WO2014091386A2 - An improved process for preparation of minodronic acid - Google Patents
An improved process for preparation of minodronic acid Download PDFInfo
- Publication number
- WO2014091386A2 WO2014091386A2 PCT/IB2013/060737 IB2013060737W WO2014091386A2 WO 2014091386 A2 WO2014091386 A2 WO 2014091386A2 IB 2013060737 W IB2013060737 W IB 2013060737W WO 2014091386 A2 WO2014091386 A2 WO 2014091386A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- formula
- compound
- acid
- reaction mixture
- minodronic acid
- Prior art date
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- 229950011129 minodronic acid Drugs 0.000 title claims abstract description 59
- VMMKGHQPQIEGSQ-UHFFFAOYSA-N minodronic acid Chemical compound C1=CC=CN2C(CC(O)(P(O)(O)=O)P(O)(O)=O)=CN=C21 VMMKGHQPQIEGSQ-UHFFFAOYSA-N 0.000 title claims abstract description 55
- 238000000034 method Methods 0.000 title claims abstract description 54
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- UTCSSFWDNNEEBH-UHFFFAOYSA-N imidazo[1,2-a]pyridine Chemical compound C1=CC=CC2=NC=CN21 UTCSSFWDNNEEBH-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000011541 reaction mixture Substances 0.000 claims description 48
- 238000006243 chemical reaction Methods 0.000 claims description 38
- 150000001875 compounds Chemical class 0.000 claims description 18
- 239000002904 solvent Substances 0.000 claims description 17
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 15
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- FAIAAWCVCHQXDN-UHFFFAOYSA-N phosphorus trichloride Chemical compound ClP(Cl)Cl FAIAAWCVCHQXDN-UHFFFAOYSA-N 0.000 claims description 14
- 239000002253 acid Substances 0.000 claims description 13
- AVQQQNCBBIEMEU-UHFFFAOYSA-N 1,1,3,3-tetramethylurea Chemical compound CN(C)C(=O)N(C)C AVQQQNCBBIEMEU-UHFFFAOYSA-N 0.000 claims description 12
- 239000002585 base Substances 0.000 claims description 12
- -1 Minodronic acid compound Chemical class 0.000 claims description 10
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 10
- 239000013078 crystal Substances 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 10
- 238000001816 cooling Methods 0.000 claims description 9
- ISIJQEHRDSCQIU-UHFFFAOYSA-N tert-butyl 2,7-diazaspiro[4.5]decane-7-carboxylate Chemical compound C1N(C(=O)OC(C)(C)C)CCCC11CNCC1 ISIJQEHRDSCQIU-UHFFFAOYSA-N 0.000 claims description 9
- 150000003839 salts Chemical class 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 6
- 125000003545 alkoxy group Chemical group 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- NWZSZGALRFJKBT-KNIFDHDWSA-N (2s)-2,6-diaminohexanoic acid;(2s)-2-hydroxybutanedioic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O.NCCCC[C@H](N)C(O)=O NWZSZGALRFJKBT-KNIFDHDWSA-N 0.000 claims description 4
- GUGQQGROXHPINL-UHFFFAOYSA-N 2-oxobutanoyl chloride Chemical compound CCC(=O)C(Cl)=O GUGQQGROXHPINL-UHFFFAOYSA-N 0.000 claims description 4
- SECXISVLQFMRJM-UHFFFAOYSA-N N-Methylpyrrolidone Chemical compound CN1CCCC1=O SECXISVLQFMRJM-UHFFFAOYSA-N 0.000 claims description 4
- IKDUDTNKRLTJSI-UHFFFAOYSA-N hydrazine monohydrate Substances O.NN IKDUDTNKRLTJSI-UHFFFAOYSA-N 0.000 claims description 4
- 239000002798 polar solvent Substances 0.000 claims description 4
- 238000010992 reflux Methods 0.000 claims description 4
- 229910052736 halogen Inorganic materials 0.000 claims description 3
- 150000002367 halogens Chemical class 0.000 claims description 3
- 125000000951 phenoxy group Chemical group [H]C1=C([H])C([H])=C(O*)C([H])=C1[H] 0.000 claims description 3
- CYSGHNMQYZDMIA-UHFFFAOYSA-N 1,3-Dimethyl-2-imidazolidinon Chemical compound CN1CCN(C)C1=O CYSGHNMQYZDMIA-UHFFFAOYSA-N 0.000 claims description 2
- GUVUOGQBMYCBQP-UHFFFAOYSA-N dmpu Chemical compound CN1CCCN(C)C1=O GUVUOGQBMYCBQP-UHFFFAOYSA-N 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- LMBFAGIMSUYTBN-MPZNNTNKSA-N teixobactin Chemical compound C([C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CO)C(=O)N[C@H](CCC(N)=O)C(=O)N[C@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CO)C(=O)N[C@H]1C(N[C@@H](C)C(=O)N[C@@H](C[C@@H]2NC(=N)NC2)C(=O)N[C@H](C(=O)O[C@H]1C)[C@@H](C)CC)=O)NC)C1=CC=CC=C1 LMBFAGIMSUYTBN-MPZNNTNKSA-N 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims 4
- 150000004649 carbonic acid derivatives Chemical class 0.000 claims 2
- 238000005406 washing Methods 0.000 claims 2
- 150000008044 alkali metal hydroxides Chemical class 0.000 claims 1
- 150000004703 alkoxides Chemical class 0.000 claims 1
- 238000001035 drying Methods 0.000 claims 1
- 150000004679 hydroxides Chemical class 0.000 claims 1
- 238000002156 mixing Methods 0.000 claims 1
- 239000000243 solution Substances 0.000 description 21
- 239000012535 impurity Substances 0.000 description 13
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 12
- 239000003085 diluting agent Substances 0.000 description 10
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 9
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 9
- XHXFXVLFKHQFAL-UHFFFAOYSA-N phosphoryl trichloride Chemical compound ClP(Cl)(Cl)=O XHXFXVLFKHQFAL-UHFFFAOYSA-N 0.000 description 8
- 239000000047 product Substances 0.000 description 7
- AFVFQIVMOAPDHO-UHFFFAOYSA-N Methanesulfonic acid Chemical compound CS(O)(=O)=O AFVFQIVMOAPDHO-UHFFFAOYSA-N 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 5
- 238000000746 purification Methods 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- ICSNLGPSRYBMBD-UHFFFAOYSA-N 2-aminopyridine Chemical compound NC1=CC=CC=N1 ICSNLGPSRYBMBD-UHFFFAOYSA-N 0.000 description 4
- 208000006386 Bone Resorption Diseases 0.000 description 4
- 150000007513 acids Chemical class 0.000 description 4
- 230000024279 bone resorption Effects 0.000 description 4
- MVPPADPHJFYWMZ-UHFFFAOYSA-N chlorobenzene Chemical compound ClC1=CC=CC=C1 MVPPADPHJFYWMZ-UHFFFAOYSA-N 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- SGNOGHAGXLAOHG-UHFFFAOYSA-N 2-imidazo[1,2-a]pyridin-3-ylacetic acid;hydrochloride Chemical compound Cl.C1=CC=CN2C(CC(=O)O)=CN=C21 SGNOGHAGXLAOHG-UHFFFAOYSA-N 0.000 description 3
