WO2013105204A1 - 検体濃縮容器とそれを用いた検体濃縮方法 - Google Patents
検体濃縮容器とそれを用いた検体濃縮方法 Download PDFInfo
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- WO2013105204A1 WO2013105204A1 PCT/JP2012/008391 JP2012008391W WO2013105204A1 WO 2013105204 A1 WO2013105204 A1 WO 2013105204A1 JP 2012008391 W JP2012008391 W JP 2012008391W WO 2013105204 A1 WO2013105204 A1 WO 2013105204A1
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- sample
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- lid
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- concentration
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
- B01L3/50215—Test tubes specially adapted for centrifugation purposes using a float to separate phases
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0848—Specific forms of parts of containers
- B01L2300/0851—Bottom walls
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
- B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0605—Valves, specific forms thereof check valves
- B01L2400/0616—Ball valves
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0633—Valves, specific forms thereof with moving parts
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4083—Concentrating samples by other techniques involving separation of suspended solids sedimentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
Definitions
- the present invention relates to a specimen concentration container and a specimen concentration method using the same.
- the sample concentrator disclosed in Patent Document 1 is provided with a sample concentrating portion on the bottom surface thereof.
- the specimen concentrating portion is a small tube having an inner diameter smaller than that of the other part (the main part of the specimen concentrating container).
- the liquid mixture in the container body evaporates from the upper surface opening formed on the upper surface side of the container body. Thereby, the liquid mixture in a container main body is concentrated.
- the mixed solution reaches a certain amount, the amount of the sample present in the mixed solution in this state is measured.
- the bottom portion of the container main body is provided with a capillary-shaped sample concentrator having an inner diameter smaller than the inner diameter of the container main body.
- the sample concentration device of the above-described conventional example when the liquid level of the mixed solution reaches the sample concentration unit, the amount of evaporation thereafter decreases rapidly, so that an almost constant amount can be obtained without constant observation.
- the mixed solution can be left in the specimen concentration part.
- the upper surface is open, so that if the sample concentrating portion is left for a long period of time, the liquid mixture is surely evaporated from the sample concentrating portion, and as a result, constant There may be a case where an amount of the mixed liquid cannot be left in the specimen concentration unit. For this reason, the operator has to monitor the amount of the liquid mixture in the container body constantly or repeatedly, and the work efficiency is extremely low.
- the present invention has been made in view of the above-described problems, and an object of the present invention is to realize a specimen concentration container that can increase the working efficiency.
- a sample concentration container is a sample concentration container in which a mixed solution containing a sample is accommodated and the mixed solution is concentrated therein.
- a tube-shaped container main body having a portion, and a second opening for communicating with the inside of the container main body, the specimen concentrating unit for storing the concentrated mixed liquid formed on the bottom side of the container main body And a specimen lid for covering the second opening disposed in the container main body.
- the present invention is configured and has an effect of improving workability.
- the present invention provides the following aspects.
- a specimen concentration container is a specimen concentration container that contains a liquid mixture containing a specimen and in which the liquid mixture is concentrated, and a tube having a first opening on an upper surface thereof.
- a container main body having a shape, a second opening for communicating with the inside of the main body of the container, and formed on the bottom side of the main body of the container to store the concentrated mixed liquid, and the container A specimen lid for covering the second opening disposed in the body.
- the sample concentration container according to the first aspect of the present invention has an effect that the working efficiency can be increased.
- the inner diameter of the second opening is smaller than the inner diameter of the container body,
- the bottomed cylindrical shape in which the second opening is formed may be used.
- the sample concentrating portion has a bottomed cylindrical configuration in which the second opening having an inner diameter smaller than the inner diameter of the container body is formed on the upper portion.
- the amount of evaporation of the liquid mixture can be reduced when the sample is in the sample concentrating unit than when the sample is in the sample concentration unit.
- a liquid mixture can be accommodated in the sample concentration part.
- the sample concentration container according to a third aspect of the present invention is the sample concentration container according to the first aspect or the second aspect described above, wherein the sample lid has a liquid level of the mixed solution when the mixed solution is concentrated. It may be configured to cover the second opening when it is lower than the second opening of the part.
