WO2013010368A1 - Utilisation de gène clé de formation d'aérenchyme de riz oslsd2 - Google Patents

Utilisation de gène clé de formation d'aérenchyme de riz oslsd2 Download PDF

Info

Publication number
WO2013010368A1
WO2013010368A1 PCT/CN2011/084098 CN2011084098W WO2013010368A1 WO 2013010368 A1 WO2013010368 A1 WO 2013010368A1 CN 2011084098 W CN2011084098 W CN 2011084098W WO 2013010368 A1 WO2013010368 A1 WO 2013010368A1
Authority
WO
WIPO (PCT)
Prior art keywords
rice
gene
oslsd2
nitrogen
plant
Prior art date
Application number
PCT/CN2011/084098
Other languages
English (en)
Chinese (zh)
Inventor
范晓荣
徐国华
朱静雯
Original Assignee
南京农业大学
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 南京农业大学 filed Critical 南京农业大学
Publication of WO2013010368A1 publication Critical patent/WO2013010368A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Definitions

  • the invention belongs to the technical field of ⁇ engineering and relates to the application of rice ventilating tissue to form key genes.
  • Nitrogen is one of the most important nutrient elements in crops and participates in various metabolic processes in organisms. It is a component of many living substances in plants, such as: amino acids, proteins, nucleic acids, enzymes, chlorophyll and so on. Nitrogen accounts for 1.5% of the total dry weight of the plant and 16% of the total protein of the plant (Frink CR., Waggoner PE. and Ausubel JH. Nitrogen fertilizer: retrospect and prospect. Proc. Nati. Acad. Sci. USA. 1999. 96 : 1175 ⁇ : 1180. ).
  • rice is suitable for ammonium crops
  • studies have shown that a certain amount of nitrate nitrogen can promote the absorption of ammonium by rice, and in the late stage of rice growth and development, rice is mainly composed of nitrate nitrogen.
  • the root development of rice is regulated by nitrate.
  • the mechanism by which the main nitrate promotes root growth is that nitrate regulates the transport distribution of auxin.
  • molecular biology technology has developed rapidly, providing new tools for crop genetics and breeding. People also attach great importance to the use of biological methods to improve the utilization of nitrogen fertilizer to solve the problem of nitrogen fertilizer.
  • the oxygen-secretion process of rice roots is an essential process for its growth in an anaerobic environment.
  • This may be related to the xyloglucan transglucosidase xei gene, the pyruvate decarboxylase pcfe gene, the glycerol phosphate dehydrogenase gpc gene, etc. (Subbaiah fe Sachs, 2003).
  • the object of the present invention is achieved by the following technical scheme - the application of the key gene 0 S L502 formed by rice control aeration tissue in increasing plant height and increasing plant nitrogen use efficiency and/or yield, the sequence accession number of which is AK111759.
  • the plant is preferably a monocotyledonous plant, and further preferably rice, corn or wheat, and particularly preferably rice.
  • Rice encodes a key gene for the formation of aergic tissue, the coding product of OSSLD2, rice aerenchyma, forming key protein OSLSD2 Application in increasing plant height, increasing plant nitrogen use efficiency and/or yield.
  • the present invention provides the biological function of the rice auxin transport protein gene OSLSD2 for the first time through systematic research.
  • the OSLSD2 overexpression material obtained by the transgenic method of the present invention causes a large increase in OSLSD2 expression in leaves (Fig.
  • the transgenic material obtained higher plants, which is beneficial to the absorption and utilization of nutrients, especially nitrogen, and nitrogen use efficiency.
  • Figure 1 shows the expression characteristics of OSLSD2 in different parts of rice (leaf, root), of which h root 2 t leaves.
  • FIG. 2 The OSLSD2 overexpression material (0-1, 0-2 0-3, 0-4) was identified, and the expression of four overexpressing lines in leaves was significantly enhanced compared with wild type.
