WO2013174387A1 - Système d'obtention d'un spectre optique - Google Patents

Système d'obtention d'un spectre optique Download PDF

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Publication number
WO2013174387A1
WO2013174387A1 PCT/DK2013/050157 DK2013050157W WO2013174387A1 WO 2013174387 A1 WO2013174387 A1 WO 2013174387A1 DK 2013050157 W DK2013050157 W DK 2013050157W WO 2013174387 A1 WO2013174387 A1 WO 2013174387A1
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WIPO (PCT)
Prior art keywords
porous filter
region
sers
active material
pores
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PCT/DK2013/050157
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English (en)
Inventor
Sokol Ndoni
Fengxiao GUO
Lars Schulte
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Danmarks Tekniske Universitet
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Publication of WO2013174387A1 publication Critical patent/WO2013174387A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons

Definitions

  • the optical analysis system is arranged for obtaining the optical spectrum of the analytes adjacent to the SERS-active material exclusively from the second region of the porous filter, such as arranged for obtaining the optical spectrum exclusively from the second region so as to exclude contributions to the optical spectrum from relatively large analytes which are too large to travel through the porous filter from the first region to the second region.
  • the invention is particularly, but not exclusively, advantageous for providing to the skilled person (i.e., enabling obtaining, such as fabricating or preparing) a system for obtaining an optical spectrum of analytes in a fluid sample, wherein the porous filter may serve to filter the sample and the SERS-active material may serve to enhance a signal so that the quality of the optical spectrum is improved.
  • the porous filter may serve to filter the sample and the SERS-active material may serve to enhance a signal so that the quality of the optical spectrum is improved.
  • the SERS active surface is integrated in the porous filter, since this enables a compact system, and furthermore provides the possibility that the porous filter may house the SERS- active material and/or may shape the SERS-active material so as to enhance the SERS effect.
  • a light source is understood to be a device capable of emitting light. Examples of light sources may include Light Emitting Diodes (LED's) or laser sources.
  • LED's Light Emitting Diodes
  • laser sources may include Light Emitting Diodes (LED's) or laser sources.
  • Filtration is understood as the mechanical or physical operation which is used for the separation of entities above a certain size from fluids (liquids or gases) by interposing a filter, such as a multilayer lattice, such as a porous filter, through which only the fluid, and possible also smaller entities within the fluid, can pass. Large solids in the fluid are retained since they cannot pass through the filter.
  • a filter such as a multilayer lattice, such as a porous filter
  • a sensor element wherein an effective average dia meter of pores of the porous filter is inferior to, such as significantly inferior to, a wavelength, la mbda, of light transm itted through the sensor element, such as light incident upon the SERS active material or em itted from the SERS active material .
  • the effective refractive index is given as a weighted average between the two refractive indices of material of the porous filter and the med ium located within the pores of the porous filter.
  • a more accurate estimation of the effective refractive index is achieved by using e.g . the Lorentz-Lorenz m ixing rule : Where n ef r, ⁇ and n 2 are the effective refractive index, the refractive index of the filter polymer and the refractive index of the medium filling the pores,
  • the porous filter material may acts as a filter by blocking entities, such as fat pa rticles or other relatively la rge entities, which are not small enoug h to pass through the pores.
  • the second region comprises the SERS active material, so that the light sig na l em itted from the second region, due to the SERS active materia l, effectively dominates the light detected so that the optical spectrum is dom inated by the light em itted from the second reg ion .
  • the time it takes for a fluid sample, such as pure water, and/or the sufficiently small analytes (for example a solute molecule of radius 0.5 nm, representative for a number of antibiotics, which may use roughly 50% more time than water to penetrate through a pore of radius 5 nm) in the fluid sample to go from the first region to the second region is less than 1 minute, such as less than 30 seconds, such as less than 10 seconds, such as less than 5 seconds, such as less than 2 seconds, such as less than 1 second, such as less than 500 msec, such as less than 200 msec, such as less than 100 msec, such as less than 50 msec.