- BXWLVQXAFBWKSR-UHFFFAOYSA-N 2-methoxy-5-methylsulfonylbenzoic acid Chemical compound COC1=CC=C(S(C)(=O)=O)C=C1C(O)=O BXWLVQXAFBWKSR-UHFFFAOYSA-N 0.000 description 3
- 229940122361 Bisphosphonate Drugs 0.000 description 3
- WMFOQBRAJBCJND-UHFFFAOYSA-M Lithium hydroxide Chemical compound [Li+].[OH-] WMFOQBRAJBCJND-UHFFFAOYSA-M 0.000 description 3
- 208000001132 Osteoporosis Diseases 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 150000004663 bisphosphonates Chemical class 0.000 description 3
- 239000000543 intermediate Substances 0.000 description 3
- 229940098779 methanesulfonic acid Drugs 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 239000003643 water by type Substances 0.000 description 3
- ZVBVKRNOISRONE-UHFFFAOYSA-N 2-imidazo[1,2-a]pyridin-3-ylacetic acid Chemical compound C1=CC=CN2C(CC(=O)O)=CN=C21 ZVBVKRNOISRONE-UHFFFAOYSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical class [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 2
- PHSPJQZRQAJPPF-UHFFFAOYSA-N N-alpha-Methylhistamine Chemical compound CNCCC1=CN=CN1 PHSPJQZRQAJPPF-UHFFFAOYSA-N 0.000 description 2
- QWZRZYWLWTWVLF-UHFFFAOYSA-N O.OP(O)=O Chemical compound O.OP(O)=O QWZRZYWLWTWVLF-UHFFFAOYSA-N 0.000 description 2
- 208000010191 Osteitis Deformans Diseases 0.000 description 2
- 208000027868 Paget disease Diseases 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- WQDUMFSSJAZKTM-UHFFFAOYSA-N Sodium methoxide Chemical compound [Na+].[O-]C WQDUMFSSJAZKTM-UHFFFAOYSA-N 0.000 description 2
- 125000000217 alkyl group Chemical group 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 125000004185 ester group Chemical group 0.000 description 2
- 208000027202 mammary Paget disease Diseases 0.000 description 2
- OHZZTXYKLXZFSZ-UHFFFAOYSA-I manganese(3+) 5,10,15-tris(1-methylpyridin-1-ium-4-yl)-20-(1-methylpyridin-4-ylidene)porphyrin-22-ide pentachloride Chemical compound [Cl-].[Cl-].[Cl-].[Cl-].[Cl-].[Mn+3].C1=CN(C)C=CC1=C1C(C=C2)=NC2=C(C=2C=C[N+](C)=CC=2)C([N-]2)=CC=C2C(C=2C=C[N+](C)=CC=2)=C(C=C2)N=C2C(C=2C=C[N+](C)=CC=2)=C2N=C1C=C2 OHZZTXYKLXZFSZ-UHFFFAOYSA-I 0.000 description 2
- 125000003431 oxalo group Chemical group 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- UXCDUFKZSUBXGM-UHFFFAOYSA-N phosphoric tribromide Chemical compound BrP(Br)(Br)=O UXCDUFKZSUBXGM-UHFFFAOYSA-N 0.000 description 2
- OJMIONKXNSYLSR-UHFFFAOYSA-N phosphorous acid Chemical compound OP(O)O OJMIONKXNSYLSR-UHFFFAOYSA-N 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 229920001515 polyalkylene glycol Polymers 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- MFRIHAYPQRLWNB-UHFFFAOYSA-N sodium tert-butoxide Chemical compound [Na+].CC(C)(C)[O-] MFRIHAYPQRLWNB-UHFFFAOYSA-N 0.000 description 2
- 238000000371 solid-state nuclear magnetic resonance spectroscopy Methods 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- OGFAWKRXZLGJSK-UHFFFAOYSA-N 1-(2,4-dihydroxyphenyl)-2-(4-nitrophenyl)ethanone Chemical compound OC1=CC(O)=CC=C1C(=O)CC1=CC=C([N+]([O-])=O)C=C1 OGFAWKRXZLGJSK-UHFFFAOYSA-N 0.000 description 1
- MFGOFGRYDNHJTA-UHFFFAOYSA-N 2-amino-1-(2-fluorophenyl)ethanol Chemical compound NCC(O)C1=CC=CC=C1F MFGOFGRYDNHJTA-UHFFFAOYSA-N 0.000 description 1
- ZFFBIQMNKOJDJE-UHFFFAOYSA-N 2-bromo-1,2-diphenylethanone Chemical compound C=1C=CC=CC=1C(Br)C(=O)C1=CC=CC=C1 ZFFBIQMNKOJDJE-UHFFFAOYSA-N 0.000 description 1
- QSKPIOLLBIHNAC-UHFFFAOYSA-N 2-chloro-acetaldehyde Chemical compound ClCC=O QSKPIOLLBIHNAC-UHFFFAOYSA-N 0.000 description 1
- IEHOLJJUXDEKJG-UHFFFAOYSA-N 2-imidazo[1,2-a]pyridin-3-yl-2-oxoacetic acid Chemical compound C1=CC=CN2C(C(=O)C(=O)O)=CN=C21 IEHOLJJUXDEKJG-UHFFFAOYSA-N 0.000 description 1
- ACQYZSFXPXXIHL-UHFFFAOYSA-N 2-phenylmethoxycarbonyl-3,4-dihydro-1h-isoquinoline-1-carboxylic acid Chemical compound C1CC2=CC=CC=C2C(C(=O)O)N1C(=O)OCC1=CC=CC=C1 ACQYZSFXPXXIHL-UHFFFAOYSA-N 0.000 description 1
- XVCCOEWNFXXUEV-UHFFFAOYSA-N 2-pyridin-3-ylacetic acid;hydrochloride Chemical compound Cl.OC(=O)CC1=CC=CN=C1 XVCCOEWNFXXUEV-UHFFFAOYSA-N 0.000 description 1
- QCQCHGYLTSGIGX-GHXANHINSA-N 4-[[(3ar,5ar,5br,7ar,9s,11ar,11br,13as)-5a,5b,8,8,11a-pentamethyl-3a-[(5-methylpyridine-3-carbonyl)amino]-2-oxo-1-propan-2-yl-4,5,6,7,7a,9,10,11,11b,12,13,13a-dodecahydro-3h-cyclopenta[a]chrysen-9-yl]oxy]-2,2-dimethyl-4-oxobutanoic acid Chemical compound N([C@@]12CC[C@@]3(C)[C@]4(C)CC[C@H]5C(C)(C)[C@@H](OC(=O)CC(C)(C)C(O)=O)CC[C@]5(C)[C@H]4CC[C@@H]3C1=C(C(C2)=O)C(C)C)C(=O)C1=CN=CC(C)=C1 QCQCHGYLTSGIGX-GHXANHINSA-N 0.000 description 1
- OGSPWJRAVKPPFI-UHFFFAOYSA-N Alendronic Acid Chemical compound NCCCC(O)(P(O)(O)=O)P(O)(O)=O OGSPWJRAVKPPFI-UHFFFAOYSA-N 0.000 description 1
- 208000020084 Bone disease Diseases 0.000 description 1
- 0 CN(CCC(*)C1)C1N Chemical compound CN(CCC(*)C1)C1N 0.000 description 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
- 208000037147 Hypercalcaemia Diseases 0.000 description 1
- 208000012659 Joint disease Diseases 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 description 1
- 238000005644 Wolff-Kishner reduction reaction Methods 0.000 description 1
- 238000002441 X-ray diffraction Methods 0.000 description 1
- 229960004343 alendronic acid Drugs 0.000 description 1
- 230000001760 anti-analgesic effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000001754 anti-pyretic effect Effects 0.000 description 1
- 239000012296 anti-solvent Substances 0.000 description 1
- 239000011260 aqueous acid Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 125000003710 aryl alkyl group Chemical group 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- HUCVOHYBFXVBRW-UHFFFAOYSA-M caesium hydroxide Inorganic materials [OH-].[Cs+] HUCVOHYBFXVBRW-UHFFFAOYSA-M 0.000 description 1
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 1
- 239000000920 calcium hydroxide Substances 0.000 description 1
- 229910001861 calcium hydroxide Inorganic materials 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 150000001732 carboxylic acid derivatives Chemical class 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000007810 chemical reaction solvent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000008280 chlorinated hydrocarbons Chemical class 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000001938 differential scanning calorimetry curve Methods 0.000 description 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 1