  • WT wild type
  • CK blank control
  • 0-1, 0-2, 0-3, 0-4 respectively represent 0sLSD2 overexpression material.
  • Figure 4 OSLSD2 overexpression material (04) increased plant height and number of tillers compared to wild type (Wuyu ⁇ 7).
  • WT wild type, 0-4 : indicates 0SLSD2 over-expression material
  • the right picture is a microscopic picture of aeration tissue at a distance of 1 cm from the apex
  • the left side is a microscopic picture of a ventilated tissue at a distance of 1.5 cm from the apex.
  • RNA was extracted from the rice seedlings of Wuyujing No. 7 by a mass ratio of 30% NaCL0 2 to be sterilized, germinated, and cultured to the second leaf and one heart, and the rice plants of the same size were selected, and the endosperm was removed and transplanted to PH 5. 5 In the IRRI nutrient solution of the International Rice Research Institute, the four-leaf one-day-in-one is replaced by the International Rice Research Institute. (Ma.o D R. The methods of plant nutri tion research .
  • Bei jing ⁇ ⁇ Bei jing Agri cultural University ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ 5 ⁇ isopropyl ⁇ , centrifugation and discarding, adding 1 ml of Trizol reagent, adding 0.2 mL of chloroform, centrifuging, and taking up the supernatant, adding 0.5 mL of isopropanol.
  • RNA quality was detected by agarose gel electrophoresis at a mass ratio of 1.7, and the concentration and purity of total IA were measured by spectrophotometer. ;
  • the PCR reaction system is 20 ⁇ 1, including 10 pmol L - 1 positive and reverse primers each lu L, 10s PCR buffer 2 ⁇ L, 2.5 mM dNTP 1.6 ⁇ L, Taq enzyme 0, 4 L, and then supplemented with sterilized water 20 ti L (The primers were synthesized by Nanjing Jinsirui Company; the PCR reagents were purchased from Takara Company, Dalian).
  • the amount of template added varies according to its concentration, and is corrected by the amount of cytoskeletal protein gene (O ci ) of the internal reference gene rice.
  • the PCR program of the gene Octin is as follows: pre-denaturation at 94 °C for 4 min, denaturation at 94 °C for 30 s.
  • the renaturation was carried out at 55 °C for 30 s, at 72 °C for 30 s, after 30 cycles, and at 72 °C for 7 min, the amplified PCR products were detected by mass ratio 1.5% agarose gel electrophoresis. After staining with ABC (EB), imaging was performed in a gel imaging system.
  • the serial number and primer design of the gene are as follows:
  • PCR primers were designed using the total cDNA of rice cultivar Wuyujing 7 obtained above as a template.
  • the PCR product contained the complete 0sLSD2 reading frame (from the start codon ATG to the 3' non-coding region).
  • the primer sequence was:
  • 0verLSD2-F 5, - TTGAGGATCCGTGCCATTTACACCTC- 3 ' (SEQ ID NO. 5, containing BaniH I restriction site)
  • 0verLSD2- 5, - ATATGGT 'ACCACAGACCTTGCGCCAT-3, (SEQ ID NO. 6, containing Kpn: I enzyme Cut site)
  • PCR 20 system 2 ⁇ L 2, 5 mM dNTP, 2 uL l Ox PGR buffer , lu L 0verLSD2-F, 1 ⁇ L 0verLSD2-R, 1 u L cDNA, 13 ⁇ L dd3 ⁇ 40.
  • the PCR procedure was as follows: pre-denaturation at 94 °C for 4 min, denaturation at 98 °C for 10 s, refolding at 68 °C for 2 min, after 3Q cycles, 72 V for 10 min, the amplified PCR product passed the mass ratio of 1% agarose.
  • the total volume of the enzyme system was 10 UL, including 5 ⁇ L of the ligation solution.
  • the correct bacterial solution was added to an equal volume volume of 30% glycerol and stored at -70 ⁇ , and a recombinant plasmid containing the full-length sequence of the target gene 0sLSD2 cDNA was obtained, which was named 0LSD2-T. ;
  • 0LSD2-T plasmid obtained above as a template, 0verLSD2-F (SEQ ID NO. 5) and C) verLSD2-R (SEQ ID NO. 6) as primers, PCR amplification of the siS 2 gene fragment, the PCR procedure is as follows: Pre-denaturation at 94 °C: 4 min, denaturation at 98 °C for 10 s, 68 renaturation for 2 min, after 30 cycles, 72 "C for 10 min, amplified PCR products were detected by mass ratio 1% agarose gel electrophoresis The size of the PCR product was 822 bp. The target PCR product was separated by agarose electrophoresis and then recovered by gel digestion.
