WO2014012584A1

WO2014012584A1 - Analysis device for examining a fluid in a flexible sac and reaction container having a wall made of a flexible material

Info

Publication number: WO2014012584A1
Application number: PCT/EP2012/064089
Authority: WO
Inventors: Matthias Dürr
Original assignee: Siemens Aktiengesellschaft
Priority date: 2012-07-18
Filing date: 2012-07-18
Publication date: 2014-01-23

Abstract

The invention relates to an analysis device (16) for examining a fluid (12, 12') in a container (10) having a flexible wall (14). The analysis device (1) comprises two clamping bodies (20, 22), which are designed to moved relative to each other between an open position and a closed position, wherein each clamping body (20, 22) has a clamping edge (26, 28). In the open position the two clamping edges (26, 28) are spaced apart from each other and define an insertion slot through which a part (30) of the container can be introduced into a holding region (30') delimited by the two clamping bodies (20, 22). In the closed position the two clamping edges (26, 28) are pressed against each other. As a result, the part (30) of the container (10) introduced into the holding region (30') is pinched off at least in sections from the remainder (36) of the container (10), so that when fluid (12) is present in the container (10) the pinched off part (30) forms a bulge (30) of the container (10) in the holding region (30'), the shape (38) of which bulge in the closed position is determined by the clamping bodies (20, 22).

Description

description

Analysis device for examining a fluid in a flexible bag and reaction container with a wall of a flexible material

The invention relates to an analysis device for examining a fluid, which is located in a container with a flexible wall. A fluid is understood here to mean a liquid, a gas or else a dispersion. The invention also includes a reaction vessel having a wall of flexible material defining a main volume for the introduction of a fluid. Finally, the invention also includes a method of inspecting a fluid in a flexible container. Specifically, the flexible container is a disposable reaction container as used in the pharmaceutical and food processing industry as a replacement for a reusable reaction container having a sterilizable surface.

Disposable reaction vessels are available, for example, from the company "GE Healthcare Lifescience" under the name of disposable bioreactors, which are plastic bags that can be made of polyethylene, for example. Disposable reaction vessels have the advantage of having their inner surfaces in front is solved first use, for example, for breeding cell cultures, not consuming ste ^¬ must be rilisiert. While thus the problem of cleaning the container, the question arises, like the other, required for the process components prepared favorably may be used. in particular, measurement ^¬ appliances and sensors for monitoring of a chemical process in a disposable reaction vessel must be as pure when they are immersed in the reservoir fluid present in the reaction. It is known for this purpose to provide disposable sensors which drive in the fluid and transmit measured values, for example via an RFID radio connection (RFID - Radio-Frequency Identification), to a receiving station with a measuring device which is located outside the reaction container.

The object of the present invention is to reduce the risk of conta ^¬ mination of the fluid by sensors or measuring instruments in the analysis of a fluid in a container and to eliminate the need for cleaning.

The object is achieved by an analysis device according to patent ^¬ claim 1, reaction container according to claim 9 and claim 13 and a method according to claim 14. Advantageous developments of the invention are given by the dependent claims.

The analysis apparatus according to the invention is suitable for examining a fluid which is located in a container having a flexible wall, so ^¬ hose such as a plastic bag or a cut plastic, as used in laboratories for the manufacture of disposable containers with a predeterminable volume normally. The analyzer can be clamped to such a flexible plastic bag, whereby one on the bag a

Clamp the bead in which the fluid can be examined. In this case, one uses the special properties of liquid-filled or gas-filled sacks or bags, which bulge out due to the internal pressure of the fluid contained therein and thus develop a weak dimensional stability, but still allow a deformation of the flexible outer wall without any problems. Thus, it is possible to pinch off a fold or bead, which is connected at both ends with the rest of the fluid body, although in the middle of the shape of a closed tube or a bead has, similar to a "bacon" Unclamping a part of the container resulting geomet ^¬ cally defined form opens procedural possibilities However, these are not allowed by the geometrically undefined bag body itself. Thus, to pump within the clamped region, the fluid or len abzutei- a volume geometrically defined and observed as the Mög ^¬ friendliness. The particular advantage of this is that this is possible without contact with the contents of the bag. Since ^¬ with a handling of the fluid in the flexible container is possible without this, the tools used with the fluid itself come into connection.

