WO2012168519A1 - Mélange de peptides ou polypeptides multifonctionnels ou combinaisons de ceux-ci pouvant former une matrice 2d ou 3d pour le soin de la peau, des muqueuses, du cuir chevelu et/ou des cheveux et leur utilisation dans des compositions cosmétiques ou dermopharmaceutiques - Google Patents

Mélange de peptides ou polypeptides multifonctionnels ou combinaisons de ceux-ci pouvant former une matrice 2d ou 3d pour le soin de la peau, des muqueuses, du cuir chevelu et/ou des cheveux et leur utilisation dans des compositions cosmétiques ou dermopharmaceutiques Download PDF

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Publication number
WO2012168519A1
WO2012168519A1 PCT/ES2012/070356 ES2012070356W WO2012168519A1 WO 2012168519 A1 WO2012168519 A1 WO 2012168519A1 ES 2012070356 W ES2012070356 W ES 2012070356W WO 2012168519 A1 WO2012168519 A1 WO 2012168519A1
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WIPO (PCT)
Prior art keywords
skin
peptides
polypeptides
forming
matrix
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PCT/ES2012/070356
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English (en)
Spanish (es)
Inventor
Luis Javier Cruz Ricondo
Carmen Gutierrez Reyes
Gerardo Alexis Acosta Crespo
Fernando Albericio Palomera
Original Assignee
Infinitec Activos, S.L.
Peptidepharma Nova, S.L.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Infinitec Activos, S.L., Peptidepharma Nova, S.L. filed Critical Infinitec Activos, S.L.
Publication of WO2012168519A1 publication Critical patent/WO2012168519A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q7/00—Preparations for affecting hair growth
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions
    • A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18

Definitions

  • compositions capable of structurally organizing and forming a 2D or 3D matrix within the skin and / or follicle providing regenerative, anti-wrinkle and / or stimulants and repair of the hair follicle.
  • composition can comprise peptides or polypeptides of this type of nature alone or in combination.
  • the present invention consists of individual multifunctional peptides and / or polypeptides and / or combinations thereof and containing biological peptide motifs, capable of structurally organizing and forming a 2D or 3D matrix within the skin giving physiological support for the regeneration and reconstruction of a new skin by sending signals to cells to activate the necessary cellular functions such as proliferation, differentiation and cell multiplication. As well as regulating all processes related to old age and hair care and maintenance. Likewise, said multifunctional peptides and / or polypeptides are applied in the treatment and care of hair. As well as its use in cosmetic and dermopharmaceutical compositions.
  • the 3 critical components for skin regeneration are i) full scaffolding with the appropriate initial biomechanical properties and the ability to sustain the complete regenerative process, ii) the appropriate molecular ligament for growth factors, functional factors, regulators and stimulants within the network can sustain cell differentiation, proliferation, and migration; iii) and finally, cells capable of responding to growth factors and all biomechanical stimuli induced by the 2D or 3D matrix.
  • the newly regenerated skin achieves rapid cellular maturity and vascular status, resident cells they must respond to biological and physical stimuli by remodeling the initial scaffolding into a functional and metabolic tissue that performs the function of the original skin.
  • the 2D or 3D extracellular matrix of the present invention retains the basement of the membrane structurally intact, collagen fibers intact, and elastin-rich microfibers; also the biomechanical components, as well as the biochemical components, such as small leucine-rich proteoglycans (decorin and biglican) necessary to sustain angiogenesis, cell migration and the global intrinsic regeneration process.
  • Cosmetic products are those products whose purpose is to provide care to the skin and hair by improving their properties, appearance and functions, as well as providing beauty and well-being to our body, mainly for the face and neck.
  • cosmetics in general, those destined for decorative cosmetics, body products or sun care are also included. Aging and other external factors can affect the functions and appearance of the skin, even altering its physiological, biochemical or histological mechanism, so that a large number of cosmetics and pharmaceuticals are used to avoid such effects.
