophoresis apparatus.
- the microchip electrophoresis apparatus is an apparatus that uses a microchip having a fine channel inside a substrate and having wells and reservoirs formed at both ends of the channel.
- the microchip is horizontally arranged and adjusted to a constant temperature, and an autosampler, a polymer (separation medium) filling mechanism, a suction nozzle, an electrode and the like access the wells and reservoirs at both ends of the flow path to the capillary.
- the separation medium is filled, the sample is introduced, the buffer solution is filled into the reservoir, and the electrophoretic analysis is performed (see, for example, Patent Document 1).
- a capillary electrophoresis apparatus is an apparatus that uses a capillary as an electrophoresis channel.
- a capillary is horizontally arranged, and members having reservoirs are fixed at both ends of the capillary, and the capillary is connected to the capillary via the member. Achieve separation medium filling, sample introduction, and electrophoretic analysis.
- the capillary is bent halfway, the anode end side is horizontally arranged, an anode reservoir equipped with a separation medium filling mechanism or the like is fixed to the anode end, and the cathode end side is fixed.
- an electrophoresis apparatus which is arranged vertically downward and opened so that a cathode end of a capillary can directly access a sample storage portion in which a sample is stored. In such an electrophoresis apparatus, since the cathode end of the capillary can directly access the sample, an autosampler for introducing the sample into the capillary is unnecessary.
- the capillary in order to curve the capillary, the capillary needs to have a certain length, and there is a limit to shortening the length of the capillary when speeding up the electrophoresis.
- the detection peak band becomes broad and the separation performance deteriorates.
- the influence of the difference in the migration length of the curved portion of the capillary can be reduced by increasing the capillary length, it is not possible to increase the speed of electrophoresis.
- an electrophoresis device that installs a reservoir, capillary, and electrode cartridge that are pre-sealed with a separation medium so that the capillary is vertical and allows the lower end of the cartridge capillary to directly access the sample and buffer solution.
- the separation medium is preliminarily filled in the capillary of the cartridge, and the sample is introduced by directly accessing the sample at the lower end of the capillary. It is unnecessary. Since the capillary of the cartridge is straight and has a short capillary length, electrophoresis can be performed at high speed. However, since the separation medium is prefilled in the capillary of the cartridge, the manufacturing cost of the cartridge is high, and the separation medium in the capillary cannot be replaced, so that the number of times of use is limited. Therefore, the cartridge is an expensive disposable item.
- an object of the present invention is to make it possible to accurately and easily fill a separation medium into a migration channel and to form a sample plug, and to shorten the length of a capillary to increase the speed of electrophoresis. It is what.
- a capillary unit includes a reservoir composed of a recess capable of storing a liquid, a linear capillary having one end fixed to the bottom side of the reservoir and extending in a direction opposite to the opening of the reservoir, A nozzle connecting portion that is provided between the bottom and one end of the capillary, and that removably connects a nozzle for injecting liquid into the capillary from the reservoir side while maintaining liquid tightness.
- the electrophoresis apparatus has a capillary unit installation portion in which the capillary unit of the present invention is installed with the capillary vertical, and a nozzle that discharges a separation medium from the tip, and the nozzle is installed in the capillary unit installation portion
- a separation medium filling mechanism that connects to the nozzle connection portion of the capillary unit and fills the capillary with a separation medium
- a buffer liquid supply mechanism that supplies the buffer liquid to the reservoir of the capillary unit, a sample is contained therein, and the lower end of the capillary is
- the upper part opens so as to contact the sample, and when the lower end of the capillary is brought into contact with the sample, the sample storage part is positioned below the capillary unit installation part, the buffer liquid is stored therein, and the lower end of the capillary is used as the buffer liquid.
- a buffer reservoir that is positioned below the capillary unit installation portion when being brought into contact with the electrode, an electrode for applying a voltage to both ends of the capillary, and a detector that optically detects a sample migrating in the capillary Is.
- one end of a linear capillary is fixed to the bottom side of a reservoir comprising a recess capable of storing a liquid, the capillary extends in a direction opposite to the opening of the reservoir, and the bottom of the reservoir and the capillary
- the nozzle unit for connecting the nozzle for injecting the liquid from the reservoir side into the capillary is detachably connected between the one end and the end of the nozzle so that the configuration of the capillary unit is simple. Therefore, it can be manufactured at a low cost and can be easily attached to and detached from the electrophoresis apparatus. Since the capillaries are linear, it is possible to shorten the capillary length and increase the speed of electrophoresis.