- 235000011180 diphosphates Nutrition 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000012495 forced degradation study Methods 0.000 description 1
- 150000008282 halocarbons Chemical class 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 1
- 230000000148 hypercalcaemia Effects 0.000 description 1
- 208000030915 hypercalcemia disease Diseases 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000010829 isocratic elution Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 231100001231 less toxic Toxicity 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- AFRJJFRNGGLMDW-UHFFFAOYSA-N lithium amide Chemical compound [Li+].[NH2-] AFRJJFRNGGLMDW-UHFFFAOYSA-N 0.000 description 1
- SIAPCJWMELPYOE-UHFFFAOYSA-N lithium hydride Chemical compound [LiH] SIAPCJWMELPYOE-UHFFFAOYSA-N 0.000 description 1
- 229910000103 lithium hydride Inorganic materials 0.000 description 1
- LZWQNOHZMQIFBX-UHFFFAOYSA-N lithium;2-methylpropan-2-olate Chemical compound [Li+].CC(C)(C)[O-] LZWQNOHZMQIFBX-UHFFFAOYSA-N 0.000 description 1
- VTHJTEIRLNZDEV-UHFFFAOYSA-L magnesium dihydroxide Chemical compound [OH-].[OH-].[Mg+2] VTHJTEIRLNZDEV-UHFFFAOYSA-L 0.000 description 1
- 239000000347 magnesium hydroxide Substances 0.000 description 1
- 229910001862 magnesium hydroxide Inorganic materials 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 230000009401 metastasis Effects 0.000 description 1
- 125000004170 methylsulfonyl group Chemical group [H]C([H])([H])S(*)(=O)=O 0.000 description 1
- 150000004682 monohydrates Chemical class 0.000 description 1
- 239000012454 non-polar solvent Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 210000002997 osteoclast Anatomy 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 238000005954 phosphonylation reaction Methods 0.000 description 1
- UHZYTMXLRWXGPK-UHFFFAOYSA-N phosphorus pentachloride Chemical compound ClP(Cl)(Cl)(Cl)Cl UHZYTMXLRWXGPK-UHFFFAOYSA-N 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- NTTOTNSKUYCDAV-UHFFFAOYSA-N potassium hydride Chemical compound [KH] NTTOTNSKUYCDAV-UHFFFAOYSA-N 0.000 description 1
- 229910000105 potassium hydride Inorganic materials 0.000 description 1
- LPNYRYFBWFDTMA-UHFFFAOYSA-N potassium tert-butoxide Chemical compound [K+].CC(C)(C)[O-] LPNYRYFBWFDTMA-UHFFFAOYSA-N 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 239000012088 reference solution Substances 0.000 description 1
- 206010039073 rheumatoid arthritis Diseases 0.000 description 1
- 238000012776 robust process Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- ODZPKZBBUMBTMG-UHFFFAOYSA-N sodium amide Chemical compound [NH2-].[Na+] ODZPKZBBUMBTMG-UHFFFAOYSA-N 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000012312 sodium hydride Substances 0.000 description 1
- 229910000104 sodium hydride Inorganic materials 0.000 description 1
- CGRKYEALWSRNJS-UHFFFAOYSA-N sodium;2-methylbutan-2-olate Chemical compound [Na+].CCC(C)(C)[O-] CGRKYEALWSRNJS-UHFFFAOYSA-N 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000004885 tandem mass spectrometry Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 125000002088 tosyl group Chemical group [H]C1=C([H])C(=C([H])C([H])=C1C([H])([H])[H])S(*)(=O)=O 0.000 description 1
- 238000005292 vacuum distillation Methods 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
- 238000010626 work up procedure Methods 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
- C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
- C07D471/04—Ortho-condensed systems
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
- C07F9/6561—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing systems of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring or ring system, with or without other non-condensed hetero rings
Definitions
- TITLE AN IMPROVED PROCESS FOR PREPARATION OF MINODRONIC ACID FIELD OF THE INVENTION
- the present invention relates to an improved, commercially viable and industrially advantageous process for the preparation of Minodronic acid.
- the bisphosponic acids and their pharmaceutically acceptable salts are an important class of medicaments that act as specific inhibitor of Osteoclast-mediated bone resorption and are useful in the treatment of bone disorders such as Paget's disease and osteoporosis.
- Bisphosphonates are synthetic analogs of pyrophosphate that bind to the hydroxy-apatite found in the bone.
- Minodronic acid is a compound represented by the following formula (I), which has excellent bone resorption inhibitory activity, anti-inflammatory activity and analgesic- antipyretic activity and is useful for the treatment of diseases in which increased bone resorption participates, such as Paget's disease, hypercalcemia, bone metastasis of cancer and osteoporosis, as well as progress in the bone resorption (induction of osteoporosis) caused by inflammatory joint diseases such as rheumatoid arthritis and the like
- CNl 02020676 also disclose process for preparation of Minodronic
- the synthesis are based on reacting a carboxylic acid with a mixture of phosphorous acid and one of the following phosphorus halides: phosphorus trichloride (PCI3), phosphorus oxychloride (POCI3), phosphorus pentachloride (PCI5), phosphorus tribromide (PBr 3 ), phosphorus oxybromide (POBr 3 ) or phosphorus pentabromide (PBr 5 ), then quenching the reaction mixture with water or a non-oxidizing aqueous acid, followed by heating to hydrolyze the phosphorus intermediates to the final product.
- PCI3 phosphorus trichloride
- POCI3 phosphorus oxychloride
- PCI5 phosphorus pentachloride
- PBr 3 phosphorus tribromide
- POBr 3 phosphorus oxybromide
- PBr 5 phosphorus pentabromide
- U.S. Pat. No. 4,407,761 describes the synthesis of 4-amino-l-hydroxybutylidene-l ,l - bisphosphonic acid (alendronic acid) and other bisphosphonic acids.
- the reaction has been carried out in the presence of diluent, e.g. chlorinated hydrocarbons, especially chlorobenzene, which does not solubilize the reaction components and serves only as a heat carrier.
