WO2013072495A3 - Verfahren, photobioreaktor und photosyntheseschichten zur kultur photoautotropher mikroorganismen - Google Patents

Verfahren, photobioreaktor und photosyntheseschichten zur kultur photoautotropher mikroorganismen Download PDF

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Publication number
WO2013072495A3
WO2013072495A3 PCT/EP2012/072909 EP2012072909W WO2013072495A3 WO 2013072495 A3 WO2013072495 A3 WO 2013072495A3 EP 2012072909 W EP2012072909 W EP 2012072909W WO 2013072495 A3 WO2013072495 A3 WO 2013072495A3
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Prior art keywords
photosynthesis
micro
organisms
accommodation space
medium
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PCT/EP2012/072909
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English (en)
French (fr)
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WO2013072495A2 (de
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Rudolf Ehwald
Lars BÄHR
Arne WÜSTENBERG
Joel Herve SOH
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Humboldt-Universität Zu Berlin
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Priority to US14/358,045 priority Critical patent/US9200244B2/en
Publication of WO2013072495A2 publication Critical patent/WO2013072495A2/de
Publication of WO2013072495A3 publication Critical patent/WO2013072495A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/06—Tubular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/26—Conditioning fluids entering or exiting the reaction vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/08—Means for providing, directing, scattering or concentrating light by conducting or reflecting elements located inside the reactor or in its structure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Es wird ein Verfahren zur Kultivierung von photoautotrophen Mikroorganismen vorgeschlagen, umfassend: - Bereitstellen einer ein Photosynthesemedium und darin suspendierte photoautotrophe Mikroorganismen enthaltenden Photosyntheseschicht (2) in einem abgeschlossenen Aufnahmeraum mit einer gasdiffusionshemmenden Außenbegrenzung (1), wobei die Photosyntheseschicht (2) eine mittlere Dicke von nicht mehr als 10 mm aufweist; - Belichten der Photosyntheseschicht (2) ohne das Verwirbeln oder Durchleiten eines Gases im Photosynthesemedium unter Begrenzung des diffusiven Gasaustausches des Aufnahmeraumes mit der Umgebung, wobei die Versorgung der Mikroorganismen im Photosynthesemedium mit Hydrogencarbonationen durch das Speichern von Hydrogencarbonationen im Photosynthesemedium und/oder das Liefern eines Photosynthesesubstrats in Form von Kohlendioxid und/oder Hydrogencarbonat aus einem zellfreien Kohlenstoffvorratsmedium (4) außerhalb der Photosyntheseschicht aufrechterhalten wird; und - Ableiten eines bei der Photosynthese gebildeten Gases aus dem Aufnahmeraum. Weiterhin wird ein Photobioreaktor zur Kultivierung von photoautotrophen Mikroorganismen vorgeschlagen, der einen abgeschlossenen Aufnahmeraum mit einer Gasdiffusion-hemmenden Außenbegrenzung umfasst, wobei der Aufnahmeraum aufweist: - ein erstes Kompartiment zur Ausbildung einer Photosyntheseschicht (2), die eine mittlere Dicke von nicht mehr als 10 mm aufweist; - und mindestens ein zweites Kompartiment zur Aufnahme eines Kohlenstoffvorratsmediums (4), das vom ersten Kompartiment durch mindestens eine Membran getrennt ist, die durchlässig ist für mindestens ein Photosynthesesubstrat ausgewählt aus der Gruppe umfassend Kohlendioxid und Hydrogencarbonationen.
PCT/EP2012/072909 2011-11-17 2012-11-16 Verfahren, photobioreaktor und photosyntheseschichten zur kultur photoautotropher mikroorganismen WO2013072495A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US14/358,045 US9200244B2 (en) 2011-11-17 2012-11-16 Method for culturing photoautotrophic microorganisms for the production of biomass

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102011055448A DE102011055448A1 (de) 2011-11-17 2011-11-17 Verfahren, Photobioreaktor und Photosyntheseschichten zur Kultur photoautotropher Mikroorganismen
DE102011055448.3 2011-11-17

Publications (2)

Publication Number Publication Date
WO2013072495A2 WO2013072495A2 (de) 2013-05-23
WO2013072495A3 true WO2013072495A3 (de) 2013-08-22

Family

ID=47351580

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2012/072909 WO2013072495A2 (de) 2011-11-17 2012-11-16 Verfahren, photobioreaktor und photosyntheseschichten zur kultur photoautotropher mikroorganismen