  • first region and the second region are spatially spaced
  • SERS Surface Enhanced Raman Scattering
  • a SERS-active surface is understood to be a surface which will enhance the Raman scattering of photons by analyte molecules positioned adjacent thereto.
  • the SERS-active surface may be the surface of a SERS-active material.
  • the SERS-active surface comprises topographical structures with dimensions on the order of hundreds of nanometers, such as tens of nanometers, such as nanometers.
  • the SERS-active surface comprises topographical structures with dimensions which are relatively small compared to the wavelength of the light being emitted onto the second region by the optical analysis system.
  • the SERS-active surface comprises topographical structures with dimensions which are comparable to the pore diameter of the pores in the porous filter, such as equal to or smaller than the pore diameter of the pores in the porous filter, such as smaller than the pore diameter of the pores in the porous filter.
  • the SERS active material being placed at least partially within the pores of the porous filter is understood that at least some portion of the SERS active material is placed at least partially within the pores of the porous filter.
  • coherent entities of SERS active material such as nanoparticles of SERS active material, is placed within the pores of the porous filter.
  • an optical analysis system is understood any system which may enable obtaining an optical spectrum.
  • An optical filter is understood to be an entity which allows passage of light within a certain region or regions of the optical spectrum whereas it blocks light within other wavelength regions.
  • the concept of an optical filter is also understood to comprise other means enabling selection of certain
  • a porous filter with pores having a pore diameter being below 900 nm, such as below 800 nm, such as below 700 nm, such as below 600 nm, such as below 500 nm, such as below 400 nm, such as below 300 nm, such as below 200 nm, such as below 100 nm, such as below 90 nm, such as below 80 nm, such as below 70 nm, such as below 60 nm, such as below 50 nm, such as below 40 nm, such as below 30 nm, such as below 20 nm, such as below 15 nm, such as below 10 nm, such as below 5 nm, such as below 2 nm, such as below 1 nm.
  • a possible advantage of having pores below a certain size may be that they enable filtering of analytes above a certain size.
  • a possible advantage of having pores below a certain size may be that they enable filtering of analytes above a certain size.
  • a porous filter with pores having a pore diameter being above 50 nm, such as above 40 nm, such as above 30 nm, such as above 20 nm, such as above 15 nm, such as above 10 nm, such as above 5 nm, such as above 2 nm, such as above 1 nm.
  • a possible advantage of having pores above a certain size may be that filtering time is kept relatively low.
  • a possible advantage of having pores below a certain size may be that the SERS enhancement is larger for SERS active material with features above this size, and that consequently the pores may serve as shaping elements in order to provide SERS active material with appropriately sized features.
  • a porous filter with pores having a pore diameter being within 5-100 nm, such as within 5-50 nm, such as within 5-20 nm.
  • the pore diameter is within 5 nm-1000 nm, such as within 5 nm-500 nm, such as within 5 nm-300 nm.
  • the pore diameter is within 10 nm-1000 nm, such as within 10 nm-500 nm, such as within 10 nm- 300 nm.
  • the pore diameter is within 20 nm-1000 nm, such as within 20 nm-500 nm, such as within 20 nm-300 nm.
  • An advantage of having the pore diameter being within certain bounds may be that the SERS effect is particularly pronounced within these bounds, which for example may enable the pores to endow a SERS active material placed thereon a relatively large SERS enhancing effect by transferring their physical dimensions to the SERS enhancing material.
  • the SERS effect weakens both for too large (in an example, more than ⁇ 300 nm) and too small (in an example, less than 20 nm) particles. Roughly speaking for too large particles, so-called multipole electromagnetic radiation develops, and as SERS enhancement happens only through the dipole radiation, then the more multipoles are active and more energy is radiated in SERS non active modes, therefore less SERS enhancement. So particles or roughness features being too large become less efficient and the effect is too little beyond, for example, 0.5 - 1 micron. For too small particles (for example less than ⁇ 5 nm) there is not enough conductivity and the plasmons, which are collective
  • the optical spectrum is a fluorescence spectrum.
  • fluorescence spectrum is understood an optical spectrum of an analyte which enables determining the energy levels of the electronic states of the analyte.