- the second opening can be covered when the liquid level of the mixed solution is lower than the second opening of the specimen concentration part. That is, the second opening can be covered with the sample lid at the timing when the mixed solution is stored in the sample concentration unit. For this reason, it can prevent that the liquid mixture accommodated in the specimen concentration part evaporates.
- the specimen lid in any one of the first to third aspects described above, has a diameter larger than the diameter of the second opening. It may be spherical. When the sample lid is formed in a spherical shape, the second opening can be covered on any surface of the sample lid.
- a sample concentration container includes a container main body having an upper surface opened, and a lid provided on the upper surface opening of the container main body so as to be openable and closable.
- a cup-shaped sample concentrating portion having an upper surface opened and an inner diameter smaller than the inner diameter of the container main body is provided, and the diameter of the upper surface opening of the sample concentrating portion is provided on the upper surface opening of the sample concentrating portion.
- a spherical specimen lid having a larger diameter is movably disposed.
- the lid since the lid is provided, it is possible to prevent the specimen lid or the like in the container body from coming out of the container body. Since the sample lid is disposed on the upper surface opening of the cup-shaped sample concentrating unit, the upper surface opening of the sample concentrating unit can be closed when the sample lid moves so as to cover the upper surface opening. For this reason, it is possible to prevent, for example, a mixed solution containing the specimen, for example, contained in the specimen concentration section from being released by evaporation or the like.
- the specimen lid may be made of a synthetic resin.
- the specimen lid may be made of a polypropylene resin.
- the specimen lid may be made of a polyethylene resin.
- the specimen concentration container according to the ninth aspect of the present invention is the specimen concentration container according to any one of the first to fifth aspects described above, wherein the specimen lid is configured to float in the mixed solution. Good.
- the sample lid since the sample lid is configured to float on the mixed solution, the mixed solution stored in the sample concentration container evaporates by concentration, and the mixed solution is stored in the sample concentration unit.
- the second opening which is the upper surface opening of the specimen concentrating part, can be closed at the timing when the amount is accommodated. For this reason, it can prevent that the liquid mixture in a sample concentration part evaporates, and can keep a fixed quantity of liquid mixture in this sample concentration part.
- the sample concentration container according to a tenth aspect of the present invention is the sample concentration container according to the third aspect described above, wherein the sample lid has the liquid level of the mixed solution when the mixed solution is concentrated.
- the sample lid When lower than the opening, it may be configured to cover the second opening by making surface contact with the container main body in the vicinity of the second opening.
- the sample lid is in surface contact with the container body in the vicinity of the second opening and covers the second opening, so that the second opening is firmly secured so that the liquid mixture in the sample concentrating portion does not evaporate. And can be closed.
- the specimen concentration container according to the eleventh aspect of the present invention is the ninth or tenth aspect described above, wherein the specimen lid may include a hollow portion therein. Since the hollow portion is included inside, the specific gravity of the specimen lid can be made smaller than that of the mixed solution. For this reason, the specimen lid can be suitably floated in the mixed solution.
- the specimen concentration container according to the twelfth aspect of the present invention may be configured such that, in the ninth or tenth aspect, the specimen lid is provided with a columnar projecting portion projecting in one direction.
- the sample concentration container according to the thirteenth aspect of the present invention may be configured such that the total length of the sample lid including the protruding portion is longer than the inner diameter of the container body. .
- the specimen concentration container according to a fourteenth aspect of the present invention is the specimen lid body according to the twelfth aspect, wherein the specimen lid includes a surface that closes the second opening, and the protrusion is formed. And at least the inside of the protruding portion may be hollow.
- the center of gravity of the specimen lid can be biased toward the specimen lid body.
- the specimen lid can be arranged in the container body of the specimen concentration container so that the specimen lid body is downward and the protruding portion is upward.
- the sample lid can cover the second opening portion on the surface opposite to the side on which the protruding portion on the sample lid main body side is formed.
