WO2013021448A1 - Fluorescence measuring tube and fluorescence measuring apparatus using same - Google Patents
Fluorescence measuring tube and fluorescence measuring apparatus using same Download PDFInfo
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- WO2013021448A1 WO2013021448A1 PCT/JP2011/068053 JP2011068053W WO2013021448A1 WO 2013021448 A1 WO2013021448 A1 WO 2013021448A1 JP 2011068053 W JP2011068053 W JP 2011068053W WO 2013021448 A1 WO2013021448 A1 WO 2013021448A1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/025—Align devices or objects to ensure defined positions relative to each other
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0654—Lenses; Optical fibres
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0829—Multi-well plates; Microtitration plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0848—Specific forms of parts of containers
- B01L2300/0854—Double walls
Definitions
- the present invention relates to a fluorescence measuring tube mainly used for amplifying a prion and a fluorescence measuring instrument using the same.
- tubes for storing tissues and cells have been widely used for cell proliferation and tissue and cell component separation.
- these tubes have a shape that is narrowed downward and has a curved lower end.
- these tubes were made of a transparent material so that the internal structure and the like could be visually recognized.
- Such a tube is also used as a measurement tube for prion amplification.
- the transparent tube was measured by applying light from below and receiving the light from above. In this case, it was limited to the case of measuring the spectrum.
- the entire tube is composed of a black light shielding body to prevent light leakage, and light is irradiated from above the object to be inspected, thereby measuring the fluorescence emitted from above.
- a black light shielding body to prevent light leakage, and light is irradiated from above the object to be inspected, thereby measuring the fluorescence emitted from above.
- Some are used in some types of equipment.
- both the cylindrical portion and the bottom portion are made of a light shielding body, and the shape is the same as that of the tube described in Patent Document 1.
- the tube as described above can only be used as a tube for fluorescence measurement, and has a problem that it is not effective for measurement using ultrasonic waves because the bottom is curved.
- the present invention solves such problems of the conventional example, and uses a fluorescence measurement tube that can be used for both fluorescence measurement and spectrum measurement, and can also be measured using ultrasonic waves, and the same.
- An object is to provide a fluorescence measuring instrument.
- the fluorescent measurement tube of the present invention is characterized in that the cylindrical portion is made of a light shielding body and the bottom portion of the cylindrical portion is made of a light transmitting body.
- the fluorescent measurement tube of the present invention is characterized in that the cylindrical portion is formed of a light shielding body and the bottom portion of the cylindrical portion is formed of a flat translucent body.
- the tube portion is formed of a light shielding body, and the bottom portion of the tube portion is formed of a flat translucent body. A pin for locking to the plate is provided.
- the cylindrical portion is constituted by the light shielding body and the bottom portion of the cylindrical portion is constituted by the light transmitting body, in a normal state, light is taken from the bottom portion and irradiated on the inspection object, and the light is irradiated upward It can be used for measurement. Further, if the bottom is placed on the light shielding body, light does not enter from the bottom, so that it is possible to perform fluorescence measurement by irradiating the inspection object with light from above and measuring the emitted fluorescence upward.
- the fluorescence measurement tube is immersed in water by arranging the fluorescence measurement tube continuously on the support, providing pins on both sides of the support, and locking the pins to the plate.
- the fluorescence measuring tube can be prevented from lifting due to the buoyancy of water.
- the partially expanded sectional front view of one Embodiment of the fluorescence measuring device of this invention The top perspective view of one embodiment of the fluorescence measuring instrument of the present invention
- the bottom perspective view of one embodiment of the fluorescence measuring instrument of the present invention Explanatory drawing which shows the attachment position to the plate of the fluorescence measuring instrument of this invention
- Explanatory drawing which shows the state which installed the fluorescence measuring device of this invention in the processing tank
- Explanatory drawing which shows the state which installed the fluorescence measuring device of this invention in the plate folder
- Explanatory drawing which shows the state which installed the fluorescence measuring device of this invention in the plate folder
- the schematic diagram which shows the measurement state by the fluorescence measuring device of this invention The schematic diagram which shows the measurement state by the fluorescence measuring device of this invention
- the fluorescence measurement tube (hereinafter referred to as tube) 1 of the present embodiment is made of a synthetic resin having a height of about 20 mm and a diameter of about 5 mm, but its size, material, etc. can be arbitrarily determined.
