WO2014077300A1

WO2014077300A1 - Antibacterial liquid composition

Info

Publication number: WO2014077300A1
Application number: PCT/JP2013/080744
Authority: WO
Inventors: 博朗飯田; 雅友水野
Original assignee: イーダ株式会社
Priority date: 2012-11-15
Filing date: 2013-11-14
Publication date: 2014-05-22
Also published as: CN104902758A; JP5318272B1; JP2014097960A

Abstract

[Problem] To make it possible to demonstrate a more stable and excellent antibacterial effect by further enhancing the dispersive characteristics of antibacterial ingredients in an antibacterial composition dually provided with a high level of safety and excellent antibacterial characteristics. [Solution] An antibacterial liquid composition dually provided with a high level of safety and excellent antibacterial characteristics by containing as the ingredients of the antibacterial liquid composition: a water-insoluble metal salt generated by reacting a surfactant and metal ions having antibacterial characteristics; at least one dispersant selected from the group consisting of polyglycerol fatty acid ester, polyoxyethylene (hydrogenated) castor oil, and polyoxyethylene polyoxypropylene glycol; and alcohol, wherein the dispersive characteristics of antibacterial ingredients are enhanced.

Description

Antibacterial liquid composition

The present invention relates to an antibacterial liquid composition having an antibacterial action against bacteria and viruses.

An antibacterial agent is used in various fields for the purpose of preventing contamination and rot by microorganisms, and preventing and treating infection by bacteria or viruses. In general, antibacterial components include concentrated hydrochloric acids, alcohols, phenols, halogens, formaldehyde, glutaraldehyde, higher fatty acids, lower fatty acids, lactones, (amphoteric or anionic) surfactants, silver and copper There are various compounds such as ozone / hydrogen peroxide, peracetic acid, sodium hypochlorite and the like.

Here, from the viewpoint of safety to the human body, the Food Sanitation Law stipulates antibacterial components that can be used for cleaning, antibacterial, and sterilization of food in the food service industry and food factories. Examples of such antibacterial components include sodium hypochlorite, organic acids such as acetic acid, lactic acid, citric acid and fumaric acid, metal ions having antibacterial activity such as zinc, copper, iron and silver, and polyhydric alcohols. And nonionic surfactants such as sorbitan fatty acid esters and glycerin fatty acid esters are recognized.

Among these antibacterial components, sodium hypochlorite has disadvantages such as practical safety (careful not to mix with acid, etc.), loss of sterilization effect due to the presence of organic substances, strong odor, etc. Some may not be usable. Some organic acids have a strong odor similar to sodium hypochlorite.

On the other hand, an antibacterial agent composition containing a polyhydric alcohol antibacterial agent and a silver antibacterial agent has been proposed from the viewpoints of safety, prevention of sterilization effect loss, odor suppression, and the like (for example, Patent Documents). 1). In the antibacterial agent composition described in Patent Document 1, there are certainly no inconveniences such as attention to practical safety, loss of sterilization effect, strong odor, etc., but the antibacterial activity is sodium hypochlorite, etc. The antibacterial effect may be insufficient depending on the type and amount of bacteria.

Under such circumstances, the present inventors have already proposed an antibacterial liquid composition comprising a metal ion having an antibacterial action and a surfactant that forms the metal ion and a metal salt insoluble in water. (See Patent Document 2). As described in this specification, the antibacterial liquid composition described in Patent Document 2 uses only ingredients recognized as food additives, and is water-insoluble formed by these ingredients. Since the metal salt has a high antibacterial activity, it has both high safety and effective antibacterial properties against a wide range of microorganisms.

JP 2012-56854 A JP2011-168518A

However, in the antibacterial liquid composition described in Patent Document 2, depending on the production conditions and storage conditions, the water-insoluble metal salt dispersed in the liquid aggregates, and the aggregate floats in the liquid and is liquid. Sometimes became cloudy or precipitated. In this way, when the water-insoluble metal salt aggregates, the antibacterial effect is reduced and the aggregate adheres to the food compared to the case where the water-insoluble metal salt fine particles are uniformly dispersed in the liquid. There was a fear. Therefore, an antibacterial liquid composition in which aggregation of fine particles of a water-insoluble metal salt is suppressed and dispersibility is further improved has been demanded.

Therefore, the present invention has been made in view of the above circumstances, and in an antibacterial liquid composition that combines high safety and excellent antibacterial properties, by further increasing the dispersibility of antibacterial components, it is more stable and excellent. The purpose is to be able to exert antibacterial effects.

