WO2012050425A2 - A process for extracting antioxidants from oil palm biomass - Google Patents

A process for extracting antioxidants from oil palm biomass Download PDF

Info

Publication number
WO2012050425A2
WO2012050425A2 PCT/MY2011/000217 MY2011000217W WO2012050425A2 WO 2012050425 A2 WO2012050425 A2 WO 2012050425A2 MY 2011000217 W MY2011000217 W MY 2011000217W WO 2012050425 A2 WO2012050425 A2 WO 2012050425A2
Authority
WO
WIPO (PCT)
Prior art keywords
oil palm
vessel
biomass
water soluble
solvent
Prior art date
Application number
PCT/MY2011/000217
Other languages
French (fr)
Other versions
WO2012050425A3 (en
Inventor
Mei Han Ng
Yuen May Choo
Original Assignee
Malasian Palm Oil Board
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Malasian Palm Oil Board filed Critical Malasian Palm Oil Board
Priority to SG2013027313A priority Critical patent/SG189364A1/en
Publication of WO2012050425A2 publication Critical patent/WO2012050425A2/en
Publication of WO2012050425A3 publication Critical patent/WO2012050425A3/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/889—Arecaceae, Palmae or Palmaceae (Palm family), e.g. date or coconut palm or palmetto
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/02—Pretreatment
    • C11B1/04—Pretreatment of vegetable raw material
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/10—Production of fats or fatty oils from raw materials by extracting
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/003—Refining fats or fatty oils by enzymes or microorganisms, living or dead
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/10—Refining fats or fatty oils by adsorption
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction

