WO2013127280A1 - Production method and device for preventing and treating contamination of paraphysoderma sedebokerensis in haematococcus pluvialis - Google Patents
Production method and device for preventing and treating contamination of paraphysoderma sedebokerensis in haematococcus pluvialis Download PDFInfo
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Abstract
Provided is a method of preventing and controlling contamination in a scale culture of haematococcus pluvialis, comprising: 1) placing haematococcus pluvialis in a primary photo-bioreactor and cultured haematococcus pluvialis swarm cells; 2) transfering haematococcus pluvialis swarm cells to a secondary photo-bioreactor and adding a stress medium; 3) after more than 10% of the haematococcus pluvialis swarm cells in the secondary photo-bioreactor have changed to fixed cells, controlling and fixing the culturing temperature at 10-22°C so that haematococcus pluvialis spore cell algae fluid enters the astaxanthin accumulation period; and 4) after culturing for 7 - 12 days, obtaining haematococcus pluvialis spores with an infection rate of less than 1% of Paraphysoderma sedebokerensis. Also provided is a device for preventing and controlling contamination in a scale culture of haematococcus pluvialis. The device uses the waste heat of flue gases of a factory to provide energy for lowering the temperature of the haematococcus pluvialis algae fluid. The device can save on fuel costs, and reduce emissions of waste gases and harmful gases, thus protecting the environment.
Description
本发明涉及雨生红球藻的规模化培养中污染防控方法及装置，特别是涉及有效利用了现代工业所产生的余热，防治雨生红球藻中壶菌污染的生产方法与装置。
The invention relates to a pollution prevention and control method and device in large-scale cultivation of Haematococcus pluvialis, in particular to a production method and a device for effectively utilizing the waste heat generated by modern industry and controlling the contamination of the fungus of Haematococcus pluvialis.
天然虾青素具有强大的清除氧自由基的作用，抗氧化活性是类胡萝卜素的 10 倍，维生素 E 的 550
倍，被誉为超级抗氧化剂。雨生红球藻（ Haematococcus pluvialis ）在特定条件下可积累干物质的 1-3%
，誉为天然虾青素的浓缩品与最佳生物来源。而雨生红球藻培养时存在特异性壶菌污染，特别是规模化培养中，严重影响产品产量与品质，制约其应用规模。雨生红球藻具有特殊的生物学性质，其生活周期分为快速生长繁殖的游动细胞阶段和虾青素积累的不动细胞（孢子）阶段，这两个阶段所需的适宜培养条件相差较大。雨生红球藻游动细胞受盐胁迫后变为不动细胞，开始大量积累虾青素。收获后的雨生红球藻细胞中有
30% 的藻细胞被壶菌污染情况下，收获后 3 个月内天然虾青素含量（以干重计）可从 3% 降低到 0.5%
以下。采用密闭光生物反应器规模化生产时，一旦暴发流行时甚至造成雨生红球藻绝收。 Natural astaxanthin has a powerful role in scavenging oxygen free radicals. Its antioxidant activity is 10 times that of carotenoids and 550 times that of vitamin E. It is known as super antioxidant. Haematococcus pluvialis can accumulate 1-3% of dry matter under certain conditions, and is known as the concentrate and the best biological source of natural astaxanthin. However, there is a specific chytrid contamination during the culture of Haematococcus pluvialis, especially in large-scale culture, which seriously affects the yield and quality of the product and restricts its application scale. Haematococcus pluvialis has special biological properties, and its life cycle is divided into the rapid growth and reproduction of the swimming cell stage and the aspergillus accumulation of the immobile cells (spore) stage. The suitable culture conditions required for these two stages are different. Larger. The swimming cells of Haematococcus pluvialis turned into immobile cells after salt stress, and began to accumulate a large amount of astaxanthin. When 30% of the algae cells in the Haematococcus pluvialis cells are contaminated with chytrids, the natural astaxanthin content (by dry weight) can be reduced from 3% to less than 0.5% within 3 months after harvest. When large-scale production is carried out using a closed photobioreactor, the Haematococcus pluvialis is not harvested once the outbreak occurs.