As a result of intensive studies to solve the above-mentioned problems, the present inventors have used a water-insoluble metal salt produced by a reaction between a metal ion having antibacterial properties and a surfactant as an antibacterial component, By using a specific type of dispersant as a dispersant and further using alcohol as a dispersion aid to improve dispersibility, the antibacterial component has high dispersibility in antibacterial compositions having both high safety and excellent antibacterial properties. Based on this finding, the present invention has been completed.

That is, the present invention relates to a water-insoluble metal salt produced by a reaction between a metal ion having antibacterial properties and a surfactant, polyglycerin fatty acid ester, polyoxyethylene (hardened) castor oil, and polyoxyethylene polyoxypropylene glycol. An antibacterial agent containing at least one dispersant selected from the group consisting of alcohol and an alcohol content of 3% by mass to 80% by mass based on the mass of the entire composition. It is a liquid composition.
Here, the metal ions having antibacterial properties are preferably one or more ions selected from the group consisting of aluminum ions, zinc ions, copper ions, iron ions and silver ions.
Moreover, it is especially preferable that the metal ions having antibacterial properties are aluminum ions.
Moreover, it is preferable that the said surfactant is 1 type, or 2 or more types of nonionic surfactant selected from the group which consists of glycerol fatty acid ester, sorbitan fatty acid ester, and sucrose fatty acid ester.
Moreover, it is preferable that the quantity of the said surfactant with respect to the said aluminum ion is 0.4 or more and 15 or less by mass ratio.
Moreover, it is preferable that the said dispersing agent is polyglyceryl fatty acid ester.
Moreover, it is preferable that content of the said dispersing agent is 0.001 mass% or more and 0.3 mass% or less on the basis of the mass of all the compositions.
The alcohol is preferably a lower alcohol having 3 or less carbon atoms.

According to the present invention, in an antibacterial liquid composition having both high safety and excellent antibacterial properties, it is possible to more stably exhibit an excellent antibacterial effect by further increasing the dispersibility of the antibacterial component. .

Hereinafter, preferred embodiments of the present invention will be described in detail.

The antibacterial liquid composition according to the present invention will be described in the following order.
DESCRIPTION OF SYMBOLS 1 Composition of antibacterial liquid composition 2 Manufacturing method of antibacterial liquid composition 3 Principle of antibacterial action 4 Use of antibacterial liquid composition 5 Usage of antibacterial liquid composition

≪Composition of antibacterial liquid composition≫
The antibacterial liquid composition according to the present invention (hereinafter referred to as “the present antibacterial liquid composition”) includes a water-insoluble metal salt produced by a reaction between an antibacterial metal ion and a surfactant, It mainly contains at least one dispersant selected from the group consisting of glycerin fatty acid ester, polyoxyethylene (cured) castor oil, and polyoxyethylene polyoxypropylene glycol, and a dispersion medium. The antibacterial liquid composition is a dispersion aqueous solution (colloidal solution) in which a water-insoluble metal salt is uniformly dispersed in a dispersion medium by a dispersant such as polyglycerin fatty acid ester. Hereinafter, each component will be described in order.

<Water-insoluble metal salt>
In the antibacterial liquid composition, the water-insoluble metal salt is a water-insoluble metal salt formed by a reaction between a metal ion having antibacterial properties and a surfactant. For example, the surfactant is a fatty acid derivative. In this case, a metal soap containing a metal having antibacterial properties is obtained. This water-insoluble metal salt has a role as an antibacterial component in the present antibacterial liquid composition. The antibacterial activity of the aqueous dispersion containing the water-insoluble metal salt, that is, the antibacterial liquid composition is higher than that of the aqueous solution containing a metal ion having antibacterial properties.

Here, the antibacterial liquid composition is usually not preferred in the field of antibacterial agents and cleaning agents, as described in, for example, JP-A-7-29297 and JP-A-2008-214621. The salted-out product, that is, the production of water-insoluble metal salt and the production conditions thereof are actively utilized. In other words, when a surfactant is used, metal ions generate metal soap (water-insoluble metal salt) and cause a reduction in surface activity, so that the use of metal soap has been avoided until now. However, in the present invention, a dispersion solution of a water-insoluble metal salt having an antibacterial activity higher than that of a metal ion having a conventional antibacterial property (the present antibacterial liquid) Composition).

In addition, since the water-insoluble metal salt according to the present invention is hardly decomposed by an acid or alkali, the quality of the antibacterial liquid composition in which the metal salt is dispersed in a solvent is stable for a long time.