- the tube 1 is composed of a cylindrical part 2 and a bottom part 3, and the cylindrical part 2 is constituted by a double structure of an outer cylinder 21 made of a black light shielding body and an inner cylinder 22 made of a transparent material. It has high sealing properties and excellent light shielding properties.
- the bottom part 3 of the cylinder part 2 is a bottom part of the inner cylinder 22, and is comprised only by the transparent material which is a translucent body.
- this bottom part 3 is attached to the cylinder part 2 and attached, there is a possibility of peeling when irradiated with ultrasonic waves, so a double structure is adopted.
- FIGS. 2 and 3 eight tubes 1 are arranged at equal intervals, a support 5 is provided at the upper end, and the tube 1 is fixed to produce a fluorescence measuring instrument 10. Pins 6 for locking in locking holes 81 of a plate 8 to be described later are formed at the lower ends of both ends of the support 5.
- a plate 8 having a plurality of rows of eight insertion holes 82 provided continuously on the surface is formed, and a locking hole for locking the pin 6 on both sides of the insertion hole 82. 81 is provided.
- Reference numeral 11 denotes a lid of the tube 1.
- a test object A or B such as a prion is placed on the bottom 3 of the tube 1, and the tube 1 is placed in the insertion hole 82 of the plate 8. Then, the pin 6 is locked in the locking hole 81 and the fluorescence measuring instrument 10 is fixed to the plate 8. And the said plate 8 is installed in the upper end of the processing tank 50, and ultrasonic waves are irradiated to the test object A or B by the ultrasonic generator 51.
- the tube 1 is prevented from being lifted by the buoyancy of the water in the processing tank 50 and is stably measured. Can be done.
- the plate 8 is removed from the processing tank 50 and transferred to the optical system, and measurement is performed with the plate folder 9 as shown in FIGS.
- test object A is an amyloid-causing protein
- irradiation with ultrasonic waves generates amyloid and emits fluorescence.
- the fluorescence measuring instrument 10 is fixed to the plate holder 9 and the bottom 3 of the tube 1 is placed on the bottom plate 91 made of a light-shielding plate to prevent light from entering the cylinder portion 2 from the outside.
- the inspection object A is irradiated with light by the light emitter 71, and the intensity of the fluorescence emitted by the amyloid is measured by the light receiver 70.
- the fluorescence measuring instrument 10 when measuring the turbidity of a protein without performing fluorescence measurement, as shown in FIGS. 7 and 9, the fluorescence measuring instrument 10 has a bottom plate 92 provided with a large number of holes 93 on the surface. It fixes to the plate folder 9, arrange
- prion protein in an early BSE-infected cow is amplified using an ultrasonic prion amplification device, and an immediate fluorescence measurement is performed without performing Western blotting, and a result is obtained in a few minutes. It can be done.
- a prion amplified by ultrasonic waves can be used for both fluorescence measurement and spectrum measurement, and is widely used in the field of protein measurement, examination or research.
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- Life Sciences & Earth Sciences (AREA)
- General Physics & Mathematics (AREA)
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- Clinical Laboratory Science (AREA)
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
[Problem] To provide a fluorescence measuring tube, which can be used in both the cases of measuring fluorescence and measuring spectrum, and is capable of performing measurement using ultrasonic waves, and to provide a fluorescence measuring apparatus using the fluorescence measuring tube.
[Solution] This fluorescence measuring apparatus is characterized in that: a plurality of fluorescence measuring tubes (1) are sequentially disposed on a supporting body (5), each of said fluorescence measuring tubes having a cylindrical portion (2) configured of a light blocking body, and a bottom portion (3) of the cylindrical portion (2) configured of a flat light transmitting body; and on both the sides of the supporting bodies (5), pins (6) for locking to a plate (8) are provided, said plate being provided with a plurality of insertion holes (82) in the surface.