  • the recovered product was digested with restriction endonucleases Ba HI and Kpn I, and the double-excision plant overexpression vector PCAMBIA13Q0 (
  • the plasmid was purchased from Biovector Science Lab Co., and the digested PCR fragment and vector were separately recovered, and the vector was dephosphorylated and recovered again.
  • the linearized vector and the enzyme-cut PCR were recovered by T4 ligase after recovery. The fragment was ligated overnight at 4 °C, transformed into DH5 a competent cells of Bacillus megaterium, and plated on LB solid medium containing kanamycin 50 and gmL-l for 12 h, then picked positive colonies and extracted.
  • the plasmid was digested with BamH I and Kpn I to verify the size of the fragment, and the strain was The liquid was subjected to DM sequencing, and the bacterial liquid containing the correctly cloned clone was added to an equal volume volume of 30% glycerol and stored at -70 ,, and the positive cloned plasmid was named PUM-LSD2;
  • the P Ubi-LSD2 plasmid was transformed into competent cells of Agrobacterium tumefaciens EHA105 by electroporation, and plated on YEP solid medium containing kanamycin and streptomycin both at 50 ⁇ g mL- 1 for 48 h. After that, pick positive colonies and extract the plasmid. After the B a .nfl l, Kpn l digestion was verified, the bacterial solution was added to an equal volume volume ratio of 30 ⁇ . Glycerol was stored at - 70 'C, and the transgene was spared.
  • the Agrobacterium transformed with the pUbi LSD2 plasmid obtained above was infested with the callus of Wuyujing 7 and co-cultured for 60 days. After selection, culture, differentiation, rooting and smelting, the transgenic plants were obtained.
  • 6-BA 6-benzyl adenine
  • Car penicillin
  • NAA naphthaleneacetic acid
  • IAA indoleacetic acid
  • 2, 4 -D 2,4-dichlorophenoxyacetic acid
  • AS acetosyringone
  • CH hydrolyzed casein
  • L-pro L-valine
  • L-Glu L-glutamine
  • MES 2-morpholineethanesulfonic acid
  • N6 N6 mass element solution
  • B5 BS trace element solution
  • AA AA mass element
  • Agar agar
  • N6 macro element N6 macro element.; 50 ml B5 trace.; 10 ml iron salt: 10 ml niacin: 1 ml pyridoxine hydrochloride: I ml thiamine hydrochloride: 1 ml inositol: 2 g MES: 3. 9 g sucrose 30 g
  • Resistant callus selection medium (per liter content):
  • N6 large element 50 ml B5 trace : 10 ml iron salt t 10 ml niacin: 1 ml pyridoxine hydrochloride: 1 ml thiamine hydrochloride: 1 ml inositol: 10 ml L-Glu : 0. 5 g L- Pro : 0. 5 g CH : 0. 3 g 2, 4-D : 8 ml maltose./sucrose 30 g Phytagel : 4. 0 g pH: 5. 8
  • Rooting medium formula (per liter content);
  • AAM Medium formula (AAM) of suspension of Agrobacterium-infected callus mass (per liter content) : AA A large number of elements: 100 ml B5 Trace: 10 ml Iron salt: 10 ml Niacin: 1 ml Pyridoxine hydrochloride: 1 ml Thiamine hydrochloride: 1 ml Inositol: 10 ml MES: 3. 9 g CH : 0. 5 g maltose - 30 g pH: 5. 5
  • Induction of callus Peeled rice seeds (14 tablets in one plate) into a triangular flask, soaked for 1 min in volume with 70% ethanol (submerged seeds), poured out 70% ethanol by volume, soaked with 30% by volume of sodium hypochlorite Min, then wash 5-6 times with sterile water until clear. Use a pair of tweezers to place the seeds on the sterilized filter paper, blot the water, and finally place the Wuyujing 7 seed on the induction medium and incubate in a 30 °C light incubator for 20-30 days.
  • Subculture Select the yellow, tough, detached callus of millet size and transfer to a subculture medium for 7-14 days with sterile forceps.