- a specimen concentration method is a specimen concentration method using the specimen concentration container according to any one of the first to fourteenth aspects, wherein the specimen concentration container A mixture of the extract and the specimen is placed therein, and the specimen concentration container is rotated by a centrifuge under reduced pressure to generate centrifugal force to concentrate the mixture.
- the sample concentration method according to the fifteenth aspect of the present invention has an effect of improving the working efficiency.
- the sample concentration container 1 of the present embodiment is a container that contains, for example, a mixed solution composed of a sample such as a biological sample and an extract, and in which the mixture is concentrated.
- the sample concentration container 1 includes a container body 2 having an upper surface opening (first opening) 3 and a lid 4 provided on the upper surface opening 3 of the container body 2 so as to be freely opened and closed.
- FIG. 1 is a cross-sectional view showing a main configuration of a specimen concentration container 1 according to an embodiment of the present invention.
- a small cup-shaped (bottomed cylindrical) sample concentrating unit 6 is configured. The sample concentrating unit 6 accommodates the mixed liquid after the concentration process.
- the container body 2 is a bowl-shaped member configured such that the inner peripheral area (area of the cut surface) gradually decreases in order from the upper surface toward the bottom surface. More specifically, as shown in FIG. 1, the shape inside the container body 2 extends with a constant inner peripheral area (corresponding to the inner peripheral area of the upper surface opening 3) in a predetermined section from the upper surface to the bottom surface side. A straight shape portion is formed. Then, after the predetermined section and the straight-shaped portion are continued, a tapered-shaped portion whose inner peripheral area (area of the cut end) gradually decreases is formed. This tapered portion is formed such that the inner peripheral area (cut area) decreases at a constant rate, and the ratio at which the inner peripheral area (cut area) decreases along the way increases further.
- a specimen concentrating portion 6 is formed below the tapered portion and on the bottom side of the container body 2.
- the sample concentrating portion 6 has a cup shape (bottomed cylindrical shape) extended with a constant inner peripheral area (inner peripheral area of the sample concentrating portion 6).
- a spherical sample lid 8 having a diameter larger than the diameter of the upper surface opening 7 of the sample concentrating unit 6 is movable on the upper surface opening 7 of the sample concentrating unit 6 and in the container body 2. Is arranged. Further, the bottom surface and the side surface of the sample concentration unit 6 are covered with a resin or the like in order to prevent the concentrated sample from leaking out at all.
- the specimen lid 8 is made of a material having a specific gravity lighter than water, such as synthetic resin, and is made of PP (polypropylene) resin, PE (polyethylene) resin, or the like. That is, the sample lid 8 only needs to be made of a substance that floats on the mixture 9 of the extract and the sample.
- the sample concentrating unit 6 When the liquid mixture 9 evaporates and its liquid level decreases to the position of the upper surface opening 7 of the sample concentrating unit 6, the sample concentrating unit 6 has a diameter larger than the diameter of the upper surface opening 7 of the sample concentrating unit 6.
- the lid is covered with a spherical specimen lid 8 having a large diameter and a diameter smaller than the inner diameter (diameter of the cut end) of the upper surface opening 3 of the container body 2.
- the container body 2 is provided with a lid 4 so that the upper surface opening 3 can be freely opened and closed. Then, the container body 2 can be sealed by closing the lid 4.
- the specimen lid 8 can be prevented from jumping out of the specimen concentration container 1 during transportation. Even after the injection of the extract or the mixture of the extract and the specimen, the upper surface opening 3 of the container body 2 is closed by the lid 4 so that the extract or the mixture overflows from the upper surface opening 3. Can be prevented.
- a mixed solution 9 of an extract and a sample is stored in the sample concentration container 1.
- the spherical sample lid 8 has a specific gravity lighter than that of the mixed solution 9, and thus is in a state of floating on the liquid surface of the mixed solution 9. Yes.
- FIG. 2 and 3 are cross-sectional views showing an example of a specimen concentration method using the specimen concentration container 1 shown in FIG.