Description
本発明は、主としてプリオン増幅用に用いられる蛍光測定チューブ及びそれを用いた蛍光測定器に関するものである。
The present invention relates to a fluorescence measuring tube mainly used for amplifying a prion and a fluorescence measuring instrument using the same.
従来より細胞の増殖、組織や細胞成分の分離の際に、組織や細胞を収納するためのチューブが広く用いられている。これらのチューブは、例えば特許文献１に記載されているように、下方に向かって細くなっていき、下端が湾曲した形状であった。さらにこれらチューブは内部の組織等が視認できるように透明材質で作られていた。
2. Description of the Related Art Conventionally, tubes for storing tissues and cells have been widely used for cell proliferation and tissue and cell component separation. As described in, for example, Patent Document 1, these tubes have a shape that is narrowed downward and has a curved lower end. Furthermore, these tubes were made of a transparent material so that the internal structure and the like could be visually recognized.
このようなチューブはプリオン増幅用の測定チューブとしても使用されている。この場合透明チューブは、チューブの下方から光を当てて上方でその光を受けて計るものであった。この場合はスペクトルを計る場合に限られていた。
Such a tube is also used as a measurement tube for prion amplification. In this case, the transparent tube was measured by applying light from below and receiving the light from above. In this case, it was limited to the case of measuring the spectrum.
一方、プリオン増幅用等に用いる蛍光測定チューブとしては、光の漏れを防ぐためにチューブ全体を黒色の遮光体で構成して、検査対象の上方から光をあてて、それによって光る蛍光を上方で計る形式の装置に使用されているものがある。
このようなチューブは、筒部と底部が共に遮光体で構成されていて、かつ形状は前記の特許文献１記載のチューブと同様であった。
上述のようなチューブは、蛍光測定用のチューブとしてしか使用できず、さらに底部が湾曲しているため超音波を使用した測定には効果的でないという問題点があった。 On the other hand, as a fluorescence measurement tube used for prion amplification or the like, the entire tube is composed of a black light shielding body to prevent light leakage, and light is irradiated from above the object to be inspected, thereby measuring the fluorescence emitted from above. Some are used in some types of equipment.
In such a tube, both the cylindrical portion and the bottom portion are made of a light shielding body, and the shape is the same as that of the tube described inPatent Document 1.
The tube as described above can only be used as a tube for fluorescence measurement, and has a problem that it is not effective for measurement using ultrasonic waves because the bottom is curved.
このようなチューブは、筒部と底部が共に遮光体で構成されていて、かつ形状は前記の特許文献１記載のチューブと同様であった。
上述のようなチューブは、蛍光測定用のチューブとしてしか使用できず、さらに底部が湾曲しているため超音波を使用した測定には効果的でないという問題点があった。 On the other hand, as a fluorescence measurement tube used for prion amplification or the like, the entire tube is composed of a black light shielding body to prevent light leakage, and light is irradiated from above the object to be inspected, thereby measuring the fluorescence emitted from above. Some are used in some types of equipment.
In such a tube, both the cylindrical portion and the bottom portion are made of a light shielding body, and the shape is the same as that of the tube described in
The tube as described above can only be used as a tube for fluorescence measurement, and has a problem that it is not effective for measurement using ultrasonic waves because the bottom is curved.
本発明は、このような従来例の課題を解決したもので、蛍光測定用とスペクトルを計測する場合の両方に使用でき、かつ超音波を使用した測定も可能な蛍光測定チューブ及びそれを用いた蛍光測定器を提供することを目的とする。
The present invention solves such problems of the conventional example, and uses a fluorescence measurement tube that can be used for both fluorescence measurement and spectrum measurement, and can also be measured using ultrasonic waves, and the same. An object is to provide a fluorescence measuring instrument.
本発明の蛍光測定チューブは、筒部を遮光体で構成し、前記筒部の底部を透光体で構成したことを特徴とする。
The fluorescent measurement tube of the present invention is characterized in that the cylindrical portion is made of a light shielding body and the bottom portion of the cylindrical portion is made of a light transmitting body.