- the reaction starts as a two-phase system, in which the melted phase gradually thickens into a non-stirrabie mass. This semi solid sticky mass finally turns into a hard, rigid material, thereby coating the walls of the reaction vessel and thus preventing the smooth heat transfer and complicating the product work-up.
- the overall yield of this process is variable i.e. 45% to 56%.
- the solvent i.e. chlorobenzene used in the reaction is carcinogenic in nature and thus not recommendable for industrial scale.
- US. Pat. No. 5,908,959 employs poly alkylene glycols as reaction solvent for synthesizing bisphosphonates.
- the use of poly alkylene glycols on industrial scale is not very feasible as they are difficult to recover in pure form for reuse.
- US Patent Application No. 20040043967 Al describes the preparation of bisphosphonic acids by using the diluents other than halogenated hydrocarbons, but overall yield of the process is 56% to 80%.
- U.S. Pat. No. 6,562,974 describes the preparation of bisphosphonates in an overall yield of 77% by using phosphorous acid as a reactant/solvent in presence of base. The disadvantage of this process is that the reaction mixture becomes very viscous without a solvent.
- the present inventors have focused on the problems associated with the prior art process and has developed an improved process for the preparation of Minodronic acid.
- present invention provides a process for preparing Minodronic acid or a salt thereof, comprising a step of converting a compound of formula II in to compound of formula III which is further converted in to compound of formula (VI), wherein Rl is a halogen or a carboxy-activating group and R2 is CI -C6 alkoxy, aralkoxy or phenoxy (both optionally substituted with C1C6 alkyl or alkoxy);
- the present invention provides process for producing a bisphosphonic acid compound which process comprises reaction of a carboxylic acid compound or as salt thereof with phosphorous acid and phosphorous trichloride in presence of tetramethylurea as a solvent.
- the present invention provides a purification process for crude Minodronic acid which comprises acid base treatment with crude Minodronic acid.
- the present invention provides a process to obtain pure Minodronic acid using acid base treatment and also provides the process to obtain pure crystal D of Minodronic acid.
- the present inventors have found that employing process of the present invention for the preparation of Minodronic acid, overcomes the drawbacks of the prior art and may be prepared and subsequently converted to Minodronic acid in high yield and purity.
- Fig 1 is the SS-NMR of the crystalline Minodronic acid hydrate before micronisation.
- Fig 2 is the SS-NMR of the crystalline Minodronic acid hydrate after micronisation.
- a further significant advantage of the present invention relates to achievement of purity of the final compound as per JP grade by using simple acid base treatment with high yield.
- present invention provides process where Imidazo [l,2-a]pyridine reacts with oxalyl derivative in presence of suitable solvent.
- Oxalyl derivative may contain on one end any halogen such as CI, Br or I or any leaving group such as mesyl, tosyl , etc at the other end any ester group such as substituted or unsubstituted alkyl, aryl or aralkyl group which can subsequently convert in to acid group.
- Suitable solvents used during reaction are high boiling non polar solvents like toluene, xylene etc. Reaction is carried out at a temperature of 50-150°C, more preferably 100-110°C. The reaction time may between 5 hour to 50 hours, specifically about 20 hours to 30 hours.
- the acylated compound of formula III is isolated from a suitable solvent by conventional methods such as cooling, seeding, partial removal of the solvent from the solution, by adding an anti solvent to the solution, evaporation, vacuum distillation or a combination thereof.
- the reaction mass containing the acylated compound of formula III obtained is concentrated and then taken for next step.
- the base used in step -(b) is selected from the group consisting of sodium hydroxide, potassium hydroxide, lithium hydroxide, cesium hydroxide, magnesium hydroxide, calcium hydroxide, sodium hydride, lithium hydride, potassium hydride, sodamide, lithium amide, potassium amide, sodium methoxide, potassium tert- butoxide, sodium tert-butoxide, sodium tert-pentoxide, lithium tert butoxide.
- hydrolysis of ester group Wolff-Kishner reduction is carried out with hydrazine hydrate in presence of base. Reaction is carried out at reflux temperature and its required long time about 20 to 30 hrs for completion.
- Imidazo [1, 2-a] pyridine-3yl acetic acid is isolated as hydrochloride salt in a pure form which is an important intermediate of Minodronic acid.
- the solvent used in step -(c) is selected from the group consisting of aprotic polar solvents such as ⁇ , ⁇ '-dimethylethyleneurea (DMEU), N,N'-dimethyl propylene urea (DMPU), l-methyl-2-pyrrolidone (NMP), Tetramethylurea (TMU) , acetonitrile or a mixture of two or more thereof.
- aprotic polar solvents such as ⁇ , ⁇ '-dimethylethyleneurea (DMEU), N,N'-dimethyl propylene urea (DMPU), l-methyl-2-pyrrolidone (NMP), Tetramethylurea (TMU) , acetonitrile or a mixture of two or more thereof.
- Minodronic acid The most often used reaction of obtaining Minodronic acid is the reaction of Imidazo [1, 2-a] pyridine-3yl acetic acid with the phosphonating agent such as H 3 PO 3 /PCI 3 or H 3 PO 3 /POCI 3 .
- the number of publications implicates the problem of dense and heterogenic reaction mass that is not even stirrable which is solved by use of suitable solvents.
- the use of those solvents deteriorates the profitability of the process, makes isolation and purification of the product more difficult which results in complication of process implementation into the industrial scale.
- tetramethylurea as a solvent leads to obtaining Minodronic acid and simultaneously ensuring the stirrable reaction mixture. Moreover, such solution allows for simplification of the process to a greater extent, which is advantageous for implementation in to the industrial scale. Additionally, tetramethylurea is a higher boiling, less toxic solvent and can be recoverable solvent.
- Minodronic acid provides end product with more non-polar impurities which is difficult to remove and requires additional purification, thereby not suitable for commercial point of view.
- the same has been avoided by the present invention by simple acid base treatment.
- Minodronic acid hydrate The Three main known impurities of Minodronic acid hydrate are:
- Impurity-II is detected and resolved from Minodronic acid hydrate by HPLC with a relative retention time (hereafter referred as RRT) of 1.64.
- RRT relative retention time
- Impurity-II Imidazo [1,2-a] pyridine-3-yl acetic acid hydrochloride
- Impurity-II is detected and resolved from Minodronic acid hydrate by HPLC with an RRT of 2.77.
- the impurity-III is detected and resolved from Minodronic acid hydrate by HPLC with an RRT of 5.31.
- Minodronic acid obtained from this present invention contain less than 1% particularly less than 0.5% of impurity and more particularly less than 0.1% of total impurity.
- the present invention provides the robust process to eliminate the non polar impurities particularly at 1.28 RRT which is otherwise requires additional purification. Accordingly the present invention provides a purification process for crude Minodronic acid which comprises acid base treatment with crude Minodronic acid.
- process for purifying Minodronic acid involves preparation of disodium salt by stirring wet crude Minodronic acid with NaOH solution at pH 6.5-7.0. Stirring with methanol and then filtering product which removes all impurities in filterate. Pure disodium salt is now converted in to Minodronic acid by refluxing with HC1 solution.
- present invention gives process to prepare only crystal D.
- the method according to this invention involves dissolving Minodronic acid in dilute HC1 solution (4.0 lit.) at reflux temperature and then cooling 70-80°C very slowly. Further allowing it to stir at this temperature for more than 5 hrs at 100-110 RPM and then slowly cooling to room temperature gives pure crystal D.