(Metallic ions with antibacterial properties)
As described above, although certain metal ions have antibacterial activity per se, the present antibacterial liquid composition is used for food, tableware, and the like due to the antibacterial activity of the water-insoluble metal salt to which the metal ions having antibacterial properties are bound. It is possible to inactivate bacteria, viruses and the like attached to food production apparatuses and skin.

Here, examples of metal ions having antibacterial properties used in the present antibacterial liquid composition include aluminum (Al) ions, zinc (Zn) ions, copper (Cu) ions, iron (Fe) ions, and silver (Ag). Ions can be used. Since these ion sulfates and other inorganic acid salts (for example, zinc sulfate, alum, etc.), gluconates and other organic acid salts are recognized as food additives, the antibacterial liquid composition is used as a food. When used as an antibacterial agent against the above, it is preferable to use the above metal ions. Further, Al ions are particularly preferable as the metal ions having antibacterial properties according to the present invention.

(Surfactant)
The surfactant used in the antibacterial liquid composition is not particularly limited as long as it can generate a water-insoluble metal salt by reacting with the metal ions having antibacterial properties. Any of a surfactant, an amphoteric surfactant, and a nonionic surfactant may be used.

The anionic surfactant used in the antibacterial liquid composition is not particularly limited, and examples thereof include alkyl sulfates, polyoxyethylene alkyl ether sulfates, sulfosuccinates, N-acyl amino acid salts, carboxylates, Examples thereof include sulfonates.

The cationic surfactant used in the antibacterial liquid composition is not particularly limited, and examples thereof include alkyl ammonium salts and quaternary ammonium salts.

The amphoteric surfactant used in the antibacterial liquid composition is not particularly limited, and examples thereof include alkyl (amido) betaine and alkyldimethylamine oxide.

The nonionic surfactant used in the antibacterial liquid composition is not particularly limited. For example, glycerin fatty acid ester, sorbitan fatty acid ester, sucrose fatty acid ester, polyoxyalkylene lauryl ether (for example, polyoxyethylene lauryl ether) ), Polyoxyethylene oleyl cetyl ether, polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, alkyl alkanolamide, alkyl polyglucoxide and the like.

Here, when the antibacterial liquid composition is used for food cleaning, antibacterial and the like, from the viewpoint of safety to the human body, it is a nonionic surfactant that is recognized as a food additive as a surfactant. It is preferable to use an agent. Examples of such nonionic surfactants include glycerin fatty acid esters (monoesters, diesters, and triesters), polyhydric alcohols such as sorbitan fatty acid esters, sucrose fatty acid esters, sugar alcohols, and sugars. Examples include esters with fatty acids. These fatty acid esters may be used alone or in combination of two or more. In addition, since sucrose fatty acid ester has solubilization power with respect to water rather than glycerol fatty acid ester and sorbitan fatty acid ester, the water-insoluble metal salt produced | generated becomes comparatively easy to disperse | distribute in water. Therefore, when importance is attached to the dispersibility of the water-insoluble metal salt, it is preferable to contain a sucrose fatty acid ester. On the other hand, when importance is attached to the balance between the dispersibility of the water-insoluble metal salt and the antibacterial activity, it is preferable to contain a sorbitan fatty acid ester or a glycerin fatty acid ester.

The content of the surfactant in the antibacterial liquid composition is preferably 0.4 or more and 15 or less, and more preferably 1.5 or more and 11 or less, in terms of mass ratio with respect to metal ions having antibacterial properties. More preferably, it is 2 or more and 11 or less. When the surfactant content is within this range, aggregation of water-insoluble metal salt particles in the antibacterial liquid composition can be suppressed, and as a result, dispersibility of the water-insoluble metal salt can be improved. . Therefore, it can suppress that this antibacterial liquid composition becomes cloudy, or the aggregation particle | grains of a water-insoluble metal salt precipitate.

<Dispersant>
In the antibacterial liquid composition, the dispersant has a role of uniformly dispersing the above-described water-insoluble metal salt containing metal ions having antibacterial properties in a dispersion medium described later. Examples of such a dispersant include polyglycerin fatty acid ester, polyoxyethylene (cured) castor oil, and polyoxyethylene polyoxypropylene glycol. These dispersants may be used alone. You may use combining more than a seed. As the dispersant used in the antibacterial liquid composition, it is particularly preferable to use a polyglycerol fatty acid ester. Since polyglycerin fatty acid ester is recognized as a food additive, by using glycerin fatty acid ester, sorbitan fatty acid ester, sucrose fatty acid ester and the like that are recognized as food additives as a surfactant, an antibacterial component ( Both the water-insoluble metal salt) and the dispersant (polyglycerin fatty acid ester) for dispersing the same can be composed of only components that are recognized as food additives. Therefore, the safety of the antibacterial liquid composition to the human body can be increased, and the antibacterial liquid composition can be applied to foods.