  • the world of cosmetics which includes the development of a wide range of skin products, and therefore, helps to improve human life, is beginning a new era with technological and scientific help.
  • the skin as an organ in all its integrity, and the scalp and in a correct interaction with everything, with daily life, with stress, with the normal aging process, and hair loss and with the lesions derived from it genetically or biologically.
  • Your deep understanding is vital for the definitive solution of the problem of old age, and hair loss and care.
  • the understanding of all these intrinsic processes of repair and natural regeneration open a revolutionary path in this field of cosmetics and dermopharmaceuticals.
  • the objective of the present invention is the development of peptide assets that are capable of forming within the skin or the hair follicle a 2D or 3D matrix, combined with active fragments such as growth factors, adhesion molecules that simultaneously provide different signals to the cells for activation and stimulation. Mainly it acts on those processes that normally deteriorate and diminish over the years. Reactivate and Reversing these processes indefinitely is the goal of this innovative and revolutionary product.
  • This active peptide or polypeptide at the molecular level penetrates the skin -dermis and epidermis-root of the hair follicle- and forms a 2D or 3D network, which as a regenerating matrix, completes the process of regeneration, replacement and reconstruction of the skin and the scalp It allows to support the different cells, mainly involved in the specific functions of each application. In this way the simultaneous production of all the important proteins in the skin is reactivated, such as the different types of collagen, elastin, proteoglycans, etc. And keratin and melanin in the case of the hair follicle.
  • Another innovation of the present invention is the stabilization of these active peptides and polypeptides in their monomeric form within cosmetic or dermopharmaceutical formulations and their penetration into the skin and / or hair follicle root with subsequent spontaneous formation of the polymer network (matrix 2D or 3D) for the anchoring of cells and generation of the different activation signals thereof.
  • the 2D or 3D matrix product of the invention not only maintains a physiological support for internal regeneration in the skin and / or root of the hair follicle but also for the creation of a biomimetic matrix with biological functions that induce superficial regeneration active
  • the 2D or 3D matrix product of the invention can form autonomous mono, di and three-dimensional networks and / or in combination with those existing in the skin under natural physiological conditions, in concentrations of the order of 0.0000001% up to 100% of the weight of the structure.
  • the 2D or 3D matrix product of the invention preserves the extracellular matrix and mainly induces fibroblasts to the production of elastin, proteoglycans, laminin, tenacin, and collagen type I, III, IV, VIII. It also preserves the vascular and communication channels of the dermis and epidermis. And in the case of the hair follicle it induces proliferation, regulation of dermal porridge and stem cells which are key in the generation of new hair.
  • the 2D or 3D matrix product of the invention allows rapid revascularization and cell repopulation and maintains tension and vigor.
  • the 2D or 3D matrix product of the invention helps the recovery of discontinuities in the skin such as wrinkles, fine lines, cracks, irregularities or roughness, increased pore size, loss of elasticity, loss of firmness, loss of firmness. smoothness, loss of the ability to recover from deformation, sagging of the skin such as sagging of the cheeks, the appearance of bags under the eyes or the appearance of double chin, among others, changes in skin color such as spots, redness, dark circles , bags under the eyes or appearance of hyperpigmented areas such as age spots or freckles among others, hair loss, loss of collagen structuring and other histological changes of the stratum corneum, dermis, epidermis, vascular system.
  • peptides There are four main categories of peptides based on molecular bricks for the formation of nanostructures. 3 Among them we have i) amphiphilic peptides, ii) alkylated or acetylated peptides by large aliphatic chains, iii) phospholipopeptide conjugates and finally iv) peptide block based copolymers.