本発明の蛍光測定チューブは、筒部を遮光体で構成し、前記筒部の底部をフラットな透光体で構成したことを特徴とする。
The fluorescent measurement tube of the present invention is characterized in that the cylindrical portion is formed of a light shielding body and the bottom portion of the cylindrical portion is formed of a flat translucent body.
本発明の蛍光測定器は、筒部を遮光体で構成し、前記筒部の底部をフラットな透光体で構成した蛍光測定チューブを支持体に連続して配置し、この支持体の両側に、プレートに係止するためのピンを設けたことを特徴とする。
In the fluorescence measuring instrument of the present invention, the tube portion is formed of a light shielding body, and the bottom portion of the tube portion is formed of a flat translucent body. A pin for locking to the plate is provided.
請求項１の発明によれば、筒部を遮光体で構成し筒部の底部を透光体で構成したから、通常の状態では底部から光を取り入れて検査対象に照射しその光を上方で計測する測定に用いることができる。また、底部を遮光体の上に置けば光が底部から侵入しないから、検査対象に上方から光をあてて、それによって光る蛍光を上方で計る蛍光測定が可能となる。
According to the first aspect of the present invention, since the cylindrical portion is constituted by the light shielding body and the bottom portion of the cylindrical portion is constituted by the light transmitting body, in a normal state, light is taken from the bottom portion and irradiated on the inspection object, and the light is irradiated upward It can be used for measurement. Further, if the bottom is placed on the light shielding body, light does not enter from the bottom, so that it is possible to perform fluorescence measurement by irradiating the inspection object with light from above and measuring the emitted fluorescence upward.
請求項２の発明によれば、底部をフラットに構成したため、超音波を使用した測定に効果的である。
According to invention of Claim 2, since the bottom part was comprised flat, it is effective for the measurement which uses an ultrasonic wave.
請求項３の発明によれば、蛍光測定チューブを支持体に連続して配置し、この支持体の両側にピンを設け、このピンをプレートに係止することで、蛍光測定チューブを水中に漬けた場合に、水の浮力による蛍光測定チューブの浮き上がりを防止できるという効果を奏する。
According to the invention of claim 3, the fluorescence measurement tube is immersed in water by arranging the fluorescence measurement tube continuously on the support, providing pins on both sides of the support, and locking the pins to the plate. In this case, the fluorescence measuring tube can be prevented from lifting due to the buoyancy of water.
本実施形態の蛍光測定チューブ（以下、チューブという）１は、高さ約２０ｍｍ、直径約５ｍｍの合成樹脂製であるが、その大きさや材質等は任意に決定できる。
図1に示すように、チューブ１は筒部２と底部３からなり、筒部２が黒色の遮光体からなる外筒２１と透明材からなる内筒２２の二重構造で構成されているため、高いシール性があると共に遮光性に優れている。
一方、筒部２の底部３は内筒２２の底部であり、透光体である透明材のみによって構成されている。
なお、この底部３を筒部２に張り合わせて取り付けると、超音波を照射した際にはがれる恐れがあるため、二重構造としたものである。 The fluorescence measurement tube (hereinafter referred to as tube) 1 of the present embodiment is made of a synthetic resin having a height of about 20 mm and a diameter of about 5 mm, but its size, material, etc. can be arbitrarily determined.
As shown in FIG. 1, thetube 1 is composed of a cylindrical part 2 and a bottom part 3, and the cylindrical part 2 is constituted by a double structure of an outer cylinder 21 made of a black light shielding body and an inner cylinder 22 made of a transparent material. It has high sealing properties and excellent light shielding properties.
On the other hand, thebottom part 3 of the cylinder part 2 is a bottom part of the inner cylinder 22, and is comprised only by the transparent material which is a translucent body.
In addition, when thisbottom part 3 is attached to the cylinder part 2 and attached, there is a possibility of peeling when irradiated with ultrasonic waves, so a double structure is adopted.