Definitions

  • the present invention relates to a process for extracting compounds with anti- oxidative properties from oil palm biomass such as oil palm leaves, palm pressed fibre and empty frui bunches.
  • the oil palm produces a vast variety of biomass, i.e. frond, empty fruit bunches, pressed fibre, etc.
  • biomass i.e. frond, empty fruit bunches, pressed fibre, etc.
  • Each of these types of biomass has applications in different industries such as pulp and paper, wood and bio-energy industries.
  • the oil palm biomass is a potential source of water soluble antioxidants. Concentration of total phenolic compounds, a type of water soluble antioxidants in oil palm leaves, pressed fibre and empty fruit bunches ranges from 5 to 20mg/g of biomass. Therefore there is a need to extract the water soluble antioxidants from the oil palm biomass.
  • the present invention relates to a process for extracting and recovering of water soluble compounds with anti-oxidative properties from oil palm biomass such as oil palm leaves, palm pressed fibre and empty palm fruit bunches.
  • oil palm biomass such as oil palm leaves, palm pressed fibre and empty palm fruit bunches.
  • the second embodiment of the process for extracting water soluble compounds with anti-oxidative properties from oil palm biomass includes the steps of:
  • the process for extracting water soluble compounds with anti-oxidative properties from oil palm biomass can be a sub critical water extraction, sub critical carbon dioxide extraction, supercritical water extraction or supercritical carbon dioxide extraction.
  • the vessel is heated at a temperature range of 30-75°C upon loading of the oil palm biomass in the vessel in all the four embodiments.
  • the adsorbent is silica gel.
  • the solvent is alcohol such as methanol. DETAILED DESCRIPTION OF THE INVENTION
  • the present invention relates to a process for extracting and recovering of water soluble compounds with anti-oxidative properties from oil palm biomass such as oil palm leaves, palm pressed fibre and empty palm fruit bunches.
  • oil palm biomass such as oil palm leaves, palm pressed fibre and empty palm fruit bunches.
  • the four different embodiments are as follows:
  • the adsorbent is silica gel and the solvent is alcohol such as methanol.
  • All the four embodiments require grinding of the palm biomass into smaller mass or sizes prior to the process for extracting and recovering of water soluble compounds. This allows for higher extraction efficiency. Once the oil palm biomass is grinded into smaller sizes, they are ready for extraction.
  • the first embodiment relates to a process for extracting water soluble compounds with anti-oxidative properties without solvent and without adsorbent.
  • the oil palm biomass is loaded into an empty vessel. Then, the vessel is sealed.
  • the vessel comprises a vent for gas to pass through at top of the vessel and another vent or outlet at the other end. Gas which is pressurized and heated to certain pressure and temperature (termed as super or sub critical state) is pumped into the vessel.
  • the gas which is passing through the oil palm biomass dissolves and carries the anti-oxidative compounds along as it is being released from the outlet of the vessel.
  • the gas released from the outlet is then collected. Once the gas is released from the vessel, it depressurized spontaneously, leaving behind the anti-oxidative compounds.
  • the second embodiment is similar to the first embodiment with the exception that the oil palm biomass is first immersed in a solvent and the solvent is then concentrated to a minimal volume. The solvent is then loaded into a vessel pre-packed with an adsorbent. Pressurized gas is then pumped into the vessel and the anti-oxidative compounds are being carried through the adsorbent to an outlet of the vessel upon which, they are collected.
  • This embodiment gives an advantage of being able to concentrate the anti- oxidative compounds in the oil palm biomass prior to extraction, thus is more efficient and gives higher throughput.
  • the third embodiment is similar to the second whereby prior to extraction, the oil palm biomass is pre concentrated in a solvent and the solvent is loaded into a vessel pre packed with adsorbent.
  • a solvent is pumped and pre mixed with pressurized gas before they are pumped into the vessel.
  • the pressurized gas, solvent and anti-oxidative compounds from the oil palm biomass are then collected at an outlet of the vessel.
  • This embodiment involves a solvent and therefore yields higher extraction efficiency than the embodiment without the presence of a solvent.
  • the fourth embodiment is similar to the first embodiment whereby the oil palm biomass is loaded straight into an empty vessel without the need for pre concentration in a solvent. A solvent is pumped and pre mixed with pressurized gas before they are pumped into the vessel.
  • the pressurized gas, solvent and anti-oxidative compounds from the oil palm biomass are then collected at an outlet of the vessel.
  • This embodiment involves a solvent and therefore yields higher efficiency than the embodiment without the presence of solvent.
  • the pressurized gas is carbon dioxide having pressure range of 30-70MPa.
  • the vessel is heated at a temperature range of 30-75°C upon loading of the oil palm biomass in the vessel in all the four embodiments.
  • the adsorbent is silica gel.
  • the solvent is alcohol such as methanol.
  • EXAMPLE 1 Empty fruit bunch that is obtained upon pressing of oil palm fruit bunches for its oil is ground into powder form of 2 - 10mm length. 30g of the grounded empty fruit bunch are loaded into an empty, 30mL extraction vessel. The extraction vessel is then heated to 40°C. Pressurized carbon dioxide (150 bars, - 4°C) is pumped into the vessel for 3 hours. The extract containing the anti-oxidative compounds that come out from an outlet of the extraction vessel is collected.
  • Fresh oil palm leaves are grounded into powder form of 2 - 7mm length. 30g of the grounded oil palm leaves is loaded into an empty, 30mL extraction vessel. The extraction vessel is then heated to 40°C. Pressurized carbon dioxide (150 bars, - 4°C) is pumped into the vessel for 3 hours. The extract containing the anti-oxidative compounds that come out from an outlet of the extraction vessel is collected.
  • Fresh oil palm leaves are grounded into powder form of 2 - 10mm length. 5g of the grounded oil palm leaves is- immersed in 30mL methanol and mixed vigorously. Thereafter, the mixture is filtered and the methanol in the filtrate is removed till only about 5mL remained. lmL of the filtrate is loaded into 250mm length x 200mm internal diameter vessel which is pre-packed with silica gel. The extraction vessel is then heated to 40°C. Pressurized carbon dioxide (150 bars, - 4°C) is pumped into the vessel for lhour. The extract containing the anti-oxidative compounds that come out from an outlet of the extraction vessel is collected.
  • Fresh oil palm leaves are grounded into powder form of 2 - 10mm length. 5g of the grounded empty fruit bunch is immersed in 30mL methanol and mixed vigorously. Thereafter, the mixture is filtered and the methanol in the filtrate is removed till only about 5mL remained. lmL of the filtrate is loaded into 250mm length x 200mm internal diameter vessel which is pre-packed with silica gel. The extraction vessel is then heated to 40°C. Pressurized carbon dioxide (150 bars, - 4°C) and 0.5mL methanol are pumped into the vessel simultaneously for 1 hour. The extract containing the anti-oxidative compounds that come out from an outlet of the extraction vessel is collected.
  • Fresh oil palm leaves are grounded into powder form of 2 - 10mm length. 30g of the grounded oil palm leaves is loaded into an empty, 30mL extraction vessel. The extraction vessel is then heated to 40°C. Pressurized carbon dioxide (150 bars, - 4°C) and 0.2mL methanol are pumped into the vessel simultaneously for 3 hours. The extract containing the anti-oxidative compounds that come out from an outlet of the extraction vessel is collected.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Wood Science & Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biotechnology (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Medical Informatics (AREA)
  • Botany (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Toxicology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Fats And Perfumes (AREA)