  • Amphiphilic peptides are made up of amino acids only and are organized in successions of hydrophobic and hydrophilic domains. These peptide bricks comprising two different surfaces, one hydrophilic side and the other hydrophobic allowing a certain supramolecular organization. There are different types of designs according to the distribution of positive and negative charges in the peptide sequence. 4 These types of amphiphilic peptides have been used primarily in the preparation of hydrogels. 5
  • Rapaport et al 9 investigated a family of amphiphilic peptides comprised of alternating hydrophobic and hydrophilic halves with the generic sequences of XY- (ZY) nX where the N and C-terminal (X) residues correspond to ammonium and carboxylate respectively, while Y and Z consist of alternating hydrophilic and hydrophobic amino acids.
  • Papapostolou and collaborators 12 designed a second generation of self-assembling fibers, the system was based on two complementary leucine zippers. Peptides rented with long aliphatic chains
  • Giralt et al 18 incorporated aliphatic chains in a proline-rich peptide sequence, from a family of peptides capable of penetrating at the cellular level. It was demonstrated by confocal microscopy and flow cytometry that when a tail of the myristyl group was coupled to the peptide, it significantly improved internalization in HeLa cells. On the contrary, this improvement was not observed in the case of a caproil (C6) -peptide conjugate Mono capable group studied with dioleoylphosphatidylcholine (DOPC) as a membrane model and electron transmission microscopy (TEM) demonstrates the importance of length aliphatic chain in the penetration of the cell membrane.
  • DOPC dioleoylphosphatidylcholine
  • TEM electron transmission microscopy
  • the alkylated aliphatic chain amphiphilic peptide has also been used for non-covalent functionalization of carbon nanotubes.
  • Arnold et al. 19 showed that carbon nanotubes are insoluble in water, and when they were functionalized with amphitic peptides they were soluble in solutions. aqueous.
  • solubility of functionalized carbon nanotubes could be controlled by pH adjustment.
  • Musiol et al. 20 have demonstrated the preparation of semi-synthetic lipoproteins by lipidation of the C-terminal using Huisgen copper I catalysis in the 1,3-dipolar cycloaddition.
  • 21 A 214-231 fragment of the human prion protein with a C-terminal proparglyglycine was reacted with a 1, 2-dimeristoyl-sn-glycerol-3-phosphaethanolamine (DMPE) azido-modified, mimicking the GPI anchor, to form a stable product of 1,4-disubstituted 1,3,3-triazole.
  • DMPE 2-dimeristoyl-sn-glycerol-3-phosphaethanolamine
  • the fourth class of amphiphilic peptides comprises block-based peptides of copolymers. 24, 25 Klok et al. 26 synthesized a series of hybrids and triblocks of copolymers comprising sequences of amphiphilic peptides and polyethylene glycol.
  • the copolymer blocks were synthesized by solid phase, obtaining segments of monodisperse peptides with a defined amino acid composition.
  • the self-assembly properties of the new peptide sequence were retained despite binding with PEG molecules.
  • An alternately superstructured order of PEG domains and peptide sequences with a high structural structure in beta sheet was observed. This work suggests that the combination of biological structural motifs with synthetic polymers can be a versatile strategy for the development of new self-assembling materials with complex interior structures and their potential to apply it to biology.
  • Smeenk et al. 27 described the preparation and assembly of an ABA-type triblock copolymer consisting of a block of peptide of beta core plate composed of a repetitive sequence of [(AG) 3EG] n conjugated at the end with a block of PEG.
  • EP 1419764 refers to a composition adapted to a topical application on the skin, which comprises, in a physiologically acceptable medium (i) at least one peptide or a mixture of peptides (ii) at least one inhibitor of calcium channels, where it has been shown that said association allows to neutralize the formation of wrinkles of facial expression: this association neutralizes the effects of micro-tensions on the skin, Relaxing the contractile fibroblasts of the dermis that are supposed to be involved in the genesis of expression wrinkles and thus reduce expression wrinkles and prevent them from deepening, respecting the expression of the face.
  • the sequence acetyl hexapeptide-3) hexapeptide can be used .SEC ID No. 2;
  • FIGURE 1 Study of the structures formed by the peptides of this invention.
