WO2014012584A1 - Dispositif d'analyse permettant d'analyser un fluide dans une poche souple; et récipient de réaction comportant une paroi réalisée en un matériau souple - Google Patents

Dispositif d'analyse permettant d'analyser un fluide dans une poche souple; et récipient de réaction comportant une paroi réalisée en un matériau souple Download PDF

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Publication number
WO2014012584A1
WO2014012584A1 PCT/EP2012/064089 EP2012064089W WO2014012584A1 WO 2014012584 A1 WO2014012584 A1 WO 2014012584A1 EP 2012064089 W EP2012064089 W EP 2012064089W WO 2014012584 A1 WO2014012584 A1 WO 2014012584A1
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WO
WIPO (PCT)
Prior art keywords
fluid
container
bead
analysis device
channel
Prior art date
Application number
PCT/EP2012/064089
Other languages
German (de)
English (en)
Inventor
Matthias Dürr
Original Assignee
Siemens Aktiengesellschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Siemens Aktiengesellschaft filed Critical Siemens Aktiengesellschaft
Priority to PCT/EP2012/064089 priority Critical patent/WO2014012584A1/fr
Publication of WO2014012584A1 publication Critical patent/WO2014012584A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/505—Containers for the purpose of retaining a material to be analysed, e.g. test tubes flexible containers not provided for above
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/26—Constructional details, e.g. recesses, hinges flexible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/28—Constructional details, e.g. recesses, hinges disposable or single use
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61J—CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
    • A61J1/00—Containers specially adapted for medical or pharmaceutical purposes
    • A61J1/05—Containers specially adapted for medical or pharmaceutical purposes for collecting, storing or administering blood, plasma or medical fluids ; Infusion or perfusion containers
    • A61J1/10—Bag-type containers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/088—Channel loops
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/036—Cuvette constructions transformable, modifiable
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0364—Cuvette constructions flexible, compressible
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/21—Polarisation-affecting properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251—Colorimeters; Construction thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/29—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using visual detection
    • G01N21/293—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using visual detection with colour charts, graduated scales or turrets
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G01N21/80—Indicating pH value