Here, the polyglycerin fatty acid ester is generally a fatty acid esterified to one or more hydroxy groups of polyglycerin obtained by dehydrating condensation polymerization of glycerin, and is hydrophilic depending on the degree of polymerization and the number and type of fatty acids. Various types are available, such as sexual and hydrophobic. As the polyglycerol fatty acid ester used in the present antibacterial liquid composition, those capable of satisfactorily dispersing the above-mentioned water-insoluble metal salt are preferable.

Such a polyglycerin fatty acid ester is not particularly limited, but preferably has a degree of polymerization of 8 or more and 12 or less and an acid value of 5 or less. Examples of the fatty acid used for the synthesis of the polyglycerol fatty acid ester used in the antibacterial liquid composition include caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid, stearic acid, and oleic acid. It is done. These fatty acids may be used individually by 1 type, and may be used in combination of 2 or more type.

Further, the content of the dispersant in the antibacterial liquid composition is preferably 0.001% by mass or more and 0.3% by mass or less, and 0.005% by mass or more based on the mass of the entire composition. The content is more preferably 0.2% by mass or less, and still more preferably 0.01% by mass or more and 0.1% by mass or less. When the content of the dispersant is within this range, aggregation of water-insoluble metal salt particles in the antibacterial liquid composition can be suppressed, and as a result, dispersibility of the water-insoluble metal salt can be enhanced. Therefore, it can suppress that this antibacterial liquid composition becomes cloudy, or the aggregation particle | grains of a water-insoluble metal salt precipitate. On the other hand, if the content of the dispersant is too small, the effect of dispersing the water-insoluble metal salt may not be sufficiently exhibited. Conversely, if the content is too large, the antibacterial activity of the antibacterial liquid composition may be reduced.

<Dispersion medium>
The antibacterial liquid composition mainly contains alcohol and water as a dispersion medium in which the above-described water-insoluble metal salt is dispersed. Hereinafter, these components will be described in more detail.

(alcohol)
Alcohol has a role as a dispersing agent together with water, a role as a dispersion aid for improving the dispersibility of the water-insoluble metal salt, and a role as an antibacterial or sterilizing component. In the antibacterial composition, alcohol is an essential component in order to disperse the water-insoluble metal salt particles uniformly without agglomeration.

Here, as the alcohol used in the antibacterial liquid composition, it is preferable to use a lower alcohol having 3 or less carbon atoms. By using such a lower alcohol, the dispersibility of the water-insoluble metal salt can be improved to increase the transparency of the antibacterial liquid composition, and the antibacterial or sterilizing effect against bacteria and viruses can be increased. In addition, when a higher alcohol having 4 or more carbon atoms is used, it becomes difficult to handle because it becomes difficult to dissolve in water and the viscosity increases.

Examples of the lower alcohol described above include methanol, ethanol, 1-propanol, 2-propanol, etc. Of these, ethanol is particularly used from the viewpoint of enhancing safety and antibacterial / sterilizing effects. It is preferable.

The content of alcohol in the antibacterial liquid composition is preferably 3% by mass or more and 80% by mass or less, and more preferably 20% by mass or more and 70% by mass or less, based on the mass of the entire composition. Preferably, it is 40 mass% or more and 60 mass% or less. When the alcohol content is within this range, aggregation of water-insoluble metal salt particles in the antibacterial liquid composition can be suppressed, and as a result, dispersibility of the water-insoluble metal salt can be enhanced. Therefore, it can suppress that this antibacterial liquid composition becomes cloudy, or the aggregation particle | grains of a water-insoluble metal salt precipitate. Moreover, the antibacterial power of this antibacterial liquid composition can be improved more as content of alcohol is the said range.

(water)
Water has a role as a dispersion medium for dispersing the above-described water-insoluble metal salt. As water used in the antibacterial liquid composition, it is preferable to use ion-exchanged water or distilled water. For example, when water containing various metal ions such as tap water is used as water to disperse the water-insoluble metal salt, these metal ions generate salts with the above-described surfactants, dispersants, and the like. There is a possibility that the antibacterial property and liquid stability (maintaining a state in which no precipitate is generated or cloudiness is generated) of the antibacterial liquid composition may be impaired.