Abstract

This present invention relates to a process for extracting and recovering of water soluble compounds with anti-oxidative properties from oil palm biomass such as oil palm leaves, palm pressed fibre and empty fruit bunches. The process can be a sub critical water extraction, sub critical carbon dioxide extraction, supercritical water extraction or supercritical carbon dioxide extraction. Concentration of total phenolic compounds, a type of water soluble antioxidants in the oil palm leaves, pressed fibre and empty fruit bunches ranges from 5 to 20mg/g of biomass. These water soluble antioxidants can be extracted and recovered by way of solvent free process which is more favorable as the usage of organic solvents during extraction may pose problems of toxicity, waste disposal, storage as well as health issues. There are four different embodiments for extracting water soluble compounds with anti-oxidative properties. The four different embodiments are as follows: i) a process without solvent and without adsorbent; ii) a process without solvent but with adsorbent; iii) a process with solvent and with adsorbent; and iv) a process with solvent but without adsorbent. The adsorbent is silica gel and the solvent is alcohol such as methanol.

Description

A PROCESS FOR EXTRACTING ANTIOXIDANTS FROM OIL PALM
BIOMASS
FIELD OF INVENTION
The present invention relates to a process for extracting compounds with anti- oxidative properties from oil palm biomass such as oil palm leaves, palm pressed fibre and empty frui bunches.
BACKGROUND OF THE INVENTION
The oil palm produces a vast variety of biomass, i.e. frond, empty fruit bunches, pressed fibre, etc. Each of these types of biomass has applications in different industries such as pulp and paper, wood and bio-energy industries. Before being treated and used for these applications, the oil palm biomass is a potential source of water soluble antioxidants. Concentration of total phenolic compounds, a type of water soluble antioxidants in oil palm leaves, pressed fibre and empty fruit bunches ranges from 5 to 20mg/g of biomass. Therefore there is a need to extract the water soluble antioxidants from the oil palm biomass.
SUMMARY OF THE INVENTION
The present invention relates to a process for extracting and recovering of water soluble compounds with anti-oxidative properties from oil palm biomass such as oil palm leaves, palm pressed fibre and empty palm fruit bunches. There are four different embodiments for extracting water soluble compounds with anti-oxidative properties.
The first embodiment of the process for extracting water soluble compounds with anti-oxidative properties from oil palm biomass includes the steps of:
i) reducing size of the oil palm biomass between 2- 10mm length;
ii) loading the oil palm biomass from step (i) into a vessel;
iii) subjecting the oil palm biomass with a stream of carbon dioxide having pressure range of 30-70MPa;
iv) collecting water soluble compounds released from an outlet of the vessel. The second embodiment of the process for extracting water soluble compounds with anti-oxidative properties from oil palm biomass includes the steps of:
i) reducing size of the oil palm biomass between 2- 10mm length;
ii) immersing the oil palm biomass in a solvent;
iii) loading the oil palm biomass from step (ii) into a vessel containing adsorbent;
iv) subjecting the oil palm biomass with a stream of carbon dioxide having pressure range of 30-70MPa ;
v) collecting water soluble compounds released from an outlet of the vessel.
The third embodiment of the process for extracting water soluble compounds with anti-oxidative properties from oil palm biomass includes the steps of:
i) reducing size of the oil palm biomass between 2- 10mm length;
ii) immersing the oil palm biomass in a solvent;
iii) loading the oil palm biomass from step (ii) into a vessel containing adsorbent;
iv) subjecting the oil palm biomass with a stream of carbon dioxide having pressure range of 30-70MPa and a stream of solvent simultaneously;
v) collecting water soluble compounds released from an outlet of the vessel.
The fourth embodiment of the process for extracting water soluble compounds with anti-oxidative properties from oil palm biomass includes the steps of:
i) reducing size of the oil palm biomass between 2- 10mm length;
ii) loading the oil palm biomass from step (i) into a vessel;
iii) subjecting the oil palm biomass with a stream of carbon dioxide having pressure range of 30-70MPa and a stream of solvent simultaneously;
iv) collecting water soluble compounds released from an outlet of the vessel.