Definitions

  • the invention relates to an analysis device for examining a fluid, which is located in a container with a flexible wall.
  • a fluid is understood here to mean a liquid, a gas or else a dispersion.
  • the invention also includes a reaction vessel having a wall of flexible material defining a main volume for the introduction of a fluid.
  • the invention also includes a method of inspecting a fluid in a flexible container.
  • the flexible container is a disposable reaction container as used in the pharmaceutical and food processing industry as a replacement for a reusable reaction container having a sterilizable surface.
  • Disposable reaction vessels are available, for example, from the company "GE Healthcare Lifescience” under the name of disposable bioreactors, which are plastic bags that can be made of polyethylene, for example. Disposable reaction vessels have the advantage of having their inner surfaces in front is solved first use, for example, for breeding cell cultures, not consuming ste ⁇ must be rilinstrument. While thus the problem of cleaning the container, the question arises, like the other, required for the process components prepared favorably may be used.
  • measurement ⁇ appliances and sensors for monitoring of a chemical process in a disposable reaction vessel must be as pure when they are immersed in the reservoir fluid present in the reaction. It is known for this purpose to provide disposable sensors which drive in the fluid and transmit measured values, for example via an RFID radio connection (RFID - Radio-Frequency Identification), to a receiving station with a measuring device which is located outside the reaction container.
  • RFID radio connection RFID - Radio-Frequency Identification
  • the object of the present invention is to reduce the risk of conta ⁇ mination of the fluid by sensors or measuring instruments in the analysis of a fluid in a container and to eliminate the need for cleaning.
  • the analysis apparatus is suitable for examining a fluid which is located in a container having a flexible wall, so ⁇ hose such as a plastic bag or a cut plastic, as used in laboratories for the manufacture of disposable containers with a predeterminable volume normally.
  • the analyzer can be clamped to such a flexible plastic bag, whereby one on the bag a
  • the fluid or lenucktei- a volume geometrically defined and observed as the Mög ⁇ friendliness.
  • the particular advantage of this is that this is possible without contact with the contents of the bag. Since ⁇ with a handling of the fluid in the flexible container is possible without this, the tools used with the fluid itself come into connection.
  • the analytical device now has, for forming the described bead on a flexible bag, two clamping bodies which are designed to be movable relative to one another between an open position and a closed position.
  • it may be a tube which is divided along its tube axis, in which case each tube half represents one of the clamping body.
  • Each clamping body has a clamping edge.
  • these may be the cut edges.
  • the two clamping edges are spaced apart, so that an insertion gap is formed, through which a part of the flexible container, namely that part which is to form the bead, can be inserted between the two clamping bodies in a receiving area, ie approximately in the Inside of the pipe.
  • the shape of the bead is also predetermined by the shape of the clamping body in the closed position of the analysis device, since the flexible wall of the containers is attached to the clamping bodies. lies. This gives the desired geometric boundary conditions in the region of the bead.
  • the two clamping bodies can be U-shaped in order to make it easier to clamp them to a generally curved surface of a bag.
  • a preferred embodiment of the analysis device provides that on opposite sides of the receiving area, in which the bead is located, in each case a transparent area or a passage opening is provided in the clamping bodies. Can then be through a through opening, for example a light beam or an ultrasonic signal in the fluid initiate and received by the other through hole ⁇ again.
  • a through opening for example a light beam or an ultrasonic signal in the fluid initiate and received by the other through hole ⁇ again.
  • the analyzer has a pumping means, which then provides the advantage that within the channel formed by the bead, the fluid can be moved or conveyed, thereby pumping the fluid into the main volume of the container and at the same time pumping further fluid from the main volume into the bead can be.
  • the pumping device comprises at least one pressure body and a control device, which is designed to press the bead located in the receiving area in response to a pump signal at one end with the pressure body. This reduces the volume in the bead and thus displaces the fluid, without the pressure body or another part of the pumping device coming into contact with the fluid itself.
  • the pressing on the bead pressure body act like a
  • Bead can be moved.
  • pressure bodies results in a further advantage that also a divisible pump can be formed, which together with the two Clamping bodies can be folded around a part of the container.