侵染雨生红球藻的壶菌（ Paraphysoderma sedebokerensis
），是高等植物病原菌 Blastoclaidomycota 科 Physoderma 属的近缘种，通过水体传播。 Jenia Gutmana （
2009
）等证明该壶菌专一性寄生雨生红球藻，而不侵染绿藻纲的其它种类。侵染初期，无固定形态的壶菌侵染体附着在雨生红球藻不动孢子的表面，菌丝穿透细胞壁，吸取红球藻细胞质。壶菌菌丝体在雨生红球藻孢子内逐渐蔓延，并在雨生红球藻细胞外形成壶菌侵染体囊，并逐渐变大。侵染后期，雨生红球藻孢子颜色由红色逐渐变成黄色，最终降解为一团透明松散的细胞残体。壶菌的侵染体囊离开寄主，释放出更多的壶菌侵染体。在不利环境下，壶菌侵染体侵染后期会形成厚壁不动孢子囊，通过空气、土壤与水体传播，遇合适条件重新萌发并开始侵染。
Paraphysoderma sedebokerensis , which infects Haematococcus pluvialis, is a close relative of the genus Physoderma of the higher plant pathogen, Blastolaidomycota, which is transmitted through water bodies. Jenia Gutmana (2009) et al. demonstrated that the chytrid-specific parasitic Haematococcus pluvialis did not infect other species of the green algae class. In the early stage of infection, the infective body of the bacterium without immobilized form adhered to the surface of the non-invasive spores of Haematococcus pluvialis. The hyphae penetrated the cell wall and absorbed the cytoplasm of Haematococcus. The mycelium of the chymium gradually spreads in the spores of Haematococcus pluvialis, and forms a sac infecting the sac outside the cells of Haematococcus pluvialis, and gradually becomes larger. In the late stage of infection, the color of Haematococcus pluvialis spore changed from red to yellow, and eventually degraded into a group of transparent loose cell debris. The infective body capsule of the chytrid leaves the host and releases more of the chymophilic infecting body. In the unfavorable environment, the thick-walled non-spore sacs will form in the late stage of infection by the bacterium, and will spread through the air, soil and water, and re-emerge and start infestation under suitable conditions.
此外，节能与环保是当代全球关注的重要课题，我国是最大的发展中国家，按人口平均计算也是能源最匮乏的国家，我国装备工业较落后，能源利用率还较低，如化工、石油、建材、轻纺、冶金、动力、食品、造纸、电子电器等行业生产中大量的可利用热能直接排空，排放出二氧化碳以及各种有害气体，既浪费能源又污染环境。
In addition, energy conservation and environmental protection are important issues of global concern. China is the largest developing country. According to the average population, it is also the country with the most energy shortage. China's equipment industry is relatively backward, and energy utilization is still low, such as chemical and petroleum. In the production of building materials, textiles, metallurgy, power, food, paper, electronics and other industries, a large amount of available heat can be directly emptied, emitting carbon dioxide and various harmful gases, which wastes energy and pollutes the environment.
本发明的第一目的在于解决上述存在的问题，而提供一种能够有效降低雨生红球藻中壶菌的感染率，并在规模化培养中，提高产品产量与品质的雨生红球藻生产方法。
The first object of the present invention is to solve the above problems, and to provide a Haematococcus pluvialis which can effectively reduce the infection rate of the bacterium of Haematococcus pluvialis and improve the yield and quality of the product in large-scale cultivation. production method.
本发明的第二目的在于，提供一种节约能源、保护能源资源、保护环境，并能够规模化培养的防治雨生红球藻中壶菌污染的生产装置。
A second object of the present invention is to provide a production apparatus for preventing and controlling the contamination of the bacterium of Haematococcus pluvialis which can save energy, protect energy resources, protect the environment, and can be cultured in a large scale.
为了实现上述的第一目的，本发明提供的防治雨生红球藻中壶菌污染的生产方法，包括以下步骤： In order to achieve the above first object, the present invention provides a method for controlling the contamination of the chytrid in Haematococcus pluvialis, comprising the following steps:
①、将雨生红球藻置于一级光生物反应器中培养出的雨生红球藻游动细胞； 1. The Haematococcus pluvialis swimming cells cultured in a primary photobioreactor by Haematococcus pluvialis;
②、将雨生红球藻游动细胞转入二级光生物反应器，加入胁迫培养基； 2. Transfer the swimming cells of Haematococcus pluvialis into a secondary photobioreactor and add the stress medium;
③、二级光生物反应器中 10%
以上的雨生红球藻游动细胞转化为不动细胞后，将进入虾青素积累阶段的雨生红球藻孢子细胞藻液的温度控制在 10 ～ 22 ℃培养； 3. 10% in the secondary photobioreactor
After the above-mentioned Haematococcus pluvialis swimming cells are transformed into immobile cells, the temperature of the algae solution of Haematococcus pluvialis spore cells entering the astaxanthin accumulation stage is controlled at 10 to 22 ° C;
④、 培养 7 ～ 12 天后，收获雨生红球藻孢子。 4. After 7 to 12 days of cultivation, the spores of Haematococcus pluvialis are harvested.
较佳地，在步骤①中，所述雨生红球藻游动细胞比例为 80 ～ 100% 。 Preferably, in step 1, the proportion of swimming cells of Haematococcus pluvialis is 80 to 100%.