- Minodronic acid hydrate obtained as per the present invention can be further micronized, milled or sieved to get the desired particle size required for pharmaceutical composition to achieve the desired dissolution profile.
- Example-I preparation of Imidazo[l,2-a]pyridine.
- Tetramethylurea 600 ml
- Phosphorous acid 115.68 g
- process water 30.0ml
- Imidazo [1, 2-a] pyridine-3yl acetic acid hydrochloride 100.0 g
- the LC system used for method development and forced degradation studies and method validation was Waters-Alliance (manufactured by Waters India Ltd) LC system with a photo diode detector. The out put signal was monitored and processed using Empower software system (designed by Waters India) on IBM computer (Digital Equipment Co).
- the chromatographic column used was a waters make X-bridge CI 8 (250 mm x 4.6 mm) with 5.0 ⁇ particles.
- the mobile phase consists 32.3 g of dipotassium hydrogen phosphate anhydrous, 3.0 g of EDTA-disodium salt, and 2.0 g Tetrabutyl ammonium dihydrogen phosphate into a suitable container. Dissolve the contents in 800 mL of water. Pipette out 2.0 mL of concentrated Hydrochloric acid into the same container, dissolve and mix well. Adjust the pH of the solution to 7.5. Transfer the contents to 1000 mL measuring cylinder and dilute to 960 mL with water. Transfer the contents to a suitable container and mix well.
- Reference solution - (a) preparation (Minodronic acid hydrate and each impurity is @ 0.1%).
- Spiked solution (Minodronic acid hydrate + 0.10% of each Known impurity spiked solution) @ 280 nm.
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Abstract
The present invention relates to an improved process for the preparation of Minodronic acid. The present invention particularly relates to a process for the preparation of Minodronic acid from imidazo [1,2-a]pyridine.
Description
TITLE: AN IMPROVED PROCESS FOR PREPARATION OF MINODRONIC ACID FIELD OF THE INVENTION
The present invention relates to an improved, commercially viable and industrially advantageous process for the preparation of Minodronic acid.
BACKGROUND OF THE INVENTION
The bisphosponic acids and their pharmaceutically acceptable salts are an important class of medicaments that act as specific inhibitor of Osteoclast-mediated bone resorption and are useful in the treatment of bone disorders such as Paget's disease and osteoporosis. Bisphosphonates are synthetic analogs of pyrophosphate that bind to the hydroxy-apatite found in the bone.
Minodronic acid is a compound represented by the following formula (I), which has excellent bone resorption inhibitory activity, anti-inflammatory activity and analgesic- antipyretic activity and is useful for the treatment of diseases in which increased bone resorption participates, such as Paget's disease, hypercalcemia, bone metastasis of cancer and osteoporosis, as well as progress in the bone resorption (induction of osteoporosis) caused by inflammatory joint diseases such as rheumatoid arthritis and the like
Few processes for the synthesis of Minodronic acid have been described in the literature. EP0354806 disclose process for preparation of Minodronic acid as below.
C ibes process as below.
CNl 02020676 also disclose process for preparation of Minodronic
The above processes for preparation of Minodronic acid require many numbers of steps and involve unfriendly reagents. The process is less economical, relatively less safe and time-consuming. Hence such technology is not readily suitable for commercial production.
Several methods for making bisphosphonic acids or its pharmaceutically acceptable salts have been disclosed. The synthesis are based on reacting a carboxylic acid with a mixture of phosphorous acid and one of the following phosphorus halides: phosphorus trichloride (PCI3), phosphorus oxychloride (POCI3), phosphorus pentachloride (PCI5), phosphorus tribromide (PBr3), phosphorus oxybromide (POBr3) or phosphorus pentabromide (PBr5), then quenching the reaction mixture with water or a non-oxidizing aqueous acid, followed by heating to hydrolyze the phosphorus intermediates to the final product.
U.S. Pat. No. 4,407,761 describes the synthesis of 4-amino-l-hydroxybutylidene-l ,l - bisphosphonic acid (alendronic acid) and other bisphosphonic acids. The reaction has been carried out in the presence of diluent, e.g. chlorinated hydrocarbons, especially chlorobenzene, which does not solubilize the reaction components and serves only as a heat carrier. The reaction starts as a two-phase system, in which the melted phase gradually thickens into a non-stirrabie mass. This semi solid sticky mass finally turns into a hard, rigid material, thereby coating the walls of the reaction vessel and thus preventing the smooth heat transfer and complicating the product work-up. The overall yield of this process is variable i.e. 45% to 56%. The solvent i.e. chlorobenzene used in the reaction is carcinogenic in nature and thus not recommendable for industrial scale.
U. S. Pat No. 4,922,007 and U.S. Pat. No, 5,019,651 reveal a solution to the solidification. Methanesulfonic acid (MSA) is used to solubilize the reaction components and keep the reaction mixture stirrable up to completion of the reaction. The optimum temperature of phosphonylation reactions using phosphorus trichloride is 90° C. or high. Although the problems with physical characteristics of the reaction appeared solved, a safety problem surfaced. Methanesulfonic acid reacts with phosphorus trichloride and under adiabatic conditions, i .e. above 85° C, the reaction mixture becomes uncontrollably exothermic, which is accompanied by high pressure and, therefore, is not very safe on large-scale production.
US. Pat. No. 5,908,959 employs poly alkylene glycols as reaction solvent for synthesizing bisphosphonates. The use of poly alkylene glycols on industrial scale is not very feasible as they are difficult to recover in pure form for reuse.
US Patent Application No. 20040043967 Al describes the preparation of bisphosphonic acids by using the diluents other than halogenated hydrocarbons, but overall yield of the process is 56% to 80%. On the other hand U.S. Pat. No. 6,562,974 describes the preparation of bisphosphonates in an overall yield of 77% by using phosphorous acid as a reactant/solvent in presence of base. The disadvantage of this process is that the reaction mixture becomes very viscous without a solvent.
Apart from above mentioned process patents; polymorph patents and patent applications are available. US5480875 describes different crystalline forms of Minodronic acid hydrate, Minodronic acid anhydrous and Minodronic acid sodium salt and their process for preparation. This patent describes two Minodronic acid hydrate polymorph as crystal D and crystal E. Both the crystals show similar XRD pattern but having different TG- DSC curves. This patent describes the monohydrate crystals D and E can be produced by dissolving the crystals in aqueous hydrochloric acid solution with specific conditions of cooling and stirring. But, these processes can not reproducibly giving single polymorph crystal D.
Based on the aforementioned drawbacks, prior art processes find to be unsuitable for preparation of Minodronic acid at lab scale and commercial scale operations. Hence, a need still remains for an improved and commercially viable process of preparing pure Minodronic acid that will solve the aforesaid problems associated with process described in the prior art and will be suitable for large-scale preparation, in lesser reaction time, in terms of simplicity, purity and yield of the product.
SUMMARY OF THE INVENTION
The present inventors have focused on the problems associated with the prior art process and has developed an improved process for the preparation of Minodronic acid.