- the capillary unit of the present invention is provided with a capillary unit installation portion for installing the capillary unit vertically, and the capillary is filled with the separation medium from the upper end side of the capillary with the lower end of the capillary open.
- the sample is introduced into the capillary by directly accessing the sample and buffer solution, so that the excess separation medium and sample are removed by suction. No work is required.
- FIG. 5 is a cross-sectional view taken along the line XX in FIG. It is a block diagram which shows roughly the control system of the Example. It is a flowchart which shows an example of operation
- a plurality of capillaries and a plurality of reservoirs individually corresponding to the capillaries may be provided, and these reservoirs may be integrated. Then, a multicapillary electrophoresis apparatus can be realized using the capillary unit of the present invention.
- a plurality of capillaries may be provided, and each capillary may be connected to a common reservoir via an individual nozzle connection portion.
- a multi-capillary electrophoresis apparatus can be realized using the capillary unit of the present invention.
- the capillary unit installation portion includes a heat conductive block for holding a portion other than the lower end portion of the capillary and a heater for heating the block. If it does so, the temperature control of the capillary which is an electrophoresis channel will be attained, and the separation performance can be stabilized.
- the heater is preferably a rubber heater attached to the entire surface of the block. Since the capillary unit installation part is used to install the capillary vertically, the length in the vertical direction becomes long and a temperature gradient is likely to be formed. For this reason, by attaching a rubber heater to the entire surface of the block constituting the capillary unit installation portion so as to heat the entire surface of the block, it becomes difficult to form a temperature gradient in the vertical direction on the block, and the temperature of the capillary The uniformity of control can be improved.
- the sample storage portion and the buffer reservoir are provided on a moving stage that can move in the horizontal plane direction and the vertical direction, and the tip of the capillary is placed on the sample in the sample storage portion.
- the movement of bringing the capillary into contact with the buffer solution in the buffer reservoir is performed by moving the stage.
- control unit for controlling the operation of the electrophoresis apparatus, after introducing the sample into the capillary, filling the capillary with the separation medium, and filling the reservoir of the capillary unit with the buffer solution, You may provide the control part comprised so that the lower end of a capillary may contact the sample of a sample storage part, and it may apply by applying a voltage to the both ends of a capillary.
- the sample is introduced into the capillary, the nozzle is connected to the nozzle connection part of the capillary unit and the capillary is filled with the separation medium, and then the nozzle is connected to the nozzle connection part.
- a control unit configured to insert the lower end of the capillary into the sample storage unit in a connected state and suck the separation medium in the capillary with a suction nozzle may be further provided.
- FIG. 1 is a partial sectional view showing an embodiment of a capillary unit.
- the capillary 2 is shown in a front view, and a portion other than the capillary 2 is shown in a sectional view.
- the capillary unit 1 includes a linear capillary 2 and a reservoir block 4.
- the reservoir block 4 includes a reservoir 8 formed of a recess that can store a liquid.
- One end of the capillary 2 is fixed by a ferrule 6 on the bottom side of the reservoir 8 of the reservoir block 4 so as to extend in a direction opposite to the opening of the reservoir 8.
- the material of the reservoir block 4 is, for example, polybutylene terephthalate (PBT).
- PBT polybutylene terephthalate
- the capillary 2 may be fixed to the reservoir block 4 by bonding with an adhesive.
- a part of the capillary 2 serves as a detection position 2a, and the protective film covering the surface of the capillary 2 is removed, and optical measurement in the capillary 2 such as absorbance measurement and fluorescence measurement is possible.
- the reservoir block 4 is connected to the bottom of the reservoir 8 and one end of the capillary 2, and a nozzle for injecting a liquid from the reservoir 8 into the capillary 2 is connected in a liquid-tight manner.
- Part 10 is provided.
- FIG. 2 is a partial sectional view showing another embodiment of the capillary unit. Also in this figure, the capillary 2 is shown by a front view, and parts other than the capillary 2 are shown by sectional views.
- the capillary unit 1a of this embodiment is composed of a plurality of capillaries 2 and a reservoir block 4a.
- the reservoir block 4 a is provided with a reservoir 8 corresponding to each capillary 2, and one end of each capillary 2 is fixed to the reservoir block 4 a with ferrule 6 on the bottom side of each reservoir 8.