The process for extracting water soluble compounds with anti-oxidative properties from oil palm biomass can be a sub critical water extraction, sub critical carbon dioxide extraction, supercritical water extraction or supercritical carbon dioxide extraction. The vessel is heated at a temperature range of 30-75°C upon loading of the oil palm biomass in the vessel in all the four embodiments. The adsorbent is silica gel. The solvent is alcohol such as methanol. DETAILED DESCRIPTION OF THE INVENTION
The present invention relates to a process for extracting and recovering of water soluble compounds with anti-oxidative properties from oil palm biomass such as oil palm leaves, palm pressed fibre and empty palm fruit bunches. There are four different embodiments for extracting water soluble compounds with anti-oxidative properties. The four different embodiments are as follows:
i) a process without solvent and without adsorbent;
ii) a process without solvent but with adsorbent;
iii) a process with solvent and with adsorbent; and
iv) a process with solvent but without adsorbent.
The adsorbent is silica gel and the solvent is alcohol such as methanol.
Hereinafter, this specification will describe the present invention according to the preferred embodiments of the present invention. However, it is to be understood that limiting the description to the preferred embodiments of the invention is merely to facilitate discussion of the present invention and it is envisioned that those skilled in the art may devise various modifications and equivalents without departing from the scope of the appended claims.
All the four embodiments require grinding of the palm biomass into smaller mass or sizes prior to the process for extracting and recovering of water soluble compounds. This allows for higher extraction efficiency. Once the oil palm biomass is grinded into smaller sizes, they are ready for extraction.
The first embodiment relates to a process for extracting water soluble compounds with anti-oxidative properties without solvent and without adsorbent. In this case, the oil palm biomass is loaded into an empty vessel. Then, the vessel is sealed. The vessel comprises a vent for gas to pass through at top of the vessel and another vent or outlet at the other end. Gas which is pressurized and heated to certain pressure and temperature (termed as super or sub critical state) is pumped into the vessel. The gas which is passing through the oil palm biomass, dissolves and carries the anti-oxidative compounds along as it is being released from the outlet of the vessel. The gas released from the outlet is then collected. Once the gas is released from the vessel, it depressurized spontaneously, leaving behind the anti-oxidative compounds.
The second embodiment is similar to the first embodiment with the exception that the oil palm biomass is first immersed in a solvent and the solvent is then concentrated to a minimal volume. The solvent is then loaded into a vessel pre-packed with an adsorbent. Pressurized gas is then pumped into the vessel and the anti-oxidative compounds are being carried through the adsorbent to an outlet of the vessel upon which, they are collected. This embodiment gives an advantage of being able to concentrate the anti- oxidative compounds in the oil palm biomass prior to extraction, thus is more efficient and gives higher throughput.
The third embodiment is similar to the second whereby prior to extraction, the oil palm biomass is pre concentrated in a solvent and the solvent is loaded into a vessel pre packed with adsorbent. In this embodiment however, a solvent is pumped and pre mixed with pressurized gas before they are pumped into the vessel. The pressurized gas, solvent and anti-oxidative compounds from the oil palm biomass are then collected at an outlet of the vessel. This embodiment involves a solvent and therefore yields higher extraction efficiency than the embodiment without the presence of a solvent. The fourth embodiment is similar to the first embodiment whereby the oil palm biomass is loaded straight into an empty vessel without the need for pre concentration in a solvent. A solvent is pumped and pre mixed with pressurized gas before they are pumped into the vessel. The pressurized gas, solvent and anti-oxidative compounds from the oil palm biomass are then collected at an outlet of the vessel. This embodiment involves a solvent and therefore yields higher efficiency than the embodiment without the presence of solvent. In all the four embodiments, the pressurized gas is carbon dioxide having pressure range of 30-70MPa. The vessel is heated at a temperature range of 30-75°C upon loading of the oil palm biomass in the vessel in all the four embodiments. The adsorbent is silica gel. The solvent is alcohol such as methanol.