  • An advantage of obtaining the Fluorescence spectrum of an analyte might be that it provides insight into the chemical bonds and symmetry of molecules of the analyte. Therefore, it may provide a fingerprint by which the molecule can be identified.
  • the SERS- active material comprises a plurality of nanoparticles being placed within the pores of the porous filter.
  • the system further comprises a processor arranged for
  • a system wherein the porous filter is patterned into at least two different area types, where the different area types differ with respect to each other in a degree of hydrophilicity, so as to enable controlling a movement of a liquid in the fluid sample placed on the first region of the porous filter.
  • a possible advantage of this may be that it enables guiding the fluid sample, such as a liquid in the fluid sample, placed on the first region through, e.g., hydrophilic channels in the porous filter. It is noted that this may in particular be effective when the fluid sample comprises a liquid, such as an aqueous liquid.
  • hydrophilicity' is used as a quantitative property, indicating how hydrophilic or hydrophobic a surface is. Specifically, when referring to
  • a droplet of fluid on the surface can have any contact angle, including a contact angle corresponding to a hydrophilic or hydrophobic surface, i.e., a surface associated with a degree of hydrophilicity can be hydrophilic or hydrophobic.
  • providing a porous filter patterned into at least two different area types may be realized by carrying out the method as described in the patent application WO 2010/066782 Al which is hereby incorporated by reference in entirety.
  • providing a porous filter patterned into a least two different area types may be realized by carrying out by the method as described in the patent application WO 2011/0050044 Al which is hereby incorporated by reference in entirety.
  • a system wherein the system comprises a plurality of second regions, and wherein the optical analysis system is arranged for obtaining optical spectra of the analytes adjacent to the SERS-active material in each of the second regions of the porous filter.
  • An advantage of this may be that it enables obtaining, such as measuring, optical spectra corresponding to multiple analytes thus increasing the capacity of the system.
  • Another advantage of this may be that it enables obtaining, such as measuring, optical spectra corresponding to the same type of analyte in multiple second regions, thus increasing the reliability of the system in terms of providing a reliable optical spectrum corresponding to the analyte.
  • a system wherein the pore diameter is different in a bulk portion and/or the first region of the porous filter is smaller than at the second region, such as the pore diameter in a bulk portion and/or the first region of the porous filter may be 5-50 nm, such as 10-30 nm, such as the pore diameter in the second region of the porous filter may be 50-500 nm, such as 100-500 nm. It may be understood that the ranges does not include the endpoints.
  • the invention further relates to database comprising at least one predetermined optical spectrum of an analyte, wherein the
  • predetermined optical spectrum is determined using a system according to the first aspect.
  • Such database may be advantageous for later comparing optical spectra from the system according to the first aspect.
  • chemical adsorption which is actually creating a chemical bond between the analyte and the SERS active material, new signals can appear in SERS that are absent in normal Raman.
  • the intensities in such spectra might be specific to the type of system according to the first aspect.
  • the database may be a computer readable storage medium, such as a hard disk drive, the storage medium may in particular embodiments be any one of volatile, non-volatile, random access, digital, magnetic, data storage devices.
  • porous filter with pores having a pore diameter being below 1 micrometer, such as being below 500 nm, such as being below 200 nm, such as being below 100 nm, such as being below 50 nm, the porous filter being arranged so that the fluid sample may be placed onto a first region of the porous filter,
  • optical analysis system is arranged for optically probing analytes adjacent to the SERS-active material exclusively from the second region of the porous filter.
  • nanoparticles inside the pores such as forming the nanorparticles in situ inside the pores, such as by precipitation, such as by chemical reduction, such as by electrochemical reduction,
  • the nanoparticles may be formed, such as formed within the pores, by chemical reduction, or by electrochemical reduction, such as described in the article "Electrochemical Synthesis of Silver Nanoparticles", Rodriquez-Sanchez et al., J. Phys. Chem. B 2000, 104, 9683-9688, which is hereby incorporated by reference in entirety.
  • the invention relates to use of the optical system according to the first aspect for obtaining an optical spectrum of a filtered sample.