  • a variant of the pumping device provides that a balloon or another expandable body is provided as the pressure body.
  • the control device has in this case a hydraulic or pneumatic system for inflating the pressure hull.
  • the at least one pressure body can also be designed round and then be rolled over the bead by a drive element of the control device, so that it is rolled. This also results in a displacement effect within the bead.
  • the pumping means comprises a plurality of pressure bodies, which are arranged in at least one row.
  • the pressure bodies then rest along the longitudinal direction of extension of the bead on one side thereof or on opposite sides on the bead.
  • a pumping movement in the bead is brought about with the pressure bodies according to the principle of peristalsis. This is similar to the swallowing function in the esophagus of a man whose muscles along the esophagus can transport a fluid in the esophagus in a similar way to the pressure vessels.
  • a further advantage results if a second pumping device is provided at the other end of the bead, so that this end can also be pressed in by means of the at least one pressure element of the second pumping device. As a result, a pressure in the fluid can be regulated in the bead, as it may be necessary for a measurement.
  • the clip-analysis device allows by Wan ⁇ extension of the container through the fluid on the basis of electromagnetic radiation, that is visible in particular Light, UV radiation or infrared radiation, and / or on the basis of sound to investigate contamination.
  • An embodiment of the analysis device provides in this connection a coupling device for the mechanical coupling of an examination device.
  • a Ankop ⁇ pel coupled for an examination apparatus is provided which allows at least one of the following tests: a Ro ⁇ tationsdispersion, a colorimetry, a photometry, a granulometry.
  • the analysis device of the invention described can be attached to any disposable reaction container having a flexible wall, are so formed as to a Plas ⁇ tik milk or a plastic tube, the walls of which in each case of a flexible film of plastic.
  • the invention also provides a special variant of a reaction vessel with a wall made of a flexible Ma ⁇ TERIAL.
  • the wall provides a prepared area for the attachment of the clamp body.
  • the reaction vessel according to the invention has, in principle, the shape of a bag, ie the wall of the flexible material defines a main ⁇ volume for filling of the fluid, wherein the material is just impermeable to the fluid.
  • the inventive reaction container is a branching off from the main volume of tubular channel is now formed which is open at its respective ends to the main volume, ie, the channel communicates via its open ends with the Hauptvo ⁇ lumen.
  • the reaction vessel according to the invention has the advantages in part, that the geometric dimensions of the channel can be very precisely defined, in particular his diam ⁇ ser.
  • the reaction container for clamping the channel need not be subjected to a force, so that the risk is reduced that the material breaks when attaching the analyzer.
  • the preformed channel which corresponds to the bead, as by means of the analyzer on any bulky Pouch can be formed in the inventive ⁇ sen reaction vessel may be partially formed on the wall bounding the main volume.
  • the channel may play form a handle protruding to the reaction vessel at ⁇ .
  • the fluid in the channel can then be examined without the reaction container having to be pressed in.
  • the channel can also be formed at least partially by embossing and / or welding of those film, through which the wall of the reaction vessel is formed. This makes it a particularly economical variation of erfindungsge ⁇ MAESSEN reaction vessel provide. Improved optical properties arise when at least a portion of the channel is formed from a tube of a more rigid material, in particular of plastic or glass. Then one is not dependent on the optical properties of the flexible material from which the remainder of the reaction vessel is formed.
  • the channel has a measurement area, in which at least one of the following measuring elements is arranged in a wall of the channel: one for at least one component of the fluid permeable Memb ⁇ ran, so a semipermeable membrane, and / or electrical contacts.
  • the membrane is preferably disposed of with the disposable bag.
  • the invention also includes a reaction vessel with a flexible wall, in which the described measuring ranges are provided independently of a preformed channel.
  • the necessary channel can be formed here simply by means of the erfindungsge ⁇ MAESSEN analysis device by clamping a bead.
  • FIG. 1 shows a schematic diagram of a frontal view of a disposable reaction vessel made of a flexible material to which a preferred execution ⁇ form of the analysis apparatus according to the invention is mounted,
  • FIG. 2 shows a schematic representation of a side view of the disposable reaction container of FIG. 1,
  • FIG. 3 shows a schematic representation of a cross section through the analysis device on the reaction container of FIG. 1, FIG.