The invention will be further understood from the following non-limited examples. EXAMPLE 1 Empty fruit bunch that is obtained upon pressing of oil palm fruit bunches for its oil is ground into powder form of 2 - 10mm length. 30g of the grounded empty fruit bunch are loaded into an empty, 30mL extraction vessel. The extraction vessel is then heated to 40°C. Pressurized carbon dioxide (150 bars, - 4°C) is pumped into the vessel for 3 hours. The extract containing the anti-oxidative compounds that come out from an outlet of the extraction vessel is collected.
EXAMPLE 2
Empty fruit bunch that is obtained upon pressing of oil palm fruit bunches for its oil is ground into powder form of 2 - 10mm length. 5g of the grounded empty fruit bunch is immersed in 30mL methanol and mixed vigorously. Thereafter, the mixture is filtered and the methanol in the filtrate is removed till only about 5mL remained. lmL of the filtrate is loaded into 250mm length x 200mm internal diameter vessel which is prepacked with silica gel. The extraction vessel is then heated to 40°C. Pressurized carbon dioxide (150 bars, - 4°C) is pumped into the vessel for lhour. The extract containing the anti-oxidative compounds that come out from an outlet of the extraction vessel is collected.
EXAMPLE 3
Empty fruit bunch that is obtained upon pressing of oil palm fruit bunches for its oil is ground into powder form of 2 - 10mm length. 5g of the grounded empty fruit bunch is immersed in 30mL methanol and mixed vigorously. Thereafter, the mixture is filtered and the methanol in the filtrate is removed till only about 5mL remained. lmL of the filtrate is loaded into 250mm length x 200mm internal diameter vessel which is prepacked with silica gel. The extraction vessel is then heated to 40°C. Pressurized carbon dioxide (150 bars, - 4°C) and 0.5mL methanol are pumped into the vessel simultaneously for 1 hour. The extract containing the anti-oxidative compounds that come out from an outlet of the extraction vessel is collected.
EXAMPLE 4
Empty fruit bunch that is obtained upon pressing of oil palm fruit bunches for its oil is ground into powder form of 2 - 10mm length. 30g of the grounded empty fruit bunch was loaded into an empty, 30mL extraction vessel. The extraction vessel is then heated to 40°C. Pressurized carbon dioxide (150 bars, - 4°C) and 0.2mL methanol are pumped into the vessel simultaneously for 3 hours. The extract containing the anti-oxidative compounds that come out from an outlet of the extraction vessel is collected.
EXAMPLE 5 Fresh oil palm leaves are grounded into powder form of 2 - 7mm length. 30g of the grounded oil palm leaves is loaded into an empty, 30mL extraction vessel. The extraction vessel is then heated to 40°C. Pressurized carbon dioxide (150 bars, - 4°C) is pumped into the vessel for 3 hours. The extract containing the anti-oxidative compounds that come out from an outlet of the extraction vessel is collected.
EXAMPLE 6
Fresh oil palm leaves are grounded into powder form of 2 - 10mm length. 5g of the grounded oil palm leaves is- immersed in 30mL methanol and mixed vigorously. Thereafter, the mixture is filtered and the methanol in the filtrate is removed till only about 5mL remained. lmL of the filtrate is loaded into 250mm length x 200mm internal diameter vessel which is pre-packed with silica gel. The extraction vessel is then heated to 40°C. Pressurized carbon dioxide (150 bars, - 4°C) is pumped into the vessel for lhour. The extract containing the anti-oxidative compounds that come out from an outlet of the extraction vessel is collected.
EXAMPLE 7
Fresh oil palm leaves are grounded into powder form of 2 - 10mm length. 5g of the grounded empty fruit bunch is immersed in 30mL methanol and mixed vigorously. Thereafter, the mixture is filtered and the methanol in the filtrate is removed till only about 5mL remained. lmL of the filtrate is loaded into 250mm length x 200mm internal diameter vessel which is pre-packed with silica gel. The extraction vessel is then heated to 40°C. Pressurized carbon dioxide (150 bars, - 4°C) and 0.5mL methanol are pumped into the vessel simultaneously for 1 hour. The extract containing the anti-oxidative compounds that come out from an outlet of the extraction vessel is collected.
EXAMPLE 8
Fresh oil palm leaves are grounded into powder form of 2 - 10mm length. 30g of the grounded oil palm leaves is loaded into an empty, 30mL extraction vessel. The extraction vessel is then heated to 40°C. Pressurized carbon dioxide (150 bars, - 4°C) and 0.2mL methanol are pumped into the vessel simultaneously for 3 hours. The extract containing the anti-oxidative compounds that come out from an outlet of the extraction vessel is collected.