较佳地，在步骤①中，所述雨生红球藻游动细胞比例为 96% 。 Preferably, in step 1, the proportion of swimming cells of Haematococcus pluvialis is 96%.
在上述技术方案中，在步骤②中，所述胁迫培养基成分为氯化钠，所述浓度为 0.1 ～ 1g/L
。 In the above technical solution, in the step 2, the stress medium component is sodium chloride, and the concentration is 0.1 to 1 g/L.
.
较佳地，在步骤③中，所述二级光生物反应器中雨生红球藻游动细胞转化为不动细胞的比例为 60 ～
90% 。 Preferably, in step 3, the ratio of the swimming cells of Haematococcus pluvialis to the immobile cells in the secondary photobioreactor is 60 ～
90%.
较佳地，在步骤③中，所述将进入虾青素积累阶段的雨生红球藻孢子细胞藻液的温度控制在 16 ℃培养
。
Preferably, in step 3, the temperature of the Haematococcus pluvialis spore cell algae liquid entering the astaxanthin accumulation stage is controlled to be cultured at 16 °C .
为了实现本发明的第二目的，本发明提供的防治雨生红球藻中壶菌污染的生产装置，包括一级光生物反应器和二级光生物反应器，所述二级光生物反应器通过管道连接有热交换器，所述热交换器的输入端通过管道连接有冷凝器，输出端通过管道连接有吸收器，所述吸收器的输入端通过管道和溶液泵与发生器连接，输出端通过管道和节流阀与发生器连接，所述发生器的输出端通过管道与所述冷凝器连接，所述发生器输入端通过管道与水箱连接，所述水箱通过余热回收器连接带有废热的烟气。
In order to achieve the second object of the present invention, the present invention provides a production apparatus for controlling the contamination of the bacterium of Haematococcus pluvialis, comprising a primary photobioreactor and a secondary photobioreactor, the secondary photobioreactor A heat exchanger is connected through a pipe, the input end of the heat exchanger is connected to the condenser through a pipe, the output end is connected to the absorber through a pipe, and the input end of the absorber is connected to the generator through the pipe and the solution pump, and the output is output. The end is connected to the generator via a pipe and a throttle valve, the output of which is connected to the condenser via a pipe, the generator input being connected to the water tank via a pipe, the water tank being connected by a waste heat recovery device Waste heat fumes.
进一步地，所述带有废热的烟气为电厂带废热的烟气或石化燃料电厂带废热的烟气或生物质电厂带废热的烟气。
Further, the flue gas with waste heat is flue gas with waste heat of a power plant or flue gas with waste heat of a petrochemical fuel power plant or flue gas with waste heat of a biomass power plant.
进一步地，所述发生器和吸收器中的介质为具有在低压下吸收水蒸气，并在高压下产生水蒸气的盐类溶液，如溴化锂溶液。
Further, the medium in the generator and the absorber is a salt solution having a water vapor absorption at a low pressure and generating water vapor under a high pressure, such as a lithium bromide solution.
本发明的生产方法，控制雨生红球藻的生产过程中的各项参数和生产条件，试验证明在雨生红球藻培养到第
11 天的壶菌感染率最高值为 0.5%
，大大降低了壶菌感染率，为规模化生产雨生红球藻提开辟了可行的道路。并在实验室内，控制培养温度条件，测量温度对壶菌侵染的影响，测量结果参阅图 1
所示，其培养的具体条件是，雨生红球藻孢子细胞密度为 15 × 104 个 /ml ，接种 50ml 用 CGM 培养基的壶菌培养液。分别在 16 ℃， 20
℃， 25 ℃， 32 ℃下培养，每天监测培养状况，记录壶菌的侵染率（雨生红球藻的感染率），结果详见下图。接种较高浓度的壶菌侵染体， 32 ℃下第 5
天侵染率已近 90% ， 25 ℃下第 8 天的侵染率达到 90% 以上，均认为绝收； 16 ℃下雨生红球藻的感染率培养到第 11 天最高仅为到 0.5% 。
The production method of the invention controls various parameters and production conditions in the production process of Haematococcus pluvialis, and the test proves that the cultivation of Haematococcus pluvialis is up to the first
The highest rate of chytrid infection in 11 days was 0.5%
, greatly reduced the infection rate of chytrid, and opened up a feasible road for the large-scale production of Haematococcus pluvialis. And in the laboratory, control the culture temperature conditions, measure the effect of temperature on the infection of the chytrid, the measurement results refer to Figure 1
As shown, the specific conditions for the culture were as follows: the spore cell density of Haematococcus pluvialis was 15 × 104 cells/ml, and 50 ml of the chytrid culture medium using CGM medium was inoculated. At 16 °C, 20
Incubate at °C, 25 °C, 32 °C, monitor the culture status every day, and record the infection rate of H. pneumoniae (infection rate of Haematococcus pluvialis). The results are shown in the figure below. Inoculate a higher concentration of the chytrid infestation, 5 at 32 °C
The infection rate is nearly 90%, and the infection rate on the 8th day at 25 °C is over 90%, which is considered to be unsuccessful. The infection rate of Haematococcus pluvialis cultured at 16 °C is up to 0.5% on the 11th day. .