The analytical device according to the invention now has, for forming the described bead on a flexible bag, two clamping bodies which are designed to be movable relative to one another between an open position and a closed position. For example, it may be a tube which is divided along its tube axis, in which case each tube half represents one of the clamping body. Each clamping body has a clamping edge. For a split pipe, these may be the cut edges. In the open position, the two clamping edges are spaced apart, so that an insertion gap is formed, through which a part of the flexible container, namely that part which is to form the bead, can be inserted between the two clamping bodies in a receiving area, ie approximately in the Inside of the pipe. If you fold then the two clamping members together, these take the closed position a, in which the two clamping edges up are pressed together and thereby is at least partially clamped in the Aufnahmebe ^¬ rich located between the clamping part of the body of the container from the rest of the container. By sections it is meant that the clamped part may still be open at its ends to the main volume of the container. A fluid, eg located in the disconnected part of the container is a liquid, bil ^¬ det the disconnected part of the desired bead on the Behäl- ter. Since the bead is clamped between the clamping bodies, the shape of the bead is also predetermined by the shape of the clamping body in the closed position of the analysis device, since the flexible wall of the containers is attached to the clamping bodies. lies. This gives the desired geometric boundary conditions in the region of the bead. The two clamping bodies can be U-shaped in order to make it easier to clamp them to a generally curved surface of a bag.

A preferred embodiment of the analysis device provides that on opposite sides of the receiving area, in which the bead is located, in each case a transparent area or a passage opening is provided in the clamping bodies. Can then be through a through opening, for example a light beam or an ultrasonic signal in the fluid initiate and received by the other through hole ^¬ again. Thus, optical and sound-based investigations of the fluid are possible without having to open the container for this purpose.

Preferably, the analyzer has a pumping means, which then provides the advantage that within the channel formed by the bead, the fluid can be moved or conveyed, thereby pumping the fluid into the main volume of the container and at the same time pumping further fluid from the main volume into the bead can be. This allows a biological or chemical reaction to be observed in the bulk volume by pumping reaction products into the channel regularly for analysis. The pumping device comprises at least one pressure body and a control device, which is designed to press the bead located in the receiving area in response to a pump signal at one end with the pressure body. This reduces the volume in the bead and thus displaces the fluid, without the pressure body or another part of the pumping device coming into contact with the fluid itself. The pressing on the bead pressure body act like a

Peristaltic pump, by means of which from outside the fluid in the

Bead can be moved. When using pressure bodies results in a further advantage that also a divisible pump can be formed, which together with the two Clamping bodies can be folded around a part of the container.

A variant of the pumping device provides that a balloon or another expandable body is provided as the pressure body. The control device has in this case a hydraulic or pneumatic system for inflating the pressure hull. The at least one pressure body can also be designed round and then be rolled over the bead by a drive element of the control device, so that it is rolled. This also results in a displacement effect within the bead.

It is particularly preferred that the pumping means comprises a plurality of pressure bodies, which are arranged in at least one row. The pressure bodies then rest along the longitudinal direction of extension of the bead on one side thereof or on opposite sides on the bead. By now pressing the pressure bodies successively on the bead, a pumping movement in the bead is brought about with the pressure bodies according to the principle of peristalsis. This is similar to the swallowing function in the esophagus of a man whose muscles along the esophagus can transport a fluid in the esophagus in a similar way to the pressure vessels.

A further advantage results if a second pumping device is provided at the other end of the bead, so that this end can also be pressed in by means of the at least one pressure ^{element of} the second pumping device. As a result, a pressure in the fluid can be regulated in the bead, as it may be necessary for a measurement.