  • the sample may for example be any one of, e.g., milk, beverage, drinking water, waste water, blood, urine, sweat, saliva or other body fluids.
  • the first, second and third and fourth aspect of the present invention may each be combined with any of the other aspects.
  • FIG 1 shows deposition of a material 106 onto the porous filter material 102.
  • the deposition process may be embodied by any deposition process commonly known in the art, including electron-beam (e-beam) deposition, sputter deposition, electrochemical deposition in fluid, vapour deposition, chemical vapour deposition (CVD), physical vapour deposition (PVD), cathode sputtering, pyrolysis, and ion plating. It is understood that the deposited material may form a coherent layer, such as a layer of material in one piece, or may form separated islands of material on the porous filter material 102.
  • e-beam electron-beam
  • sputter deposition electrochemical deposition in fluid
  • vapour deposition chemical vapour deposition (CVD), physical vapour deposition (PVD)
  • PVD physical vapour deposition
  • cathode sputtering cathode sputtering
  • pyrolysis pyrolysis
  • ion plating
  • the material 106 may be placed within the pores of the porous filter material 102 by electrotemplating in a manner similar to the procedure described in the article "Controlled Photooxidation of Nanoporous Polymers", Ndoni et al., Macromolecules 2009, 42, 3877-3880, which article is hereby incorporated by reference in its entirety together with the corresponding Supporting Information.
  • the article shows realization of electrotemplating of copper within the pores of a nanoporous, polymeric material.
  • the preparation of the porous filter material is similar to the preparation of the polymer filter membranes described in the first paragraph (entitled “Membrane preparation") of the “METHODS” section of the article “Gyroid Nanoporous Membranes with Tunable Permeability", Li et al., ACS Nano 2011 5 (10), 7754-7766, which article is hereby included by reference in entirety. It is noted that the material preparation is described in the first three paragraphs of the 'Experimental' section of the article "Nanoporous materials from stable and metastable structures of 1,2-PB-b-PDMS block copolymers” , Schulte et al., Polymer 52 (2011), 422-429, which article is hereby included by reference in entirety.
  • the preparation of the porous filter material is similar to the preparation of the polymer filter membranes described in WO
  • a porous filter material may act as a filter, such as a filter for filtering a fluid.
  • nanoporous filter materials are applicable as filters, such as filters for
  • Rhodamine B molecules of approximately 1 nm may be in the filtrate where their fluorescent properties can be detected, and that the filter material can block entry of larger particles (such as 22 nm beads).
  • Two other techniques that may be employed to create nanoporous polymeric films are phase separation and ion-track etching.
  • FIG 4 shows an illustration of how an embodiment of the invention may function.
  • a porous filter material 102a whereupon a SERS active material 410a has been deposited.
  • the thickness 422 of the porous filter material 102a may in exemplary embodiments be within 1-1000 micrometer, such as within 10-100 micrometer.
  • the thickness 424 of the deposited SERS active material 410a may in exemplary embodiments be within 1-100 nanometer, such as within 5-50 nanometer, such as 10 nanometer.
  • the circle 412 denotes a region which comprises a portion of the first region, which is shown in an enlarged version in the upper part of FIG 4.
  • the circle 414 denotes a region which comprises a portion of a second region, which is shown in an enlarged version in the bottom part of FIG 4.
  • the pore diameter 421 of the pores is less than 1 micrometer, such as being below 500 nm, such as being below 200 nm, such as being below 100 nm, such as being below 50 nm.
  • the pores may enable the porous filter material to act as a filter by blocking entry of large entities, such as the entity 420, which is too large to enter into the pore. Smaller entities, such as the smaller entity 418 may enter into the pores.
  • the porous filter material may acts as a filter by blocking entities, such as fat particles or other relatively large entities, which are not small enough to pass through the pores.
  • FIG 4 In the bottom part of FIG 4 is shown a portion 102c of the porous filter material and a portion 410c of the SERS active material.
  • the deposited SERS active material 410a has entered into the pores 104c so that it forms small structures 416 which have a limited size due to the limited size of the pores.
