WO2012070795A3 - Mutation du gène ibor-ins issu de ipomoea batatas et son utilisation - Google Patents

Mutation du gène ibor-ins issu de ipomoea batatas et son utilisation Download PDF

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Publication number
WO2012070795A3
WO2012070795A3 PCT/KR2011/008671 KR2011008671W WO2012070795A3 WO 2012070795 A3 WO2012070795 A3 WO 2012070795A3 KR 2011008671 W KR2011008671 W KR 2011008671W WO 2012070795 A3 WO2012070795 A3 WO 2012070795A3
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WO
WIPO (PCT)
Prior art keywords
derived
ipomoea batatas
ibor
gene mutation
transformed plant
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PCT/KR2011/008671
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English (en)
Korean (ko)
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WO2012070795A2 (fr
WO2012070795A9 (fr
Inventor
곽상수
이행순
안영옥
정재철
김선하
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한국생명공학연구원
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Priority to CN2011800561532A priority Critical patent/CN103269578A/zh
Publication of WO2012070795A2 publication Critical patent/WO2012070795A2/fr
Publication of WO2012070795A3 publication Critical patent/WO2012070795A3/fr
Publication of WO2012070795A9 publication Critical patent/WO2012070795A9/fr

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un procédé de production : d'une mutation du gène IbOr-Ins dans lequel une certaine séquence est artificiellement insérée à l'intérieur d'un gène IbOrange issu de Ipomoea batatas ; d'un vecteur recombinant comprenant la mutation génique; d'une cellule hôte mutée par le vecteur recombinant; et d'un corps végétal transformé qui a une teneur en caroténoïdes et une résistance au stress salin améliorées au moyen de la mutation du vecteur recombinant dans une cellule végétale. La présente invention concerne également un procédé de production d'un corps végétal transformé ayant une résistance accrue au stress salin à l'aide du corps végétal transformé ou de ses graines produit(es) par le procédé mentionné ci-dessus, et ayant une teneur en caroténoïde et une résistance au stress salin améliorées, et l'utilisation d'un gène IbOrange issu de Ipomoea batatas. L'invention concerne également un corps végétal transformé produit par le procédé mentionné ci-dessus et ayant une résistance accrue au stress salin, et ses graines.
PCT/KR2011/008671 2010-11-22 2011-11-14 Mutation du gène ibor-ins issu de ipomoea batatas et son utilisation WO2012070795A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2011800561532A CN103269578A (zh) 2010-11-22 2011-11-14 源自甘薯的IbOr-Ins基因突变体及其用途

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020100115965A KR101281071B1 (ko) 2010-11-22 2010-11-22 고구마 유래의 ＩｂＯｒ-Ｉｎｓ 유전자 변이체 및 이의 용도
KR10-2010-0115965 2010-11-22

Publications (3)

Publication Number Publication Date
WO2012070795A2 WO2012070795A2 (fr) 2012-05-31
WO2012070795A3 true WO2012070795A3 (fr) 2012-10-11
WO2012070795A9 WO2012070795A9 (fr) 2012-11-08

Family

ID=46146247

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/008671 WO2012070795A2 (fr) 2010-11-22 2011-11-14 Mutation du gène ibor-ins issu de ipomoea batatas et son utilisation

Country Status (3)

Country Link
KR (1) KR101281071B1 (fr)
CN (1) CN103269578A (fr)
WO (1) WO2012070795A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101359308B1 (ko) * 2011-08-05 2014-02-10 한국생명공학연구원 카로티노이드 및 안토시아닌 고축적 형질전환 고구마 식물체의 제조 방법 및 그에 따른 식물체
KR101478112B1 (ko) * 2012-10-12 2014-12-31 한국생명공학연구원 흑반병 저항성을 증가시키는 고구마 ＩｂＯｒ-Ｉｎｓ 유전자 및 이의 용도
KR102110870B1 (ko) * 2017-12-19 2020-05-15 한국생명공학연구원 고구마 유래의 IbOr-R96H 변이체 및 이의 용도
CN108834803A (zh) * 2018-07-12 2018-11-20 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) 一种诱导甘薯现蕾的方法
KR102525540B1 (ko) * 2021-03-05 2023-04-25 한국생명공학연구원 당근 유래 lcyb2 단백질 변이체 및 이의 용도
CN117164686B (zh) * 2022-05-11 2024-05-31 中国农业大学 抗逆相关蛋白IbRCD1及其相关生物材料与应用

Citations (1)

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KR100990330B1 (ko) * 2008-06-20 2010-10-29 한국생명공학연구원 고구마 유래의 카로티노이드 축적에 관련된ＩｂＯｒａｎｇｅ 유전자

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US8138391B1 (en) 2005-12-07 2012-03-20 The United States Of America As Represented By The Secretary Of Agriculture Or gene and its use in manipulating carotenoid content and composition in plants and other organisms
KR100813284B1 (ko) 2006-04-28 2008-03-13 제주대학교 산학협력단 감귤 피토엔 생합성 효소 ｐｓｙ 유전자가 형질전환된유채식물체
KR20100100097A (ko) * 2009-03-05 2010-09-15 화이젠 주식회사 고구마 유래의 ＭｕＳ1 유전자 및 이의 용도

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KR100990330B1 (ko) * 2008-06-20 2010-10-29 한국생명공학연구원 고구마 유래의 카로티노이드 축적에 관련된ＩｂＯｒａｎｇｅ 유전자

Non-Patent Citations (4)

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HIRONORI MANO ET AL.: "Isolation of a Regulatory Gene of Anthocyanin Biosynthesis in Tuberous Roots of Purple-Fleshed Sweet Potato", PLANT PHYSIOLOGY, vol. 143, 2007, pages 1252 - 1268, XP003019791, DOI: doi:10.1104/pp.106.094425 *
MYOUNG DUCK KIM ET AL.: "Selection of transgenic sweetpotato plants expressing 2-Cys peroxiredoxin with enhanced tolerance to oxidative stress", JOURNAL OF BIOTECHNOLOGY, vol. 36, no. 1, 2009, pages 75 - 80 *
SALGADO-GARCIGLIA ET AL.: "NaCl-resistant variant cells isolated from sweet potato cell suspensions", PLANT CELL TISSUE ORGAN CULTURE, vol. 5, 1985, pages 3 - 12 *
SHAN LU ET AL.: "The Cauliflower Or Gene Encodes a DnaJ Cysteine-Rich Doma in-Containing Protein That Mediates High Levels of b-Carotene Accumulation", THE PLANT CELL, vol. 18, 2006, pages 3594 - 3605 *

Also Published As

Publication number Publication date
CN103269578A (zh) 2013-08-28
KR20120054712A (ko) 2012-05-31
KR101281071B1 (ko) 2013-07-09
WO2012070795A2 (fr) 2012-05-31
WO2012070795A9 (fr) 2012-11-08

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