The content of water in the antibacterial liquid composition is not particularly limited, and from the viewpoint of achieving both high dispersibility of the water-insoluble metal salt and high antibacterial activity as a whole composition, the balance of other necessary components Should be water.

<Other ingredients>
As described above, the antibacterial liquid composition mainly comprises a water-insoluble metal salt produced by an antibacterial metal ion and a surfactant, a polyglycerin fatty acid ester, an alcohol, and water. In addition, other components as described below may be further contained.

(PH adjuster)
The antibacterial liquid composition may further contain a pH adjuster. The pH adjuster is used to make the antibacterial liquid composition weakly acidic to neutral. By setting the antibacterial composition in a weakly acidic to neutral range, the dispersion stability of the water-insoluble metal salt described above can be improved. From the viewpoint of improving the dispersion stability of the water-insoluble metal salt, the pH of the antibacterial liquid composition is preferably in the range of 3 to 7, more preferably in the range of 3 to 5.

Examples of the pH adjuster used in the antibacterial liquid composition include sodium lactate, sodium citrate, sodium lactate, potassium tartrate, sodium tartrate, sodium malate, sodium fumarate, and sodium succinate. , Other organic acid salts, arginine, sodium carbonate and the like, and those that lower the pH, such as lactic acid, citric acid, phosphoric acid, acetic acid, tartaric acid, malic acid, fumaric acid, adipic acid, other organic acids, glycine, etc. Is mentioned. What is necessary is just to select suitably the kind of pH adjuster used for this antibacterial liquid composition so that the role of the other component contained in this antibacterial liquid composition may not be inhibited.

Further, the content of the pH adjusting agent in the antibacterial liquid composition is not particularly limited, and the composition as a whole is weakly acidic to neutral depending on the type of components contained in the antibacterial liquid composition. An amount that falls within the range may be used.

(Other additives)
In addition, the antibacterial liquid composition may further contain various additives as long as the effects of the present invention described above are not impaired. Examples of such additives include fragrances, essential oils, viscosity modifiers, foam modifiers, enzymes (for degrading proteins, lipids, carbohydrates, etc.), saccharides (sorbitol, xylitol, trehalose, etc.), amino acids, etc. Is mentioned. Examples of the fragrances and essential oils include geraniol, citronellol, eugenol, linalool, terpineol, thymol, menthol, limonene, and perillaaldehyde that are recognized as food additives.

≪Method for producing antibacterial liquid composition≫
As mentioned above, although the composition of this antibacterial liquid composition was demonstrated in detail, it continues and the manufacturing method of this antibacterial liquid composition which has the composition mentioned above is demonstrated. The antibacterial liquid composition mainly includes an inorganic acid salt of a metal that is a source of metal ions having antibacterial properties, a surfactant, a dispersant such as a polyglycerin fatty acid ester, and an alcohol. It can be obtained by mixing with water and adjusting the pH. Specifically, the antibacterial liquid composition can be prepared, for example, as follows.

First, the above-described metal salt containing metal ions having antibacterial properties is dissolved in water (preferably ion-exchanged water). At this time, for example, zinc sulfate, alum (potassium aluminum sulfate, AlK (SO ₄ ) ₂ · 12H ₂ O), copper gluconate, or the like can be used as the metal salt. Next, the above-mentioned surfactant, a dispersant such as polyglycerin fatty acid ester, and alcohol are added to the obtained aqueous solution, and after stirring and mixing, water is further added as necessary. Finally, the antibacterial liquid composition, which is a colloidal dispersion in which a water-insoluble metal salt is dispersed in water, can be obtained by using the pH adjuster described above to adjust the liquidity to an acidic to neutral range. it can.

Thus, when manufacturing this antibacterial liquid composition, by adding a dispersant such as polyglycerin fatty acid ester and alcohol, the aggregation of the water-insoluble metal salt in the antibacterial liquid composition is suppressed. In addition, the dispersibility of the water-insoluble metal salt can be significantly improved.

<Addition of other components>
Also, at the same time as or before or after the addition of the above components, other components such as fragrances, essential oils, viscosity modifiers, foam modifiers, enzymes (for degradation of proteins, lipids, carbohydrates, etc.) are added. Also good.

<Content of each component>
In addition, since content of each component is as above-mentioned, detailed description is abbreviate | omitted here.