The clip-analysis device allows by Wan ^¬ extension of the container through the fluid on the basis of electromagnetic radiation, that is visible in particular Light, UV radiation or infrared radiation, and / or on the basis of sound to investigate contamination. An embodiment of the analysis device provides in this connection a coupling device for the mechanical coupling of an examination device. In particular, a Ankop ^¬ peleinrichtung for an examination apparatus is provided which allows at least one of the following tests: a Ro ^¬ tationsdispersion, a colorimetry, a photometry, a granulometry.

The analysis device of the invention described can be attached to any disposable reaction container having a flexible wall, are so formed as to a Plas ^¬ tikbeutel or a plastic tube, the walls of which in each case of a flexible film of plastic.

However, the invention also provides a special variant of a reaction vessel with a wall made of a flexible Ma ^¬ TERIAL. The wall provides a prepared area for the attachment of the clamp body. The reaction vessel according to the invention has, in principle, the shape of a bag, ie the wall of the flexible material defines a main ^¬ volume for filling of the fluid, wherein the material is just impermeable to the fluid. In the inventive reaction container is a branching off from the main volume of tubular channel is now formed which is open at its respective ends to the main volume, ie, the channel communicates via its open ends with the Hauptvo ^¬ lumen. The reaction vessel according to the invention has the advantages in part, that the geometric dimensions of the channel can be very precisely defined, in particular his diam ^¬ ser. Furthermore, the reaction container for clamping the channel need not be subjected to a force, so that the risk is reduced that the material breaks when attaching the analyzer.

The preformed channel, which corresponds to the bead, as by means of the analyzer on any bulky Pouch can be formed in the inventive ^¬ sen reaction vessel may be partially formed on the wall bounding the main volume. Thus the channel may play form a handle protruding to the reaction vessel at ^¬. As a result, the fluid in the channel can then be examined without the reaction container having to be pressed in.

The channel can also be formed at least partially by embossing and / or welding of those film, through which the wall of the reaction vessel is formed. This makes it a particularly economical variation of erfindungsge ^¬ MAESSEN reaction vessel provide. Improved optical properties arise when at least a portion of the channel is formed from a tube of a more ^rigid material, in particular of plastic or glass. Then one is not dependent on the optical properties of the flexible material from which the remainder of the reaction vessel is formed.

In order to enable different type measurements still further, provides an embodiment provides that the channel has a measurement area, in which at least one of the following measuring elements is arranged in a wall of the channel: one for at least one component of the fluid permeable Memb ^¬ ran, so a semipermeable membrane, and / or electrical contacts. Such special functional surfaces make it possible, for example, to measure the oxygen content, the pH, the temperature, the pressure, the conductivity and / or the humidity in the fluid without polluting or contaminating the measuring instrument itself Measuring instrument enters the fluid. The membrane is preferably disposed of with the disposable bag.

The invention also includes a reaction vessel with a flexible wall, in which the described measuring ranges are provided independently of a preformed channel. The necessary channel can be formed here simply by means of the erfindungsge ^¬ MAESSEN analysis device by clamping a bead. In the following, the invention will be explained in more detail again with reference to concrete exemplary embodiments. This shows:

1 shows a schematic diagram of a frontal view of a disposable reaction vessel made of a flexible material to which a preferred execution ^¬ form of the analysis apparatus according to the invention is mounted,

2 shows a schematic representation of a side view of the disposable reaction container of FIG. 1,

3 shows a schematic representation of a cross section through the analysis device on the reaction container of FIG. 1, FIG.

4 shows a schematic representation of a more detailed

5 is a schematic representation of a top view of the analysis apparatus on the reaction vessel of FIG. 1, FIG.

