WO2013063666A1 - Use of natamycin as an additive for producing silage - Google Patents
Use of natamycin as an additive for producing silage Download PDFInfo
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- WO2013063666A1 WO2013063666A1 PCT/BR2012/000084 BR2012000084W WO2013063666A1 WO 2013063666 A1 WO2013063666 A1 WO 2013063666A1 BR 2012000084 W BR2012000084 W BR 2012000084W WO 2013063666 A1 WO2013063666 A1 WO 2013063666A1
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- WIPO (PCT)
- Prior art keywords
- natamycin
- silage
- additive
- production
- additives
- Prior art date
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
- A23K30/10—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder
- A23K30/15—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging
Definitions
- the present model refers to the technical field of feed additives for feed preservation, specifically to the use of Natamycin as an additive for silage production, which brings as novelty the application of a natural antimycotic agent produced by Streptomyces natalensis fungi. or Streptomyces gilvosporeus or others.
- the model has the function of reducing fungal contamination and spoilage in corn, sorghum, sugar cane, other grass and legume silages and other plant species used for silage production, during fermentation and after the opening of the silos.
- chemical and / or microbial additives are commonly found during the manufacture of forage plant silages.
- Chemical additives contain chemical substances, usually salts of organic acids, to act on the metabolism of microorganisms present in silage.
- Microbial additives contain strains of homolactic or heterolactic bacteria that produce organic acids during the anaerobic phase of the ensiling process. All models found are part of the same technical field, but are completely distinct from the proposed invention.
- the present model was developed as an alternative to additives commonly used in silages as it is a natural antibiotic with specific action on yeast and unicellular fungi, without exerting negative effect on the growth of desirable fermentative bacteria in silages.
- natamycin in the ensiling process reduces the activity of yeast in the ensiling process, avoiding ethanolic fermentation and ensuring greater stability of the silage after the opening of the silos.
- Natamycin is already used in the human food industry, mainly sausages, but is not yet used in the production of fermented feed (silage).
- the product is already approved and used in the preservation of food for human consumption;
- the cost of production is compatible with the benefits that natamycin has on silage, such as reduced ethanol production and increased aerobic stability of silages; - is water soluble and extremely practical to use;
- - may be applied to any type of silage, regardless of how it is produced and the type of silo;
- natamycin in soluble powder form or in liquid solution or otherwise, as an additive in the forage plant silage production process such as corn, sorghum, sugar cane, grass, sunflower , among others, aiming at the feeding of production animals, mainly ruminants.
- Natamycin may be used as a sole additive or in combination with chemicals and / or microbial cultures to form mixed additives.
- the forage used may have original moisture content, or be partially dehydrated (withered) or added with moisture absorbers.
- the natamycin-containing additive may be applied during silage production, diluted with water, sprayed on chopped forage before or during compaction in the silo, or otherwise applied.
- Natamycin may be mixed with vehicles to facilitate homogenization and spraying such as lactose, sugars, soluble chemical powders and granulates, among others, as well as microbial cultures of lactobacilli or other microorganisms and / or chemicals.
- the dosages applied range from 1 to 16 grams of natamycin per tonne of chopped forage, preferably between 2 and 8 g / ⁇ ( more preferably between 4 and 6 g / t, according to the purpose and potential for food spoilage.
- the other steps of the ensiling process are not altered or influenced by the adoption of the natamycin application technique at the time of ensiling.
- Natamycin is a natural antibiotic produced from culture of Streptomyces natalensis bacteria and / or other Streptomyces species. It has crystalline structure and empirical formula C33H47N013, and acts in association with ergosterol, 24 and 28-dehydroergosterol, and cholesterol, present in the membrane of molds and yeast, but absent in bacteria, making natamycin a potent antibiotic inhibitor. yeast and mold, but without effect on bacteria.
- natamycin as an additive in silage production allows selective exclusion of undesirable microorganisms in the process, such as fungi, molds and yeast, without harming the growth of desirable bacteria, especially acid-lactic bacteria.
- the operation is simple and practical, simply adding the additive containing natamycin in solid powder form, or preferably diluted with water and / or other solvent, during the silage preparation process, where the forage plant is minced, natamycin added and compacted. in a storage structure called silo, which silo may have variable shape and size.
- the application of the natamycin-containing additive in solid or liquid or other form may be done manually or by means of implements developed for this purpose, such as watering cans, spray pumps, or others. Natamycin inhibits growth undesirable microorganisms during the aerobic and anaerobic phases of the fermentation process, as well as after the opening of the silos.