≪Principle of antibacterial action≫
Next, the principle of the antibacterial action of the antibacterial liquid composition on an object (food, tableware, food manufacturing apparatus, skin, etc.) will be described.

In the present invention, when the antibacterial component in the antibacterial liquid composition, that is, the above-mentioned water-insoluble metal salt is diluted with water or alcohol, a metal ion as a hydrophilic component and a surfactant as a lipophilic component ( Glycerin fatty acid ester, sorbitan fatty acid ester, sucrose fatty acid ester, etc.) are emulsified, that is, metal ions and surfactants form water-insoluble metal salts, which are polyglycerin fatty acid esters, etc. By being emulsified by the dispersant, an effect of inactivating bacteria and viruses is expressed. Specifically, it is understood that the present antibacterial liquid composition inactivates bacteria and viruses on the following principle.

First, a water-insoluble metal salt adheres to the surface membrane of a living body such as bacteria or virus. Next, the water-insoluble metal salt adhering to the surface membrane of the living body penetrates into the living body such as bacteria and viruses. Furthermore, the water-insoluble metal salt that has penetrated into the living body causes protein denaturation and coagulation in the living body of bacteria, viruses, etc., and denaturates virus particle antigens and bacterial glycoproteins in a low concentration and in a short time. Is inactivated. As described above, it is considered that the antibacterial liquid composition can inactivate bacteria, viruses, and the like attached to the object.

≪Use of antibacterial liquid composition≫
The application of the antibacterial liquid composition as described above is not particularly limited as long as it is an application that requires antibacterial / disinfecting / bacteriostatic or the like against bacteria, viruses, etc. This antibacterial liquid for uses such as antibacterial (bacterial and bacteriostatic) agents used in kitchen supplies, food production equipment, skin and other objects, bacteriostatic and bacteriostatic agents, food quality preservation agents, and cleaning agents Compositions can be used.

≪How to use antibacterial liquid composition≫
This antibacterial liquid composition can be undiluted or diluted and sprayed or applied to an object (food, tableware, kitchen utensils, food cleaning devices, skin, etc.), or the object can be applied to the composition. It can be used by a method such as dipping. Moreover, it does not specifically limit about methods, such as spraying, application | coating, and immersion, The method generally applied can be used.

Hereinafter, the present invention will be described more specifically with reference to examples. However, the following examples do not limit the present invention.

≪Dispersibility evaluation≫
First, the dispersibility of the water-insoluble metal salt was evaluated for Examples and Comparative Examples of the antibacterial liquid composition. Specifically, an evaluation test was performed as follows.

<Sample preparation method>
A sample of the antibacterial liquid composition used in this evaluation was prepared as follows. First, as described in Table 1 and Table 2 below, zinc sulfate or alum is dissolved in ion-exchanged water as a metal ion source having antibacterial properties, and the obtained aqueous solution is described in Table 1 and Table 2. Nonionic surfactant (glycerin fatty acid ester, sorbitan fatty acid ester, any one or two of sucrose fatty acid ester), polyglycerin fatty acid ester or polyoxyethylene sorbitan fatty acid ester as a dispersant, and alcohol are added, After stirring and mixing, ion-exchanged water was further added so that water was contained in the composition in the amounts shown in Tables 1 and 2. Finally, as a pH adjusting agent, the pH was adjusted to 5.5 to 6.0 using a combination of citric acid and arginine or sodium lactate, and Examples 1 to 21 (see Table 1) and Comparative Examples 1 to 17 ( Samples for evaluation of dispersibility as shown in Table 2 were obtained.

The surfactants and dispersants used in the examples and comparative examples are as follows.
Glycerin fatty acid ester: Glycerin monocaprylate Sorbitan fatty acid ester: Sorbitan octate Sucrose fatty acid ester: Sucrose monolaurate Polyglycerol fatty acid ester: Glycerin monolaurate Polyoxyethylene (hardened) castor oil: PEG40 Hydrogenated castor oil Polyoxy Ethylene polyoxypropylene glycol: Polyoxyethylene polyoxypropylene glycol (molecular weight 2000, EO content 20%)
Polyoxyethylene sorbitan fatty acid ester: polyoxyethylene (20) sorbitan oleate (polysorbate 80)