6 shows a schematic diagram of a granulometry measurement, as can be carried out with the analysis device on the reaction vessel of FIG. 1,

7 is a schematic representation of a longitudinal section through two peristaltic pumps, which may be part of the analysis device according to the invention, FIG 8 is a schematic representation of a longitudinal section through a hose pump which can be provided in the erfindungsge ^¬ MAESSEN analysis device, FIG 9 is a schematic diagram of a frontal view of one embodiment of a reaction vessel according to the invention with the measuring surfaces which may have a membrane or electrical contacts, Figure 10 is a schematic Representation of a cross section through the measuring surfaces of the reaction container of FIG 9,

11 shows a further embodiment of the invention

Reaction container with a molded channel and

FIG 12 is a schematic representation of an embodiment of the reaction vessel according to the invention with inserted ^¬ prägtem channel.

A first embodiment of the analysis device according to the invention is explained below with reference to FIG 1 to FIG 5 tert ^¬ tert. In the figures, a reaction container 10 is shown, which may for example be a bag or bag made of a film of a plastic, for example polyethylene. In the reaction vessel may be a fluid 12, for example a liquid, a dispersion or a gas. In the present case it is assumed that it is a bag and is sigkeit wherein Reaktionsbe ^¬ container 10 in the fluid 12 is a liquid-. For example, it may be too fer ^¬ mentierende liquid. To the bag 10 14 ei ^¬ ne analysis device 16 is clamped from the outside ^¬ SEN to a film formed by the flexible wall. The analysis device 16 comprises a hinged housing 18, which may have the overall shape of a U-shaped bent tube. The housing 18 may be formed from two housing halves 20, 22, which are mounted by means of a hinge (not shown) in a folding area 24 against each other movable. be- see two edges 26, 28 of the two housing halves 20, 22 is a part of the flexible wall 14 of the bag 10 is clamped. The two edges 26, 28 may be pressed together, for example, by springs arranged in the hinged region 24 (not shown). As a result, a bead 30 is separated from the bag 10. To form the bead 30, were the two Ge ^¬ housing halves 20, pressed against the wall 14 of the bag 10 22 and folded such that emerged between the housing ^¬ halves 20, 22 when folding the bead 30 in the form of egg nes pipe or channel is.

In the bead 30 is also a part of the fluid, which is provided here with the reference numeral 12 '. The flexible material 14 of the bag 10 in the interior of the housing 18 is pressed by the fluid 12 'against the housing halves 20, 22, so that the bead 30 takes the form of an analysis volume 30' delimited by the housing halves 20, 22. The wall 14 of the bag 10 around the bead 30 thus forms a channel whose cross-sectional profile and in particular whose diameter 38 is determined by the shape of the housing halves 20, 22. The fluid 12 contained in the bead 30 'can jeweili ^¬ ge, open ends 32, 34 of the bead flow to the main volume 36 of the bag 10 30th A center piece 40 of the housing 18 may include two windows located at gegenüberlie ^¬ constricting sides of the bead 30 or openings 42, 44 have for studies of the fluid 12 located in the bead 30 '. Basically, conditions for a continuous measurement by means of optical and optionally also acoustic ^¬ tischer method (eg ultrasound) are given to a geometrically defined sample volume in the analysis volume 30 '. The sample volume is in this case given by the fluid 12 'in the bead 30. Due to the known diameter 38, the methods for the measurement can be parameterized. FIG. 6 shows by way of example how granulometry can be carried out in the bead 30. By means of a gepuls ^¬ th light source 46 such as a laser, is generated a light beam in the example shown, which is widened in a beam expander 48 to an expanded light beam 50 through the passage opening 42 and the film which the wall 14 of the bead 30 forms, in the Flu ^¬ id 12 'passes, passes through this and emerges from the Öff ^¬ tion 44 again from the middle piece 40. The emitted light beam 50 is then bundled again by means of a lens 52 and directed onto a camera 54, which is coupled to a (not shown) processor unit for the ^per se known granulometric evaluation. By means of the granulometry arrangement shown, for example, cells can be measured and other particle size determinations can be carried out.

