WO2012081931A3 - 세포배양을 통한 세포 및 지용성물질의 생산 방법 및 장치 - Google Patents
세포배양을 통한 세포 및 지용성물질의 생산 방법 및 장치 Download PDFInfo
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- WO2012081931A3 WO2012081931A3 PCT/KR2011/009716 KR2011009716W WO2012081931A3 WO 2012081931 A3 WO2012081931 A3 WO 2012081931A3 KR 2011009716 W KR2011009716 W KR 2011009716W WO 2012081931 A3 WO2012081931 A3 WO 2012081931A3
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- fat soluble
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
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- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
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- C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
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- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
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- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/16—Butanols
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- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/54—Acetic acid
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- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
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- C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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- Tropical Medicine & Parasitology (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
본 발명은 세포배양용액으로부터 손상 없는 세포와 지용성물질을 저비용 고효율로 생산하는 방법 및 장치에 관한 것이다. 본 발명에 따른 세포배양을 통한 세포 및 지용성물질의 생산 장치는, 지용성물질을 함유하는 세포를 배양하는 배양장치(10); 상기 세포의 상기 지용성물질이 용해되는 지용성물질추출용매를 저장 및 공급하는 용매장치(20); 상기 배양장치(10)로부터의 상기 세포배양용액과 상기 용매장치(20)로부터의 상기 지용성물질추출용매를 혼합하는 혼합장치(30); 상기 혼합장치(30)의 혼합용액에서 상기 세포를 분리하는 분리장치(40); 상기 세포가 분리된 상기 분리장치(40)로부터의 용액을, 상기 지용성물질추출용매에 상기 세포배양용액의 상기 지용성물질이 용해된 지용성물질-용매와, 물로 분획하는 분획장치(50); 상기 분리장치(40)로부터 분리된 상기 세포를 수용 또는 처리하는 세포수용장치(60); 및 상기 분획장치(50)로부터 분획한 상기 지용성물질-용매를 수용 또는 처리하는 지용성물질용매수용장치(70); 를 포함한다.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US13/995,068 US20130309757A1 (en) | 2010-12-17 | 2011-12-16 | Method and apparatus for producing cells and fat soluble materials by cell culture |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR20100129852A KR101194942B1 (ko) | 2010-12-17 | 2010-12-17 | 유기성폐기물의 미세조류배양에 의한 바이오가스, 지용성물질 및 미세조류의 생산 방법 및 장치 |
KR10-2010-0129852 | 2010-12-17 | ||
KR10-2011-0074014 | 2011-07-26 | ||
KR20110074014 | 2011-07-26 |
Publications (2)
Publication Number | Publication Date |
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WO2012081931A2 WO2012081931A2 (ko) | 2012-06-21 |
WO2012081931A3 true WO2012081931A3 (ko) | 2012-10-11 |
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PCT/KR2011/009716 WO2012081931A2 (ko) | 2010-12-17 | 2011-12-16 | 세포배양을 통한 세포 및 지용성물질의 생산 방법 및 장치 |
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US (1) | US20130309757A1 (ko) |
WO (1) | WO2012081931A2 (ko) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
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US10704021B2 (en) | 2012-03-15 | 2020-07-07 | Flodesign Sonics, Inc. | Acoustic perfusion devices |
TWI637057B (zh) * | 2012-11-09 | 2018-10-01 | 拜爾沙納有限公司 | 具交替生物反應器用途之不連續進料批次製程 |
CA2916609C (en) * | 2013-06-27 | 2022-05-03 | Sasol Technology (Proprietary) Limited | Production of biomass for use in the treatment of fischer-tropsch reaction water |
