WO2012127045A1 - Cellule de levure recombinée capable de produire du gdp-fucose - Google Patents

Cellule de levure recombinée capable de produire du gdp-fucose Download PDF

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Publication number
WO2012127045A1
WO2012127045A1 PCT/EP2012/055245 EP2012055245W WO2012127045A1 WO 2012127045 A1 WO2012127045 A1 WO 2012127045A1 EP 2012055245 W EP2012055245 W EP 2012055245W WO 2012127045 A1 WO2012127045 A1 WO 2012127045A1
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WO
WIPO (PCT)
Prior art keywords
cassette
yeast
yeast cell
expression
fucose
Prior art date
Application number
PCT/EP2012/055245
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English (en)
Inventor
Christophe Javaud
Christelle ARICO
Christine BONNET
Original Assignee
Glycode
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glycode filed Critical Glycode
Priority to JP2014500415A priority Critical patent/JP2014509864A/ja
Priority to EP12710943.7A priority patent/EP2895592A1/fr
Publication of WO2012127045A1 publication Critical patent/WO2012127045A1/fr
Priority to US14/266,961 priority patent/US20140308702A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/0703—Fucose-1-phosphate guanylyltransferase (2.7.7.30)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La souche de levure ci-décrite exprime une enzyme bifonctionnelle fucokinase/ GDP-L-fucose pyrophosphorylase et est capable de produire du GDP-L-fucose in vivo. Des cellules de levure qui expriment un transporteur de GDP-L-fucose et/ou une fucosyltransférase avec l'enzyme bifonctionnelle sont également décrites. De plus, ladite levure contient une ou plusieurs cassettes d'expression pour des protéines de fusion de la voie de glycosylation hétérologue et une séquence de rétention ER/Golgi. Pour finir, cette invention concerne également un procédé de production de glycoprotéines cibles recombinées.
PCT/EP2012/055245 2011-03-23 2012-03-23 Cellule de levure recombinée capable de produire du gdp-fucose WO2012127045A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2014500415A JP2014509864A (ja) 2011-03-23 2012-03-23 Ｇｄｐ−フコースを産生可能な酵母組換え細胞
EP12710943.7A EP2895592A1 (fr) 2011-03-23 2012-03-23 Cellule de levure recombinée capable de produire du gdp-fucose
US14/266,961 US20140308702A1 (en) 2012-03-23 2014-05-01 Yeast recombinant cell capable of producing gdp-fucose

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161466639P 2011-03-23 2011-03-23
US61/466,639 2011-03-23

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US14006278 A-371-Of-International 2012-03-23
US14/266,961 Continuation US20140308702A1 (en) 2012-03-23 2014-05-01 Yeast recombinant cell capable of producing gdp-fucose

Publications (1)

Publication Number Publication Date
WO2012127045A1 true WO2012127045A1 (fr) 2012-09-27

Family

ID=45894470

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2012/055245 WO2012127045A1 (fr) 2011-03-23 2012-03-23 Cellule de levure recombinée capable de produire du gdp-fucose

Country Status (3)

Country Link
EP (1) EP2895592A1 (fr)
JP (1) JP2014509864A (fr)
WO (1) WO2012127045A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021019104A3 (fr) * 2019-09-18 2021-03-18 Basf Se Hôte de production pour la production d'oligosaccharides de lait humain

Citations (18)

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WO1995003400A1 (fr) 1993-07-23 1995-02-02 Johns Hopkins University School Of Medicine Clonage cible par recombinaison dans les chromosomes artificiels de levure
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0502298A2 (fr) 1991-01-31 1992-09-09 MERCK PATENT GmbH Procédé stéréosélectif pour la préparation de phosphates de beta-fucopyranose et de GDP-fucose très pur
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WO1995003400A1 (fr) 1993-07-23 1995-02-02 Johns Hopkins University School Of Medicine Clonage cible par recombinaison dans les chromosomes artificiels de levure
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EP1199364A2 (fr) 2000-09-26 2002-04-24 MediCel oy Utilisation d'enzymes recombinantes pour la préparation de GDP-L-fucose et de glycanes fucosylés
WO2003031464A2 (fr) 2001-10-10 2003-04-17 Neose Technologies, Inc. Remodelage et glycoconjugaison de peptides
WO2003035835A2 (fr) 2001-10-25 2003-05-01 Genentech, Inc. Compositions de glycoproteine
WO2005000862A2 (fr) 2003-06-24 2005-01-06 Isis Innovation Limited Reactifs et procedes pour former des liaisons disulfure et pour glycosyler des proteines
US20060099680A1 (en) 2004-04-23 2006-05-11 Nat Inst Of Adv Industrial Sci And Tech Yeast transformant into which genes associated with synthesis system of O-fucosylated protein are introduced
WO2006106348A2 (fr) 2005-04-08 2006-10-12 Isis Innovation Limited Glycosylation de proteines
WO2008095797A1 (fr) 2007-02-02 2008-08-14 Glycode Levures génétiquement modifiées pour la production de glycoprotéines homogènes
WO2008112092A2 (fr) 2007-03-07 2008-09-18 Glycofi, Inc. Production de glycoprotéines à fucosylation modifiée
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021019104A3 (fr) * 2019-09-18 2021-03-18 Basf Se Hôte de production pour la production d'oligosaccharides de lait humain

Also Published As

Publication number Publication date
EP2895592A1 (fr) 2015-07-22
JP2014509864A (ja) 2014-04-24

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