In a comparable manner, an optical Rotationsdis ^¬ persion can be carried out, by means of which can be determined the concentra- tion of optically active substances by means of an irradiation of the fluid 12 ', polarization and angle measurement. For example, the sugar content in a solution can be determined. Another embodiment provides for the performance of a colorimetry, in which colors of a sample 30 located in the bead of the fluid 12 'with a

Reference or a spectral analysis breaks down the color of the sample. This is also possible in the infrared and ultraviolet range. It is then important to ensure that the flexible material from which the wall 14 of the bag 10 is made, for the radiation used is correspondingly permeable or at least the influence of the material is taken into account in the evaluation. By means of colorimetry, the water hardness may be determined, for example who are ^¬ examines the or a biological sample such as urine or blood.

A further embodiment provides for carrying out a photometry, by means of which concentration measurements of Solutions on a weakening of the luminous intensity of an electromagnetic radiation, such as a light beam, by absorption in the fluid 12 'is performed. All the listed measurements can be carried out with a known diameter 38 of the sample. At the same time non-disposable capable of measuring devices can be used because the Mes ^¬ solution is completely free of contamination, that is there is no contact with the fluid 12 '. Thus, in total by the examples shown game that can be extended to expensive high-performance measurement technology with the analysis device 16 of the disposable ^¬ advantage of bags, without thereby a significant modification to one of the two technologies would be required. In the analysis device 16 can also be one or two

Pump devices 56, 58 may be provided, which may be located, for example, on both sides of the center piece 40 in the housing 18 in front of the ends 32, 34 (see FIG 7). The two pumping devices can be designed to be divisible, so that they can be moved with the housing halves 20, 22 and can be folded around the wall 14 of the bag 10 when attaching the analysis device 16. By a geeigne ^¬ te control of the two pumping devices 56, 58, the chamber formed by the bead 30 can be shut off or continuously flowed through with fresh fluid 12 from a main volume 36 of the bag 10.

One possible embodiment of the pump follows the pattern of peristaltic movement of the esophagus. For this purpose, for example hydraulically or pneumatically in their diameter variable pressure body 60 are pumped in a predetermined sequence and relaxed again to pump the fluid 12 'in the bead 30 in ^¬ example in the middle part 40 and out of this addition ^¬ pump. In the position of the pressure bodies shown in FIG. 7, both pump devices 56, 58 are blocked. This is a snapshot of a pumping movement. The flow direction 62 points in FIG. 7 from left to right through the bead 30. In the drainage-pumping device 58 the bead 30 is opened straight to the passage opening 34 toward the main volume 36.

Another form of divisible pump is shown in FIG. 8 shows a hose pump 64, which presses with rotating Ku ^¬ rules, discs or rollers or other rotary, round moldings 66, the bead 30 and thereby between the mold bodies 66 supports each of the fluid portions 12 '. The shaped bodies 66 likewise represent pressure bodies.

Another aspect of the invention is the use of special bags, which simplify the area for the attachment of the analysis device 16 with pump and the ^{¬ out} in the middle part 40 ^¬ formed chamber for diagnosis and provide additional diagnostic options. In FIGS. 9 and 10, a reaction container is shown for this purpose, which, for example, can be a bag 68 made of a flexible film 70. In the film 70 three coupling elements 72 are attached in the example shown. In FIG. 10, one of the coupling elements 72 is shown in cross-section. A cylindrical body 74 of the coupling element 72 has a collar 76, on which a measuring device ^¬ example as can be fixed by means of a snap connection. A flange 78 is connected to the film 70, ^beispielswei se welded. For example, the flange 78 and the foil 70 may be ultrasonically welded or laser welded. The film 70 has a hole in the region of the coupling element 72. In this through hole or recess 80 of the film 70 projects a ring 82 of the coupling element 72 in the bag 68 into it. Due to the welded connection between flange 78 and foil 70, the recess 80 is sealed. In the ring 82, a membrane 84 is held. The membrane 84 is a functional ^surface via which a measuring device can interact with a fluid 86 located in the bag 68. For example, it may be in the membrane 84 to a semi-permeable membrane which may for example be formed from a stretched over ^¬ Teflon film. The volume defined by the ring 74 may also be mixed with a saline solution or another required for a measurement or liquid egg ^¬ nem gas to be filled.