- a nozzle connection 10 is provided between the bottom of each reservoir 8 and one end of the capillary 2, and the nozzle for injecting the liquid can be detachably connected while maintaining liquid tightness.
- the capillary 2 may be fixed to the reservoir block 4a by bonding with an adhesive.
- FIG. 3 is a partial sectional view showing still another embodiment of the capillary unit.
- the capillary 2 is shown by a front view, and parts other than the capillary 2 are shown by sectional views.
- the capillary unit 1b of this embodiment is composed of a plurality of capillaries 2 and a reservoir block 4b.
- the reservoir block 4 b is provided with a reservoir 9 common to all the capillaries 2, and one end of each capillary 2 is fixed to the reservoir block 4 b with ferrule 6 on the bottom side of the common reservoir 9.
- a nozzle connecting portion 10 is provided between the bottom of the reservoir 9 and one end of each capillary 2, and the nozzle for injecting the liquid can be detachably connected while maintaining liquid tightness.
- the capillary 2 may be fixed to the reservoir block 4a by bonding with an adhesive.
- the capillary unit 1 described with reference to FIG. 1 is used.
- the capillary unit 1a described with reference to FIG. 2 and the capillary unit 1b described with reference to FIG. It can be applied to the device.
- a capillary unit installation section 12 for installing the capillary unit 1 is provided.
- the capillary unit installation unit 12 holds the capillary unit 1 so that the capillary 2 is vertical.
- the lower end portion of the capillary 2 protrudes downward from the capillary unit installation portion 12, and the lower end portion of the capillary 2 can directly access the inside of a sample tube 28 and a buffer reservoir 30, which will be described later.
- the capillary unit installation unit 12 is provided with a heater 13 and a temperature sensor 14, and the temperature of the capillary 2 is controlled to a constant temperature.
- the capillary unit installation portion 12 is composed of two blocks 12-1 and 12-2 made of a metal having good thermal conductivity such as aluminum, for example. A part of the ferrule 6 of the capillary unit 12 and a part of the capillary 2 (excluding the lower end part) are sandwiched and held between 12-2. Grooves into which the capillary unit 1 is fitted are formed on the inner surfaces of the two blocks.
- the heater 13 is a sheet-like rubber heater, and is attached to the entire surface of one block 12-1 constituting the capillary unit installation unit 12.
- Holes 12 a and 13 a are provided at predetermined positions of the block 12-1 and the heater 13, respectively, and the sample component that migrates in the capillary 2 at the detection position 2 a of the capillary 2 can be optically detected by the detection unit 15. It can be done.
- the detection unit 15 includes a detector and a light source that are arranged opposite to each other with the optical measurement unit 2a of the capillary 2 interposed therebetween, and detects a change in absorbance in the capillary 2 from the intensity of light transmitted through the capillary 2.
- the optical measurement unit 2a is irradiated with excitation light from a light source, and fluorescence from a component excited by the excitation light is detected by a detector.
- the detection signal obtained by the detection unit 15 is taken into the calculation unit 20 to identify the sample components.
- the computing unit 20 is realized by, for example, a personal computer (PC) connected to the electrophoresis apparatus or a dedicated computer provided in the electrophoresis apparatus.
- a detection unit 15 that performs optical detection for each capillary 2 may be provided individually.
- One detection unit 15 and a mechanism for moving the detection unit 15 in the horizontal direction are provided, and the detection unit 15 is sequentially moved to a measurement position where optical detection is performed on each capillary 2 to sequentially perform optical detection. Good.
- a separation medium filling mechanism 22 and a buffer liquid supply mechanism 24 are provided.
- the separation medium filling mechanism 22 is a mechanism for filling the capillary 2 with a polymer as a separation medium.
- the buffer liquid supply mechanism 24 is a mechanism for supplying the buffer liquid to the reservoir 8 of the capillary unit 1.
- the separation medium filling mechanism 22 has a nozzle 22a and a syringe pump 22b, and the nozzle 22a and the syringe pump 22b are connected via a tube.
- the nozzle 22 a can move in the horizontal direction and the vertical direction, and can be connected to the capillary 2 by inserting the tip into the nozzle connection portion 10 provided in the reservoir block 4 of the capillary unit 1.
- the buffer liquid supply mechanism 24 has a nozzle 24a and a syringe pump 24b, and the nozzle 24a and the syringe pump 24b are connected via a tube.
- the nozzle 24a can move in the horizontal direction and the vertical direction.
- a moving stage 26 is provided below the capillary unit installation section 12.