- natamycin at dosages of 1 to 16 grams of natamycin per tonne of chopped forage, preferably between 2 and 8 g / t, more preferably between 4 and 6 g / t, becomes Fully efficient, practical and cost-effective means to improve quality and increase the nutritional value of forage plant silages by inhibiting and reducing undesirable microorganism populations, making inoculated silages more stable when exposed to air. For this reason, this technique should be well accepted by technicians and farmers in general, who use additives in the silage process.
Abstract
The present model, "Natamycin as an additive for producing silage", relates to the technical field of additives for preserving fodder intended for feeding animals, more specifically to the use of natamycin as an additive for ensilage, said model being novel due to the use of a selective antifungal agent during the production of silage. The model is designed to inhibit the development of yeasts, fungi and moulds during the aerobic and anaerobic phases of the fermentation process, and after opening the silo and exposing the silage to air.
Description
USO DA NATAMICINA CÒMO ADITIVO NA PRODUÇÃO DE SILAGENS USE OF ADDITIVE NATAMICINE IN SILAGE PRODUCTION
Autores: Patrick Schmidt; Charles Ortiz Novinski Authors: Patrick Schmidt; Charles Ortiz Novinski
Apresentação Presentation
Refere-se o presente modelo, ao campo técnico de aditivos para conservação de forragens destinadas à alimentação animal, mais especificamente ao uso da Natamicina como aditivo para produção de silagens, que traz como novidade a aplicação de um agente antimicótico natural produzido pelos fungos Streptomyces natalensis ou Streptomyces gilvosporeus ou outros. O modelo possui as funções de reduzir contaminação e espoliação de fungos em silagens de milho, sorgo, cana-de-açúcar, outras gramíneas e leguminosas e demais espécies vegetais usadas para produção de silagens, durante a fermentação e após a abertura dos silos. The present model refers to the technical field of feed additives for feed preservation, specifically to the use of Natamycin as an additive for silage production, which brings as novelty the application of a natural antimycotic agent produced by Streptomyces natalensis fungi. or Streptomyces gilvosporeus or others. The model has the function of reducing fungal contamination and spoilage in corn, sorghum, sugar cane, other grass and legume silages and other plant species used for silage production, during fermentation and after the opening of the silos.
Estado da técnica State of the art
No atual estado da técnica são encontrados aditivos químicos e/ou microbianos normalmente aplicados durante a confecção das silagens de plantas forrageiras. Os aditivos químicos contém substâncias químicas, usualmente sais de ácidos orgânicos, com finalidade de agir sobre o metabolismo de microrganismos presentes na silagem. Os aditivos microbianos contém cepas de bactérias homoláticas ou heteroláticas que produzem ácidos orgânicos durante a fase anaeróbia do processo de ensilagem. Todos os modelos encontrados fazem parte do mesmo campo técnico, porém são completamente distintos da invenção proposta. In the current state of the art, chemical and / or microbial additives are commonly found during the manufacture of forage plant silages. Chemical additives contain chemical substances, usually salts of organic acids, to act on the metabolism of microorganisms present in silage. Microbial additives contain strains of homolactic or heterolactic bacteria that produce organic acids during the anaerobic phase of the ensiling process. All models found are part of the same technical field, but are completely distinct from the proposed invention.
A PI0305600-7 A2 "COMPOSIÇÃO CONTENDO LACTOBACILLUS A PI0305600-7 A2 "COMPOSITION CONTAINING LACTOBACILLUS
BUCHNERI AGINDO COMO ADITIVO NA ENSILAGEM SOBRE FERMENTAÇÃO, ESTABILIDADE DA SILAGEM E DESEMPENHO DE ANIMAIS RECEBENDO RAÇÕES CONTENDO SILAGENS DE CANA-DE-AÇÚCAR" revela um aditivo microbiano contendo cepas de Lactobacillus
buchneri que adicionado à silagem de cana visa melhorar características da fermentação e o desempenho de animais. A PI0514826-0 A2 "COMPOSIÇÕES PARA O USO COMO INÓCULOS DE SILAGEM, MÉTODOS DE TRATAMENTO DE MATERIAIS VEGETAIS PRÉ- ENSILADOS E LINHAGEM BACTERIANA SUBSTANCIALMENTE PURIFICADA" trata de linhagens bacterianas purificadas produtoras de ferulato esterase que podem ser usadas como aditivo de forragem e inoculo de silagem. Ambas, porém, são completamente diferentes da invenção aqui proposta. BUCHNERI ACTING AS AN ADDITIVE IN SOIL ON FERMENTATION, SILAGE STABILITY AND ANIMAL PERFORMANCE RECEIVING FEEDS CONTAINING SUGAR CANE SILAGENS "reveals a microbial additive containing Lactobacillus strains Buchneri which added to sugarcane silage aims to improve fermentation characteristics and animal performance. PI0514826-0 A2 "COMPOSITIONS FOR USE AS SILAGE INOCULES, PRE-SOILED VEGETABLE TREATMENT METHODS AND SUBSTANCIALLY PURIFIED BACTERIAL LINE" deals with purified bacterial strains of ferulate esterase that can be used as an additive for inoculation silage. Both, however, are completely different from the invention proposed herein.