The membrane 84 may also include an electrical conductive layer or a via. Thus, other measurements can be made possible such as a measurement of the Sauer ^¬ solid content, the pH, the temperature, pressure (when the diaphragm 84, for example, having strain gauges), of the conductivity and the humidity.

In Figures 11 and 12 is shown how by an adapted form of a reaction vessel, the reliability of the Anbrin ^¬ supply of the analysis apparatus 16, the height of the producible in the bead 30 pressure by means of the pumps, the accuracy of the geometric dimensions and optical quality of the

Passage areas in the area of the bead can be increased.

In FIG 11, a bag 88 is shown, in which a hose piece 90 is integrally formed on a film 92 which limits a Hauptvo ^¬ lumen 94 of the bag 88. The tube piece 90 is connected at both ends 96, 98 with the film 92, so that a diagnostic path of a channel 100 results, which opens at both ends 96, 98 in the main volume 94. A center piece 102 of the tube piece 90 can be made of a design optimized for an optical or acoustic measurement material best ^¬ hen, for example, from a piece of tube made of glass (eg, crystal glass) is made. The bag 88 can be filled with fluid via an opening 104. By the require- mung of the tube piece 90 from the outside there is a through ^¬ handle portion 106, so that an analysis device can fully enclose the central piece 102nd

In FIG 12, a bag 108 is shown, which can be filled via an opening 110. In a region 114 and 116, the two opposite walls of the bag 108 are connected to each other. For this purpose, the film 112 of the wall can be welded, pressed together and / or hot embossed be. Between the two regions 114, 116, a channel 118 is formed, which communicates via its two channel ends 120, 122 with a main volume 124 of the bag 108. The Ka ^¬ nal 118 is in the bag 108 particularly easy len herzustel-. Moreover, he does not stand above the basic shape of the bag

108 also so that the channel 118 can entangle and nowhere hän ^¬ genbleiben. By the section 114 welded already been or is stamped, an analyzer must apply kei ^¬ ne particularly large force to disconnect the channel 118 from the main volume 24th

Reference sign list

10 reaction vessels

12, 12 'fluid

14 wall

16 analysis device

18 housing

20, 22 housing half

24 joint area

26, 28 edge

30 bead

30 'analysis volume

32 passage opening

36 main volume

40 middle piece

42, 44 passage opening

46 light source

48 beam expansion

50 light beam

52 lens

54 camera

56, 58, 64 pumping device

60 pressure hull

62 flow direction

66 role

68 bags

70 foil

72 coupling element

74 basic body

76 collar

78 flange

80 passage opening

82 ring

84 membrane or electrical contacts

86 fluid

88 bags

90 hose piece

92 foil Main olumen

98 end

channel

Centerpiece of the canal

opening

Through region

bag

opening

foil

, 116 Welded area

channel

, 122 canal end

Main olumen

Claims

claims

An analysis device (16) for inspecting a fluid (12, 12 ') _/ which is located in a container (10) with a flexible wall (14), comprising a first and a two ^¬ th clamping body (20, 22) each clamping body (20, 22) has a clamping edge (26, 28) and in the open position, the two clamping edges (26, 28) are spaced from each other and limit a Ein ^¬ guide gap through which a portion (30) of the Behäl ^¬ ters in one of the two clamping bodies (20, 22) defined receiving area (30 ') is insertable, and in the closed position the two clamping edges (26, 28) consecu- are dergedrückt and thereby the in the receiving area

(30 ') introduced part (30) of the container (10) is at least from ^¬ cut off from the rest (36) of the container (10) is clamped, so that the clamped part (30) located in the container (10) fluid (12) in the receiving region (30 ') forms a bead (30) of the container (10) whose shape (38) in the closed position by the clamping body (20, 22) is predetermined.