  • Agrobacterium 20 ⁇ L of Agrobacterium EM105 stock solution transformed with pUbi-LSD2 plasmid was inoculated into 5 ml of YEP containing 50 mg of L- 1 streptomycin and SO mgL- 1 kanamycin ( Sambrook , et al . Molecular Cloning Experimental Guide. SOOl) In a liquid medium, shake at 28 °C overnight. 5 ⁇ The activated bacteria liquid 500 L, inoculated in 5 ml of the same YEP medium containing the same antibiotics, continue to culture until the absorbance of the bacterial solution at a wavelength of 600 nm (0D600) 0. 8-1.
  • Infecting callus and co-cultivation The cotton callus of Wuyujing No. 7 was picked from the subculture medium and placed in a centrifuge tube. The amount of callus was less than 50 ml of the cone of the centrifuge tube. (Choose a light yellow rounded tough callus). Take the cultured Agrobacterium liquid solution. ml in a centrifuge tube at 4 V, 5000 rpm, centrifuge for 1 min, and remove the supernatant. The collected cells were suspended in a medium (AAM) containing 30 ml of suspension of Agrobacterium tumefaciens containing 200 mol of L- 1 acetosyringone (As), and the suspension was poured into the picked callus.
  • AAM medium
  • First round of screening Transfer the dried callus to a selection medium containing 250 mg L, 1 carbenicillin (car) and 50 rng L' 1 hygromycin (Hyg) for the first selection, 30 V , light culture 14 d; a second round of screening: an initial long-resistant callus callus to 250 mgL- 1 containing carbenicillin (CAR) and 80 mgL- 1 hygromycin (Hyg) selection On the medium, 30. C, light culture for 10 days and then transferred to the tissue culture room for 4 days.
  • a selection medium containing 250 mg L, 1 carbenicillin (car) and 50 rng L' 1 hygromycin (Hyg) for the first selection, 30 V , light culture 14 d
  • a second round of screening an initial long-resistant callus callus to 250 mgL- 1 containing carbenicillin (CAR) and 80 mgL- 1 hygromycin (Hyg) selection
  • CAR carbenicillin
  • Hyg hygromycin
  • the resistant callus of fresh yellow color is picked into the differentiation tank containing the differentiation medium, placed in a constant temperature culture chamber, and then differentiated into seedlings (about 30 days, The culture conditions of the tissue culture chamber were 24-30 V, 14 h light/8 h dark), and the seedlings were grown to a root length of about 5 cm.
  • breeding and transplanting of transgenic seedlings Pick out the tube with the better roots and stems and leaves. (The seedling grows to the top of the tube, it should be covered in time), open the sealing membrane, add appropriate amount of sterile water (prevent the medium length Bacteria), refining the seedlings for about 3 to 7 days, then washing off the agar, transplanting it to the greenhouse for hydroponic or soil culture growth and testing.
  • the wild-type (Wuyu ⁇ 7) and OSLSD2 over-expressing material (0-4) seedling roots ie, the first roots after seed germination
  • the root tips were 1-1. 5 cm, 1. 5-2 c two roots, paraffin sections were prepared, and the aerenchyma of the roots was observed under a microscope.
  • the results are shown in Fig. 5.
  • the OSLSD2 overexpression material can be seen from Fig. 5. (0-4)
  • the root aeration tissue is developed compared with the wild type (Wuyu ⁇ 7).
  • the experimental site is Ledong County, Hainan province. The time is from December 2010 to May 2011.
  • the experimental materials are Wuyujing 7 wild type and 0SLSD2 T2 generation genetically modified material.
  • the specific experimental implementation process is as follows:
  • Fertilizer application 1 leaf 1 heart fertilization on the trampoline 5 kg urea / mu; after 5-6 days tiller, compound fertilizer 8 kg + 7 kg urea / mu; 3 days before the seedlings urea 15 kg / mu;
  • transplanting rice On January 25th, transplanting rice, transplanting '2-3 days base fertilizer 60 kg compound fertilizer/mu, February 1 application day additional herbicide and returning green fertilizer nitrogen fertilizer 15 kg urea per mu, February 7 additional tiller fertilizer nitrogen fertilizer 15 kg urea Per acre.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