図1に示すように、チューブ１は筒部２と底部３からなり、筒部２が黒色の遮光体からなる外筒２１と透明材からなる内筒２２の二重構造で構成されているため、高いシール性があると共に遮光性に優れている。
一方、筒部２の底部３は内筒２２の底部であり、透光体である透明材のみによって構成されている。
なお、この底部３を筒部２に張り合わせて取り付けると、超音波を照射した際にはがれる恐れがあるため、二重構造としたものである。 The fluorescence measurement tube (hereinafter referred to as tube) 1 of the present embodiment is made of a synthetic resin having a height of about 20 mm and a diameter of about 5 mm, but its size, material, etc. can be arbitrarily determined.
As shown in FIG. 1, the
On the other hand, the
In addition, when this
図２、図３に示すように、前記チューブ１を８個等間隔に配置して上端に支持体５を設けてチューブ１を固定し蛍光測定器１０を作成する。前記支持体５の両端の下部には、後述のプレート８の係止孔８１に係止するためのピン６を構成してある。
As shown in FIGS. 2 and 3, eight tubes 1 are arranged at equal intervals, a support 5 is provided at the upper end, and the tube 1 is fixed to produce a fluorescence measuring instrument 10. Pins 6 for locking in locking holes 81 of a plate 8 to be described later are formed at the lower ends of both ends of the support 5.
一方、図４に示すように、表面に８個の挿入孔８２を複数列連続して設けたプレート８を作り、前記挿入孔８２の両側に、前記ピン６を係止するための係止孔８１を設ける。符号１１はチューブ１の蓋である。
On the other hand, as shown in FIG. 4, a plate 8 having a plurality of rows of eight insertion holes 82 provided continuously on the surface is formed, and a locking hole for locking the pin 6 on both sides of the insertion hole 82. 81 is provided. Reference numeral 11 denotes a lid of the tube 1.
上記のように構成した蛍光測定器１０の使用に際しては、図５に示すように、チューブ１の底部３上にプリオン等の検査対象Ａ又はＢを置き、チューブ１をプレート８の挿入孔８２に挿入して、ピン６を係止孔８１に係止して蛍光測定器１０をプレート８に固定する。
そして前記プレート８を処理槽５０の上端に設置し、超音波発生機５１によって検査対象Ａ又はＢに超音波を照射する。 When using thefluorescence measuring instrument 10 configured as described above, as shown in FIG. 5, a test object A or B such as a prion is placed on the bottom 3 of the tube 1, and the tube 1 is placed in the insertion hole 82 of the plate 8. Then, the pin 6 is locked in the locking hole 81 and the fluorescence measuring instrument 10 is fixed to the plate 8.
And thesaid plate 8 is installed in the upper end of the processing tank 50, and ultrasonic waves are irradiated to the test object A or B by the ultrasonic generator 51. FIG.
そして前記プレート８を処理槽５０の上端に設置し、超音波発生機５１によって検査対象Ａ又はＢに超音波を照射する。 When using the
And the
このときチューブ１の底部３がフラットに構成されているため、超音波の照射が効率的に行われる。また、蛍光測定器１０は両端のピン６によってプレート８の係止孔８１に係止して固定されているから、処理槽５０内の水の浮力によってチューブ１の浮きあがりが防止され安定した測定が行えるのである。
At this time, since the bottom 3 of the tube 1 is configured to be flat, ultrasonic irradiation is efficiently performed. Further, since the fluorescence measuring instrument 10 is locked and fixed to the locking holes 81 of the plate 8 by the pins 6 at both ends, the tube 1 is prevented from being lifted by the buoyancy of the water in the processing tank 50 and is stably measured. Can be done.
超音波の照射が行われた後、プレート８を処理槽５０から外して、光学系に移し、図６、図７に示すようにプレートフォルダ９で測定を行う。
After the irradiation of ultrasonic waves, the plate 8 is removed from the processing tank 50 and transferred to the optical system, and measurement is performed with the plate folder 9 as shown in FIGS.