Country Status (3)

Country Link
US (1) US9200244B2 (de)
DE (1) DE102011055448A1 (de)
WO (1) WO2013072495A2 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10072239B1 (en) * 2013-03-05 2018-09-11 The United States Of America As Represented By The Administrator Of The Nasa Microorganism cultivation platform for human life support
DE102013015969B4 (de) 2013-09-25 2016-11-10 Celldeg Gbr(Vertretungsberechtigter Gesellschafter: Prof.Dr. Rudolf Ehwald, 10115 Berlin Labor-Photobioreaktor
DE102014000691A1 (de) * 2014-01-17 2015-07-23 Forschungszentrum Jülich GmbH Fachbereich Patente Photobioreaktor und Verfahren zur Dünnschichtkultivierung
DE102014225476B4 (de) 2014-12-10 2019-07-04 Gesellschaft zur Förderung von Medizin-, Bio- und Umwelttechnologien e.V. Verfahren zur Kultivierung von nicht-axenischen phototrophen Mikroorganismen in semi-geschlossenen Reaktorsystemen unter Verwendung von Kohlenstoffdioxid (CO2)-Begasung
GB201521136D0 (en) * 2015-12-01 2016-01-13 Arborea Ltd Device
EP3443994B1 (de) * 2017-08-17 2020-04-08 Gambro Lundia AB Verfahren zur sterilisierung wassergefüllter vorrichtungen
CN108018194A (zh) * 2018-01-12 2018-05-11 华南理工大学 一种用于微藻规模培养的管道式光生物反应器
WO2021129923A1 (de) * 2019-12-23 2021-07-01 Marigan Ag Vorrichtung zur kultivierung von photosynthese betreibenden mikroorganismen

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008055190A2 (en) * 2006-11-02 2008-05-08 Algenol Biofuels Limited Closed photobioreactor system for production of ethanol
EP2258463A1 (de) * 2008-03-14 2010-12-08 Endesa Generación, S.A. Flüssigphasen-gassammlung

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Publication number Priority date Publication date Assignee Title
DE102008029169A1 (de) 2008-06-19 2009-12-24 Ehwald, Rudolf, Prof. Dr. Gas-Reservoir für Versuchs- und Kulturgefäße
WO2010012028A1 (en) * 2008-07-29 2010-02-04 Bioseq Pty Ltd Covered pond-type photobioreactor for large-scale, intensive cultivation of microalgae
EP2316917A1 (de) * 2009-11-03 2011-05-04 HF Biotec Berlin GmbH Verfahren zur mixotrophen Kultivierung von Mikroorganismen und/oder Zellen
DE102010021154A1 (de) * 2010-05-21 2011-11-24 Karlsruher Institut für Technologie Photobioreaktor
IT1402640B1 (it) * 2010-11-04 2013-09-13 Poli De "metodo e relativo impianto per la coltivazione di microrganismi fotosintetici"
US20130319059A1 (en) * 2010-12-09 2013-12-05 Washington State University Integrated carbon capture and algae culture
EP2486790A1 (de) * 2011-02-11 2012-08-15 LGem B.V. Verfahren und Bioreaktor zur Kultivierung von Mikroorganismen

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008055190A2 (en) * 2006-11-02 2008-05-08 Algenol Biofuels Limited Closed photobioreactor system for production of ethanol
EP2258463A1 (de) * 2008-03-14 2010-12-08 Endesa Generación, S.A. Flüssigphasen-gassammlung

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HU Q ET AL: "Ultrahigh-cell-density culture of a marine green alga Chlorococcum littorale in a flat-plate photobioreactor", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 49, no. 6, June 1998 (1998-06-01), pages 655 - 662, XP002693728, ISSN: 0175-7598 *
IPAL GESELLSCHAFT FÜR PATENTVERWERTUNG BERLIN MBH: "Photobioreactor", 14 November 2012 (2012-11-14), XP002693727, Retrieved from the Internet <URL:http://www.ipal.de/sites/default/files/to_11033_jab_04092012.pdf> [retrieved on 20130305] *

Also Published As

Publication number Publication date
US20140315280A1 (en) 2014-10-23
DE102011055448A1 (de) 2013-05-23
US9200244B2 (en) 2015-12-01
WO2013072495A2 (de) 2013-05-23

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