WO2013021448A1 - Tube de mesure de fluorescence et appareil de mesure de fluorescence utilisant celui-ci - Google Patents

Tube de mesure de fluorescence et appareil de mesure de fluorescence utilisant celui-ci Download PDF

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Publication number
WO2013021448A1
WO2013021448A1 PCT/JP2011/068053 JP2011068053W WO2013021448A1 WO 2013021448 A1 WO2013021448 A1 WO 2013021448A1 JP 2011068053 W JP2011068053 W JP 2011068053W WO 2013021448 A1 WO2013021448 A1 WO 2013021448A1
Authority
WO
WIPO (PCT)
Prior art keywords
tube
fluorescence
fluorescence measuring
light
plate
Prior art date
Application number
PCT/JP2011/068053
Other languages
English (en)
Japanese (ja)
Inventor
幸吉 井戸
Original Assignee
エレコン科学株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by エレコン科学株式会社 filed Critical エレコン科学株式会社
Priority to PCT/JP2011/068053 priority Critical patent/WO2013021448A1/fr
Publication of WO2013021448A1 publication Critical patent/WO2013021448A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0854—Double walls

Definitions

  • the present invention relates to a fluorescence measuring tube mainly used for amplifying a prion and a fluorescence measuring instrument using the same.
  • tubes for storing tissues and cells have been widely used for cell proliferation and tissue and cell component separation.
  • these tubes have a shape that is narrowed downward and has a curved lower end.
  • these tubes were made of a transparent material so that the internal structure and the like could be visually recognized.
  • Such a tube is also used as a measurement tube for prion amplification.
  • the transparent tube was measured by applying light from below and receiving the light from above. In this case, it was limited to the case of measuring the spectrum.
  • the entire tube is composed of a black light shielding body to prevent light leakage, and light is irradiated from above the object to be inspected, thereby measuring the fluorescence emitted from above.
  • a black light shielding body to prevent light leakage, and light is irradiated from above the object to be inspected, thereby measuring the fluorescence emitted from above.
  • Some are used in some types of equipment.
  • both the cylindrical portion and the bottom portion are made of a light shielding body, and the shape is the same as that of the tube described in Patent Document 1.
  • the tube as described above can only be used as a tube for fluorescence measurement, and has a problem that it is not effective for measurement using ultrasonic waves because the bottom is curved.
  • the present invention solves such problems of the conventional example, and uses a fluorescence measurement tube that can be used for both fluorescence measurement and spectrum measurement, and can also be measured using ultrasonic waves, and the same.
  • An object is to provide a fluorescence measuring instrument.
  • the fluorescent measurement tube of the present invention is characterized in that the cylindrical portion is made of a light shielding body and the bottom portion of the cylindrical portion is made of a light transmitting body.
  • the fluorescent measurement tube of the present invention is characterized in that the cylindrical portion is formed of a light shielding body and the bottom portion of the cylindrical portion is formed of a flat translucent body.
  • the tube portion is formed of a light shielding body, and the bottom portion of the tube portion is formed of a flat translucent body. A pin for locking to the plate is provided.
  • the cylindrical portion is constituted by the light shielding body and the bottom portion of the cylindrical portion is constituted by the light transmitting body, in a normal state, light is taken from the bottom portion and irradiated on the inspection object, and the light is irradiated upward It can be used for measurement. Further, if the bottom is placed on the light shielding body, light does not enter from the bottom, so that it is possible to perform fluorescence measurement by irradiating the inspection object with light from above and measuring the emitted fluorescence upward.
  • the fluorescence measurement tube is immersed in water by arranging the fluorescence measurement tube continuously on the support, providing pins on both sides of the support, and locking the pins to the plate.
  • the fluorescence measuring tube can be prevented from lifting due to the buoyancy of water.
  • the partially expanded sectional front view of one Embodiment of the fluorescence measuring device of this invention The top perspective view of one embodiment of the fluorescence measuring instrument of the present invention
  • the bottom perspective view of one embodiment of the fluorescence measuring instrument of the present invention Explanatory drawing which shows the attachment position to the plate of the fluorescence measuring instrument of this invention
  • Explanatory drawing which shows the state which installed the fluorescence measuring device of this invention in the processing tank
  • Explanatory drawing which shows the state which installed the fluorescence measuring device of this invention in the plate folder
  • Explanatory drawing which shows the state which installed the fluorescence measuring device of this invention in the plate folder
  • the schematic diagram which shows the measurement state by the fluorescence measuring device of this invention The schematic diagram which shows the measurement state by the fluorescence measuring device of this invention
  • the fluorescence measurement tube (hereinafter referred to as tube) 1 of the present embodiment is made of a synthetic resin having a height of about 20 mm and a diameter of about 5 mm, but its size, material, etc. can be arbitrarily determined.
  • the tube 1 is composed of a cylindrical part 2 and a bottom part 3, and the cylindrical part 2 is constituted by a double structure of an outer cylinder 21 made of a black light shielding body and an inner cylinder 22 made of a transparent material. It has high sealing properties and excellent light shielding properties.
  • the bottom part 3 of the cylinder part 2 is a bottom part of the inner cylinder 22, and is comprised only by the transparent material which is a translucent body.
  • this bottom part 3 is attached to the cylinder part 2 and attached, there is a possibility of peeling when irradiated with ultrasonic waves, so a double structure is adopted.
  • FIGS. 2 and 3 eight tubes 1 are arranged at equal intervals, a support 5 is provided at the upper end, and the tube 1 is fixed to produce a fluorescence measuring instrument 10. Pins 6 for locking in locking holes 81 of a plate 8 to be described later are formed at the lower ends of both ends of the support 5.
  • a plate 8 having a plurality of rows of eight insertion holes 82 provided continuously on the surface is formed, and a locking hole for locking the pin 6 on both sides of the insertion hole 82. 81 is provided.
  • Reference numeral 11 denotes a lid of the tube 1.
  • a test object A or B such as a prion is placed on the bottom 3 of the tube 1, and the tube 1 is placed in the insertion hole 82 of the plate 8. Then, the pin 6 is locked in the locking hole 81 and the fluorescence measuring instrument 10 is fixed to the plate 8. And the said plate 8 is installed in the upper end of the processing tank 50, and ultrasonic waves are irradiated to the test object A or B by the ultrasonic generator 51.
  • the tube 1 is prevented from being lifted by the buoyancy of the water in the processing tank 50 and is stably measured. Can be done.
  • the plate 8 is removed from the processing tank 50 and transferred to the optical system, and measurement is performed with the plate folder 9 as shown in FIGS.
  • test object A is an amyloid-causing protein
  • irradiation with ultrasonic waves generates amyloid and emits fluorescence.
  • the fluorescence measuring instrument 10 is fixed to the plate holder 9 and the bottom 3 of the tube 1 is placed on the bottom plate 91 made of a light-shielding plate to prevent light from entering the cylinder portion 2 from the outside.
  • the inspection object A is irradiated with light by the light emitter 71, and the intensity of the fluorescence emitted by the amyloid is measured by the light receiver 70.
  • the fluorescence measuring instrument 10 when measuring the turbidity of a protein without performing fluorescence measurement, as shown in FIGS. 7 and 9, the fluorescence measuring instrument 10 has a bottom plate 92 provided with a large number of holes 93 on the surface. It fixes to the plate folder 9, arrange
  • prion protein in an early BSE-infected cow is amplified using an ultrasonic prion amplification device, and an immediate fluorescence measurement is performed without performing Western blotting, and a result is obtained in a few minutes. It can be done.
  • a prion amplified by ultrasonic waves can be used for both fluorescence measurement and spectrum measurement, and is widely used in the field of protein measurement, examination or research.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