- FIG. 2 shows a state in which the specimen concentration container 1 is disposed on the centrifugal rotating device 10.
- FIG. 3 shows a state in which the sample concentration container 1 arranged in the centrifugal rotator 10 is rotated.
- FIG. 4 is a cross-sectional view showing an example of a specimen concentration method using the specimen concentration container 1 shown in FIG.
- FIG. 4 schematically shows the positional relationship between the liquid surface position of the partially mixed liquid 9 and the sample concentrating unit 6 and the sample lid 8.
- FIG. 5 is a cross-sectional view showing an example of a specimen concentration method using the specimen concentration container 1 shown in FIG.
- FIG. 5 schematically shows the positional relationship between the liquid surface position of the partially mixed liquid 9 and the sample concentrating unit 6 and the sample lid 8.
- a spherical sample lid 8 having a diameter larger than the diameter of the upper surface opening 7 of the sample concentrating unit 6 is disposed on the upper surface opening 7 of the sample concentrating unit 6 in the container body 2 of the sample concentrating container 1.
- the sample lid 8 is covered with the sample lid 8. It becomes.
- the mixed liquid 9 in the sample concentrating unit 6 is maintained. Hardly evaporates.
- the mixed liquid 9 hardly decreases by evaporation. For this reason, the operator does not need to constantly or repeatedly monitor the amount of the liquid mixture in the container body, and can obtain a certain amount of the liquid mixture 9 that is concentrated. Become.
- the container main body 2 of the sample concentration container 1 has a configuration in which the bottomed cylindrical sample concentration unit 6 is formed following the tapered portion as described above.
- the tapered portion in the vicinity of the upper surface opening 7 has an arc shape so as to follow the outer shape of the sample lid 8 as shown in FIG. The shape may be recessed.
- FIG. 6 is a cross-sectional view of the specimen concentration container 1 according to a modification of the embodiment of the present invention.
- FIG. 6 shows a cross-sectional shape of the main body container 2 in the vicinity of the upper surface opening 7 of the specimen concentration unit 6.
- the shape of a predetermined portion of the container body 2 (particularly, the vicinity of the upper surface opening portion 7 of the sample concentrating portion 6). It is advantageous to adopt a configuration that matches the outer shape of the specimen lid 8.
- the predetermined part of the container main body 1 conform to the outer shape of the specimen lid 8
- the degree of sealing in the specimen concentrating part 6 can be further increased, so that the evaporation amount of the mixed liquid is further reduced. be able to.
- the liquid level of the mixed solution 9 is lower than the upper surface opening 7 of the sample concentrating unit 6, the larger the contact area between the sample lid 8 and the container body 2, the larger the evaporation amount of the mixed solution 9. The reduction effect becomes higher.
- the shape of the sample lid 8 according to the present embodiment is not limited to a simple spherical shape.
- the sample lid 8 may include a hollow portion.
- a configuration in which the inside of the specimen lid 8 is hollow may be employed.
- a configuration in which a plurality of small spherical hollow portions (for example, bubbles) are formed inside the specimen lid 8 may be adopted.
- 7 and 8 are sectional views showing a schematic configuration of the specimen lid 8 according to a modification of the present embodiment.
- the specific gravity of the entire sample lid 8 can be lowered. Thereby, even if the liquid mixture 9 is a liquid having a specific gravity smaller than that of water, the sample lid 8 can be caused to function as a floating lid that floats on the liquid surface of the liquid mixture 9.
- the specimen lid 8 may be configured to include the protruding portion 11.
- 9 and 10 are cross-sectional views showing a schematic configuration of the specimen lid 8 according to a modification of the present embodiment.
- the protrusion 11 provided in the sample lid 8 shown in FIGS. 9 and 10 is a rod-shaped member extending in one direction from the sample lid main body 12 formed as a spherical portion of the sample lid 8.
- the combined length of the protruding portion 11 and the spherical portion of the sample lid main body 12, that is, the total length of the sample lid 8 including the protruding portion 11 is one in the container main body 2. It is defined to be longer than the portion where the inner diameter dimension becomes larger.