Melhorias apresentadas Improvements Presented
O modelo em apreço foi desenvolvido como alternativa aos aditivos comumente usados em silagens por ser um antibiótico natural com ação específica sobre leveduras e fungos unicelulares, sem exercer efeito negativo sobre o crescimento das bactérias fermentativas desejáveis das silagens. Dessa forma, o uso da natamicina no processo de ensilagem reduz a atividade de leveduras no processo de ensilagem, evitando a fermentação etanólica e assegurando maior estabilidade da silagem após a abertura dos silos. The present model was developed as an alternative to additives commonly used in silages as it is a natural antibiotic with specific action on yeast and unicellular fungi, without exerting negative effect on the growth of desirable fermentative bacteria in silages. Thus, the use of natamycin in the ensiling process reduces the activity of yeast in the ensiling process, avoiding ethanolic fermentation and ensuring greater stability of the silage after the opening of the silos.
A Natamicina já é empregada na indústria de alimentos para humanos, principalmente embutidos, mas ainda não é usada na produção de alimentos fermentados para animais (silagem). Natamycin is already used in the human food industry, mainly sausages, but is not yet used in the production of fermented feed (silage).
Os principais benefícios trazidos por este modelo são: The main benefits brought by this model are:
- inibição seletiva de leveduras e fungos filamentosos pela natamicina; - selective inhibition of yeast and filamentous fungi by natamycin;
- o produto já é aprovado e usado na conservação de alimentos para consumo humano; - the product is already approved and used in the preservation of food for human consumption;
- o custo de produção é compatível aos benefícios que a natamicina apresenta na ensilagem, como redução na produção de etanol e elevação na estabilidade aeróbia das silagens;
- é solúvel em água e extremamente prática de ser utilizado;- the cost of production is compatible with the benefits that natamycin has on silage, such as reduced ethanol production and increased aerobic stability of silages; - is water soluble and extremely practical to use;
- tem ação efetiva nas fases aeróbia e anaeróbia de produção das silagens, inclusive após a abertura dos silos; - has effective action in the aerobic and anaerobic phases of silage production, including after the opening of the silos;
- poderá ser aplicado em qualquer tipo de silagem, independente da forma como é produzida e do tipo de silo; e - may be applied to any type of silage, regardless of how it is produced and the type of silo; and
- não prejudica o desenvolvimento e metabolismo das bactérias homofermentativas e heterofermentativas desejáveis no processo de ensilagem. - does not hinder the development and metabolism of desirable homofermentative and heterofermentative bacteria in the ensiling process.
Revelação do modelo Model Reveal
Trata-se da aplicação da natamicina, na forma em pó solúvel ou em solução líquida ou outra forma, como aditivo no processo de produção de silagens de plantas forrageiras, como o milho, o sorgo, a cana-de-açúcar, capins, girassol, entre outras, visando a alimentação de animais de produção, principalmente ruminantes. A natamicina poderá ser usada como aditivo exclusivo, ou combinada com substâncias químicas e/ou culturas microbianas, formando aditivos mistos. A forragem usada pode apresentar teor de umidade original, ou ser parcialmente desidratada (emurchecida) ou acrescida de absorventes de umidade. The application of natamycin, in soluble powder form or in liquid solution or otherwise, as an additive in the forage plant silage production process such as corn, sorghum, sugar cane, grass, sunflower , among others, aiming at the feeding of production animals, mainly ruminants. Natamycin may be used as a sole additive or in combination with chemicals and / or microbial cultures to form mixed additives. The forage used may have original moisture content, or be partially dehydrated (withered) or added with moisture absorbers.
