WO2013090360A3 - High resolution dual-objective microscopy - Google Patents

High resolution dual-objective microscopy Download PDF

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Publication number
WO2013090360A3
WO2013090360A3 PCT/US2012/069138 US2012069138W WO2013090360A3 WO 2013090360 A3 WO2013090360 A3 WO 2013090360A3 US 2012069138 W US2012069138 W US 2012069138W WO 2013090360 A3 WO2013090360 A3 WO 2013090360A3
Authority
WO
WIPO (PCT)
Prior art keywords
microscopy
objectives
images
high resolution
entity
Prior art date
Application number
PCT/US2012/069138
Other languages
French (fr)
Other versions
WO2013090360A2 (en
Inventor
Xiaowei Zhuang
Ke Xu
Hazen P. BABCOK
Original Assignee
President And Fellows Of Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by President And Fellows Of Harvard College filed Critical President And Fellows Of Harvard College
Publication of WO2013090360A2 publication Critical patent/WO2013090360A2/en
Publication of WO2013090360A3 publication Critical patent/WO2013090360A3/en

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Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/58—Optics for apodization or superresolution; Optical synthetic aperture systems

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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

The present invention generally relates to super-resolution microscopy. For example, certain aspects of the invention are generally directed to a microscopy system comprising at least two objectives. In some embodiments, the microscopy system may also contain a non-circularly- symmetric lens. One or more images can be obtained using the objectives, for example, using stochastic imaging techniques such as STORM (stochastic optical reconstruction microscopy), optionally in conjunction with entities that are photoactivatable and/or photo switchable. The images obtained using the objectives may be compared, e.g., to remove noise, and/or to compare an entity present in both images, for instance, to determine the z-position of the entity. In some cases, surprisingly high resolutions may be obtained using such techniques, for example, resolutions of better than about 10 nm.
PCT/US2012/069138 2011-12-15 2012-12-12 High resolution dual-objective microscopy WO2013090360A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161576089P 2011-12-15 2011-12-15
US61/576,089 2011-12-15

Publications (2)

Publication Number Publication Date
WO2013090360A2 WO2013090360A2 (en) 2013-06-20
WO2013090360A3 true WO2013090360A3 (en) 2013-08-15

Family

ID=47505331

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/069138 WO2013090360A2 (en) 2011-12-15 2012-12-12 High resolution dual-objective microscopy

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WO (1) WO2013090360A2 (en)

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Publication number Priority date Publication date Assignee Title
US9063434B2 (en) 2013-01-11 2015-06-23 University Of Utah Research Foundation Sub-diffraction-limited patterning and imaging via multi-step photoswitching
ES2461015B2 (en) * 2013-10-10 2015-05-22 Universidad De Cantabria Microscope for spectroscopic characterization of a sample
CA2938082A1 (en) 2013-12-15 2015-06-18 President And Fellows Of Harvard College Methods and compositions relating to optical super-resolution patterning
CN107655812A (en) * 2013-12-18 2018-02-02 香港科技大学 Method, system and the prismatic light chip device of deep layer cells super-resolution imaging
JP6355334B2 (en) * 2013-12-27 2018-07-11 株式会社キーエンス Magnification observation apparatus, magnification image observation method, magnification image observation program, and computer-readable recording medium
EP3174993B1 (en) 2014-07-30 2023-12-06 President and Fellows of Harvard College Probe library construction
US20160069903A1 (en) 2014-09-10 2016-03-10 Fundació Institute De Ciències Foròniques Method for detecting cells
US10006917B2 (en) 2014-12-15 2018-06-26 President And Fellows Of Harvard College Methods and compositions relating to super-resolution imaging and modification
CN105510290A (en) * 2015-12-22 2016-04-20 浙江大学 Non-linear super-resolution microscopic method and device adopting photon recombination
US10783697B2 (en) 2016-02-26 2020-09-22 Yale University Systems, methods, and computer-readable media for ultra-high resolution 3D imaging of whole cells
LU93022B1 (en) * 2016-04-08 2017-11-08 Leica Microsystems Method and microscope for examining a sample
EP3539036A4 (en) 2016-11-08 2020-06-17 President and Fellows of Harvard College Matrix imprinting and clearing
US20190276881A1 (en) 2016-11-08 2019-09-12 President And Fellows Of Harvard College Multiplexed imaging using merfish, expansion microscopy, and related technologies
US11946867B2 (en) 2016-12-05 2024-04-02 Memorial Sloan Kettering Cancer Center Modulation interferometric imaging systems and methods
US11788123B2 (en) 2017-05-26 2023-10-17 President And Fellows Of Harvard College Systems and methods for high-throughput image-based screening
EP3418391B1 (en) 2017-06-23 2022-09-28 Fundació Institut de Ciències Fotòniques Method for quantifying protein copy-number
US20240060121A1 (en) 2019-11-20 2024-02-22 President And Fellows Of Harvard College Methods for multi-focal imaging for molecular profiling
WO2021108493A1 (en) * 2019-11-27 2021-06-03 Temple University-Of The Commonwealth System Of Higher Education Method and system for enhanced photon microscopy
WO2022032194A1 (en) * 2020-08-06 2022-02-10 Singular Genomics Systems, Inc. Methods for in situ transcriptomics and proteomics
CN112986286B (en) * 2021-02-19 2022-09-30 天津大学 X-ray double-view-field microscopic imaging detection system and imaging method thereof
WO2024013261A1 (en) * 2022-07-12 2024-01-18 Oxford Nanoimaging, Inc. Quantitative measurement of molecules using single molecule fluorescence microscopy

Citations (2)

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WO2009085218A1 (en) * 2007-12-21 2009-07-09 President And Fellows Of Harvard College Sub-diffraction limit image resolution in three dimensions
US20100231922A1 (en) * 2006-12-21 2010-09-16 Howard Hughes Medical Institute Three-dimensional interferometric microscopy

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7838302B2 (en) 2006-08-07 2010-11-23 President And Fellows Of Harvard College Sub-diffraction limit image resolution and other imaging techniques
US7776613B2 (en) 2006-08-07 2010-08-17 President And Fellows Of Harvard College Sub-diffraction image resolution and other imaging techniques

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100231922A1 (en) * 2006-12-21 2010-09-16 Howard Hughes Medical Institute Three-dimensional interferometric microscopy
WO2009085218A1 (en) * 2007-12-21 2009-07-09 President And Fellows Of Harvard College Sub-diffraction limit image resolution in three dimensions

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
G. SHTENGEL ET AL: "Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 106, no. 9, 3 March 2009 (2009-03-03), pages 3125 - 3130, XP055007791, ISSN: 0027-8424, DOI: 10.1073/pnas.0813131106 *

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