<Appearance evaluation method>
The dispersibility of the prepared sample was determined by visually observing the appearance of the sample immediately after preparation and after being left at 50 ° C. for 1 week, and evaluated according to the following criteria. did. The results of Examples 1 to 21 are shown in Table 1, and the results of Comparative Examples 1 to 17 are shown in Table 2.
○: Appearance is colorless and transparent even after standing for 1 week ○ △: Transforms to white transparent after standing for 1 week or a small amount of precipitate is generated Δ: White precipitate is generated after standing for 1 week ×: White precipitate immediately after preparation Occurs

<Evaluation results>
As shown in Table 1, the samples of Examples 1 to 21 all had good dispersibility. On the other hand, as shown in Table 2, Comparative Examples 1 to 8, 11 to 17 using polyoxyethylene sorbitan fatty acid ester as a dispersant for dispersing the water-insoluble metal salt, comparison containing no alcohol Examples 2, 4, 6, 8 to 10, 12, and 14 all showed inferior dispersibility (evaluation of Δ or ×). From the above results, as the antibacterial liquid composition according to the present invention, it contains a water-insoluble metal salt formed from a metal salt and a surfactant, and as a dispersant for dispersing the water-insoluble metal salt. Dispersibility of water-insoluble metal salt is good by using at least one of polyoxyethylene sorbitan fatty acid ester, polyoxyethylene (hardened) castor oil and polyoxyethylene polyoxypropylene glycol and containing alcohol. It was shown that a colorless and transparent liquid was obtained.

≪Evaluation of antibacterial and antiviral properties≫
Next, the antibacterial and antiviral properties of the antibacterial liquid composition were evaluated for the examples and comparative examples of the antibacterial liquid composition. Specifically, first, an evaluation test of the antibacterial liquid composition of Example 1 was performed as follows.

<Evaluation method>
The antibacterial property was evaluated by the following method for the following bacteria and viruses.

(S. aureus, Pseudomonas aeruginosa, Salmonella, enterohemorrhagic Escherichia coli, Campylobacter)
(A) For S. aureus, Pseudomonas aeruginosa, Salmonella, enterohemorrhagic Escherichia coli, Campylobacter, first, a sterilized test tube containing 10 mL of the antibacterial liquid composition of Example 1, and sterilized physiological saline as a control Five sets of test tubes containing 10 mL were prepared.
(B) About 1.5 × 10 ⁸ bacterial suspensions were prepared using strains cultured in brain heart infusion agar medium at 35 ° C. for 18 hours in 3 mL of sterilized physiological saline (Campylobacter A strain cultured on a Brucella HK agar medium at 37 ° C. for 24 hours was used).
(C) A test tube containing 100 μL of the bacterial suspension so that the number of bacteria in the test product is about 1.5 × 10 ⁶ cells / mL, and containing the test product of (a) and a control sterilized physiological saline. After 10 minutes and 20 minutes, 100 μL and 10 μL were sampled, inoculated into SCDLP agar plate medium, and observed by streak culture at 35 ° C. for 72 hours ( Campylobacter was observed on Brucella HK agar at 37 ° C. for 72 hours). The test article temperature during the test was 25 ° C., the room temperature was 25.5 ° C., and the humidity was 49%.

As a result of the tests as described above, those having an antibacterial effect were evaluated as ○, and those without were evaluated as ×.

(Shigella, Legionella, Clostridium perfringens, Bacillus cereus)
(A) For Shigella, Legionella, Clostridium perfringens, and Bacillus cereus, first, a sterilization test tube containing 10 mL of the antibacterial liquid composition of Example 1 and a test containing 10 mL of sterilized physiological saline as a control. Five sets of tubes were prepared.
(B) About 1.5 × 10 ⁸ bacterial suspensions were prepared using strains cultured in brain heart infusion agar medium at 35 ° C. for 18 hours in 3 mL of sterilized physiological saline. Legionella was cultured on a B-CYEα agar medium at 35 ° C. for 72 hours. 85% of the spores were obtained using cooked meat medium for the formation of spores.
(C) A test tube containing 100 μL of the bacterial suspension so that the number of bacteria in the test product is about 1.5 × 10 ⁶ cells / mL, and containing the test product of (a) and a control sterilized physiological saline. After 10 minutes, 100 μL each was sampled, inoculated into a SCDLP agar plate medium, and observed by streak culture at 35 ° C. for 72 hours (Legionella was CYE agar medium). Was observed for 120 hours at 35 ° C. Welsh bacteria were observed on Brucella HK agar medium at 35 ° C. for 72 hours). The test article temperature during the test was 25 ° C., the room temperature was 25 ° C., and the humidity was 49%.