  • the scale of the bar in all the photos was 100 um.
  • FIGURE 2 Study of cell amplification and multiplication using matrix forming peptides. The results were evaluated after 2 and 4 days of treatments with the matrix forming peptides.
  • FIGURE 3 Study by flow cytometry of the affinity of fibroblasts for matrix forming peptides. The results were evaluated after 2 and 4 days of treatments with the matrix forming peptides at different concentrations.
  • FIGURE 4 A) Collagen I expression in an untreated control of human dermal fibroblasts. B) Expression of collagen I in human dermal fibroblasts treated with a mixture of polypeptides of the invention C) Expression of collagen VI in an untreated control of human dermal fibroblasts. D) Expression of collagen VI in human dermal fibroblasts treated with the polypeptide mixture of the invention.
  • FIGURE 6 Induction of the production of type I collagen by the peptide matrix at different concentrations. Description of the invention
  • the present invention fully solves the problem of skin aging and problems related to hair loss and maintenance.
  • Applicants of the present invention have discovered that multifunctional peptides or polypeptides (hybrid peptides or signal fragments) or combinations thereof capable of spontaneously forming and / or inducing a 2D or 3D matrix within the skin and / or hair follicle contributes to the improvement, renewal and care of the skin and / or hair follicle. It involves the formation of complex matrices and superstructures that support, anchor and activate the different types of cells involved in the production of different types of collagen, elastin, glycoproteins, etc.
  • this matrix is able to form in the root of the hair serving as a support, anchor and vascular system for the dermal papilla cells and the stem cells present there. Therefore all the multifunctional peptides and polypeptides that are capable of forming a 2D or 3D matrix and / or scaffold and in combination with peptides, activation signals, regulation of specific functions and that serve as support and stimulation of the cells within the skin and / or hair follicle, providing a simple, effective and risk-free solution for the treatment and / or care of the skin, mucous membranes and / or, different types of alopecia, which includes application on the skin, mucous membranes, and / or leather scalp by different types of administration.
  • the invention relates to a multifunctional peptide or polypeptides or combinations thereof and with biological peptide motifs capable of forming a matrix, 2D or 3D, according to the following general formulas (1-9).
  • AA ⁇ AaAAs- refers to peptides or polypeptides capable of forming a 2D and 3D matrix within the skin and hair follicle
  • BB ⁇ BBaBBs- refers to peptides or polypeptides with active biological motifs capable of producing or inducing any signal or activation of interest to the skin and hair follicle, which also includes antibiotics, antimicrobials, anti-inflammatories, growth factor fragments, stimulators of Healing, cytokine fragments.
  • CC1CC2CC 3 - refers to another peptide that simultaneously with BB ⁇ BaBBs is capable of inducing or inhibiting any important activity within the skin and hair follicle, also includes fibronectin, vitronectin, polylysine, laminins, RGD fragment, polypeptides derived from the extracellular matrix.
  • R1 is independently selected from the group consisting of H, substituted or unsubstituted aliphatic group, substituted alicyclyl, substituted or unsubstituted heterocycle, substituted or unsubstituted heteroarylalkyl, substituted or unsubstituted aryl, and substituted or unsubstituted araiquyl.
  • R is selected from the group consisting of H, acetyl, tert-butanoyl, hexanoyl, hexanoyl, 2-methylhexanoyl, cyclohexanecarboxyl, can be: aliphatic compounds or not, such as octanoic acid butyric acid, decanoic acid, oleic acid, lauric acid, capric acid, myristic acid, palmitic acid, stearic acid, linoleic acid, linolenic acid, saturated or unsaturated C2-C4 alkenyls, C2-C24 substituted alkynyl, aryl or araiquyl groups
  • n is a natural number in 1, 2, 3, ... 100
  • Another aspect of the present invention is that these peptides or polypeptides may have a structure, either branched, dendrimeric, branched by the side chains, in combination with other polymers or proteins.
