WO2013075263A1 - 热对流聚合酶连锁反应装置 - Google Patents
热对流聚合酶连锁反应装置 Download PDFInfo
- Publication number
- WO2013075263A1 WO2013075263A1 PCT/CN2011/001941 CN2011001941W WO2013075263A1 WO 2013075263 A1 WO2013075263 A1 WO 2013075263A1 CN 2011001941 W CN2011001941 W CN 2011001941W WO 2013075263 A1 WO2013075263 A1 WO 2013075263A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- test tube
- polymerase chain
- chain reaction
- thermal convection
- heat sink
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
- B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
- B01L2300/0838—Capillaries
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1861—Means for temperature control using radiation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0442—Moving fluids with specific forces or mechanical means specific forces thermal energy, e.g. vaporisation, bubble jet
- B01L2400/0445—Natural or forced convection
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/06—Test-tube stands; Test-tube holders
- B01L9/065—Test-tube stands; Test-tube holders specially adapted for capillary tubes
Definitions
- the invention relates to a polymerase chain reaction, and in particular to a thermal convection polymerase chain reaction device. current technology
- PCR polymerase chain reaction
- the temperature of the denaturation reaction is typically between 90 and 97 ° C; the temperature of the primer bonding reaction is selected depending on the melting temperature of the primer used, typically between 35 and 65 ° C; typical of the extension reaction The temperature is 72 °C.
- the thermal convection polymerase chain reaction method mainly embeds the bottom of the test tube containing the PCR mixture in hot water, and the rest of the test tube is exposed to room temperature air to dissipate heat, so that the bottom of the PCR mixture is gradually reduced to the surface.
- the temperature gradient is 9, for example. C to 35 °C to induce thermal convection, allowing the PCR mixture to undergo different reaction steps through different temperature zones.
- a primary object of the present invention is to provide a thermal convection polymerase chain reaction apparatus which ensures that the temperature of the surface of the mixed solution during the PCR reaction is lower than the temperature required for the bonding reaction.
- the present invention provides a thermal convection polymerase chain reaction device for holding a test tube to carry out a polymerase chain reaction in the test tube, characterized in that the device comprises: a heat dissipation
- the seat has a body, and a passage is disposed through the body for receiving the test tube; wherein the passage has a large diameter section and a small diameter section located below the large diameter section.
- a test tube rack is disposed above the heat sink, and the test tube rack has a receiving hole for inserting the test tube.
- the receiving hole of the test tube rack has a large diameter portion, a shoulder portion and a small diameter portion from top to bottom, wherein the shoulder portion abuts against the first step portion of the test tube.
- the bottom end of the test tube rack is inserted into the channel of the heat sink.
- the inner diameter of the small diameter section of the passage is gradually reduced from the top to the bottom.
- the heat sink is made of metal.
- the present invention ensures that the temperature of the surface of the mixed solution during the PCR reaction is lower than the temperature required for the adhesion reaction of the primer.
- the invention is applicable to the thermal convection polymerase chain reaction in the field of biotechnology.
- Figure 1 is a schematic view of a preferred embodiment of the present invention
- Figure 2 is a perspective exploded view of a preferred embodiment of the present invention.
- the thermal convection polymerase chain reaction apparatus 10 mainly comprises a heat sink 20 and a test tube rack 30.
- the heat sink 20 has a body 22, and a channel 24 is disposed through the body 22 for receiving the test tube 12, wherein the passage 24 has a large diameter section 241 and a small diameter section 242 located below the large diameter section 241.
- the heat sink 20 Since the middle section 123 to the upper section 122 of the test tube 12 are disposed in the passage 24 of the heat sink 20, and the heat sink 20 is made of a material having a high thermal conductivity such as an aluminum alloy, the heat energy of the PCR mixture can be conducted to the surrounding air to The heat sink 20 is more efficiently dissipated, so that the temperature of the surface of the mixed liquid is maintained below the primer bonding temperature during the whole reaction process, for example, 10 to 55 ° C, to avoid the PCR reaction due to the excessive surface temperature of the mixed liquid. Interrupted. Furthermore, the mixed solution located in the large diameter section 241 dissipates heat slowly, and the mixed solution located in the small diameter section 242 dissipates heat rapidly. After experimental tests, it is found that the heat dissipation effect of the structure of the heat sink 20 is most favorable for the PCR reaction.