- a sample tube 28 sample storage unit
- a buffer reservoir 30 are placed on the moving stage 26, and a drain port 32 is mounted.
- the moving stage 26 can be moved in the horizontal direction and the vertical direction by the stage driving mechanism 27, and any one of the sample tube 28, the buffer reservoir 30, or the drain port 32 can be accessed with respect to the lower end portion of the capillary 2. it can.
- the sample tube 28 contains a sample inside
- the buffer reservoir 30 contains a buffer solution inside.
- a drain tube 34 is connected to the drain port 32, and unnecessary liquid can be discharged through the drain port 32.
- Both the sample tube 28 and the buffer reservoir 30 have open upper surfaces, and the lower end of the capillary 2 can be accessed by the movement of the moving stage 26 to the sample in the sample tube 28 or the buffer solution in the buffer reservoir 30.
- the electrophoresis apparatus is arranged so that the end is inserted into the sample tube 28 and the buffer reservoir 30 together with the electrode 16 having the end inserted into the reservoir 8 of the capillary unit 1 and the lower end of the capillary 2.
- An electrode 18 is provided.
- the electrophoresis apparatus includes a nozzle 22 a of the separation medium filling mechanism 22, a separation medium filling mechanism driving unit 36 that drives the syringe pump 22 b, and a nozzle 24 a of the buffer liquid supply mechanism 24. And a buffer liquid supply mechanism driving unit 38 for driving the syringe pump 24b, a stage driving mechanism 42 for driving the moving stage 26, and a voltage applying unit 44 for applying a voltage to the electrodes 16 and 18. These are controlled by the controller 46 together with the heater 13.
- the analyst inputs information such as sample information and analysis conditions to the calculation unit 20.
- the computing unit 20 gives information such as analysis conditions to the control unit 46 based on the information input by the analyst.
- the control unit 46 controls the operation by giving control signals to the separation medium filling mechanism driving unit 36, the buffer liquid supply mechanism driving unit 38, the stage driving mechanism 42, and the voltage applying unit 44 based on the information given by the calculation unit 20. . Further, the control unit 46 takes in a detection signal from the temperature sensor 14 provided in the capillary unit installation unit 12 and controls the output of the heater 13 so that the temperature of the capillary 2 becomes a constant temperature.
- the calculation unit 20 receives the detection signal obtained by the detection unit 15, and the calculation unit 20 executes detection of a change in absorbance in the capillary 2 or identification of a sample component by fluorescence detection from the capillary 2. .
- the lower end of the capillary 2 is placed in the drain port 32 by the movement of the moving stage 26.
- the nozzle 22a is connected to the nozzle connection portion 10 of the capillary unit 1 while the polymer is sucked into the syringe pump 22b.
- the capillary 2 is filled with the polymer (step S1).
- the polymer filled in the capillary 2 may be prepared in advance by the syringe pump 22b sucking a large amount of polymer in advance, or the syringe pump 22b in a state in which a certain amount of water is sucked is used in the capillary 2. It may have been sucked from a container containing a polymer during the filling operation of the separation medium.
- the polymer is sucked by the syringe pump 22b in a state where a certain amount of water is sucked, air is sucked before the polymer is sucked to form an air gap between the water and the polymer. Can be prevented from being mixed.
- the nozzle 22a is moved to a position different from the reservoir 8 while the buffer liquid is sucked into the syringe pump 24b, and the nozzle 24a of the buffer liquid supply mechanism 24 is moved to the reservoir. 8 Position to the top.
- the syringe pump 24b is driven to discharge to discharge the buffer liquid from the tip of the nozzle 24a, thereby supplying the buffer liquid to the reservoir 8 (step S2).
- the end of the electrode 16 is disposed in the reservoir 8 in advance, and the end of the electrode 16 is inserted into the buffer solution when the reservoir 8 is filled with the buffer solution.
- the supply of the buffer solution to the reservoir 8 may be performed by discharging the buffer solution that has been sucked in advance into the syringe pump 24 from the nozzle 24a, or the buffer solution in the buffer reservoir 30 may be discharged from the nozzle 24a. You may make it inhale each time through.
- the sample tube 28 in which the sample to be analyzed is accommodated by the movement of the moving stage 26 is placed at the lower end position of the capillary 2, and the lower end of the capillary 2 is accessed to the sample to be analyzed.
- the electrode 18 is also inserted into the sample to be analyzed together with the lower end of the capillary 2.