L'invention concerne l'utilisation d'un gène clé de formation d'aérenchyme de riz OsLSD2 (déméthylase spécifique de la lysine d'Oryza sativa). Le gène, dont le numéro d'accession est AK111759，peut être utilisé pour améliorer la hauteur, l'efficacité d'utilisation de l'azote et/ou la production des plantes. De manière plus spécifique, le riz est préparé avec le gène OsLSD2 surexprimé dans les racines et les feuilles par une méthode génétique, l'aérenchyme de racine de plante de riz étant modifié. Par rapport au type sauvage, les plantes transgéniques sont plus grandes, et l'efficacité d'utilisation de l'azote et la production d'une seule plante sont augmentées d'au moins 30%.
PCT/CN2011/084098 2011-07-18 2011-12-16 Utilisation de gène clé de formation d'aérenchyme de riz oslsd2 WO2013010368A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201110200437A CN102260709B (zh) 2011-07-18 2011-07-18 水稻通气组织形成关键基因OsLSD2 的应用
CN201110200437.6 2011-07-18

Publications (1)

Publication Number Publication Date
WO2013010368A1 true WO2013010368A1 (fr) 2013-01-24

Family

ID=45007544

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2011/084098 WO2013010368A1 (fr) 2011-07-18 2011-12-16 Utilisation de gène clé de formation d'aérenchyme de riz oslsd2

Country Status (2)

Country Link
CN (1) CN102260709B (fr)
WO (1) WO2013010368A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102260709B (zh) * 2011-07-18 2012-10-03 南京农业大学 水稻通气组织形成关键基因OsLSD2 的应用
CN105132428B (zh) * 2015-09-22 2018-05-04 中国农业大学 一种与植物根系性状相关的ZmLRT基因及其相关生物材料与应用
CN110923241B (zh) * 2019-11-05 2022-10-14 南京农业大学 水稻控制通气组织形成的关键基因OsLSD1.1在减少甲烷排放上的应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1451749A (zh) * 2003-05-13 2003-10-29 中国科学院微生物研究所 一种负调控植物程序性细胞死亡和促进转基因愈伤组织生长分化的水稻锌指蛋白基因
CN101392257A (zh) * 2008-11-10 2009-03-25 南京农业大学 水稻硝酸盐运输蛋白基因组OsNRT2.3的基因工程应用
WO2009114733A2 (fr) * 2008-03-13 2009-09-17 Ceres, Inc. Séquences nucléotidiques et polypeptides correspondants conférant un taux de croissance et une biomasse modulés dans des plantes cultivées dans des conditions salines et oxydantes
WO2010139784A1 (fr) * 2009-06-05 2010-12-09 Universitätsklinikum Freiburg Déméthylase spécifique de la lysine 1 (lsd1) comme biomarqueur du cancer du sein
CN102260709A (zh) * 2011-07-18 2011-11-30 南京农业大学 水稻通气组织形成关键基因OsLSD2 的应用