蛍光測定を行う場合の実施例を図６、図８に基づいて説明する。検査対象Ａがアミロイド原因蛋白質である場合には、超音波を照射するとアミロイドが生成され蛍光を発する。
蛍光測定器１０をプレートフォルダ９に固定してチューブ１の底部３を遮光板からなる底板９１の上に置き、外部から筒部２内への光の侵入を防止して、チューブの蓋１１を外した後、検査対象Ａに発光機７１によって光を照射し、アミロイドの発する蛍光の強度を受光機７０によって測定するのである。 An embodiment in which fluorescence measurement is performed will be described with reference to FIGS. When the test object A is an amyloid-causing protein, irradiation with ultrasonic waves generates amyloid and emits fluorescence.
Thefluorescence measuring instrument 10 is fixed to the plate holder 9 and the bottom 3 of the tube 1 is placed on the bottom plate 91 made of a light-shielding plate to prevent light from entering the cylinder portion 2 from the outside. After the removal, the inspection object A is irradiated with light by the light emitter 71, and the intensity of the fluorescence emitted by the amyloid is measured by the light receiver 70.
蛍光測定器１０をプレートフォルダ９に固定してチューブ１の底部３を遮光板からなる底板９１の上に置き、外部から筒部２内への光の侵入を防止して、チューブの蓋１１を外した後、検査対象Ａに発光機７１によって光を照射し、アミロイドの発する蛍光の強度を受光機７０によって測定するのである。 An embodiment in which fluorescence measurement is performed will be described with reference to FIGS. When the test object A is an amyloid-causing protein, irradiation with ultrasonic waves generates amyloid and emits fluorescence.
The
これに対して、蛍光測定を行わず、蛋白質の濁度を計測する場合は、図７、図９に示すように、蛍光測定器１０を、表面に多数の穴９３を設けた底板９２を有するプレートフォルダ９に固定して、それぞれのチューブ１の底部３が穴９３の上方に位置するように配置して、チューブの蓋１１を外す。その後、底部３の下方から発光機７１によって検査対象Ｂに光を照射し、上方の受光機７０でその光を受けて濁度を計測するのである。
On the other hand, when measuring the turbidity of a protein without performing fluorescence measurement, as shown in FIGS. 7 and 9, the fluorescence measuring instrument 10 has a bottom plate 92 provided with a large number of holes 93 on the surface. It fixes to the plate folder 9, arrange | positions so that the bottom part 3 of each tube 1 may be located above the hole 93, and remove | eliminate the cover 11 of a tube. Thereafter, the inspection object B is irradiated with light by the light emitter 71 from below the bottom 3, and the turbidity is measured by receiving the light with the upper light receiver 70.
現在、ＢＳＥ検査では、エライザー法といわれる検査が一般的に行われており最終的にはウエスタンブロット法にて確定検査を行っている。
エライザー法は確実性に乏しい上に２０ヶ月以下の牛では使用できない。さらにウエスタンブロット法では結果がでるまで７日以上かかる。 Currently, in the BSE test, a test called the Eliser method is generally performed, and finally a final test is performed by the Western blot method.
The Eliza method is not reliable and cannot be used with cattle less than 20 months old. Furthermore, Western blotting takes more than 7 days to produce results.
エライザー法は確実性に乏しい上に２０ヶ月以下の牛では使用できない。さらにウエスタンブロット法では結果がでるまで７日以上かかる。 Currently, in the BSE test, a test called the Eliser method is generally performed, and finally a final test is performed by the Western blot method.
The Eliza method is not reliable and cannot be used with cattle less than 20 months old. Furthermore, Western blotting takes more than 7 days to produce results.
本実施形態の蛍光測定チューブを使用すると、超音波プリオン増幅装置を用いて初期のＢＳＥ感染牛の微量なプリオン蛋白質を増幅させウエスタンブロット法を行わないで即蛍光測定を行い数分で結果を出すことができるのである。
When the fluorescence measurement tube of the present embodiment is used, a very small amount of prion protein in an early BSE-infected cow is amplified using an ultrasonic prion amplification device, and an immediate fluorescence measurement is performed without performing Western blotting, and a result is obtained in a few minutes. It can be done.