- a sample is electrically introduced into the capillary 2 by applying a predetermined voltage between the electrodes 16 and 18 (step S3).
- the buffer reservoir 30 is arranged at the lower end of the capillary 2 by the movement of the moving stage 26, and the lower end of the capillary 2 is accessed to the buffer solution (step S4).
- the electrode 18 is also inserted into the buffer solution together with the lower end of the capillary 2. Electrophoresis of the sample is executed by applying a predetermined voltage between the electrodes 16 and 18 (step S5). Each component contained in the sample has a different migration speed depending on its molecular weight, and passes through the detection position 2a in order from the component having the smallest molecular weight.
- FIG. 8 is a flowchart showing an example of the operation when the sample is introduced into the capillary 2 using the separation medium filling mechanism 22. In this operation, the introduction of the separation medium into the capillary 2 is the same as the operation described with reference to FIG.
- step S11 After the introduction of the separation medium into the capillary 2 is completed (step S11), the moving stage 26 is moved while the nozzle 22a is connected to the nozzle connecting portion 10, and the lower end of the capillary 2 is accessed to the sample to be analyzed.
- the syringe pump 22b is inhaled by a predetermined amount. Thereby, a predetermined amount of sample is introduced to the lower end side of the capillary 2 (step S12).
- the nozzle 22a is moved to a position different from the reservoir 8 while the buffer liquid is being sucked into the syringe pump 24b, and the nozzle 24a of the buffer liquid supply mechanism 24 is disposed at a position on the reservoir 8 so as to be nozzle 24a.
- the buffer liquid is discharged from the tip of the liquid and supplied to the reservoir 8 (step S13).
- the buffer reservoir 30 is arranged at the lower end position of the capillary 2, and the lower end of the capillary 2 is accessed to the buffer solution (step S14).
- electrophoresis of the sample is executed by applying a predetermined voltage between the electrodes 16 and 18 (step S15).
- FIG. 9 shows another embodiment of the electrophoresis apparatus.
- the separation medium filling mechanism 22 and the buffer liquid supply mechanism 24 of FIG. 4 are realized by a common liquid suction / discharge mechanism 50.
- the liquid suction / discharge mechanism 50 includes a nozzle 50a and a syringe pump 50b that can move in the horizontal and vertical directions.
- a separation medium container 54 containing a separation medium is disposed at a position where the nozzle 36a can move.