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1451749A (zh) * 2003-05-13 2003-10-29 中国科学院微生物研究所 一种负调控植物程序性细胞死亡和促进转基因愈伤组织生长分化的水稻锌指蛋白基因
WO2009114733A2 (fr) * 2008-03-13 2009-09-17 Ceres, Inc. Séquences nucléotidiques et polypeptides correspondants conférant un taux de croissance et une biomasse modulés dans des plantes cultivées dans des conditions salines et oxydantes
CN101392257A (zh) * 2008-11-10 2009-03-25 南京农业大学 水稻硝酸盐运输蛋白基因组OsNRT2.3的基因工程应用
WO2010139784A1 (fr) * 2009-06-05 2010-12-09 Universitätsklinikum Freiburg Déméthylase spécifique de la lysine 1 (lsd1) comme biomarqueur du cancer du sein
CN102260709A (zh) * 2011-07-18 2011-11-30 南京农业大学 水稻通气组织形成关键基因OsLSD2 的应用

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK 4 December 2008 (2008-12-04), accession no. AG99402 *
DATABASE GENBANK 4 December 2008 (2008-12-04), accession no. K111759 *
JOSHI, ROHIT ET AL.: "Hypoxia induced non-apoptotic cellular changes during aerenchyma formation in rice (Oryza sativa L.) roots", PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS, vol. 16, no. 1, 31 January 2010 (2010-01-31), pages 99 - 106 *
YIN, CHUNYUAN ET AL.: "Differences in Nitrogen Absorption and Use Efficiency in Rice Genotypes with Different Yield Performance", SCIENTIA AGRICULTURA SINICA, vol. 43, no. 1, 31 January 2010 (2010-01-31), pages 39 - 50 *

Also Published As

Publication number Publication date
CN102260709A (zh) 2011-11-30
CN102260709B (zh) 2012-10-03

Similar Documents

Publication Publication Date Title
CN106868021B (zh) 控制水稻种子大小基因OsNAC1及其应用
WO2022135246A1 (fr) Gène r pour commander l'appariement du soja-rhizobium, protéine et son utilisation
CN110904071B (zh) Raf49蛋白及其编码基因在调控植物抗旱性中的应用
CN112522291B (zh) 水稻OsSH3P2基因及其应用
WO2020221029A1 (fr) Gène zmrlk7 du type récepteur de zea mays et son utilisation
CN108624596B (zh) 一种调控豆科根瘤生长的基因GmSPX5及其应用
CN110643630B (zh) Knat1基因在提高植物盐胁迫抗性中的应用
CN110358772B (zh) 提高水稻非生物胁迫抗性的OsEBP89基因及制备方法与应用
CN113621625B (zh) 芝麻SiERF103基因在增强植物抗性中的应用
CN103981187B (zh) 玉米磷脂酰肌醇合成酶基因启动子P-ZmPIS的缺失突变体及其应用
CN117568305A (zh) 黄花苜蓿钙依赖蛋白激酶MfCDPK14蛋白及其应用
WO2013010368A1 (fr) Utilisation de gène clé de formation d'aérenchyme de riz oslsd2
CN114480416B (zh) 草果AtDRM2基因在提高植物耐冷性中的应用
CN112430604B (zh) 基因OsPIN10b的基因工程应用
CN112063597B (zh) 玉米多铜氧化酶编码基因ZmDEK559-2及其应用
CN114134159A (zh) 水稻基因OsWOX3B在调控根系形态中的应用
CN110760522B (zh) Ak209基因及编码蛋白与抗逆增产的应用
CN104673803B (zh) 基因甲基化在调控基因表达方面的应用
WO2024183168A1 (fr) Utilisation du gène gmsammt du soja dans la régulation de la teneur en protéines végétales et/ou du rendement
CN114958866B (zh) 调控大豆分枝数的基因及其用途
CN117625671B (zh) 基因MsFtsH11在植物抵抗非生物胁迫方面的应用
US20070028332A1 (en) Gene capable of imparting salt stress resistance
CN110734483B (zh) 耐低钾相关蛋白TaPR1及其编码基因和应用
WO2023216046A1 (fr) Gène pour réguler et contrôler le nombre de branches de soja, et son utilisation
CN116444637A (zh) 调控植物叶片衰老和产量相关蛋白GmWRKY100及其编码基因与应用

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11869528

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 11869528

Country of ref document: EP

Kind code of ref document: A1