本发明的生产装置利用工厂烟气余热提供的能量控制雨生红球藻生产当中存在的壶菌污染，余热是尚能利用而未被利用的热能，不仅节省了燃料费用，提高了经济效益，增强了企业竞争力，而且大大减少了废气、有害气体的排放，保护环境。
The production device of the invention utilizes the energy provided by the waste heat of the factory flue gas to control the contamination of the chytrid in the production of Haematococcus pluvialis, and the residual heat is the heat energy that can be utilized but not utilized, which not only saves fuel costs, but also improves economic benefits. It enhances the competitiveness of enterprises and greatly reduces emissions of harmful gases and harmful gases and protects the environment.
图 1 为本发明分别在 16 ℃， 20 ℃， 25 ℃， 32
℃下培养雨生红球藻，壶菌污染试验对比图；图 1 中，——表示 16 ℃温度下培养雨生红球藻，壶菌浸染率；- . -表示 20
℃温度下培养雨生红球藻，壶菌浸染率； ……. 表示 25 ℃温度下培养雨生红球藻，壶菌浸染率； ------ 表示 32
℃温度下培养雨生红球藻，壶菌浸染率；横坐标为培养的天数，纵坐标为浸染率。Fig. 1 is a comparison chart of the test of the contamination of the genus Haematococcus in the culture of Haematococcus pluvialis at 16 °C, 20 °C, 25 °C and 32 °C, respectively; Figure 1 shows that the culture of Haematococcus pluvialis at 16 °C , the rate of chymosis infusion; - . - indicates the culture rate of Haematococcus pluvialis cultured at 20 °C, and the rate of chymophila soaking; ....... indicates the culture rate of Haematococcus pluvialis at 25 °C; -- Indicates the rate of culture of Haematococcus pluvialis cultured at 32 °C; the abscissa is the number of days of culture, and the ordinate is the rate of infiltration.
图2为本发明装置的结构示意图； Figure 2 is a schematic structural view of the device of the present invention;
图中： 1 、二级光生物反应器； 2 、热交换器； 3 、冷凝器； 4 、吸收器； 5 、溶液泵； 6
、发生器； 7 、节流阀； 8 、一级光生物反应器； 9 、水箱； 10 、余热回收器； 11 、胁迫培养基； 12 、工业废气。 In the figure: 1, secondary photobioreactor; 2, heat exchanger; 3, condenser; 4, absorber; 5, solution pump;
, generator; 7, throttle valve; 8, first-level photobioreactor; 9, water tank; 10, waste heat recovery; 11, stress medium; 12, industrial waste gas.
下面结合实施例和附图对本发明作进一步的详细说明。 The present invention will be further described in detail below with reference to the embodiments and the accompanying drawings.
实施例一 ： Embodiment 1 :
防治雨生红球藻中壶菌污染的生产方法，其步骤为：①、将雨生红球藻置于一级光生物反应器中培养出的雨生红球藻游动细胞；雨生红球藻游动细胞比例为 96%
；②、将雨生红球藻游动细胞转入二级光生物反应器，加入胁迫培养基，胁迫培养基主要成分为浓度为 0.5 g/L ③、二级光生物反应器中 60%
的雨生红球藻游动细胞转化为不动细胞后，将进入虾青素积累阶段的雨生红球藻孢子细胞藻液的温度控制在 16 ℃培养；④、培养 11
天后，收获雨生红球藻孢子，镜检雨生红球藻的壶菌感染率为 0.4% ，天然虾青素含量为 4.8% （以干重计）。
The production method for controlling the contamination of the fungus of Haematococcus pluvialis, the steps are as follows: 1. The Haematococcus pluvialis swimming cells cultured in the first photobioreactor; the rain red The proportion of swimming cells in the algae is 96%
2, the Haematococcus pluvialis swimming cells were transferred to the secondary photobioreactor, and the stress medium was added. The main component of the stress medium was 0.5 g/L 3 and 60% in the secondary photobioreactor.
After the Haematococcus pluvialis swimming cells are transformed into immobile cells, the temperature of the Haematococcus pluvialis spore cell into the astaxanthin accumulation stage is controlled at 16 ° C; 4, culture 11
After the day, the spores of Haematococcus pluvialis were harvested, and the prevalence of H. pneumoniae was 0.4% and the natural astaxanthin content was 4.8% (dry weight).