Claims

1. A process for extracting water soluble compounds with anti-oxidative properties from oil palm biomass, the process includes the steps of:
i) reducing size of the oil palm biomass between 2- 10mm length;
ii) loading the oil palm biomass from step (i) into a vessel;
iii) subjecting the oil palm biomass with a stream of carbon dioxide having pressure range of 30-70MPa;
iv) collecting the water soluble compounds released from an outlet of the vessel, wherein the vessel is heated at a temperature range of 30-75°C upon loading of the oil palm biomass in the vessel; and
wherein the oil palm biomass is such as palm leaves, pressed fibre and empty fruit bunches.
2. A process for extracting water soluble compounds with anti-oxidative properties from oil palm biomass, the process includes the steps of:
i) reducing size of the oil palm biomass between 2- 10mm length;
ii) immersing the oil palm biomass in a solvent;
iii) loading the oil palm biomass from step (ii) into a vessel containing adsorbent; iv) subjecting the oil palm biomass with a stream of carbon dioxide having pressure range of 30-70MPa ;
v) collecting the water soluble compounds released from an outlet of the vessel, wherein the vessel is heated at a temperature range of 30-75°C upon loading of the oil palm biomass in the vessel; and
wherein the oil palm biomass is such as palm leaves, pressed fibre and empty fruit bunches.
3. The process for extracting water soluble compounds with anti-oxidative properties as claimed in claim 2 wherein the adsorbent is silica gel.
4. The process for extracting water soluble compounds with anti-oxidative properties as claimed in claim 2 wherein the solvent is alcohol.
5. A process for extracting water soluble compounds with anti-oxidative properties from oil palm biomass, the process includes the steps of:
i) reducing size of the oil palm biomass between 2- 10mm length;
ii) immersing the oil palm biomass in a solvent;
iii) loading the oil palm biomass from step (ii) into a vessel containing adsorbent; iv) subjecting the oil palm biomass with a stream of carbon dioxide having pressure range of 30-70MPa and a stream of solvent simultaneously; v) collecting the water soluble compounds released from an outlet of the vessel, wherein the vessel is heated at a temperature range of 30-75°C upon loading of the oil palm biomass in the vessel; and
wherein the oil palm biomass is such as palm leaves, pressed fibre and empty fruit bunches.
6. The process for extracting water soluble compounds with anti-oxidative properties as claimed in claim 5 wherein the adsorbent is silica gel.
7. The process for extracting water soluble compounds with anti-oxidative properties as claimed in claim 5 wherein the solvent is alcohol.
8. A process for extracting water soluble compounds with anti-oxidative properties from oil palm biomass, the process includes the steps of:
i) reducing size of the oil palm biomass between 2- 10mm length;
ii) immersing the oil palm biomass in a solvent;
iii) loading the oil palm biomass from step (ii) into a vessel containing adsorbent; iv) subjecting the oil palm biomass with a stream of carbon dioxide having pressure range of 30-70MPa and a stream of solvent simultaneously; v) collecting the water soluble compounds released from an outlet of the vessel, wherein the vessel is heated at a temperature range of 30-75°C upon loading of the oil palm biomass in the vessel; and
wherein the oil palm biomass is such as palm leaves, pressed fibre and empty fruit bunches.
9. The process for extracting water soluble compounds with anti-oxidative properties as claimed in claim 8 wherein the adsorbent is silica gel.
10. The process for extracting water soluble compounds with anti-oxidative properties as claimed in claim 8 wherein the solvent is alcohol.
11. A process for extracting water soluble compounds with anti-oxidative properties from oil palm biomass, the process includes the steps of:
i) reducing size of the oil palm biomass between 2- 10mm length;
ii) loading the oil palm biomass from step (i) into a vessel;
iii) subjecting the oil palm biomass with a stream of carbon dioxide having pressure range of 30-70MPa and a stream of solvent simultaneously; iv) collecting the water soluble compounds released from an outlet of the vessel, wherein the vessel is heated at a temperature range of 30-75°C upon loading of the oil palm biomass in the vessel; and
wherein the oil palm biomass is such as palm leaves, pressed fibre and empty fruit bunches.
12. The process for extracting water soluble compounds with anti-oxidative properties as claimed in claim 11 wherein the solvent is alcohol.
PCT/MY2011/000217 2010-10-12 2011-10-12 A process for extracting antioxidants from oil palm biomass WO2012050425A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SG2013027313A SG189364A1 (en) 2010-10-12 2011-10-12 A process for extracting antioxidants from oil palm biomass