  • Another aspect of the present invention is directed to a cosmetic or dermopharmaceutical composition comprising an effective cosmetic or dermopharmaceutical amount of at least one peptide of general formula (1), its stereoisomers, mixtures thereof or its acceptable cosmetic or dermopharmaceutical salts and the less a cosmetic or dermopharmaceutical excipient or adjuvant for the treatment and / or care of the skin, mucous membranes, scalp and treatments for alopecia.
  • composition of the invention is composed of a percentage of the mixture of: -peptides between 0 and 100%, preferably between 0.0000001% and 20% and more preferably at a concentration between 0.0001% and 5% in weight, with respect to the total weight of the composition.
  • antioxidants including antioxidants, chelants and salts between 0 and 5%
  • the peptides of the present invention are multifunctional peptides or polypeptides (peptide hybrids), which have an important ability to spontaneously and / or induce a 2D or 3D matrix and their combination with peptides or signal fragments within the skin and / or hair follicle and therefore they are useful for the treatment of skin, mucous membranes and scalp.
  • Polypeptides and peptides are terms used interchangeably herein to designate a linear series of amino acid residues connected to each other by peptide bonds between the alpha-amino and carboxy groups of adjacent residues.
  • the polypeptide and the peptide may be a synthetic peptide or polypeptide, a recombinant peptide or recombinant polypeptide, or a peptide or polypeptide derived from enzymatic segmentation of the naturally occurring full-length protein of natural origin.
  • Synthetic peptide refers to a chain of chemically produced amino acid residues and linked together by peptide bonds, free of naturally occurring proteins and fragments thereof.
  • Multifunctional peptides or polypeptides refers to the combination of different peptides with the same and / or different activity and / or function. These peptides can be arranged in different forms: linear, branched, crosslinked
  • the peptides were synthesized using the solid phase methodology and the Fmoc / t-Bu strategy. As coupling agents for the synthesis, different combinations such as DIC / HOBt, TBTU / HOAt or HATU / HOAt were used depending on the chemical complexity of the corresponding amino acid. The elimination of the Fmoc group was performed with 20% piperidine in DMF. After synthesized, the Peptides were separated from the resin and purified by RP-HPLC. Finally, the peptides were characterized by mass spectrometry and amino acid analysis.
  • the peptides were characterized by RP-HPLC in a Water 996 photodiode detector series instrument. This instrument is equipped with a Water 2695 modular separator and a Millenium program. The reverse phase column that was used was C18 (C18 symmetry inverse phase HPLC columns, 4.6 x 150 mm, 5 ⁇ ) (Waters, Ireland). The peptides were detected at 220 nm, and a linear gradient of 5 to 100% CH3CN (+ 0.036% TFA) and H20 (+ 0.045% TFA) of 8 min was used at a flow rate of 1.0 mL / min.
  • the peptides were analyzed by MALDI-TOF and ES (+) - MS in an Applied Biosystems Voyager DE RP, using a matrix of 2.5 dihydroxybenzoic acid (DHB), and a Micromass VG-quattro spectrometer.
  • DHB 2.5 dihydroxybenzoic acid
  • the morphology of the matrix forming peptides was studied by scanning electron microscopy.
  • the matrix forming peptides were adhered to a thin glass plate. This plate was then placed on a metal plate with a double conductive tape and the peptides were coated with gold nanoparticles for 120 seconds in a vacuum system.
  • the NIH ImageJ software was used to process the photos obtained.
  • the images above show the self-assembly and structuring of the peptides of this invention, which allow to support and biological signals to the cells. And create an extracellular network and interactive communication.
  • HeLa cells were cultured in DMEM with low glucose content, containing 10% fetal bovine serum, 5% ultraglutamine, 2.5% penicillin, and 2.5% streptomycin at 37 ° C in a C0 2 controlled atmosphere incubator.