  • FIG. 5 is a schematic representation of a top view of the analysis apparatus on the reaction vessel of FIG. 1, FIG.
  • FIG. 6 shows a schematic diagram of a granulometry measurement, as can be carried out with the analysis device on the reaction vessel of FIG. 1,
  • FIG 7 is a schematic representation of a longitudinal section through two peristaltic pumps, which may be part of the analysis device according to the invention
  • FIG 8 is a schematic representation of a longitudinal section through a hose pump which can be provided in the erfindungsge ⁇ MAESSEN analysis device
  • FIG 9 is a schematic diagram of a frontal view of one embodiment of a reaction vessel according to the invention with the measuring surfaces which may have a membrane or electrical contacts
  • Figure 10 is a schematic Representation of a cross section through the measuring surfaces of the reaction container of FIG 9,
  • FIG 12 is a schematic representation of an embodiment of the reaction vessel according to the invention with inserted ⁇ yogtem channel.
  • a reaction container 10 which may for example be a bag or bag made of a film of a plastic, for example polyethylene.
  • the reaction vessel may be a fluid 12, for example a liquid, a dispersion or a gas.
  • a fluid 12 for example a liquid, a dispersion or a gas.
  • it is assumed that it is a bag and is stechnik wherein Christsbe ⁇ container 10 in the fluid 12 is a liquid-.
  • To the bag 10 14 ei ⁇ ne analysis device 16 is clamped from the outside ⁇ SEN to a film formed by the flexible wall.
  • the analysis device 16 comprises a hinged housing 18, which may have the overall shape of a U-shaped bent tube.
  • the housing 18 may be formed from two housing halves 20, 22, which are mounted by means of a hinge (not shown) in a folding area 24 against each other movable.
  • be- see two edges 26, 28 of the two housing halves 20, 22 is a part of the flexible wall 14 of the bag 10 is clamped.
  • the two edges 26, 28 may be pressed together, for example, by springs arranged in the hinged region 24 (not shown).
  • a bead 30 is separated from the bag 10.
  • the bead 30 is also a part of the fluid, which is provided here with the reference numeral 12 '.
  • the flexible material 14 of the bag 10 in the interior of the housing 18 is pressed by the fluid 12 'against the housing halves 20, 22, so that the bead 30 takes the form of an analysis volume 30' delimited by the housing halves 20, 22.
  • the wall 14 of the bag 10 around the bead 30 thus forms a channel whose cross-sectional profile and in particular whose diameter 38 is determined by the shape of the housing halves 20, 22.
  • a center piece 40 of the housing 18 may include two windows located at governmentlie ⁇ constricting sides of the bead 30 or openings 42, 44 have for studies of the fluid 12 located in the bead 30 '.
  • conditions for a continuous measurement by means of optical and optionally also acoustic ⁇ tischer method are given to a geometrically defined sample volume in the analysis volume 30 '.
  • the sample volume is in this case given by the fluid 12 'in the bead 30. Due to the known diameter 38, the methods for the measurement can be parameterized.
  • FIG. 6 shows by way of example how granulometry can be carried out in the bead 30.
  • a gepuls ⁇ th light source 46 such as a laser
  • a beam expander 48 to an expanded light beam 50 through the passage opening 42 and the film which the wall 14 of the bead 30 forms, in the Flu ⁇ id 12 'passes, passes through this and emerges from the ⁇ ff ⁇ tion 44 again from the middle piece 40.
  • the emitted light beam 50 is then bundled again by means of a lens 52 and directed onto a camera 54, which is coupled to a (not shown) processor unit for the per se known granulometric evaluation.
  • the granulometry arrangement shown for example, cells can be measured and other particle size determinations can be carried out.
  • an optical Rotationsdis ⁇ persion can be carried out, by means of which can be determined the concentra- tion of optically active substances by means of an irradiation of the fluid 12 ', polarization and angle measurement.
  • the sugar content in a solution can be determined.
  • Another embodiment provides for the performance of a colorimetry, in which colors of a sample 30 located in the bead of the fluid 12 'with a
  • Reference or a spectral analysis breaks down the color of the sample. This is also possible in the infrared and ultraviolet range. It is then important to ensure that the flexible material from which the wall 14 of the bag 10 is made, for the radiation used is correspondingly permeable or at least the influence of the material is taken into account in the evaluation.
  • the water hardness may be determined, for example who are ⁇ examines the or a biological sample such as urine or blood.