Therefore, in one aspect present invention provides a process for preparing Minodronic acid or a salt thereof, comprising a step of converting a compound of formula II in to compound of formula III which is further converted in to compound of formula (VI),
wherein Rl is a halogen or a carboxy-activating group and R2 is CI -C6 alkoxy, aralkoxy or phenoxy (both optionally substituted with C1C6 alkyl or alkoxy);
In another aspect the present invention provides process for producing a bisphosphonic acid compound which process comprises reaction of a carboxylic acid compound or as salt thereof with phosphorous acid and phosphorous trichloride in presence of tetramethylurea as a solvent.
In another aspect the present invention provides a purification process for crude Minodronic acid which comprises acid base treatment with crude Minodronic acid.
In yet another aspect the present invention provides a process to obtain pure Minodronic acid using acid base treatment and also provides the process to obtain pure crystal D of Minodronic acid.
The present inventors have found that employing process of the present invention for the preparation of Minodronic acid, overcomes the drawbacks of the prior art and may be prepared and subsequently converted to Minodronic acid in high yield and purity.
BRIEF DESCRIPTION OF THE DRAWINGS:
Fig 1 is the SS-NMR of the crystalline Minodronic acid hydrate before micronisation. Fig 2 is the SS-NMR of the crystalline Minodronic acid hydrate after micronisation.
DETAILED DESCRIPTION OF THE INVENTION:
Aspects, advantageous features and preferred embodiments of the present invention will be described in further detail below, noting however that such aspects, advantages features as well as embodiments and examples are presented for illustrative purposes only and shall not limit the invention in any way.
The use of ethyl oxalyl chloride to prepare Minodronic acid intermediate-Imidazo [1, 2-a] pyridine-3yl acetic acid hydrochloride is a significant point of the present invention, which distinguish over every prior art. Use of tetramethylurea as a solvent for phosphorylation is also a crucial point over prior art.
A further significant advantage of the present invention relates to achievement of purity of the final compound as per JP grade by using simple acid base treatment with high yield.
In one embodiment the present invention provides a process for preparation of Minodronic acid of formula I mprising the steps of:
a. reacting a imidazo [1, 2-a] pyridine of formula II with an ethyl oxalyl chloride to obtain compound of formula III):
b. treating compound of formula III with base and hydrazine hydrate to obtain compound of formula VI:
c. reacting the resulting carboxylic acid compound of formula VI with phosphorous acid and a phosphorous chloride selected from PCI3, PCI5 and POCI3, in an aprotic polar solvent.
In one embodiment present invention provides process where Imidazo [l,2-a]pyridine reacts with oxalyl derivative in presence of suitable solvent. Oxalyl derivative may contain on one end any halogen such as CI, Br or I or any leaving group such as mesyl, tosyl , etc at the other end any ester group such as substituted or unsubstituted alkyl, aryl or aralkyl group which can subsequently convert in to acid group. Suitable solvents used during reaction are high boiling non polar solvents like toluene, xylene etc. Reaction is carried out at a temperature of 50-150°C, more preferably 100-110°C. The reaction time may between 5 hour to 50 hours, specifically about 20 hours to 30 hours.
In one embodiment, the acylated compound of formula III is isolated from a suitable solvent by conventional methods such as cooling, seeding, partial removal of the solvent from the solution, by adding an anti solvent to the solution, evaporation, vacuum distillation or a combination thereof. In another embodiment, the reaction mass containing the acylated compound of formula III obtained is concentrated and then taken for next step.
In one embodiment, the base used in step -(b) is selected from the group consisting of sodium hydroxide, potassium hydroxide, lithium hydroxide, cesium hydroxide, magnesium hydroxide, calcium hydroxide, sodium hydride, lithium hydride, potassium hydride, sodamide, lithium amide, potassium amide, sodium methoxide, potassium tert- butoxide, sodium tert-butoxide, sodium tert-pentoxide, lithium tert butoxide. After hydrolysis of ester group Wolff-Kishner reduction is carried out with hydrazine hydrate in presence of base. Reaction is carried out at reflux temperature and its required long time about 20 to 30 hrs for completion.
In another embodiment Imidazo [1, 2-a] pyridine-3yl acetic acid is isolated as hydrochloride salt in a pure form which is an important intermediate of Minodronic acid.
In one embodiment, the solvent used in step -(c) is selected from the group consisting of aprotic polar solvents such as Ν,Ν'-dimethylethyleneurea (DMEU), N,N'-dimethyl
propylene urea (DMPU), l-methyl-2-pyrrolidone (NMP), Tetramethylurea (TMU) , acetonitrile or a mixture of two or more thereof.
The most often used reaction of obtaining Minodronic acid is the reaction of Imidazo [1, 2-a] pyridine-3yl acetic acid with the phosphonating agent such as H3PO3/PCI3 or H3PO3/POCI3. Also for this reaction the number of publications implicates the problem of dense and heterogenic reaction mass that is not even stirrable which is solved by use of suitable solvents. However, the use of those solvents deteriorates the profitability of the process, makes isolation and purification of the product more difficult which results in complication of process implementation into the industrial scale.
Unexpectedly, it was found that the use of tetramethylurea as a solvent leads to obtaining Minodronic acid and simultaneously ensuring the stirrable reaction mixture. Moreover, such solution allows for simplification of the process to a greater extent, which is advantageous for implementation in to the industrial scale. Additionally, tetramethylurea is a higher boiling, less toxic solvent and can be recoverable solvent.
The conventional process of Minodronic acid provides end product with more non-polar impurities which is difficult to remove and requires additional purification, thereby not suitable for commercial point of view. The same has been avoided by the present invention by simple acid base treatment.
The Three main known impurities of Minodronic acid hydrate are:
Impurity-I is detected and resolved from Minodronic acid hydrate by HPLC with a relative retention time (hereafter referred as RRT) of 1.64.
(ii) Imidazo [1,2-a] pyridine-3-yl acetic acid hydrochloride (Impurity-II), which has the following structure:
Impurity-II is detected and resolved from Minodronic acid hydrate by HPLC with an RRT of 2.77.
(iii) Imidazo [1,2-a] pyridine-3-yl (oxo) acetic acid (Impurity-III), which has the following structure:
The impurity-III is detected and resolved from Minodronic acid hydrate by HPLC with an RRT of 5.31.
(iv) An unknown impurity at 1.28 RRT, which m/z found to be as 291 [M+H] by LC-
MS/MS.
The Unknown impurity at 1.28 RRT is detected and resolved from Minodronic acid hydrate by HPLC with an RRT of 1.28.
Surprisingly the Minodronic acid obtained from this present invention contain less than 1% particularly less than 0.5% of impurity and more particularly less than 0.1% of total impurity.
No prior art teaches or motivates the invention provided in the present application, and constitute novelty of the present invention. Thus the present invention provides the robust process to eliminate the non polar impurities particularly at 1.28 RRT which is otherwise
requires additional purification. Accordingly the present invention provides a purification process for crude Minodronic acid which comprises acid base treatment with crude Minodronic acid.
In another embodiment process for purifying Minodronic acid involves preparation of disodium salt by stirring wet crude Minodronic acid with NaOH solution at pH 6.5-7.0. Stirring with methanol and then filtering product which removes all impurities in filterate. Pure disodium salt is now converted in to Minodronic acid by refluxing with HC1 solution.