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MYPI2010004801 2010-10-12
MYPI2010004801 2010-10-12

Publications (2)

Publication Number Publication Date
WO2012050425A2 true WO2012050425A2 (en) 2012-04-19
WO2012050425A3 WO2012050425A3 (en) 2012-06-07

Family

ID=45938834

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/MY2011/000217 WO2012050425A2 (en) 2010-10-12 2011-10-12 A process for extracting antioxidants from oil palm biomass

Country Status (3)

Country Link
MY (1) MY177259A (en)
SG (1) SG189364A1 (en)
WO (1) WO2012050425A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7161055B2 (en) * 2002-11-27 2007-01-09 Malaysian Palm Oil Board Method of extracting and isolating minor components from vegetable oil

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7161055B2 (en) * 2002-11-27 2007-01-09 Malaysian Palm Oil Board Method of extracting and isolating minor components from vegetable oil

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
LAU, H.L.N. ET AL. AMERICAN J. ENVIRONMENTAL SCIENCES. vol. 3, no. 2, 30 June 2007, pages 54 - 59 *
LAU, H.L.N. ET AL. J. AMERICAN OIL CHEMISTS' SOCIETY. vol. 83, no. 10, October 2006, pages 893 - 898 *
LAU, H.L.N. ET AL. J. FOOD ENGINEERING. vol. 84, 18 May 2007, pages 289 - 296 *

Also Published As

Publication number Publication date
WO2012050425A3 (en) 2012-06-07
MY177259A (en) 2020-09-10
SG189364A1 (en) 2013-05-31

Similar Documents

Publication Publication Date Title
CN105237430B (en) A kind of method that spicy components are extracted in the green pepper from rattan
WO2007112570A1 (en) Thermal extraction method and product
JP5902256B2 (en) Extraction method of ceramide and / or pectin from whole fruit apple and / or apple juice residue
CN102020869B (en) Method for extracting uranidin from marigold flower
CN111072618A (en) Method for conveniently and rapidly purifying dihydromyricetin from Ampelopsis grossedentata leaves
WO2017047191A1 (en) Polyisoprene production method
JP6154253B2 (en) Powdered or solid composition containing acylated sterol glycoside and production method thereof
WO2012050425A2 (en) A process for extracting antioxidants from oil palm biomass
JP6683450B2 (en) Extraction method of condensed tannin with high degree of polymerization
US8007838B2 (en) Process for producing a stable concentrated dietary supplement and supplement produced thereby
JP2015048329A5 (en) Powdered or solid composition containing acylated sterol glycoside and production method thereof
KR100814133B1 (en) Method for maca extracts with high content of macamide
JP2017137439A (en) Method of producing ceramide, and ceramide derivative
RU2011149200A (en) METHOD FOR PREPARING POLYPHENOL EXTRACTS FROM SPINAT LEAVES
KR20240009924A (en) Methods and systems for extraction of cannabinoids
EP4244201A1 (en) Production of cannabidiol from hemp using subcritical liquid carbon dioxide
WO2022018451A1 (en) Isolation of cannabinoids using mesoporous materials
CN112939744A (en) Efficient extraction method of cannabidiol
CN106629922A (en) Organics degrading agent and preparation method thereof
CN107669849B (en) An antiallergic agent prepared from coconut shell extract and rutin
KR100827072B1 (en) An efficent isolation process for betulin of high purity from birch bark
RU2279284C1 (en) Method for integrated utilization of larch wood
CN111253344A (en) Method for extracting paclitaxel from branches and leaves of taxus chinensis
US10982170B2 (en) Extraction methods of field muskmelon seed oil
CN111217865A (en) Method for preparing tea polyphenol by compound enzyme supercritical fluid extraction

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11832802

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 11832802

Country of ref document: EP

Kind code of ref document: A2