  • 96-well plates (Nalge Nunc) were previously coated with matrix-forming peptides and wells without peptides were used as experimental control.
  • HeLa cells were seeded at 1 x 10 3 cells / cm 2 in 96-well plates and cultured for 2 and 4 days. The final percentage of cell proliferation was determined with respect to the initial value of the same treated and not treated with the peptides. Results
  • This experiment demonstrates the population increase in the number of cells that is favored with the presence of the matrix forming peptides of the present invention on the plates tested. Also, the cell population was significantly increased as the days go by.
  • Human dermal fibroblasts were used to study affinity for matrix forming peptides. Thus the binding of the peptides to the fibroblast cells was studied at different concentrations (5 ug / mL, 10 ug / mL, 20 ug / mL and 40 ug / mL respectively) of the fluorescein-labeled peptide matrix for 1 h at room temperature in culture medium. Then, the human dermal fibroblasts treated with peptides and untreated were washed several times in type V plates at 1500 rpm for 2 minutes to remove peptides that did not bind to the cells. Next, the treated and untreated cells were analyzed by flow cytometry.
  • Human dermal fibroblasts were seeded at low confluence on glassware. 24 hours after sowing, they were treated with a product concentration of 0.3% of the 100ppm solution.
  • the equipment used was Scanning Electron Microscope. It is of the Field Emission Filament type. NOVA NANOSEM2 model.
  • FDH Human dermal fibroblasts
  • DMEM fetal bovine serum
  • FBS fetal bovine serum
  • the medium was replaced by fresh medium containing 0.1% FBS and the corresponding treatment to determine collagen production.
  • equal amounts of supernatant were added to a plate coated with human type I collagen antibody. The plate was incubated at room temperature for 2 hours to allow the antibody reaction and the collagen produced. Then, the plate was washed three times with PBS containing 0.5% Tween 20 to remove the remaining sample that was not bound.
  • matrix forming peptides were able to induce the activation of type I collagen.
  • Cell lines tested 1064SK (CRL-2076), human cell line; skin; morphology: fibroblasts; normal Hs68 (CRL-1635), human cell line; skin; Morphology: fibroblasts, HeLa (CCL-2), human cell line; Morphology: epithelial.
  • matrix forming peptides are not toxic. They begin to be toxic around 850 ⁇ . Therefore, these results mean that matrix forming peptides offer a wide window for cosmetic or dermopharmaceutical application.
  • compositions of the invention are capable of being incorporated into a vehicle or an acceptable cosmetic or pharmaceutical sustained release system selected from the group consisting of liposomes, millicapsules, microcapsules, nanocapsules, sponges, vesicles, micelles, microspheres, microspheres, nanospheres, lipospheres, microemulsions, nanoemulsions, miliparticles, my ero particles, nanoparticles and solid lipid nanoparticles, are also incorporated into any protein hydrolyzate whether they are of vegetable, synthetic, natural or animal origin.
  • compositions can also be found adsorbed on a solid organic polymer or cosmetically or pharmaceutically acceptable solid support.
  • a solid organic polymer or cosmetically or pharmaceutically acceptable solid support selected from the group consisting of talc, bentonite, silica, starch and maltodextrin, and dextran, and their derivatives
  • the form of presentation is selected from the group consisting of creams, multiple emulsions, anhydrous compositions, aqueous dispersions, oils, milks, balms, foams, lotions, gels, hydroalcolic solutions, liniments, serums, soaps, shampoos, ointments, mousses, ointments , powders, bars, pencils, vaporizers, aerosols, capsules, gelatin capsules, tablets, sugar-coated tablets, powders, granulated forms, chewing gums, solutions, suspensions, emulsions, syrups, jellies and gelatin.