In yet another embodiment present invention gives process to prepare only crystal D. The method according to this invention involves dissolving Minodronic acid in dilute HC1 solution (4.0 lit.) at reflux temperature and then cooling 70-80°C very slowly. Further allowing it to stir at this temperature for more than 5 hrs at 100-110 RPM and then slowly cooling to room temperature gives pure crystal D.
Further Minodronic acid hydrate obtained as per the present invention can be further micronized, milled or sieved to get the desired particle size required for pharmaceutical composition to achieve the desired dissolution profile.
The main embodiment of the present invention is described in the scheme I.
The invention will be further described with reference to the following non limiting examples.
Example-I : preparation of Imidazo[l,2-a]pyridine.
Charged 2-Aminopyridine (100.00 g), Dichloromethane (1000ml) and sodium bicarbonate (98.17 g) into the reactor. Slowly added Chloroacetaldehyde aqueous solution (205.85 g, Assay: 48.58%) into reaction mixture at temperature between 10- 25°C. Stirred the reaction mixture for 6 hours at room temperature. After completion of the reaction, charged process water (300 ml) into reaction mixture and extracted product in dichloromethane. Removed dichloromethane atmospherically below 60°C and then distilled product under vacuum, (yield-75%)
Example-II: Imidazo [1, 2-a] pyridine-3yl acetic acid hydrochloride
Charged toluene, Imidazo [l,2-a]pyridine (100.0 g) and Ethyl oxalyl chloride (288.91 g) in to the reactor. Stirred the reaction mixture for 24 hours at 113±3°C. After completion of the reaction, distilled off toluene under vacuum below 80°C and then charged water (800 ml) into reaction mass. Cooled the reaction mass to room temperature and then adjusted the reaction mass pH between 6.5 and 7.5 by using aqueous potassium hydroxide solution (-300 ml, 30%). Charged Potassium hydroxide (71.20 g) into the reaction mass and heated the reaction mixture for 2 hrs at 52±3°C. After completion of the reaction, distilled off solvent and added hydrazine hydrate (63.5 g, 80 % solution in water) into reaction mass. Stirred the reaction mixture for 24 hours at 102±2°C. After completion of reaction, adjusted the reaction mixture pH between 10.5-11.0 by using cone. HC1 solution (-200 ml). Charged activated charcoal (10.0 g) and stirred the reaction mixture for 30 mins. Filtered the reaction mixture through hyflo bed; washed the hyflo bed with process water (200 ml). Adjusted the reaction mixture pH 6.0-6.5 with cone. HC1 solution (-100 ml). Filtered the reaction mixture and washed the wet cake with acetone in centrifuge bag (200 ml). Charged ethyl acetate.HCl [(8%), 464 g) and Ethyl acetate (200 ml) and wet cake in to the reactor. Heated the reaction mixture to 53±2°C. Stirred the reaction mixture for 4 hours at 53±2°C. Cooled the reaction mixture to 3±2°C. Filtered the reaction mixture and washed with chilled ethyl acetate (200 ml). Dried the material under vacuum for 8 hours at 53±2°C. (Yield:75.00%)
Example-Ill: Preparation of l-Hydroxy-2-(Imidazo [1, 2-a] pyridine-3-yl) ethane-1, 1-bis (phosphonic acid) monohydrate
Charged Tetramethylurea (600 ml), Phosphorous acid (115.68 g) and process water (30.0ml) in to the reactor. Charged Imidazo [1, 2-a] pyridine-3yl acetic acid hydrochloride (100.0 g) into reaction mass and stirred the reaction mass for 15 minutes. Cooled the reaction mass to 17±3°C and added Phosphorous trichloride (290.64g) into the reaction mass below 40°C. Heated the reaction mixture to 63±3°C and stirred the reaction mixture for 3 hours. After completion of reaction, distilled off phosphorous trichloride under vacuum. Cooled the residual mass to room temperature and added 20-24 % aqueous HC1 solution (800 ml) to the reaction mixture below temperature 80°C. Stirred the reaction mixture for 4 hours at 95±2°C. Cooled the reaction mixture to 75±2°C and charged toluene (500 ml) into the reaction mixture. Stirred the reaction mixture for 30 minutes at 75±2°C. Separated the lower aqueous layer. Charged aqueous layer into another reactor and cooled the reaction mass to room temperature. Adjusted the reaction mass pH between 1.1-1.8 by using aqueous sodium hydroxide solution. (30%, ~ 1200 ml) below 30±5°C to precipitate the product. Stir the reaction mixture for 3 hours. Filtered the product and washed with water. Charged wet cake into water and adjusted the reaction mixture pH between 6.5-7.0 by using aqueous sodium hydroxide solution (-150 ml, 30%). Added methanol (15 vol.) into the reaction mass slowly at 23±3°C and stirred the reaction mixture for 3 hours. Filtered the reaction mixture and wash the wet cake with methanol. Charged 7.0-8.0 % HC1 solution and the wet cake in another reactor. Heated the reaction mixture to 95±2°C for 60 minutes. Cooled the reaction mixture to 13°C and stirred the reaction mixture for 4hrs. Filtered the reaction mixture and washed with water. Dried the material for 8 hours at 50-55°C under reduced pressure. (Yield: 73.00%)
Example~IV : Preparation of l-Hydroxy-2-(Imidazo [1, 2-a] pyridine-3-yl) ethane- 1, 1-bis (phosphonic acid) monohydrate
Charged 3.5-4.0 % HC1 solution (4.0 lit.) and stage-Ill (100.0 g) into the reactor and heated the reaction mixture to 99°C for 20 minutes to get the clear solution. Filtered hot the reaction mixture through cartridge filter and then allowed to cool the reaction mixture
to 77±2°C at a cooling rate of 1°C / 5 minutes at 100-110 RPM. Stirred the reaction mixture at 77±2°C for 5 hrs at 100-110 RPM. Cooled the reaction mixture to 27±3°C at a cooling rate of 2°C / 5 minutes at 100-110 RPM. Stirred the reaction mixture for 3 hrs at 27±3°C at 100-110 RPM. Filtered the reaction mixture and washed the wet cake with process water (100 ml) at 27±3°C. Dried the material for 8 hours at 53±2°C under reduced pressure, (yield 90.00%)
Experimental
The LC system, used for method development and forced degradation studies and method validation was Waters-Alliance (manufactured by Waters India Ltd) LC system with a photo diode detector. The out put signal was monitored and processed using Empower software system (designed by Waters India) on IBM computer (Digital Equipment Co).
The chromatographic column used was a waters make X-bridge CI 8 (250 mm x 4.6 mm) with 5.0μηι particles. The mobile phase consists 32.3 g of dipotassium hydrogen phosphate anhydrous, 3.0 g of EDTA-disodium salt, and 2.0 g Tetrabutyl ammonium dihydrogen phosphate into a suitable container. Dissolve the contents in 800 mL of water. Pipette out 2.0 mL of concentrated Hydrochloric acid into the same container, dissolve and mix well. Adjust the pH of the solution to 7.5. Transfer the contents to 1000 mL measuring cylinder and dilute to 960 mL with water. Transfer the contents to a suitable container and mix well. Add 40 mL of methanol into the same container and mix. The flow rate of the mobile phase was kept at 1.0 ml/min. beginning with the Isocratic elutions. The column temperature was maintained at 35°C and the wavelength was monitored at a wavelength of 280 nm. The injection volume was 10 μΕ for related substances determination. Mobile phase was used as diluent during the standard and test samples preparation.