  • the smaller structures 416 may enable enhancement of a Raman signal, since roughness on the scale of the pore diameter may be beneficial for enhancing the Raman signal.
  • small entities 418 such as a small molecule, may be probed using Raman, and due to the enhancement may be detected with higher accuracy, precision and/or lower detection limit.
  • FIG 6 shows a system for obtaining an optical spectrum 648, such as Raman spectrum or a fluorescence spectrum, of analytes in a fluid sample, such as a liquid sample or a gaseous sample, the system comprising
  • SERS-active material 610a, 610b having a SERS-active surface being placed at least partially within the pores of the porous filter within a second region of the porous filter, wherein the first region and the second region of the porous filter are spatially separated and connected by through-going pores so that only sufficiently small analytes are able to reach the second region, and
  • optical analysis system is arranged for obtaining the optical spectrum 648 of the analytes adjacent to the SERS-active material 610a, 610b exclusively from the second region of the porous filter 602a, 602b.
  • the light source 634 emits broad band light 636 and the wavelength selecting means is embodied by an optical filter 638 which selects a specific wavelength so that narrow band light is 640 is used for probing the analytes in the first region of the porous filter material.
  • the wavelength selecting means may also be realised by other means known in the art, for example a tunable light source, such as a tunable laser, or an optical filter being in the path of the light between the first region of the porous filter material 602a, 602b and the detector 642.
  • the detector 642 may be part of a commercial Raman spectrometer 644 which may be relatively compact cf., the scale bar 646 which corresponds to 10 centimetres.
  • One possible advantage of the setup shown in FIG 6 is that the LASER light is confined within two outer metal SERS substrates 610a, 610b, and multiple scattering of the LASER light may further reinforce the SERS response.
  • the first region may be the upper surface or upper region, such as the surface and region encircled by circle 712, and the second region may be the lower surface of the region at the interface between porous filter material 102a and deposited SERS active material 410a.
  • the distance between the first region and the second region may thus correspond to the thickness 422 of the porous filter material 102a.
  • FIG 8 shows an embodiment similar to the embodiment of FIG 7, except that instead of having a layer of SERS active material deposited on the porous filter material 802, nanoparticles 810 are embedded into the pores of the porous filter material within a second region with thickness 824.
  • Analytes being placed adjacent to the SERS active material 810 may give rise to surface enhanced Raman scattering which may be seen as emitted electromagnetic radiation 841a, 841b, which may in turn be detected and give rise to an optical spectrum which may be indicative of the presence (and potentially quantity) of the analytes.
  • FIG 9 shows an embodiment similar to the embodiment in FIG 8 except that the nanoparticles 910 are distributed throughout the porous filter material 902, and that the incoming light 940 is arranged for only probing a relatively short distance 924 into the porous filter material.
  • the lower portion of the porous filter material 902 being below the dotted line may be seen as a second region, and the upper portion, such as the upper surface, or the region adjacent the upper surface, may be seen as the first region.
  • the first region is separated by the lower region by distance 922.
  • FIG 9 also shows a pipette 926 placing a fluid sample 928 on the first region of a porous filter material 902.
  • the probing light as well as the emitted light may be arranged to propagate only across a relatively short distance within the porous material, which may be advantageous since a large path length within the porous material may generate a background signal from Raman scattering from the porous material and solute or gas which will overlap with the SERS signal from the second region.
  • FIG 10 shows Raman spectra of 1 mM biphenyl-4-thiol in ethanol solution deposited on top of a nanoporous polybutadiene of gyroid morphology.
  • the lower spectrum 1050 at the bottom is for the sample without the SERS enhancing substrate, while the upper two spectra show SERS signals of similar enhancement produced by either gold sputtering (middle spectrum 1052) or silver e-beam evaporation (upper spectrum 1054). The spectra were shifted vertically for clarity.
  • Electron beam evaporation of silver or gold was performed on Alcatel SCM600 with deposition rates of 1-10 A/s at a pressure of 2- 10 "6 mbar.