  • a product can be incorporated is in a woven material, a non-woven fabric or a sanitary product which is selected from the group consisting of bandages, gauze, shirts, socks, socks, underwear, girdles, gloves, diapers, compresses, dressings, bedspreads, wipes, hydrogels, adhesive patches, non-adhesive patches and facial masks.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Dermatology (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Birds (AREA)
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  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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Abstract

L'invention concerne un mélange de composés de peptides et/ou polypeptides multifonctionnels individuels et/ou des combinaisons de ceux-ci et contenant des motifs peptidiques biologiques, pouvant s'organiser structurellement et former une matrice 2D ou 3D à l'intérieur de la peau et/ou du follicule, apportant des propriétés régénératives, antirides et/ou stimulantes et réparatrices du follicule pileux. L'invention concerne également son application par voie topique pour le soin de la peau et des cheveux et son utilisation dans des compositions cosmétiques ou dermopharmaceutiques, ladite composition pouvant comprendre des peptides ou polypeptides de ce type de nature, seuls ou combinés.
PCT/ES2012/070356 2011-06-09 2012-05-18 Mélange de peptides ou polypeptides multifonctionnels ou combinaisons de ceux-ci pouvant former une matrice 2d ou 3d pour le soin de la peau, des muqueuses, du cuir chevelu et/ou des cheveux et leur utilisation dans des compositions cosmétiques ou dermopharmaceutiques WO2012168519A1 (fr)

Applications Claiming Priority (2)

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ES201130969 2011-06-09
ES201130969(7) 2011-06-09

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2832742A1 (fr) 2013-08-02 2015-02-04 Infinitec Activos, S.L. Peptides pour la stimulation de la formation de collagène

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007043048A2 (fr) * 2005-10-11 2007-04-19 Ramot At Tel Aviv University Ltd. Hydrogels auto-assembles et leurs procedes d'elaboration et d'utilisation
WO2008157483A2 (fr) * 2007-06-14 2008-12-24 The Research Foundation Of State University Of New York Polypeptides et procédés d'utilisation
WO2009022339A1 (fr) * 2007-08-15 2009-02-19 Ramot At Tel Aviv University Ltd. Polypeptides, matrices, hydrogels et leurs procédés d'utilisation pour la régénération et la réparation tissulaires
US20110052693A1 (en) * 2008-02-29 2011-03-03 Bunsho Kao Method for producing artificial skin

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007043048A2 (fr) * 2005-10-11 2007-04-19 Ramot At Tel Aviv University Ltd. Hydrogels auto-assembles et leurs procedes d'elaboration et d'utilisation
WO2008157483A2 (fr) * 2007-06-14 2008-12-24 The Research Foundation Of State University Of New York Polypeptides et procédés d'utilisation
WO2009022339A1 (fr) * 2007-08-15 2009-02-19 Ramot At Tel Aviv University Ltd. Polypeptides, matrices, hydrogels et leurs procédés d'utilisation pour la régénération et la réparation tissulaires
US20110052693A1 (en) * 2008-02-29 2011-03-03 Bunsho Kao Method for producing artificial skin

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CASTELLETTO, V. ET AL.: "Fibrillar superstructure from extended nanotapes formed by a collagen-stimulating peptide", CHEM.COMM., vol. 46, no. 48, 29 October 2010 (2010-10-29), pages 9185 - 9187 *
CAVALLI, S. ET AL.: "Amphiphilic peptides and their cross-disciplinary role as building blocks for nanoscience", CHEMICAL SOCIETY REVIEWS, vol. 39, no. 1, 13 October 2009 (2009-10-13), pages 241 - 263 *
HERSEL, U. ET AL.: "RGD modified polymers: biomaterials for stimulated cell adhesion and beyond", BIOMATERIALS, vol. 24, no. 24, 1 November 2003 (2003-11-01), pages 4385 - 4415 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2832742A1 (fr) 2013-08-02 2015-02-04 Infinitec Activos, S.L. Peptides pour la stimulation de la formation de collagène
US9932368B2 (en) 2013-08-02 2018-04-03 Infinitec Activos S.L. Peptides for the stimulation of collagen formation

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