De acordo com o modelo, o aditivo contendo a natamicina poderá ser aplicado durante a produção da silagem, diluído em água, sendo aspergido na forragem picada antes ou durante a compactação no silo, ou ser aplicado de outra forma específica. A natamicina poderá ser misturada a veículos para facilitar a homogeneização e aspersão, como lactose, açúcares, pós e granulados químicos solúveis, entre outros, bem como culturas microbianas de lactobacilos ou outro microrganismos e/ou substâncias químicas. As dosagens aplicadas variam de 1 a 16 gramas de natamicina por tonelada de forragem picada, preferencialmente entre
2 e 8 g/†( mais preferencialmente entre 4 e 6 g/t, de acordo com o objetivo e potencial de deterioração dos alimentos. According to the model, the natamycin-containing additive may be applied during silage production, diluted with water, sprayed on chopped forage before or during compaction in the silo, or otherwise applied. Natamycin may be mixed with vehicles to facilitate homogenization and spraying such as lactose, sugars, soluble chemical powders and granulates, among others, as well as microbial cultures of lactobacilli or other microorganisms and / or chemicals. The dosages applied range from 1 to 16 grams of natamycin per tonne of chopped forage, preferably between 2 and 8 g / † ( more preferably between 4 and 6 g / t, according to the purpose and potential for food spoilage.
As demais etapas do processo de ensilagem não são alteradas ou influenciadas pela adoção da técnica de aplicação da natamicina no momento da ensilagem. The other steps of the ensiling process are not altered or influenced by the adoption of the natamycin application technique at the time of ensiling.
A natamicina é um antibiótico natural produzido à partir de cultura da bactéria Streptomyces natalensis e/ou outras espécies de Streptomyces. Apresenta estrutura cristalina e fórmula empírica C33H47N013, e atua associando-se com o ergosterol, 24 e 28- dehidroergosterol, e colesterol, presentes na membrana de bolores e leveduras, mas ausentes em bactérias, fazendo com que a natamicina seja um antibiótico potente inibidor de leveduras e bolores, porém sem efeito sobre bactérias. Dessa forma, o uso da natamicina como aditivo na produção de silagens permite exclusão seletiva de microrganismos indesejáveis no processo, como os fungos, bolores e leveduras, sem prejudicar o crescimento de bactérias desejáveis, sobretudo as bactérias acido-láticas. Natamycin is a natural antibiotic produced from culture of Streptomyces natalensis bacteria and / or other Streptomyces species. It has crystalline structure and empirical formula C33H47N013, and acts in association with ergosterol, 24 and 28-dehydroergosterol, and cholesterol, present in the membrane of molds and yeast, but absent in bacteria, making natamycin a potent antibiotic inhibitor. yeast and mold, but without effect on bacteria. Thus, the use of natamycin as an additive in silage production allows selective exclusion of undesirable microorganisms in the process, such as fungi, molds and yeast, without harming the growth of desirable bacteria, especially acid-lactic bacteria.
Funcionamento Operation
O funcionamento é simples e prático, bastando adicionar o aditivo contendo natamicina na forma sólida em pó, ou preferencialmente diluída em água e/ou outro solvente, durante o processo de confecção das silagens, onde a planta forrageira é picada, adicionada de natamicina e compactada em estrutura de armazenamento denominada silo, podendo esse silo apresentar forma e tamanho variável. A aplicação do aditivo contendo natamicina na forma sólida ou líquida ou outra pode ser feita manualmente ou mediante implementos desenvolvidos para esse fim, como regadores, bombas com aspersores, ou outros. A natamicina inibe o crescimento
de microrganismos indesejáveis durante as fases aeróbia e anaeróbia do processo fermentativo, bem como após a abertura dos silos. The operation is simple and practical, simply adding the additive containing natamycin in solid powder form, or preferably diluted with water and / or other solvent, during the silage preparation process, where the forage plant is minced, natamycin added and compacted. in a storage structure called silo, which silo may have variable shape and size. The application of the natamycin-containing additive in solid or liquid or other form may be done manually or by means of implements developed for this purpose, such as watering cans, spray pumps, or others. Natamycin inhibits growth undesirable microorganisms during the aerobic and anaerobic phases of the fermentation process, as well as after the opening of the silos.
Com tudo que foi exposto, o uso da natamicina em dosagens de 1 a 1 6 gramas de natamicina por tonelada de forragem picada, preferencialmente entre- 2 e 8 g/t, mais preferencialmente entre 4 e 6 g/t, torna-se um meio totalmente eficiente, prático e de boa relação entre custo e benefício, para melhorar a qualidade e elevar o valor nutritivo de silagens de plantas forrageiras, por inibir e reduzir as populações de microrganismos indesejáveis, tornando as silagens inoculadas mais estáveis quando expostas ao ar. Por essa razão, essa técnica deverá ser bem aceita pelos técnicos e produtores rurais em geral, que utilizam aditivos no processo de ensilagem.