  • the sample showing the upper (grey line) SERS signal in the figure was covered with 40 nm of silver on one side at a deposition rate of 1 A/s.
  • FIGS 11-13 demonstrates the filtering properties of a nanoporous polymer, and more particularly shows filtration of milk through hydrophilic nanoporous cross- linked 1,2-PB.
  • FIG 11 shows two pieces of nanoporous polymer, one hydrophobic 1156 and the other hydrophilic 1158 which are both immersed into milk 1162. After 1 min a rising clear meniscus 1160 is observed in the case of the hydrophilic polymer piece. This is part of milk's whey entering the pores and rising by capillary forces.
  • FIG 13 shows that after drying the hydrophobic piece 1156 remains completely transparent, while the hydrophilic piece 1158 becomes hazy in the region previously filled by the clear liquid.
  • the haziness is ascribed to crystallites formed inside the nanopores mainly from whey sugars, which constitute the largest part of whey's dry mass.
  • FIG 14 shows an illustration of an embodiment of the invention similar to FIG 4, except that in the present figure, the pores 1404 (of the porous filter of porous filter material 1402) have a non-constant pore-size, such as pore diameter. More particularly, in the present embodiment, the pore diameter is different in a bulk portion and/or the first region of the porous filter than at the second region, such as at a surface of the porous filter where SERS active material 1410 may be deposited.
  • An advantage of this may be, that it enables tailoring the pore size(s) to suit different requirements, such as
  • the pore diameter in the bulk of the porous filter 1402 being optimized with respect to a filtering effect (such as enabling filtering a certain size of analytes) and/or optimized with respect to filtering time (such as the time it takes for fluid and analytes to flow in direction 1464 from the first region to the second region), and/or
  • the pore diameter in the second region being optimized so as to enable shaping the SERS active material 1410 in order to optimize the SERS enhancing effect.
  • an advantage of such embodiment may be that optimal filtering and/or optimal SERS enhancement may be achieved.
  • the pore diameter may be larger in the second region compared to a pore diameter in a bulk portion of the porous filter.
  • the pore diameter in a bulk portion and/or the first region of the porous filter may be 5-50 nm, such as 10-30 nm.
  • the pore diameter in the second region of the porous filter may be 50-1000 nm, such as 100-500 nm.
  • the a porous filter with non-constant pore sizes may be provided according to, for example, the reference “Selective Separation of Similarly Sized Proteins with Tunable Nanoporous Block Copolymer Membranes", Xiaoyan Qiu et al., ACS Nano, 2013, 7 (1), pp 768-776, which is hereby incorporated by reference in entirety.
  • a system for obtaining an optical spectrum 648 of analytes in a fluid sample wherein a porous filter 602a, 602b is arranged so that the fluid sample may be placed onto a first region of the porous filter, and a SERS-active material 610a, 610b having a SERS-active surface is placed at least partially within the pores of the porous filter within a second region of the porous filter.
  • the first region and the second region of the porous filter are spatially separated and connected by through-going pores so that only sufficiently small analytes are able to reach the second region.
  • the porous filter enables that the fluid sample is filtered so that only sufficiently small entities in the fluid sample reach the second region where they may be probed so that an optical spectrum related to the analytes in the filtered sample may be obtained.
  • the optical system also comprises a light source 634, a light detector 642, and the optical analysis system is arranged for obtaining the optical spectrum 648 of the analytes adjacent to the SERS-active material 610a, 610b in the second region, such as exclusively from the second region, of the porous filter 602a, 602b.
  • the invention may relate to:
  • a porous filter (602a, 602b) with pores having a pore diameter being below 1 micron, the porous filter being arranged so that the fluid sample may be placed onto a first region of the porous filter, a SERS-active material (610a, 610b) having a SERS-active surface being placed at least partially within the pores of the porous filter within a second region of the porous filter, wherein the first region and the second region of the porous filter are spatially separated and connected by through-going pores so that only sufficiently small analytes are able to reach the second region, and
  • optical analysis system comprising
  • optical analysis system is arranged for obtaining the optical spectrum (648) of the analytes adjacent to the SERS-active material (610a, 610b) in the second region of the porous filter (602a, 602b).