实施例二： Embodiment 2:
防治雨生红球藻中壶菌污染的生产方法，其步骤为：①、将雨生红球藻置于一级光生物反应器中培养出的雨生红球藻游动细胞；雨生红球藻游动细胞比例为 80%
；②、将雨生红球藻游动细胞转入二级光生物反应器，加入胁迫培养基，胁迫培养基主要成分为浓度为 0.1 g/L ③、二级光生物反应器中 90%
的雨生红球藻游动细胞转化为不动细胞后，将进入虾青素积累阶段的雨生红球藻孢子细胞藻液的温度控制在 10 ℃培养； ④、 培养 9
天后，收获雨生红球藻孢子。镜检雨生红球藻的壶菌感染率为 0.2% ，天然虾青素含量为 3.8% （以干重计）。
The production method for controlling the contamination of the fungus of Haematococcus pluvialis, the steps are as follows: 1. The Haematococcus pluvialis swimming cells cultured in the first photobioreactor; the rain red The proportion of swimming cells in the algae is 80%
2, the Haematococcus pluvialis swimming cells were transferred to the secondary photobioreactor, and the stress medium was added. The main component of the stress medium was 0.1 g/L 3 and 90% in the secondary photobioreactor.
After the Haematococcus pluvialis swimming cells are transformed into immobile cells, the temperature of the Haematococcus pluvialis spore cell into the astaxanthin accumulation stage is controlled at 10 °C; 4. Culture 9
After the day, harvest the Haematococcus spores. The microscopic examination of Haematococcus pluvialis had a bacterium infection rate of 0.2% and a natural astaxanthin content of 3.8% (by dry weight).
实施例三： Embodiment 3:
防治雨生红球藻中壶菌污染的生产方法，其步骤为：①、将雨生红球藻置于一级光生物反应器中培养出的雨生红球藻游动细胞；雨生红球藻游动细胞比例为 100%
；②、将雨生红球藻游动细胞转入二级光生物反应器，加入胁迫培养基，胁迫培养基主要成分为浓度为 1 g/L ③、二级光生物反应器中 90%
的雨生红球藻游动细胞转化为不动细胞后，将进入虾青素积累阶段的雨生红球藻孢子细胞藻液的温度控制在 18 ℃培养； ④、 培养 12
天后，收获雨生红球藻孢子。镜检雨生红球藻的壶菌感染率为 0.5% ，天然虾青素含量为 5.6% （以干重计）。
The production method for controlling the contamination of the fungus of Haematococcus pluvialis, the steps are as follows: 1. The Haematococcus pluvialis swimming cells cultured in the first photobioreactor; the rain red The proportion of swimming cells in the algae is 100%
2, the Haematococcus pluvialis swimming cells were transferred to the secondary photobioreactor, and the stress medium was added. The main component of the stress medium was 1 g/L 3 and 90% in the secondary photobioreactor.
After the Haematococcus pluvialis swimming cells are transformed into immobile cells, the temperature of the Haematococcus pluvialis spore cell into the astaxanthin accumulation stage is controlled at 18 ° C; 4. Culture 12
After the day, harvest the Haematococcus spores. The microscopic examination of Haematococcus pluvialis was 0.5% and the natural astaxanthin content was 5.6% (by dry weight).
实施例四： Embodiment 4:
防治雨生红球藻中壶菌污染的生产方法，其步骤为：①、将雨生红球藻置于一级光生物反应器中培养出的雨生红球藻游动细胞；雨生红球藻游动细胞比例为 90%
；②、将雨生红球藻游动细胞转入二级光生物反应器，加入胁迫培养基，胁迫培养基主要成分为浓度为 0.6 g/L ③、二级光生物反应器中 60%
的雨生红球藻游动细胞转化为不动细胞后，将进入虾青素积累阶段的雨生红球藻孢子细胞藻液的温度控制在 16 ℃培养； ④、 培养 7
天后，收获雨生红球藻孢子。镜检雨生红球藻的壶菌感染率为 0.6% ，天然虾青素含量为 5.9% （以干重计）。
The production method for controlling the contamination of the fungus of Haematococcus pluvialis, the steps are as follows: 1. The Haematococcus pluvialis swimming cells cultured in the first photobioreactor; the rain red The proportion of swimming cells in the algae is 90%
2, the Haematococcus pluvialis swimming cells were transferred to a secondary photobioreactor, and the stress medium was added. The main component of the stress medium was 0.6 g/L 3 and 60% in the secondary photobioreactor.
After the Haematococcus pluvialis swimming cells are transformed into immobile cells, the temperature of the Haematococcus pluvialis spore cell into the astaxanthin accumulation stage is controlled at 16 ° C; 4. Culture 7
After the day, harvest the Haematococcus spores. The microscopic examination of Haematococcus pluvialis had a prevalence of 0.6% and natural astaxanthin content of 5.9% (by dry weight).