本発明によれば、超音波で増幅したプリオンを蛍光測定する場合とスペクトルを計測する場合の両方に用いることができ、蛋白質の測定、検査あるいは研究の領域において広く利用される。
According to the present invention, a prion amplified by ultrasonic waves can be used for both fluorescence measurement and spectrum measurement, and is widely used in the field of protein measurement, examination or research.
１ チューブ
２ 筒部
３ 底部
５ 支持体
６ ピン
８ プレート
９ プレートフォルダ
２１ 外筒
２２ 内筒
５０ 処理槽
５１ 超音波発生機
Ａ 検査対象
Ｂ 検査対象 DESCRIPTION OFSYMBOLS 1 Tube 2 Cylinder part 3 Bottom part 5 Support body 6 Pin 8 Plate 9 Plate folder 21 Outer cylinder 22 Inner cylinder 50 Processing tank 51 Ultrasonic generator A Inspection object B Inspection object
２ 筒部
３ 底部
５ 支持体
６ ピン
８ プレート
９ プレートフォルダ
２１ 外筒
２２ 内筒
５０ 処理槽
５１ 超音波発生機
Ａ 検査対象
Ｂ 検査対象 DESCRIPTION OF
Claims (3)
- 筒部を遮光体で構成し、前記筒部の底部を透光体で構成した蛍光測定チューブ。 A fluorescence measuring tube in which a cylindrical portion is configured by a light shielding body and a bottom portion of the cylindrical portion is configured by a light transmitting body.
- 筒部を遮光体で構成し、前記筒部の底部をフラットな透光体で構成した蛍光測定チューブ。 A fluorescence measurement tube in which a cylindrical portion is configured by a light shielding body and a bottom portion of the cylindrical portion is configured by a flat light transmitting body.
- 筒部を遮光体で構成し、前記筒部の底部をフラットな透光体で構成した蛍光測定チューブを支持体に連続して配置し、
この支持体の両側に、プレートに係止するためのピンを設けたことを特徴とする蛍光測定器。 The tube portion is composed of a light-shielding body, and the fluorescence measurement tube composed of a flat translucent body at the bottom of the tube portion is continuously arranged on the support,
A fluorescence measuring instrument, wherein pins for locking to a plate are provided on both sides of the support.
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| PCT/JP2011/068053 WO2013021448A1 (en) | 2011-08-08 | 2011-08-08 | Fluorescence measuring tube and fluorescence measuring apparatus using same |
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| PCT/JP2011/068053 WO2013021448A1 (en) | 2011-08-08 | 2011-08-08 | Fluorescence measuring tube and fluorescence measuring apparatus using same |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016105090A (en) * | 2014-11-27 | 2016-06-09 | ハッハ ランゲ ゲゼルシャフト ミット ベシュレンクテル ハフツングＨａｃｈ Ｌａｎｇｅ Ｇｍｂｈ | Nephelometric turbidimeter |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60500426A (en) * | 1983-01-07 | 1985-03-28 | ラブシステムズ オイ | small container set |
| JPH10221243A (en) * | 1996-11-25 | 1998-08-21 | Porvair Plc | Microplate and its manufacture |
| JP2001509272A (en) * | 1997-01-17 | 2001-07-10 | コーニング インコーポレイテッド | Multi-well plate |
-
2011
- 2011-08-08 WO PCT/JP2011/068053 patent/WO2013021448A1/en active Application Filing
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60500426A (en) * | 1983-01-07 | 1985-03-28 | ラブシステムズ オイ | small container set |
| JPH10221243A (en) * | 1996-11-25 | 1998-08-21 | Porvair Plc | Microplate and its manufacture |
| JP2001509272A (en) * | 1997-01-17 | 2001-07-10 | コーニング インコーポレイテッド | Multi-well plate |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016105090A (en) * | 2014-11-27 | 2016-06-09 | ハッハ ランゲ ゲゼルシャフト ミット ベシュレンクテル ハフツングＨａｃｈ Ｌａｎｇｅ Ｇｍｂｈ | Nephelometric turbidimeter |
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