- a predetermined surface of the sample lid 8 can always be arranged on the upper surface opening 7 side of the sample concentration unit 6.
- the specimen lid 8 shown in FIG. 9 can be arranged so that the spherical surface of the specimen lid main body 12 faces downward and the protruding portion 11 side faces upward.
- the sample lid 8 can seal the upper surface opening 7 of the sample concentrating unit 6 with a predetermined spherical portion at a predetermined timing.
- the inside of the specimen lid 8 may be configured such that the entire interior is hollow as shown in FIG.
- the sample lid 8 includes a protruding portion 11 shorter than the sample lid 8 shown in FIG. 9, and a column-shaped hollow from the tip of the protruding portion 11 to a substantially central position of the sample lid main body 12. It is good also as a structure containing a part. By making the protrusion 11 side hollow in this way, the entire center of gravity of the specimen lid 8 can be biased toward the specimen lid main body 8 side.
- the sample lid 8 is surely directed so that the spherical surface side of the sample lid body 12 faces downward and the protruding portion 11 side is Arranged to face up.
- the sample lid 8 can seal the upper surface opening 7 of the sample concentrating unit 6 with a predetermined spherical portion at a predetermined timing.
- the specimen lid 8 described above is made of PP (polypropylene) resin or PE (polyethylene) resin, but is not limited to these substances.
- the specimen lid 8 may be configured using a resin having elasticity such as a synthetic material with rubber.
- the degree of adhesion with the container body 2 can be increased in the vicinity of the upper surface opening 7 of the specimen concentrating part 6. Therefore, since the evaporation of the mixed solution 9 in the sample concentrating unit 6 can be more effectively prevented, for example, even when the highly volatile mixed solution 9 is used, a certain amount of the mixed solution is used for the sample. It can be left in the concentration section.
- the present invention includes a container body having an open top surface and a lid that is openable and closable at the top surface opening of the container body.
- the container body has an open top surface at an inner bottom surface thereof.
- a cup-shaped specimen concentrating portion whose inner diameter is smaller than the inner diameter of the container body is provided, and the upper surface opening of the specimen concentrating portion has a diameter larger than the diameter of the upper surface opening of the specimen concentrating portion.
- a spherical specimen lid is movably arranged, and is effective, for example, in a concentration process performed using a specimen concentration container such as a Kuderna-Danish concentration tube.