通过上述四个实施例看出，采用本发明的方法培养出的雨生红球藻中壶菌污染率降至 0.2% - 0.6%
，如图 1 所示的雨生红球藻在不同温度条件下培养的壶菌污染率示意图，将进入虾青素积累阶段的雨生红球藻孢子细胞藻液的温度控制在 16
℃培养，壶菌污染率明显低于在 20 ℃、 25 ℃、 32 ℃的温度条件下培养。 It can be seen from the above four examples that the contamination rate of the bacterium in Haematococcus pluvialis cultured by the method of the present invention is reduced to 0.2% - 0.6%.
As shown in Figure 1, the Schizophrenia contamination rate of Haematococcus pluvialis cultured under different temperature conditions, the temperature of the algae solution of Haematococcus pluvialis spore cells entering the astaxanthin accumulation stage is controlled at 16
In the °C culture, the contamination rate of the fungus was significantly lower than that at 20 °C, 25 °C, and 32 °C.
实施例五： Embodiment 5:
如图 2 所示，防治雨生红球藻中壶菌污染的生产装置，包括一级光生物反应器 8 和二级光生物反应器 1
，二级光生物反应器 1 通过管道连接有热交换器 2 ，热交换器 2 的输入端通过管道连接有冷凝器 3 ，输出端通过管道连接有吸收器 4 ，吸收器 4
的输入端通过管道和溶液泵 5 与发生器 6 连接，输出端通过管道和节流阀 7 与发生器 6 连接，发生器 6 的输出端通过管道与冷凝器 3 连接，发生器 6
输入端通过管道与水箱 9 连接，水箱 9 通过余热回收器 10 连接带有废热的烟气。 As shown in Fig. 2, a production device for controlling the contamination of the bacterium of Haematococcus pluvialis, including the primary photobioreactor 8 and the secondary photobioreactor 1
, the secondary photobioreactor 1 is connected to the heat exchanger 2 through a pipeline, the input end of the heat exchanger 2 is connected to the condenser 3 through a pipeline, and the output end is connected to the absorber 4 through a pipeline, and the absorber 4
The input is connected to the generator 6 via a pipe and solution pump 5, the output is connected to the generator 6 via a pipe and a throttle valve 7, and the output of the generator 6 is connected to the condenser 3 via a pipe, the generator 6
The input is connected to the water tank 9 through a pipe, and the water tank 9 is connected to the flue gas with waste heat through the waste heat recovery unit 10.
带有废热的烟气为电厂带废热的烟气或石化燃料电厂带废热的烟气或生物质电厂带废热的烟气，给热交换器 2
提供动力。发生器 6 中设有溴化锂制冷剂，或其它具有强水蒸气吸收能力的物质。发生器 6 和吸收器 4
中的介质为具有在低压下吸收水蒸气，并在高压下产生水蒸气的盐类溶液，如溴化锂溶液。 The flue gas with waste heat is flue gas with waste heat from the power plant or flue gas with waste heat from the petrochemical fuel power plant or flue gas with waste heat from the biomass power plant to the heat exchanger 2
Provide power. The generator 6 is provided with a lithium bromide refrigerant or other substance having a strong water vapor absorbing ability. Generator 6 and absorber 4
The medium in the medium is a salt solution having a water vapor absorption at a low pressure and generating water vapor under a high pressure, such as a lithium bromide solution.