  • a further embodiment provides for carrying out a photometry, by means of which concentration measurements of Solutions on a weakening of the luminous intensity of an electromagnetic radiation, such as a light beam, by absorption in the fluid 12 'is performed. All the listed measurements can be carried out with a known diameter 38 of the sample. At the same time non-disposable capable of measuring devices can be used because the Mes ⁇ solution is completely free of contamination, that is there is no contact with the fluid 12 '. Thus, in total by the examples shown game that can be extended to expensive high-performance measurement technology with the analysis device 16 of the disposable ⁇ advantage of bags, without thereby a significant modification to one of the two technologies would be required. In the analysis device 16 can also be one or two
  • Pump devices 56, 58 may be provided, which may be located, for example, on both sides of the center piece 40 in the housing 18 in front of the ends 32, 34 (see FIG 7).
  • the two pumping devices can be designed to be divisible, so that they can be moved with the housing halves 20, 22 and can be folded around the wall 14 of the bag 10 when attaching the analysis device 16.
  • the chamber formed by the bead 30 can be shut off or continuously flowed through with fresh fluid 12 from a main volume 36 of the bag 10.
  • One possible embodiment of the pump follows the pattern of peristaltic movement of the esophagus.
  • hydraulically or pneumatically in their diameter variable pressure body 60 are pumped in a predetermined sequence and relaxed again to pump the fluid 12 'in the bead 30 in ⁇ example in the middle part 40 and out of this addition ⁇ pump.
  • both pump devices 56, 58 are blocked. This is a snapshot of a pumping movement.
  • the flow direction 62 points in FIG. 7 from left to right through the bead 30.
  • the bead 30 is opened straight to the passage opening 34 toward the main volume 36.
  • FIG. 8 shows a hose pump 64, which presses with rotating Ku ⁇ rules, discs or rollers or other rotary, round moldings 66, the bead 30 and thereby between the mold bodies 66 supports each of the fluid portions 12 '.
  • the shaped bodies 66 likewise represent pressure bodies.
  • FIGS. 9 and 10 Another aspect of the invention is the use of special bags, which simplify the area for the attachment of the analysis device 16 with pump and the ⁇ out in the middle part 40 ⁇ formed chamber for diagnosis and provide additional diagnostic options.
  • a reaction container is shown for this purpose, which, for example, can be a bag 68 made of a flexible film 70.
  • three coupling elements 72 are attached in the example shown.
  • one of the coupling elements 72 is shown in cross-section.
  • a cylindrical body 74 of the coupling element 72 has a collar 76, on which a measuring device ⁇ example as can be fixed by means of a snap connection.
  • a flange 78 is connected to the film 70, beispielswei se welded.
  • the flange 78 and the foil 70 may be ultrasonically welded or laser welded.
  • the film 70 has a hole in the region of the coupling element 72. In this through hole or recess 80 of the film 70 projects a ring 82 of the coupling element 72 in the bag 68 into it. Due to the welded connection between flange 78 and foil 70, the recess 80 is sealed.
  • a membrane 84 is held in the ring 82.
  • the membrane 84 is a functional surface via which a measuring device can interact with a fluid 86 located in the bag 68. For example, it may be in the membrane 84 to a semi-permeable membrane which may for example be formed from a stretched over ⁇ Teflon film.
  • the volume defined by the ring 74 may also be mixed with a saline solution or another required for a measurement or liquid egg ⁇ nem gas to be filled.
  • the membrane 84 may also include an electrical conductive layer or a via.
  • other measurements can be made possible such as a measurement of the Sauer ⁇ solid content, the pH, the temperature, pressure (when the diaphragm 84, for example, having strain gauges), of the conductivity and the humidity.
  • Figures 11 and 12 is shown how by an adapted form of a reaction vessel, the reliability of the Anbrin ⁇ supply of the analysis apparatus 16, the height of the producible in the bead 30 pressure by means of the pumps, the accuracy of the geometric dimensions and optical quality of the
  • Passage areas in the area of the bead can be increased.
  • a bag 88 in which a hose piece 90 is integrally formed on a film 92 which limits a Hauptvo ⁇ lumen 94 of the bag 88.
  • the tube piece 90 is connected at both ends 96, 98 with the film 92, so that a diagnostic path of a channel 100 results, which opens at both ends 96, 98 in the main volume 94.
  • a center piece 102 of the tube piece 90 can be made of a design optimized for an optical or acoustic measurement material best ⁇ hen, for example, from a piece of tube made of glass (eg, crystal glass) is made.
  • the bag 88 can be filled with fluid via an opening 104.
  • a bag 108 is shown, which can be filled via an opening 110.
  • the two opposite walls of the bag 108 are connected to each other.
  • the film 112 of the wall can be welded, pressed together and / or hot embossed be.
  • a channel 118 is formed, which communicates via its two channel ends 120, 122 with a main volume 124 of the bag 108.
  • the Ka ⁇ nal 118 is in the bag 108 particularly easy len herzustel-. Moreover, he does not stand above the basic shape of the bag