La présente invention vise à fournir un tube de mesure de fluorescence, qui peut être utilisé à la fois dans les cas de mesure de fluorescence et de mesure de spectre, et qui est capable de réaliser une mesure à l'aide d'ondes ultrasonores, et de fournir un appareil de mesure de fluorescence utilisant le tube de mesure de fluorescence. A cet effet, selon l'invention, l'appareil de mesure de fluorescence est caractérisé en ce que : une pluralité de tubes de mesure de fluorescence (1) sont disposés en séquence sur un corps de support (5), chacun desdits tubes de mesure de fluorescence ayant une partie cylindrique (2) constituée d'un corps de blocage de lumière, et une partie inférieure (3) de la partie cylindrique (2) constituée d'un corps plat transmettant la lumière ; et des broches (6) (8) sont disposées sur les deux côtés des corps de support (5) pour se verrouiller à une plaque, ladite plaque comportant une pluralité de trous d'insertion (82) dans la surface.
PCT/JP2011/068053 2011-08-08 2011-08-08 Tube de mesure de fluorescence et appareil de mesure de fluorescence utilisant celui-ci WO2013021448A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/JP2011/068053 WO2013021448A1 (fr) 2011-08-08 2011-08-08 Tube de mesure de fluorescence et appareil de mesure de fluorescence utilisant celui-ci

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2011/068053 WO2013021448A1 (fr) 2011-08-08 2011-08-08 Tube de mesure de fluorescence et appareil de mesure de fluorescence utilisant celui-ci

Publications (1)

Publication Number Publication Date
WO2013021448A1 true WO2013021448A1 (fr) 2013-02-14

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016105090A (ja) * 2014-11-27 2016-06-09 ハッハ ランゲ ゲゼルシャフト ミット ベシュレンクテル ハフツングＨａｃｈ Ｌａｎｇｅ Ｇｍｂｈ 比濁分析濁度計

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60500426A (ja) * 1983-01-07 1985-03-28 ラブシステムズ　オイ 小容器セット
JPH10221243A (ja) * 1996-11-25 1998-08-21 Porvair Plc マイクロプレート及びマイクロプレート製造方法
JP2001509272A (ja) * 1997-01-17 2001-07-10 コーニング インコーポレイテッド マルチウェルプレート

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60500426A (ja) * 1983-01-07 1985-03-28 ラブシステムズ　オイ 小容器セット
JPH10221243A (ja) * 1996-11-25 1998-08-21 Porvair Plc マイクロプレート及びマイクロプレート製造方法
JP2001509272A (ja) * 1997-01-17 2001-07-10 コーニング インコーポレイテッド マルチウェルプレート

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016105090A (ja) * 2014-11-27 2016-06-09 ハッハ ランゲ ゲゼルシャフト ミット ベシュレンクテル ハフツングＨａｃｈ Ｌａｎｇｅ Ｇｍｂｈ 比濁分析濁度計

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