Preparation of impurity stock solution:
Weigh and transfer about 5 mg each of Impurity-I, Impurity-II and Impurity-III standards into a 100 mL volumetric flask, add about 30 mL diluent and sonicate to dissolve. Make up the volume with diluent and mix well.
Intermediate Standard stock preparation.
Weigh and transfer about 50 ± 5 mg of Minodronic acid hydrate reference standard into a 25 mL volumetric flask. Add about 10 mL diluent into the same volumetric flask and sonicate to dissolve. Make up to the volume with diluent and mix well.
Standard stock preparation
Pipette out 1.0 mL of the above solution to 100 mL volumetric flask and make up to the mark with diluent.
Reference solution - (a) preparation (Minodronic acid hydrate and each impurity is @ 0.1%).
Pipette out of 5.0 mL of standard stock preparation and 2.0 mL of Impurity stock solution into a 50 mL volumetric flask. Make up to the volume with diluent and mix well.
Reference solution-(b) preparation (Minodronic acid hydrate @ 0.1%):
Pipette out 5.0 mL of standard stock preparation into a 50 mL volumetric flask and make up the volume with diluent and mix well.
Spiked solution (Minodronic acid hydrate + 0.10% of each Known impurity spiked solution) @ 280 nm.
Sample solution (Minodronic acid+ Unknown impurity at 1.28 RRT) @ 280 nm.
Claims
Claims:
1. A process for preparing Minodronic acid compound of formula (I) or a salt thereof,
which process comprises reacting a compound of formula II with a compound of formula V to form compound of formula III
wherein, Ri is a halogen or a carboxy-activating group, R2 is Ci -C6 alkoxy, aralkoxy or phenoxy (both optionally substituted with Ci-C6 alkyl or alkoxy). A process for preparing Minodronic acid compound of formula (I) or a salt thereof,
which process comprises converting a compound of formula III in to compound of formula VI
inwhich R2 is Ci -C6 alkoxy, aralkoxy or phenoxy (both optionally substituted with Ci-C6 alkyl or alkoxy).
3. A process for producing a bisphosphonic acid compound which process comprises reaction of a carboxylic acid compound or as salt thereof with phosphorous acid and a phosphorous chloride selected from PCI3, PCI5 and POCI3 in presence of tetramethylurea.
4. A process for producing a Minodronic acid which process comprises reaction of a carboxylic acid compound of formula (VI) with phosphorous acid and phosphorous trichloride in presence of tetramethylurea.
a. reacting a imidazo [1, 2-a] pyridine of formula II with an ethyl oxalyl chloride to obtain compound of formula III):
b. treating compound of formula III with base and hydrazine hydrate to obtain compound of formula VI:
c. reacting the resulting carboxylic acid compound of formula VI with phosphorous acid and a phosphorous chloride selected from PCI3, PCI5 and POCI3 in an aprotic polar solvent.
6. A process according to claim 5, wherein the base used in step-(b) is selected from alkali metal hydroxide, carbonates, alkaline earth hydroxides or carbonates, alkoxide bases; preferably potassium hydroxide.
7. A process according to claim 5, wherein an aprotic polar solvent used in step-(c) is selected from Ν,Ν'-dimethylethyleneurea (DMEU), Ν,Ν'- dimethylpropyleneurea (DMPU), l-methyl-2-pyrrolidone (NMP), Tetramethylurea (TMU) , acetonitrile or a mixture of two or more thereof.
8. A process for purifying Minodronic acid of formula I comprising the steps of: a. stirring the Minodronic acid in water;
b. adjusting the pH more than 6.0 using aqueous NaOH solution;
c. adding methanol in the reaction mixture;
d. filtering the product;
e. mixing the product with dil. HC1 solution;
f. heating the reaction mixture to 80-95°C;
g. cooling the reaction mixture below 20°C;
h. filtering the product and washing with water;
i. optionally recrystallising above Minodronic acid using suitable solvent system.
9. A process for preparing pure crystal D of Minodronic acid comprising the steps of:
a. dissolving Minodronic acid in dilute HC1 solution (4.0 lit.) at reflux temperature;
b. optionally filtering the hot solution;
c. slowly cooling the reaction mixture at 70-80°C;
d. stirring the reaction mixture at 70-80°C for more than 5 hrs at 100-110 RPM; e. slowly cooling the reaction mixture to room temperature;
f. filtering the product and washing with water;
g. drying the material under vacuum.
10. A process for producing a Minodronic acid which process comprises reaction of a carboxylic acid compound of formula (VI) with phosphorous acid in presence of tetramethylurea where addition of Phosphorous trichloride into the reaction mass below 40°C.
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104497048A (en) * | 2014-12-10 | 2015-04-08 | 哈药集团技术中心 | Preparation method of minodronic acid |
WO2016024287A1 (en) * | 2014-08-14 | 2016-02-18 | Ind-Swift Laboratories Limited | An improved process for preparing pure minodronic acid and intermediates thereof |
CN108690019A (en) * | 2018-07-04 | 2018-10-23 | 南京拉艾夫医药科技有限公司 | Minodronate intermediate synthesis system and its synthesis technology |
CN114384186A (en) * | 2022-01-21 | 2022-04-22 | 王立强 | Method for measuring content of (2E, 4E) -ethyl-4- (pyridine-2-yl imino) -2-ethyl crotonate |
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CN107033187A (en) * | 2017-04-28 | 2017-08-11 | 江苏笃诚医药科技股份有限公司 | A kind of preparation method of minodronic acid |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002014306A1 (en) * | 2000-08-17 | 2002-02-21 | Cilag Ag | Process for the preparation of imidazopyridines |
WO2007023504A1 (en) * | 2005-08-24 | 2007-03-01 | Elder Pharmaceuticals Ltd | Process for the preparation of imidazopyridines |
-
2013
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Patent Citations (2)
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WO2002014306A1 (en) * | 2000-08-17 | 2002-02-21 | Cilag Ag | Process for the preparation of imidazopyridines |
WO2007023504A1 (en) * | 2005-08-24 | 2007-03-01 | Elder Pharmaceuticals Ltd | Process for the preparation of imidazopyridines |
Non-Patent Citations (1)
Title |
---|
DIVVELA V. N. SRINIVASA RAO ET AL.: 'facile one pot synthesis of bisphosphonic acids and their sodium salts from nitriles' ARKIVOC vol. XIV, 2007, pages 34 - 38 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2016024287A1 (en) * | 2014-08-14 | 2016-02-18 | Ind-Swift Laboratories Limited | An improved process for preparing pure minodronic acid and intermediates thereof |
CN104497048A (en) * | 2014-12-10 | 2015-04-08 | 哈药集团技术中心 | Preparation method of minodronic acid |
CN108690019A (en) * | 2018-07-04 | 2018-10-23 | 南京拉艾夫医药科技有限公司 | Minodronate intermediate synthesis system and its synthesis technology |
CN114384186A (en) * | 2022-01-21 | 2022-04-22 | 王立强 | Method for measuring content of (2E, 4E) -ethyl-4- (pyridine-2-yl imino) -2-ethyl crotonate |
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IN2012MU03517A (en) | 2015-06-26 |
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