Abstract

L'invention concerne un dispositif d'analyse (16) permettant d'analyser un fluide (12, 12') dans un récipient (10) ayant une paroi (14) souple. Le dispositif d'analyse (16) comprend deux corps de serrage (20, 22) conçus de manière à ce que qu'ils soient mobiles entre une position ouverte et une position fermée, chacun des corps de serrage (20, 22) comportant un bord de serrage (26, 28). Dans la position ouverte, les deux bords de serrage (26, 28) sont espacés l'un de l'autre et délimitent une fente d'introduction à travers laquelle une partie (30) du récipient peut être introduite dans une zone de réception (30') délimitée par les deux corps de serrage (20, 22). Dans la position fermée, les deux bords de serrage (26, 28) appuient l'un sur l'autre. Ainsi, la partie (30) du récipient (10) qui a été introduite dans la zone de réception (30') est au moins partiellement séparée du reste (36) du récipient (10) par serrage, faisant en sorte que la partie (30) séparée par serrage constitue, lorsque le récipient (10) contient du fluide (12), un bourrelet (30) lequel est situé dans la zone de réception (30') du récipient (10) et lequel adopte, dans la position fermée, une forme (38) prédéfinie par les corps de serrage (20, 22).
PCT/EP2012/064089 2012-07-18 2012-07-18 Dispositif d'analyse permettant d'analyser un fluide dans une poche souple; et récipient de réaction comportant une paroi réalisée en un matériau souple WO2014012584A1 (fr)

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PCT/EP2012/064089 WO2014012584A1 (fr) 2012-07-18 2012-07-18 Dispositif d'analyse permettant d'analyser un fluide dans une poche souple; et récipient de réaction comportant une paroi réalisée en un matériau souple

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017203249A1 (fr) * 2016-05-24 2017-11-30 The Technology Partnership Plc Système destiné à être utilisé dans le biotraitement
WO2022133105A1 (fr) * 2020-12-18 2022-06-23 Lonza Biologics Inc. Déconnexion stérile de systèmes de bioprocédé et son procédé d'utilisation

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JPS57144463A (en) * 1981-03-02 1982-09-07 Olympus Optical Co Ltd Automatic analysis and reaction vessel used for this method
US4522494A (en) * 1982-07-07 1985-06-11 The United States Of America As Represented By The Department Of Health And Human Services Non-invasive optical assessment of platelet viability
WO1992019764A1 (fr) * 1991-05-08 1992-11-12 Baxter Diagnostics Inc. Procede et appareil permettant de detecter la contamination bacterienne du sang destine aux transfusions
US20080171383A1 (en) * 2006-11-03 2008-07-17 Finesse Solutions, Llc. Optical interface for disposable bioreactors
US20080286155A1 (en) * 2007-05-15 2008-11-20 Polestar Technologies, Inc. Multilayered optical sensing patch and retaining plug therefor
DE202012004503U1 (de) * 2011-05-10 2012-05-22 Sartorius Stedim Biotech Gmbh Einweg-Sensorkopf und Einwegbehälter

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57144463A (en) * 1981-03-02 1982-09-07 Olympus Optical Co Ltd Automatic analysis and reaction vessel used for this method
US4522494A (en) * 1982-07-07 1985-06-11 The United States Of America As Represented By The Department Of Health And Human Services Non-invasive optical assessment of platelet viability
WO1992019764A1 (fr) * 1991-05-08 1992-11-12 Baxter Diagnostics Inc. Procede et appareil permettant de detecter la contamination bacterienne du sang destine aux transfusions
US20080171383A1 (en) * 2006-11-03 2008-07-17 Finesse Solutions, Llc. Optical interface for disposable bioreactors
US20080286155A1 (en) * 2007-05-15 2008-11-20 Polestar Technologies, Inc. Multilayered optical sensing patch and retaining plug therefor
DE202012004503U1 (de) * 2011-05-10 2012-05-22 Sartorius Stedim Biotech Gmbh Einweg-Sensorkopf und Einwegbehälter

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017203249A1 (fr) * 2016-05-24 2017-11-30 The Technology Partnership Plc Système destiné à être utilisé dans le biotraitement
EP3889248A1 (fr) * 2016-05-24 2021-10-06 TTP plc Système destiné à être utilisé dans le biotraitement
US11236298B2 (en) 2016-05-24 2022-02-01 Ttp Plc System for use in bioprocessing
WO2022133105A1 (fr) * 2020-12-18 2022-06-23 Lonza Biologics Inc. Déconnexion stérile de systèmes de bioprocédé et son procédé d'utilisation

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