其工作过程如下：将雨生红球藻置于一级光生物反应器 8
中培养出的雨生红球藻游动细胞；将雨生红球藻游动细胞转入二级光生物反应器 1 ，加入胁迫培养基 11 ；将二级光生物反应器 1
中的雨生红球藻藻液通过管道通入热交换器 2 降温，通过热交换器 2 将藻液培养温度从 30 ℃左右（或 30 ℃以上）降低至 16 ℃，热交换器 2
的能量来源为电厂烟气废热或其它热能。将电厂烟气经过管道输入到余热回收器 10 ，加热常温热源软水至 98 ℃，通入发生器 6 放出汽化潜热，发生器 6
当中的制冷剂溶液水分被热源蒸汽蒸发，制冷剂溶液浓度增大，发生器 6 中从制冷剂溶液中蒸发出的水蒸气向上经挡板进入冷凝器 3
，防止液滴随蒸汽进入冷凝器。热源蒸汽压力为 0.050 ～ 0.500Mpa ，优选 0.010Mpa
。通过制冷剂产生高压冷剂水蒸气，高压冷剂水蒸气通入冷凝器 3 中变为高压液态冷剂水，通过节流阀 7 后变为低压冷剂水蒸气，进入热交换器 2
吸热变为低压冷剂液态水，低压冷剂液态水进入吸收器 4 释放热量，然后泵回发生器 6 。热源水凝结后回收至余热回收器 10 加热提供热源，余热回收器 10
接收来自工业废气 12 中热量。 The working process is as follows: Haematococcus pluvialis is placed in the primary photobioreactor 8
The Haematococcus pluvialis swimming cells are cultured; the Haematococcus pluvialis swimming cells are transferred to the secondary photobioreactor 1 , the stress medium 11 is added; and the secondary photobioreactor 1
The Haematococcus pluvialis algae liquid is piped into the heat exchanger 2 to cool down, and the algae culture temperature is lowered from 30 °C (or above 30 °C) to 16 °C through the heat exchanger 2, the heat exchanger 2
The source of energy is power plant flue gas waste heat or other thermal energy. The power plant flue gas is input into the waste heat recovery device 10 through the pipeline, and the normal temperature heat source soft water is heated to 98 ° C, and the generator 6 is introduced to release the latent heat of vaporization, and the generator 6
The moisture of the refrigerant solution is evaporated by the heat source vapor, and the concentration of the refrigerant solution is increased, and the water vapor evaporated from the refrigerant solution in the generator 6 is directed upward through the baffle into the condenser 3
To prevent droplets from entering the condenser with steam. The heat source steam pressure is 0.050 ~ 0.500Mpa, preferably 0.010Mpa
. The high-pressure refrigerant water vapor is generated by the refrigerant, and the high-pressure refrigerant water vapor is passed into the condenser 3 to become the high-pressure liquid refrigerant water, which passes through the throttle valve 7 and becomes the low-pressure refrigerant water vapor, and enters the heat exchanger 2
The heat absorption becomes the low pressure refrigerant liquid water, and the low pressure refrigerant liquid water enters the absorber 4 to release the heat, and then is pumped back to the generator 6 . The heat source water is condensed and recovered to the waste heat recovery unit. 10 Heating to provide heat source, waste heat recovery unit 10
Receive heat from industrial waste gas 12.
显然，本领域的技术人员可以对本发明进行各种改动和变型而不脱离本发明的精神和范围。这样，倘若本发明的这些修改和变型属于本发明权利要求及其等同技术的范围之内，则本发明也意图包含这些改动和变型在内。
It is apparent that those skilled in the art can make various modifications and variations to the invention without departing from the spirit and scope of the invention. Thus, it is intended that the present invention cover the modifications and modifications of the invention
本说明书中未作详细描述的内容属于本领域专业技术人员公知的现有技术 。 The contents not described in detail in the present specification belong to the prior art well known to those skilled in the art.
Claims (9)
1 、一种防治雨生红球藻中壶菌污染的生产方法，其特征在于包括以下步骤： 1 . A production method for controlling the contamination of chytrids in Haematococcus pluvialis, characterized in that the method comprises the following steps:
①、将雨生红球藻置于一级光生物反应器中培养出的雨生红球藻游动细胞；1. The Haematococcus pluvialis swimming cells cultured in a primary photobioreactor by Haematococcus pluvialis;
②、将雨生红球藻游动细胞转入二级光生物反应器，加入胁迫培养基；2. Transfer the swimming cells of Haematococcus pluvialis into a secondary photobioreactor and add the stress medium;
③、二级光生物反应器中雨生红球藻细胞总数中不动细胞比例大于 10%
后，将进入虾青素积累阶段的雨生红球藻孢子细胞藻液的温度控制在 10 ～ 22 ℃培养；3. The proportion of immobile cells in the total number of Haematococcus pluvialis cells in the secondary photobioreactor is greater than 10%.
Thereafter, the temperature of the Haematococcus pluvialis spore cell algae solution entering the astaxanthin accumulation stage is controlled to be cultured at 10 to 22 ° C;
④、 培养 7 ～ 12 天后，收获雨生红球藻孢子。4. After 7 to 12 days of cultivation, the spores of Haematococcus pluvialis are harvested.
2 、根据权利要求 1
所述的防治雨生红球藻中壶菌污染的生产方法，其特征在于：在步骤①中，所述雨生红球藻游动细胞数量占全部雨生红球藻细胞的 80 ～ 100% 。
2, according to claim 1
The method for producing the fungus contamination of Haematococcus pluvialis is characterized in that: in step 1, the number of swimming cells of Haematococcus pluvialis accounts for 80 to 100% of all Haematococcus pluvialis cells. .
3 、根据权利要求 2
所述的防治雨生红球藻中壶菌污染的生产方法，其特征在于：在步骤①中，所述雨生红球藻游动细胞比例占全部雨生红球藻细胞的 96% 。 3, according to claim 2
The method for producing the fungus contamination of Haematococcus pluvialis is characterized in that, in step 1, the proportion of swimming cells of Haematococcus pluvialis accounts for 96% of all Haematococcus pluvialis cells.