- the syringe pump 50 b is connected to the nozzle 50 a and the cleaning liquid container 52 via the switching valve 56. Both the separation medium and the buffer solution are sucked by the nozzle 50 a and the syringe pump 50 b and injected into the capillary 2 or the reservoir 8. Further, the inside of the flow path of the liquid suction / discharge mechanism 50 can be cleaned as necessary.
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Abstract
Description
この実施例のキャピラリユニット1aは、複数のキャピラリ2とリザーバブロック4aにより構成されている。リザーバブロック4aには各キャピラリ2に対応するリザーバ8が設けられており、各キャピラリ2の一端がそれぞれのリザーバ8の底部側においてリザーバブロック4aにフェルル6によって固定されている。
この実施例のキャピラリユニット1bは、複数のキャピラリ2とリザーバブロック4bにより構成されている。リザーバブロック4bには全てのキャピラリ2に共通のリザーバ9が設けられており、各キャピラリ2の一端が共通のリザーバ9の底部側においてリザーバブロック4bにフェルル6によって固定されている。
この電気泳動装置には、図4において図示は省略されているが、分離媒体充填機構22のノズル22aとシリンジポンプ22bを駆動する分離媒体充填機構駆動部36と、バッファ液供給機構24のノズル24aとシリンジポンプ24bを駆動するバッファ液供給機構駆動部38と、移動ステージ26を駆動するステージ駆動機構42と、電極16,18に電圧を印加する電圧印加部44が設けられている。これらはヒータ13とともに制御部46により制御されている。
移動ステージ26の移動によりキャピラリ2の下端をドレインポート32内に配置する。シリンジポンプ22b内にポリマーが吸入されている状態で、ノズル22aをキャピラリユニット1のノズル接続部10に接続する。シリンジポンプ22bを吐出駆動してノズル22aの先端からポリマーを吐出することにより、キャピラリ2にポリマーを充填する(ステップS1)。
2 キャピラリ
2a 検出位置
4,4a,4b リザーバブロック
6 フェルル
8,9 リザーバ
10 ノズル接続部
12,12-1,12-2 キャピラリユニット設置部
12a,13a 検出用の穴
13 ヒータ
14 温度センサ
15 検出部
16,18 電極
20 演算部
22 分離媒体充填機構
22a,24a、50a ノズル
22b,24b、50b シリンジポンプ
24 バッファ液供給機構
26 移動ステージ
27 ステージ駆動機構
28 サンプルチューブ
30 バッファリザーバ
32 ドレインポート
34 ドレインチューブ
36 分離媒体充填機構駆動部
38 バッファ液供給機構駆動部
42 ステージ駆動機構
44 電圧印加部
46 制御部
50 液吸入吐出機構
52 洗浄液容器
54 分離媒体容器
56 切換バルブ
Claims (9)
- 液を貯留することができる凹部からなるリザーバと、
一端が前記リザーバの底部側に固定され、前記リザーバの開口部と反対の方向へ延びた直線形状のキャピラリと、
前記リザーバの底部と前記キャピラリの前記一端側端部との間に設けられ、前記リザーバ側から前記キャピラリ内に液を注入するためのノズルを液密を保って着脱可能に接続するノズル接続部と、を備えたキャピラリユニット。 - 複数の前記キャピラリと各キャピラリに個別に対応する複数の前記リザーバが設けられ、前記リザーバが一体化されている請求項1に記載のキャピラリユニット。
- 複数の前記キャピラリが設けられており、各キャピラリが個別の前記ノズル接続部を介して共通のリザーバに接続されている請求項1に記載のキャピラリユニット。
- 請求項1に記載のキャピラリユニットを前記キャピラリを鉛直にして設置するキャピラリユニット設置部と、
先端から分離媒体を吐出するノズルを有し、前記ノズルを前記キャピラリユニット設置部に設置された前記キャピラリユニットのノズル接続部に接続して前記キャピラリに分離媒体を充填する分離媒体充填機構と、
前記キャピラリユニットのリザーバにバッファ液を供給するバッファ液供給機構と、
内部にサンプルを収容し、前記キャピラリの下端をサンプルに接触させるように上方が開口し、前記キャピラリの下端をサンプルに接触させる時に前記キャピラリユニット設置部の下方に位置決めされるサンプル収容部と、
内部にバッファ液を収容し、前記キャピラリの下端をバッファ液に接触させるように上方が開口し、前記キャピラリの下端をバッファ液に接触させる時に前記キャピラリユニット設置部の下方に位置決めされるバッファリザーバと、
前記キャピラリの両端に電圧を印加するための電極と、
前記キャピラリ内を泳動するサンプルを光学的に検出する検出器と、を備えた電気泳動装置。 - 前記キャピラリユニット設置部は、前記キャピラリの下端部以外の部分を保持する熱伝導性のブロック及び前記ブロックを加熱するヒータを備えている請求項4に記載の電気泳動装置。
- 前記ヒータは前記ブロックの一表面全体に貼付されたラバーヒータである請求項5に記載の電気泳動装置。
- 前記サンプル収容部及び前記バッファリザーバは、水平面内方向と鉛直方向へ移動する移動ステージ上に設けられており、
前記キャピラリの先端を前記サンプル収容部内のサンプルに接触させる動作及び前記キャピラリの先端を前記バッファリザーバ内のバッファ液に接触させる動作を前記ステージの移動によって行なう請求項4から6のいずれか一項に記載の電気泳動装置。 - 当該電気泳動装置の動作を制御する制御部であって、前記キャピラリへのサンプルの導入を、前記キャピラリに分離媒体を充填し、前記キャピラリユニットのリザーバにバッファ液を充填した後で、前記キャピラリの下端を前記サンプル収容部のサンプルに接触させ、前記キャピラリの両端に電圧を印加することにより行なうように構成された制御部をさらに備えた請求項4から7のいずれか一項に記載の電気泳動装置。
- 当該電気泳動装置の動作を制御する制御部であって、前記キャピラリへのサンプルの導入を、前記キャピラリユニットの前記ノズル接続部に前記ノズルを接続して前記キャピラリに分離媒体を充填した後、前記ノズル接続部に前記ノズルを接続した状態で前記キャピラリの下端を前記サンプル収容部内に挿入し、前記吸入ノズルで前記キャピラリ内の分離媒体を吸入することにより行なうように構成された制御部をさらに備えた請求項4から7のいずれか一項に記載の電気泳動装置。
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