- a test tube rack 30 is disposed above the heat sink 20, and the test tube rack 30 has a receiving hole 32 for inserting the test tube 12, so that the test tube 12 can be stably positioned.
- the outer wall of the test tube 12 is prevented from coming into contact with the inner edge of the heat sink 20, so that the temperature of the mixture is lowered too quickly.
- the receiving hole 32 of the test tube rack 30 can be designed to be provided with a large diameter portion 321 , a shoulder portion 322 and a small diameter portion 323 from top to bottom, wherein the shoulder portion 322 is used for one of the test tubes 12 .
- the step 121 abuts, whereby the test tube 12 can be stably positioned.
- a support base 40 is disposed under the heat sink 20, and the test tube bottom 124 is heated by a heat source (not shown) to maintain the temperature of the PCR mixture in the bottom portion 124 at 90 to 97 °C.
- the bottom end 301 of the test tube rack 30 is inserted into the passage 24 of the heat sink 20, so that the test tube rack 30 is firmly disposed above the heat sink 20.
- the inner diameter of the small diameter section 242 of the passage 24 is gradually reduced from the top to the bottom.
- the structure of the device may also be varied without departing from the spirit of the invention.
- the heat sink 20 may be provided with a plurality of channels 24 for simultaneous reaction of a plurality of test tubes 12 (shown in Figure 2). All such structural changes that are easily conceivable are covered by the scope of patent protection of the present invention.
- the thermal convection polymerase chain reaction device provided by the invention is suitable for the thermal convection polymerization enzyme chain reaction in the biotechnology field, and ensures that the temperature of the surface of the mixed solution is lower than the temperature required for the adhesion reaction of the primer during the PCR reaction.
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
Claims
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020147002887A KR101691466B1 (ko) | 2011-11-22 | 2011-11-22 | 열대류 중합효소 연쇄반응 장치 |
CN201180072273.1A CN103649301B (zh) | 2011-11-22 | 2011-11-22 | 热对流聚合酶连锁反应装置 |
CA2856345A CA2856345C (en) | 2011-11-22 | 2011-11-22 | Device for thermal convection polymerase chain reaction |
IN3868CHN2014 IN2014CN03868A (zh) | 2011-11-22 | 2011-11-22 | |
PCT/CN2011/001941 WO2013075263A1 (zh) | 2011-11-22 | 2011-11-22 | 热对流聚合酶连锁反应装置 |
EP11876152.7A EP2784150B1 (en) | 2011-11-22 | 2011-11-22 | Device for polymerase chain reaction via thermal convection |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/CN2011/001941 WO2013075263A1 (zh) | 2011-11-22 | 2011-11-22 | 热对流聚合酶连锁反应装置 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2013075263A1 true WO2013075263A1 (zh) | 2013-05-30 |
Family
ID=48468973
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/CN2011/001941 WO2013075263A1 (zh) | 2011-11-22 | 2011-11-22 | 热对流聚合酶连锁反应装置 |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP2784150B1 (zh) |