CA2935960C (en) | 2014-01-08 | 2023-01-10 | Bart Lipkens | Acoustophoresis device with dual acoustophoretic chamber |
US20150210979A1 (en) * | 2014-01-27 | 2015-07-30 | Northrop Grumman Systems Corporation | Scaffold-free tissue engineering using field induced forces |
EP3130660A4 (en) * | 2014-03-25 | 2017-11-08 | Tokyo Electron Limited | Cell separation device and cell separation method |
US11708572B2 (en) | 2015-04-29 | 2023-07-25 | Flodesign Sonics, Inc. | Acoustic cell separation techniques and processes |
US11377651B2 (en) | 2016-10-19 | 2022-07-05 | Flodesign Sonics, Inc. | Cell therapy processes utilizing acoustophoresis |
EP3288660A1 (en) * | 2015-04-29 | 2018-03-07 | Flodesign Sonics Inc. | Acoustophoretic device for angled wave particle deflection |
US11214789B2 (en) | 2016-05-03 | 2022-01-04 | Flodesign Sonics, Inc. | Concentration and washing of particles with acoustics |
SG11202003907WA (en) | 2017-12-14 | 2020-05-28 | Flodesign Sonics Inc | Acoustic transducer drive and controller |
CN111630146A (zh) | 2018-01-09 | 2020-09-04 | 弗洛设计声能学公司 | 用于细胞疗法和基因疗法的声学加工 |
EP3762482A4 (en) * | 2018-03-08 | 2022-01-05 | FloDesign Sonics, Inc. | CELL THERAPY METHOD USING ACUSTOPHORESIS |
DE102018211281A1 (de) * | 2018-07-09 | 2020-01-09 | Robert Bosch Gmbh | Mikrofluidische Vorrichtung und Verfahren zum Verdünnen und Separieren von Partikeln einer Probe |
CN108949523B (zh) * | 2018-09-19 | 2023-07-07 | 青岛中科蓝智生物科技发展有限公司 | 一种工业化生产甘油葡萄糖苷的工艺及系统 |
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KR20060097947A (ko) * | 2005-03-08 | 2006-09-18 | 진현진 | 클로렐라로부터 액상 추출물을 추출하는 방법 |
US20080155888A1 (en) * | 2006-11-13 | 2008-07-03 | Bertrand Vick | Methods and compositions for production and purification of biofuel from plants and microalgae |
KR100949314B1 (ko) * | 2009-08-28 | 2010-03-23 | 한밭대학교 산학협력단 | 유기성폐기물의 혐기성 소화액을 이용한 조류생산 장치 |
KR100983023B1 (ko) * | 2010-07-07 | 2010-09-17 | 한국해양연구원 | 부등편모조류 또는 착편모조류에 속하는 미세조류의 지방산으로부터 트리글리세라이드 또는 지방산메틸에스테르를 추출하는 방법 및 이를 이용한 바이오디젤 제조방법 |
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US20090104157A1 (en) * | 2007-10-05 | 2009-04-23 | E. I. Du Pont De Nemours And Company | Utilization of bacteriophage to control bacterial contamination in fermentation processes |
MX2010006168A (es) * | 2007-12-04 | 2010-09-22 | Univ Ohio State Res Found | Optimizacion de la produccion de biocombustibles. |
US20120095245A1 (en) * | 2009-05-11 | 2012-04-19 | Phycal, Inc. | Biofuel production from algae |
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2011
- 2011-12-16 WO PCT/KR2011/009716 patent/WO2012081931A2/ko active Application Filing
- 2011-12-16 US US13/995,068 patent/US20130309757A1/en not_active Abandoned
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20060097947A (ko) * | 2005-03-08 | 2006-09-18 | 진현진 | 클로렐라로부터 액상 추출물을 추출하는 방법 |
US20080155888A1 (en) * | 2006-11-13 | 2008-07-03 | Bertrand Vick | Methods and compositions for production and purification of biofuel from plants and microalgae |
KR100949314B1 (ko) * | 2009-08-28 | 2010-03-23 | 한밭대학교 산학협력단 | 유기성폐기물의 혐기성 소화액을 이용한 조류생산 장치 |
KR100983023B1 (ko) * | 2010-07-07 | 2010-09-17 | 한국해양연구원 | 부등편모조류 또는 착편모조류에 속하는 미세조류의 지방산으로부터 트리글리세라이드 또는 지방산메틸에스테르를 추출하는 방법 및 이를 이용한 바이오디젤 제조방법 |
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WO2012081931A2 (ko) | 2012-06-21 |
US20130309757A1 (en) | 2013-11-21 |
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