  • SERS-active material (602a, 602b) is part of a layer of coherent material with dimensions being larger than the pore diameter.
  • SERS-active material comprises a plurality of nanoparticles (206, 308) being placed within the pores of the porous filter (102).
  • system further comprises a processor arranged for
  • porous filter (602a, 602b) is patterned into at least two different area types, where the different area types differ with respect to each other in a degree of hydrophilicity, so as to enable controlling a movement of a liquid in the fluid sample placed on the first region of the porous filter.
  • the optical analysis system comprises a plurality of second regions, and wherein the optical analysis system is arranged for obtaining optical spectra of the analytes adjacent to the SERS-active material (610a, 610b) in each of the second regions of the porous filter.
  • E8.A database comprising at least one predetermined optical spectrum of an analyte, wherein the predetermined optical spectrum is determined using a system according to embodiment El.
  • a database such as a database according to embodiment E8, comprising at least one predetermined optical spectrum of an analyte.
  • the porous filter being arranged so that the fluid sample may be placed onto a first region of the porous filter
  • SERS-active material 610a, 610b
  • the SERS active material has a SERS-active surface being placed at least partially within the pores of the porous filter within a second region of the porous filter, so that the first region and the second region of the porous filter are spatially separated and connected by through-going pores so that only sufficiently small analytes are able to reach the second region, and
  • wavelength selecting means such as at least one optical filter (638),
  • a method according to embodiment E10 for providing a system for performing optical spectroscopy of analytes in a fluid sample, wherein the step of placing a SERS-active material on the porous filter, comprises the steps of
  • step of placing a SERS-active material on the porous filter comprises the step of
  • a method according to embodiment E12, for providing a system for performing optical spectroscopy of analytes in a fluid sample, wherein the step of placing nanoparticles within the pores of the second region of the porous filter, comprises any one of the steps of:

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Abstract

La présente invention concerne un système d'obtention de spectre optique 648 d'analytes dans un échantillon fluide, un filtre poreux 602a, 602b étant agencé de telle sorte que l'échantillon fluide peut être placé sur une première région du filtre poreux, et une matière active en SERS (spectroscopie Raman exaltée de surface) 610a, 610b possédant une surface active en SERS étant placée au moins partiellement à l'intérieur des pores du filtre poreux à l'intérieur d'une deuxième région du filtre poreux. La première région et la deuxième région du filtre poreux sont séparées spatialement et reliées par des pores traversants de telle sorte qu'uniquement des analytes suffisamment petits sont aptes à atteindre la seconde région. Ainsi, le filtre poreux permet que l'échantillon fluide soit filtré de telle sorte qu'uniquement des entités suffisamment petites dans l'échantillon fluide atteignent la deuxième région où elles peuvent être sondées de telle sorte qu'un spectre optique associé aux analytes dans l'échantillon filtré peut être obtenu. Le système optique comprend également une source lumineuse 634, un détecteur de lumière 642, et le système d'analyse optique est conçu pour obtenir exclusivement de la deuxième région du filtre poreux 602a, 602b le spectre optique 648 des analytes adjacents à la matière active en SERS 610a, 610b.
PCT/DK2013/050157 2012-05-23 2013-05-23 Système d'obtention d'un spectre optique WO2013174387A1 (fr)

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WO2021209646A1 (fr) * 2020-04-17 2021-10-21 Universiteit Gent Guide d'ondes à phase de micro-extraction solide destiné à une spectroscopie raman

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102019122079A1 (de) * 2019-08-16 2021-02-18 Leibniz-Institut Für Polymerforschung Dresden E.V. Verfahren zur bestimmung von nanopolymerpartikeln
DE102019122079B4 (de) * 2019-08-16 2021-04-08 Leibniz-Institut Für Polymerforschung Dresden E.V. Verfahren zur bestimmung von nanopolymerpartikeln
WO2021209646A1 (fr) * 2020-04-17 2021-10-21 Universiteit Gent Guide d'ondes à phase de micro-extraction solide destiné à une spectroscopie raman

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