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Abstract
Description
図1に示すごとく、本実施形態の検体濃縮容器1は、例えば、生体試料などの検体と抽出液とからなる混合液を収容し、その内部でこの混合液の濃縮が行われる容器である。検体濃縮容器1は、上面開口部(第1開口部)3を有した容器本体2と、この容器本体2の上面開口部3に開閉自在に設けた蓋4とを備えている。図1は本発明の実施形態に係る検体濃縮容器1の要部構成を示す断面図である。
なお、本実施の形態に係る検体濃縮容器1の容器本体2は、上述したようにテーパー形状部分に続いて、有底筒状の検体濃縮部6が形成される構成であった。しかしながら、検体蓋8による検体濃縮部6の上面開口部7の密閉度を高めるために、上面開口部7近傍のテーパー形状部分が、図6に示すように検体蓋8外形に沿うように弧形状に凹んだ形状となっていてもよい。図6は、本発明の実施形態の変形例に係る検体濃縮容器1の断面図である。図6では、検体濃縮部6の上面開口部7近傍における本体容器2の断面形状を示している。
2 容器本体
3 上面開口部
4 蓋
5 内部底面部
6 検体濃縮部
7 上面開口部
8 検体蓋
9 混合液
10 遠心回転装置
11 突出部
12 検体蓋本体
Claims (15)
- 検体を含む混合液を収容し、その内部で該混合液の濃縮が行われる検体濃縮容器であって、
上面に第1開口部を有する管形状の容器本体と、
前記容器本体内と連通するための第2開口部を有し、該容器本体の底部側に形成された、濃縮後の前記混合液を収容する検体濃縮部と、
前記容器本体内に配置された前記第2開口部を覆うための検体蓋と、を備える検体濃縮容器。 - 前記第2開口部の内径は、前記容器本体の内径よりも小さくなっており、
前記検体濃縮部は、上部に前記第2開口部が形成された有底筒状である請求項1に記載の検体濃縮容器。 - 前記検体蓋は、前記混合液の濃縮時において、該混合液の液面が前記検体濃縮部の前記第2開口よりも低下したとき、該第2開口部を覆う請求項1または2に記載の検体濃縮容器。
- 前記検体蓋は、前記第2開口部の直径よりも大きな直径を有する球状である請求項1~3のいずれか一つに記載の検体濃縮容器。
- 上面が開口した容器本体と、この容器本体の上面開口部に開閉自在に設けた蓋と、を備え、前記容器本体は、その内部底面部に、上面が開口し、その内径が、前記容器本体の内径よりも小さなコップ状の検体濃縮部を設けるとともに、この検体濃縮部の上面開口部上に、この検体濃縮部の上面開口部の直径よりも大きな直径を有する球状の検体蓋を可動自在に配置した検体濃縮容器。
- 前記検体蓋が、合成樹脂により構成される請求項1~5のいずれか一つに記載の検体濃縮容器。
- 前記検体蓋が、ポリプロピレン樹脂により構成される請求項1~6のいずれか一つに記載の検体濃縮容器。
- 前記検体蓋が、ポリエチレン樹脂により構成される請求項1~7のいずれか一つに記載の検体濃縮容器。
- 前記検体蓋は、前記混合液において浮くように構成された請求項1~7のいずれか一つに記載の検体濃縮容器。
- 前記検体蓋は、前記混合液の濃縮時において、該混合液の液面が前記検体濃縮部の前記第2開口よりも低下したとき、該第2開口部近傍で前記容器本体と面接触して、この第2開口部を覆う請求項3に記載の検体濃縮容器。
- 前記検体蓋は、その内部に中空部分を含む請求項9または10に記載の検体濃縮容器。
- 前記検体蓋は、一方向に突出した柱状の突出部を備える請求項9または10に記載の検体濃縮容器。
- 前記突出部を含めた検体蓋の全長が、容器本体の内径よりも長くなる請求項12に記載の検体濃縮容器。
- 前記検体蓋は、前記第2開口部を塞ぐ面を含むとともに、前記突出部が形成されている検体蓋本体を有し、
少なくとも前記突出部の内部が中空となる請求項12に記載の検体濃縮容器。 - 請求項1~14のいずれか一つに記載の検体濃縮容器を用いた検体濃縮方法であって、前記検体濃縮容器内に、抽出液と検体との混合液を配置し、減圧下において、前記検体濃縮容器を遠心機により回転させ、遠心力を発生させて混合液の濃縮を行う検体濃縮方法。
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JP2013553113A JP6026432B2 (ja) | 2012-01-12 | 2012-12-27 | 検体濃縮容器とそれを用いた検体濃縮方法 |
CN201280066857.2A CN104039407B (zh) | 2012-01-12 | 2012-12-27 | 样品浓缩容器和使用它的样品浓缩方法 |
EP12865157.7A EP2803396B1 (en) | 2012-01-12 | 2012-12-27 | Sample-concentrating container and sample-concentrating method using same |
US14/371,714 US9700885B2 (en) | 2012-01-12 | 2012-12-27 | Specimen concentration container and specimen concentrating method using same |
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EP3442705A1 (en) | 2016-04-15 | 2019-02-20 | The Moorhead Group | Well plate |
CN110057899B (zh) * | 2019-04-14 | 2022-01-28 | 北京工业大学 | 厌氧氨氧化污泥胞外聚合物分子结构表征的系统及方法 |
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JPWO2013105204A1 (ja) | 2015-05-11 |
US9700885B2 (en) | 2017-07-11 |
CN104039407B (zh) | 2016-07-06 |
EP2803396A4 (en) | 2015-06-17 |
CN104039407A (zh) | 2014-09-10 |
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