2. Analysis device (16) according to claim 1, characterized in that in at least one of the clamping body (20, 22) on opposite sides of the receiving region (30 ') each have a transparent region or a through hole (42, 44) is provided.

3. Analysis device (16) according to any one of claims 1 or 2, characterized by a pumping device (56, 58, 64) with at least one pressure body (60, 66) and a control device which is adapted to, in the receiving area (30 '). located bead (30) on a pump signal towards an end of the bead (30) with the pressure body (60, 66) depress.

4. Analysis device (16) according to claim 3, characterized in that the at least one pressure body (60) stretchable and said control means comprises a hydraulic or pneuma ^¬ schematic system for inflating the pressure body (60).

5. An analysis device (16) according to claim 3 or 4, character- ized in that the at least one pressure body (66) is round and the control device comprises a drive element which is adapted to roll the bead (30) with the pressure body ,

6. Analysis device (16) according to any one of claims 3 to 5, characterized in that the pumping means (56, 58) a plurality of pressure bodies (60) which are arranged in at least one row, and the control device is designed to ^¬ , the Press the end of the bead (30) in succession with the pressure bodies (60) in accordance with the principle of peristaltics.

7. Analysis device (16) according to any one of claims 3 to 6, characterized by a second pumping device (56, 58) of the type mentioned, which is adapted to press a further end of the bead (30) by means of its at least one pressure body (60) ,

8. Analysis device (16) according to one of the preceding claims, characterized by a coupling device (40) for the mechanical coupling of an examination device (48, 52) for an examination of the fluid (12 ') on the basis of electromagnetic radiation, in particular light, and / or sound, in particular for at least one of the following sub ^¬ investigations: a rotatory dispersion, a colorimetry, a photometry, a granulometry.

A reaction vessel (88, 108) in which a wall (92, 112) of flexible material defines a main volume (94, 124) for filling a fluid, the material being impermeable to the fluid,

characterized in that

a tubular channel (100, 118) branching off from the main volume (94, 124) is formed, which at its respective end (96, 98, 120, 122) communicates with the main volume (94, 124).

10. reaction container (88) according to claim 9, characterized in that the channel (100) is at least partially formed on the main volume (94) bounding wall (92).

11. reaction container (108) according to claim 9 or 10, characterized in that the channel (118) is at least partially formed by embossing and / or welding a film (112) through which the wall of the container (108) is formed.

12. reaction container (88) according to one of claims 9 to 11, characterized in that at least a portion of the Ka ^¬ nals of a tube (102) made of a different material of the wall material, in particular a rigid material, preferably plastic or Glass, is formed.

13. reaction container (68), characterized in that a flexible wall (70) of the reaction container (68) has a measuring ^¬ range, in which at least one of the following measuring elements (72) is disposed in the wall: one for at least one ^¬ Component of the fluid permeable membrane (84), electrical contacts (84).

14. A method of inspecting a fluid (12) in a flexible container (10, 68, 88, 108),

characterized by the steps:

Forming a tubular channel (30, 100, 118) having a known diameter (38) from a portion of the container (10, 68, 88, 108),

Separating a part of the fluid (12 ') into the channel (30, 100, 118),

- Examine the located in the channel (30, 100, 118)

Fluids (12 ') at least with an investigation method, which is based on electromagnetic radiation and / or sound.

15. The method according to claim 14, characterized by the step:

- conveying the fluid (12 ') within the channel (30) and thereby exchanging the fluid (12') in the channel (30) with further fluid (12) located in a main volume (36) of the container (10).