(Influenza virus)
(A) Influenza virus (type A) is ingested into the chorioallantoic cavity of the developing chicken egg, cultured in an incubator, chorioallantoic fluid is collected, and a virus solution purified by density gradient centrifugation is used as a test virus solution .
(B) 900 μL of test solution and 100 μL of 2.3 × 10 ⁹ TCID ₅₀ / mL test virus solution were added to the test tube, gently mixed with a vortex mixer, and allowed to act at room temperature for a predetermined time. Thereafter, 100 μL was immediately collected and diluted 100-fold with 9.9 mL of cell maintenance medium (DMEM) containing 0.2% fetal bovine serum (FBS). This solution was used as a sample stock solution for a virus inactivation effect test to evaluate the virus inactivation effect. Note that phosphate buffered saline (PBS) was used in place of the test solution for the action time 0 minute and the negative control sample. As a positive control, ethanol for disinfection of Japanese Pharmacopoeia (Wako Pure Chemical Industries) was used instead of the test solution.

As a result of the tests as described above, those having an antiviral effect were evaluated as ○, and those without were evaluated as ×.

(Feline calicivirus (norovirus substitute))
(A) Feline calicivirus was infected with feline kidney-derived cells (CRFK), and when about 90% or more of the cell culture area showed cytopathic effect (CPE), it was stored frozen in a freezer at −80 ° C. Thereafter, the freeze-thaw operation was repeated twice, and the supernatant obtained by centrifugation at 3500 rpm for 10 minutes was collected, and a virus solution concentrated and purified with an ultrafiltration membrane was used as a test virus solution.
(B) 900 μL of the test solution and 100 μL of 4.2 × 10 ⁸ TCID ₅₀ / mL test virus solution were added to the test tube, gently mixed with a vortex mixer, and reacted at room temperature for a predetermined time. Immediately thereafter, 100 μL of the reaction solution was diluted 100-fold with 9.9 mL of phosphate buffered saline (PBS). This solution was used as a sample stock solution for a virus inactivation effect test to evaluate the virus inactivation effect. Note that phosphate buffered saline (PBS) was used in place of the test solution for the action time 0 minute and the negative control sample. As a positive control, ethanol for disinfection of Japanese Pharmacopoeia (Wako Pure Chemical Industries) was used instead of the test solution.

<Evaluation results>
The results of the above antibacterial and antiviral tests are shown in Table 3 below.

As shown in Table 3, the antibacterial liquid composition of Example 1 had an inactivating effect against any of the bacteria and viruses evaluated above. In Examples 2 to 21, the same antibacterial / antiviral test as in Example 1 was performed, and in each example, the same results as in Example 1 were obtained.

The preferred embodiment of the present invention has been described above, but the present invention is not limited to the above-described embodiment. That is, it is understood that other forms or various modifications that can be conceived by those skilled in the art within the scope of the invention described in the claims belong to the technical scope of the present invention.

Claims

A water-insoluble metal salt produced by a reaction between an antibacterial metal ion and a surfactant;
At least one dispersant selected from the group consisting of polyglycerin fatty acid ester, polyoxyethylene (cured) castor oil and polyoxyethylene polyoxypropylene glycol;
Alcohol and
Containing
The antibacterial liquid composition whose content of the said alcohol is 3 to 80 mass% on the basis of the mass of all the compositions.
The antibacterial liquid according to claim 1, wherein the metal ions having antibacterial properties are one or more ions selected from the group consisting of aluminum ions, zinc ions, copper ions, iron ions and silver ions. Composition.
The antibacterial liquid composition according to claim 2, wherein the metal ions having antibacterial properties are aluminum ions.
The surfactant according to any one of claims 1 to 3, wherein the surfactant is one or more nonionic surfactants selected from the group consisting of glycerin fatty acid ester, sorbitan fatty acid ester and sucrose fatty acid ester. The antibacterial liquid composition described in 1.
The antibacterial liquid composition according to any one of claims 1 to 4, wherein the dispersant is a polyglycerol fatty acid ester.
The antibacterial liquid composition according to any one of claims 1 to 5, wherein the amount of the surfactant with respect to the metal ion is 0.4 to 15 in terms of mass ratio.
The antibacterial liquid composition according to any one of claims 1 to 6, wherein the content of the dispersant is 0.001% by mass or more and 0.3% by mass or less based on the mass of the entire composition. .
The antibacterial liquid composition according to any one of claims 1 to 7, wherein the alcohol is a lower alcohol having 3 or less carbon atoms.