With all of the above, the use of natamycin at dosages of 1 to 16 grams of natamycin per tonne of chopped forage, preferably between 2 and 8 g / t, more preferably between 4 and 6 g / t, becomes Fully efficient, practical and cost-effective means to improve quality and increase the nutritional value of forage plant silages by inhibiting and reducing undesirable microorganism populations, making inoculated silages more stable when exposed to air. For this reason, this technique should be well accepted by technicians and farmers in general, who use additives in the silage process.
Claims
1. )"NATAMICINA COMO ADITIVO NA PRODUÇÃO DE SILAGENS" †rata-se do uso do antimicótico NATAMICINA como aditivo na produção de silagens, é caracterizado pela aplicação da natamicina, na forma sólida ou líquida ou outra, pura ou diluída em veículos ou outros aditivos químicos ou microbianos, em dosagens de 1 a 16 gramas de natamicina por tonelada de forragem picada, preferencialmente entre 2 e 8 g/t, mais preferencialmente entre 4 e 6 g/t, durante a confecção de silagens de plantas forrageiras, com efeito de inibição de microrganismos indesejáveis nas fases aeróbia e anaeróbia do processo fermentativo, bem como após a exposição da silagem ao ar. 1.) "NATAMYCIN AS AN ADDITIVE IN SILAGE PRODUCTION" † The use of the antimycotic NATAMICIN as an additive in silage production is characterized by the application of natamycin, in solid or liquid or other form, pure or diluted in vehicles or other chemical or microbial additives, in dosages of 1 to 16 grams of natamycin per tonne of chopped forage, preferably between 2 and 8 g / t, more preferably between 4 and 6 g / t, during the manufacture of forage plant silages, in effect. inhibition of undesirable microorganisms in the aerobic and anaerobic phases of the fermentation process, as well as after exposure of silage to air.
2. )"NATAMICINA COMO ADITIVO NA PRODUÇÃO DE SILAGENS" de acordo com a reivindicação ( 1 ) é caracterizado pela aplicação de aditivo contendo natamicina no processo de produção de silagens, podendo ser aplicada na forma sólida ou líquida ou outra, podendo ser diluída em água ou outros veículos, associada ou não a outros aditivos, sendo aplicada manualmente ou mediante aplicador específico. 2.) "NATAMICIN AS A SILAGE PRODUCTION ADDITIVE" according to claim (1) is characterized by the application of a natamycin-containing additive in the silage production process, which may be applied in solid or liquid or other form and may be diluted in water or other vehicles, whether or not associated with other additives, being applied manually or by a specific applicator.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/BR2012/000084 WO2013063666A1 (en) | 2011-11-01 | 2012-03-23 | Use of natamycin as an additive for producing silage |
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| BRPROTOCOLO015110002408 | 2011-11-01 | ||
| PCT/BR2012/000084 WO2013063666A1 (en) | 2011-11-01 | 2012-03-23 | Use of natamycin as an additive for producing silage |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4536494A (en) * | 1983-11-17 | 1985-08-20 | Carter A Franklin | Animal feed method employing natamycin |
| US4600706A (en) * | 1983-11-17 | 1986-07-15 | Carter A F | Anti-fungal feed compositions containing natamycin |
| BR9307906A (en) * | 1992-01-28 | 1996-08-27 | Ducoa Lp | Animal feed additive Animal feed additive and animal feed composition |
| BR0008890A (en) * | 1999-01-21 | 2002-01-08 | Ducoa Lp | Method of reducing the incidence of ascites disease in birds |
-
2012
- 2012-03-23 WO PCT/BR2012/000084 patent/WO2013063666A1/en active Application Filing
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4536494A (en) * | 1983-11-17 | 1985-08-20 | Carter A Franklin | Animal feed method employing natamycin |
| US4600706A (en) * | 1983-11-17 | 1986-07-15 | Carter A F | Anti-fungal feed compositions containing natamycin |
| BR9307906A (en) * | 1992-01-28 | 1996-08-27 | Ducoa Lp | Animal feed additive Animal feed additive and animal feed composition |
| BR0008890A (en) * | 1999-01-21 | 2002-01-08 | Ducoa Lp | Method of reducing the incidence of ascites disease in birds |
Non-Patent Citations (1)
| Title |
|---|
| MICHAEL K. WOOLFORD: "Microbiological screening of food preservatives, cold sterilants and specific antimicrobial agents as potential Silage Additives", JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, vol. 26, no. ISS. 2, February 1975 (1975-02-01), pages 229 - 237, XP002505591 * |
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