4 、 根据权利要求 1
所述的防治雨生红球藻中壶菌污染的生产方法，其特征在于：在步骤②中，所述胁迫培养基成分为氯化钠水溶液，所述溶液在培养藻液中的终浓度为 0.1 ～ 10g/L 。
4, according to claim 1
The method for producing the fungus contamination of Haematococcus pluvialis is characterized in that, in step 2, the stress medium component is an aqueous solution of sodium chloride, and the final concentration of the solution in the culture algae solution is 0.1 to 10g/L.
5 、 根据权利要求 1
所述的防治雨生红球藻中壶菌污染的生产方法，其特征在于：在步骤③中，所述二级光生物反应器中雨生红球藻不动细胞数量占全部雨生红球藻细胞的 60 ～ 90% 。
5, according to claim 1
The method for producing the fungus contamination of Haematococcus pluvialis is characterized in that: in step 3, the number of immobile cells of Haematococcus pluvialis in the secondary photobioreactor accounts for all rainy red balls 60 to 90% of algae cells.
6 、根据权利要求 1
所述的防治雨生红球藻中壶菌污染的生产方法，其特征在于：在步骤③中，所述将进入虾青素积累阶段的雨生红球藻孢子细胞藻液的温度控制在 16 ℃培养。
6. According to claim 1
The method for producing the fungus contamination of Haematococcus pluvialis is characterized in that, in step 3, the temperature of the algae solution of Haematococcus pluvialis spore cells entering the astaxanthin accumulation stage is controlled at 16 Culture at °C.
7
、一种防治雨生红球藻中壶菌污染的生产装置，其特征在于：包括一级光生物反应器和二级光生物反应器，所述二级光生物反应器通过管道连接有热交换器，所述热交换器的输入端通过管道连接有冷凝器，输出端通过管道连接有吸收器，所述吸收器的输入端通过管道和溶液泵与发生器连接，输出端通过管道和节流阀与发生器连接，所述发生器的输出端通过管道与所述冷凝器连接，所述发生器输入端通过管道与水箱连接，所述水箱通过余热回收器连接带有废热的烟气。7
a production device for controlling the contamination of the bacterium of Haematococcus pluvialis, characterized in that it comprises a first-stage photobioreactor and a secondary photobioreactor, wherein the secondary photobioreactor is connected to the heat exchange through a pipeline The input end of the heat exchanger is connected to the condenser through a pipe, and the output end is connected to the absorber through a pipe. The input end of the absorber is connected to the generator through a pipe and a solution pump, and the output end is piped and throttled. The valve is connected to a generator, the output of which is connected to the condenser via a pipe, the generator input being connected to the water tank via a pipe, the water tank connecting the flue gas with waste heat through a waste heat recovery device.
8 、根据权利要求 7
所述的防治雨生红球藻中壶菌污染的生产装置，其特征在于：所述带有废热的烟气为电厂带废热的烟气或石化燃料电厂带废热的烟气或生物质电厂带废热的烟气。
8. According to claim 7
The production device for controlling the contamination of the bacterium of Haematococcus pluvialis is characterized in that: the flue gas with waste heat is flue gas of waste heat of power plant or flue gas or biomass power plant belt with waste heat of petrochemical fuel power plant Waste heat fumes.
9 、根据权利要求 7
所述的防治雨生红球藻中壶菌污染的生产装置，其特征在于：所述发生器和吸收器中的介质为具有在低压下吸收水蒸气，并在高压下产生水蒸气的盐类溶液。
9. According to claim 7
The production device for controlling the contamination of the fungus of Haematococcus pluvialis is characterized in that the medium in the generator and the absorber is a salt having water vapor at a low pressure and generating water vapor under a high pressure. Solution.
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| WO2016154174A1 (en) * | 2015-03-25 | 2016-09-29 | Heliae Development Llc | Methods of culturing haematococcus |
| CN109971649A (en) * | 2019-04-28 | 2019-07-05 | 哈尔滨师范大学 | It is a kind of for cultivating the culture medium and intelligent checking system of haematococcus pluvialis |
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| CN106701585B (en) * | 2015-07-23 | 2020-05-19 | 中国石油化工股份有限公司 | Culture method of haematococcus pluvialis |
| CN107502646A (en) * | 2017-10-11 | 2017-12-22 | 广西南宁秀珀生物科技有限公司 | Utilize the method for patter of rain haematococcus efficient accumulation astaxanthin |
| CN110881463B (en) * | 2018-09-07 | 2021-12-03 | 中国科学院武汉植物园 | Method for preventing and treating fungal infection in haematococcus culture by using surfactant |
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| CN102604836A (en) | 2012-07-25 |
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