KR (1) | KR101691466B1 (zh) |
CN (1) | CN103649301B (zh) |
CA (1) | CA2856345C (zh) |
IN (1) | IN2014CN03868A (zh) |
WO (1) | WO2013075263A1 (zh) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108949545A (zh) * | 2018-08-16 | 2018-12-07 | 上海海洋大学 | 一种新型核酸等温扩增反应组件 |
CN109554295A (zh) * | 2019-01-21 | 2019-04-02 | 武汉理工大学 | 远洋船员的pcr扩增与疾病检测装置 |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR102389800B1 (ko) * | 2015-05-12 | 2022-04-22 | 시아먼 유니버시티 | 액체 순환 경로를 조절할 수 있는 핵산 증폭 반응 튜브 |
CN105670924A (zh) * | 2016-03-01 | 2016-06-15 | 上海理工大学 | 自然对流聚合酶链反应微系统 |
CN107964507B (zh) | 2016-10-18 | 2022-09-27 | 财团法人工业技术研究院 | 热对流聚合酶连锁反应装置及其光学检测方法 |
CN108410688A (zh) * | 2017-02-09 | 2018-08-17 | 克雷多生物医学私人有限公司 | 一种热对流式聚合酶链式反应的装置 |
CN109401948A (zh) * | 2018-11-01 | 2019-03-01 | 福建省博凯科技有限公司 | 一段式热对流pcr仪 |
CN109294901A (zh) * | 2018-11-01 | 2019-02-01 | 福建省博凯科技有限公司 | 一段式热对流pcr仪及控制方法 |
Citations (2)
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CN101735944A (zh) * | 2008-11-18 | 2010-06-16 | 霍夫曼-拉罗奇有限公司 | 毛细管 |
CN101855017A (zh) * | 2007-08-03 | 2010-10-06 | 恩尼格马诊断有限公司 | 包括传导层和内部非金属层的反应容器 |
Family Cites Families (5)
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CA2130013C (en) | 1993-09-10 | 1999-03-30 | Rolf Moser | Apparatus for automatic performance of temperature cycles |
KR100488281B1 (ko) * | 2001-09-15 | 2005-05-10 | 아람 바이오시스템 주식회사 | 열 대류를 이용한 염기서열 증폭 방법 및 장치 |
DE102004039092B4 (de) | 2004-08-11 | 2008-04-24 | Deutsches Zentrum für Luft- und Raumfahrt e.V. | Verriegelungsvorrichtung für Linearaktuatoren |
US8153064B2 (en) * | 2007-03-22 | 2012-04-10 | Doebler Ii Robert W | Systems and devices for isothermal biochemical reactions and/or analysis |
JP5865261B2 (ja) * | 2010-01-12 | 2016-02-17 | アーラム バイオシステムズ インコーポレイテッド | 2段熱対流装置及びその使用法 |
-
2011
- 2011-11-22 WO PCT/CN2011/001941 patent/WO2013075263A1/zh active Application Filing
- 2011-11-22 EP EP11876152.7A patent/EP2784150B1/en active Active
- 2011-11-22 KR KR1020147002887A patent/KR101691466B1/ko active IP Right Grant
- 2011-11-22 CA CA2856345A patent/CA2856345C/en active Active
- 2011-11-22 IN IN3868CHN2014 patent/IN2014CN03868A/en unknown
- 2011-11-22 CN CN201180072273.1A patent/CN103649301B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101855017A (zh) * | 2007-08-03 | 2010-10-06 | 恩尼格马诊断有限公司 | 包括传导层和内部非金属层的反应容器 |
CN101735944A (zh) * | 2008-11-18 | 2010-06-16 | 霍夫曼-拉罗奇有限公司 | 毛细管 |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108949545A (zh) * | 2018-08-16 | 2018-12-07 | 上海海洋大学 | 一种新型核酸等温扩增反应组件 |
CN109554295A (zh) * | 2019-01-21 | 2019-04-02 | 武汉理工大学 | 远洋船员的pcr扩增与疾病检测装置 |
Also Published As
Publication number | Publication date |
---|---|
KR101691466B1 (ko) | 2016-12-30 |
CN103649301B (zh) | 2015-03-11 |
CA2856345A1 (en) | 2013-05-30 |
IN2014CN03868A (zh) | 2015-10-16 |
CA2856345C (en) | 2017-10-24 |
KR20140040262A (ko) | 2014-04-02 |
EP2784150B1 (en) | 2019-05-15 |
CN103649301A (zh) | 2014-03-19 |
EP2784150A1 (en) | 2014-10-01 |
EP2784150A4 (en) | 2015-08-12 |
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